Restriction map for
pF1KA0819
(Circular) (Six-base) MAPSORT of: pF1KA0819.seq Check: 5549 from: 1 to: 8867
pF1KA0819 8867 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 187 5424 187
Size: 5237 3630
AatII G_ACGT'C
Cuts at: 2900 2900
Size: 8867
AccI GT'mk_AC
Cuts at: 2420 5640 5905 2420
Size: 3220 265 5382
Fragments arranged by size:
5382 3220 265
Acc65I G'GTAC_C
Cuts at: 2832 5883 2832
Size: 3051 5816
AclI AA'CG_TT
Cuts at: 8707 8707
Size: 8867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1470 3912 4864 5829 6692 7124 7871 8139 1470
Size: 2442 952 965 863 432 747 268 2198
Fragments arranged by size:
2442 2198 965 952 863 747 432 268
AflII C'TTAA_G
Cuts at: 5389 5686 5389
Size: 297 8570
AflIII A'CryG_T
Cuts at: 6219 7323 8300 6219
Size: 1104 977 6786
Fragments arranged by size:
6786 1104 977
AgeI A'CCGG_T
Cuts at: 158 7255 158
Size: 7097 1770
AhdI GACnn_n'nnGTC
Cuts at: 3183 5127 3183
Size: 1944 6923
AleI CACnn'nnGTG
Cuts at: 3880 3880
Size: 8867
AlwNI CAG_nnn'CTG
Cuts at: 939 1158 2295 2379 4680 6172 7739 939
Size: 219 1137 84 2301 1492 1567 2067
Fragments arranged by size:
2301 2067 1567 1492 1137 219 84
ApaI G_GGCC'C
Cuts at: 3392 3902 4900 3392
Size: 510 998 7359
Fragments arranged by size:
7359 998 510
ApaLI G'TGCA_C
Cuts at: 357 718 3511 7637 357
Size: 361 2793 4126 1587
Fragments arranged by size:
4126 2793 1587 361
ApoI r'AATT_y
Cuts at: 652 751 814 849 925 1620 2302 2539 4480
Size: 99 63 35 76 695 682 237 1941
Cuts at: 4480 5871 8220 8476 652
Size: 1391 2349 256 1043
Fragments arranged by size:
2349 1941 1391 1043 695 682 256 237
99 76 63 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 8867
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8867
AvaI C'yCGr_G
Cuts at: 1085 1343 2186 3868 3901 4248 4349 4513 5609
Size: 258 843 1682 33 347 101 164 1096
Cuts at: 5609 5887 1085
Size: 278 4065
Fragments arranged by size:
4065 1682 1096 843 347 278 258 164
101 33
AvrII C'CTAG_G
Cuts at: 624 624
Size: 8867
BamHI G'GATC_C
Cuts at: 3011 5892 3011
Size: 2881 5986
BanI G'GyrC_C
Cuts at: 529 1688 2650 2764 2832 2884 4738 5219 5883
Size: 1159 962 114 68 52 1854 481 664
Cuts at: 5883 6297 6545 6580 529
Size: 414 248 35 2816
Fragments arranged by size:
2816 1854 1159 962 664 481 414 248
114 68 52 35
BanII G_rGCy'C
Cuts at: 212 1076 1408 2353 2741 3392 3580 3875 3902
Size: 864 332 945 388 651 188 295 27
Cuts at: 3902 3908 4156 4262 4298 4828 4838 4900 4930
Size: 6 248 106 36 530 10 62 30
Cuts at: 4930 5409 5657 5881 6911 212
Size: 479 248 224 1030 2168
Fragments arranged by size:
2168 1030 945 864 651 530 479 388
332 295 248 248 224 188 106 62
36 30 27 10 6
BbeI G_GCGC'C
Cuts at: 2654 2888 4742 5223 6301 6549 2654
Size: 234 1854 481 1078 248 4972
Fragments arranged by size:
4972 1854 1078 481 248 234
BbsI GAAGACnn'nnnn_
Cuts at: 4174 5076 5633 4174
Size: 902 557 7408
Fragments arranged by size:
7408 902 557
BbvCI CC'TCA_GC
Cuts at: 2816 3224 3247 4764 2816
Size: 408 23 1517 6919
Fragments arranged by size:
6919 1517 408 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2274 2308 4282 4316 2274
Size: 34 1974 34 6825
Fragments arranged by size:
6825 1974 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 592 1529 6765 7526 8867 592
Size: 937 5236 761 1341 592
Fragments arranged by size:
5236 1341 937 761 592
BclI T'GATC_A
Cuts at: 761 6387 761
Size: 5626 3241
BglI GCCn_nnn'nGGC
Cuts at: 304 844 1129 1320 4904 304
Size: 540 285 191 3584 4267
Fragments arranged by size:
4267 3584 540 285 191
BglII A'GATC_T
Cuts at: 1731 6382 8419 1731
Size: 4651 2037 2179
Fragments arranged by size:
4651 2179 2037
BlpI GC'TnA_GC
Cuts at: 1114 4361 4705 5227 5979 1114
Size: 3247 344 522 752 4002
Fragments arranged by size:
4002 3247 752 522 344
Bme1580I G_kGCm'C
Cuts at: 361 714 722 2767 3220 3392 3515 3902 4900
Size: 353 8 2045 453 172 123 387 998
Cuts at: 4900 6492 6585 7641 361
Size: 1592 93 1056 1587
Fragments arranged by size:
2045 1592 1587 1056 998 453 387 353
172 123 93 8
BmgBI CAC'GTC
Cuts at: 642 642
Size: 8867
BmrI ACTGGGnnnn_n'
Cuts at: 439 3678 3787 4183 6079 6160 6495 8639 439
Size: 3239 109 396 1896 81 335 2144 667
Fragments arranged by size:
3239 2144 1896 667 396 335 109 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4749 4890 5295 7234 4749
Size: 141 405 1939 6382
Fragments arranged by size:
6382 1939 405 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 2752 2806 3262 4209 4588 4914 5133 5238
Size: 2171 54 456 947 379 326 219 105
Cuts at: 5238 5479 5638 5758 5797 5839 581
Size: 241 159 120 39 42 3609
Fragments arranged by size:
3609 2171 947 456 379 326 241 219
159 120 105 54 42 39
Bpu10I CC'TnA_GC
Cuts at: 2068 2816 2863 3224 3247 3452 4282 4764 2068
Size: 748 47 361 23 205 830 482 6171
Fragments arranged by size:
6171 830 748 482 361 205 47 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 250 3979 4449 5290 6043 6922 7414 7712
Size: 72 3729 470 841 753 879 492 298
Cuts at: 7712 178
Size: 1333
Fragments arranged by size:
3729 1333 879 841 753 492 470 298
72
BsaI GGTCTCn'nnnn_
Cuts at: 121 406 1397 2589 3338 8571 121
Size: 285 991 1192 749 5233 417
Fragments arranged by size:
5233 1192 991 749 417 285
BsaAI yAC'GTr
Cuts at: 2424 6220 2424
Size: 3796 5071
BsaBI GATnn'nnATC
Cuts at: 6 6381 8418 6
Size: 6375 2037 455
Fragments arranged by size:
6375 2037 455
BsaHI Gr'CG_yC
Cuts at: 2651 2885 2897 4739 4759 5220 6298 6546 7248
Size: 234 12 1842 20 461 1078 248 702
Cuts at: 7248 8744 2651
Size: 1496 2774
Fragments arranged by size:
2774 1842 1496 1078 702 461 248 234
20 12
BsaWI w'CCGG_w
Cuts at: 158 2462 2835 3329 6056 6577 7255 7529 7676
Size: 2304 373 494 2727 521 678 274 147
Cuts at: 7676 8087 158
Size: 411 938
Fragments arranged by size:
2727 2304 938 678 521 494 411 373
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 232 262 3136 3166 4138 4168 4978 5008 5689
Size: 30 2874 30 972 30 810 30 681
Cuts at: 5689 5719 232
Size: 30 3380
Fragments arranged by size:
3380 2874 972 810 681 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 127 2035 2163 2174 2185 2188 2213 2749 2854
Size: 1908 128 11 11 3 25 536 105
Cuts at: 2854 2905 2944 2947 2956 2959 2962 2965 2968
Size: 51 39 3 9 3 3 3 3
Cuts at: 2968 3058 3100 3103 3106 3109 3112 3130 3133
Size: 90 42 3 3 3 3 18 3
Cuts at: 3133 3136 3139 3154 3157 3280 3451 3844 4120
Size: 3 3 15 3 123 171 393 276
Cuts at: 4120 4146 4270 4390 4423 4651 4846 4879 5332
Size: 26 124 120 33 228 195 33 453
Cuts at: 5332 5398 5455 5710 5713 5794 5818 127
Size: 66 57 255 3 81 24 3176
Fragments arranged by size:
3176 1908 536 453 393 276 255 228
195 171 128 124 123 120 105 90
81 66 57 51 42 39 33 33
26 25 24 18 15 11 11 9
3 3 3 3 3 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 138 1148 1680 2140 2288 2740 3219 3619 3641
Size: 1010 532 460 148 452 479 400 22
Cuts at: 3641 3647 3907 4020 5026 5137 6292 7050 7627
Size: 6 260 113 1006 111 1155 758 577
Cuts at: 7627 8123 138
Size: 496 882
Fragments arranged by size:
1155 1010 1006 882 758 577 532 496
479 460 452 400 260 148 113 111
22 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 5578 4129
Size: 1449 7418
BsiEI CG_ry'CG
Cuts at: 104 6409 6455 7663 104
Size: 6305 46 1208 1308
Fragments arranged by size:
6305 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 361 722 1076 2224 2858 3515 4156 5657
Size: 149 361 354 1148 634 657 641 1501
Cuts at: 5657 5881 6659 6849 7641 212
Size: 224 778 190 792 1438
Fragments arranged by size:
1501 1438 1148 792 778 657 641 634
361 354 224 190 149
BsmI GAATG_Cn'
Cuts at: 2319 5334 2319
Size: 3015 5852
BsmBI CGTCTCn'nnnn_
Cuts at: 1169 1169
Size: 8867
Bsp1286I G_dGCh'C
Cuts at: 212 361 714 722 1076 1408 2224 2353 2741
Size: 149 353 8 354 332 816 129 388
Cuts at: 2741 2767 2858 3220 3392 3515 3580 3875 3902
Size: 26 91 362 172 123 65 295 27
Cuts at: 3902 3908 4156 4262 4298 4828 4838 4900 4930
Size: 6 248 106 36 530 10 62 30
Cuts at: 4930 5409 5657 5881 6492 6585 6659 6849 6911
Size: 479 248 224 611 93 74 190 62
Cuts at: 6911 7641 212
Size: 730 1438
Fragments arranged by size:
1438 816 730 611 530 479 388 362
354 353 332 295 248 248 224 190
172 149 129 123 106 93 91 74
65 62 62 36 30 27 26 10
8 6
BspEI T'CCGG_A
Cuts at: 8087 8087
Size: 8867
BspHI T'CATG_A
Cuts at: 1892 8043 1892
Size: 6151 2716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 187 5424 5917 6131 6433 6814 187
Size: 5237 493 214 302 381 2240
Fragments arranged by size:
5237 2240 493 381 302 214
BsrBI CCG'CTC
Cuts at: 50 248 2775 7159 7213 8697 8862 50
Size: 198 2527 4384 54 1484 165 55
Fragments arranged by size:
4384 2527 1484 198 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1656 1859 6779 1656
Size: 203 4920 3744
Fragments arranged by size:
4920 3744 203
BsrFI r'CCGG_y
Cuts at: 158 2598 4790 5009 5630 6865 7046 7255 158
Size: 2440 2192 219 621 1235 181 209 1770
Fragments arranged by size:
2440 2192 1770 1235 621 219 209 181
BssSI C'ACGA_G
Cuts at: 7138 7496 7138
Size: 358 8509
BstAPI GCAn_nnn'nTGC
Cuts at: 1029 1158 5184 6244 1029
Size: 129 4026 1060 3652
Fragments arranged by size:
4026 3652 1060 129
BstBI TT'CG_AA
Cuts at: 7228 7228
Size: 8867
BstXI CCAn_nnnn'nTGG
Cuts at: 2685 3732 4020 4629 5040 2685
Size: 1047 288 609 411 6512
Fragments arranged by size:
6512 1047 609 411 288
BstYI r'GATC_y
Cuts at: 1 1731 2050 3011 3498 3854 5892 6359 6382
Size: 1730 319 961 487 356 2038 467 23
Cuts at: 6382 6717 6963 7975 8061 8073 8419 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
2038 1730 1012 961 487 467 449 356
346 335 319 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 677 2806 3434 4734 677
Size: 2129 628 1300 4810
Fragments arranged by size:
4810 2129 1300 628
BtgI C'CryG_G
Cuts at: 107 819 1916 1940 2684 2992 3596 4613 4691
Size: 712 1097 24 744 308 604 1017 78
Cuts at: 4691 5159 5573 107
Size: 468 414 3401
Fragments arranged by size:
3401 1097 1017 744 712 604 468 414
308 78 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 819 2730 6817 6998 7161 819
Size: 1911 4087 181 163 2525
Fragments arranged by size:
4087 2525 1911 181 163
BtsI GCAGTG_nn'
Cuts at: 2609 2821 5678 6110 2609
Size: 212 2857 432 5366
Fragments arranged by size:
5366 2857 432 212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 939 974 939
Size: 35 8832
DraI TTT'AAA
Cuts at: 5866 5866
Size: 8867
DraIII CAC_nnn'GTG
Cuts at: 371 5184 371
Size: 4813 4054
DrdI GACnn_nn'nnGTC
Cuts at: 163 6573 7431 163
Size: 6410 858 1599
Fragments arranged by size:
6410 1599 858
EaeI y'GGCC_r
Cuts at: 496 830 1205 1321 1546 2079 2297 2687 3589
Size: 334 375 116 225 533 218 390 902
Cuts at: 3589 3713 4504 4538 4631 4694 6452 6626 7017
Size: 124 791 34 93 63 1758 174 391
Cuts at: 7017 7044 8389 496
Size: 27 1345 974
Fragments arranged by size:
1758 1345 974 902 791 533 391 390
375 334 225 218 174 124 116 93
63 34 27
EagI C'GGCC_G
Cuts at: 6452 6452
Size: 8867
EarI CTCTTCn'nnn_
Cuts at: 884 2140 2303 2587 3035 3077 3107 3143 3356
Size: 1256 163 284 448 42 30 36 213
Cuts at: 3356 3838 4585 4663 4933 4942 4966 5705 6890
Size: 482 747 78 270 9 24 739 1185
Cuts at: 6890 7100 884
Size: 210 2651
Fragments arranged by size:
2651 1256 1185 747 739 482 448 284
270 213 210 163 78 42 36 30
24 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3847 4194 7075 7385 7531 8677 3847
Size: 347 2881 310 146 1146 4037
Fragments arranged by size:
4037 2881 1146 347 310 146
EcoICRI GAG'CTC
Cuts at: 210 1074 4154 5655 5879 210
Size: 864 3080 1501 224 3198
Fragments arranged by size:
3198 3080 1501 864 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 1470 2752 2806 3262 3912 4209 4588 4864
Size: 889 1282 54 456 650 297 379 276
Cuts at: 4864 4914 5133 5238 5479 5638 5758 5797 5829
Size: 50 219 105 241 159 120 39 32
Cuts at: 5829 5839 6692 7124 7871 8139 581
Size: 10 853 432 747 268 1309
Fragments arranged by size:
1309 1282 889 853 747 650 456 432
379 297 276 268 241 219 159 120
105 54 50 39 32 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2809 3826 4272 4540 2809
Size: 1017 446 268 7136
Fragments arranged by size:
7136 1017 446 268
EcoO109I rG'GnC_Cy
Cuts at: 2635 3304 3388 3389 3804 3898 4748 4869 4896
Size: 669 84 1 415 94 850 121 27
Cuts at: 4896 5814 6006 2635
Size: 918 192 5496
Fragments arranged by size:
5496 918 850 669 415 192 121 94
84 27 1
EcoRI G'AATT_C
Cuts at: 652 814 1620 5871 652
Size: 162 806 4251 3648
Fragments arranged by size:
4251 3648 806 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5100 5701 6886 5100
Size: 601 1185 7081
Fragments arranged by size:
7081 1185 601
FseI GG_CCGG'CC
Cuts at: 5013 5013
Size: 8867
FspI TGC'GCA
Cuts at: 6648 6648
Size: 8867
HaeII r_GCGC'y
Cuts at: 2654 2888 3444 4100 4681 4742 4791 5223 5491
Size: 234 556 656 581 61 49 432 268
Cuts at: 5491 5773 6301 6549 7571 8550 2654
Size: 282 528 248 1022 979 2971
Fragments arranged by size:
2971 1022 979 656 581 556 528 432
282 268 248 234 61 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 232 264 454 486 1921 1953 2042 2074 2076
Size: 32 190 32 1435 32 89 32 2
Cuts at: 2076 2108 3136 3168 3169 3201 3478 3510 4036
Size: 32 1028 32 1 32 277 32 526
Cuts at: 4036 4068 4978 5010 5113 5145 5396 5428 5689
Size: 32 910 32 103 32 251 32 261
Cuts at: 5689 5721 6709 6739 6741 6771 232
Size: 32 988 30 2 30 2328
Fragments arranged by size:
2328 1435 1028 988 910 526 277 261
251 190 103 89 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2 2 1
HincII GTy'rAC
Cuts at: 1536 5641 5906 1536
Size: 4105 265 4497
Fragments arranged by size:
4497 4105 265
HindIII A'AGCT_T
Cuts at: 274 274
Size: 8867
KasI G'GCGC_C
Cuts at: 2650 2884 4738 5219 6297 6545 2650
Size: 234 1854 481 1078 248 4972
Fragments arranged by size:
4972 1854 1078 481 248 234
KpnI G_GTAC'C
Cuts at: 2836 5887 2836
Size: 3051 5816
MluI A'CGCG_T
Cuts at: 8300 8300
Size: 8867
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 760 787 3167 3236 4103 4184 4865 5057
Size: 142 27 2380 69 867 81 681 192
Cuts at: 5057 5570 7538 7722 618
Size: 513 1968 184 1763
Fragments arranged by size:
2380 1968 1763 867 681 513 192 184
142 81 69 27
MscI TGG'CCA
Cuts at: 498 832 1207 1323 2081 2299 2689 4540 4696
Size: 334 375 116 758 218 390 1851 156
Cuts at: 4696 6628 498
Size: 1932 2737
Fragments arranged by size:
2737 1932 1851 758 390 375 334 218
156 116
MslI CAynn'nnrTG
Cuts at: 644 1225 1568 1945 3880 4336 5671 6856 6983
Size: 581 343 377 1935 456 1335 1185 127
Cuts at: 6983 644
Size: 2528
Fragments arranged by size:
2528 1935 1335 1185 581 456 377 343
127
MspA1I CmG'CkG
Cuts at: 1158 2292 3223 4615 4704 5153 5226 5421 5427
Size: 1134 931 1392 89 449 73 195 6
Cuts at: 5427 5451 5787 5978 6292 6652 7665 7910 1158
Size: 24 336 191 314 360 1013 245 2115
Fragments arranged by size:
2115 1392 1134 1013 931 449 360 336
314 245 195 191 89 73 24 6
NaeI GCC'GGC
Cuts at: 4792 5011 7048 4792
Size: 219 2037 6611
Fragments arranged by size:
6611 2037 219
NarI GG'CG_CC
Cuts at: 2651 2885 4739 5220 6298 6546 2651
Size: 234 1854 481 1078 248 4972
Fragments arranged by size:
4972 1854 1078 481 248 234
NcoI C'CATG_G
Cuts at: 107 1940 4691 5573 107
Size: 1833 2751 882 3401
Fragments arranged by size:
3401 2751 1833 882
NgoMIV G'CCGG_C
Cuts at: 4790 5009 7046 4790
Size: 219 2037 6611
Fragments arranged by size:
6611 2037 219
NspI r_CATG'y
Cuts at: 2399 4647 5920 6420 7327 2399
Size: 2248 1273 500 907 3939
Fragments arranged by size:
3939 2248 1273 907 500
PasI CC'CwG_GG
Cuts at: 4776 4776
Size: 8867
PciI A'CATG_T
Cuts at: 7323 7323
Size: 8867
PflMI CCAn_nnn'nTGG
Cuts at: 1745 2295 1745
Size: 550 8317
PfoI T'CCnGG_A
Cuts at: 203 932 1744 5039 5657 5792 203
Size: 729 812 3295 618 135 3278
Fragments arranged by size:
3295 3278 812 729 618 135
PmeI GTTT'AAAC
Cuts at: 5866 5866
Size: 8867
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2469 8006 8038 2437
Size: 32 5537 32 3266
Fragments arranged by size:
5537 3266 32 32
PpuMI rG'GwC_Cy
Cuts at: 2635 3304 4869 5814 2635
Size: 669 1565 945 5688
Fragments arranged by size:
5688 1565 945 669
PshAI GACnn'nnGTC
Cuts at: 3562 3562
Size: 8867
PspOMI G'GGCC_C
Cuts at: 3388 3898 4896 3388
Size: 510 998 7359
Fragments arranged by size:
7359 998 510
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 961 993 961
Size: 32 8835
PstI C_TGCA'G
Cuts at: 2645 2828 4475 4583 5213 5432 5648 5914 6599
Size: 183 1647 108 630 219 216 266 685
Cuts at: 6599 2645
Size: 4913
Fragments arranged by size:
4913 1647 685 630 266 219 216 183
108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8867
PvuII CAG'CTG
Cuts at: 1158 2292 3223 4704 5226 5427 6292 6652 1158
Size: 1134 931 1481 522 201 865 360 3373
Fragments arranged by size:
3373 1481 1134 931 865 522 360 201
RsrII CG'GwC_CG
Cuts at: 7062 7062
Size: 8867
SacI G_AGCT'C
Cuts at: 212 1076 4156 5657 5881 212
Size: 864 3080 1501 224 3198
Fragments arranged by size:
3198 3080 1501 864 224
SacII CC_GC'GG
Cuts at: 4616 4616
Size: 8867
SalI G'TCGA_C
Cuts at: 5639 5904 5639
Size: 265 8602
SapI GCTCTTCn'nnn_
Cuts at: 3356 4663 4933 6890 7100 3356
Size: 1307 270 1957 210 5123
Fragments arranged by size:
5123 1957 1307 270 210
SbfI CC_TGCA'GG
Cuts at: 2645 4475 5914 2645
Size: 1830 1439 5598
Fragments arranged by size:
5598 1830 1439
ScaI AGT'ACT
Cuts at: 2485 8384 2485
Size: 5899 2968
SexAI A'CCwGG_T
Cuts at: 3821 3821
Size: 8867
SfcI C'TryA_G
Cuts at: 33 1658 2641 2824 4471 4579 5209 5428 5644
Size: 1625 983 183 1647 108 630 219 216
Cuts at: 5644 5910 6595 7588 7779 33
Size: 266 685 993 191 1121
Fragments arranged by size:
1647 1625 1121 993 983 685 630 266
219 216 191 183 108
SfoI GGC'GCC
Cuts at: 2652 2886 4740 5221 6299 6547 2652
Size: 234 1854 481 1078 248 4972
Fragments arranged by size:
4972 1854 1078 481 248 234
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8867
SmaI CCC'GGG
Cuts at: 1345 4250 4351 5611 5889 1345
Size: 2905 101 1260 278 4323
Fragments arranged by size:
4323 2905 1260 278 101
SmlI C'TyrA_G
Cuts at: 193 265 3994 4464 5269 5389 5686 6022 6937
Size: 72 3729 470 805 120 297 336 915
Cuts at: 6937 7429 7691 193
Size: 492 262 1369
Fragments arranged by size:
3729 1369 915 805 492 470 336 297
262 120 72
SpeI A'CTAG_T
Cuts at: 67 1281 67
Size: 1214 7653
SphI G_CATG'C
Cuts at: 4647 5920 6420 4647
Size: 1273 500 7094
Fragments arranged by size:
7094 1273 500
StuI AGG'CCT
Cuts at: 3055 3055
Size: 8867
StyI C'CwwG_G
Cuts at: 107 437 624 657 791 1250 1940 2003 2108
Size: 330 187 33 134 459 690 63 105
Cuts at: 2108 3767 4541 4604 4622 4691 5573 5837 6001
Size: 1659 774 63 18 69 882 264 164
Cuts at: 6001 6355 107
Size: 354 2619
Fragments arranged by size:
2619 1659 882 774 690 459 354 330
264 187 164 134 105 69 63 63
33 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 517 2223 2971 3433 3633 6450 6583 7042 7251
Size: 1706 748 462 200 2817 133 459 209
Cuts at: 7251 517
Size: 2133
Fragments arranged by size:
2817 2133 1706 748 462 459 209 200
133
TatI w'GTAC_w
Cuts at: 1493 2276 2483 8382 1493
Size: 783 207 5899 1978
Fragments arranged by size:
5899 1978 783 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1503 1535 3831 3863 4138 4170 8384 8416 1503
Size: 32 2296 32 275 32 4214 32 1954
Fragments arranged by size:
4214 2296 1954 275 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1507 5263 6664 1507
Size: 3756 1401 3710
Fragments arranged by size:
3756 3710 1401
XbaI T'CTAG_A
Cuts at: 5898 5898
Size: 8867
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3774 4305 6362 3774
Size: 531 2057 6279
Fragments arranged by size:
6279 2057 531
XmaI C'CCGG_G
Cuts at: 1343 4248 4349 5609 5887 1343
Size: 2905 101 1260 278 4323
Fragments arranged by size:
4323 2905 1260 278 101
XmnI GAAnn'nnTTC
Cuts at: 800 814 2365 4483 800
Size: 14 1551 2118 5184
Fragments arranged by size:
5184 2118 1551 14
ZraI GAC'GTC
Cuts at: 2898 2898
Size: 8867
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AlfI AloI AscI BaeI BfrBI BmtI BsiWI
BsrGI BssHII BstEII BstZ17I ClaI EcoRV FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspXI SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI