Restriction map for
pF1KA0743
(Circular) (Six-base) MAPSORT of: pF1KA0743.seq Check: 5411 from: 1 to: 6296
pF1KA0743 6296 bp
With 182 enzymes: SgfI *
September 18, 2009 16:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2556 2152
Size: 404 5892
AccI GT'mk_AC
Cuts at: 503 2836 3334 503
Size: 2333 498 3465
Fragments arranged by size:
3465 2333 498
Acc65I G'GTAC_C
Cuts at: 614 3312 614
Size: 2698 3598
AclI AA'CG_TT
Cuts at: 6136 6136
Size: 6296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1585 1633 2337 4121 4553 5300 5568 1585
Size: 48 704 1784 432 747 268 2313
Fragments arranged by size:
2313 1784 747 704 432 268 48
AflII C'TTAA_G
Cuts at: 1351 1351
Size: 6296
AflIII A'CryG_T
Cuts at: 725 3648 4752 5729 725
Size: 2923 1104 977 1292
Fragments arranged by size:
2923 1292 1104 977
AgeI A'CCGG_T
Cuts at: 4684 4684
Size: 6296
AhdI GACnn_n'nnGTC
Cuts at: 3153 3153
Size: 6296
AleI CACnn'nnGTG
Cuts at: 1909 2362 1909
Size: 453 5843
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 899
Size: 6296
AlwNI CAG_nnn'CTG
Cuts at: 3601 5168 3601
Size: 1567 4729
ApaLI G'TGCA_C
Cuts at: 5066 5066
Size: 6296
ApoI r'AATT_y
Cuts at: 3300 5649 5905 3300
Size: 2349 256 3691
Fragments arranged by size:
3691 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6296
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
AvaI C'yCGr_G
Cuts at: 1868 2021 3316 1868
Size: 153 1295 4848
Fragments arranged by size:
4848 1295 153
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1376 1409 1376
Size: 33 6263
BamHI G'GATC_C
Cuts at: 3321 3321
Size: 6296
BanI G'GyrC_C
Cuts at: 565 614 1000 1933 3312 3726 3974 4009 565
Size: 49 386 933 1379 414 248 35 2852
Fragments arranged by size:
2852 1379 933 414 386 248 49 35
BanII G_rGCy'C
Cuts at: 118 397 428 562 2696 3241 3310 4340 118
Size: 279 31 134 2134 545 69 1030 2074
Fragments arranged by size:
2134 2074 1030 545 279 134 69 31
BbeI G_GCGC'C
Cuts at: 3730 3978 3730
Size: 248 6048
BbsI GAAGACnn'nnnn_
Cuts at: 2448 2969 2448
Size: 521 5775
BbvCI CC'TCA_GC
Cuts at: 1379 1379
Size: 6296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1306 1340 2802 2836 90
Size: 34 1182 34 1462 34 3550
Fragments arranged by size:
3550 1462 1182 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1000 4194 4955 6296 1000
Size: 3194 761 1341 1000
Fragments arranged by size:
3194 1341 1000 761
BclI T'GATC_A
Cuts at: 1808 3816 1808
Size: 2008 4288
BfrBI ATG'CAT
Cuts at: 1095 1095
Size: 6296
BglII A'GATC_T
Cuts at: 3811 5848 3811
Size: 2037 4259
BlpI GC'TnA_GC
Cuts at: 3408 3408
Size: 6296
Bme1580I G_kGCm'C
Cuts at: 3921 4014 5070 3921
Size: 93 1056 5147
Fragments arranged by size:
5147 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1020 2129 3508 3589 3924 6068 1020
Size: 1109 1379 81 335 2144 1248
Fragments arranged by size:
2144 1379 1248 1109 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4663 4663
Size: 6296
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1152 1216 1245 1458 2384 2404 259
Size: 893 64 29 213 926 20 4151
Fragments arranged by size:
4151 926 893 213 64 29 20
Bpu10I CC'TnA_GC
Cuts at: 1045 1379 1633 1045
Size: 334 254 5708
Fragments arranged by size:
5708 334 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3472 4351 4843 5141 3472
Size: 879 492 298 4627
Fragments arranged by size:
4627 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 337 1270 1391 6000 337
Size: 933 121 4609 633
Fragments arranged by size:
4609 933 633 121
BsaAI yAC'GTr
Cuts at: 810 2261 3288 3649 810
Size: 1451 1027 361 3457
Fragments arranged by size:
3457 1451 1027 361
BsaBI GATnn'nnATC
Cuts at: 6 280 3810 5847 6
Size: 274 3530 2037 455
Fragments arranged by size:
3530 2037 455 274
BsaHI Gr'CG_yC
Cuts at: 2067 2667 3727 3975 4677 6173 2067
Size: 600 1060 248 702 1496 2190
Fragments arranged by size:
2190 1496 1060 702 600 248
BsaWI w'CCGG_w
Cuts at: 1003 3485 4006 4684 4958 5105 5516 1003
Size: 2482 521 678 274 147 411 1783
Fragments arranged by size:
2482 1783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1243 1273 1243
Size: 30 6266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 514 2506 514
Size: 1992 4304
BseYI C'CCAG_C
Cuts at: 111 1133 2320 3721 4479 5056 5552 111
Size: 1022 1187 1401 758 577 496 855
Fragments arranged by size:
1401 1187 1022 855 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 978
Size: 6296
BsiEI CG_ry'CG
Cuts at: 104 3838 3884 5092 104
Size: 3734 46 1208 1308
Fragments arranged by size:
3734 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2400 3241 3310 4088 4278 5070 2400
Size: 841 69 778 190 792 3626
Fragments arranged by size:
3626 841 792 778 190 69
BsmI GAATG_Cn'
Cuts at: 885 2906 885
Size: 2021 4275
BsmBI CGTCTCn'nnnn_
Cuts at: 3166 3166
Size: 6296
Bsp1286I G_dGCh'C
Cuts at: 118 397 428 562 2400 2696 3241 3310 3921
Size: 279 31 134 1838 296 545 69 611
Cuts at: 3921 4014 4088 4278 4340 5070 118
Size: 93 74 190 62 730 1344
Fragments arranged by size:
1838 1344 730 611 545 296 279 190
134 93 74 69 62 31
BspEI T'CCGG_A
Cuts at: 5516 5516
Size: 6296
BspHI T'CATG_A
Cuts at: 1439 3218 5472 1439
Size: 1779 2254 2263
Fragments arranged by size:
2263 2254 1779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1030 2152 2556 3346 3560 3862 4243 1030
Size: 1122 404 790 214 302 381 3083
Fragments arranged by size:
3083 1122 790 404 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2105 3248 4588 4642 6126 6291 50
Size: 2055 1143 1340 54 1484 165 55
Fragments arranged by size:
2055 1484 1340 1143 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1740 1742 2250 4208 1740
Size: 2 508 1958 3828
Fragments arranged by size:
3828 1958 508 2
BsrFI r'CCGG_y
Cuts at: 997 2606 4294 4475 4684 997
Size: 1609 1688 181 209 2609
Fragments arranged by size:
2609 1688 1609 209 181
BsrGI T'GTAC_A
Cuts at: 1064 1991 3128 1064
Size: 927 1137 4232
Fragments arranged by size:
4232 1137 927
BssSI C'ACGA_G
Cuts at: 2011 4567 4925 2011
Size: 2556 358 3382
Fragments arranged by size:
3382 2556 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3673 3673
Size: 6296
BstBI TT'CG_AA
Cuts at: 4657 4657
Size: 6296
BstXI CCAn_nnnn'nTGG
Cuts at: 1944 2689 1944
Size: 745 5551
BstYI r'GATC_y
Cuts at: 1 704 3321 3788 3811 4146 4392 5404 5490
Size: 703 2617 467 23 335 246 1012 86
Cuts at: 5490 5502 5848 1
Size: 12 346 449
Fragments arranged by size:
2617 1012 703 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 515 1085 1361 2345 2999 515
Size: 570 276 984 654 3812
Fragments arranged by size:
3812 984 654 570 276
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1578 4246 4427 4590 1578
Size: 2668 181 163 3284
Fragments arranged by size:
3284 2668 181 163
BtsI GCAGTG_nn'
Cuts at: 931 3539 931
Size: 2608 3688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1994 2029 1994
Size: 35 6261
DraI TTT'AAA
Cuts at: 3295 3295
Size: 6296
DrdI GACnn_nn'nnGTC
Cuts at: 4002 4860 4002
Size: 858 5438
EaeI y'GGCC_r
Cuts at: 317 1485 2302 2578 3249 3881 4055 4446 4473
Size: 1168 817 276 671 632 174 391 27
Cuts at: 4473 5818 317
Size: 1345 795
Fragments arranged by size:
1345 1168 817 795 671 632 391 276
174 27
EagI C'GGCC_G
Cuts at: 3881 3881
Size: 6296
EarI CTCTTCn'nnn_
Cuts at: 448 1652 1678 1720 2099 2677 3238 4319 4529
Size: 1204 26 42 379 578 561 1081 210
Cuts at: 4529 448
Size: 2215
Fragments arranged by size:
2215 1204 1081 578 561 379 210 42
26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3186 4504 4814 4960 6106 3186
Size: 1318 310 146 1146 3376
Fragments arranged by size:
3376 1318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3239 3308 3239
Size: 69 6227
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1152 1216 1245 1458 1585 1633 2337 2384
Size: 893 64 29 213 127 48 704 47
Cuts at: 2384 2404 4121 4553 5300 5568 259
Size: 20 1717 432 747 268 987
Fragments arranged by size:
1717 987 893 747 704 432 268 213
127 64 48 47 29 20
EcoNI CCTnn'n_nnAGG
Cuts at: 205 1026 205
Size: 821 5475
EcoO109I rG'GnC_Cy
Cuts at: 2131 3029 3155 3435 2131
Size: 898 126 280 4992
Fragments arranged by size:
4992 898 280 126
EcoRI G'AATT_C
Cuts at: 3300 3300
Size: 6296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4315 4315
Size: 6296
FspI TGC'GCA
Cuts at: 4077 4077
Size: 6296
HaeII r_GCGC'y
Cuts at: 2258 3730 3978 5000 5979 2258
Size: 1472 248 1022 979 2575
Fragments arranged by size:
2575 1472 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 544 576 628 660 1099 1131 3139 3171 4138
Size: 32 52 32 439 32 2008 32 967
Cuts at: 4138 4168 4170 4200 544
Size: 30 2 30 2640
Fragments arranged by size:
2640 2008 967 439 52 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1230 1358 2475 2615 3335 1230
Size: 128 1117 140 720 4191
Fragments arranged by size:
4191 1117 720 140 128
HpaI GTT'AAC
Cuts at: 1230 1358 2615 1230
Size: 128 1257 4911
Fragments arranged by size:
4911 1257 128
KasI G'GCGC_C
Cuts at: 3726 3974 3726
Size: 248 6048
KpnI G_GTAC'C
Cuts at: 618 3316 618
Size: 2698 3598
MluI A'CGCG_T
Cuts at: 5729 5729
Size: 6296
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2757 4967 5151 2757
Size: 2210 184 3902
Fragments arranged by size:
3902 2210 184
MscI TGG'CCA
Cuts at: 319 1487 2304 2580 4057 319
Size: 1168 817 276 1477 2558
Fragments arranged by size:
2558 1477 1168 817 276
MslI CAynn'nnrTG
Cuts at: 274 574 1066 1084 1090 1663 1909 2362 3217
Size: 300 492 18 6 573 246 453 855
Cuts at: 3217 4285 4412 274
Size: 1068 127 2158
Fragments arranged by size:
2158 1068 855 573 492 453 300 246
127 18 6
MspA1I CmG'CkG
Cuts at: 157 864 3407 3721 4081 5094 5339 157
Size: 707 2543 314 360 1013 245 1114
Fragments arranged by size:
2543 1114 1013 707 360 314 245
NaeI GCC'GGC
Cuts at: 2608 4477 2608
Size: 1869 4427
NarI GG'CG_CC
Cuts at: 3727 3975 3727
Size: 248 6048
NcoI C'CATG_G
Cuts at: 1085 1085
Size: 6296
NgoMIV G'CCGG_C
Cuts at: 2606 4475 2606
Size: 1869 4427
NsiI A_TGCA'T
Cuts at: 1097 1097
Size: 6296
NspI r_CATG'y
Cuts at: 729 1065 1992 2184 3349 3849 4756 729
Size: 336 927 192 1165 500 907 2269
Fragments arranged by size:
2269 1165 927 907 500 336 192
PciI A'CATG_T
Cuts at: 725 4752 725
Size: 4027 2269
PflMI CCAn_nnn'nTGG
Cuts at: 2407 2407
Size: 6296
PfoI T'CCnGG_A
Cuts at: 707 2400 3044 707
Size: 1693 644 3959
Fragments arranged by size:
3959 1693 644
PmeI GTTT'AAAC
Cuts at: 3295 3295
Size: 6296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5435 5467 5435
Size: 32 6264
PpuMI rG'GwC_Cy
Cuts at: 2131 3155 2131
Size: 1024 5272
PshAI GACnn'nnGTC
Cuts at: 233 233
Size: 6296
PsiI TTA'TAA
Cuts at: 218 218
Size: 6296
PstI C_TGCA'G
Cuts at: 1496 2567 3343 4028 1496
Size: 1071 776 685 3764
Fragments arranged by size:
3764 1071 776 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6296
PvuII CAG'CTG
Cuts at: 864 3721 4081 864
Size: 2857 360 3079
Fragments arranged by size:
3079 2857 360
RsrII CG'GwC_CG
Cuts at: 4491 4491
Size: 6296
SacI G_AGCT'C
Cuts at: 3241 3310 3241
Size: 69 6227
SalI G'TCGA_C
Cuts at: 3333 3333
Size: 6296
SapI GCTCTTCn'nnn_
Cuts at: 2099 3238 4319 4529 2099
Size: 1139 1081 210 3866
Fragments arranged by size:
3866 1139 1081 210
SbfI CC_TGCA'GG
Cuts at: 2567 3343 2567
Size: 776 5520
ScaI AGT'ACT
Cuts at: 2717 5813 2717
Size: 3096 3200
SfcI C'TryA_G
Cuts at: 33 1492 2563 2600 2897 3339 4024 5017 5208
Size: 1459 1071 37 297 442 685 993 191
Cuts at: 5208 33
Size: 1121
Fragments arranged by size:
1459 1121 1071 993 685 442 297 191
37
SfoI GGC'GCC
Cuts at: 3728 3976 3728
Size: 248 6048
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6296
SmaI CCC'GGG
Cuts at: 3318 3318
Size: 6296
SmlI C'TyrA_G
Cuts at: 1351 2021 3451 4366 4858 5120 1351
Size: 670 1430 915 492 262 2527
Fragments arranged by size:
2527 1430 915 670 492 262
SnaBI TAC'GTA
Cuts at: 2261 2261
Size: 6296
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6296
SphI G_CATG'C
Cuts at: 2184 3349 3849 2184
Size: 1165 500 4631
Fragments arranged by size:
4631 1165 500
SspI AAT'ATT
Cuts at: 2748 2748
Size: 6296
StyI C'CwwG_G
Cuts at: 1085 1983 2676 3430 3784 1085
Size: 898 693 754 354 3597
Fragments arranged by size:
3597 898 754 693 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3879 4012 4471 4680 3879
Size: 133 459 209 5495
Fragments arranged by size:
5495 459 209 133
TatI w'GTAC_w
Cuts at: 539 1064 1991 2715 2986 3128 3134 5811 539
Size: 525 927 724 271 142 6 2677 1024
Fragments arranged by size:
2677 1024 927 724 525 271 142 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2265 2297 5813 5845 2265
Size: 32 3516 32 2716
Fragments arranged by size:
3516 2716 32 32
Tth111I GACn'n_nGTC
Cuts at: 1603 4093 1603
Size: 2490 3806
XbaI T'CTAG_A
Cuts at: 3327 3327
Size: 6296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1141 2565 2862 3791 1141
Size: 1424 297 929 3646
Fragments arranged by size:
3646 1424 929 297
XhoI C'TCGA_G
Cuts at: 2021 2021
Size: 6296
XmaI C'CCGG_G
Cuts at: 3316 3316
Size: 6296
XmnI GAAnn'nnTTC
Cuts at: 979 979
Size: 6296
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AloI ApaI AscI AvrII BglI BmgBI
BmtI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoRV FseI FspAI HindIII MfeI NdeI NheI NotI
NruI PacI PasI PmlI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI ZraI