Restriction map for
pF1KA0772
(Circular) (Six-base) MAPSORT of: pF1KA0772.seq Check: 846 from: 1 to: 4517
pF1KA0772 4517 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 787 787
Size: 4517
AccI GT'mk_AC
Cuts at: 1555 1555
Size: 4517
Acc65I G'GTAC_C
Cuts at: 1533 1533
Size: 4517
AclI AA'CG_TT
Cuts at: 4357 4357
Size: 4517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 331 2342 2774 3127 3789 276
Size: 55 2011 432 353 662 1004
Fragments arranged by size:
2011 1004 662 432 353 55
AflIII A'CryG_T
Cuts at: 410 1869 3669 3950 410
Size: 1459 1800 281 977
Fragments arranged by size:
1800 1459 977 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1171 1171
Size: 4517
AlwNI CAG_nnn'CTG
Cuts at: 450 1822 3260 450
Size: 1372 1438 1707
Fragments arranged by size:
1707 1438 1372
ApaLI G'TGCA_C
Cuts at: 3355 3355
Size: 4517
ApoI r'AATT_y
Cuts at: 124 239 1485 1521 3870 4126 124
Size: 115 1246 36 2349 256 515
Fragments arranged by size:
2349 1246 515 256 115 36
AseI AT'TA_AT
Cuts at: 20 556 20
Size: 536 3981
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
AvaI C'yCGr_G
Cuts at: 716 1537 716
Size: 821 3696
BamHI G'GATC_C
Cuts at: 1542 1542
Size: 4517
BanI G'GyrC_C
Cuts at: 721 1533 1947 2195 2230 721
Size: 812 414 248 35 3008
Fragments arranged by size:
3008 812 414 248 35
BanII G_rGCy'C
Cuts at: 716 1531 2561 716
Size: 815 1030 2672
Fragments arranged by size:
2672 1030 815
BbeI G_GCGC'C
Cuts at: 1951 2199 1951
Size: 248 4269
BbsI GAAGACnn'nnnn_
Cuts at: 1079 1261 1079
Size: 182 4335
BciVI GTATCCnnnnn_n'
Cuts at: 2415 3471 4517 2415
Size: 1056 1046 2415
Fragments arranged by size:
2415 1056 1046
BclI T'GATC_A
Cuts at: 2037 2037
Size: 4517
BglII A'GATC_T
Cuts at: 2032 4069 2032
Size: 2037 2480
BlpI GC'TnA_GC
Cuts at: 1629 1629
Size: 4517
Bme1580I G_kGCm'C
Cuts at: 2142 2235 3359 2142
Size: 93 1124 3300
Fragments arranged by size:
3300 1124 93
BmgBI CAC'GTC
Cuts at: 1098 1110 1098
Size: 12 4505
BmrI ACTGGGnnnn_n'
Cuts at: 221 495 1451 1729 1810 2145 4289 221
Size: 274 956 278 81 335 2144 449
Fragments arranged by size:
2144 956 449 335 278 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 240 242 2884 240
Size: 2 2642 1873
Fragments arranged by size:
2642 1873 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 472 754 1182 1258 1369 349
Size: 123 282 428 76 111 3497
Fragments arranged by size:
3497 428 282 123 111 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 667 1693 2572 3286 3584 212
Size: 455 1026 879 714 298 1145
Fragments arranged by size:
1145 1026 879 714 455 298
BsaI GGTCTCn'nnnn_
Cuts at: 643 1120 4221 643
Size: 477 3101 939
Fragments arranged by size:
3101 939 477
BsaAI yAC'GTr
Cuts at: 411 1870 411
Size: 1459 3058
BsaBI GATnn'nnATC
Cuts at: 6 352 585 2031 4068 6
Size: 346 233 1446 2037 455
Fragments arranged by size:
2037 1446 455 346 233
BsaHI Gr'CG_yC
Cuts at: 1948 2196 2898 4394 1948
Size: 248 702 1496 2071
Fragments arranged by size:
2071 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1706 2227 2905 3316 3463 3737 1706
Size: 521 678 411 147 274 2486
Fragments arranged by size:
2486 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 397 415 427 445 1384 1414 397
Size: 18 12 18 939 30 3500
Fragments arranged by size:
3500 939 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1050 1366 1405 1050
Size: 316 39 4162
Fragments arranged by size:
4162 316 39
BseYI C'CCAG_C
Cuts at: 443 1942 2700 3365 3773 443
Size: 1499 758 665 408 1187
Fragments arranged by size:
1499 1187 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1153
Size: 4517
BsiEI CG_ry'CG
Cuts at: 104 2059 2105 3335 104
Size: 1955 46 1230 1286
Fragments arranged by size:
1955 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 410 1531 2309 2499 3359 410
Size: 1121 778 190 860 1568
Fragments arranged by size:
1568 1121 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 410 716 1531 2142 2235 2309 2499 2561 3359
Size: 306 815 611 93 74 190 62 798
Cuts at: 3359 410
Size: 1568
Fragments arranged by size:
1568 815 798 611 306 190 93 74
62
BspHI T'CATG_A
Cuts at: 2949 2949
Size: 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 787 1125 1567 1781 2083 2464 787
Size: 338 442 214 302 381 2840
Fragments arranged by size:
2840 442 381 338 302 214
BsrBI CCG'CTC
Cuts at: 50 1344 1380 1482 2809 2863 4347 4512 50
Size: 1294 36 102 1327 54 1484 165 55
Fragments arranged by size:
1484 1327 1294 165 102 55 54 36
BsrDI GCAATG_nn'
Cuts at: 2429 2429
Size: 4517
BsrFI r'CCGG_y
Cuts at: 264 514 2515 2696 264
Size: 250 2001 181 2085
Fragments arranged by size:
2085 2001 250 181
BssSI C'ACGA_G
Cuts at: 2788 3496 2788
Size: 708 3809
BstAPI GCAn_nnn'nTGC
Cuts at: 1894 1894
Size: 4517
BstBI TT'CG_AA
Cuts at: 2878 2878
Size: 4517
BstEII G'GTnAC_C
Cuts at: 1042 1042
Size: 4517
BstXI CCAn_nnnn'nTGG
Cuts at: 732 856 732
Size: 124 4393
BstYI r'GATC_y
Cuts at: 1 999 1320 1542 2009 2032 2367 2613 2919
Size: 998 321 222 467 23 335 246 306
Cuts at: 2919 2931 3017 4069 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 998 467 449 335 321 306 246
222 86 23 12
BtgI C'CryG_G
Cuts at: 660 1268 1296 660
Size: 608 28 3881
Fragments arranged by size:
3881 608 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 242 2467 2648 2811 242
Size: 2225 181 163 1948
Fragments arranged by size:
2225 1948 181 163
BtsI GCAGTG_nn'
Cuts at: 1760 1760
Size: 4517
DraI TTT'AAA
Cuts at: 845 882 1516 845
Size: 37 634 3846
Fragments arranged by size:
3846 634 37
DraIII CAC_nnn'GTG
Cuts at: 918 918
Size: 4517
DrdI GACnn_nn'nnGTC
Cuts at: 2223 3567 2223
Size: 1344 3173
EaeI y'GGCC_r
Cuts at: 1325 2102 2276 2667 2694 4039 1325
Size: 777 174 391 27 1345 1803
Fragments arranged by size:
1803 1345 777 391 174 27
EagI C'GGCC_G
Cuts at: 2102 2102
Size: 4517
EarI CTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 724 2725 3467 3613 4327 724
Size: 2001 742 146 714 914
Fragments arranged by size:
2001 914 742 714 146
EcoICRI GAG'CTC
Cuts at: 1529 1529
Size: 4517
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 276 331 349 472 754 1182 1258 1369 2342
Size: 55 18 123 282 428 76 111 973
Cuts at: 2342 2774 3127 3789 276
Size: 432 353 662 1004
Fragments arranged by size:
1004 973 662 432 428 353 282 123
111 76 55 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1054 1054
Size: 4517
EcoO109I rG'GnC_Cy
Cuts at: 221 428 1445 1656 221
Size: 207 1017 211 3082
Fragments arranged by size:
3082 1017 211 207
EcoRI G'AATT_C
Cuts at: 124 239 1521 124
Size: 115 1282 3120
Fragments arranged by size:
3120 1282 115
EcoRV GAT'ATC
Cuts at: 1506 1506
Size: 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 972 2536 972
Size: 1564 2953
FspI TGC'GCA
Cuts at: 2298 2298
Size: 4517
HaeII r_GCGC'y
Cuts at: 1951 2199 3429 4200 1951
Size: 248 1230 771 2268
Fragments arranged by size:
2268 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 710 742 1060 1092 2359 2389 2391 2421 710
Size: 32 318 32 1267 30 2 30 2806
Fragments arranged by size:
2806 1267 318 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1556 1556
Size: 4517
KasI G'GCGC_C
Cuts at: 1947 2195 1947
Size: 248 4269
KpnI G_GTAC'C
Cuts at: 1537 1537
Size: 4517
MluI A'CGCG_T
Cuts at: 3950 3950
Size: 4517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1517 3276 3460 1517
Size: 1759 184 2574
Fragments arranged by size:
2574 1759 184
MscI TGG'CCA
Cuts at: 1327 2278 1327
Size: 951 3566
MslI CAynn'nnrTG
Cuts at: 166 866 2506 2633 166
Size: 700 1640 127 2050
Fragments arranged by size:
2050 1640 700 127
MspA1I CmG'CkG
Cuts at: 387 1298 1628 1942 2302 3086 3331 387
Size: 911 330 314 360 784 245 1573
Fragments arranged by size:
1573 911 784 360 330 314 245
NaeI GCC'GGC
Cuts at: 266 2698 266
Size: 2432 2085
NarI GG'CG_CC
Cuts at: 1948 2196 1948
Size: 248 4269
NcoI C'CATG_G
Cuts at: 660 660
Size: 4517
NgoMIV G'CCGG_C
Cuts at: 264 2696 264
Size: 2432 2085
NspI r_CATG'y
Cuts at: 1570 2070 3673 1570
Size: 500 1603 2414
Fragments arranged by size:
2414 1603 500
PciI A'CATG_T
Cuts at: 3669 3669
Size: 4517
PflMI CCAn_nnn'nTGG
Cuts at: 450 450
Size: 4517
PfoI T'CCnGG_A
Cuts at: 1078 1078
Size: 4517
PmeI GTTT'AAAC
Cuts at: 1516 1516
Size: 4517
PmlI CAC'GTG
Cuts at: 411 411
Size: 4517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2963 2995 2963
Size: 32 4485
PpuMI rG'GwC_Cy
Cuts at: 1445 1445
Size: 4517
PstI C_TGCA'G
Cuts at: 1564 2249 1564
Size: 685 3832
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4517
PvuII CAG'CTG
Cuts at: 1942 2302 1942
Size: 360 4157
RsrII CG'GwC_CG
Cuts at: 2712 2712
Size: 4517
SacI G_AGCT'C
Cuts at: 1531 1531
Size: 4517
SacII CC_GC'GG
Cuts at: 1299 1299
Size: 4517
SalI G'TCGA_C
Cuts at: 1554 1554
Size: 4517
SanDI GG'GwC_CC
Cuts at: 1445 1445
Size: 4517
SapI GCTCTTCn'nnn_
Cuts at: 2540 2750 2540
Size: 210 4307
SbfI CC_TGCA'GG
Cuts at: 1564 1564
Size: 4517
ScaI AGT'ACT
Cuts at: 4034 4034
Size: 4517
SfcI C'TryA_G
Cuts at: 33 1560 2245 3213 3404 33
Size: 1527 685 968 191 1146
Fragments arranged by size:
1527 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1949 2197 1949
Size: 248 4269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4517
SmaI CCC'GGG
Cuts at: 1539 1539
Size: 4517
SmlI C'TyrA_G
Cuts at: 227 682 1672 2587 3301 3563 227
Size: 455 990 915 714 262 1181
Fragments arranged by size:
1181 990 915 714 455 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4517
SphI G_CATG'C
Cuts at: 1570 2070 1570
Size: 500 4017
StyI C'CwwG_G
Cuts at: 660 1385 1651 2005 660
Size: 725 266 354 3172
Fragments arranged by size:
3172 725 354 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1281 2100 2233 2692 2901 1281
Size: 819 133 459 209 2897
Fragments arranged by size:
2897 819 459 209 133
TatI w'GTAC_w
Cuts at: 398 4032 398
Size: 3634 883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 973 1005 1384 1416 4034 4066 415
Size: 32 526 32 379 32 2618 32 866
Fragments arranged by size:
2618 866 526 379 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2314 2314
Size: 4517
XbaI T'CTAG_A
Cuts at: 1548 1548
Size: 4517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2012 2012
Size: 4517
XmaI C'CCGG_G
Cuts at: 1537 1537
Size: 4517
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI