Restriction map for
pF1KA0766
(Circular) (Six-base) MAPSORT of: pF1KA0766.seq Check: 5101 from: 1 to: 4934
pF1KA0766 4934 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 269 269
Size: 4934
AccI GT'mk_AC
Cuts at: 143 470 1972 143
Size: 327 1502 3105
Fragments arranged by size:
3105 1502 327
Acc65I G'GTAC_C
Cuts at: 1950 1950
Size: 4934
AclI AA'CG_TT
Cuts at: 4774 4774
Size: 4934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 1621 2759 3191 3938 4206 407
Size: 1214 1138 432 747 268 1135
Fragments arranged by size:
1214 1138 1135 747 432 268
AflIII A'CryG_T
Cuts at: 2286 3390 4367 2286
Size: 1104 977 2853
Fragments arranged by size:
2853 1104 977
AgeI A'CCGG_T
Cuts at: 3322 3322
Size: 4934
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1709 1709
Size: 4934
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1528 1560 1528
Size: 32 4902
AlwNI CAG_nnn'CTG
Cuts at: 2239 3806 2239
Size: 1567 3367
ApaI G_GGCC'C
Cuts at: 219 219
Size: 4934
ApaLI G'TGCA_C
Cuts at: 3704 3704
Size: 4934
ApoI r'AATT_y
Cuts at: 1057 1365 1516 1938 4287 4543 1057
Size: 308 151 422 2349 256 1448
Fragments arranged by size:
2349 1448 422 308 256 151
AseI AT'TA_AT
Cuts at: 20 20
Size: 4934
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
AvaI C'yCGr_G
Cuts at: 166 412 508 1954 166
Size: 246 96 1446 3146
Fragments arranged by size:
3146 1446 246 96
BamHI G'GATC_C
Cuts at: 566 1959 566
Size: 1393 3541
BanI G'GyrC_C
Cuts at: 272 1950 2364 2612 2647 272
Size: 1678 414 248 35 2559
Fragments arranged by size:
2559 1678 414 248 35
BanII G_rGCy'C
Cuts at: 219 419 973 1886 1948 2978 219
Size: 200 554 913 62 1030 2175
Fragments arranged by size:
2175 1030 913 554 200 62
BbeI G_GCGC'C
Cuts at: 276 2368 2616 276
Size: 2092 248 2594
Fragments arranged by size:
2594 2092 248
BciVI GTATCCnnnnn_n'
Cuts at: 2832 3593 4934 2832
Size: 761 1341 2832
Fragments arranged by size:
2832 1341 761
BclI T'GATC_A
Cuts at: 2454 2454
Size: 4934
BglII A'GATC_T
Cuts at: 726 2449 4486 726
Size: 1723 2037 1174
Fragments arranged by size:
2037 1723 1174
BlpI GC'TnA_GC
Cuts at: 287 814 2046 287
Size: 527 1232 3175
Fragments arranged by size:
3175 1232 527
Bme1580I G_kGCm'C
Cuts at: 219 2559 2652 3708 219
Size: 2340 93 1056 1445
Fragments arranged by size:
2340 1445 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2146 2227 2562 4706 2146
Size: 81 335 2144 2374
Fragments arranged by size:
2374 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 416 3301 416
Size: 2885 2049
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 530 530
Size: 4934
Bpu10I CC'TnA_GC
Cuts at: 571 1132 571
Size: 561 4373
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2110 2989 3481 3779 2110
Size: 879 492 298 3265
Fragments arranged by size:
3265 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4638 4638
Size: 4934
BsaAI yAC'GTr
Cuts at: 2287 2287
Size: 4934
BsaBI GATnn'nnATC
Cuts at: 6 989 1484 1841 2448 4485 6
Size: 983 495 357 607 2037 455
Fragments arranged by size:
2037 983 607 495 455 357
BsaHI Gr'CG_yC
Cuts at: 118 266 273 2365 2613 3315 4811 118
Size: 148 7 2092 248 702 1496 241
Fragments arranged by size:
2092 1496 702 248 241 148 7
BsaWI w'CCGG_w
Cuts at: 201 2123 2644 3322 3596 3743 4154 201
Size: 1922 521 678 274 147 411 981
Fragments arranged by size:
1922 981 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 176 449 2359 3117 3694 4190 176
Size: 273 1910 758 577 496 920
Fragments arranged by size:
1910 920 758 577 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 425 425
Size: 4934
BsiEI CG_ry'CG
Cuts at: 104 2476 2522 3730 104
Size: 2372 46 1208 1308
Fragments arranged by size:
2372 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 973 1948 2726 2916 3708 293
Size: 680 975 778 190 792 1519
Fragments arranged by size:
1519 975 792 778 680 190
BsmBI CGTCTCn'nnnn_
Cuts at: 891 1510 891
Size: 619 4315
Bsp1286I G_dGCh'C
Cuts at: 219 293 419 973 1886 1948 2559 2652 2726
Size: 74 126 554 913 62 611 93 74
Cuts at: 2726 2916 2978 3708 219
Size: 190 62 730 1445
Fragments arranged by size:
1445 913 730 611 554 190 126 93
74 74 62 62
BspEI T'CCGG_A
Cuts at: 201 4154 201
Size: 3953 981
BspHI T'CATG_A
Cuts at: 4110 4110
Size: 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1984 2198 2500 2881 1984
Size: 214 302 381 4037
Fragments arranged by size:
4037 381 302 214
BsrBI CCG'CTC
Cuts at: 50 306 3226 3280 4764 4929 50
Size: 256 2920 54 1484 165 55
Fragments arranged by size:
2920 1484 256 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2846 2846
Size: 4934
BsrFI r'CCGG_y
Cuts at: 2932 3113 3322 2932
Size: 181 209 4544
Fragments arranged by size:
4544 209 181
BssHII G'CGCG_C
Cuts at: 327 327
Size: 4934
BssSI C'ACGA_G
Cuts at: 401 3205 3563 401
Size: 2804 358 1772
Fragments arranged by size:
2804 1772 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2311 2311
Size: 4934
BstBI TT'CG_AA
Cuts at: 1352 3295 1352
Size: 1943 2991
BstYI r'GATC_y
Cuts at: 1 566 726 1959 2426 2449 2784 3030 4042
Size: 565 160 1233 467 23 335 246 1012
Cuts at: 4042 4128 4140 4486 1
Size: 86 12 346 449
Fragments arranged by size:
1233 1012 565 467 449 346 335 246
160 86 23 12
BstZ17I GTA'TAC
Cuts at: 471 471
Size: 4934
Bsu36I CC'TnA_GG
Cuts at: 1540 1540
Size: 4934
BtgI C'CryG_G
Cuts at: 368 693 368
Size: 325 4609
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 2884 3065 3228 224
Size: 2660 181 163 1930
Fragments arranged by size:
2660 1930 181 163
BtsI GCAGTG_nn'
Cuts at: 2177 2177
Size: 4934
DraI TTT'AAA
Cuts at: 624 1151 1590 1933 624
Size: 527 439 343 3625
Fragments arranged by size:
3625 527 439 343
DrdI GACnn_nn'nnGTC
Cuts at: 2640 3498 2640
Size: 858 4076
EaeI y'GGCC_r
Cuts at: 2519 2693 3084 3111 4456 2519
Size: 174 391 27 1345 2997
Fragments arranged by size:
2997 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2519 2519
Size: 4934
EarI CTCTTCn'nnn_
Cuts at: 383 2957 3167 383
Size: 2574 210 2150
Fragments arranged by size:
2574 2150 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 331 3142 3452 3598 4744 331
Size: 2811 310 146 1146 521
Fragments arranged by size:
2811 1146 521 310 146
EcoICRI GAG'CTC
Cuts at: 971 1946 971
Size: 975 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 530 1621 2759 3191 3938 4206 407
Size: 123 1091 1138 432 747 268 1135
Fragments arranged by size:
1138 1135 1091 747 432 268 123
EcoO109I rG'GnC_Cy
Cuts at: 215 216 703 1536 2073 215
Size: 1 487 833 537 3076
Fragments arranged by size:
3076 833 537 487 1
EcoRI G'AATT_C
Cuts at: 1938 1938
Size: 4934
EcoRV GAT'ATC
Cuts at: 552 552
Size: 4934
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 745 2953 745
Size: 2208 2726
FspI TGC'GCA
Cuts at: 2715 2715
Size: 4934
HaeII r_GCGC'y
Cuts at: 276 2368 2616 3638 4617 276
Size: 2092 248 1022 979 593
Fragments arranged by size:
2092 1022 979 593 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1488 1520 2776 2806 2808 2838 1488
Size: 32 1256 30 2 30 3584
Fragments arranged by size:
3584 1256 32 30 30 2
HincII GTy'rAC
Cuts at: 144 537 1973 144
Size: 393 1436 3105
Fragments arranged by size:
3105 1436 393
KasI G'GCGC_C
Cuts at: 272 2364 2612 272
Size: 2092 248 2594
Fragments arranged by size:
2594 2092 248
KpnI G_GTAC'C
Cuts at: 1954 1954
Size: 4934
MfeI C'AATT_G
Cuts at: 1125 1125
Size: 4934
MluI A'CGCG_T
Cuts at: 4367 4367
Size: 4934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 692 813 904 3605 3789 692
Size: 121 91 2701 184 1837
Fragments arranged by size:
2701 1837 184 121 91
MscI TGG'CCA
Cuts at: 2695 2695
Size: 4934
MslI CAynn'nnrTG
Cuts at: 112 484 761 2923 3050 112
Size: 372 277 2162 127 1996
Fragments arranged by size:
2162 1996 372 277 127
MspA1I CmG'CkG
Cuts at: 695 2045 2359 2719 3732 3977 695
Size: 1350 314 360 1013 245 1652
Fragments arranged by size:
1652 1350 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3115 3115
Size: 4934
NarI GG'CG_CC
Cuts at: 273 2365 2613 273
Size: 2092 248 2594
Fragments arranged by size:
2594 2092 248
NgoMIV G'CCGG_C
Cuts at: 3113 3113
Size: 4934
NspI r_CATG'y
Cuts at: 517 1987 2487 3394 517
Size: 1470 500 907 2057
Fragments arranged by size:
2057 1470 907 500
PciI A'CATG_T
Cuts at: 3390 3390
Size: 4934
PflMI CCAn_nnn'nTGG
Cuts at: 480 923 480
Size: 443 4491
PmeI GTTT'AAAC
Cuts at: 1933 1933
Size: 4934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4902
PpuMI rG'GwC_Cy
Cuts at: 1536 1536
Size: 4934
PspOMI G'GGCC_C
Cuts at: 215 215
Size: 4934
PstI C_TGCA'G
Cuts at: 803 1981 2666 803
Size: 1178 685 3071
Fragments arranged by size:
3071 1178 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4934
PvuII CAG'CTG
Cuts at: 2359 2719 2359
Size: 360 4574
RsrII CG'GwC_CG
Cuts at: 3129 3129
Size: 4934
SacI G_AGCT'C
Cuts at: 973 1948 973
Size: 975 3959
SacII CC_GC'GG
Cuts at: 696 696
Size: 4934
SalI G'TCGA_C
Cuts at: 142 1971 142
Size: 1829 3105
SapI GCTCTTCn'nnn_
Cuts at: 2957 3167 2957
Size: 210 4724
SbfI CC_TGCA'GG
Cuts at: 1981 1981
Size: 4934
ScaI AGT'ACT
Cuts at: 4451 4451
Size: 4934
SfcI C'TryA_G
Cuts at: 33 799 1977 2662 3655 3846 33
Size: 766 1178 685 993 191 1121
Fragments arranged by size:
1178 1121 993 766 685 191
SfoI GGC'GCC
Cuts at: 274 2366 2614 274
Size: 2092 248 2594
Fragments arranged by size:
2594 2092 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
SmaI CCC'GGG
Cuts at: 1956 1956
Size: 4934
SmlI C'TyrA_G
Cuts at: 2089 3004 3496 3758 2089
Size: 915 492 262 3265
Fragments arranged by size:
3265 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4934
SphI G_CATG'C
Cuts at: 1987 2487 1987
Size: 500 4434
SspI AAT'ATT
Cuts at: 1160 1469 1160
Size: 309 4625
StuI AGG'CCT
Cuts at: 410 410
Size: 4934
StyI C'CwwG_G
Cuts at: 641 2068 2422 641
Size: 1427 354 3153
Fragments arranged by size:
3153 1427 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2517 2650 3109 3318 2517
Size: 133 459 209 4133
Fragments arranged by size:
4133 459 209 133
TatI w'GTAC_w
Cuts at: 1345 4449 1345
Size: 3104 1830
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 852 884 4451 4483 852
Size: 32 3567 32 1303
Fragments arranged by size:
3567 1303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1115 2731 1115
Size: 1616 3318
XbaI T'CTAG_A
Cuts at: 1965 1965
Size: 4934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1875 2429 1875
Size: 554 4380
XmaI C'CCGG_G
Cuts at: 1954 1954
Size: 4934
XmnI GAAnn'nnTTC
Cuts at: 728 1277 728
Size: 549 4385
ZraI GAC'GTC
Cuts at: 267 267
Size: 4934
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BsrGI BstEII BstXI ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI