Restriction map for pF1KA0790
 (Circular) (Six-base) MAPSORT of: pF1KA0790.seq  Check: 40  from: 1  to: 6854



pF1KA0790  6854 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:20  ..

AatII G_ACGT'C

Cuts at:   3530    3530 
   Size:       6854
 
AccI GT'mk_AC

Cuts at:   2771    3892    2771 
   Size:       1121    5733
 
Acc65I G'GTAC_C

Cuts at:   3870    3870 
   Size:       6854
 
AclI AA'CG_TT

Cuts at:    811    6694     811 
   Size:       5883     971
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    406     986    1152    2461    2726    3437    3497    3699    3742 
   Size:        580     166    1309     265     711      60     202      43

Cuts at:   3742    4679    5111    5464    6126     406 
   Size:        937     432     353     662    1134

  Fragments arranged by size: 

                1309    1134     937     711     662     580     432     353
                 265     202     166      60      43
 
AflIII A'CryG_T

Cuts at:    335     983    2489    4206    6006    6287     335 
   Size:        648    1506    1717    1800     281     902

  Fragments arranged by size: 

                1800    1717    1506     902     648     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    320     785    2780     320 
   Size:        465    1995    4394

  Fragments arranged by size: 

                4394    1995     465
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2976    3008    2976 
   Size:         32    6822
 
AlwNI CAG_nnn'CTG

Cuts at:    384    2256    2751    2860    3838    4159    5597     384 
   Size:       1872     495     109     978     321    1438    1641

  Fragments arranged by size: 

                1872    1641    1438     978     495     321     109
 
ApaI G_GGCC'C

Cuts at:   2590    3127    3138    3343    2590 
   Size:        537      11     205    6101

  Fragments arranged by size: 

                6101     537     205      11
 
ApaLI G'TGCA_C

Cuts at:   1036    1789    5692    1036 
   Size:        753    3903    2198

  Fragments arranged by size: 

                3903    2198     753
 
ApoI r'AATT_y

Cuts at:    993    3044    3858    6207    6463     993 
   Size:       2051     814    2349     256    1384

  Fragments arranged by size: 

                2349    2051    1384     814     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       6854
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6854
 
AvaI C'yCGr_G

Cuts at:    132     151     157     169    1297    1539    2273    3301    3371 
   Size:         19       6      12    1128     242     734    1028      70

Cuts at:   3371    3517    3874     132 
   Size:        146     357    3112

  Fragments arranged by size: 

                3112    1128    1028     734     357     242     146      70
                  19      12       6
 
BamHI G'GATC_C

Cuts at:   2221    3879    2221 
   Size:       1658    5196
 
BanI G'GyrC_C

Cuts at:   3001    3249    3694    3870    4284    4532    4567    3001 
   Size:        248     445     176     414     248      35    5288

  Fragments arranged by size: 

                5288     445     414     248     248     176      35
 
BanII G_rGCy'C

Cuts at:    152     158     170     176     200    1666    2366    2590    2848 
   Size:          6      12       6      24    1466     700     224     258

Cuts at:   2848    3127    3138    3299    3343    3868    4898     152 
   Size:        279      11     161      44     525    1030    2108

  Fragments arranged by size: 

                2108    1466    1030     700     525     279     258     224
                 161      44      24      12      11       6       6
 
BbeI G_GCGC'C

Cuts at:   3253    4288    4536    3253 
   Size:       1035     248    5571

  Fragments arranged by size: 

                5571    1035     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    527     632    1630    1958    2126    2725     527 
   Size:        105     998     328     168     599    4656

  Fragments arranged by size: 

                4656     998     599     328     168     105
 
BciVI GTATCCnnnnn_n'

Cuts at:    268    4752    5808    6854     268 
   Size:       4484    1056    1046     268

  Fragments arranged by size: 

                4484    1056    1046     268
 
BclI T'GATC_A

Cuts at:    707    4374     707 
   Size:       3667    3187
 
BfrBI ATG'CAT

Cuts at:   2779    2779 
   Size:       6854
 
BglI GCCn_nnn'nGGC

Cuts at:   3285    3285 
   Size:       6854
 
BglII A'GATC_T

Cuts at:   4369    6406    4369 
   Size:       2037    4817
 
BlpI GC'TnA_GC

Cuts at:   2654    2909    3023    3966    2654 
   Size:        255     114     943    5542

  Fragments arranged by size: 

                5542     943     255     114
 
Bme1580I G_kGCm'C

Cuts at:   1040    1793    2590    3004    3127    3138    3343    3372    4479 
   Size:        753     797     414     123      11     205      29    1107

Cuts at:   4479    4572    5696    1040 
   Size:         93    1124    2198

  Fragments arranged by size: 

                2198    1124    1107     797     753     414     205     123
                  93      29      11
 
BmgBI CAC'GTC

Cuts at:    249     336     416    1109    1941    2492    2664     249 
   Size:         87      80     693     832     551     172    4439

  Fragments arranged by size: 

                4439     832     693     551     172      87      80
 
BmrI ACTGGGnnnn_n'

Cuts at:    283    1802    2545    3122    3137    4066    4147    4482    6626 
   Size:       1519     743     577      15     929      81     335    2144

Cuts at:   6626     283 
   Size:        511

  Fragments arranged by size: 

                2144    1519     929     743     577     511     335      81
                  15
 
BmtI G_CTAG'C

Cuts at:    410    2336     410 
   Size:       1926    4928
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    246    1554    2182    2778    5221     246 
   Size:       1308     628     596    2443    1879

  Fragments arranged by size: 

                2443    1879    1308     628     596
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1104    2131    2602    2773    2860    2939    2971    3301    1104 
   Size:       1027     471     171      87      79      32     330    4657

  Fragments arranged by size: 

                4657    1027     471     330     171      87      79      32
            
 
Bpu10I CC'TnA_GC

Cuts at:    146    1306     146 
   Size:       1160    5694
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    721    1597    1816    1822    4030    4909    5623    5921     721 
   Size:        876     219       6    2208     879     714     298    1654

  Fragments arranged by size: 

                2208    1654     879     876     714     298     219       6
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   1300    2569    3366    6558    1300 
   Size:       1269     797    3192    1596

  Fragments arranged by size: 

                3192    1596    1269     797
 
BsaAI yAC'GTr

Cuts at:    984    1935    4207     984 
   Size:        951    2272    3631

  Fragments arranged by size: 

                3631    2272     951
 
BsaBI GATnn'nnATC

Cuts at:      6     256    4368    6405       6 
   Size:        250    4112    2037     455

  Fragments arranged by size: 

                4112    2037     455     250
 
BsaHI Gr'CG_yC

Cuts at:   1986    2944    3250    3527    4285    4533    5235    6731    1986 
   Size:        958     306     277     758     248     702    1496    2109

  Fragments arranged by size: 

                2109    1496     958     758     702     306     277     248
            
 
BsaWI w'CCGG_w

Cuts at:    192    4043    4564    5242    5653    5800    6074     192 
   Size:       3851     521     678     411     147     274     972

  Fragments arranged by size: 

                3851     972     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    614     644     970    1000    1095    1125    1394    1424    3427 
   Size:         30     326      30      95      30     269      30    2003

Cuts at:   3427    3457     614 
   Size:         30    4011

  Fragments arranged by size: 

                4011    2003     326     269      95      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    367     682     880     991    1972    2128    2470    3451    3793 
   Size:        315     198     111     981     156     342     981     342

Cuts at:   3793     367 
   Size:       3428

  Fragments arranged by size: 

                3428     981     981     342     342     315     198     156
                 111
 
BseYI C'CCAG_C

Cuts at:    752     958    2006    2365    2466    2744    3216    3336    4279 
   Size:        206    1048     359     101     278     472     120     943

Cuts at:   4279    5037    5702    6110     752 
   Size:        758     665     408    1496

  Fragments arranged by size: 

                1496    1048     943     758     665     472     408     359
                 278     206     120     101
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1345    3394    3508    1345 
   Size:       2049     114    4691

  Fragments arranged by size: 

                4691    2049     114
 
BsiEI CG_ry'CG

Cuts at:    104    2726    4396    4442    5672     104 
   Size:       2622    1670      46    1230    1286

  Fragments arranged by size: 

                2622    1670    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:    983    1040    1666    1690    1793    1946    2057    3299    3326 
   Size:         57     626      24     103     153     111    1242      27

Cuts at:   3326    3868    4646    4836    5696     983 
   Size:        542     778     190     860    2141

  Fragments arranged by size: 

                2141    1242     860     778     626     542     190     153
                 111     103      57      27      24
 
BsmI GAATG_Cn'

Cuts at:   1559    3038    1559 
   Size:       1479    5375
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1115    1477    3633    1115 
   Size:        362    2156    4336

  Fragments arranged by size: 

                4336    2156     362
 
Bsp1286I G_dGCh'C

Cuts at:    152     158     170     176     200     983    1040    1666    1690 
   Size:          6      12       6      24     783      57     626      24

Cuts at:   1690    1793    1946    2057    2366    2590    2848    3004    3127 
   Size:        103     153     111     309     224     258     156     123

Cuts at:   3127    3138    3299    3326    3343    3372    3868    4479    4572 
   Size:         11     161      27      17      29     496     611      93

Cuts at:   4572    4646    4836    4898    5696     152 
   Size:         74     190      62     798    1310

  Fragments arranged by size: 

                1310     798     783     626     611     496     309     258
                 224     190     161     156     153     123     111     103
                  93      74      62      57      29      27      24      24
                  17      12      11       6       6
 
BspHI T'CATG_A

Cuts at:   5286    5286 
   Size:       6854
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1330    3904    4118    4420    4801    1330 
   Size:       2574     214     302     381    3383

  Fragments arranged by size: 

                3383    2574     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     672    1265    1673    3522    5146    5200    6684    6849 
   Size:        622     593     408    1849    1624      54    1484     165

Cuts at:   6849      50 
   Size:         55

  Fragments arranged by size: 

                1849    1624    1484     622     593     408     165      55
                  54
 
BsrDI GCAATG_nn'

Cuts at:   2410    2779    4766    2410 
   Size:        369    1987    4498

  Fragments arranged by size: 

                4498    1987     369
 
BsrFI r'CCGG_y

Cuts at:    758     999    3691    3766    4852    5033     758 
   Size:        241    2692      75    1086     181    2579

  Fragments arranged by size: 

                2692    2579    1086     241     181      75
 
BsrGI T'GTAC_A

Cuts at:   1412    1412 
   Size:       6854
 
BssSI C'ACGA_G

Cuts at:   2971    5125    5833    2971 
   Size:       2154     708    3992

  Fragments arranged by size: 

                3992    2154     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2664    3033    4231    2664 
   Size:        369    1198    5287

  Fragments arranged by size: 

                5287    1198     369
 
BstBI TT'CG_AA

Cuts at:   5215    5215 
   Size:       6854
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1128    1212    2019    1128 
   Size:         84     807    5963

  Fragments arranged by size: 

                5963     807      84
 
BstYI r'GATC_y

Cuts at:      1    2025    2221    3381    3548    3879    4346    4369    4704 
   Size:       2024     196    1160     167     331     467      23     335

Cuts at:   4704    4950    5256    5268    5354    6406       1 
   Size:        246     306      12      86    1052     449

  Fragments arranged by size: 

                2024    1160    1052     467     449     335     331     306
                 246     196     167      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   3839    3839 
   Size:       6854
 
BtgI C'CryG_G

Cuts at:    107    1311    1877    3119    3707    3845     107 
   Size:       1204     566    1242     588     138    3116

  Fragments arranged by size: 

                3116    1242    1204     588     566     138
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    677    4804    4985    5148     677 
   Size:       4127     181     163    2383

  Fragments arranged by size: 

                4127    2383     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2183    2304    4097    2183 
   Size:        121    1793    4940

  Fragments arranged by size: 

                4940    1793     121
 
ClaI AT'CG_AT

Cuts at:    284    1856    3425     284 
   Size:       1572    1569    3713

  Fragments arranged by size: 

                3713    1572    1569
 
DraI TTT'AAA

Cuts at:   3853    3853 
   Size:       6854
 
DraIII CAC_nnn'GTG

Cuts at:   2515    3330    2515 
   Size:        815    6039
 
DrdI GACnn_nn'nnGTC

Cuts at:    867    2767    4560    5904     867 
   Size:       1900    1793    1344    1817

  Fragments arranged by size: 

                1900    1817    1793    1344
 
EaeI y'GGCC_r

Cuts at:    790    1146    3459    4439    4613    5004    5031    6376     790 
   Size:        356    2313     980     174     391      27    1345    1268

  Fragments arranged by size: 

                2313    1345    1268     980     391     356     174      27
            
 
EagI C'GGCC_G

Cuts at:   4439    4439 
   Size:       6854
 
EarI CTCTTCn'nnn_

Cuts at:    437     506    1742    2736    3823    4877    5087     437 
   Size:         69    1236     994    1087    1054     210    2204

  Fragments arranged by size: 

                2204    1236    1087    1054     994     210      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1008    1559    5062    5804    5950    6664    1008 
   Size:        551    3503     742     146     714    1198

  Fragments arranged by size: 

                3503    1198     742     714     551     146
 
EcoICRI GAG'CTC

Cuts at:   1664    3297    3866    1664 
   Size:       1633     569    4652

  Fragments arranged by size: 

                4652    1633     569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    406     986    1104    1152    2131    2461    2602    2726    2773 
   Size:        580     118      48     979     330     141     124      47

Cuts at:   2773    2860    2939    2971    3301    3437    3497    3699    3742 
   Size:         87      79      32     330     136      60     202      43

Cuts at:   3742    4679    5111    5464    6126     406 
   Size:        937     432     353     662    1134

  Fragments arranged by size: 

                1134     979     937     662     580     432     353     330
                 330     202     141     136     124     118      87      79
                  60      48      47      43      32
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1273    1760    3239    3760    1273 
   Size:        487    1479     521    4367

  Fragments arranged by size: 

                4367    1479     521     487
 
EcoO109I rG'GnC_Cy

Cuts at:    381    1188    1301    1883    2145    2253    2586    2587    3124 
   Size:        807     113     582     262     108     333       1     537

Cuts at:   3124    3134    3135    3835    3993     381 
   Size:         10       1     700     158    3242

  Fragments arranged by size: 

                3242     807     700     582     537     333     262     158
                 113     108      10       1       1
 
EcoRI G'AATT_C

Cuts at:    993    3858     993 
   Size:       2865    3989
 
EcoRV GAT'ATC

Cuts at:   1851    1851 
   Size:       6854
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    466    4873     466 
   Size:       4407    2447
 
FspI TGC'GCA

Cuts at:   4635    4635 
   Size:       6854
 
HaeII r_GCGC'y

Cuts at:    467    3253    4288    4536    5766    6537     467 
   Size:       2786    1035     248    1230     771     784

  Fragments arranged by size: 

                2786    1230    1035     784     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    808     840    1000    1032    1081    1113    1810    1838    1842 
   Size:         32     160      32      49      32     697      28       4

Cuts at:   1842    1870    4696    4726    4728    4758     808 
   Size:         28    2826      30       2      30    2904

  Fragments arranged by size: 

                2904    2826     697     160      49      32      32      32
                  30      30      28      28       4       2
 
HincII GTy'rAC

Cuts at:    473    2244    2772    3893     473 
   Size:       1771     528    1121    3434

  Fragments arranged by size: 

                3434    1771    1121     528
 
KasI G'GCGC_C

Cuts at:   3249    4284    4532    3249 
   Size:       1035     248    5571

  Fragments arranged by size: 

                5571    1035     248
 
KpnI G_GTAC'C

Cuts at:   3874    3874 
   Size:       6854
 
MfeI C'AATT_G

Cuts at:    713     713 
   Size:       6854
 
MluI A'CGCG_T

Cuts at:   6287    6287 
   Size:       6854
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    356    1709    1969    5613    5797     356 
   Size:       1353     260    3644     184    1413

  Fragments arranged by size: 

                3644    1413    1353     260     184
 
MscI TGG'CCA

Cuts at:    792    4615     792 
   Size:       3823    3031
 
MslI CAynn'nnrTG

Cuts at:   1933    4843    4970    1933 
   Size:       2910     127    3817

  Fragments arranged by size: 

                3817    2910     127
 
MspA1I CmG'CkG

Cuts at:    127     426    3464    3709    3965    4279    4639    5423    5668 
   Size:        299    3038     245     256     314     360     784     245

Cuts at:   5668     127 
   Size:       1313

  Fragments arranged by size: 

                3038    1313     784     360     314     299     256     245
                 245
 
NaeI GCC'GGC

Cuts at:   3693    3768    5035    3693 
   Size:         75    1267    5512

  Fragments arranged by size: 

                5512    1267      75
 
NarI GG'CG_CC

Cuts at:   3250    4285    4533    3250 
   Size:       1035     248    5571

  Fragments arranged by size: 

                5571    1035     248
 
NcoI C'CATG_G

Cuts at:    107    1877    3119    3845     107 
   Size:       1770    1242     726    3116

  Fragments arranged by size: 

                3116    1770    1242     726
 
NgoMIV G'CCGG_C

Cuts at:   3691    3766    5033    3691 
   Size:         75    1267    5512

  Fragments arranged by size: 

                5512    1267      75
 
NheI G'CTAG_C

Cuts at:    406    2332     406 
   Size:       1926    4928
 
NsiI A_TGCA'T

Cuts at:   2781    2781 
   Size:       6854
 
NspI r_CATG'y

Cuts at:   2061    3907    4407    6010    2061 
   Size:       1846     500    1603    2905

  Fragments arranged by size: 

                2905    1846    1603     500
 
PasI CC'CwG_GG

Cuts at:   2997    2997 
   Size:       6854
 
PciI A'CATG_T

Cuts at:   6006    6006 
   Size:       6854
 
PflMI CCAn_nnn'nTGG

Cuts at:    384    2598    2625    2751    2997    3598     384 
   Size:       2214      27     126     246     601    3640

  Fragments arranged by size: 

                3640    2214     601     246     126      27
 
PfoI T'CCnGG_A

Cuts at:    260    1004    2030    2916    3317     260 
   Size:        744    1026     886     401    3797

  Fragments arranged by size: 

                3797    1026     886     744     401
 
PmeI GTTT'AAAC

Cuts at:   3853    3853 
   Size:       6854
 
PmlI CAC'GTG

Cuts at:    984     984 
   Size:       6854
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5300    5332    5300 
   Size:         32    6822
 
PpuMI rG'GwC_Cy

Cuts at:    381    1301    1883    2145     381 
   Size:        920     582     262    5090

  Fragments arranged by size: 

                5090     920     582     262
 
PspOMI G'GGCC_C

Cuts at:   2586    3123    3134    3339    2586 
   Size:        537      11     205    6101

  Fragments arranged by size: 

                6101     537     205      11
 
PstI C_TGCA'G

Cuts at:   1327    3810    3901    4586    1327 
   Size:       2483      91     685    3595

  Fragments arranged by size: 

                3595    2483     685      91
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       6854
 
PvuII CAG'CTG

Cuts at:    127     426    4279    4639     127 
   Size:        299    3853     360    2342

  Fragments arranged by size: 

                3853    2342     360     299
 
RsrII CG'GwC_CG

Cuts at:   1016    5049    1016 
   Size:       4033    2821
 
SacI G_AGCT'C

Cuts at:   1666    3299    3868    1666 
   Size:       1633     569    4652

  Fragments arranged by size: 

                4652    1633     569
 
SacII CC_GC'GG

Cuts at:   3710    3710 
   Size:       6854
 
SalI G'TCGA_C

Cuts at:   2770    3891    2770 
   Size:       1121    5733
 
SanDI GG'GwC_CC

Cuts at:   1301    2145    1301 
   Size:        844    6010
 
SapI GCTCTTCn'nnn_

Cuts at:   1742    4877    5087    1742 
   Size:       3135     210    3509

  Fragments arranged by size: 

                3509    3135     210
 
SbfI CC_TGCA'GG

Cuts at:   3901    3901 
   Size:       6854
 
ScaI AGT'ACT

Cuts at:   6371    6371 
   Size:       6854
 
SexAI A'CCwGG_T

Cuts at:   2511    2511 
   Size:       6854
 
SfcI C'TryA_G

Cuts at:     33     459     530    1323    1628    3806    3897    4582    5550 
   Size:        426      71     793     305    2178      91     685     968

Cuts at:   5550    5741      33 
   Size:        191    1146

  Fragments arranged by size: 

                2178    1146     968     793     685     426     305     191
                  91      71
 
SfoI GGC'GCC

Cuts at:   3251    4286    4534    3251 
   Size:       1035     248    5571

  Fragments arranged by size: 

                5571    1035     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       6854
 
SmaI CCC'GGG

Cuts at:    134    3373    3876     134 
   Size:       3239     503    3112

  Fragments arranged by size: 

                3239    3112     503
 
SmlI C'TyrA_G

Cuts at:    736    1612    1831    1837    4009    4924    5638    5900     736 
   Size:        876     219       6    2172     915     714     262    1690

  Fragments arranged by size: 

                2172    1690     915     876     714     262     219       6
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       6854
 
SphI G_CATG'C

Cuts at:   3907    4407    3907 
   Size:        500    6354
 
StuI AGG'CCT

Cuts at:   3234    3234 
   Size:       6854
 
StyI C'CwwG_G

Cuts at:    107    1877    3119    3845    3988    4342     107 
   Size:       1770    1242     726     143     354    2619

  Fragments arranged by size: 

                2619    1770    1242     726     354     143
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    259    1890    2458    3626    3664    4437    4570    5029    5238 
   Size:       1631     568    1168      38     773     133     459     209

Cuts at:   5238     259 
   Size:       1875

  Fragments arranged by size: 

                1875    1631    1168     773     568     459     209     133
                  38
 
TatI w'GTAC_w

Cuts at:   1412    6369    1412 
   Size:       4957    1897
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1095    1127    1394    1426    1794    1826    6371    6403    1095 
   Size:         32     267      32     368      32    4545      32    1546

  Fragments arranged by size: 

                4545    1546     368     267      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   4651    4651 
   Size:       6854
 
XbaI T'CTAG_A

Cuts at:   3384    3885    3384 
   Size:        501    6353
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2257    4349    2257 
   Size:       2092    4762
 
XmaI C'CCGG_G

Cuts at:    132    3371    3874     132 
   Size:       3239     503    3112

  Fragments arranged by size: 

                3239    3112     503
 
XmnI GAAnn'nnTTC

Cuts at:   1637    1637 
   Size:       6854
 
ZraI GAC'GTC

Cuts at:   3528    3528 
   Size:       6854
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AgeI     AhdI     AleI     AscI    AvrII
     BaeI    BbvCI     BcgI    BsiWI    BspEI   BssHII   BstEII  BstZ17I
    CspCI     FseI    FspAI  HindIII     HpaI     NdeI     NotI     NruI
     PacI    PshAI     PsiI    PspXI     PsrI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI
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