Restriction map for
pF1KA0783
(Circular) (Six-base) MAPSORT of: pF1KA0783.seq Check: 5643 from: 1 to: 5777
pF1KA0783 5777 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 123 123
Size: 5777
AccI GT'mk_AC
Cuts at: 2815 2815
Size: 5777
Acc65I G'GTAC_C
Cuts at: 2793 2793
Size: 5777
AclI AA'CG_TT
Cuts at: 5617 5617
Size: 5777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 311 1714 1811 2720 2753 3602 4034 4387
Size: 66 1403 97 909 33 849 432 353
Cuts at: 4387 5049 245
Size: 662 973
Fragments arranged by size:
1403 973 909 849 662 432 353 97
66 33
AflIII A'CryG_T
Cuts at: 3129 4929 5210 3129
Size: 1800 281 3696
Fragments arranged by size:
3696 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1509 1757 1509
Size: 248 5529
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1301 1333 1301
Size: 32 5745
AlwNI CAG_nnn'CTG
Cuts at: 223 529 1201 1306 3082 4520 223
Size: 306 672 105 1776 1438 1480
Fragments arranged by size:
1776 1480 1438 672 306 105
ApaLI G'TGCA_C
Cuts at: 4615 4615
Size: 5777
ApoI r'AATT_y
Cuts at: 354 1286 2003 2533 2781 5130 5386 354
Size: 932 717 530 248 2349 256 745
Fragments arranged by size:
2349 932 745 717 530 256 248
AseI AT'TA_AT
Cuts at: 20 394 20
Size: 374 5403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
AvaI C'yCGr_G
Cuts at: 2797 2797
Size: 5777
BamHI G'GATC_C
Cuts at: 2373 2802 2373
Size: 429 5348
BanI G'GyrC_C
Cuts at: 1417 2793 3207 3455 3490 1417
Size: 1376 414 248 35 3704
Fragments arranged by size:
3704 1376 414 248 35
BanII G_rGCy'C
Cuts at: 1045 2791 3821 1045
Size: 1746 1030 3001
Fragments arranged by size:
3001 1746 1030
BbeI G_GCGC'C
Cuts at: 3211 3459 3211
Size: 248 5529
BbsI GAAGACnn'nnnn_
Cuts at: 857 1448 2667 857
Size: 591 1219 3967
Fragments arranged by size:
3967 1219 591
BciVI GTATCCnnnnn_n'
Cuts at: 3675 4731 5777 3675
Size: 1056 1046 3675
Fragments arranged by size:
3675 1056 1046
BclI T'GATC_A
Cuts at: 2313 3297 2313
Size: 984 4793
BglII A'GATC_T
Cuts at: 778 3292 5329 778
Size: 2514 2037 1226
Fragments arranged by size:
2514 2037 1226
BlpI GC'TnA_GC
Cuts at: 2889 2889
Size: 5777
Bme1580I G_kGCm'C
Cuts at: 1335 3402 3495 4619 1335
Size: 2067 93 1124 2493
Fragments arranged by size:
2493 2067 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1472 2989 3070 3405 5549 1472
Size: 1517 81 335 2144 1700
Fragments arranged by size:
2144 1700 1517 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1197 4144 1197
Size: 2947 2830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1059 1367 2759 1059
Size: 308 1392 4077
Fragments arranged by size:
4077 1392 308
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 2953 3832 4546 4844 1042
Size: 1911 879 714 298 1975
Fragments arranged by size:
1975 1911 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 5481 5481
Size: 5777
BsaAI yAC'GTr
Cuts at: 3130 3130
Size: 5777
BsaBI GATnn'nnATC
Cuts at: 6 783 878 2318 3291 5328 6
Size: 777 95 1440 973 2037 455
Fragments arranged by size:
2037 1440 973 777 455 95
BsaHI Gr'CG_yC
Cuts at: 3208 3456 4158 5654 3208
Size: 248 702 1496 3331
Fragments arranged by size:
3331 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2966 3487 4165 4576 4723 4997 2966
Size: 521 678 411 147 274 3746
Fragments arranged by size:
3746 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 561 591 561
Size: 30 5747
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 141 2369 141
Size: 2228 3549
BseYI C'CCAG_C
Cuts at: 3202 3960 4625 5033 3202
Size: 758 665 408 3946
Fragments arranged by size:
3946 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1515 1515
Size: 5777
BsiEI CG_ry'CG
Cuts at: 104 3319 3365 4595 104
Size: 3215 46 1230 1286
Fragments arranged by size:
3215 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1045 1524 2214 2428 2791 3569 3759 4619 1045
Size: 479 690 214 363 778 190 860 2203
Fragments arranged by size:
2203 860 778 690 479 363 214 190
Bsp1286I G_dGCh'C
Cuts at: 1045 1335 1524 2214 2428 2791 3402 3495 3569
Size: 290 189 690 214 363 611 93 74
Cuts at: 3569 3759 3821 4619 1045
Size: 190 62 798 2203
Fragments arranged by size:
2203 798 690 611 363 290 214 190
189 93 74 62
BspHI T'CATG_A
Cuts at: 4209 4209
Size: 5777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 123 2827 3041 3343 3724 123
Size: 2704 214 302 381 2176
Fragments arranged by size:
2704 2176 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4069 4123 5607 5772 50
Size: 4019 54 1484 165 55
Fragments arranged by size:
4019 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 876 3689 876
Size: 2813 2964
BsrFI r'CCGG_y
Cuts at: 2196 3775 3956 2196
Size: 1579 181 4017
Fragments arranged by size:
4017 1579 181
BssSI C'ACGA_G
Cuts at: 2586 4048 4756 2586
Size: 1462 708 3607
Fragments arranged by size:
3607 1462 708
BstAPI GCAn_nnn'nTGC
Cuts at: 3154 3154
Size: 5777
BstBI TT'CG_AA
Cuts at: 2133 4138 2133
Size: 2005 3772
BstXI CCAn_nnnn'nTGG
Cuts at: 653 653
Size: 5777
BstYI r'GATC_y
Cuts at: 1 778 1572 2373 2802 3269 3292 3627 3873
Size: 777 794 801 429 467 23 335 246
Cuts at: 3873 4179 4191 4277 5329 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 801 794 777 467 449 429 335
306 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2621 2621
Size: 5777
BtgI C'CryG_G
Cuts at: 107 695 2474 107
Size: 588 1779 3410
Fragments arranged by size:
3410 1779 588
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3727 3908 4071 3727
Size: 181 163 5433
Fragments arranged by size:
5433 181 163
BtsI GCAGTG_nn'
Cuts at: 1827 1952 2428 2457 3020 1827
Size: 125 476 29 563 4584
Fragments arranged by size:
4584 563 476 125 29
DraI TTT'AAA
Cuts at: 201 2776 201
Size: 2575 3202
DrdI GACnn_nn'nnGTC
Cuts at: 3483 4827 3483
Size: 1344 4433
EaeI y'GGCC_r
Cuts at: 2471 3362 3536 3927 3954 5299 2471
Size: 891 174 391 27 1345 2949
Fragments arranged by size:
2949 1345 891 391 174 27
EagI C'GGCC_G
Cuts at: 3362 3362
Size: 5777
EarI CTCTTCn'nnn_
Cuts at: 695 916 1021 1553 1621 2273 3800 4010 695
Size: 221 105 532 68 652 1527 210 2462
Fragments arranged by size:
2462 1527 652 532 221 210 105 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1570 3985 4727 4873 5587 1570
Size: 2415 742 146 714 1760
Fragments arranged by size:
2415 1760 742 714 146
EcoICRI GAG'CTC
Cuts at: 1043 2789 1043
Size: 1746 4031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 311 1059 1367 1714 1811 2720 2753 2759
Size: 66 748 308 347 97 909 33 6
Cuts at: 2759 3602 4034 4387 5049 245
Size: 843 432 353 662 973
Fragments arranged by size:
973 909 843 748 662 432 353 347
308 97 66 33 6
EcoO109I rG'GnC_Cy
Cuts at: 2916 2916
Size: 5777
EcoRI G'AATT_C
Cuts at: 2781 2781
Size: 5777
EcoRV GAT'ATC
Cuts at: 1881 1881
Size: 5777
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 364 1555 2083 3796 364
Size: 1191 528 1713 2345
Fragments arranged by size:
2345 1713 1191 528
FspI TGC'GCA
Cuts at: 1993 3558 1993
Size: 1565 4212
FspAI rTGC'GCAy
Cuts at: 1993 1993
Size: 5777
HaeII r_GCGC'y
Cuts at: 3211 3459 4689 5460 3211
Size: 248 1230 771 3528
Fragments arranged by size:
3528 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 676 708 972 1004 1506 1538 2250
Size: 32 541 32 264 32 502 32 712
Cuts at: 2250 2282 3619 3649 3651 3681 103
Size: 32 1337 30 2 30 2199
Fragments arranged by size:
2199 1337 712 541 502 264 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 588 2816 588
Size: 2228 3549
HindIII A'AGCT_T
Cuts at: 2065 2610 2065
Size: 545 5232
HpaI GTT'AAC
Cuts at: 588 588
Size: 5777
KasI G'GCGC_C
Cuts at: 3207 3455 3207
Size: 248 5529
KpnI G_GTAC'C
Cuts at: 2797 2797
Size: 5777
MfeI C'AATT_G
Cuts at: 1128 1128
Size: 5777
MluI A'CGCG_T
Cuts at: 5210 5210
Size: 5777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 186 4536 4720 186
Size: 4350 184 1243
Fragments arranged by size:
4350 1243 184
MscI TGG'CCA
Cuts at: 2473 3538 2473
Size: 1065 4712
MslI CAynn'nnrTG
Cuts at: 3766 3893 3766
Size: 127 5650
MspA1I CmG'CkG
Cuts at: 1552 2888 3202 3562 4346 4591 1552
Size: 1336 314 360 784 245 2738
Fragments arranged by size:
2738 1336 784 360 314 245
NaeI GCC'GGC
Cuts at: 3958 3958
Size: 5777
NarI GG'CG_CC
Cuts at: 3208 3456 3208
Size: 248 5529
NcoI C'CATG_G
Cuts at: 107 695 2474 107
Size: 588 1779 3410
Fragments arranged by size:
3410 1779 588
NgoMIV G'CCGG_C
Cuts at: 3956 3956
Size: 5777
NspI r_CATG'y
Cuts at: 1603 2830 3330 4933 1603
Size: 1227 500 1603 2447
Fragments arranged by size:
2447 1603 1227 500
PacI TTA_AT'TAA
Cuts at: 398 398
Size: 5777
PciI A'CATG_T
Cuts at: 4929 4929
Size: 5777
PfoI T'CCnGG_A
Cuts at: 1344 1344
Size: 5777
PmeI GTTT'AAAC
Cuts at: 2776 2776
Size: 5777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4223 4255 4223
Size: 32 5745
PstI C_TGCA'G
Cuts at: 363 1959 2277 2824 3509 363
Size: 1596 318 547 685 2631
Fragments arranged by size:
2631 1596 685 547 318
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5777
PvuII CAG'CTG
Cuts at: 3202 3562 3202
Size: 360 5417
RsrII CG'GwC_CG
Cuts at: 3972 3972
Size: 5777
SacI G_AGCT'C
Cuts at: 1045 2791 1045
Size: 1746 4031
SalI G'TCGA_C
Cuts at: 2814 2814
Size: 5777
SapI GCTCTTCn'nnn_
Cuts at: 695 3800 4010 695
Size: 3105 210 2462
Fragments arranged by size:
3105 2462 210
SbfI CC_TGCA'GG
Cuts at: 2824 2824
Size: 5777
ScaI AGT'ACT
Cuts at: 374 2279 5294 374
Size: 1905 3015 857
Fragments arranged by size:
3015 1905 857
SfcI C'TryA_G
Cuts at: 33 279 359 623 758 1648 1955 2273 2820
Size: 246 80 264 135 890 307 318 547
Cuts at: 2820 3505 4473 4664 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 890 685 547 318 307 264
246 191 135 80
SfoI GGC'GCC
Cuts at: 3209 3457 3209
Size: 248 5529
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5777
SmaI CCC'GGG
Cuts at: 2799 2799
Size: 5777
SmlI C'TyrA_G
Cuts at: 1021 2932 3847 4561 4823 1021
Size: 1911 915 714 262 1975
Fragments arranged by size:
1975 1911 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1906 67
Size: 1839 3938
SphI G_CATG'C
Cuts at: 2830 3330 2830
Size: 500 5277
SspI AAT'ATT
Cuts at: 300 300
Size: 5777
StuI AGG'CCT
Cuts at: 1780 1780
Size: 5777
StyI C'CwwG_G
Cuts at: 107 695 1219 1421 1789 2216 2474 2693 2911
Size: 588 524 202 368 427 258 219 218
Cuts at: 2911 3265 107
Size: 354 2619
Fragments arranged by size:
2619 588 524 427 368 354 258 219
218 202
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2229 3360 3493 3952 4161 2229
Size: 1131 133 459 209 3845
Fragments arranged by size:
3845 1131 459 209 133
TatI w'GTAC_w
Cuts at: 372 1873 2277 5292 372
Size: 1501 404 3015 857
Fragments arranged by size:
3015 1501 857 404
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 561 593 5294 5326 561
Size: 32 4701 32 1012
Fragments arranged by size:
4701 1012 32 32
Tth111I GACn'n_nGTC
Cuts at: 3574 3574
Size: 5777
XbaI T'CTAG_A
Cuts at: 2808 2808
Size: 5777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3272 3272
Size: 5777
XmaI C'CCGG_G
Cuts at: 2797 2797
Size: 5777
XmnI GAAnn'nnTTC
Cuts at: 1115 2066 2645 1115
Size: 951 579 4247
Fragments arranged by size:
4247 951 579
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PacI PciI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AleI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI FseI NdeI NheI
NotI NruI NsiI PasI PflMI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI