(Circular) (Six-base) MAPSORT of: pF1KA0790.seq Check: 40 from: 1 to: 6854 pF1KA0790 6854 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AatII G_ACGT'C Cuts at: 3530 3530 Size: 6854 AccI GT'mk_AC Cuts at: 2771 3892 2771 Size: 1121 5733 Acc65I G'GTAC_C Cuts at: 3870 3870 Size: 6854 AclI AA'CG_TT Cuts at: 811 6694 811 Size: 5883 971 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 406 986 1152 2461 2726 3437 3497 3699 3742 Size: 580 166 1309 265 711 60 202 43 Cuts at: 3742 4679 5111 5464 6126 406 Size: 937 432 353 662 1134 Fragments arranged by size: 1309 1134 937 711 662 580 432 353 265 202 166 60 43 AflIII A'CryG_T Cuts at: 335 983 2489 4206 6006 6287 335 Size: 648 1506 1717 1800 281 902 Fragments arranged by size: 1800 1717 1506 902 648 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 320 785 2780 320 Size: 465 1995 4394 Fragments arranged by size: 4394 1995 465 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2976 3008 2976 Size: 32 6822 AlwNI CAG_nnn'CTG Cuts at: 384 2256 2751 2860 3838 4159 5597 384 Size: 1872 495 109 978 321 1438 1641 Fragments arranged by size: 1872 1641 1438 978 495 321 109 ApaI G_GGCC'C Cuts at: 2590 3127 3138 3343 2590 Size: 537 11 205 6101 Fragments arranged by size: 6101 537 205 11 ApaLI G'TGCA_C Cuts at: 1036 1789 5692 1036 Size: 753 3903 2198 Fragments arranged by size: 3903 2198 753 ApoI r'AATT_y Cuts at: 993 3044 3858 6207 6463 993 Size: 2051 814 2349 256 1384 Fragments arranged by size: 2349 2051 1384 814 256 AseI AT'TA_AT Cuts at: 20 20 Size: 6854 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6854 AvaI C'yCGr_G Cuts at: 132 151 157 169 1297 1539 2273 3301 3371 Size: 19 6 12 1128 242 734 1028 70 Cuts at: 3371 3517 3874 132 Size: 146 357 3112 Fragments arranged by size: 3112 1128 1028 734 357 242 146 70 19 12 6 BamHI G'GATC_C Cuts at: 2221 3879 2221 Size: 1658 5196 BanI G'GyrC_C Cuts at: 3001 3249 3694 3870 4284 4532 4567 3001 Size: 248 445 176 414 248 35 5288 Fragments arranged by size: 5288 445 414 248 248 176 35 BanII G_rGCy'C Cuts at: 152 158 170 176 200 1666 2366 2590 2848 Size: 6 12 6 24 1466 700 224 258 Cuts at: 2848 3127 3138 3299 3343 3868 4898 152 Size: 279 11 161 44 525 1030 2108 Fragments arranged by size: 2108 1466 1030 700 525 279 258 224 161 44 24 12 11 6 6 BbeI G_GCGC'C Cuts at: 3253 4288 4536 3253 Size: 1035 248 5571 Fragments arranged by size: 5571 1035 248 BbsI GAAGACnn'nnnn_ Cuts at: 527 632 1630 1958 2126 2725 527 Size: 105 998 328 168 599 4656 Fragments arranged by size: 4656 998 599 328 168 105 BciVI GTATCCnnnnn_n' Cuts at: 268 4752 5808 6854 268 Size: 4484 1056 1046 268 Fragments arranged by size: 4484 1056 1046 268 BclI T'GATC_A Cuts at: 707 4374 707 Size: 3667 3187 BfrBI ATG'CAT Cuts at: 2779 2779 Size: 6854 BglI GCCn_nnn'nGGC Cuts at: 3285 3285 Size: 6854 BglII A'GATC_T Cuts at: 4369 6406 4369 Size: 2037 4817 BlpI GC'TnA_GC Cuts at: 2654 2909 3023 3966 2654 Size: 255 114 943 5542 Fragments arranged by size: 5542 943 255 114 Bme1580I G_kGCm'C Cuts at: 1040 1793 2590 3004 3127 3138 3343 3372 4479 Size: 753 797 414 123 11 205 29 1107 Cuts at: 4479 4572 5696 1040 Size: 93 1124 2198 Fragments arranged by size: 2198 1124 1107 797 753 414 205 123 93 29 11 BmgBI CAC'GTC Cuts at: 249 336 416 1109 1941 2492 2664 249 Size: 87 80 693 832 551 172 4439 Fragments arranged by size: 4439 832 693 551 172 87 80 BmrI ACTGGGnnnn_n' Cuts at: 283 1802 2545 3122 3137 4066 4147 4482 6626 Size: 1519 743 577 15 929 81 335 2144 Cuts at: 6626 283 Size: 511 Fragments arranged by size: 2144 1519 929 743 577 511 335 81 15 BmtI G_CTAG'C Cuts at: 410 2336 410 Size: 1926 4928 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 246 1554 2182 2778 5221 246 Size: 1308 628 596 2443 1879 Fragments arranged by size: 2443 1879 1308 628 596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1104 2131 2602 2773 2860 2939 2971 3301 1104 Size: 1027 471 171 87 79 32 330 4657 Fragments arranged by size: 4657 1027 471 330 171 87 79 32 Bpu10I CC'TnA_GC Cuts at: 146 1306 146 Size: 1160 5694 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 721 1597 1816 1822 4030 4909 5623 5921 721 Size: 876 219 6 2208 879 714 298 1654 Fragments arranged by size: 2208 1654 879 876 714 298 219 6 BsaI GGTCTCn'nnnn_ Cuts at: 1300 2569 3366 6558 1300 Size: 1269 797 3192 1596 Fragments arranged by size: 3192 1596 1269 797 BsaAI yAC'GTr Cuts at: 984 1935 4207 984 Size: 951 2272 3631 Fragments arranged by size: 3631 2272 951 BsaBI GATnn'nnATC Cuts at: 6 256 4368 6405 6 Size: 250 4112 2037 455 Fragments arranged by size: 4112 2037 455 250 BsaHI Gr'CG_yC Cuts at: 1986 2944 3250 3527 4285 4533 5235 6731 1986 Size: 958 306 277 758 248 702 1496 2109 Fragments arranged by size: 2109 1496 958 758 702 306 277 248 BsaWI w'CCGG_w Cuts at: 192 4043 4564 5242 5653 5800 6074 192 Size: 3851 521 678 411 147 274 972 Fragments arranged by size: 3851 972 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 614 644 970 1000 1095 1125 1394 1424 3427 Size: 30 326 30 95 30 269 30 2003 Cuts at: 3427 3457 614 Size: 30 4011 Fragments arranged by size: 4011 2003 326 269 95 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 367 682 880 991 1972 2128 2470 3451 3793 Size: 315 198 111 981 156 342 981 342 Cuts at: 3793 367 Size: 3428 Fragments arranged by size: 3428 981 981 342 342 315 198 156 111 BseYI C'CCAG_C Cuts at: 752 958 2006 2365 2466 2744 3216 3336 4279 Size: 206 1048 359 101 278 472 120 943 Cuts at: 4279 5037 5702 6110 752 Size: 758 665 408 1496 Fragments arranged by size: 1496 1048 943 758 665 472 408 359 278 206 120 101 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1345 3394 3508 1345 Size: 2049 114 4691 Fragments arranged by size: 4691 2049 114 BsiEI CG_ry'CG Cuts at: 104 2726 4396 4442 5672 104 Size: 2622 1670 46 1230 1286 Fragments arranged by size: 2622 1670 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 983 1040 1666 1690 1793 1946 2057 3299 3326 Size: 57 626 24 103 153 111 1242 27 Cuts at: 3326 3868 4646 4836 5696 983 Size: 542 778 190 860 2141 Fragments arranged by size: 2141 1242 860 778 626 542 190 153 111 103 57 27 24 BsmI GAATG_Cn' Cuts at: 1559 3038 1559 Size: 1479 5375 BsmBI CGTCTCn'nnnn_ Cuts at: 1115 1477 3633 1115 Size: 362 2156 4336 Fragments arranged by size: 4336 2156 362 Bsp1286I G_dGCh'C Cuts at: 152 158 170 176 200 983 1040 1666 1690 Size: 6 12 6 24 783 57 626 24 Cuts at: 1690 1793 1946 2057 2366 2590 2848 3004 3127 Size: 103 153 111 309 224 258 156 123 Cuts at: 3127 3138 3299 3326 3343 3372 3868 4479 4572 Size: 11 161 27 17 29 496 611 93 Cuts at: 4572 4646 4836 4898 5696 152 Size: 74 190 62 798 1310 Fragments arranged by size: 1310 798 783 626 611 496 309 258 224 190 161 156 153 123 111 103 93 74 62 57 29 27 24 24 17 12 11 6 6 BspHI T'CATG_A Cuts at: 5286 5286 Size: 6854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1330 3904 4118 4420 4801 1330 Size: 2574 214 302 381 3383 Fragments arranged by size: 3383 2574 381 302 214 BsrBI CCG'CTC Cuts at: 50 672 1265 1673 3522 5146 5200 6684 6849 Size: 622 593 408 1849 1624 54 1484 165 Cuts at: 6849 50 Size: 55 Fragments arranged by size: 1849 1624 1484 622 593 408 165 55 54 BsrDI GCAATG_nn' Cuts at: 2410 2779 4766 2410 Size: 369 1987 4498 Fragments arranged by size: 4498 1987 369 BsrFI r'CCGG_y Cuts at: 758 999 3691 3766 4852 5033 758 Size: 241 2692 75 1086 181 2579 Fragments arranged by size: 2692 2579 1086 241 181 75 BsrGI T'GTAC_A Cuts at: 1412 1412 Size: 6854 BssSI C'ACGA_G Cuts at: 2971 5125 5833 2971 Size: 2154 708 3992 Fragments arranged by size: 3992 2154 708 BstAPI GCAn_nnn'nTGC Cuts at: 2664 3033 4231 2664 Size: 369 1198 5287 Fragments arranged by size: 5287 1198 369 BstBI TT'CG_AA Cuts at: 5215 5215 Size: 6854 BstXI CCAn_nnnn'nTGG Cuts at: 1128 1212 2019 1128 Size: 84 807 5963 Fragments arranged by size: 5963 807 84 BstYI r'GATC_y Cuts at: 1 2025 2221 3381 3548 3879 4346 4369 4704 Size: 2024 196 1160 167 331 467 23 335 Cuts at: 4704 4950 5256 5268 5354 6406 1 Size: 246 306 12 86 1052 449 Fragments arranged by size: 2024 1160 1052 467 449 335 331 306 246 196 167 86 23 12 Bsu36I CC'TnA_GG Cuts at: 3839 3839 Size: 6854 BtgI C'CryG_G Cuts at: 107 1311 1877 3119 3707 3845 107 Size: 1204 566 1242 588 138 3116 Fragments arranged by size: 3116 1242 1204 588 566 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 677 4804 4985 5148 677 Size: 4127 181 163 2383 Fragments arranged by size: 4127 2383 181 163 BtsI GCAGTG_nn' Cuts at: 2183 2304 4097 2183 Size: 121 1793 4940 Fragments arranged by size: 4940 1793 121 ClaI AT'CG_AT Cuts at: 284 1856 3425 284 Size: 1572 1569 3713 Fragments arranged by size: 3713 1572 1569 DraI TTT'AAA Cuts at: 3853 3853 Size: 6854 DraIII CAC_nnn'GTG Cuts at: 2515 3330 2515 Size: 815 6039 DrdI GACnn_nn'nnGTC Cuts at: 867 2767 4560 5904 867 Size: 1900 1793 1344 1817 Fragments arranged by size: 1900 1817 1793 1344 EaeI y'GGCC_r Cuts at: 790 1146 3459 4439 4613 5004 5031 6376 790 Size: 356 2313 980 174 391 27 1345 1268 Fragments arranged by size: 2313 1345 1268 980 391 356 174 27 EagI C'GGCC_G Cuts at: 4439 4439 Size: 6854 EarI CTCTTCn'nnn_ Cuts at: 437 506 1742 2736 3823 4877 5087 437 Size: 69 1236 994 1087 1054 210 2204 Fragments arranged by size: 2204 1236 1087 1054 994 210 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1008 1559 5062 5804 5950 6664 1008 Size: 551 3503 742 146 714 1198 Fragments arranged by size: 3503 1198 742 714 551 146 EcoICRI GAG'CTC Cuts at: 1664 3297 3866 1664 Size: 1633 569 4652 Fragments arranged by size: 4652 1633 569 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 406 986 1104 1152 2131 2461 2602 2726 2773 Size: 580 118 48 979 330 141 124 47 Cuts at: 2773 2860 2939 2971 3301 3437 3497 3699 3742 Size: 87 79 32 330 136 60 202 43 Cuts at: 3742 4679 5111 5464 6126 406 Size: 937 432 353 662 1134 Fragments arranged by size: 1134 979 937 662 580 432 353 330 330 202 141 136 124 118 87 79 60 48 47 43 32 EcoNI CCTnn'n_nnAGG Cuts at: 1273 1760 3239 3760 1273 Size: 487 1479 521 4367 Fragments arranged by size: 4367 1479 521 487 EcoO109I rG'GnC_Cy Cuts at: 381 1188 1301 1883 2145 2253 2586 2587 3124 Size: 807 113 582 262 108 333 1 537 Cuts at: 3124 3134 3135 3835 3993 381 Size: 10 1 700 158 3242 Fragments arranged by size: 3242 807 700 582 537 333 262 158 113 108 10 1 1 EcoRI G'AATT_C Cuts at: 993 3858 993 Size: 2865 3989 EcoRV GAT'ATC Cuts at: 1851 1851 Size: 6854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 466 4873 466 Size: 4407 2447 FspI TGC'GCA Cuts at: 4635 4635 Size: 6854 HaeII r_GCGC'y Cuts at: 467 3253 4288 4536 5766 6537 467 Size: 2786 1035 248 1230 771 784 Fragments arranged by size: 2786 1230 1035 784 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 808 840 1000 1032 1081 1113 1810 1838 1842 Size: 32 160 32 49 32 697 28 4 Cuts at: 1842 1870 4696 4726 4728 4758 808 Size: 28 2826 30 2 30 2904 Fragments arranged by size: 2904 2826 697 160 49 32 32 32 30 30 28 28 4 2 HincII GTy'rAC Cuts at: 473 2244 2772 3893 473 Size: 1771 528 1121 3434 Fragments arranged by size: 3434 1771 1121 528 KasI G'GCGC_C Cuts at: 3249 4284 4532 3249 Size: 1035 248 5571 Fragments arranged by size: 5571 1035 248 KpnI G_GTAC'C Cuts at: 3874 3874 Size: 6854 MfeI C'AATT_G Cuts at: 713 713 Size: 6854 MluI A'CGCG_T Cuts at: 6287 6287 Size: 6854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 356 1709 1969 5613 5797 356 Size: 1353 260 3644 184 1413 Fragments arranged by size: 3644 1413 1353 260 184 MscI TGG'CCA Cuts at: 792 4615 792 Size: 3823 3031 MslI CAynn'nnrTG Cuts at: 1933 4843 4970 1933 Size: 2910 127 3817 Fragments arranged by size: 3817 2910 127 MspA1I CmG'CkG Cuts at: 127 426 3464 3709 3965 4279 4639 5423 5668 Size: 299 3038 245 256 314 360 784 245 Cuts at: 5668 127 Size: 1313 Fragments arranged by size: 3038 1313 784 360 314 299 256 245 245 NaeI GCC'GGC Cuts at: 3693 3768 5035 3693 Size: 75 1267 5512 Fragments arranged by size: 5512 1267 75 NarI GG'CG_CC Cuts at: 3250 4285 4533 3250 Size: 1035 248 5571 Fragments arranged by size: 5571 1035 248 NcoI C'CATG_G Cuts at: 107 1877 3119 3845 107 Size: 1770 1242 726 3116 Fragments arranged by size: 3116 1770 1242 726 NgoMIV G'CCGG_C Cuts at: 3691 3766 5033 3691 Size: 75 1267 5512 Fragments arranged by size: 5512 1267 75 NheI G'CTAG_C Cuts at: 406 2332 406 Size: 1926 4928 NsiI A_TGCA'T Cuts at: 2781 2781 Size: 6854 NspI r_CATG'y Cuts at: 2061 3907 4407 6010 2061 Size: 1846 500 1603 2905 Fragments arranged by size: 2905 1846 1603 500 PasI CC'CwG_GG Cuts at: 2997 2997 Size: 6854 PciI A'CATG_T Cuts at: 6006 6006 Size: 6854 PflMI CCAn_nnn'nTGG Cuts at: 384 2598 2625 2751 2997 3598 384 Size: 2214 27 126 246 601 3640 Fragments arranged by size: 3640 2214 601 246 126 27 PfoI T'CCnGG_A Cuts at: 260 1004 2030 2916 3317 260 Size: 744 1026 886 401 3797 Fragments arranged by size: 3797 1026 886 744 401 PmeI GTTT'AAAC Cuts at: 3853 3853 Size: 6854 PmlI CAC'GTG Cuts at: 984 984 Size: 6854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5300 5332 5300 Size: 32 6822 PpuMI rG'GwC_Cy Cuts at: 381 1301 1883 2145 381 Size: 920 582 262 5090 Fragments arranged by size: 5090 920 582 262 PspOMI G'GGCC_C Cuts at: 2586 3123 3134 3339 2586 Size: 537 11 205 6101 Fragments arranged by size: 6101 537 205 11 PstI C_TGCA'G Cuts at: 1327 3810 3901 4586 1327 Size: 2483 91 685 3595 Fragments arranged by size: 3595 2483 685 91 PvuI CG_AT'CG Cuts at: 104 104 Size: 6854 PvuII CAG'CTG Cuts at: 127 426 4279 4639 127 Size: 299 3853 360 2342 Fragments arranged by size: 3853 2342 360 299 RsrII CG'GwC_CG Cuts at: 1016 5049 1016 Size: 4033 2821 SacI G_AGCT'C Cuts at: 1666 3299 3868 1666 Size: 1633 569 4652 Fragments arranged by size: 4652 1633 569 SacII CC_GC'GG Cuts at: 3710 3710 Size: 6854 SalI G'TCGA_C Cuts at: 2770 3891 2770 Size: 1121 5733 SanDI GG'GwC_CC Cuts at: 1301 2145 1301 Size: 844 6010 SapI GCTCTTCn'nnn_ Cuts at: 1742 4877 5087 1742 Size: 3135 210 3509 Fragments arranged by size: 3509 3135 210 SbfI CC_TGCA'GG Cuts at: 3901 3901 Size: 6854 ScaI AGT'ACT Cuts at: 6371 6371 Size: 6854 SexAI A'CCwGG_T Cuts at: 2511 2511 Size: 6854 SfcI C'TryA_G Cuts at: 33 459 530 1323 1628 3806 3897 4582 5550 Size: 426 71 793 305 2178 91 685 968 Cuts at: 5550 5741 33 Size: 191 1146 Fragments arranged by size: 2178 1146 968 793 685 426 305 191 91 71 SfoI GGC'GCC Cuts at: 3251 4286 4534 3251 Size: 1035 248 5571 Fragments arranged by size: 5571 1035 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6854 SmaI CCC'GGG Cuts at: 134 3373 3876 134 Size: 3239 503 3112 Fragments arranged by size: 3239 3112 503 SmlI C'TyrA_G Cuts at: 736 1612 1831 1837 4009 4924 5638 5900 736 Size: 876 219 6 2172 915 714 262 1690 Fragments arranged by size: 2172 1690 915 876 714 262 219 6 SpeI A'CTAG_T Cuts at: 67 67 Size: 6854 SphI G_CATG'C Cuts at: 3907 4407 3907 Size: 500 6354 StuI AGG'CCT Cuts at: 3234 3234 Size: 6854 StyI C'CwwG_G Cuts at: 107 1877 3119 3845 3988 4342 107 Size: 1770 1242 726 143 354 2619 Fragments arranged by size: 2619 1770 1242 726 354 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 259 1890 2458 3626 3664 4437 4570 5029 5238 Size: 1631 568 1168 38 773 133 459 209 Cuts at: 5238 259 Size: 1875 Fragments arranged by size: 1875 1631 1168 773 568 459 209 133 38 TatI w'GTAC_w Cuts at: 1412 6369 1412 Size: 4957 1897 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1095 1127 1394 1426 1794 1826 6371 6403 1095 Size: 32 267 32 368 32 4545 32 1546 Fragments arranged by size: 4545 1546 368 267 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4651 4651 Size: 6854 XbaI T'CTAG_A Cuts at: 3384 3885 3384 Size: 501 6353 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 4349 2257 Size: 2092 4762 XmaI C'CCGG_G Cuts at: 132 3371 3874 132 Size: 3239 503 3112 Fragments arranged by size: 3239 3112 503 XmnI GAAnn'nnTTC Cuts at: 1637 1637 Size: 6854 ZraI GAC'GTC Cuts at: 3528 3528 Size: 6854 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflIII AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AflII AgeI AhdI AleI AscI AvrII BaeI BbvCI BcgI BsiWI BspEI BssHII BstEII BstZ17I CspCI FseI FspAI HindIII HpaI NdeI NotI NruI PacI PshAI PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI