Restriction map for
pF1KA0756
(Circular) (Six-base) MAPSORT of: pF1KA0756.seq Check: 1425 from: 1 to: 6620
pF1KA0756 6620 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 483 3120 483
Size: 2637 3983
AccI GT'mk_AC
Cuts at: 2474 2642 3658 2474
Size: 168 1016 5436
Fragments arranged by size:
5436 1016 168
Acc65I G'GTAC_C
Cuts at: 3636 3636
Size: 6620
AclI AA'CG_TT
Cuts at: 6460 6460
Size: 6620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1834 2606 2611 2731 3368 4445 4877 5624 5892
Size: 772 5 120 637 1077 432 747 268
Cuts at: 5892 1834
Size: 2562
Fragments arranged by size:
2562 1077 772 747 637 432 268 120
5
AfeI AGC'GCT
Cuts at: 2251 2251
Size: 6620
AflII C'TTAA_G
Cuts at: 819 819
Size: 6620
AflIII A'CryG_T
Cuts at: 651 836 1910 1912 3972 5076 6053 651
Size: 185 1074 2 2060 1104 977 1218
Fragments arranged by size:
2060 1218 1104 1074 977 185 2
AgeI A'CCGG_T
Cuts at: 5008 5008
Size: 6620
AhdI GACnn_n'nnGTC
Cuts at: 3093 3093
Size: 6620
AleI CACnn'nnGTG
Cuts at: 1753 1753
Size: 6620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1771 1771
Size: 6620
AlwNI CAG_nnn'CTG
Cuts at: 3925 5492 3925
Size: 1567 5053
ApaI G_GGCC'C
Cuts at: 2527 2527
Size: 6620
ApaLI G'TGCA_C
Cuts at: 5390 5390
Size: 6620
ApoI r'AATT_y
Cuts at: 185 457 3624 5973 6229 185
Size: 272 3167 2349 256 576
Fragments arranged by size:
3167 2349 576 272 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6620
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6620
AvaI C'yCGr_G
Cuts at: 353 848 1723 1988 2044 2275 2411 3640 353
Size: 495 875 265 56 231 136 1229 3333
Fragments arranged by size:
3333 1229 875 495 265 231 136 56
AvrII C'CTAG_G
Cuts at: 2899 2899
Size: 6620
BamHI G'GATC_C
Cuts at: 482 2039 3645 482
Size: 1557 1606 3457
Fragments arranged by size:
3457 1606 1557
BanI G'GyrC_C
Cuts at: 892 1278 1747 3433 3636 4050 4298 4333 892
Size: 386 469 1686 203 414 248 35 3179
Fragments arranged by size:
3179 1686 469 414 386 248 203 35
BanII G_rGCy'C
Cuts at: 635 2446 2527 3139 3634 4664 635
Size: 1811 81 612 495 1030 2591
Fragments arranged by size:
2591 1811 1030 612 495 81
BbeI G_GCGC'C
Cuts at: 4054 4302 4054
Size: 248 6372
BbsI GAAGACnn'nnnn_
Cuts at: 1073 1865 2102 3356 3512 1073
Size: 792 237 1254 156 4181
Fragments arranged by size:
4181 1254 792 237 156
BbvCI CC'TCA_GC
Cuts at: 3115 3115
Size: 6620
BciVI GTATCCnnnnn_n'
Cuts at: 893 2980 4518 5279 6620 893
Size: 2087 1538 761 1341 893
Fragments arranged by size:
2087 1538 1341 893 761
BclI T'GATC_A
Cuts at: 4140 4140
Size: 6620
BfrBI ATG'CAT
Cuts at: 944 944
Size: 6620
BglI GCCn_nnn'nGGC
Cuts at: 1983 2359 2771 1983
Size: 376 412 5832
Fragments arranged by size:
5832 412 376
BglII A'GATC_T
Cuts at: 4135 6172 4135
Size: 2037 4583
BlpI GC'TnA_GC
Cuts at: 3732 3732
Size: 6620
Bme1580I G_kGCm'C
Cuts at: 849 895 1750 2483 2527 2898 4245 4338 5394
Size: 46 855 733 44 371 1347 93 1056
Cuts at: 5394 849
Size: 2075
Fragments arranged by size:
2075 1347 1056 855 733 371 93 46
44
BmgBI CAC'GTC
Cuts at: 2295 3584 2295
