Restriction map for
pF1KA0820
(Circular) (Six-base) MAPSORT of: pF1KA0820.seq Check: 8793 from: 1 to: 5690
pF1KA0820 5690 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 944 944
Size: 5690
AccI GT'mk_AC
Cuts at: 1883 1934 2075 2728 1883
Size: 51 141 653 4845
Fragments arranged by size:
4845 653 141 51
Acc65I G'GTAC_C
Cuts at: 2706 2706
Size: 5690
AclI AA'CG_TT
Cuts at: 2153 5530 2153
Size: 3377 2313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 688 978 2183 3515 3947 4694 4962 688
Size: 290 1205 1332 432 747 268 1416
Fragments arranged by size:
1416 1332 1205 747 432 290 268
AflIII A'CryG_T
Cuts at: 161 489 491 2084 3042 4146 5123 161
Size: 328 2 1593 958 1104 977 728
Fragments arranged by size:
1593 1104 977 958 728 328 2
AgeI A'CCGG_T
Cuts at: 4078 4078
Size: 5690
AleI CACnn'nnGTG
Cuts at: 1499 1499
Size: 5690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1868 1868
Size: 5690
AlwNI CAG_nnn'CTG
Cuts at: 1463 2467 2995 4562 1463
Size: 1004 528 1567 2591
Fragments arranged by size:
2591 1567 1004 528
ApaI G_GGCC'C
Cuts at: 2601 2601
Size: 5690
ApaLI G'TGCA_C
Cuts at: 4460 4460
Size: 5690
ApoI r'AATT_y
Cuts at: 376 1053 2169 2694 5043 5299 376
Size: 677 1116 525 2349 256 767
Fragments arranged by size:
2349 1116 767 677 525 256
AseI AT'TA_AT
Cuts at: 20 467 1411 20
Size: 447 944 4299
Fragments arranged by size:
4299 944 447
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
AvaI C'yCGr_G
Cuts at: 250 281 2710 250
Size: 31 2429 3230
Fragments arranged by size:
3230 2429 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2088 2121 2088
Size: 33 5657
BamHI G'GATC_C
Cuts at: 2715 2715
Size: 5690
BanI G'GyrC_C
Cuts at: 2337 2706 3120 3368 3403 2337
Size: 369 414 248 35 4624
Fragments arranged by size:
4624 414 369 248 35
BanII G_rGCy'C
Cuts at: 244 2180 2502 2568 2601 2704 3734 244
Size: 1936 322 66 33 103 1030 2200
Fragments arranged by size:
2200 1936 1030 322 103 66 33
BbeI G_GCGC'C
Cuts at: 3124 3372 3124
Size: 248 5442
BbsI GAAGACnn'nnnn_
Cuts at: 1073 1087 1522 1586 1073
Size: 14 435 64 5177
Fragments arranged by size:
5177 435 64 14
BciVI GTATCCnnnnn_n'
Cuts at: 972 1708 2116 2357 3588 4349 5690 972
Size: 736 408 241 1231 761 1341 972
Fragments arranged by size:
1341 1231 972 761 736 408 241
BclI T'GATC_A
Cuts at: 2144 3210 2144
Size: 1066 4624
BglI GCCn_nnn'nGGC
Cuts at: 865 865
Size: 5690
BglII A'GATC_T
Cuts at: 2112 3205 5242 2112
Size: 1093 2037 2560
Fragments arranged by size:
2560 2037 1093
BlpI GC'TnA_GC
Cuts at: 1604 2234 2243 2802 1604
Size: 630 9 559 4492
Fragments arranged by size:
4492 630 559 9
Bme1580I G_kGCm'C
Cuts at: 2601 3315 3408 4464 2601
Size: 714 93 1056 3827
Fragments arranged by size:
3827 1056 714 93
BmrI ACTGGGnnnn_n'
Cuts at: 2600 2902 2983 3318 5462 2600
Size: 302 81 335 2144 2828
Fragments arranged by size:
2828 2144 335 302 81
BmtI G_CTAG'C
Cuts at: 676 676
Size: 5690
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4057 4057
Size: 5690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 617 1304 2330 2489 214
Size: 403 687 1026 159 3415
Fragments arranged by size:
3415 1026 687 403 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 677 1924 2866 3745 4237 4535 424
Size: 253 1247 942 879 492 298 1579
Fragments arranged by size:
1579 1247 942 879 492 298 253
BsaI GGTCTCn'nnnn_
Cuts at: 2050 2616 5394 2050
Size: 566 2778 2346
Fragments arranged by size:
2778 2346 566
BsaAI yAC'GTr
Cuts at: 492 801 3043 492
Size: 309 2242 3139
Fragments arranged by size:
3139 2242 309
BsaBI GATnn'nnATC
Cuts at: 6 3204 5241 6
Size: 3198 2037 455
Fragments arranged by size:
3198 2037 455
BsaHI Gr'CG_yC
Cuts at: 3121 3369 4071 5567 3121
Size: 248 702 1496 3244
Fragments arranged by size:
3244 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2879 3400 4078 4352 4499 4910 2879
Size: 521 678 274 147 411 3659
Fragments arranged by size:
3659 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 142 1626 2378 2492 142
Size: 1484 752 114 3340
Fragments arranged by size:
3340 1484 752 114
BseYI C'CCAG_C
Cuts at: 174 1963 2542 3115 3873 4450 4946 174
Size: 1789 579 573 758 577 496 918
Fragments arranged by size:
1789 918 758 579 577 573 496
BsiEI CG_ry'CG
Cuts at: 104 2454 3232 3278 4486 104
Size: 2350 778 46 1208 1308
Fragments arranged by size:
2350 1308 1208 778 46
BsiHKAI G_wGCw'C
Cuts at: 244 251 2180 2430 2704 3482 3672 4464 244
Size: 7 1929 250 274 778 190 792 1470
Fragments arranged by size:
1929 1470 792 778 274 250 190 7
Bsp1286I G_dGCh'C
Cuts at: 244 251 2180 2430 2502 2568 2601 2704 3315
Size: 7 1929 250 72 66 33 103 611
Cuts at: 3315 3408 3482 3672 3734 4464 244
Size: 93 74 190 62 730 1470
Fragments arranged by size:
1929 1470 730 611 250 190 103 93
74 72 66 62 33 7
BspEI T'CCGG_A
Cuts at: 4910 4910
Size: 5690
BspHI T'CATG_A
Cuts at: 1206 1694 4866 1206
Size: 488 3172 2030
Fragments arranged by size:
3172 2030 488
BspMI ACCTGCnnnn'nnnn_
Cuts at: 944 2369 2740 2954 3256 3637 944
Size: 1425 371 214 302 381 2997
Fragments arranged by size:
2997 1425 381 371 302 214
BsrBI CCG'CTC
Cuts at: 50 3982 4036 5520 5685 50
Size: 3932 54 1484 165 55
Fragments arranged by size:
3932 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 360 1090 3602 360
Size: 730 2512 2448
Fragments arranged by size:
2512 2448 730
BsrFI r'CCGG_y
Cuts at: 232 3688 3869 4078 232
Size: 3456 181 209 1844
Fragments arranged by size:
3456 1844 209 181
BssSI C'ACGA_G
Cuts at: 600 3961 4319 600
Size: 3361 358 1971
Fragments arranged by size:
3361 1971 358
BstAPI GCAn_nnn'nTGC
Cuts at: 346 3067 346
Size: 2721 2969
BstBI TT'CG_AA
Cuts at: 2265 4051 2265
Size: 1786 3904
BstEII G'GTnAC_C
Cuts at: 2381 2381
Size: 5690
BstXI CCAn_nnnn'nTGG
Cuts at: 1443 1443
Size: 5690
BstYI r'GATC_y
Cuts at: 1 1392 2112 2715 3182 3205 3540 3786 4798
Size: 1391 720 603 467 23 335 246 1012
Cuts at: 4798 4884 4896 5242 1
Size: 86 12 346 449
Fragments arranged by size:
1391 1012 720 603 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1884 1884
Size: 5690
BtgI C'CryG_G
Cuts at: 107 212 2027 107
Size: 105 1815 3770
Fragments arranged by size:
3770 1815 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3640 3821 3984 3640
Size: 181 163 5346
Fragments arranged by size:
5346 181 163
BtsI GCAGTG_nn'
Cuts at: 2556 2933 2556
Size: 377 5313
DraI TTT'AAA
Cuts at: 2689 2689
Size: 5690
DraIII CAC_nnn'GTG
Cuts at: 2425 2425
Size: 5690
DrdI GACnn_nn'nnGTC
Cuts at: 1552 3396 4254 1552
Size: 1844 858 2988
Fragments arranged by size:
2988 1844 858
EaeI y'GGCC_r
Cuts at: 222 2318 2451 3275 3449 3840 3867 5212 222
Size: 2096 133 824 174 391 27 1345 700
Fragments arranged by size:
2096 1345 824 700 391 174 133 27
EagI C'GGCC_G
Cuts at: 2451 3275 2451
Size: 824 4866
EarI CTCTTCn'nnn_
Cuts at: 791 1126 1257 1378 3713 3923 791
Size: 335 131 121 2335 210 2558
Fragments arranged by size:
2558 2335 335 210 131 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 3898 4208 4354 5500 2647
Size: 1251 310 146 1146 2837
Fragments arranged by size:
2837 1251 1146 310 146
EcoICRI GAG'CTC
Cuts at: 242 2178 2702 242
Size: 1936 524 3230
Fragments arranged by size:
3230 1936 524
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 214 617 688 978 1304 2183 2330 2489 3515
Size: 403 71 290 326 879 147 159 1026
Cuts at: 3515 3947 4694 4962 214
Size: 432 747 268 942
Fragments arranged by size:
1026 942 879 747 432 403 326 290
268 159 147 71
EcoNI CCTnn'n_nnAGG
Cuts at: 940 2221 940
Size: 1281 4409
EcoO109I rG'GnC_Cy
Cuts at: 945 1372 2597 2598 2829 945
Size: 427 1225 1 231 3806
Fragments arranged by size:
3806 1225 427 231 1
EcoRI G'AATT_C
Cuts at: 2694 2694
Size: 5690
EcoRV GAT'ATC
Cuts at: 561 561
Size: 5690
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 418 1866 2308 3709 418
Size: 1448 442 1401 2399
Fragments arranged by size:
2399 1448 1401 442
FspI TGC'GCA
Cuts at: 3471 3471
Size: 5690
HaeII r_GCGC'y
Cuts at: 175 233 2250 3124 3372 4394 5373 175
Size: 58 2017 874 248 1022 979 492
Fragments arranged by size:
2017 1022 979 874 492 248 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 1377 1409 1602 1634 1952 1984 3532
Size: 32 341 32 193 32 318 32 1548
Cuts at: 3532 3562 3564 3594 1004
Size: 30 2 30 3100
Fragments arranged by size:
3100 1548 341 318 193 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1935 2729 1935
Size: 794 4896
HindIII A'AGCT_T
Cuts at: 1740 1740
Size: 5690
KasI G'GCGC_C
Cuts at: 3120 3368 3120
Size: 248 5442
KpnI G_GTAC'C
Cuts at: 2710 2710
Size: 5690
MfeI C'AATT_G
Cuts at: 2038 2038
Size: 5690
MluI A'CGCG_T
Cuts at: 161 5123 161
Size: 4962 728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1623 4361 4545 1623
Size: 2738 184 2768
Fragments arranged by size:
2768 2738 184
MscI TGG'CCA
Cuts at: 3451 3451
Size: 5690
MslI CAynn'nnrTG
Cuts at: 1499 3679 3806 1499
Size: 2180 127 3383
Fragments arranged by size:
3383 2180 127
MspA1I CmG'CkG
Cuts at: 141 1940 2801 3115 3475 4488 4733 141
Size: 1799 861 314 360 1013 245 1098
Fragments arranged by size:
1799 1098 1013 861 360 314 245
NaeI GCC'GGC
Cuts at: 234 3871 234
Size: 3637 2053
NarI GG'CG_CC
Cuts at: 3121 3369 3121
Size: 248 5442
NcoI C'CATG_G
Cuts at: 107 2027 107
Size: 1920 3770
NgoMIV G'CCGG_C
Cuts at: 232 3869 232
Size: 3637 2053
NheI G'CTAG_C
Cuts at: 672 672
Size: 5690
NspI r_CATG'y
Cuts at: 1788 1916 2088 2743 3243 4150 1788
Size: 128 172 655 500 907 3328
Fragments arranged by size:
3328 907 655 500 172 128
PciI A'CATG_T
Cuts at: 2084 4146 2084
Size: 2062 3628
PfoI T'CCnGG_A
Cuts at: 2359 2359
Size: 5690
PmeI GTTT'AAAC
Cuts at: 2689 2689
Size: 5690
PmlI CAC'GTG
Cuts at: 492 492
Size: 5690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4829 4861 4829
Size: 32 5658
PpuMI rG'GwC_Cy
Cuts at: 945 945
Size: 5690
PspOMI G'GGCC_C
Cuts at: 2597 2597
Size: 5690
PspXI vC'TCGA_Gb
Cuts at: 281 281
Size: 5690
PstI C_TGCA'G
Cuts at: 158 323 941 1100 2737 3422 158
Size: 165 618 159 1637 685 2426
Fragments arranged by size:
2426 1637 685 618 165 159
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5690
PvuII CAG'CTG
Cuts at: 1940 3115 3475 1940
Size: 1175 360 4155
Fragments arranged by size:
4155 1175 360
RsrII CG'GwC_CG
Cuts at: 3885 3885
Size: 5690
SacI G_AGCT'C
Cuts at: 244 2180 2704 244
Size: 1936 524 3230
Fragments arranged by size:
3230 1936 524
SalI G'TCGA_C
Cuts at: 1933 2727 1933
Size: 794 4896
SapI GCTCTTCn'nnn_
Cuts at: 3713 3923 3713
Size: 210 5480
SbfI CC_TGCA'GG
Cuts at: 2737 2737
Size: 5690
ScaI AGT'ACT
Cuts at: 5207 5207
Size: 5690
SexAI A'CCwGG_T
Cuts at: 1526 1526
Size: 5690
SfcI C'TryA_G
Cuts at: 33 154 319 937 1006 1096 1985 2733 3418
Size: 121 165 618 69 90 889 748 685
Cuts at: 3418 4411 4602 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 889 748 685 618 191 165
121 90 69
SfoI GGC'GCC
Cuts at: 3122 3370 3122
Size: 248 5442
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5690
SmaI CCC'GGG
Cuts at: 2712 2712
Size: 5690
SmlI C'TyrA_G
Cuts at: 250 281 403 692 1903 2845 3760 4252 4514
Size: 31 122 289 1211 942 915 492 262
Cuts at: 4514 250
Size: 1426
Fragments arranged by size:
1426 1211 942 915 492 289 262 122
31
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5690
SphI G_CATG'C
Cuts at: 2743 3243 2743
Size: 500 5190
StuI AGG'CCT
Cuts at: 2589 2589
Size: 5690
StyI C'CwwG_G
Cuts at: 107 1793 2027 2292 2824 3178 107
Size: 1686 234 265 532 354 2619
Fragments arranged by size:
2619 1686 532 354 265 234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 932 3273 3406 3865 4074 932
Size: 2341 133 459 209 2548
Fragments arranged by size:
2548 2341 459 209 133
TatI w'GTAC_w
Cuts at: 1781 5205 1781
Size: 3424 2266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1637 1669 5207 5239 1637
Size: 32 3538 32 2088
Fragments arranged by size:
3538 2088 32 32
Tth111I GACn'n_nGTC
Cuts at: 3487 3487
Size: 5690
XbaI T'CTAG_A
Cuts at: 765 2721 765
Size: 1956 3734
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3185 3185
Size: 5690
XhoI C'TCGA_G
Cuts at: 250 281 250
Size: 31 5659
XmaI C'CCGG_G
Cuts at: 2710 2710
Size: 5690
XmnI GAAnn'nnTTC
Cuts at: 880 1214 880
Size: 334 5356
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BbvCI
BcgI BfrBI BmgBI Bpu10I BsaXI BsgI BsiWI BsmI
BsmBI BsrGI BssHII Bsu36I ClaI CspCI FseI FspAI
HpaI NdeI NotI NruI NsiI PacI PasI PflMI
PshAI PsiI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI