(Circular) (Six-base) MAPSORT of: pF1KA0800.seq Check: 4205 from: 1 to: 7634 pF1KA0800 7634 bp With 182 enzymes: SgfI * June 20, 2008 10:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3190 3406 3451 3190 Size: 216 45 7373 Fragments arranged by size: 7373 216 45 AccI GT'mk_AC Cuts at: 4672 4672 Size: 7634 Acc65I G'GTAC_C Cuts at: 201 4650 201 Size: 4449 3185 AclI AA'CG_TT Cuts at: 7474 7474 Size: 7634 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 266 1213 1454 1720 1892 2137 3478 5459 5891 Size: 947 241 266 172 245 1341 1981 432 Cuts at: 5891 6244 6906 266 Size: 353 662 994 Fragments arranged by size: 1981 1341 994 947 662 432 353 266 245 241 172 AflIII A'CryG_T Cuts at: 126 2042 4986 6786 7067 126 Size: 1916 2944 1800 281 693 Fragments arranged by size: 2944 1916 1800 693 281 AgeI A'CCGG_T Cuts at: 1339 1339 Size: 7634 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1693 2332 1693 Size: 639 6995 AlwNI CAG_nnn'CTG Cuts at: 2456 2472 2779 4287 4404 4939 6377 2456 Size: 16 307 1508 117 535 1438 3713 Fragments arranged by size: 3713 1508 1438 535 307 117 16 ApaI G_GGCC'C Cuts at: 516 2376 516 Size: 1860 5774 ApaLI G'TGCA_C Cuts at: 1613 6472 1613 Size: 4859 2775 ApoI r'AATT_y Cuts at: 609 1264 1276 1596 1825 2135 2209 4638 6987 Size: 655 12 320 229 310 74 2429 2349 Cuts at: 6987 7243 609 Size: 256 1000 Fragments arranged by size: 2429 2349 1000 655 320 310 256 229 74 12 AseI AT'TA_AT Cuts at: 20 3932 20 Size: 3912 3722 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 7634 AvaI C'yCGr_G Cuts at: 515 4654 515 Size: 4139 3495 BamHI G'GATC_C Cuts at: 264 4659 264 Size: 4395 3239 BanI G'GyrC_C Cuts at: 201 1045 2004 2866 3052 3326 3439 4650 5064 Size: 844 959 862 186 274 113 1211 414 Cuts at: 5064 5312 5347 201 Size: 248 35 2488 Fragments arranged by size: 2488 1211 959 862 844 414 274 248 186 113 35 BanII G_rGCy'C Cuts at: 149 404 516 1535 2376 4648 5678 149 Size: 255 112 1019 841 2272 1030 2105 Fragments arranged by size: 2272 2105 1030 1019 841 255 112 BbeI G_GCGC'C Cuts at: 5068 5316 5068 Size: 248 7386 BbsI GAAGACnn'nnnn_ Cuts at: 645 1546 1945 4535 645 Size: 901 399 2590 3744 Fragments arranged by size: 3744 2590 901 399 BbvCI CC'TCA_GC Cuts at: 2105 2900 2105 Size: 795 6839 BciVI GTATCCnnnnn_n' Cuts at: 5532 6588 7634 5532 Size: 1056 1046 5532 Fragments arranged by size: 5532 1056 1046 BclI T'GATC_A Cuts at: 1574 3926 5154 1574 Size: 2352 1228 4054 Fragments arranged by size: 4054 2352 1228 BfrBI ATG'CAT Cuts at: 379 1404 379 Size: 1025 6609 BglI GCCn_nnn'nGGC Cuts at: 520 520 Size: 7634 BglII A'GATC_T Cuts at: 821 3759 5149 7186 821 Size: 2938 1390 2037 1269 Fragments arranged by size: 2938 2037 1390 1269 BlpI GC'TnA_GC Cuts at: 143 4746 143 Size: 4603 3031 Bme1580I G_kGCm'C Cuts at: 516 1617 2376 5259 5352 6476 516 Size: 1101 759 2883 93 1124 1674 Fragments arranged by size: 2883 1674 1124 1101 759 93 BmgBI CAC'GTC Cuts at: 2043 2043 Size: 7634 BmrI ACTGGGnnnn_n' Cuts at: 689 1664 2619 2767 4846 4927 5262 7406 689 Size: 975 955 148 2079 81 335 2144 917 Fragments arranged by size: 2144 2079 975 955 917 335 148 81 BmtI G_CTAG'C Cuts at: 1646 2929 4492 1646 Size: 1283 1563 4788 Fragments arranged by size: 4788 1563 1283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6001 6001 Size: 7634 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 181 830 2005 3940 4426 181 Size: 649 1175 1935 486 3389 Fragments arranged by size: 3389 1935 1175 649 486 Bpu10I CC'TnA_GC Cuts at: 2105 2900 3221 2105 Size: 795 321 6518 Fragments arranged by size: 6518 795 321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4810 5689 6403 6701 4810 Size: 879 714 298 5743 Fragments arranged by size: 5743 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 785 7338 785 Size: 6553 1081 BsaAI yAC'GTr Cuts at: 4987 4987 Size: 7634 BsaBI GATnn'nnATC Cuts at: 6 287 983 2161 2347 5148 7185 6 Size: 281 696 1178 186 2801 2037 455 Fragments arranged by size: 2801 2037 1178 696 455 281 186 BsaHI Gr'CG_yC Cuts at: 3830 5065 5313 6015 7511 3830 Size: 1235 248 702 1496 3953 Fragments arranged by size: 3953 1496 1235 702 248 BsaWI w'CCGG_w Cuts at: 1339 4823 5344 6022 6433 6580 6854 1339 Size: 3484 521 678 411 147 274 2119 Fragments arranged by size: 3484 2119 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1082 1112 4399 4429 1082 Size: 30 3287 30 4287 Fragments arranged by size: 4287 3287 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 754 2216 3197 3502 4324 4435 4498 4519 4561 Size: 1462 981 305 822 111 63 21 42 Cuts at: 4561 754 Size: 3827 Fragments arranged by size: 3827 1462 981 822 305 111 63 42 21 BseYI C'CCAG_C Cuts at: 318 1845 3069 5059 5817 6482 6890 318 Size: 1527 1224 1990 758 665 408 1062 Fragments arranged by size: 1990 1527 1224 1062 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 3429 3575 3836 3429 Size: 146 261 7227 Fragments arranged by size: 7227 261 146 BsiEI CG_ry'CG Cuts at: 104 5176 5222 6452 104 Size: 5072 46 1230 1286 Fragments arranged by size: 5072 1286 1230 46 BsiHKAI G_wGCw'C Cuts at: 149 1535 1617 2267 2321 4648 5426 5616 6476 Size: 1386 82 650 54 2327 778 190 860 Cuts at: 6476 149 Size: 1307 Fragments arranged by size: 2327 1386 1307 860 778 650 190 82 54 BsmI GAATG_Cn' Cuts at: 369 381 1754 3406 3627 369 Size: 12 1373 1652 221 4376 Fragments arranged by size: 4376 1652 1373 221 12 Bsp1286I G_dGCh'C Cuts at: 149 404 516 1535 1617 2267 2321 2376 4648 Size: 255 112 1019 82 650 54 55 2272 Cuts at: 4648 5259 5352 5426 5616 5678 6476 149 Size: 611 93 74 190 62 798 1307 Fragments arranged by size: 2272 1307 1019 798 650 611 255 190 112 93 82 74 62 55 54 BspHI T'CATG_A Cuts at: 359 1800 6066 359 Size: 1441 4266 1927 Fragments arranged by size: 4266 1927 1441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1675 2741 3190 3406 3451 4684 4898 5200 5581 Size: 1066 449 216 45 1233 214 302 381 Cuts at: 5581 1675 Size: 3728 Fragments arranged by size: 3728 1233 1066 449 381 302 216 214 45 BsrBI CCG'CTC Cuts at: 50 3423 3848 3985 5926 5980 7464 7629 50 Size: 3373 425 137 1941 54 1484 165 55 Fragments arranged by size: 3373 1941 1484 425 165 137 55 54 BsrDI GCAATG_nn' Cuts at: 584 2831 3015 3701 5546 584 Size: 2247 184 686 1845 2672 Fragments arranged by size: 2672 2247 1845 686 184 BsrFI r'CCGG_y Cuts at: 1339 5632 5813 1339 Size: 4293 181 3160 Fragments arranged by size: 4293 3160 181 BsrGI T'GTAC_A Cuts at: 4180 4180 Size: 7634 BssSI C'ACGA_G Cuts at: 5905 6613 5905 Size: 708 6926 BstAPI GCAn_nnn'nTGC Cuts at: 5011 5011 Size: 7634 BstBI TT'CG_AA Cuts at: 636 3954 5995 636 Size: 3318 2041 2275 Fragments arranged by size: 3318 2275 2041 BstXI CCAn_nnnn'nTGG Cuts at: 1713 3913 1713 Size: 2200 5434 BstYI r'GATC_y Cuts at: 1 264 821 2059 2462 2633 3759 4659 5126 Size: 263 557 1238 403 171 1126 900 467 Cuts at: 5126 5149 5484 5730 6036 6048 6134 7186 1 Size: 23 335 246 306 12 86 1052 449 Fragments arranged by size: 1238 1126 1052 900 557 467 449 403 335 306 263 246 171 86 23 12 Bsu36I CC'TnA_GG Cuts at: 884 884 Size: 7634 BtgI C'CryG_G Cuts at: 700 1385 2905 3048 700 Size: 685 1520 143 5286 Fragments arranged by size: 5286 1520 685 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 5584 5765 5928 5584 Size: 181 163 7290 Fragments arranged by size: 7290 181 163 BtsI GCAGTG_nn' Cuts at: 172 192 413 2265 2511 2777 4877 172 Size: 20 221 1852 246 266 2100 2929 Fragments arranged by size: 2929 2100 1852 266 246 221 20 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1260 1295 1260 Size: 35 7599 DraI TTT'AAA Cuts at: 410 4633 410 Size: 4223 3411 DrdI GACnn_nn'nnGTC Cuts at: 293 5340 6684 293 Size: 5047 1344 1243 Fragments arranged by size: 5047 1344 1243 EaeI y'GGCC_r Cuts at: 1703 2273 5219 5393 5784 5811 7156 1703 Size: 570 2946 174 391 27 1345 2181 Fragments arranged by size: 2946 2181 1345 570 391 174 27 EagI C'GGCC_G Cuts at: 5219 5219 Size: 7634 EarI CTCTTCn'nnn_ Cuts at: 242 346 717 4070 4301 4316 5657 5867 242 Size: 104 371 3353 231 15 1341 210 2009 Fragments arranged by size: 3353 2009 1341 371 231 210 104 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3326 4426 5842 6584 6730 7444 3326 Size: 1100 1416 742 146 714 3516 Fragments arranged by size: 3516 1416 1100 742 714 146 EcoICRI GAG'CTC Cuts at: 147 1533 4646 147 Size: 1386 3113 3135 Fragments arranged by size: 3135 3113 1386 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 181 266 830 1213 1454 1720 1892 2005 2137 Size: 85 564 383 241 266 172 113 132 Cuts at: 2137 3478 3940 4426 5459 5891 6244 6906 181 Size: 1341 462 486 1033 432 353 662 909 Fragments arranged by size: 1341 1033 909 662 564 486 462 432 383 353 266 241 172 132 113 85 EcoNI CCTnn'n_nnAGG Cuts at: 4501 4501 Size: 7634 EcoO109I rG'GnC_Cy Cuts at: 2373 3548 3948 4773 2373 Size: 1175 400 825 5234 Fragments arranged by size: 5234 1175 825 400 EcoRI G'AATT_C Cuts at: 4638 4638 Size: 7634 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2674 3648 5653 2674 Size: 974 2005 4655 Fragments arranged by size: 4655 2005 974 FspI TGC'GCA Cuts at: 1286 1681 5415 1286 Size: 395 3734 3505 Fragments arranged by size: 3734 3505 395 HaeII r_GCGC'y Cuts at: 3247 5068 5316 6546 7317 3247 Size: 1821 248 1230 771 3564 Fragments arranged by size: 3564 1821 1230 771 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1082 1114 2044 2051 2076 2083 2983 3015 3832 Size: 32 930 7 25 7 900 32 817 Cuts at: 3832 3864 4516 4548 5476 5506 5508 5538 1082 Size: 32 652 32 928 30 2 30 3178 Fragments arranged by size: 3178 930 928 900 817 652 32 32 32 32 30 30 25 7 7 2 HincII GTy'rAC Cuts at: 3743 4673 3743 Size: 930 6704 HindIII A'AGCT_T Cuts at: 803 803 Size: 7634 KasI G'GCGC_C Cuts at: 5064 5312 5064 Size: 248 7386 KpnI G_GTAC'C Cuts at: 205 4654 205 Size: 4449 3185 MfeI C'AATT_G Cuts at: 226 1932 2166 226 Size: 1706 234 5694 Fragments arranged by size: 5694 1706 234 MluI A'CGCG_T Cuts at: 7067 7067 Size: 7634 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2027 2324 3023 4397 6393 6577 2027 Size: 297 699 1374 1996 184 3084 Fragments arranged by size: 3084 1996 1374 699 297 184 MscI TGG'CCA Cuts at: 1705 2275 5395 1705 Size: 570 3120 3944 Fragments arranged by size: 3944 3120 570 MslI CAynn'nnrTG Cuts at: 185 1147 2314 2341 3713 3911 4153 5623 5750 Size: 962 1167 27 1372 198 242 1470 127 Cuts at: 5750 185 Size: 2069 Fragments arranged by size: 2069 1470 1372 1167 962 242 198 127 27 MspA1I CmG'CkG Cuts at: 618 1870 2456 2472 3087 3456 4745 5059 5419 Size: 1252 586 16 615 369 1289 314 360 Cuts at: 5419 6203 6448 618 Size: 784 245 1804 Fragments arranged by size: 1804 1289 1252 784 615 586 369 360 314 245 16 NaeI GCC'GGC Cuts at: 5815 5815 Size: 7634 NarI GG'CG_CC Cuts at: 5065 5313 5065 Size: 248 7386 NcoI C'CATG_G Cuts at: 1385 3048 1385 Size: 1663 5971 NdeI CA'TA_TG Cuts at: 381 381 Size: 7634 NgoMIV G'CCGG_C Cuts at: 5813 5813 Size: 7634 NheI G'CTAG_C Cuts at: 1642 2925 4488 1642 Size: 1283 1563 4788 Fragments arranged by size: 4788 1563 1283 NsiI A_TGCA'T Cuts at: 381 1406 381 Size: 1025 6609 NspI r_CATG'y Cuts at: 130 993 1404 1790 3157 4687 5187 6790 130 Size: 863 411 386 1367 1530 500 1603 974 Fragments arranged by size: 1603 1530 1367 974 863 500 411 386 PasI CC'CwG_GG Cuts at: 1034 2916 1034 Size: 1882 5752 PciI A'CATG_T Cuts at: 126 6786 126 Size: 6660 974 PflMI CCAn_nnn'nTGG Cuts at: 2282 3295 4404 2282 Size: 1013 1109 5512 Fragments arranged by size: 5512 1109 1013 PfoI T'CCnGG_A Cuts at: 3365 3365 Size: 7634 PmeI GTTT'AAAC Cuts at: 4633 4633 Size: 7634 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 2130 6080 6112 2098 Size: 32 3950 32 3620 Fragments arranged by size: 3950 3620 32 32 PpuMI rG'GwC_Cy Cuts at: 3548 3948 3548 Size: 400 7234 PspOMI G'GGCC_C Cuts at: 512 2372 512 Size: 1860 5774 PstI C_TGCA'G Cuts at: 420 2495 2606 4681 5366 420 Size: 2075 111 2075 685 2688 Fragments arranged by size: 2688 2075 2075 685 111 PvuI CG_AT'CG Cuts at: 104 104 Size: 7634 PvuII CAG'CTG Cuts at: 618 1870 2456 2472 3087 3456 5059 5419 618 Size: 1252 586 16 615 369 1603 360 2833 Fragments arranged by size: 2833 1603 1252 615 586 369 360 16 RsrII CG'GwC_CG Cuts at: 5829 5829 Size: 7634 SacI G_AGCT'C Cuts at: 149 1535 4648 149 Size: 1386 3113 3135 Fragments arranged by size: 3135 3113 1386 SalI G'TCGA_C Cuts at: 4671 4671 Size: 7634 SapI GCTCTTCn'nnn_ Cuts at: 5657 5867 5657 Size: 210 7424 SbfI CC_TGCA'GG Cuts at: 4681 4681 Size: 7634 ScaI AGT'ACT Cuts at: 1581 7151 1581 Size: 5570 2064 SfcI C'TryA_G Cuts at: 33 113 416 597 652 658 773 2063 2075 Size: 80 303 181 55 6 115 1290 12 Cuts at: 2075 2491 2602 3057 3803 4139 4677 5362 6330 Size: 416 111 455 746 336 538 685 968 Cuts at: 6330 6521 33 Size: 191 1146 Fragments arranged by size: 1290 1146 968 746 685 538 455 416 336 303 191 181 115 111 80 55 12 6 SfiI GGCCn_nnn'nGGCC Cuts at: 520 520 Size: 7634 SfoI GGC'GCC Cuts at: 5066 5314 5066 Size: 248 7386 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 7634 SmaI CCC'GGG Cuts at: 4656 4656 Size: 7634 SmlI C'TyrA_G Cuts at: 4789 5704 6418 6680 4789 Size: 915 714 262 5743 Fragments arranged by size: 5743 915 714 262 SpeI A'CTAG_T Cuts at: 67 853 67 Size: 786 6848 SphI G_CATG'C Cuts at: 1404 1790 4687 5187 1404 Size: 386 2897 500 3851 Fragments arranged by size: 3851 2897 500 386 SspI AAT'ATT Cuts at: 1105 1637 1105 Size: 532 7102 StyI C'CwwG_G Cuts at: 1385 3048 4768 5122 1385 Size: 1663 1720 354 3897 Fragments arranged by size: 3897 1720 1663 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1026 1781 2854 5217 5350 5809 6018 1026 Size: 755 1073 2363 133 459 209 2642 Fragments arranged by size: 2642 2363 1073 755 459 209 133 TatI w'GTAC_w Cuts at: 1296 1579 4180 7149 1296 Size: 283 2601 2969 1781 Fragments arranged by size: 2969 2601 1781 283 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1734 1766 3783 3815 3845 3877 3908 3940 7151 Size: 32 2017 32 30 32 31 32 3211 Cuts at: 7151 7183 1734 Size: 32 2185 Fragments arranged by size: 3211 2185 2017 32 32 32 32 32 31 30 Tth111I GACn'n_nGTC Cuts at: 2512 5431 2512 Size: 2919 4715 XbaI T'CTAG_A Cuts at: 4500 4665 4500 Size: 165 7469 XcmI CCAnnnn_n'nnnnTGG Cuts at: 957 5129 957 Size: 4172 3462 XmaI C'CCGG_G Cuts at: 4654 4654 Size: 7634 XmnI GAAnn'nnTTC Cuts at: 502 502 Size: 7634 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI AloI AscI AvrII BaeI BcgI BsiWI BsmBI BspEI BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HpaI NotI NruI PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI