Restriction map for
pF1KA0740
(Circular) (Six-base) MAPSORT of: pF1KA0740.seq Check: 4908 from: 1 to: 5201
pF1KA0740 5201 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 755 755
Size: 5201
AccI GT'mk_AC
Cuts at: 1088 2239 1088
Size: 1151 4050
Acc65I G'GTAC_C
Cuts at: 2072 2217 2072
Size: 145 5056
AclI AA'CG_TT
Cuts at: 140 1555 5041 140
Size: 1415 3486 300
Fragments arranged by size:
3486 1415 300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 907 1304 2098 3026 3458 3811 4473 778
Size: 129 397 794 928 432 353 662 1506
Fragments arranged by size:
1506 928 794 662 432 397 353 129
AfeI AGC'GCT
Cuts at: 728 728
Size: 5201
AflIII A'CryG_T
Cuts at: 2553 4353 4634 2553
Size: 1800 281 3120
Fragments arranged by size:
3120 1800 281
AgeI A'CCGG_T
Cuts at: 828 828
Size: 5201
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 5201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 763 763
Size: 5201
AlwNI CAG_nnn'CTG
Cuts at: 243 1204 2506 3944 243
Size: 961 1302 1438 1500
Fragments arranged by size:
1500 1438 1302 961
ApaI G_GGCC'C
Cuts at: 1117 1295 1117
Size: 178 5023
ApaLI G'TGCA_C
Cuts at: 4039 4039
Size: 5201
ApoI r'AATT_y
Cuts at: 839 953 979 2175 2205 4554 4810 839
Size: 114 26 1196 30 2349 256 1230
Fragments arranged by size:
2349 1230 1196 256 114 30 26
AseI AT'TA_AT
Cuts at: 20 1783 20
Size: 1763 3438
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5201
AvaI C'yCGr_G
Cuts at: 314 1426 2221 314
Size: 1112 795 3294
Fragments arranged by size:
3294 1112 795
AvrII C'CTAG_G
Cuts at: 1120 1120
Size: 5201
BamHI G'GATC_C
Cuts at: 2226 2226
Size: 5201
BanI G'GyrC_C
Cuts at: 524 575 743 798 2051 2072 2217 2631 2879
Size: 51 168 55 1253 21 145 414 248
Cuts at: 2879 2914 524
Size: 35 2811
Fragments arranged by size:
2811 1253 414 248 168 145 55 51
35 21
BanII G_rGCy'C
Cuts at: 1117 1295 2215 3245 1117
Size: 178 920 1030 3073
Fragments arranged by size:
3073 1030 920 178
BbeI G_GCGC'C
Cuts at: 579 2635 2883 579
Size: 2056 248 2897
Fragments arranged by size:
2897 2056 248
BbsI GAAGACnn'nnnn_
Cuts at: 193 1142 193
Size: 949 4252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2147 2181 2147
Size: 34 5167
BciVI GTATCCnnnnn_n'
Cuts at: 351 485 3099 4155 5201 351
Size: 134 2614 1056 1046 351
Fragments arranged by size:
2614 1056 1046 351 134
BclI T'GATC_A
Cuts at: 369 2721 369
Size: 2352 2849
BglII A'GATC_T
Cuts at: 2716 4753 2716
Size: 2037 3164
BlpI GC'TnA_GC
Cuts at: 2313 2313
Size: 5201
Bme1580I G_kGCm'C
Cuts at: 1117 1295 1602 2826 2919 4043 1117
Size: 178 307 1224 93 1124 2275
Fragments arranged by size:
2275 1224 1124 307 178 93
BmgBI CAC'GTC
Cuts at: 1566 1566
Size: 5201
BmrI ACTGGGnnnn_n'
Cuts at: 2413 2494 2829 4973 2413
Size: 81 335 2144 2641
Fragments arranged by size:
2641 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 359 1915 3568 359
Size: 1556 1653 1992
Fragments arranged by size:
1992 1653 1556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2168 1336
Size: 832 4369
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2136 2377 3256 3970 4268 2136
Size: 241 879 714 298 3069
Fragments arranged by size:
3069 879 714 298 241
BsaI GGTCTCn'nnnn_
Cuts at: 4905 4905
Size: 5201
BsaAI yAC'GTr
Cuts at: 255 1512 2554 255
Size: 1257 1042 2902
Fragments arranged by size:
2902 1257 1042
BsaBI GATnn'nnATC
Cuts at: 6 2715 4752 6
Size: 2709 2037 455
Fragments arranged by size:
2709 2037 455
BsaHI Gr'CG_yC
Cuts at: 576 2632 2880 3582 5078 576
Size: 2056 248 702 1496 699
Fragments arranged by size:
2056 1496 702 699 248
BsaWI w'CCGG_w
Cuts at: 828 1222 2390 2911 3589 4000 4147 4421 828
Size: 394 1168 521 678 411 147 274 1608
Fragments arranged by size:
1608 1168 678 521 411 394 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1123 1123
Size: 5201
BseYI C'CCAG_C
Cuts at: 2626 3384 4049 4457 2626
Size: 758 665 408 3370
Fragments arranged by size:
3370 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1217 1953 1217
Size: 736 4465
BsiEI CG_ry'CG
Cuts at: 104 2743 2789 4019 104
Size: 2639 46 1230 1286
Fragments arranged by size:
2639 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1901 2215 2993 3183 4043 1901
Size: 314 778 190 860 3059
Fragments arranged by size:
3059 860 778 314 190
Bsp1286I G_dGCh'C
Cuts at: 1117 1295 1602 1901 2215 2826 2919 2993 3183
Size: 178 307 299 314 611 93 74 190
Cuts at: 3183 3245 4043 1117
Size: 62 798 2275
Fragments arranged by size:
2275 798 611 314 307 299 190 178
93 74 62
BspHI T'CATG_A
Cuts at: 1463 1481 3633 1463
Size: 18 2152 3031
Fragments arranged by size:
3031 2152 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 755 2251 2465 2767 3148 392
Size: 363 1496 214 302 381 2445
Fragments arranged by size:
2445 1496 381 363 302 214
BsrBI CCG'CTC
Cuts at: 50 2049 3493 3547 5031 5196 50
Size: 1999 1444 54 1484 165 55
Fragments arranged by size:
1999 1484 1444 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3113 3113
Size: 5201
BsrFI r'CCGG_y
Cuts at: 828 3199 3380 828
Size: 2371 181 2649
Fragments arranged by size:
2649 2371 181
BssSI C'ACGA_G
Cuts at: 1869 3472 4180 1869
Size: 1603 708 2890
Fragments arranged by size:
2890 1603 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2578 2578
Size: 5201
BstBI TT'CG_AA
Cuts at: 3562 3562
Size: 5201
BstXI CCAn_nnnn'nTGG
Cuts at: 266 1302 266
Size: 1036 4165
BstYI r'GATC_y
Cuts at: 1 1764 2226 2693 2716 3051 3297 3603 3615
Size: 1763 462 467 23 335 246 306 12
Cuts at: 3615 3701 4753 1
Size: 86 1052 449
Fragments arranged by size:
1763 1052 467 462 449 335 306 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1130 1130
Size: 5201
BtgI C'CryG_G
Cuts at: 107 179 857 107
Size: 72 678 4451
Fragments arranged by size:
4451 678 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2131 3151 3332 3495 2131
Size: 1020 181 163 3837
Fragments arranged by size:
3837 1020 181 163
BtsI GCAGTG_nn'
Cuts at: 2195 2444 2195
Size: 249 4952
DraI TTT'AAA
Cuts at: 1575 2200 1575
Size: 625 4576
DrdI GACnn_nn'nnGTC
Cuts at: 1321 2907 4251 1321
Size: 1586 1344 2271
Fragments arranged by size:
2271 1586 1344
EaeI y'GGCC_r
Cuts at: 245 1943 2192 2786 2960 3351 3378 4723 245
Size: 1698 249 594 174 391 27 1345 723
Fragments arranged by size:
1698 1345 723 594 391 249 174 27
EagI C'GGCC_G
Cuts at: 2786 2786
Size: 5201
EarI CTCTTCn'nnn_
Cuts at: 975 3224 3434 975
Size: 2249 210 2742
Fragments arranged by size:
2742 2249 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1089 3409 4151 4297 5011 1089
Size: 2320 742 146 714 1279
Fragments arranged by size:
2320 1279 742 714 146
EcoICRI GAG'CTC
Cuts at: 2213 2213
Size: 5201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 778 907 1304 1336 2098 2168 3026 3458 3811
Size: 129 397 32 762 70 858 432 353
Cuts at: 3811 4473 778
Size: 662 1506
Fragments arranged by size:
1506 858 762 662 432 397 353 129
70 32
EcoNI CCTnn'n_nnAGG
Cuts at: 1168 1168
Size: 5201
EcoO109I rG'GnC_Cy
Cuts at: 1113 1291 1333 1422 2340 1113
Size: 178 42 89 918 3974
Fragments arranged by size:
3974 918 178 89 42
EcoRI G'AATT_C
Cuts at: 2175 2205 2175
Size: 30 5171
EcoRV GAT'ATC
Cuts at: 1709 1709
Size: 5201
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 210 1494 2184 3220 210
Size: 1284 690 1036 2191
Fragments arranged by size:
2191 1284 1036 690
FspI TGC'GCA
Cuts at: 2982 2982
Size: 5201
HaeII r_GCGC'y
Cuts at: 579 730 2635 2883 4113 4884 579
Size: 151 1905 248 1230 771 896
Fragments arranged by size:
1905 1230 896 771 248 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 423 455 3043 3073 3075 3105 423
Size: 32 2588 30 2 30 2519
Fragments arranged by size:
2588 2519 32 30 30 2
HincII GTy'rAC
Cuts at: 1089 1269 1856 2240 1089
Size: 180 587 384 4050
Fragments arranged by size:
4050 587 384 180
KasI G'GCGC_C
Cuts at: 575 2631 2879 575
Size: 2056 248 2897
Fragments arranged by size:
2897 2056 248
KpnI G_GTAC'C
Cuts at: 2076 2221 2076
Size: 145 5056
MluI A'CGCG_T
Cuts at: 4634 4634
Size: 5201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3960 4144 3960
Size: 184 5017
MscI TGG'CCA
Cuts at: 247 2962 247
Size: 2715 2486
MslI CAynn'nnrTG
Cuts at: 253 1246 1300 3190 3317 253
Size: 993 54 1890 127 2137
Fragments arranged by size:
2137 1890 993 127 54
MspA1I CmG'CkG
Cuts at: 240 1611 1776 2057 2312 2626 2986 3770 4015
Size: 1371 165 281 255 314 360 784 245
Cuts at: 4015 240
Size: 1426
Fragments arranged by size:
1426 1371 784 360 314 281 255 245
165
NaeI GCC'GGC
Cuts at: 3382 3382
Size: 5201
NarI GG'CG_CC
Cuts at: 576 2632 2880 576
Size: 2056 248 2897
Fragments arranged by size:
2897 2056 248
NcoI C'CATG_G
Cuts at: 107 857 107
Size: 750 4451
NdeI CA'TA_TG
Cuts at: 396 396
Size: 5201
NgoMIV G'CCGG_C
Cuts at: 3380 3380
Size: 5201
NspI r_CATG'y
Cuts at: 471 1251 1843 2254 2754 4357 471
Size: 780 592 411 500 1603 1315
Fragments arranged by size:
1603 1315 780 592 500 411
PciI A'CATG_T
Cuts at: 4353 4353
Size: 5201
PflMI CCAn_nnn'nTGG
Cuts at: 1941 1941
Size: 5201
PmeI GTTT'AAAC
Cuts at: 2200 2200
Size: 5201
PmlI CAC'GTG
Cuts at: 255 255
Size: 5201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 5169
PpuMI rG'GwC_Cy
Cuts at: 1422 1422
Size: 5201
PshAI GACnn'nnGTC
Cuts at: 1303 1303
Size: 5201
PspOMI G'GGCC_C
Cuts at: 1113 1291 1113
Size: 178 5023
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 638 670 638
Size: 32 5169
PstI C_TGCA'G
Cuts at: 2028 2248 2933 2028
Size: 220 685 4296
Fragments arranged by size:
4296 685 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5201
PvuII CAG'CTG
Cuts at: 240 2626 2986 240
Size: 2386 360 2455
Fragments arranged by size:
2455 2386 360
RsrII CG'GwC_CG
Cuts at: 3396 3396
Size: 5201
SacI G_AGCT'C
Cuts at: 2215 2215
Size: 5201
SalI G'TCGA_C
Cuts at: 1087 2238 1087
Size: 1151 4050
SapI GCTCTTCn'nnn_
Cuts at: 3224 3434 3224
Size: 210 4991
SbfI CC_TGCA'GG
Cuts at: 2248 2248
Size: 5201
ScaI AGT'ACT
Cuts at: 4718 4718
Size: 5201
SfcI C'TryA_G
Cuts at: 33 1620 2024 2244 2929 3897 4088 33
Size: 1587 404 220 685 968 191 1146
Fragments arranged by size:
1587 1146 968 685 404 220 191
SfoI GGC'GCC
Cuts at: 577 2633 2881 577
Size: 2056 248 2897
Fragments arranged by size:
2897 2056 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5201
SmaI CCC'GGG
Cuts at: 2223 2223
Size: 5201
SmlI C'TyrA_G
Cuts at: 1426 2151 2356 3271 3985 4247 1426
Size: 725 205 915 714 262 2380
Fragments arranged by size:
2380 915 725 714 262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5201
SphI G_CATG'C
Cuts at: 1251 1843 2254 2754 1251
Size: 592 411 500 3698
Fragments arranged by size:
3698 592 500 411
StyI C'CwwG_G
Cuts at: 107 857 1120 1790 2335 2689 107
Size: 750 263 670 545 354 2619
Fragments arranged by size:
2619 750 670 545 354 263
SwaI ATTT'AAAT
Cuts at: 1575 1575
Size: 5201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 157 265 512 2784 2917 3376 3585 157
Size: 108 247 2272 133 459 209 1773
Fragments arranged by size:
2272 1773 459 247 209 133 108
TatI w'GTAC_w
Cuts at: 4716 4716
Size: 5201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 1068 1100 4718 4750 497
Size: 32 539 32 3618 32 948
Fragments arranged by size:
3618 948 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2998 2998
Size: 5201
XbaI T'CTAG_A
Cuts at: 2232 2232
Size: 5201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1875 2696 1875
Size: 821 4380
XhoI C'TCGA_G
Cuts at: 1426 1426
Size: 5201
XmaI C'CCGG_G
Cuts at: 2221 2221
Size: 5201
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AflII AhdI AloI AscI BaeI BbvCI BfrBI
BglI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI XmnI ZraI