Restriction map for
pF1KA0744
(Circular) (Six-base) MAPSORT of: pF1KA0744.seq Check: 8404 from: 1 to: 4883
pF1KA0744 4883 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1921 1921
Size: 4883
Acc65I G'GTAC_C
Cuts at: 1414 1899 1414
Size: 485 4398
AclI AA'CG_TT
Cuts at: 4723 4723
Size: 4883
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1612 1618 2708 3140 3887 4155 340
Size: 1272 6 1090 432 747 268 1068
Fragments arranged by size:
1272 1090 1068 747 432 268 6
AflIII A'CryG_T
Cuts at: 2235 3339 4316 2235
Size: 1104 977 2802
Fragments arranged by size:
2802 1104 977
AgeI A'CCGG_T
Cuts at: 3271 3271
Size: 4883
AlwNI CAG_nnn'CTG
Cuts at: 297 423 925 2188 3755 297
Size: 126 502 1263 1567 1425
Fragments arranged by size:
1567 1425 1263 502 126
ApaLI G'TGCA_C
Cuts at: 3653 3653
Size: 4883
ApoI r'AATT_y
Cuts at: 1140 1394 1887 4236 4492 1140
Size: 254 493 2349 256 1531
Fragments arranged by size:
2349 1531 493 256 254
AseI AT'TA_AT
Cuts at: 20 20
Size: 4883
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
AvaI C'yCGr_G
Cuts at: 1903 1903
Size: 4883
BamHI G'GATC_C
Cuts at: 1908 1908
Size: 4883
BanI G'GyrC_C
Cuts at: 1414 1899 2313 2561 2596 1414
Size: 485 414 248 35 3701
Fragments arranged by size:
3701 485 414 248 35
BanII G_rGCy'C
Cuts at: 167 773 1897 2927 167
Size: 606 1124 1030 2123
Fragments arranged by size:
2123 1124 1030 606
BbeI G_GCGC'C
Cuts at: 2317 2565 2317
Size: 248 4635
BbsI GAAGACnn'nnnn_
Cuts at: 1664 1664
Size: 4883
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1684 1718 90
Size: 34 1560 34 3255
Fragments arranged by size:
3255 1560 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2781 3542 4883 2781
Size: 761 1341 2781
Fragments arranged by size:
2781 1341 761
BclI T'GATC_A
Cuts at: 119 2403 119
Size: 2284 2599
BglII A'GATC_T
Cuts at: 2398 4435 2398
Size: 2037 2846
BlpI GC'TnA_GC
Cuts at: 350 1487 1995 350
Size: 1137 508 3238
Fragments arranged by size:
3238 1137 508
Bme1580I G_kGCm'C
Cuts at: 2508 2601 3657 2508
Size: 93 1056 3734
Fragments arranged by size:
3734 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 926 1449 1732 2095 2176 2511 4655 926
Size: 523 283 363 81 335 2144 1154
Fragments arranged by size:
2144 1154 523 363 335 283 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 186 3250 186
Size: 3064 1819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 445 902 1835 181
Size: 264 457 933 3229
Fragments arranged by size:
3229 933 457 264
Bpu10I CC'TnA_GC
Cuts at: 1180 1180
Size: 4883
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1633 2059 2938 3430 3728 1633
Size: 426 879 492 298 2788
Fragments arranged by size:
2788 879 492 426 298
BsaI GGTCTCn'nnnn_
Cuts at: 4587 4587
Size: 4883
BsaAI yAC'GTr
Cuts at: 2236 2236
Size: 4883
BsaBI GATnn'nnATC
Cuts at: 6 2397 4434 6
Size: 2391 2037 455
Fragments arranged by size:
2391 2037 455
BsaHI Gr'CG_yC
Cuts at: 2314 2562 3264 4760 2314
Size: 248 702 1496 2437
Fragments arranged by size:
2437 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2072 2593 3271 3545 3692 4103 2072
Size: 521 678 274 147 411 2852
Fragments arranged by size:
2852 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 386 416 386
Size: 30 4853
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 473 831 473
Size: 358 4525
BseYI C'CCAG_C
Cuts at: 1124 2308 3066 3643 4139 1124
Size: 1184 758 577 496 1868
Fragments arranged by size:
1868 1184 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1445
Size: 4883
BsiEI CG_ry'CG
Cuts at: 104 2425 2471 3679 104
Size: 2321 46 1208 1308
Fragments arranged by size:
2321 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 726 1480 1897 2675 2865 3657 726
Size: 754 417 778 190 792 1952
Fragments arranged by size:
1952 792 778 754 417 190
BsmI GAATG_Cn'
Cuts at: 1031 1031
Size: 4883
BsmBI CGTCTCn'nnnn_
Cuts at: 1162 1162
Size: 4883
Bsp1286I G_dGCh'C
Cuts at: 167 726 773 1480 1897 2508 2601 2675 2865
Size: 559 47 707 417 611 93 74 190
Cuts at: 2865 2927 3657 167
Size: 62 730 1393
Fragments arranged by size:
1393 730 707 611 559 417 190 93
74 62 47
BspEI T'CCGG_A
Cuts at: 4103 4103
Size: 4883
BspHI T'CATG_A
Cuts at: 1041 4059 1041
Size: 3018 1865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1067 1933 2147 2449 2830 1067
Size: 866 214 302 381 3120
Fragments arranged by size:
3120 866 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3175 3229 4713 4878 50
Size: 3125 54 1484 165 55
Fragments arranged by size:
3125 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2795 2795
Size: 4883
BsrFI r'CCGG_y
Cuts at: 2881 3062 3271 2881
Size: 181 209 4493
Fragments arranged by size:
4493 209 181
BssSI C'ACGA_G
Cuts at: 3154 3512 3154
Size: 358 4525
BstAPI GCAn_nnn'nTGC
Cuts at: 730 2260 730
Size: 1530 3353
BstBI TT'CG_AA
Cuts at: 753 1138 1573 3244 753
Size: 385 435 1671 2392
Fragments arranged by size:
2392 1671 435 385
BstYI r'GATC_y
Cuts at: 1 1550 1784 1908 2375 2398 2733 2979 3991
Size: 1549 234 124 467 23 335 246 1012
Cuts at: 3991 4077 4089 4435 1
Size: 86 12 346 449
Fragments arranged by size:
1549 1012 467 449 346 335 246 234
124 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 184 196 184
Size: 12 4871
BtgI C'CryG_G
Cuts at: 212 212
Size: 4883
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 445 1663 2833 3014 3177 445
Size: 1218 1170 181 163 2151
Fragments arranged by size:
2151 1218 1170 181 163
BtsI GCAGTG_nn'
Cuts at: 524 1121 1707 2126 524
Size: 597 586 419 3281
Fragments arranged by size:
3281 597 586 419
DraI TTT'AAA
Cuts at: 1882 1882
Size: 4883
DrdI GACnn_nn'nnGTC
Cuts at: 226 2589 3447 226
Size: 2363 858 1662
Fragments arranged by size:
2363 1662 858
EaeI y'GGCC_r
Cuts at: 2468 2642 3033 3060 4405 2468
Size: 174 391 27 1345 2946
Fragments arranged by size:
2946 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2468 2468
Size: 4883
EarI CTCTTCn'nnn_
Cuts at: 1622 2906 3116 1622
Size: 1284 210 3389
Fragments arranged by size:
3389 1284 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 855 3091 3401 3547 4693 855
Size: 2236 310 146 1146 1045
Fragments arranged by size:
2236 1146 1045 310 146
EcoICRI GAG'CTC
Cuts at: 1895 1895
Size: 4883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 340 445 902 1612 1618 1835 2708 3140
Size: 159 105 457 710 6 217 873 432
Cuts at: 3140 3887 4155 181
Size: 747 268 909
Fragments arranged by size:
909 873 747 710 457 432 268 217
159 105 6
EcoNI CCTnn'n_nnAGG
Cuts at: 409 488 834 409
Size: 79 346 4458
Fragments arranged by size:
4458 346 79
EcoO109I rG'GnC_Cy
Cuts at: 2022 2022
Size: 4883
EcoRI G'AATT_C
Cuts at: 1140 1887 1140
Size: 747 4136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2902 2902
Size: 4883
FspI TGC'GCA
Cuts at: 2664 2664
Size: 4883
HaeII r_GCGC'y
Cuts at: 1669 2317 2565 3587 4566 1669
Size: 648 248 1022 979 1986
Fragments arranged by size:
1986 1022 979 648 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 606 638 655 687 1205 1237 2725
Size: 32 450 32 17 32 518 32 1488
Cuts at: 2725 2755 2757 2787 124
Size: 30 2 30 2220
Fragments arranged by size:
2220 1488 518 450 32 32 32 32
30 30 17 2
HincII GTy'rAC
Cuts at: 1922 1922
Size: 4883
HindIII A'AGCT_T
Cuts at: 544 1333 544
Size: 789 4094
KasI G'GCGC_C
Cuts at: 2313 2561 2313
Size: 248 4635
KpnI G_GTAC'C
Cuts at: 1418 1903 1418
Size: 485 4398
MfeI C'AATT_G
Cuts at: 241 241
Size: 4883
MluI A'CGCG_T
Cuts at: 4316 4316
Size: 4883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 613 3554 3738 613
Size: 2941 184 1758
Fragments arranged by size:
2941 1758 184
MscI TGG'CCA
Cuts at: 2644 2644
Size: 4883
MslI CAynn'nnrTG
Cuts at: 1244 2872 2999 1244
Size: 1628 127 3128
Fragments arranged by size:
3128 1628 127
MspA1I CmG'CkG
Cuts at: 1491 1994 2308 2668 3681 3926 1491
Size: 503 314 360 1013 245 2448
Fragments arranged by size:
2448 1013 503 360 314 245
NaeI GCC'GGC
Cuts at: 3064 3064
Size: 4883
NarI GG'CG_CC
Cuts at: 2314 2562 2314
Size: 248 4635
NgoMIV G'CCGG_C
Cuts at: 3062 3062
Size: 4883
NspI r_CATG'y
Cuts at: 1936 2436 3343 1936
Size: 500 907 3476
Fragments arranged by size:
3476 907 500
PciI A'CATG_T
Cuts at: 3339 3339
Size: 4883
PflMI CCAn_nnn'nTGG
Cuts at: 595 632 925 946 1794 595
Size: 37 293 21 848 3684
Fragments arranged by size:
3684 848 293 37 21
PfoI T'CCnGG_A
Cuts at: 1787 1851 1787
Size: 64 4819
PmeI GTTT'AAAC
Cuts at: 1882 1882
Size: 4883
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 4022
Size: 32 4851
PstI C_TGCA'G
Cuts at: 1298 1930 2615 1298
Size: 632 685 3566
Fragments arranged by size:
3566 685 632
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4883
PvuII CAG'CTG
Cuts at: 1491 2308 2668 1491
Size: 817 360 3706
Fragments arranged by size:
3706 817 360
RsrII CG'GwC_CG
Cuts at: 3078 3078
Size: 4883
SacI G_AGCT'C
Cuts at: 1897 1897
Size: 4883
SalI G'TCGA_C
Cuts at: 1920 1920
Size: 4883
SapI GCTCTTCn'nnn_
Cuts at: 1622 2906 3116 1622
Size: 1284 210 3389
Fragments arranged by size:
3389 1284 210
SbfI CC_TGCA'GG
Cuts at: 1930 1930
Size: 4883
ScaI AGT'ACT
Cuts at: 4400 4400
Size: 4883
SfcI C'TryA_G
Cuts at: 33 1294 1926 2611 3604 3795 33
Size: 1261 632 685 993 191 1121
Fragments arranged by size:
1261 1121 993 685 632 191
SfoI GGC'GCC
Cuts at: 2315 2563 2315
Size: 248 4635
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4883
SmaI CCC'GGG
Cuts at: 1905 1905
Size: 4883
SmlI C'TyrA_G
Cuts at: 1612 2038 2953 3445 3707 1612
Size: 426 915 492 262 2788
Fragments arranged by size:
2788 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4883
SphI G_CATG'C
Cuts at: 1936 2436 1936
Size: 500 4383
StyI C'CwwG_G
Cuts at: 1100 1376 2017 2371 1100
Size: 276 641 354 3612
Fragments arranged by size:
3612 641 354 276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1284 2466 2599 3058 3267 1284
Size: 1182 133 459 209 2900
Fragments arranged by size:
2900 1182 459 209 133
TatI w'GTAC_w
Cuts at: 526 707 4398 526
Size: 181 3691 1011
Fragments arranged by size:
3691 1011 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4400 4432 4400
Size: 32 4851
Tth111I GACn'n_nGTC
Cuts at: 2680 2680
Size: 4883
XbaI T'CTAG_A
Cuts at: 1914 1914
Size: 4883
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1098 2378 1098
Size: 1280 3603
XmaI C'CCGG_G
Cuts at: 1903 1903
Size: 4883
XmnI GAAnn'nnTTC
Cuts at: 257 1631 257
Size: 1374 3509
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI BsiWI BsrGI BssHII BstEII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI