Restriction map for
pF1KA0804
(Circular) (Six-base) MAPSORT of: pF1KA0804.seq Check: 7944 from: 1 to: 7391
pF1KA0804 7391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1439 2156 2784 4429 1439
Size: 717 628 1645 4401
Fragments arranged by size:
4401 1645 717 628
Acc65I G'GTAC_C
Cuts at: 4407 4407
Size: 7391
AclI AA'CG_TT
Cuts at: 7231 7231
Size: 7391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2354 3034 3070 3620 3628 5216 5648 6395 6663
Size: 680 36 550 8 1588 432 747 268
Cuts at: 6663 2354
Size: 3082
Fragments arranged by size:
3082 1588 747 680 550 432 268 36
8
AfeI AGC'GCT
Cuts at: 2063 2063
Size: 7391
AflIII A'CryG_T
Cuts at: 1272 4743 5847 6824 1272
Size: 3471 1104 977 1839
Fragments arranged by size:
3471 1839 1104 977
AgeI A'CCGG_T
Cuts at: 5779 5779
Size: 7391
AhdI GACnn_n'nnGTC
Cuts at: 2731 2731
Size: 7391
AleI CACnn'nnGTG
Cuts at: 1345 1345
Size: 7391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1363 1363
Size: 7391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 229 261 1214 1246 2750 2782 229
Size: 32 953 32 1504 32 4838
Fragments arranged by size:
4838 1504 953 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1494 3567 4171 4696 6263 1494
Size: 2073 604 525 1567 2622
Fragments arranged by size:
2622 2073 1567 604 525
ApaLI G'TGCA_C
Cuts at: 6161 6161
Size: 7391
ApoI r'AATT_y
Cuts at: 211 527 1332 1923 2176 2371 2461 2568 2683
Size: 316 805 591 253 195 90 107 115
Cuts at: 2683 2822 2830 3109 3489 3991 4062 4395 6744
Size: 139 8 279 380 502 71 333 2349
Cuts at: 6744 7000 211
Size: 256 602
Fragments arranged by size:
2349 805 602 591 502 380 333 316
279 256 253 195 139 115 107 90
71 8
AseI AT'TA_AT
Cuts at: 20 2044 20
Size: 2024 5367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7391
AvaI C'yCGr_G
Cuts at: 4411 4411
Size: 7391
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3446 3479 3446
Size: 33 7358
BamHI G'GATC_C
Cuts at: 363 1140 3429 3687 4140 4179 4416 363
Size: 777 2289 258 453 39 237 3338
Fragments arranged by size:
3338 2289 777 453 258 237 39
BanI G'GyrC_C
Cuts at: 1842 3352 4407 4821 5069 5104 1842
Size: 1510 1055 414 248 35 4129
Fragments arranged by size:
4129 1510 1055 414 248 35
BanII G_rGCy'C
Cuts at: 1782 4405 5435 1782
Size: 2623 1030 3738
Fragments arranged by size:
3738 2623 1030
BbeI G_GCGC'C
Cuts at: 4825 5073 4825
Size: 248 7143
BbsI GAAGACnn'nnnn_
Cuts at: 3115 3115
Size: 7391
BbvCI CC'TCA_GC
Cuts at: 1591 1591
Size: 7391
BciVI GTATCCnnnnn_n'
Cuts at: 603 5289 6050 7391 603
Size: 4686 761 1341 603
Fragments arranged by size:
4686 1341 761 603
BclI T'GATC_A
Cuts at: 642 2670 4203 4911 642
Size: 2028 1533 708 3122
Fragments arranged by size:
3122 2028 1533 708
BglII A'GATC_T
Cuts at: 1418 1550 2337 3804 4906 6943 1418
Size: 132 787 1467 1102 2037 1866
Fragments arranged by size:
2037 1866 1467 1102 787 132
BlpI GC'TnA_GC
Cuts at: 4503 4503
Size: 7391
Bme1580I G_kGCm'C
Cuts at: 1795 5016 5109 6165 1795
Size: 3221 93 1056 3021
Fragments arranged by size:
3221 3021 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 4603 4684 5019 7163 4603
Size: 81 335 2144 4831
Fragments arranged by size:
4831 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3450 5758 3450
Size: 2308 5083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 812 1283 1493 1620 2777 4218 262
Size: 550 471 210 127 1157 1441 3435
Fragments arranged by size:
3435 1441 1157 550 471 210 127
Bpu10I CC'TnA_GC
Cuts at: 1591 1591
Size: 7391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 272 379 720 1978 3274 4567 5446 5938 6236
Size: 107 341 1258 1296 1293 879 492 298
Cuts at: 6236 272
Size: 1427
Fragments arranged by size:
1427 1296 1293 1258 879 492 341 298
107
BsaI GGTCTCn'nnnn_
Cuts at: 3460 7095 3460
Size: 3635 3756
BsaAI yAC'GTr
Cuts at: 2884 4744 2884
Size: 1860 5531
BsaBI GATnn'nnATC
Cuts at: 6 647 821 4905 6942 6
Size: 641 174 4084 2037 455
Fragments arranged by size:
4084 2037 641 455 174
BsaHI Gr'CG_yC
Cuts at: 3903 4822 5070 5772 7268 3903
Size: 919 248 702 1496 4026
Fragments arranged by size:
4026 1496 919 702 248
BsaWI w'CCGG_w
Cuts at: 1742 2792 4580 5101 5779 6053 6200 6611 1742
Size: 1050 1788 521 678 274 147 411 2522
Fragments arranged by size:
2522 1788 1050 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 887 917 1214 1244 1466 1496 2692 2722 2750
Size: 30 297 30 222 30 1196 30 28
Cuts at: 2750 2780 2950 2980 887
Size: 30 170 30 5298
Fragments arranged by size:
5298 1196 297 222 170 30 30 30
30 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 290 1235 1608 2207 2210 2971 3132 4237 290
Size: 945 373 599 3 761 161 1105 3444
Fragments arranged by size:
3444 1105 945 761 599 373 161 3
BseYI C'CCAG_C
Cuts at: 4816 5574 6151 6647 4816
Size: 758 577 496 5560
Fragments arranged by size:
5560 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2972 2972
Size: 7391
BsiEI CG_ry'CG
Cuts at: 104 4933 4979 6187 104
Size: 4829 46 1208 1308
Fragments arranged by size:
4829 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1352 1782 4314 4405 5183 5373 6165 1352
Size: 430 2532 91 778 190 792 2578
Fragments arranged by size:
2578 2532 792 778 430 190 91
BsmI GAATG_Cn'
Cuts at: 1005 2232 2388 3985 1005
Size: 1227 156 1597 4411
Fragments arranged by size:
4411 1597 1227 156
BsmBI CGTCTCn'nnnn_
Cuts at: 3894 4222 3894
Size: 328 7063
Bsp1286I G_dGCh'C
Cuts at: 1352 1782 1795 4314 4405 5016 5109 5183 5373
Size: 430 13 2519 91 611 93 74 190
Cuts at: 5373 5435 6165 1352
Size: 62 730 2578
Fragments arranged by size:
2578 2519 730 611 430 190 93 91
74 62 13
BspEI T'CCGG_A
Cuts at: 2792 6611 2792
Size: 3819 3572
BspHI T'CATG_A
Cuts at: 3306 6567 3306
Size: 3261 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2094 2293 2721 4441 4655 4957 5338 2094
Size: 199 428 1720 214 302 381 4147
Fragments arranged by size:
4147 1720 428 381 302 214 199
BsrBI CCG'CTC
Cuts at: 50 5683 5737 7221 7386 50
Size: 5633 54 1484 165 55
Fragments arranged by size:
5633 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2990 3470 3554 5303 2210
Size: 780 480 84 1749 4298
Fragments arranged by size:
4298 1749 780 480 84
BsrFI r'CCGG_y
Cuts at: 5389 5570 5779 5389
Size: 181 209 7001
Fragments arranged by size:
7001 209 181
BsrGI T'GTAC_A
Cuts at: 2070 2070
Size: 7391
BssSI C'ACGA_G
Cuts at: 3004 5662 6020 3004
Size: 2658 358 4375
Fragments arranged by size:
4375 2658 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 3652 4768 2308
Size: 1344 1116 4931
Fragments arranged by size:
4931 1344 1116
BstBI TT'CG_AA
Cuts at: 5752 5752
Size: 7391
BstEII G'GTnAC_C
Cuts at: 3840 3840
Size: 7391
BstXI CCAn_nnnn'nTGG
Cuts at: 3055 3055
Size: 7391
BstYI r'GATC_y
Cuts at: 1 363 1140 1418 1550 1884 2337 3429 3687
Size: 362 777 278 132 334 453 1092 258
Cuts at: 3687 3804 4140 4179 4416 4883 4906 5241 5487
Size: 117 336 39 237 467 23 335 246
Cuts at: 5487 6499 6585 6597 6943 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1092 1012 777 467 453 449 362 346
336 335 334 278 258 246 237 132
117 86 39 23 12
Bsu36I CC'TnA_GG
Cuts at: 2408 2408
Size: 7391
BtgI C'CryG_G
Cuts at: 107 1058 107
Size: 951 6440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5341 5522 5685 5341
Size: 181 163 7047
Fragments arranged by size:
7047 181 163
BtsI GCAGTG_nn'
Cuts at: 614 1156 4145 4634 614
Size: 542 2989 489 3371
Fragments arranged by size:
3371 2989 542 489
DraI TTT'AAA
Cuts at: 2499 3632 4390 2499
Size: 1133 758 5500
Fragments arranged by size:
5500 1133 758
DraIII CAC_nnn'GTG
Cuts at: 776 776
Size: 7391
DrdI GACnn_nn'nnGTC
Cuts at: 5097 5955 5097
Size: 858 6533
EaeI y'GGCC_r
Cuts at: 785 1055 1131 1931 3942 4976 5150 5541 5568
Size: 270 76 800 2011 1034 174 391 27
Cuts at: 5568 6913 785
Size: 1345 1263
Fragments arranged by size:
2011 1345 1263 1034 800 391 270 174
76 27
EagI C'GGCC_G
Cuts at: 4976 4976
Size: 7391
EarI CTCTTCn'nnn_
Cuts at: 164 2771 5414 5624 164
Size: 2607 2643 210 1931
Fragments arranged by size:
2643 2607 1931 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5599 5909 6055 7201 5599
Size: 310 146 1146 5789
Fragments arranged by size:
5789 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1780 4403 1780
Size: 2623 4768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 812 1283 1493 1620 2354 2777 3034 3070
Size: 550 471 210 127 734 423 257 36
Cuts at: 3070 3620 3628 4218 5216 5648 6395 6663 262
Size: 550 8 590 998 432 747 268 990
Fragments arranged by size:
998 990 747 734 590 550 550 471
432 423 268 257 210 127 36 8
EcoO109I rG'GnC_Cy
Cuts at: 2676 2733 3522 4530 2676
Size: 57 789 1008 5537
Fragments arranged by size:
5537 1008 789 57
EcoRI G'AATT_C
Cuts at: 1923 2568 2683 4395 1923
Size: 645 115 1712 4919
Fragments arranged by size:
4919 1712 645 115
EcoRV GAT'ATC
Cuts at: 2082 2082
Size: 7391
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 220 1654 3727 5410 220
Size: 1434 2073 1683 2201
Fragments arranged by size:
2201 2073 1683 1434
FspI TGC'GCA
Cuts at: 5172 5172
Size: 7391
HaeII r_GCGC'y
Cuts at: 704 2065 4825 5073 6095 7074 704
Size: 1361 2760 248 1022 979 1021
Fragments arranged by size:
2760 1361 1022 1021 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 400 417 432 1599 1631 2692 2717 2724
Size: 15 17 15 1167 32 1061 25 7
Cuts at: 2724 2749 2950 2982 3459 3491 3543 3575 3824
Size: 25 201 32 477 32 52 32 249
Cuts at: 3824 3856 3859 3891 5233 5263 5265 5295 385
Size: 32 3 32 1342 30 2 30 2481
Fragments arranged by size:
2481 1342 1167 1061 477 249 201 52
32 32 32 32 32 32 30 30
25 25 17 15 15 7 3 2
HincII GTy'rAC
Cuts at: 2785 4430 2785
Size: 1645 5746
HindIII A'AGCT_T
Cuts at: 197 653 197
Size: 456 6935
KasI G'GCGC_C
Cuts at: 4821 5069 4821
Size: 248 7143
KpnI G_GTAC'C
Cuts at: 4411 4411
Size: 7391
MfeI C'AATT_G
Cuts at: 584 584
Size: 7391
MluI A'CGCG_T
Cuts at: 6824 6824
Size: 7391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 328 666 892 1485 6062 6246 328
Size: 338 226 593 4577 184 1473
Fragments arranged by size:
4577 1473 593 338 226 184
MscI TGG'CCA
Cuts at: 787 1057 1933 3944 5152 787
Size: 270 876 2011 1208 3026
Fragments arranged by size:
3026 2011 1208 876 270
MslI CAynn'nnrTG
Cuts at: 131 226 404 1345 2794 2855 3410 5380 5507
Size: 95 178 941 1449 61 555 1970 127
Cuts at: 5507 131
Size: 2015
Fragments arranged by size:
2015 1970 1449 941 555 178 127 95
61
MspA1I CmG'CkG
Cuts at: 553 893 1434 4353 4502 4816 5176 6189 6434
Size: 340 541 2919 149 314 360 1013 245
Cuts at: 6434 553
Size: 1510
Fragments arranged by size:
2919 1510 1013 541 360 340 314 245
149
NaeI GCC'GGC
Cuts at: 5572 5572
Size: 7391
NarI GG'CG_CC
Cuts at: 4822 5070 4822
Size: 248 7143
NcoI C'CATG_G
Cuts at: 107 1058 107
Size: 951 6440
NdeI CA'TA_TG
Cuts at: 2075 2075
Size: 7391
NgoMIV G'CCGG_C
Cuts at: 5570 5570
Size: 7391
NspI r_CATG'y
Cuts at: 1276 1378 4019 4444 4944 5851 1276
Size: 102 2641 425 500 907 2816
Fragments arranged by size:
2816 2641 907 500 425 102
PciI A'CATG_T
Cuts at: 1272 5847 1272
Size: 4575 2816
PflMI CCAn_nnn'nTGG
Cuts at: 1494 1818 3697 3912 4297 1494
Size: 324 1879 215 385 4588
Fragments arranged by size:
4588 1879 385 324 215
PfoI T'CCnGG_A
Cuts at: 828 2940 828
Size: 2112 5279
PmeI GTTT'AAAC
Cuts at: 4390 4390
Size: 7391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 131 163 1214 1246 2750 2782 6530 6562 131
Size: 32 1051 32 1504 32 3748 32 960
Fragments arranged by size:
3748 1504 1051 960 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2676 2733 2676
Size: 57 7334
PstI C_TGCA'G
Cuts at: 552 1219 1877 2753 4358 4438 5123 552
Size: 667 658 876 1605 80 685 2820
Fragments arranged by size:
2820 1605 876 685 667 658 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7391
PvuII CAG'CTG
Cuts at: 553 1434 4353 4816 5176 553
Size: 881 2919 463 360 2768
Fragments arranged by size:
2919 2768 881 463 360
RsrII CG'GwC_CG
Cuts at: 5586 5586
Size: 7391
SacI G_AGCT'C
Cuts at: 1782 4405 1782
Size: 2623 4768
SalI G'TCGA_C
Cuts at: 2783 4428 2783
Size: 1645 5746
SapI GCTCTTCn'nnn_
Cuts at: 164 5414 5624 164
Size: 5250 210 1931
Fragments arranged by size:
5250 1931 210
SbfI CC_TGCA'GG
Cuts at: 4438 4438
Size: 7391
ScaI AGT'ACT
Cuts at: 6908 6908
Size: 7391
SexAI A'CCwGG_T
Cuts at: 2360 3092 2360
Size: 732 6659
SfcI C'TryA_G
Cuts at: 33 548 578 1215 1873 2749 4284 4354 4434
Size: 515 30 637 658 876 1535 70 80
Cuts at: 4434 5119 6112 6303 33
Size: 685 993 191 1121
Fragments arranged by size:
1535 1121 993 876 685 658 637 515
191 80 70 30
SfoI GGC'GCC
Cuts at: 4823 5071 4823
Size: 248 7143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7391
SmaI CCC'GGG
Cuts at: 4413 4413
Size: 7391
SmlI C'TyrA_G
Cuts at: 287 358 735 1957 3253 4546 5461 5953 6215
Size: 71 377 1222 1296 1293 915 492 262
Cuts at: 6215 287
Size: 1463
Fragments arranged by size:
1463 1296 1293 1222 915 492 377 262
71
SpeI A'CTAG_T
Cuts at: 67 679 67
Size: 612 6779
SphI G_CATG'C
Cuts at: 4444 4944 4444
Size: 500 6891
SspI AAT'ATT
Cuts at: 638 842 638
Size: 204 7187
StyI C'CwwG_G
Cuts at: 107 974 1058 1800 2319 2348 3133 4525 4879
Size: 867 84 742 519 29 785 1392 354
Cuts at: 4879 107
Size: 2619
Fragments arranged by size:
2619 1392 867 785 742 519 354 84
29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3061 3828 4974 5107 5566 5775 3061
Size: 767 1146 133 459 209 4677
Fragments arranged by size:
4677 1146 767 459 209 133
TatI w'GTAC_w
Cuts at: 1182 2070 3893 6906 1182
Size: 888 1823 3013 1667
Fragments arranged by size:
3013 1823 1667 888
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4175 4207 6908 6940 4175
Size: 32 2701 32 4626
Fragments arranged by size:
4626 2701 32 32
Tth111I GACn'n_nGTC
Cuts at: 665 5188 665
Size: 4523 2868
XbaI T'CTAG_A
Cuts at: 192 952 4422 192
Size: 760 3470 3161
Fragments arranged by size:
3470 3161 760
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 780 1163 4886 780
Size: 383 3723 3285
Fragments arranged by size:
3723 3285 383
XmaI C'CCGG_G
Cuts at: 4411 4411
Size: 7391
XmnI GAAnn'nnTTC
Cuts at: 2200 3143 3278 4172 2200
Size: 943 135 894 5419
Fragments arranged by size:
5419 943 894 135
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII ApaI AscI AvrII BcgI BfrBI
BglI BmgBI BmtI BsiWI BssHII BstZ17I ClaI CspCI
EcoNI FseI FspAI HpaI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI