Restriction map for
pF1KA0822
(Circular) (Six-base) MAPSORT of: pF1KA0822.seq Check: 1308 from: 1 to: 7856
pF1KA0822 7856 bp
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3507 3507
Size: 7856
AatII G_ACGT'C
Cuts at: 1401 1401
Size: 7856
AccI GT'mk_AC
Cuts at: 341 2629 3840 4321 4741 4894 341
Size: 2288 1211 481 420 153 3303
Fragments arranged by size:
3303 2288 1211 481 420 153
Acc65I G'GTAC_C
Cuts at: 4872 4872
Size: 7856
AclI AA'CG_TT
Cuts at: 7696 7696
Size: 7856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 999 1438 1980 2371 3581 4240 5681 6113
Size: 508 439 542 391 1210 659 1441 432
Cuts at: 6113 6466 7128 491
Size: 353 662 1219
Fragments arranged by size:
1441 1219 1210 662 659 542 508 439
432 391 353
AflII C'TTAA_G
Cuts at: 2121 2121
Size: 7856
AflIII A'CryG_T
Cuts at: 5208 7008 7289 5208
Size: 1800 281 5775
Fragments arranged by size:
5775 1800 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 883 915 883
Size: 32 7824
AlwNI CAG_nnn'CTG
Cuts at: 721 3198 4125 4429 4785 5161 6599 721
Size: 2477 927 304 356 376 1438 1978
Fragments arranged by size:
2477 1978 1438 927 376 356 304
ApaLI G'TGCA_C
Cuts at: 6694 6694
Size: 7856
ApoI r'AATT_y
Cuts at: 213 451 1257 1311 1370 1525 2257 3695 3795
Size: 238 806 54 59 155 732 1438 100
Cuts at: 3795 4510 4860 7209 7465 213
Size: 715 350 2349 256 604
Fragments arranged by size:
2349 1438 806 732 715 604 350 256
238 155 100 59 54
AseI AT'TA_AT
Cuts at: 20 659 2375 2590 20
Size: 639 1716 215 5286
Fragments arranged by size:
5286 1716 639 215
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7856
AvaI C'yCGr_G
Cuts at: 4336 4876 4336
Size: 540 7316
AvrII C'CTAG_G
Cuts at: 2298 2298
Size: 7856
BamHI G'GATC_C
Cuts at: 1929 3681 4881 1929
Size: 1752 1200 4904
Fragments arranged by size:
4904 1752 1200
BanI G'GyrC_C
Cuts at: 4396 4872 5286 5534 5569 4396
Size: 476 414 248 35 6683
Fragments arranged by size:
6683 476 414 248 35
BanII G_rGCy'C
Cuts at: 2080 3141 4568 4769 4870 5900 2080
Size: 1061 1427 201 101 1030 4036
Fragments arranged by size:
4036 1427 1061 1030 201 101
BbeI G_GCGC'C
Cuts at: 5290 5538 5290
Size: 248 7608
BbsI GAAGACnn'nnnn_
Cuts at: 877 1005 3219 3275 4240 877
Size: 128 2214 56 965 4493
Fragments arranged by size:
4493 2214 965 128 56
BbvCI CC'TCA_GC
Cuts at: 4153 4169 4430 4153
Size: 16 261 7579
Fragments arranged by size:
7579 261 16
BciVI GTATCCnnnnn_n'
Cuts at: 5754 6810 7856 5754
Size: 1056 1046 5754
Fragments arranged by size:
5754 1056 1046
BclI T'GATC_A
Cuts at: 1452 2735 5376 1452
Size: 1283 2641 3932
Fragments arranged by size:
3932 2641 1283
BfrBI ATG'CAT
Cuts at: 741 826 1267 2815 741
Size: 85 441 1548 5782
Fragments arranged by size:
5782 1548 441 85
BglI GCCn_nnn'nGGC
Cuts at: 4369 4369
Size: 7856
BglII A'GATC_T
Cuts at: 973 1608 5371 7408 973
Size: 635 3763 2037 1421
Fragments arranged by size:
3763 2037 1421 635
BlpI GC'TnA_GC
Cuts at: 4968 4968
Size: 7856
Bme1580I G_kGCm'C
Cuts at: 4401 5481 5574 6698 4401
Size: 1080 93 1124 5559
Fragments arranged by size:
5559 1124 1080 93
BmrI ACTGGGnnnn_n'
Cuts at: 975 1998 2929 4294 5068 5149 5484 7628 975
Size: 1023 931 1365 774 81 335 2144 1203
Fragments arranged by size:
2144 1365 1203 1023 931 774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1858 6223 1858
Size: 4365 3491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1505 4019 4081 4147 4552 4768 4783 1505
Size: 2514 62 66 405 216 15 4578
Fragments arranged by size:
4578 2514 405 216 66 62 15
Bpu10I CC'TnA_GC
Cuts at: 4153 4169 4276 4430 4153
Size: 16 107 154 7579
Fragments arranged by size:
7579 154 107 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4186 5032 5911 6625 6923 4186
Size: 846 879 714 298 5119
Fragments arranged by size:
5119 879 846 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1439 7560 1439
Size: 6121 1735
BsaAI yAC'GTr
Cuts at: 1458 3351 5209 1458
Size: 1893 1858 4105
Fragments arranged by size:
4105 1893 1858
BsaBI GATnn'nnATC
Cuts at: 6 1484 2288 4808 5370 7407 6
Size: 1478 804 2520 562 2037 455
Fragments arranged by size:
2520 2037 1478 804 562 455
BsaHI Gr'CG_yC
Cuts at: 1398 5287 5535 6237 7733 1398
Size: 3889 248 702 1496 1521
Fragments arranged by size:
3889 1521 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1786 3160 4227 5045 5566 6244 6655 6802 7076
Size: 1374 1067 818 521 678 411 147 274
Cuts at: 7076 1786
Size: 2566
Fragments arranged by size:
2566 1374 1067 818 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 697 727 697
Size: 30 7826
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 718 1105 2666 4614 4735 4813 4822 718
Size: 387 1561 1948 121 78 9 3752
Fragments arranged by size:
3752 1948 1561 387 121 78 9
BseYI C'CCAG_C
Cuts at: 1229 3146 4785 5281 6039 6704 7112 1229
Size: 1917 1639 496 758 665 408 1973
Fragments arranged by size:
1973 1917 1639 758 665 496 408
BsiEI CG_ry'CG
Cuts at: 104 4325 5398 5444 6674 104
Size: 4221 1073 46 1230 1286
Fragments arranged by size:
4221 1286 1230 1073 46
BsiHKAI G_wGCw'C
Cuts at: 560 2789 3141 4769 4870 5648 5838 6698 560
Size: 2229 352 1628 101 778 190 860 1718
Fragments arranged by size:
2229 1718 1628 860 778 352 190 101
BsmI GAATG_Cn'
Cuts at: 545 741 545
Size: 196 7660
Bsp1286I G_dGCh'C
Cuts at: 560 2080 2789 3141 4401 4568 4769 4870 5481
Size: 1520 709 352 1260 167 201 101 611
Cuts at: 5481 5574 5648 5838 5900 6698 560
Size: 93 74 190 62 798 1718
Fragments arranged by size:
1718 1520 1260 798 709 611 352 201
190 167 101 93 74 62
BspEI T'CCGG_A
Cuts at: 3160 3160
Size: 7856
BspHI T'CATG_A
Cuts at: 1497 3341 6288 1497
Size: 1844 2947 3065
Fragments arranged by size:
3065 2947 1844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 397 3507 4535 4743 4906 5120 5422 5803 397
Size: 3110 1028 208 163 214 302 381 2450
Fragments arranged by size:
3110 2450 1028 381 302 214 208 163
BsrBI CCG'CTC
Cuts at: 50 4043 6148 6202 7686 7851 50
Size: 3993 2105 54 1484 165 55
Fragments arranged by size:
3993 2105 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 217 2387 2916 3101 3827 5768 217
Size: 2170 529 185 726 1941 2305
Fragments arranged by size:
2305 2170 1941 726 529 185
BsrFI r'CCGG_y
Cuts at: 1096 5854 6035 1096
Size: 4758 181 2917
Fragments arranged by size:
4758 2917 181
BsrGI T'GTAC_A
Cuts at: 1154 3576 1154
Size: 2422 5434
BssSI C'ACGA_G
Cuts at: 3903 6127 6835 3903
Size: 2224 708 4924
Fragments arranged by size:
4924 2224 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2911 5233 2911
Size: 2322 5534
BstBI TT'CG_AA
Cuts at: 3266 3458 6217 3266
Size: 192 2759 4905
Fragments arranged by size:
4905 2759 192
BstXI CCAn_nnnn'nTGG
Cuts at: 297 297
Size: 7856
BstYI r'GATC_y
Cuts at: 1 973 1608 1929 2277 3681 4580 4881 5348
Size: 972 635 321 348 1404 899 301 467
Cuts at: 5348 5371 5706 5952 6258 6270 6356 7408 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1404 1052 972 899 635 467 449 348
335 321 306 301 246 86 23 12
BstZ17I GTA'TAC
Cuts at: 342 2630 342
Size: 2288 5568
Bsu36I CC'TnA_GG
Cuts at: 4082 4585 4082
Size: 503 7353
BtgI C'CryG_G
Cuts at: 290 2633 290
Size: 2343 5513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5806 5987 6150 5806
Size: 181 163 7512
Fragments arranged by size:
7512 181 163
BtsI GCAGTG_nn'
Cuts at: 280 1640 1678 2581 5099 280
Size: 1360 38 903 2518 3037
Fragments arranged by size:
3037 2518 1360 903 38
ClaI AT'CG_AT
Cuts at: 3119 3119
Size: 7856
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3492 3527 3492
Size: 35 7821
DraI TTT'AAA
Cuts at: 4855 4855
Size: 7856
DraIII CAC_nnn'GTG
Cuts at: 696 2870 696
Size: 2174 5682
DrdI GACnn_nn'nnGTC
Cuts at: 1397 5562 6906 1397
Size: 4165 1344 2347
Fragments arranged by size:
4165 2347 1344
EaeI y'GGCC_r
Cuts at: 4451 5441 5615 6006 6033 7378 4451
Size: 990 174 391 27 1345 4929
Fragments arranged by size:
4929 1345 990 391 174 27
EagI C'GGCC_G
Cuts at: 5441 5441
Size: 7856
EarI CTCTTCn'nnn_
Cuts at: 109 650 1999 2085 2469 2621 3641 3856 4838
Size: 541 1349 86 384 152 1020 215 982
Cuts at: 4838 5879 6089 109
Size: 1041 210 1876
Fragments arranged by size:
1876 1349 1041 1020 982 541 384 215
210 152 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2047 6064 6806 6952 7666 2047
Size: 4017 742 146 714 2237
Fragments arranged by size:
4017 2237 742 714 146
EcoICRI GAG'CTC
Cuts at: 3139 4767 4868 3139
Size: 1628 101 6127
Fragments arranged by size:
6127 1628 101
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 999 1438 1505 1980 2371 3581 4019 4081
Size: 508 439 67 475 391 1210 438 62
Cuts at: 4081 4147 4240 4552 4768 4783 5681 6113 6466
Size: 66 93 312 216 15 898 432 353
Cuts at: 6466 7128 491
Size: 662 1219
Fragments arranged by size:
1219 1210 898 662 508 475 439 438
432 391 353 312 216 93 67 66
62 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 4750 4837 2297
Size: 2453 87 5316
Fragments arranged by size:
5316 2453 87
EcoO109I rG'GnC_Cy
Cuts at: 4995 4995
Size: 7856
EcoRI G'AATT_C
Cuts at: 213 451 1257 1525 3795 4860 213
Size: 238 806 268 2270 1065 3209
Fragments arranged by size:
3209 2270 1065 806 268 238
EcoRV GAT'ATC
Cuts at: 2116 2116
Size: 7856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 4284 5875 2239
Size: 2045 1591 4220
Fragments arranged by size:
4220 2045 1591
FspI TGC'GCA
Cuts at: 2526 5637 2526
Size: 3111 4745
HaeII r_GCGC'y
Cuts at: 1519 3199 5290 5538 6768 7539 1519
Size: 1680 2091 248 1230 771 1836
Fragments arranged by size:
2091 1836 1680 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 697 729 2100 2132 2504 2536 2830 2862 3666
Size: 32 1371 32 372 32 294 32 804
Cuts at: 3666 3698 4303 4335 4439 4471 5698 5728 5730
Size: 32 605 32 104 32 1227 30 2
Cuts at: 5730 5760 697
Size: 30 2793
Fragments arranged by size:
2793 1371 1227 804 605 372 294 104
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 136 1676 4322 4895 136
Size: 1540 2646 573 3097
Fragments arranged by size:
3097 2646 1540 573
HindIII A'AGCT_T
Cuts at: 1191 1744 2124 4676 1191
Size: 553 380 2552 4371
Fragments arranged by size:
4371 2552 553 380
KasI G'GCGC_C
Cuts at: 5286 5534 5286
Size: 248 7608
KpnI G_GTAC'C
Cuts at: 4876 4876
Size: 7856
MfeI C'AATT_G
Cuts at: 2528 2528
Size: 7856
MluI A'CGCG_T
Cuts at: 7289 7289
Size: 7856
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1886 4518 6615 6799 1886
Size: 2632 2097 184 2943
Fragments arranged by size:
2943 2632 2097 184
MscI TGG'CCA
Cuts at: 4453 5617 4453
Size: 1164 6692
MslI CAynn'nnrTG
Cuts at: 581 2866 3106 3340 3988 5845 5972 581
Size: 2285 240 234 648 1857 127 2465
Fragments arranged by size:
2465 2285 1857 648 240 234 127
MspA1I CmG'CkG
Cuts at: 3836 4218 4967 5281 5641 6425 6670 3836
Size: 382 749 314 360 784 245 5022
Fragments arranged by size:
5022 784 749 382 360 314 245
NaeI GCC'GGC
Cuts at: 6037 6037
Size: 7856
NarI GG'CG_CC
Cuts at: 5287 5535 5287
Size: 248 7608
NcoI C'CATG_G
Cuts at: 290 2633 290
Size: 2343 5513
NgoMIV G'CCGG_C
Cuts at: 6035 6035
Size: 7856
NsiI A_TGCA'T
Cuts at: 743 828 1269 2817 743
Size: 85 441 1548 5782
Fragments arranged by size:
5782 1548 441 85
NspI r_CATG'y
Cuts at: 2909 3276 4909 5409 7012 2909
Size: 367 1633 500 1603 3753
Fragments arranged by size:
3753 1633 1603 500 367
PciI A'CATG_T
Cuts at: 7008 7008
Size: 7856
PflMI CCAn_nnn'nTGG
Cuts at: 1921 2010 4018 4419 1921
Size: 89 2008 401 5358
Fragments arranged by size:
5358 2008 401 89
PfoI T'CCnGG_A
Cuts at: 2697 4760 2697
Size: 2063 5793
PmeI GTTT'AAAC
Cuts at: 4855 4855
Size: 7856
PmlI CAC'GTG
Cuts at: 1458 1458
Size: 7856
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2444 2476 6302 6334 2444
Size: 32 3826 32 3966
Fragments arranged by size:
3966 3826 32 32
PsiI TTA'TAA
Cuts at: 769 970 2852 3128 4640 769
Size: 201 1882 276 1512 3985
Fragments arranged by size:
3985 1882 1512 276 201
PstI C_TGCA'G
Cuts at: 4211 4903 5588 4211
Size: 692 685 6479
Fragments arranged by size:
6479 692 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7856
PvuII CAG'CTG
Cuts at: 3836 4218 5281 5641 3836
Size: 382 1063 360 6051
Fragments arranged by size:
6051 1063 382 360
RsrII CG'GwC_CG
Cuts at: 6051 6051
Size: 7856
SacI G_AGCT'C
Cuts at: 3141 4769 4870 3141
Size: 1628 101 6127
Fragments arranged by size:
6127 1628 101
SalI G'TCGA_C
Cuts at: 4320 4893 4320
Size: 573 7283
SapI GCTCTTCn'nnn_
Cuts at: 650 2085 4838 5879 6089 650
Size: 1435 2753 1041 210 2417
Fragments arranged by size:
2753 2417 1435 1041 210
SbfI CC_TGCA'GG
Cuts at: 4903 4903
Size: 7856
ScaI AGT'ACT
Cuts at: 7373 7373
Size: 7856
SfcI C'TryA_G
Cuts at: 33 4207 4899 5584 6552 6743 33
Size: 4174 692 685 968 191 1146
Fragments arranged by size:
4174 1146 968 692 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 4369 4369
Size: 7856
SfoI GGC'GCC
Cuts at: 5288 5536 5288
Size: 248 7608
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7856
SmaI CCC'GGG
Cuts at: 4878 4878
Size: 7856
SmlI C'TyrA_G
Cuts at: 2121 4201 5011 5926 6640 6902 2121
Size: 2080 810 915 714 262 3075
Fragments arranged by size:
3075 2080 915 810 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7856
SphI G_CATG'C
Cuts at: 2909 4909 5409 2909
Size: 2000 500 5356
Fragments arranged by size:
5356 2000 500
StuI AGG'CCT
Cuts at: 865 865
Size: 7856
StyI C'CwwG_G
Cuts at: 290 1175 1530 2055 2298 2633 4772 4990 5344
Size: 885 355 525 243 335 2139 218 354
Cuts at: 5344 290
Size: 2802
Fragments arranged by size:
2802 2139 885 525 355 354 335 243
218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3131 4426 4460 4777 5439 5572 6031 6240 3131
Size: 1295 34 317 662 133 459 209 4747
Fragments arranged by size:
4747 1295 662 459 317 209 133 34
TatI w'GTAC_w
Cuts at: 1154 2406 2988 3010 3215 3576 4724 7371 1154
Size: 1252 582 22 205 361 1148 2647 1639
Fragments arranged by size:
2647 1639 1252 1148 582 361 205 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7373 7405 7373
Size: 32 7824
Tth111I GACn'n_nGTC
Cuts at: 4239 5653 4239
Size: 1414 6442
XbaI T'CTAG_A
Cuts at: 4887 4887
Size: 7856
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4416 5351 4416
Size: 935 6921
XmaI C'CCGG_G
Cuts at: 4876 4876
Size: 7856
XmnI GAAnn'nnTTC
Cuts at: 1087 2929 3758 1087
Size: 1842 829 5185
Fragments arranged by size:
5185 1842 829
ZraI GAC'GTC
Cuts at: 1399 1399
Size: 7856
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI AleI AlfI ApaI AscI BaeI
BcgI BmgBI BmtI BsgI BsiWI BsmBI BssHII BstEII
FseI FspAI HpaI NdeI NheI NotI NruI PacI
PasI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI SwaI XhoI