Restriction map for
pF1KA0739
(Circular) (Six-base) MAPSORT of: pF1KA0739.seq Check: 452 from: 1 to: 6326
pF1KA0739 6326 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2087 2087
Size: 6326
AccI GT'mk_AC
Cuts at: 1046 3293 3364 1046
Size: 2247 71 4008
Fragments arranged by size:
4008 2247 71
Acc65I G'GTAC_C
Cuts at: 3342 3342
Size: 6326
AclI AA'CG_TT
Cuts at: 6166 6166
Size: 6326
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 1507 1988 4151 4583 5330 5598 628
Size: 879 481 2163 432 747 268 1356
Fragments arranged by size:
2163 1356 879 747 481 432 268
AflIII A'CryG_T
Cuts at: 2745 3678 4782 5759 2745
Size: 933 1104 977 3312
Fragments arranged by size:
3312 1104 977 933
AgeI A'CCGG_T
Cuts at: 4714 4714
Size: 6326
AleI CACnn'nnGTG
Cuts at: 2410 2746 2410
Size: 336 5990
AlwNI CAG_nnn'CTG
Cuts at: 3631 5198 3631
Size: 1567 4759
ApaLI G'TGCA_C
Cuts at: 3043 5096 3043
Size: 2053 4273
ApoI r'AATT_y
Cuts at: 755 1067 1837 2951 3330 5679 5935 755
Size: 312 770 1114 379 2349 256 1146
Fragments arranged by size:
2349 1146 1114 770 379 312 256
AseI AT'TA_AT
Cuts at: 20 710 2504 20
Size: 690 1794 3842
Fragments arranged by size:
3842 1794 690
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
AvaI C'yCGr_G
Cuts at: 3346 3346
Size: 6326
BamHI G'GATC_C
Cuts at: 1392 3351 1392
Size: 1959 4367
BanI G'GyrC_C
Cuts at: 499 3342 3756 4004 4039 499
Size: 2843 414 248 35 2786
Fragments arranged by size:
2843 2786 414 248 35
BanII G_rGCy'C
Cuts at: 3340 4370 3340
Size: 1030 5296
BbeI G_GCGC'C
Cuts at: 3760 4008 3760
Size: 248 6078
BbsI GAAGACnn'nnnn_
Cuts at: 2859 2859
Size: 6326
BbvCI CC'TCA_GC
Cuts at: 905 905
Size: 6326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 352 386 352
Size: 34 6292
BciVI GTATCCnnnnn_n'
Cuts at: 166 4224 4985 6326 166
Size: 4058 761 1341 166
Fragments arranged by size:
4058 1341 761 166
BclI T'GATC_A
Cuts at: 3846 3846
Size: 6326
BfrBI ATG'CAT
Cuts at: 735 2214 735
Size: 1479 4847
BglII A'GATC_T
Cuts at: 554 3841 5878 554
Size: 3287 2037 1002
Fragments arranged by size:
3287 2037 1002
BlpI GC'TnA_GC
Cuts at: 3190 3438 3190
Size: 248 6078
Bme1580I G_kGCm'C
Cuts at: 3047 3951 4044 5100 3047
Size: 904 93 1056 4273
Fragments arranged by size:
4273 1056 904 93
BmrI ACTGGGnnnn_n'
Cuts at: 1085 1541 1757 2187 2456 3538 3619 3954 6098
Size: 456 216 430 269 1082 81 335 2144
Cuts at: 6098 1085
Size: 1313
Fragments arranged by size:
2144 1313 1082 456 430 335 269 216
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1169 1348 1624 4693 1169
Size: 179 276 3069 2802
Fragments arranged by size:
3069 2802 276 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 666 1139 1364 1475 1608 2119 2807 3089 666
Size: 473 225 111 133 511 688 282 3903
Fragments arranged by size:
3903 688 511 473 282 225 133 111
Bpu10I CC'TnA_GC
Cuts at: 694 905 2080 694
Size: 211 1175 4940
Fragments arranged by size:
4940 1175 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 2298 2617 2743 3502 4381 4873 5171 187
Size: 2111 319 126 759 879 492 298 1342
Fragments arranged by size:
2111 1342 879 759 492 319 298 126
BsaI GGTCTCn'nnnn_
Cuts at: 158 2035 6030 158
Size: 1877 3995 454
Fragments arranged by size:
3995 1877 454
BsaAI yAC'GTr
Cuts at: 3679 3679
Size: 6326
BsaBI GATnn'nnATC
Cuts at: 6 3840 5877 6
Size: 3834 2037 455
Fragments arranged by size:
3834 2037 455
BsaHI Gr'CG_yC
Cuts at: 3757 4005 4707 6203 3757
Size: 248 702 1496 3880
Fragments arranged by size:
3880 1496 702 248
BsaWI w'CCGG_w
Cuts at: 401 3515 4036 4714 4988 5135 5546 401
Size: 3114 521 678 274 147 411 1181
Fragments arranged by size:
3114 1181 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 948 978 948
Size: 30 6296
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 137 956 137
Size: 819 5507
BseYI C'CCAG_C
Cuts at: 2553 2682 3751 4509 5086 5582 2553
Size: 129 1069 758 577 496 3297
Fragments arranged by size:
3297 1069 758 577 496 129
BsiEI CG_ry'CG
Cuts at: 104 3868 3914 5122 104
Size: 3764 46 1208 1308
Fragments arranged by size:
3764 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1376 3047 3340 4118 4308 5100 1376
Size: 1671 293 778 190 792 2602
Fragments arranged by size:
2602 1671 792 778 293 190
BsmI GAATG_Cn'
Cuts at: 2206 2779 2206
Size: 573 5753
BsmBI CGTCTCn'nnnn_
Cuts at: 399 399
Size: 6326
Bsp1286I G_dGCh'C
Cuts at: 1376 3047 3340 3951 4044 4118 4308 4370 5100
Size: 1671 293 611 93 74 190 62 730
Cuts at: 5100 1376
Size: 2602
Fragments arranged by size:
2602 1671 730 611 293 190 93 74
62
BspEI T'CCGG_A
Cuts at: 5546 5546
Size: 6326
BspHI T'CATG_A
Cuts at: 528 1058 1277 1296 2870 5502 528
Size: 530 219 19 1574 2632 1352
Fragments arranged by size:
2632 1574 1352 530 219 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2087 3026 3376 3590 3892 4273 2087
Size: 939 350 214 302 381 4140
Fragments arranged by size:
4140 939 381 350 302 214
BsrBI CCG'CTC
Cuts at: 50 4618 4672 6156 6321 50
Size: 4568 54 1484 165 55
Fragments arranged by size:
4568 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 673 4238 673
Size: 3565 2761
BsrFI r'CCGG_y
Cuts at: 359 4324 4505 4714 359
Size: 3965 181 209 1971
Fragments arranged by size:
3965 1971 209 181
BssSI C'ACGA_G
Cuts at: 4597 4955 4597
Size: 358 5968
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 3703 1930
Size: 1773 4553
BstBI TT'CG_AA
Cuts at: 4687 4687
Size: 6326
BstXI CCAn_nnnn'nTGG
Cuts at: 1098 1255 1388 2682 2805 1098
Size: 157 133 1294 123 4619
Fragments arranged by size:
4619 1294 157 133 123
BstYI r'GATC_y
Cuts at: 1 554 1261 1392 2230 3165 3351 3818 3841
Size: 553 707 131 838 935 186 467 23
Cuts at: 3841 4176 4422 5434 5520 5532 5878 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 935 838 707 553 467 449 346
335 246 186 131 86 23 12
BstZ17I GTA'TAC
Cuts at: 3294 3294
Size: 6326
BtgI C'CryG_G
Cuts at: 1265 2369 1265
Size: 1104 5222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4276 4457 4620 4276
Size: 181 163 5982
Fragments arranged by size:
5982 181 163
BtsI GCAGTG_nn'
Cuts at: 3569 3569
Size: 6326
DraI TTT'AAA
Cuts at: 3325 3325
Size: 6326
DrdI GACnn_nn'nnGTC
Cuts at: 4032 4890 4032
Size: 858 5468
EaeI y'GGCC_r
Cuts at: 357 390 1268 2241 2672 3911 4085 4476 4503
Size: 33 878 973 431 1239 174 391 27
Cuts at: 4503 5848 357
Size: 1345 835
Fragments arranged by size:
1345 1239 973 878 835 431 391 174
33 27
EagI C'GGCC_G
Cuts at: 3911 3911
Size: 6326
EarI CTCTTCn'nnn_
Cuts at: 302 509 862 2564 3055 3260 4349 4559 302
Size: 207 353 1702 491 205 1089 210 2069
Fragments arranged by size:
2069 1702 1089 491 353 210 207 205
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4534 4844 4990 6136 4534
Size: 310 146 1146 4724
Fragments arranged by size:
4724 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3338 3338
Size: 6326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 666 1139 1364 1475 1507 1608 1988 2119
Size: 38 473 225 111 32 101 380 131
Cuts at: 2119 2807 3089 4151 4583 5330 5598 628
Size: 688 282 1062 432 747 268 1356
Fragments arranged by size:
1356 1062 747 688 473 432 380 282
268 225 131 111 101 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 1143 1360 1143
Size: 217 6109
EcoO109I rG'GnC_Cy
Cuts at: 1575 3302 3465 1575
Size: 1727 163 4436
Fragments arranged by size:
4436 1727 163
EcoRI G'AATT_C
Cuts at: 2951 3330 2951
Size: 379 5947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4345 4345
Size: 6326
FspI TGC'GCA
Cuts at: 3037 4107 3037
Size: 1070 5256
HaeII r_GCGC'y
Cuts at: 3760 4008 5030 6009 3760
Size: 248 1022 979 4077
Fragments arranged by size:
4077 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 457 489 759 791 1766 1798 2490 2522 4168
Size: 32 270 32 975 32 692 32 1646
Cuts at: 4168 4198 4200 4230 457
Size: 30 2 30 2553
Fragments arranged by size:
2553 1646 975 692 270 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 3068 3365 3068
Size: 297 6029
HindIII A'AGCT_T
Cuts at: 2449 2449
Size: 6326
KasI G'GCGC_C
Cuts at: 3756 4004 3756
Size: 248 6078
KpnI G_GTAC'C
Cuts at: 3346 3346
Size: 6326
MfeI C'AATT_G
Cuts at: 1718 1718
Size: 6326
MluI A'CGCG_T
Cuts at: 5759 5759
Size: 6326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1003 1083 3215 4997 5181 1003
Size: 80 2132 1782 184 2148
Fragments arranged by size:
2148 2132 1782 184 80
MscI TGG'CCA
Cuts at: 392 1270 2674 4087 392
Size: 878 1404 1413 2631
Fragments arranged by size:
2631 1413 1404 878
MslI CAynn'nnrTG
Cuts at: 857 1276 2056 2410 2746 4315 4442 857
Size: 419 780 354 336 1569 127 2741
Fragments arranged by size:
2741 1569 780 419 354 336 127
MspA1I CmG'CkG
Cuts at: 1159 2070 3033 3109 3437 3751 4111 5124 5369
Size: 911 963 76 328 314 360 1013 245
Cuts at: 5369 1159
Size: 2116
Fragments arranged by size:
2116 1013 963 911 360 328 314 245
76
NaeI GCC'GGC
Cuts at: 361 4507 361
Size: 4146 2180
NarI GG'CG_CC
Cuts at: 3757 4005 3757
Size: 248 6078
NcoI C'CATG_G
Cuts at: 1265 2369 1265
Size: 1104 5222
NgoMIV G'CCGG_C
Cuts at: 359 4505 359
Size: 4146 2180
NsiI A_TGCA'T
Cuts at: 737 2216 737
Size: 1479 4847
NspI r_CATG'y
Cuts at: 523 739 1659 2061 2749 3028 3379 3879 4786
Size: 216 920 402 688 279 351 500 907
Cuts at: 4786 523
Size: 2063
Fragments arranged by size:
2063 920 907 688 500 402 351 279
216
PciI A'CATG_T
Cuts at: 2745 4782 2745
Size: 2037 4289
PflMI CCAn_nnn'nTGG
Cuts at: 2525 2525
Size: 6326
PfoI T'CCnGG_A
Cuts at: 725 770 1380 2784 725
Size: 45 610 1404 4267
Fragments arranged by size:
4267 1404 610 45
PmeI GTTT'AAAC
Cuts at: 3325 3325
Size: 6326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2615 2647 5465 5497 2615
Size: 32 2818 32 3444
Fragments arranged by size:
3444 2818 32 32
PstI C_TGCA'G
Cuts at: 2098 3373 4058 2098
Size: 1275 685 4366
Fragments arranged by size:
4366 1275 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6326
PvuII CAG'CTG
Cuts at: 1159 2070 3109 3751 4111 1159
Size: 911 1039 642 360 3374
Fragments arranged by size:
3374 1039 911 642 360
RsrII CG'GwC_CG
Cuts at: 4521 4521
Size: 6326
SacI G_AGCT'C
Cuts at: 3340 3340
Size: 6326
SalI G'TCGA_C
Cuts at: 3363 3363
Size: 6326
SapI GCTCTTCn'nnn_
Cuts at: 302 509 2564 4349 4559 302
Size: 207 2055 1785 210 2069
Fragments arranged by size:
2069 2055 1785 210 207
SbfI CC_TGCA'GG
Cuts at: 3373 3373
Size: 6326
ScaI AGT'ACT
Cuts at: 1800 2140 2906 5843 1800
Size: 340 766 2937 2283
Fragments arranged by size:
2937 2283 766 340
SexAI A'CCwGG_T
Cuts at: 1361 1361
Size: 6326
SfcI C'TryA_G
Cuts at: 33 2094 2944 3369 4054 5047 5238 33
Size: 2061 850 425 685 993 191 1121
Fragments arranged by size:
2061 1121 993 850 685 425 191
SfoI GGC'GCC
Cuts at: 3758 4006 3758
Size: 248 6078
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6326
SmaI CCC'GGG
Cuts at: 3348 3348
Size: 6326
SmlI C'TyrA_G
Cuts at: 202 2313 2632 2758 3481 4396 4888 5150 202
Size: 2111 319 126 723 915 492 262 1378
Fragments arranged by size:
2111 1378 915 723 492 319 262 126
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6326
SphI G_CATG'C
Cuts at: 739 3379 3879 739
Size: 2640 500 3186
Fragments arranged by size:
3186 2640 500
StuI AGG'CCT
Cuts at: 446 1087 1175 2834 446
Size: 641 88 1659 3938
Fragments arranged by size:
3938 1659 641 88
StyI C'CwwG_G
Cuts at: 1265 2369 3140 3257 3460 3814 1265
Size: 1104 771 117 203 354 3777
Fragments arranged by size:
3777 1104 771 354 203 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 2674 3909 4042 4501 4710 1431
Size: 1243 1235 133 459 209 3047
Fragments arranged by size:
3047 1243 1235 459 209 133
TatI w'GTAC_w
Cuts at: 277 1643 1798 2101 2138 2566 2904 5841 277
Size: 1366 155 303 37 428 338 2937 762
Fragments arranged by size:
2937 1366 762 428 338 303 155 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5843 5875 5843
Size: 32 6294
Tth111I GACn'n_nGTC
Cuts at: 4123 4123
Size: 6326
XbaI T'CTAG_A
Cuts at: 3357 3357
Size: 6326
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 3821 2212
Size: 1609 4717
XmaI C'CCGG_G
Cuts at: 3346 3346
Size: 6326
XmnI GAAnn'nnTTC
Cuts at: 833 833
Size: 6326
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BglI BmgBI BmtI BsgI BsiWI BsrGI
BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI