Restriction map for
pF1KA0802
(Circular) (Six-base) MAPSORT of: pF1KA0802.seq Check: 402 from: 1 to: 7748
pF1KA0802 7748 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 947 1692 3598 3683 947
Size: 745 1906 85 5012
Fragments arranged by size:
5012 1906 745 85
AatII G_ACGT'C
Cuts at: 1384 4216 1384
Size: 2832 4916
AccI GT'mk_AC
Cuts at: 3590 4786 3590
Size: 1196 6552
Acc65I G'GTAC_C
Cuts at: 3449 4764 3449
Size: 1315 6433
AclI AA'CG_TT
Cuts at: 2906 7588 2906
Size: 4682 3066
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1141 1462 1968 3037 3998 5573 6005 6358
Size: 393 321 506 1069 961 1575 432 353
Cuts at: 6358 7020 748
Size: 662 1476
Fragments arranged by size:
1575 1476 1069 961 662 506 432 393
353 321
AfeI AGC'GCT
Cuts at: 1210 1210
Size: 7748
AflII C'TTAA_G
Cuts at: 343 343
Size: 7748
AflIII A'CryG_T
Cuts at: 5100 6900 7181 5100
Size: 1800 281 5667
Fragments arranged by size:
5667 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1836 1836
Size: 7748
AleI CACnn'nnGTG
Cuts at: 2306 3359 4522 2306
Size: 1053 1163 5532
Fragments arranged by size:
5532 1163 1053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2030 2543 2030
Size: 513 7235
AlwNI CAG_nnn'CTG
Cuts at: 1641 2439 2977 3519 5053 6491 1641
Size: 798 538 542 1534 1438 2898
Fragments arranged by size:
2898 1534 1438 798 542 538
ApaI G_GGCC'C
Cuts at: 704 859 1104 1828 4260 4426 4646 4700 704
Size: 155 245 724 2432 166 220 54 3752
Fragments arranged by size:
3752 2432 724 245 220 166 155 54
ApaLI G'TGCA_C
Cuts at: 2671 4288 6586 2671
Size: 1617 2298 3833
Fragments arranged by size:
3833 2298 1617
ApoI r'AATT_y
Cuts at: 2821 4752 7101 7357 2821
Size: 1931 2349 256 3212
Fragments arranged by size:
3212 2349 1931 256
AseI AT'TA_AT
Cuts at: 20 4301 20
Size: 4281 3467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7748
AvaI C'yCGr_G
Cuts at: 488 713 858 1049 1253 1448 1787 1870 1880
Size: 225 145 191 204 195 339 83 10
Cuts at: 1880 2254 2354 3109 3651 3722 4451 4647 4768
Size: 374 100 755 542 71 729 196 121
Cuts at: 4768 488
Size: 3468
Fragments arranged by size:
3468 755 729 542 374 339 225 204
196 195 191 145 121 100 83 71
10
BamHI G'GATC_C
Cuts at: 4580 4773 4580
Size: 193 7555
BanI G'GyrC_C
Cuts at: 545 1087 3449 3746 4441 4764 5178 5426 5461
Size: 542 2362 297 695 323 414 248 35
Cuts at: 5461 545
Size: 2832
Fragments arranged by size:
2832 2362 695 542 414 323 297 248
35
BanII G_rGCy'C
Cuts at: 638 704 859 929 1025 1104 1151 1439 1634
Size: 66 155 70 96 79 47 288 195
Cuts at: 1634 1828 2117 3652 4260 4285 4409 4426 4646
Size: 194 289 1535 608 25 124 17 220
Cuts at: 4646 4700 4762 5792 638
Size: 54 62 1030 2594
Fragments arranged by size:
2594 1535 1030 608 289 288 220 195
194 155 124 96 79 70 66 62
54 47 25 17
BbeI G_GCGC'C
Cuts at: 3750 5182 5430 3750
Size: 1432 248 6068
Fragments arranged by size:
6068 1432 248
BbsI GAAGACnn'nnnn_
Cuts at: 208 1001 1384 2605 3002 208
Size: 793 383 1221 397 4954
Fragments arranged by size:
4954 1221 793 397 383
BbvCI CC'TCA_GC
Cuts at: 374 374
Size: 7748
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 145 179 1258 1292 145
Size: 34 1079 34 6601
Fragments arranged by size:
6601 1079 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5646 6702 7748 5646
Size: 1056 1046 5646
Fragments arranged by size:
5646 1056 1046
BclI T'GATC_A
Cuts at: 2889 5268 2889
Size: 2379 5369
BglI GCCn_nnn'nGGC
Cuts at: 1062 1595 2455 1062
Size: 533 860 6355
Fragments arranged by size:
6355 860 533
BglII A'GATC_T
Cuts at: 5263 7300 5263
Size: 2037 5711
BlpI GC'TnA_GC
Cuts at: 1024 1150 3688 4398 4860 1024
Size: 126 2538 710 462 3912
Fragments arranged by size:
3912 2538 710 462 126
Bme1580I G_kGCm'C
Cuts at: 704 859 1104 1245 1828 2505 2675 3413 3744
Size: 155 245 141 583 677 170 738 331
Cuts at: 3744 4260 4292 4426 4519 4646 4700 5373 5466
Size: 516 32 134 93 127 54 673 93
Cuts at: 5466 6590 704
Size: 1124 1862
Fragments arranged by size:
1862 1124 738 677 673 583 516 331
245 170 155 141 134 127 93 93
54 32
BmgBI CAC'GTC
Cuts at: 2553 3531 2553
Size: 978 6770
BmrI ACTGGGnnnn_n'
Cuts at: 1241 4960 5041 5376 7520 1241
Size: 3719 81 335 2144 1469
Fragments arranged by size:
3719 2144 1469 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1374 2262 6115 1374
Size: 888 3853 3007
Fragments arranged by size:
3853 3007 888
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 421 469 538 622 646 811 931 1576
Size: 111 48 69 84 24 165 120 645
Cuts at: 1576 1717 1879 2011 2103 2232 2485 3275 3659
Size: 141 162 132 92 129 253 790 384
Cuts at: 3659 3826 4299 310
Size: 167 473 3759
Fragments arranged by size:
3759 790 645 473 384 253 167 165
162 141 132 129 120 111 92 84
69 48 24
Bpu10I CC'TnA_GC
Cuts at: 374 374
Size: 7748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 796 2040 2554 3286 3843 4924 5803 6517 6815
Size: 1244 514 732 557 1081 879 714 298
Cuts at: 6815 796
Size: 1729
Fragments arranged by size:
1729 1244 1081 879 732 714 557 514
298
BsaI GGTCTCn'nnnn_
Cuts at: 2233 4397 4657 7452 2233
Size: 2164 260 2795 2529
Fragments arranged by size:
2795 2529 2164 260
BsaAI yAC'GTr
Cuts at: 5101 5101
Size: 7748
BsaBI GATnn'nnATC
Cuts at: 6 5262 7299 6
Size: 5256 2037 455
Fragments arranged by size:
5256 2037 455
BsaHI Gr'CG_yC
Cuts at: 1381 3747 4213 5179 5427 6129 7625 1381
Size: 2366 466 966 248 702 1496 1504
Fragments arranged by size:
2366 1504 1496 966 702 466 248
BsaWI w'CCGG_w
Cuts at: 971 2117 4937 5458 6136 6547 6694 6968 971
Size: 1146 2820 521 678 411 147 274 1751
Fragments arranged by size:
2820 1751 1146 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 419 449 1108 1138 1354 1384 2104 2134 419
Size: 30 659 30 216 30 720 30 6033
Fragments arranged by size:
6033 720 659 216 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 766 1129 1318 1378 1618 2014 2017 2215 2227
Size: 363 189 60 240 396 3 198 12
Cuts at: 2227 2619 3328 3432 4633 4638 766
Size: 392 709 104 1201 5 3876
Fragments arranged by size:
3876 1201 709 396 392 363 240 198
189 104 60 12 5 3
BseYI C'CCAG_C
Cuts at: 706 877 1319 1490 1634 1641 1821 1965 3329
Size: 171 442 171 144 7 180 144 1364
Cuts at: 3329 4036 4419 4674 5173 5931 6596 7004 706
Size: 707 383 255 499 758 665 408 1450
Fragments arranged by size:
1450 1364 758 707 665 499 442 408
383 255 180 171 171 144 144 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1912 2380 2990 3781 4135 4372 4515 1912
Size: 468 610 791 354 237 143 5145
Fragments arranged by size:
5145 791 610 468 354 237 143
BsiEI CG_ry'CG
Cuts at: 104 2778 3004 5290 5336 6566 104
Size: 2674 226 2286 46 1230 1286
Fragments arranged by size:
2674 2286 1286 1230 226 46
BsiHKAI G_wGCw'C
Cuts at: 638 929 1025 1151 1166 1439 2009 2117 2342
Size: 291 96 126 15 273 570 108 225
Cuts at: 2342 2570 2675 3037 3503 4292 4762 5540 5730
Size: 228 105 362 466 789 470 778 190
Cuts at: 5730 6590 638
Size: 860 1796
Fragments arranged by size:
1796 860 789 778 570 470 466 362
291 273 228 225 190 126 108 105
96 15
BsmI GAATG_Cn'
Cuts at: 881 3867 881
Size: 2986 4762
BsmBI CGTCTCn'nnnn_
Cuts at: 247 4067 247
Size: 3820 3928
Bsp1286I G_dGCh'C
Cuts at: 638 704 859 929 1025 1104 1151 1166 1245
Size: 66 155 70 96 79 47 15 79
Cuts at: 1245 1439 1634 1828 2009 2117 2342 2505 2570
Size: 194 195 194 181 108 225 163 65
Cuts at: 2570 2675 3037 3413 3503 3652 3744 4260 4285
Size: 105 362 376 90 149 92 516 25
Cuts at: 4285 4292 4409 4426 4519 4646 4700 4762 5373
Size: 7 117 17 93 127 54 62 611
Cuts at: 5373 5466 5540 5730 5792 6590 638
Size: 93 74 190 62 798 1796
Fragments arranged by size:
1796 798 611 516 376 362 225 195
194 194 190 181 163 155 149 127
117 108 105 96 93 93 92 90
79 79 74 70 66 65 62 62
54 47 25 17 15 7
BspEI T'CCGG_A
Cuts at: 971 971
Size: 7748
BspHI T'CATG_A
Cuts at: 6180 6180
Size: 7748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 947 1692 1987 2723 3598 3683 4798 5012 5314
Size: 745 295 736 875 85 1115 214 302
Cuts at: 5314 5695 947
Size: 381 3000
Fragments arranged by size:
3000 1115 875 745 736 381 302 295
214 85
BsrBI CCG'CTC
Cuts at: 50 849 1397 1503 1608 1893 2808 4262 6040
Size: 799 548 106 105 285 915 1454 1778
Cuts at: 6040 6094 7578 7743 50
Size: 54 1484 165 55
Fragments arranged by size:
1778 1484 1454 915 799 548 285 165
106 105 55 54
BsrDI GCAATG_nn'
Cuts at: 5660 5660
Size: 7748
BsrFI r'CCGG_y
Cuts at: 1004 1875 2999 4381 5746 5927 1004
Size: 871 1124 1382 1365 181 2825
Fragments arranged by size:
2825 1382 1365 1124 871 181
BsrGI T'GTAC_A
Cuts at: 497 497
Size: 7748
BssHII G'CGCG_C
Cuts at: 363 363
Size: 7748
BssSI C'ACGA_G
Cuts at: 1018 1177 1483 3412 6019 6727 1018
Size: 159 306 1929 2607 708 2039
Fragments arranged by size:
2607 2039 1929 708 306 159
BstAPI GCAn_nnn'nTGC
Cuts at: 3519 4139 5125 3519
Size: 620 986 6142
Fragments arranged by size:
6142 986 620
BstBI TT'CG_AA
Cuts at: 282 6109 282
Size: 5827 1921
BstEII G'GTnAC_C
Cuts at: 2278 2278
Size: 7748
BstXI CCAn_nnnn'nTGG
Cuts at: 768 2290 3361 3919 3963 4479 768
Size: 1522 1071 558 44 516 4037
Fragments arranged by size:
4037 1522 1071 558 516 44
BstYI r'GATC_y
Cuts at: 1 2726 4580 4773 5240 5263 5598 5844 6150
Size: 2725 1854 193 467 23 335 246 306
Cuts at: 6150 6162 6248 7300 1
Size: 12 86 1052 449
Fragments arranged by size:
2725 1854 1052 467 449 335 306 246
193 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3137 4078 3137
Size: 941 6807
BtgI C'CryG_G
Cuts at: 107 683 804 1430 1959 2393 3476 4346 4638
Size: 576 121 626 529 434 1083 870 292
Cuts at: 4638 4700 107
Size: 62 3155
Fragments arranged by size:
3155 1083 870 626 576 529 434 292
121 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1277 5698 5879 6042 1277
Size: 4421 181 163 2983
Fragments arranged by size:
4421 2983 181 163
BtsI GCAGTG_nn'
Cuts at: 3501 3517 3793 4991 3501
Size: 16 276 1198 6258
Fragments arranged by size:
6258 1198 276 16
DraI TTT'AAA
Cuts at: 4747 4747
Size: 7748
DrdI GACnn_nn'nnGTC
Cuts at: 1782 3921 5454 6798 1782
Size: 2139 1533 1344 2732
Fragments arranged by size:
2732 2139 1533 1344
EaeI y'GGCC_r
Cuts at: 596 2954 3001 3038 5333 5507 5898 5925 7270
Size: 2358 47 37 2295 174 391 27 1345
Cuts at: 7270 596
Size: 1074
Fragments arranged by size:
2358 2295 1345 1074 391 174 47 37
27
EagI C'GGCC_G
Cuts at: 3001 5333 3001
Size: 2332 5416
EarI CTCTTCn'nnn_
Cuts at: 107 947 1595 2149 2588 3083 4327 5771 5981
Size: 840 648 554 439 495 1244 1444 210
Cuts at: 5981 107
Size: 1874
Fragments arranged by size:
1874 1444 1244 840 648 554 495 439
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 919 2302 5956 6698 6844 7558 919
Size: 1383 3654 742 146 714 1109
Fragments arranged by size:
3654 1383 1109 742 714 146
EcoICRI GAG'CTC
Cuts at: 636 927 1023 1149 1437 2115 4760 636
Size: 291 96 126 288 678 2645 3624
Fragments arranged by size:
3624 2645 678 291 288 126 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 421 469 538 622 646 748 811 931
Size: 111 48 69 84 24 102 63 120
Cuts at: 931 1141 1462 1576 1717 1879 1968 2011 2103
Size: 210 321 114 141 162 89 43 92
Cuts at: 2103 2232 2485 3037 3275 3659 3826 3998 4299
Size: 129 253 552 238 384 167 172 301
Cuts at: 4299 5573 6005 6358 7020 310
Size: 1274 432 353 662 1038
Fragments arranged by size:
1274 1038 662 552 432 384 353 321
301 253 238 210 172 167 162 141
129 120 114 111 102 92 89 84
69 63 48 43 24
EcoNI CCTnn'n_nnAGG
Cuts at: 292 1111 1699 1813 3136 3711 292
Size: 819 588 114 1323 575 4329
Fragments arranged by size:
4329 1323 819 588 575 114
EcoO109I rG'GnC_Cy
Cuts at: 432 701 855 1100 1101 1336 1582 2648 3243
Size: 269 154 245 1 235 246 1066 595
Cuts at: 3243 3619 3670 3923 3938 4356 4643 4696 4887
Size: 376 51 253 15 418 287 53 191
Cuts at: 4887 432
Size: 3293
Fragments arranged by size:
3293 1066 595 418 376 287 269 253
246 245 235 191 154 53 51 15
1
EcoRI G'AATT_C
Cuts at: 2821 4752 2821
Size: 1931 5817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 531 2667 2841 3181 4551 5767 531
Size: 2136 174 340 1370 1216 2512
Fragments arranged by size:
2512 2136 1370 1216 340 174
FseI GG_CCGG'CC
Cuts at: 1879 1879
Size: 7748
FspI TGC'GCA
Cuts at: 586 3583 5529 586
Size: 2997 1946 2805
Fragments arranged by size:
2997 2805 1946
HaeII r_GCGC'y
Cuts at: 1212 1518 2450 3750 5182 5430 6660 7431 1212
Size: 306 932 1300 1432 248 1230 771 1529
Fragments arranged by size:
1529 1432 1300 1230 932 771 306 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1108 1140 1366 1398 1711 1743 1822 1854 2104
Size: 32 226 32 313 32 79 32 250
Cuts at: 2104 2136 2355 2387 2965 2997 3019 3051 3052
Size: 32 219 32 578 32 22 32 1
Cuts at: 3052 3084 4297 4329 4654 4686 5590 5620 5622
Size: 32 1213 32 325 32 904 30 2
Cuts at: 5622 5652 1108
Size: 30 3204
Fragments arranged by size:
3204 1213 904 578 325 313 250 226
219 79 32 32 32 32 32 32
32 32 32 32 32 30 30 22
2 1
HincII GTy'rAC
Cuts at: 3591 4787 3591
Size: 1196 6552
KasI G'GCGC_C
Cuts at: 3746 5178 5426 3746
Size: 1432 248 6068
Fragments arranged by size:
6068 1432 248
KpnI G_GTAC'C
Cuts at: 3453 4768 3453
Size: 1315 6433
MluI A'CGCG_T
Cuts at: 7181 7181
Size: 7748
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 420 1223 3005 6507 6691 420
Size: 803 1782 3502 184 1477
Fragments arranged by size:
3502 1782 1477 803 184
MscI TGG'CCA
Cuts at: 598 2956 5509 598
Size: 2358 2553 2837
Fragments arranged by size:
2837 2553 2358
MslI CAynn'nnrTG
Cuts at: 2012 2306 3359 3961 4477 4522 5737 5864 2012
Size: 294 1053 602 516 45 1215 127 3896
Fragments arranged by size:
3896 1215 1053 602 516 294 127 45
MspA1I CmG'CkG
Cuts at: 153 1154 1494 1638 1857 2395 2439 2589 2635
Size: 1001 340 144 219 538 44 150 46
Cuts at: 2635 3006 3995 4859 5173 5533 6317 6562 153
Size: 371 989 864 314 360 784 245 1339
Fragments arranged by size:
1339 1001 989 864 784 538 371 360
340 314 245 219 150 144 46 44
NaeI GCC'GGC
Cuts at: 1877 4383 5929 1877
Size: 2506 1546 3696
Fragments arranged by size:
3696 2506 1546
NarI GG'CG_CC
Cuts at: 3747 5179 5427 3747
Size: 1432 248 6068
Fragments arranged by size:
6068 1432 248
NcoI C'CATG_G
Cuts at: 107 4700 107
Size: 4593 3155
NgoMIV G'CCGG_C
Cuts at: 1875 4381 5927 1875
Size: 2506 1546 3696
Fragments arranged by size:
3696 2506 1546
NspI r_CATG'y
Cuts at: 834 2943 4139 4609 4801 5301 6904 834
Size: 2109 1196 470 192 500 1603 1678
Fragments arranged by size:
2109 1678 1603 1196 500 470 192
PasI CC'CwG_GG
Cuts at: 3777 4733 3777
Size: 956 6792
PciI A'CATG_T
Cuts at: 6900 6900
Size: 7748
PflMI CCAn_nnn'nTGG
Cuts at: 1641 1965 4036 4674 1641
Size: 324 2071 638 4715
Fragments arranged by size:
4715 2071 638 324
PfoI T'CCnGG_A
Cuts at: 1694 3925 4069 1694
Size: 2231 144 5373
Fragments arranged by size:
5373 2231 144
PmeI GTTT'AAAC
Cuts at: 4747 4747
Size: 7748
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3103 3135 3671 3703 6194 6226 3103
Size: 32 536 32 2491 32 4625
Fragments arranged by size:
4625 2491 536 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3619 3923 3938 4356 3619
Size: 304 15 418 7011
Fragments arranged by size:
7011 418 304 15
PspOMI G'GGCC_C
Cuts at: 700 855 1100 1824 4256 4422 4642 4696 700
Size: 155 245 724 2432 166 220 54 3752
Fragments arranged by size:
3752 2432 724 245 220 166 155 54
PstI C_TGCA'G
Cuts at: 158 209 944 1112 1139 1175 1688 2390 2519
Size: 51 735 168 27 36 513 702 129
Cuts at: 2519 4172 4795 5480 158
Size: 1653 623 685 2426
Fragments arranged by size:
2426 1653 735 702 685 623 513 168
129 51 36 27
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7748
PvuII CAG'CTG
Cuts at: 153 1638 1857 2439 2589 5173 5533 153
Size: 1485 219 582 150 2584 360 2368
Fragments arranged by size:
2584 2368 1485 582 360 219 150
RsrII CG'GwC_CG
Cuts at: 5943 5943
Size: 7748
SacI G_AGCT'C
Cuts at: 638 929 1025 1151 1439 2117 4762 638
Size: 291 96 126 288 678 2645 3624
Fragments arranged by size:
3624 2645 678 291 288 126 96
SacII CC_GC'GG
Cuts at: 2396 2396
Size: 7748
SalI G'TCGA_C
Cuts at: 3589 4785 3589
Size: 1196 6552
SanDI GG'GwC_CC
Cuts at: 3619 3923 3938 3619
Size: 304 15 7429
Fragments arranged by size:
7429 304 15
SapI GCTCTTCn'nnn_
Cuts at: 3083 5771 5981 3083
Size: 2688 210 4850
Fragments arranged by size:
4850 2688 210
SbfI CC_TGCA'GG
Cuts at: 1112 4795 1112
Size: 3683 4065
ScaI AGT'ACT
Cuts at: 7265 7265
Size: 7748
SfcI C'TryA_G
Cuts at: 33 154 205 940 1108 1135 1171 1684 2386
Size: 121 51 735 168 27 36 513 702
Cuts at: 2386 2515 4168 4791 5476 6444 6635 33
Size: 129 1653 623 685 968 191 1146
Fragments arranged by size:
1653 1146 968 735 702 685 623 513
191 168 129 121 51 36 27
SfoI GGC'GCC
Cuts at: 3748 5180 5428 3748
Size: 1432 248 6068
Fragments arranged by size:
6068 1432 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7748
SmaI CCC'GGG
Cuts at: 715 860 1051 1255 1450 1789 2356 3653 3724
Size: 145 191 204 195 339 567 1297 71
Cuts at: 3724 4649 4770 715
Size: 925 121 3693
Fragments arranged by size:
3693 1297 925 567 339 204 195 191
145 121 71
SmlI C'TyrA_G
Cuts at: 343 811 2019 2569 3301 3822 4903 5818 6532
Size: 468 1208 550 732 521 1081 915 714
Cuts at: 6532 6794 343
Size: 262 1297
Fragments arranged by size:
1297 1208 1081 915 732 714 550 521
468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7748
SphI G_CATG'C
Cuts at: 2943 4801 5301 2943
Size: 1858 500 5390
Fragments arranged by size:
5390 1858 500
StuI AGG'CCT
Cuts at: 1690 2458 1690
Size: 768 6980
StyI C'CwwG_G
Cuts at: 107 3662 4700 4882 5236 107
Size: 3555 1038 182 354 2619
Fragments arranged by size:
3555 2619 1038 354 182
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 891 2327 3357 3406 4379 5331 5464 5923 6132
Size: 1436 1030 49 973 952 133 459 209
Cuts at: 6132 891
Size: 2507
Fragments arranged by size:
2507 1436 1030 973 952 459 209 133
49
TatI w'GTAC_w
Cuts at: 497 644 3305 7263 497
Size: 147 2661 3958 982
Fragments arranged by size:
3958 2661 982 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2542 2574 2812 2844 7265 7297 2542
Size: 32 238 32 4421 32 2993
Fragments arranged by size:
4421 2993 238 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5545 5545
Size: 7748
XbaI T'CTAG_A
Cuts at: 4779 4779
Size: 7748
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1806 2957 5243 1806
Size: 1151 2286 4311
Fragments arranged by size:
4311 2286 1151
XmaI C'CCGG_G
Cuts at: 713 858 1049 1253 1448 1787 2354 3651 3722
Size: 145 191 204 195 339 567 1297 71
Cuts at: 3722 4647 4768 713
Size: 925 121 3693
Fragments arranged by size:
3693 1297 925 567 339 204 195 191
145 121 71
XmnI GAAnn'nnTTC
Cuts at: 1669 2137 2821 1669
Size: 468 684 6596
Fragments arranged by size:
6596 684 468
ZraI GAC'GTC
Cuts at: 1382 4214 1382
Size: 2832 4916
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AgeI AloI AscI AvrII BaeI BfrBI BmtI BsiWI
BstZ17I ClaI CspCI DraIII EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI