Restriction map for
pF1KA0806
(Circular) (Six-base) MAPSORT of: pF1KA0806.seq Check: 485 from: 1 to: 6308
pF1KA0806 6308 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 2084 2084
Size: 6308
AccI GT'mk_AC
Cuts at: 3346 3346
Size: 6308
Acc65I G'GTAC_C
Cuts at: 2797 3324 2797
Size: 527 5781
AclI AA'CG_TT
Cuts at: 6148 6148
Size: 6308
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 4133 4565 4918 5580 1174
Size: 2959 432 353 662 1902
Fragments arranged by size:
2959 1902 662 432 353
AflII C'TTAA_G
Cuts at: 828 868 1203 828
Size: 40 335 5933
Fragments arranged by size:
5933 335 40
AflIII A'CryG_T
Cuts at: 3660 5460 5741 3660
Size: 1800 281 4227
Fragments arranged by size:
4227 1800 281
AleI CACnn'nnGTG
Cuts at: 1669 1669
Size: 6308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 249 304 249
Size: 55 6253
AlwNI CAG_nnn'CTG
Cuts at: 147 2907 3613 5051 147
Size: 2760 706 1438 1404
Fragments arranged by size:
2760 1438 1404 706
ApaLI G'TGCA_C
Cuts at: 1520 1662 5146 1520
Size: 142 3484 2682
Fragments arranged by size:
3484 2682 142
ApoI r'AATT_y
Cuts at: 624 1128 1217 1746 1818 3312 5661 5917 624
Size: 504 89 529 72 1494 2349 256 1015
Fragments arranged by size:
2349 1494 1015 529 504 256 89 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 6308
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6308
AvaI C'yCGr_G
Cuts at: 2045 2071 2231 2801 2852 3328 2045
Size: 26 160 570 51 476 5025
Fragments arranged by size:
5025 570 476 160 51 26
AvrII C'CTAG_G
Cuts at: 123 123
Size: 6308
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2607 2640 2789 2822 2607
Size: 33 149 33 6093
Fragments arranged by size:
6093 149 33 33
BamHI G'GATC_C
Cuts at: 3333 3333
Size: 6308
BanI G'GyrC_C
Cuts at: 111 117 1954 2797 3324 3738 3986 4021 111
Size: 6 1837 843 527 414 248 35 2398
Fragments arranged by size:
2398 1837 843 527 414 248 35 6
BanII G_rGCy'C
Cuts at: 2645 3322 4352 2645
Size: 677 1030 4601
Fragments arranged by size:
4601 1030 677
BbeI G_GCGC'C
Cuts at: 115 121 3742 3990 115
Size: 6 3621 248 2433
Fragments arranged by size:
3621 2433 248 6
BbsI GAAGACnn'nnnn_
Cuts at: 2076 2621 2669 2076
Size: 545 48 5715
Fragments arranged by size:
5715 545 48
BbvCI CC'TCA_GC
Cuts at: 559 2882 559
Size: 2323 3985
BciVI GTATCCnnnnn_n'
Cuts at: 1731 2958 4206 5262 6308 1731
Size: 1227 1248 1056 1046 1731
Fragments arranged by size:
1731 1248 1227 1056 1046
BclI T'GATC_A
Cuts at: 2898 3828 2898
Size: 930 5378
BfrBI ATG'CAT
Cuts at: 1024 1024
Size: 6308
BglII A'GATC_T
Cuts at: 737 832 3823 5860 737
Size: 95 2991 2037 1185
Fragments arranged by size:
2991 2037 1185 95
BlpI GC'TnA_GC
Cuts at: 3420 3420
Size: 6308
Bme1580I G_kGCm'C
Cuts at: 1524 1666 3301 3933 4026 5150 1524
Size: 142 1635 632 93 1124 2682
Fragments arranged by size:
2682 1635 1124 632 142 93
BmgBI CAC'GTC
Cuts at: 1661 1734 2064 1661
Size: 73 330 5905
Fragments arranged by size:
5905 330 73
BmrI ACTGGGnnnn_n'
Cuts at: 3520 3601 3936 6080 3520
Size: 81 335 2144 3748
Fragments arranged by size:
3748 2144 335 81
BmtI G_CTAG'C
Cuts at: 1540 1540
Size: 6308
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 247 2211 4675 247
Size: 1964 2464 1880
Fragments arranged by size:
2464 1964 1880
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 511 565 1793 2233 2285 2829 324
Size: 187 54 1228 440 52 544 3803
Fragments arranged by size:
3803 1228 544 440 187 54 52
Bpu10I CC'TnA_GC
Cuts at: 559 2882 559
Size: 2323 3985
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1363 1579 3484 4363 5077 5375 1363
Size: 216 1905 879 714 298 2296
Fragments arranged by size:
2296 1905 879 714 298 216
BsaI GGTCTCn'nnnn_
Cuts at: 235 2161 6012 235
Size: 1926 3851 531
Fragments arranged by size:
3851 1926 531
BsaAI yAC'GTr
Cuts at: 3661 3661
Size: 6308
BsaBI GATnn'nnATC
Cuts at: 6 3822 5859 6
Size: 3816 2037 455
Fragments arranged by size:
3816 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 118 128 2081 3739 3987 4689 6185 112
Size: 6 10 1953 1658 248 702 1496 235
Fragments arranged by size:
1953 1658 1496 702 248 235 10 6
BsaWI w'CCGG_w
Cuts at: 3497 4018 4696 5107 5254 5528 3497
Size: 521 678 411 147 274 4277
Fragments arranged by size:
4277 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 127 157 509 539 2827 2857 127
Size: 30 352 30 2288 30 3578
Fragments arranged by size:
3578 2288 352 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 193 2653 2899 151
Size: 42 2460 246 3560
Fragments arranged by size:
3560 2460 246 42
BseYI C'CCAG_C
Cuts at: 147 2389 2665 3143 3733 4491 5156 5564 147
Size: 2242 276 478 590 758 665 408 891
Fragments arranged by size:
2242 891 758 665 590 478 408 276
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1979 1979
Size: 6308
BsiEI CG_ry'CG
Cuts at: 104 3850 3896 5126 104
Size: 3746 46 1230 1286
Fragments arranged by size:
3746 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1524 1666 2645 3322 4100 4290 5150 1524
Size: 142 979 677 778 190 860 2682
Fragments arranged by size:
2682 979 860 778 677 190 142
BsmI GAATG_Cn'
Cuts at: 257 1002 1321 257
Size: 745 319 5244
Fragments arranged by size:
5244 745 319
BsmBI CGTCTCn'nnnn_
Cuts at: 2290 2290
Size: 6308
Bsp1286I G_dGCh'C
Cuts at: 1524 1666 2645 3301 3322 3933 4026 4100 4290
Size: 142 979 656 21 611 93 74 190
Cuts at: 4290 4352 5150 1524
Size: 62 798 2682
Fragments arranged by size:
2682 979 798 656 611 190 142 93
74 62 21
BspHI T'CATG_A
Cuts at: 4740 4740
Size: 6308
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 2410 3358 3572 3874 4255 217
Size: 2193 948 214 302 381 2270
Fragments arranged by size:
2270 2193 948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 199 3074 4600 4654 6138 6303 50
Size: 149 2875 1526 54 1484 165 55
Fragments arranged by size:
2875 1526 1484 165 149 55 54
BsrDI GCAATG_nn'
Cuts at: 185 3082 4220 185
Size: 2897 1138 2273
Fragments arranged by size:
2897 2273 1138
BsrFI r'CCGG_y
Cuts at: 114 4306 4487 114
Size: 4192 181 1935
Fragments arranged by size:
4192 1935 181
BssSI C'ACGA_G
Cuts at: 935 4579 5287 935
Size: 3644 708 1956
Fragments arranged by size:
3644 1956 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2067 2748 3685 2067
Size: 681 937 4690
Fragments arranged by size:
4690 937 681
BstBI TT'CG_AA
Cuts at: 4669 4669
Size: 6308
BstXI CCAn_nnnn'nTGG
Cuts at: 2919 2919
Size: 6308
BstYI r'GATC_y
Cuts at: 1 737 832 1563 1935 3333 3800 3823 4158
Size: 736 95 731 372 1398 467 23 335
Cuts at: 4158 4404 4710 4722 4808 5860 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1398 1052 736 731 467 449 372 335
306 246 95 86 23 12
BtgI C'CryG_G
Cuts at: 107 1958 107
Size: 1851 4457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4258 4439 4602 4258
Size: 181 163 5964
Fragments arranged by size:
5964 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1676 1686 3551 1664
Size: 12 10 1865 4421
Fragments arranged by size:
4421 1865 12 10
DraI TTT'AAA
Cuts at: 1412 3307 1412
Size: 1895 4413
DraIII CAC_nnn'GTG
Cuts at: 1703 1703
Size: 6308
DrdI GACnn_nn'nnGTC
Cuts at: 1144 1265 4014 5358 1144
Size: 121 2749 1344 2094
Fragments arranged by size:
2749 2094 1344 121
EaeI y'GGCC_r
Cuts at: 1961 2436 3893 4067 4458 4485 5830 1961
Size: 475 1457 174 391 27 1345 2439
Fragments arranged by size:
2439 1457 1345 475 391 174 27
EagI C'GGCC_G
Cuts at: 3893 3893
Size: 6308
EarI CTCTTCn'nnn_
Cuts at: 187 2484 4331 4541 187
Size: 2297 1847 210 1954
Fragments arranged by size:
2297 1954 1847 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4516 5258 5404 6118 4516
Size: 742 146 714 4706
Fragments arranged by size:
4706 742 714 146
EcoICRI GAG'CTC
Cuts at: 2643 3320 2643
Size: 677 5631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 324 511 565 1174 1793 2233 2285 2829 4133
Size: 187 54 609 619 440 52 544 1304
Cuts at: 4133 4565 4918 5580 324
Size: 432 353 662 1052
Fragments arranged by size:
1304 1052 662 619 609 544 440 432
353 187 54 52
EcoNI CCTnn'n_nnAGG
Cuts at: 3234 3234
Size: 6308
EcoO109I rG'GnC_Cy
Cuts at: 2849 3186 3447 2849
Size: 337 261 5710
Fragments arranged by size:
5710 337 261
EcoRI G'AATT_C
Cuts at: 3312 3312
Size: 6308
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4327 4327
Size: 6308
FspI TGC'GCA
Cuts at: 4089 4089
Size: 6308
HaeII r_GCGC'y
Cuts at: 115 121 313 3742 3990 5220 5991 115
Size: 6 192 3429 248 1230 771 432
Fragments arranged by size:
3429 1230 771 432 248 192 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 661 693 1897 1929 4150 4180 4182
Size: 32 82 32 1204 32 2221 30 2
Cuts at: 4182 4212 547
Size: 30 2643
Fragments arranged by size:
2643 2221 1204 82 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3347 3347
Size: 6308
KasI G'GCGC_C
Cuts at: 111 117 3738 3986 111
Size: 6 3621 248 2433
Fragments arranged by size:
3621 2433 248 6
KpnI G_GTAC'C
Cuts at: 2801 3328 2801
Size: 527 5781
MfeI C'AATT_G
Cuts at: 2469 2469
Size: 6308
MluI A'CGCG_T
Cuts at: 5741 5741
Size: 6308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5067 5251 5067
Size: 184 6124
MscI TGG'CCA
Cuts at: 1963 2438 4069 1963
Size: 475 1631 4202
Fragments arranged by size:
4202 1631 475
MslI CAynn'nnrTG
Cuts at: 1648 1669 2504 2542 3067 3179 4297 4424 1648
Size: 21 835 38 525 112 1118 127 3532
Fragments arranged by size:
3532 1118 835 525 127 112 38 21
MspA1I CmG'CkG
Cuts at: 849 1074 3090 3419 3733 4093 4877 5122 849
Size: 225 2016 329 314 360 784 245 2035
Fragments arranged by size:
2035 2016 784 360 329 314 245 225
NaeI GCC'GGC
Cuts at: 116 4489 116
Size: 4373 1935
NarI GG'CG_CC
Cuts at: 112 118 3739 3987 112
Size: 6 3621 248 2433
Fragments arranged by size:
3621 2433 248 6
NcoI C'CATG_G
Cuts at: 107 1958 107
Size: 1851 4457
NgoMIV G'CCGG_C
Cuts at: 114 4487 114
Size: 4373 1935
NheI G'CTAG_C
Cuts at: 1536 1536
Size: 6308
NsiI A_TGCA'T
Cuts at: 1026 1026
Size: 6308
NspI r_CATG'y
Cuts at: 609 2061 3361 3861 5464 609
Size: 1452 1300 500 1603 1453
Fragments arranged by size:
1603 1453 1452 1300 500
PciI A'CATG_T
Cuts at: 5460 5460
Size: 6308
PflMI CCAn_nnn'nTGG
Cuts at: 765 1703 765
Size: 938 5370
PfoI T'CCnGG_A
Cuts at: 131 266 344 2845 131
Size: 135 78 2501 3594
Fragments arranged by size:
3594 2501 135 78
PmeI GTTT'AAAC
Cuts at: 3307 3307
Size: 6308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 4754
Size: 32 6276
PpuMI rG'GwC_Cy
Cuts at: 2849 2849
Size: 6308
PshAI GACnn'nnGTC
Cuts at: 275 275
Size: 6308
PstI C_TGCA'G
Cuts at: 277 1671 1983 2346 2658 3355 4040 277
Size: 1394 312 363 312 697 685 2545
Fragments arranged by size:
2545 1394 697 685 363 312 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6308
PvuII CAG'CTG
Cuts at: 1074 3733 4093 1074
Size: 2659 360 3289
Fragments arranged by size:
3289 2659 360
RsrII CG'GwC_CG
Cuts at: 4503 4503
Size: 6308
SacI G_AGCT'C
Cuts at: 2645 3322 2645
Size: 677 5631
SalI G'TCGA_C
Cuts at: 3345 3345
Size: 6308
SapI GCTCTTCn'nnn_
Cuts at: 4331 4541 4331
Size: 210 6098
SbfI CC_TGCA'GG
Cuts at: 3355 3355
Size: 6308
ScaI AGT'ACT
Cuts at: 5825 5825
Size: 6308
SfcI C'TryA_G
Cuts at: 33 273 1667 1979 2124 2342 2619 2654 2712
Size: 240 1394 312 145 218 277 35 58
Cuts at: 2712 3351 4036 5004 5195 33
Size: 639 685 968 191 1146
Fragments arranged by size:
1394 1146 968 685 639 312 277 240
218 191 145 58 35
SfoI GGC'GCC
Cuts at: 113 119 3740 3988 113
Size: 6 3621 248 2433
Fragments arranged by size:
3621 2433 248 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6308
SgrAI Cr'CCGG_yG
Cuts at: 114 114
Size: 6308
SmaI CCC'GGG
Cuts at: 2803 3330 2803
Size: 527 5781
SmlI C'TyrA_G
Cuts at: 828 868 1203 1342 1594 2045 3463 4378 5092
Size: 40 335 139 252 451 1418 915 714
Cuts at: 5092 5354 828
Size: 262 1782
Fragments arranged by size:
1782 1418 915 714 451 335 262 252
139 40
SpeI A'CTAG_T
Cuts at: 67 1792 67
Size: 1725 4583
SphI G_CATG'C
Cuts at: 609 2061 3361 3861 609
Size: 1452 1300 500 3056
Fragments arranged by size:
3056 1452 1300 500
SspI AAT'ATT
Cuts at: 471 471
Size: 6308
StuI AGG'CCT
Cuts at: 3122 3122
Size: 6308
StyI C'CwwG_G
Cuts at: 107 123 650 813 1958 2421 2682 3442 3796
Size: 16 527 163 1145 463 261 760 354
Cuts at: 3796 107
Size: 2619
Fragments arranged by size:
2619 1145 760 527 463 354 261 163
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1704 3891 4024 4483 4692 1704
Size: 2187 133 459 209 3320
Fragments arranged by size:
3320 2187 459 209 133
TatI w'GTAC_w
Cuts at: 2949 5823 2949
Size: 2874 3434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 2418 2450 5825 5857 509
Size: 32 1877 32 3375 32 960
Fragments arranged by size:
3375 1877 960 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2907 4105 2907
Size: 1198 5110
XbaI T'CTAG_A
Cuts at: 160 1350 3339 160
Size: 1190 1989 3129
Fragments arranged by size:
3129 1989 1190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 3803 2491
Size: 1312 4996
XhoI C'TCGA_G
Cuts at: 2045 2045
Size: 6308
XmaI C'CCGG_G
Cuts at: 2801 3328 2801
Size: 527 5781
XmnI GAAnn'nnTTC
Cuts at: 1321 1421 1321
Size: 100 6208
ZraI GAC'GTC
Cuts at: 2082 2082
Size: 6308
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AloI ApaI AscI BcgI
BglI BsiWI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI NdeI
NotI NruI PacI PasI PmlI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SwaI