Restriction map for
pF1KA0815
(Circular) (Six-base) MAPSORT of: pF1KA0815.seq Check: 8345 from: 1 to: 6800
pF1KA0815 6800 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 933 1720 1900 2599 3238 3322 3496 933
Size: 787 180 699 639 84 174 4237
Fragments arranged by size:
4237 787 699 639 180 174 84
AatII G_ACGT'C
Cuts at: 1262 2777 1262
Size: 1515 5285
AccI GT'mk_AC
Cuts at: 911 3838 911
Size: 2927 3873
Acc65I G'GTAC_C
Cuts at: 3816 3816
Size: 6800
AclI AA'CG_TT
Cuts at: 6640 6640
Size: 6800
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 4625 5057 5804 6072 4625
Size: 432 747 268 5353
Fragments arranged by size:
5353 747 432 268
AfeI AGC'GCT
Cuts at: 1834 2421 2926 1834
Size: 587 505 5708
Fragments arranged by size:
5708 587 505
AflIII A'CryG_T
Cuts at: 859 2237 3182 3466 4152 5256 6233 859
Size: 1378 945 284 686 1104 977 1426
Fragments arranged by size:
1426 1378 1104 977 945 686 284
AgeI A'CCGG_T
Cuts at: 314 3287 5188 314
Size: 2973 1901 1926
Fragments arranged by size:
2973 1926 1901
AleI CACnn'nnGTG
Cuts at: 299 440 839 3181 3185 299
Size: 141 399 2342 4 3914
Fragments arranged by size:
3914 2342 399 141 4
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 574 1908 574
Size: 1334 5466
AlwNI CAG_nnn'CTG
Cuts at: 389 2228 2669 4105 5672 389
Size: 1839 441 1436 1567 1517
Fragments arranged by size:
1839 1567 1517 1436 441
ApaI G_GGCC'C
Cuts at: 851 2040 2485 2525 2743 3584 3674 3695 851
Size: 1189 445 40 218 841 90 21 3956
Fragments arranged by size:
3956 1189 841 445 218 90 40 21
ApaLI G'TGCA_C
Cuts at: 855 948 5570 855
Size: 93 4622 2085
Fragments arranged by size:
4622 2085 93
ApoI r'AATT_y
Cuts at: 3804 6153 6409 3804
Size: 2349 256 4195
Fragments arranged by size:
4195 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6800
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6800
AvaI C'yCGr_G
Cuts at: 119 484 1420 1551 2013 2113 2338 2768 3025
Size: 365 936 131 462 100 225 430 257
Cuts at: 3025 3124 3243 3706 3820 119
Size: 99 119 463 114 3099
Fragments arranged by size:
3099 936 463 462 430 365 257 225
131 119 114 100 99
BamHI G'GATC_C
Cuts at: 3825 3825
Size: 6800
BanI G'GyrC_C
Cuts at: 146 566 745 1646 2611 3226 3484 3816 4230
Size: 420 179 901 965 615 258 332 414
Cuts at: 4230 4478 4513 146
Size: 248 35 2433
Fragments arranged by size:
2433 965 901 615 420 414 332 258
248 179 35
BanII G_rGCy'C
Cuts at: 158 851 1439 1688 2020 2040 2485 2525 2743
Size: 693 588 249 332 20 445 40 218
Cuts at: 2743 2887 3079 3584 3674 3695 3733 3814 4844
Size: 144 192 505 90 21 38 81 1030
Cuts at: 4844 158
Size: 2114
Fragments arranged by size:
2114 1030 693 588 505 445 332 249
218 192 144 90 81 40 38 21
20
BbeI G_GCGC'C
Cuts at: 749 4234 4482 749
Size: 3485 248 3067
Fragments arranged by size:
3485 3067 248
BbsI GAAGACnn'nnnn_
Cuts at: 2099 2099
Size: 6800
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 891 901 925 935 891
Size: 10 24 10 6756
Fragments arranged by size:
6756 24 10 10
BciVI GTATCCnnnnn_n'
Cuts at: 4698 5459 6800 4698
Size: 761 1341 4698
Fragments arranged by size:
4698 1341 761
BclI T'GATC_A
Cuts at: 4320 4320
Size: 6800
BglI GCCn_nnn'nGGC
Cuts at: 112 239 608 1055 1397 1406 1424 1592 1901
Size: 127 369 447 342 9 18 168 309
Cuts at: 1901 2210 2249 2600 2635 2639 2729 2738 2858
Size: 309 39 351 35 4 90 9 120
Cuts at: 2858 2897 3097 3164 3497 3699 112
Size: 39 200 67 333 202 3213
Fragments arranged by size:
3213 447 369 351 342 333 309 309
202 200 168 127 120 90 67 39
39 35 18 9 9 4
BglII A'GATC_T
Cuts at: 2144 4315 6352 2144
Size: 2171 2037 2592
Fragments arranged by size:
2592 2171 2037
BlpI GC'TnA_GC
Cuts at: 3732 3912 3732
Size: 180 6620
Bme1580I G_kGCm'C
Cuts at: 187 684 851 859 952 1987 2040 2245 2303
Size: 497 167 8 93 1035 53 205 58
Cuts at: 2303 2337 2384 2485 2525 2614 2743 2922 3487
Size: 34 47 101 40 89 129 179 565
Cuts at: 3487 3584 3674 3695 3742 4425 4518 5574 187
Size: 97 90 21 47 683 93 1056 1413
Fragments arranged by size:
1413 1056 1035 683 565 497 205 179
167 129 101 97 93 93 90 89
58 53 47 47 40 34 21 8
BmgBI CAC'GTC
Cuts at: 2238 2574 2961 2238
Size: 336 387 6077
Fragments arranged by size:
6077 387 336
BmrI ACTGGGnnnn_n'
Cuts at: 669 1442 1891 2096 2387 2394 2876 2975 3125
Size: 773 449 205 291 7 482 99 150
Cuts at: 3125 4012 4093 4428 6572 669
Size: 887 81 335 2144 897
Fragments arranged by size:
2144 897 887 773 482 449 335 291
205 150 99 81 7
BmtI G_CTAG'C
Cuts at: 622 622
Size: 6800
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1059 5167 1059
Size: 4108 2692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 481 1029 1174 1207 2625 3460 3738 225
Size: 256 548 145 33 1418 835 278 3287
Fragments arranged by size:
3287 1418 835 548 278 256 145 33
Bpu10I CC'TnA_GC
Cuts at: 3537 3720 3537
Size: 183 6617
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4855 5347 5645 3976
Size: 879 492 298 5131
Fragments arranged by size:
5131 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2179 6504 2179
Size: 4325 2475
BsaAI yAC'GTr
Cuts at: 606 2478 3183 3467 3677 4153 606
Size: 1872 705 284 210 476 3253
Fragments arranged by size:
3253 1872 705 476 284 210
BsaBI GATnn'nnATC
Cuts at: 6 781 4314 6351 6
Size: 775 3533 2037 455
Fragments arranged by size:
3533 2037 775 455
BsaHI Gr'CG_yC
Cuts at: 115 746 1259 2774 3257 4231 4479 5181 6677
Size: 631 513 1515 483 974 248 702 1496
Cuts at: 6677 115
Size: 238
Fragments arranged by size:
1515 1496 974 702 631 513 483 248
238
BsaWI w'CCGG_w
Cuts at: 314 767 1461 2188 2317 3287 3989 4510 5188
Size: 453 694 727 129 970 702 521 678
Cuts at: 5188 5462 5609 6020 314
Size: 274 147 411 1094
Fragments arranged by size:
1094 970 727 702 694 678 521 453
411 274 147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1443 1473 1741 1771 3061 3091 3301 3331 1443
Size: 30 268 30 1290 30 210 30 4912
Fragments arranged by size:
4912 1290 268 210 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1171 1762 1171
Size: 591 6209
BseYI C'CCAG_C
Cuts at: 741 844 1854 2123 2130 2151 2244 2263 2310
Size: 103 1010 269 7 21 93 19 47
Cuts at: 2310 2853 2921 3583 4225 4983 5560 6056 741
Size: 543 68 662 642 758 577 496 1485
Fragments arranged by size:
1485 1010 758 662 642 577 543 496
269 103 93 68 47 21 19 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 941 1056 1108 1783 2571 2661 3159 3573 3580
Size: 115 52 675 788 90 498 414 7
Cuts at: 3580 941
Size: 4161
Fragments arranged by size:
4161 788 675 498 414 115 90 52
7
BsiEI CG_ry'CG
Cuts at: 104 1695 2600 2639 4342 4388 5596 104
Size: 1591 905 39 1703 46 1208 1308
Fragments arranged by size:
1703 1591 1308 1208 905 46 39
BsiHKAI G_wGCw'C
Cuts at: 859 952 1067 1102 1223 2020 2284 3311 3448
Size: 93 115 35 121 797 264 1027 137
Cuts at: 3448 3530 3814 4592 4782 5574 859
Size: 82 284 778 190 792 2085
Fragments arranged by size:
2085 1027 797 792 778 284 264 190
137 121 115 93 82 35
BsmI GAATG_Cn'
Cuts at: 289 289
Size: 6800
Bsp1286I G_dGCh'C
Cuts at: 158 187 684 851 859 952 1067 1102 1223
Size: 29 497 167 8 93 115 35 121
Cuts at: 1223 1439 1688 1987 2020 2040 2245 2284 2303
Size: 216 249 299 33 20 205 39 19
Cuts at: 2303 2337 2384 2485 2525 2614 2743 2887 2922
Size: 34 47 101 40 89 129 144 35
Cuts at: 2922 3079 3311 3448 3487 3530 3584 3674 3695
Size: 157 232 137 39 43 54 90 21
Cuts at: 3695 3733 3742 3814 4425 4518 4592 4782 4844
Size: 38 9 72 611 93 74 190 62
Cuts at: 4844 5574 158
Size: 730 1384
Fragments arranged by size:
1384 730 611 497 299 249 232 216
205 190 167 157 144 137 129 121
115 101 93 93 90 89 74 72
62 54 47 43 40 39 39 38
35 35 34 33 29 21 20 19
9 8
BspEI T'CCGG_A
Cuts at: 1461 6020 1461
Size: 4559 2241
BspHI T'CATG_A
Cuts at: 5976 5976
Size: 6800
BspMI ACCTGCnnnn'nnnn_
Cuts at: 397 907 933 1396 1525 1720 1900 2599 3238
Size: 510 26 463 129 195 180 699 639
Cuts at: 3238 3322 3496 3850 4064 4366 4747 397
Size: 84 174 354 214 302 381 2450
Fragments arranged by size:
2450 699 639 510 463 381 354 302
214 195 180 174 129 84 26
BsrBI CCG'CTC
Cuts at: 50 1925 3776 5092 5146 6630 6795 50
Size: 1875 1851 1316 54 1484 165 55
Fragments arranged by size:
1875 1851 1484 1316 165 55 54
BsrDI GCAATG_nn'
Cuts at: 426 1211 1452 4712 426
Size: 785 241 3260 2514
Fragments arranged by size:
3260 2514 785 241
BsrFI r'CCGG_y
Cuts at: 238 256 314 479 755 988 995 1084 1111
Size: 18 58 165 276 233 7 89 27
Cuts at: 1111 1118 1643 2066 2347 2599 2638 2789 2857
Size: 7 525 423 281 252 39 151 68
Cuts at: 2857 3223 3253 3287 3530 4798 4979 5188 238
Size: 366 30 34 243 1268 181 209 1850
Fragments arranged by size:
1850 1268 525 423 366 281 276 252
243 233 209 181 165 151 89 68
58 39 34 30 27 18 7 7
BssHII G'CGCG_C
Cuts at: 172 172
Size: 6800
BssSI C'ACGA_G
Cuts at: 1126 5071 5429 1126
Size: 3945 358 2497
Fragments arranged by size:
3945 2497 358
BstAPI GCAn_nnn'nTGC
Cuts at: 389 1622 2228 2618 2669 4177 389
Size: 1233 606 390 51 1508 3012
Fragments arranged by size:
3012 1508 1233 606 390 51
BstBI TT'CG_AA
Cuts at: 5161 5161
Size: 6800
BstEII G'GTnAC_C
Cuts at: 259 259
Size: 6800
BstXI CCAn_nnnn'nTGG
Cuts at: 301 2252 2861 2965 3187 301
Size: 1951 609 104 222 3914
Fragments arranged by size:
3914 1951 609 222 104
BstYI r'GATC_y
Cuts at: 1 2144 3825 4292 4315 4650 4896 5908 5994
Size: 2143 1681 467 23 335 246 1012 86
Cuts at: 5994 6006 6352 1
Size: 12 346 449
Fragments arranged by size:
2143 1681 1012 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 126 684 822 867 1050 1720 2046 2427
Size: 19 558 138 45 183 670 326 381
Cuts at: 2427 2485 3092 3479 107
Size: 58 607 387 3428
Fragments arranged by size:
3428 670 607 558 387 381 326 183
138 58 45 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 451 4750 4931 5094 451
Size: 4299 181 163 2157
Fragments arranged by size:
4299 2157 181 163
BtsI GCAGTG_nn'
Cuts at: 766 1068 2068 2301 2322 2506 2670 3390 3649
Size: 302 1000 233 21 184 164 720 259
Cuts at: 3649 3742 4043 766
Size: 93 301 3523
Fragments arranged by size:
3523 1000 720 302 301 259 233 184
164 93 21
ClaI AT'CG_AT
Cuts at: 905 905
Size: 6800
DraI TTT'AAA
Cuts at: 3799 3799
Size: 6800
DraIII CAC_nnn'GTG
Cuts at: 3654 3654
Size: 6800
DrdI GACnn_nn'nnGTC
Cuts at: 4506 5364 4506
Size: 858 5942
EaeI y'GGCC_r
Cuts at: 134 395 753 1082 1341 1692 1974 2424 2597
Size: 261 358 329 259 351 282 450 173
Cuts at: 2597 2636 2730 3089 3513 4385 4559 4950 4977
Size: 39 94 359 424 872 174 391 27
Cuts at: 4977 6322 134
Size: 1345 612
Fragments arranged by size:
1345 872 612 450 424 391 359 358
351 329 282 261 259 174 173 94
39 27
EagI C'GGCC_G
Cuts at: 1692 2597 2636 4385 1692
Size: 905 39 1749 4107
Fragments arranged by size:
4107 1749 905 39
EarI CTCTTCn'nnn_
Cuts at: 1249 1271 4823 5033 1249
Size: 22 3552 210 3016
Fragments arranged by size:
3552 3016 210 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3011 5008 5318 5464 6610 3011
Size: 1997 310 146 1146 3201
Fragments arranged by size:
3201 1997 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2018 3812 2018
Size: 1794 5006
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 225 481 1029 1174 1207 2625 3460 3738 4625
Size: 256 548 145 33 1418 835 278 887
Cuts at: 4625 5057 5804 6072 225
Size: 432 747 268 953
Fragments arranged by size:
1418 953 887 835 747 548 432 278
268 256 145 33
EcoNI CCTnn'n_nnAGG
Cuts at: 232 232
Size: 6800
EcoO109I rG'GnC_Cy
Cuts at: 165 386 582 707 848 896 1385 1514 1535
Size: 221 196 125 141 48 489 129 21
Cuts at: 1535 1664 2036 2037 2389 2582 2739 2740 2817
Size: 129 372 1 352 193 157 1 77
Cuts at: 2817 2969 3098 3333 3670 3692 3939 165
Size: 152 129 235 337 22 247 3026
Fragments arranged by size:
3026 489 372 352 337 247 235 221
196 193 157 152 141 129 129 129
125 77 48 22 21 1 1
EcoRI G'AATT_C
Cuts at: 3804 3804
Size: 6800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 378 1836 2300 4819 378
Size: 1458 464 2519 2359
Fragments arranged by size:
2519 2359 1458 464
FspI TGC'GCA
Cuts at: 4581 4581
Size: 6800
HaeII r_GCGC'y
Cuts at: 257 749 1836 2423 2928 3420 3537 4234 4482
Size: 492 1087 587 505 492 117 697 248
Cuts at: 4482 5504 6483 257
Size: 1022 979 574
Fragments arranged by size:
1087 1022 979 697 587 574 505 492
492 248 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1443 1475 3242 3274 3700 3732 4642 4672 4674
Size: 32 1767 32 426 32 910 30 2
Cuts at: 4674 4704 1443
Size: 30 3539
Fragments arranged by size:
3539 1767 910 426 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 912 3839 912
Size: 2927 3873
KasI G'GCGC_C
Cuts at: 745 4230 4478 745
Size: 3485 248 3067
Fragments arranged by size:
3485 3067 248
KpnI G_GTAC'C
Cuts at: 3820 3820
Size: 6800
MluI A'CGCG_T
Cuts at: 6233 6233
Size: 6800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 5471 5655 5471
Size: 184 6616
MscI TGG'CCA
Cuts at: 397 1343 1976 2426 3515 4561 397
Size: 946 633 450 1089 1046 2636
Fragments arranged by size:
2636 1089 1046 946 633 450
MslI CAynn'nnrTG
Cuts at: 299 440 839 1216 3181 3185 4789 4916 299
Size: 141 399 377 1965 4 1604 127 2183
Fragments arranged by size:
2183 1965 1604 399 377 141 127 4
MspA1I CmG'CkG
Cuts at: 128 554 593 625 794 869 1499 1544 1802
Size: 426 39 32 169 75 630 45 258
Cuts at: 1802 2123 2174 2228 2267 2330 2450 2487 2538
Size: 321 51 54 39 63 120 37 51
Cuts at: 2538 2579 2728 2747 2798 2843 2933 2947 2978
Size: 41 149 19 51 45 90 14 31
Cuts at: 2978 3054 3107 3263 3359 3494 3587 3736 3911
Size: 76 53 156 96 135 93 149 175
Cuts at: 3911 4225 4585 5598 5843 128
Size: 314 360 1013 245 1085
Fragments arranged by size:
1085 1013 630 426 360 321 314 258
245 175 169 156 149 149 135 120
96 93 90 76 75 63 54 53
51 51 51 45 45 41 39 39
37 32 31 19 14
NaeI GCC'GGC
Cuts at: 240 481 757 990 1086 1113 2601 2640 2791
Size: 241 276 233 96 27 1488 39 151
Cuts at: 2791 2859 3255 4981 240
Size: 68 396 1726 2059
Fragments arranged by size:
2059 1726 1488 396 276 241 233 151
96 68 39 27
NarI GG'CG_CC
Cuts at: 746 4231 4479 746
Size: 3485 248 3067
Fragments arranged by size:
3485 3067 248
NcoI C'CATG_G
Cuts at: 107 684 822 107
Size: 577 138 6085
Fragments arranged by size:
6085 577 138
NgoMIV G'CCGG_C
Cuts at: 238 479 755 988 1084 1111 2599 2638 2789
Size: 241 276 233 96 27 1488 39 151
Cuts at: 2789 2857 3253 4979 238
Size: 68 396 1726 2059
Fragments arranged by size:
2059 1726 1488 396 276 241 233 151
96 68 39 27
NheI G'CTAG_C
Cuts at: 618 618
Size: 6800
NspI r_CATG'y
Cuts at: 561 863 1889 2159 2629 3853 4353 5260 561
Size: 302 1026 270 470 1224 500 907 2101
Fragments arranged by size:
2101 1224 1026 907 500 470 302 270
PasI CC'CwG_GG
Cuts at: 414 702 1681 2508 3412 3726 414
Size: 288 979 827 904 314 3488
Fragments arranged by size:
3488 979 904 827 314 288
PciI A'CATG_T
Cuts at: 859 5256 859
Size: 4397 2403
PfoI T'CCnGG_A
Cuts at: 497 1223 1247 3769 497
Size: 726 24 2522 3528
Fragments arranged by size:
3528 2522 726 24
PmeI GTTT'AAAC
Cuts at: 3799 3799
Size: 6800
PmlI CAC'GTG
Cuts at: 606 3183 3467 3677 606
Size: 2577 284 210 3729
Fragments arranged by size:
3729 2577 284 210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1289 1321 5939 5971 1289
Size: 32 4618 32 2118
Fragments arranged by size:
4618 2118 32 32
PpuMI rG'GwC_Cy
Cuts at: 386 707 896 1385 1514 2389 386
Size: 321 189 489 129 875 4797
Fragments arranged by size:
4797 875 489 321 189 129
PshAI GACnn'nnGTC
Cuts at: 2876 2876
Size: 6800
PspOMI G'GGCC_C
Cuts at: 847 2036 2481 2521 2739 3580 3670 3691 847
Size: 1189 445 40 218 841 90 21 3956
Fragments arranged by size:
3956 1189 841 445 218 90 40 21
PspXI vC'TCGA_Gb
Cuts at: 2013 2013
Size: 6800
PstI C_TGCA'G
Cuts at: 832 1147 1354 1375 1486 1498 2329 2401 2419
Size: 315 207 21 111 12 831 72 18
Cuts at: 2419 2932 3325 3847 4532 832
Size: 513 393 522 685 3100
Fragments arranged by size:
3100 831 685 522 513 393 315 207
111 72 21 18 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6800
PvuII CAG'CTG
Cuts at: 554 794 1499 1802 2267 2330 2538 2933 3054
Size: 240 705 303 465 63 208 395 121
Cuts at: 3054 3359 3587 4225 4585 554
Size: 305 228 638 360 2769
Fragments arranged by size:
2769 705 638 465 395 360 305 303
240 228 208 121 63
RsrII CG'GwC_CG
Cuts at: 4995 4995
Size: 6800
SacI G_AGCT'C
Cuts at: 2020 3814 2020
Size: 1794 5006
SacII CC_GC'GG
Cuts at: 129 870 2488 129
Size: 741 1618 4441
Fragments arranged by size:
4441 1618 741
SalI G'TCGA_C
Cuts at: 910 3837 910
Size: 2927 3873
SapI GCTCTTCn'nnn_
Cuts at: 4823 5033 4823
Size: 210 6590
SbfI CC_TGCA'GG
Cuts at: 3847 3847
Size: 6800
ScaI AGT'ACT
Cuts at: 6317 6317
Size: 6800
SfcI C'TryA_G
Cuts at: 33 828 1143 1350 1371 1482 1494 2325 2397
Size: 795 315 207 21 111 12 831 72
Cuts at: 2397 2415 2928 3321 3843 4528 5521 5712 33
Size: 18 513 393 522 685 993 191 1121
Fragments arranged by size:
1121 993 831 795 685 522 513 393
315 207 191 111 72 21 18 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2738 3097 3164 2738
Size: 359 67 6374
Fragments arranged by size:
6374 359 67
SfoI GGC'GCC
Cuts at: 747 4232 4480 747
Size: 3485 248 3067
Fragments arranged by size:
3485 3067 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6800
SgrAI Cr'CCGG_yG
Cuts at: 256 314 3530 256
Size: 58 3216 3526
Fragments arranged by size:
3526 3216 58
SmaI CCC'GGG
Cuts at: 121 486 1553 2340 2770 3027 3245 3822 121
Size: 365 1067 787 430 257 218 577 3099
Fragments arranged by size:
3099 1067 787 577 430 365 257 218
SmlI C'TyrA_G
Cuts at: 2013 3955 4870 5362 5624 2013
Size: 1942 915 492 262 3189
Fragments arranged by size:
3189 1942 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6800
SphI G_CATG'C
Cuts at: 561 1889 2159 2629 3853 4353 561
Size: 1328 270 470 1224 500 3008
Fragments arranged by size:
3008 1328 1224 500 470 270
SrfI GCCC'GGGC
Cuts at: 3027 3027
Size: 6800
StuI AGG'CCT
Cuts at: 637 2914 3520 637
Size: 2277 606 3917
Fragments arranged by size:
3917 2277 606
StyI C'CwwG_G
Cuts at: 107 150 684 822 1589 3934 4288 107
Size: 43 534 138 767 2345 354 2619
Fragments arranged by size:
2619 2345 767 534 354 138 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 715 2324 3050 3179 3193 4383 4516 4975 5184
Size: 1609 726 129 14 1190 133 459 209
Cuts at: 5184 715
Size: 2331
Fragments arranged by size:
2331 1609 1190 726 459 209 133 129
14
TatI w'GTAC_w
Cuts at: 6315 6315
Size: 6800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1741 1762 1773 1794 3301 3333 6317 6349 1741
Size: 21 11 21 1507 32 2984 32 2192
Fragments arranged by size:
2984 2192 1507 32 32 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 1258 1525 2572 3631 4597 1258
Size: 267 1047 1059 966 3461
Fragments arranged by size:
3461 1059 1047 966 267
XbaI T'CTAG_A
Cuts at: 3831 3831
Size: 6800
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 682 841 2515 2551 2962 3289 3552 4295 682
Size: 159 1674 36 411 327 263 743 3187
Fragments arranged by size:
3187 1674 743 411 327 263 159 36
XhoI C'TCGA_G
Cuts at: 2013 2013
Size: 6800
XmaI C'CCGG_G
Cuts at: 119 484 1551 2338 2768 3025 3243 3820 119
Size: 365 1067 787 430 257 218 577 3099
Fragments arranged by size:
3099 1067 787 577 430 365 257 218
ZraI GAC'GTC
Cuts at: 1260 2775 1260
Size: 1515 5285
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BsrGI BstZ17I Bsu36I CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NotI NruI NsiI
PacI PflMI PsiI PsrI SanDI SexAI SnaBI SspI
SwaI XmnI