Size: 1289 5331
BmrI ACTGGGnnnn_n'
Cuts at: 2464 2885 3307 3832 3913 4248 6392 2464
Size: 421 422 525 81 335 2144 2692
Fragments arranged by size:
2692 2144 525 422 421 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1086 2426 3387 4987 1086
Size: 1340 961 1600 2719
Fragments arranged by size:
2719 1600 1340 961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1708 1783 2017 2712 2833 2950 553
Size: 1155 75 234 695 121 117 4223
Fragments arranged by size:
4223 1155 695 234 121 117 75
Bpu10I CC'TnA_GC
Cuts at: 2528 2960 3115 2528
Size: 432 155 6033
Fragments arranged by size:
6033 432 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 3796 4675 5167 5465 766
Size: 3030 879 492 298 1921
Fragments arranged by size:
3030 1921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 668 1514 2851 6324 668
Size: 846 1337 3473 964
Fragments arranged by size:
3473 1337 964 846
BsaAI yAC'GTr
Cuts at: 1913 2336 2454 2478 3973 1913
Size: 423 118 24 1495 4560
Fragments arranged by size:
4560 1495 423 118 24
BsaBI GATnn'nnATC
Cuts at: 6 4134 6171 6
Size: 4128 2037 455
Fragments arranged by size:
4128 2037 455
BsaHI Gr'CG_yC
Cuts at: 2341 4051 4299 5001 6497 2341
Size: 1710 248 702 1496 2464
Fragments arranged by size:
2464 1710 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1715 2554 3809 4330 5008 5282 5429 5840
Size: 237 839 1255 521 678 274 147 411
Cuts at: 5840 1478
Size: 2258
Fragments arranged by size:
2258 1255 839 678 521 411 274 237
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1771 1801 2320 2350 1771
Size: 30 519 30 6041
Fragments arranged by size:
6041 519 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 151 381 384 497 866 2034 3172 3306
Size: 9 230 3 113 369 1168 1138 134
Cuts at: 3306 142
Size: 3456
Fragments arranged by size:
3456 1168 1138 369 230 134 113 9
3
BseYI C'CCAG_C
Cuts at: 301 2233 4045 4803 5380 5876 301
Size: 1932 1812 758 577 496 1045
Fragments arranged by size:
1932 1812 1045 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1777 1777
Size: 6620
BsiEI CG_ry'CG
Cuts at: 104 415 4162 4208 5416 104
Size: 311 3747 46 1208 1308
Fragments arranged by size:
3747 1308 1208 311 46
BsiHKAI G_wGCw'C
Cuts at: 2271 3634 4412 4602 5394 2271
Size: 1363 778 190 792 3497
Fragments arranged by size:
3497 1363 792 778 190
BsmI GAATG_Cn'
Cuts at: 946 2355 946
Size: 1409 5211
BsmBI CGTCTCn'nnnn_
Cuts at: 1809 1809
Size: 6620
Bsp1286I G_dGCh'C
Cuts at: 635 849 895 1750 2271 2446 2483 2527 2898
Size: 214 46 855 521 175 37 44 371
Cuts at: 2898 3139 3634 4245 4338 4412 4602 4664 5394
Size: 241 495 611 93 74 190 62 730
Cuts at: 5394 635
Size: 1861
Fragments arranged by size:
1861 855 730 611 521 495 371 241
214 190 175 93 74 62 46 44
37
BspEI T'CCGG_A
Cuts at: 2554 5840 2554
Size: 3286 3334
BspHI T'CATG_A
Cuts at: 2603 2978 5796 2603
Size: 375 2818 3427
Fragments arranged by size:
3427 2818 375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 483 1397 2384 3120 3670 3884 4186 4567 483
Size: 914 987 736 550 214 302 381 2536
Fragments arranged by size:
2536 987 914 736 550 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1830 1893 4912 4966 6450 6615 50
Size: 1780 63 3019 54 1484 165 55
Fragments arranged by size:
3019 1780 1484 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2201 4532 2201
Size: 2331 4289
BsrFI r'CCGG_y
Cuts at: 1982 4618 4799 5008 1982
Size: 2636 181 209 3594
Fragments arranged by size:
3594 2636 209 181
BsrGI T'GTAC_A
Cuts at: 839 839
Size: 6620
BssSI C'ACGA_G
Cuts at: 4891 5249 4891
Size: 358 6262
BstAPI GCAn_nnn'nTGC
Cuts at: 708 3997 708
Size: 3289 3331
BstBI TT'CG_AA
Cuts at: 4981 4981
Size: 6620
BstEII G'GTnAC_C
Cuts at: 2758 2758
Size: 6620
BstXI CCAn_nnnn'nTGG
Cuts at: 1104 1659 1104
Size: 555 6065
BstYI r'GATC_y
Cuts at: 1 195 482 1709 2039 2489 3645 4112 4135
Size: 194 287 1227 330 450 1156 467 23
Cuts at: 4135 4470 4716 5728 5814 5826 6172 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1227 1156 1012 467 450 449 346 335
330 287 246 194 86 23 12
BtgI C'CryG_G
Cuts at: 107 626 1751 2769 107
Size: 519 1125 1018 3958
Fragments arranged by size:
3958 1125 1018 519
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 324 930 957 1542 3192 3231 4570 4751 4914
Size: 606 27 585 1650 39 1339 181 163
Cuts at: 4914 324
Size: 2030
Fragments arranged by size:
2030 1650 1339 606 585 181 163 39
27
BtsI GCAGTG_nn'
Cuts at: 408 1522 2859 3863 408
Size: 1114 1337 1004 3165
Fragments arranged by size:
3165 1337 1114 1004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 972 1007 972
Size: 35 6585
DraI TTT'AAA
Cuts at: 3619 3619
Size: 6620
DraIII CAC_nnn'GTG
Cuts at: 246 2438 246
Size: 2192 4428
DrdI GACnn_nn'nnGTC
Cuts at: 652 1211 2080 4326 5184 652
Size: 559 869 2246 858 2088
Fragments arranged by size:
2246 2088 869 858 559
EaeI y'GGCC_r
Cuts at: 110 412 1304 1394 1736 1946 1975 2012 3522
Size: 302 892 90 342 210 29 37 1510
Cuts at: 3522 3611 4205 4379 4770 4797 6142 110
Size: 89 594 174 391 27 1345 588
Fragments arranged by size:
1510 1345 892 594 588 391 342 302
210 174 90 89 37 29 27
EagI C'GGCC_G
Cuts at: 412 4205 412
Size: 3793 2827
EarI CTCTTCn'nnn_
Cuts at: 2728 2794 3356 4643 4853 2728
Size: 66 562 1287 210 4495
Fragments arranged by size:
4495 1287 562 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 188 475 1672 2415 4828 5138 5284 6430 188
Size: 287 1197 743 2413 310 146 1146 378
Fragments arranged by size:
2413 1197 1146 743 378 310 287 146
EcoICRI GAG'CTC
Cuts at: 3632 3632
Size: 6620
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 553 1708 1783 1834 2017 2606 2611 2712 2731
Size: 1155 75 51 183 589 5 101 19
Cuts at: 2731 2833 2950 3368 4445 4877 5624 5892 553
Size: 102 117 418 1077 432 747 268 1281
Fragments arranged by size:
1281 1155 1077 747 589 432 418 268
183 117 102 101 75 51 19 5
EcoNI CCTnn'n_nnAGG
Cuts at: 1948 1948
Size: 6620
EcoO109I rG'GnC_Cy
Cuts at: 348 380 786 957 996 1038 1992 2523 2524
Size: 32 406 171 39 42 954 531 1
Cuts at: 2524 3759 348
Size: 1235 3209
Fragments arranged by size:
3209 1235 954 531 406 171 42 39
32 1
EcoRI G'AATT_C
Cuts at: 3624 3624
Size: 6620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4639 4639
Size: 6620
FspI TGC'GCA
Cuts at: 4401 4401
Size: 6620
HaeII r_GCGC'y
Cuts at: 557 2253 3122 4054 4302 5324 6303 557
Size: 1696 869 932 248 1022 979 874
Fragments arranged by size:
1696 1022 979 932 874 869 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 988 1020 1301 1333 2521 2553 2978 3010 3079
Size: 32 281 32 1188 32 425 32 69
Cuts at: 3079 3111 3568 3600 4462 4492 4494 4524 988
Size: 32 457 32 862 30 2 30 3084
Fragments arranged by size:
3084 1188 862 457 425 281 69 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2151 2184 3659 2151
Size: 33 1475 5112
Fragments arranged by size:
5112 1475 33
HindIII A'AGCT_T
Cuts at: 873 3182 873
Size: 2309 4311
HpaI GTT'AAC
Cuts at: 2151 2151
Size: 6620
KasI G'GCGC_C
Cuts at: 4050 4298 4050
Size: 248 6372
KpnI G_GTAC'C
Cuts at: 3640 3640
Size: 6620
MluI A'CGCG_T
Cuts at: 6053 6053
Size: 6620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 713 1121 1397 2130 2672 5291 5475 713
Size: 408 276 733 542 2619 184 1858
Fragments arranged by size:
2619 1858 733 542 408 276 184
MscI TGG'CCA
Cuts at: 112 1396 1738 1948 4381 112
Size: 1284 342 210 2433 2351
Fragments arranged by size:
2433 2351 1284 342 210
MslI CAynn'nnrTG
Cuts at: 1462 1527 1580 1753 2969 4609 4736 1462
Size: 65 53 173 1216 1640 127 3346
Fragments arranged by size:
3346 1640 1216 173 127 65 53
MspA1I CmG'CkG
Cuts at: 1761 3731 4045 4405 5418 5663 1761
Size: 1970 314 360 1013 245 2718
Fragments arranged by size:
2718 1970 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4801 4801
Size: 6620
NarI GG'CG_CC
Cuts at: 4051 4299 4051
Size: 248 6372
NcoI C'CATG_G
Cuts at: 107 626 107
Size: 519 6101
NgoMIV G'CCGG_C
Cuts at: 4799 4799
Size: 6620
NsiI A_TGCA'T
Cuts at: 946 946
Size: 6620
NspI r_CATG'y
Cuts at: 711 840 3673 4173 5080 711
Size: 129 2833 500 907 2251
Fragments arranged by size:
2833 2251 907 500 129
PasI CC'CwG_GG
Cuts at: 131 888 2570 3216 131
Size: 757 1682 646 3535
Fragments arranged by size:
3535 1682 757 646
PciI A'CATG_T
Cuts at: 836 5076 836
Size: 4240 2380
PflMI CCAn_nnn'nTGG
Cuts at: 1251 1944 1251
Size: 693 5927
PfoI T'CCnGG_A
Cuts at: 1325 1325
Size: 6620
PmeI GTTT'AAAC
Cuts at: 3619 3619
Size: 6620
PmlI CAC'GTG
Cuts at: 1913 2336 1913
Size: 423 6197
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3481 3513 5759 5791 3481
Size: 32 2246 32 4310
Fragments arranged by size:
4310 2246 32 32
PpuMI rG'GwC_Cy
Cuts at: 348 380 786 957 996 1992 348
Size: 32 406 171 39 996 4976
Fragments arranged by size:
4976 996 406 171 39 32
PshAI GACnn'nnGTC
Cuts at: 2470 2470
Size: 6620
PsiI TTA'TAA
Cuts at: 806 806
Size: 6620
PspOMI G'GGCC_C
Cuts at: 2523 2523
Size: 6620
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2626 2658 2626
Size: 32 6588
PstI C_TGCA'G
Cuts at: 494 704 3410 3667 4352 494
Size: 210 2706 257 685 2762
Fragments arranged by size:
2762 2706 685 257 210
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6620
PvuII CAG'CTG
Cuts at: 1761 4045 4405 1761
Size: 2284 360 3976
Fragments arranged by size:
3976 2284 360
RsrII CG'GwC_CG
Cuts at: 4815 4815
Size: 6620
SacI G_AGCT'C
Cuts at: 3634 3634
Size: 6620
SalI G'TCGA_C
Cuts at: 3657 3657
Size: 6620
SanDI GG'GwC_CC
Cuts at: 380 957 380
Size: 577 6043
SapI GCTCTTCn'nnn_
Cuts at: 4643 4853 4643
Size: 210 6410
SbfI CC_TGCA'GG
Cuts at: 494 3410 3667 494
Size: 2916 257 3447
Fragments arranged by size:
3447 2916 257
ScaI AGT'ACT
Cuts at: 6137 6137
Size: 6620
SexAI A'CCwGG_T
Cuts at: 1730 1730
Size: 6620
SfcI C'TryA_G
Cuts at: 33 490 700 720 2796 3381 3406 3663 4348
Size: 457 210 20 2076 585 25 257 685
Cuts at: 4348 5341 5532 33
Size: 993 191 1121
Fragments arranged by size:
2076 1121 993 685 585 457 257 210
191 25 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 1983 1983
Size: 6620
SfoI GGC'GCC
Cuts at: 4052 4300 4052
Size: 248 6372
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6620
SmaI CCC'GGG
Cuts at: 355 1990 2046 3642 355
Size: 1635 56 1596 3333
Fragments arranged by size:
3333 1635 1596 56
SmlI C'TyrA_G
Cuts at: 781 819 2411 3775 4690 5182 5444 781
Size: 38 1592 1364 915 492 262 1957
Fragments arranged by size:
1957 1592 1364 915 492 262 38
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6620
SphI G_CATG'C
Cuts at: 3673 4173 3673
Size: 500 6120
StuI AGG'CCT
Cuts at: 1954 2419 1954
Size: 465 6155
StyI C'CwwG_G
Cuts at: 107 344 518 626 1949 2861 2899 3339 3356
Size: 237 174 108 1323 912 38 440 17
Cuts at: 3356 3754 4108 107
Size: 398 354 2619
Fragments arranged by size:
2619 1323 912 440 398 354 237 174
108 38 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 655 730 763 1348 2020 2712 3229 4203 4336
Size: 75 33 585 672 692 517 974 133
Cuts at: 4336 4795 5004 655
Size: 459 209 2271
Fragments arranged by size:
2271 974 692 672 585 517 459 209
133 75 33
TatI w'GTAC_w
Cuts at: 839 3527 6135 839
Size: 2688 2608 1324
Fragments arranged by size:
2688 2608 1324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 1771 1803 2320 2352 6137 6169 424
Size: 32 1315 32 517 32 3785 32 875
Fragments arranged by size:
3785 1315 875 517 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 535 2077 2653 4417 535
Size: 1542 576 1764 2738
Fragments arranged by size:
2738 1764 1542 576
XbaI T'CTAG_A
Cuts at: 3651 3651
Size: 6620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 128 974 1656 1758 1881 2107 3129 3252 4115
Size: 846 682 102 123 226 1022 123 863
Cuts at: 4115 128
Size: 2633
Fragments arranged by size:
2633 1022 863 846 682 226 123 123
102
XhoI C'TCGA_G
Cuts at: 2411 2411
Size: 6620
XmaI C'CCGG_G
Cuts at: 353 1988 2044 3640 353
Size: 1635 56 1596 3333
Fragments arranged by size:
3333 1635 1596 56
XmnI GAAnn'nnTTC
Cuts at: 772 772
Size: 6620
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AloI AscI BaeI BcgI BmtI BsiWI BssHII
BstZ17I Bsu36I ClaI EcoRV FseI FspAI MfeI NdeI
NheI NotI NruI PacI PspXI SacII SgrAI SnaBI
SrfI SspI SwaI ZraI