Restriction map for
pF1KA0829
(Circular) (Six-base) MAPSORT of: pF1KA0829.seq Check: 780 from: 1 to: 6803
pF1KA0829 6803 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 519 519
Size: 6803
AccI GT'mk_AC
Cuts at: 645 2596 3499 3841 645
Size: 1951 903 342 3607
Fragments arranged by size:
3607 1951 903 342
Acc65I G'GTAC_C
Cuts at: 3819 3819
Size: 6803
AclI AA'CG_TT
Cuts at: 6643 6643
Size: 6803
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1739 1927 4628 5060 5807 6075 1375
Size: 364 188 2701 432 747 268 2103
Fragments arranged by size:
2701 2103 747 432 364 268 188
AflII C'TTAA_G
Cuts at: 2658 3016 2658
Size: 358 6445
AflIII A'CryG_T
Cuts at: 1475 3562 4155 5259 6236 1475
Size: 2087 593 1104 977 2042
Fragments arranged by size:
2087 2042 1104 977 593
AgeI A'CCGG_T
Cuts at: 5191 5191
Size: 6803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 406 406
Size: 6803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2045 2077 2045
Size: 32 6771
AlwNI CAG_nnn'CTG
Cuts at: 4108 5675 4108
Size: 1567 5236
ApaI G_GGCC'C
Cuts at: 1512 1512
Size: 6803
ApaLI G'TGCA_C
Cuts at: 5573 5573
Size: 6803
ApoI r'AATT_y
Cuts at: 303 892 1216 1581 3076 3418 3697 3807 6156
Size: 589 324 365 1495 342 279 110 2349
Cuts at: 6156 6412 303
Size: 256 694
Fragments arranged by size:
2349 1495 694 589 365 342 324 279
256 110
AseI AT'TA_AT
Cuts at: 20 3226 20
Size: 3206 3597
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6803
AvaI C'yCGr_G
Cuts at: 2528 3823 2528
Size: 1295 5508
BamHI G'GATC_C
Cuts at: 2302 3828 2302
Size: 1526 5277
BanI G'GyrC_C
Cuts at: 3819 4233 4481 4516 3819
Size: 414 248 35 6106
Fragments arranged by size:
6106 414 248 35
BanII G_rGCy'C
Cuts at: 1512 2198 2361 3817 4847 1512
Size: 686 163 1456 1030 3468
Fragments arranged by size:
3468 1456 1030 686 163
BbeI G_GCGC'C
Cuts at: 4237 4485 4237
Size: 248 6555
BbsI GAAGACnn'nnnn_
Cuts at: 1459 1635 3161 1459
Size: 176 1526 5101
Fragments arranged by size:
5101 1526 176
BciVI GTATCCnnnnn_n'
Cuts at: 2413 3237 3760 4701 5462 6803 2413
Size: 824 523 941 761 1341 2413
Fragments arranged by size:
2413 1341 941 824 761 523
BclI T'GATC_A
Cuts at: 1101 1851 4323 1101
Size: 750 2472 3581
Fragments arranged by size:
3581 2472 750
BfrBI ATG'CAT
Cuts at: 2226 2743 2226
Size: 517 6286
BglII A'GATC_T
Cuts at: 744 1806 4318 6355 744
Size: 1062 2512 2037 1192
Fragments arranged by size:
2512 2037 1192 1062
BlpI GC'TnA_GC
Cuts at: 3915 3915
Size: 6803
Bme1580I G_kGCm'C
Cuts at: 1512 4428 4521 5577 1512
Size: 2916 93 1056 2738
Fragments arranged by size:
2916 2738 1056 93
BmgBI CAC'GTC
Cuts at: 1159 1159
Size: 6803
BmrI ACTGGGnnnn_n'
Cuts at: 648 2119 4015 4096 4431 6575 648
Size: 1471 1896 81 335 2144 876
Fragments arranged by size:
2144 1896 1471 876 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2489 5170 2489
Size: 2681 4122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1658 446
Size: 1212 5591
Bpu10I CC'TnA_GC
Cuts at: 3725 3725
Size: 6803
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1622 2574 3979 4858 5350 5648 886
Size: 736 952 1405 879 492 298 2041
Fragments arranged by size:
2041 1405 952 879 736 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1688 6507 1688
Size: 4819 1984
BsaAI yAC'GTr
Cuts at: 1614 3556 4156 1614
Size: 1942 600 4261
Fragments arranged by size:
4261 1942 600
BsaBI GATnn'nnATC
Cuts at: 6 1100 4317 6354 6
Size: 1094 3217 2037 455
Fragments arranged by size:
3217 2037 1094 455
BsaHI Gr'CG_yC
Cuts at: 516 4234 4482 5184 6680 516
Size: 3718 248 702 1496 639
Fragments arranged by size:
3718 1496 702 639 248
BsaWI w'CCGG_w
Cuts at: 3992 4513 5191 5465 5612 6023 3992
Size: 521 678 274 147 411 4772
Fragments arranged by size:
4772 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1218 1248 2045 2075 1218
Size: 30 797 30 5946
Fragments arranged by size:
5946 797 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1625 2271 1625
Size: 646 6157
BseYI C'CCAG_C
Cuts at: 2014 4228 4986 5563 6059 2014
Size: 2214 758 577 496 2758
Fragments arranged by size:
2758 2214 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 1181 1223 2954 3536 366
Size: 815 42 1731 582 3633
Fragments arranged by size:
3633 1731 815 582 42
BsiEI CG_ry'CG
Cuts at: 104 4345 4391 5599 104
Size: 4241 46 1208 1308
Fragments arranged by size:
4241 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2198 2361 2470 3817 4595 4785 5577 2198
Size: 163 109 1347 778 190 792 3424
Fragments arranged by size:
3424 1347 792 778 190 163 109
BsmBI CGTCTCn'nnnn_
Cuts at: 1149 1236 2617 1149
Size: 87 1381 5335
Fragments arranged by size:
5335 1381 87
Bsp1286I G_dGCh'C
Cuts at: 1512 2198 2361 2470 3817 4428 4521 4595 4785
Size: 686 163 109 1347 611 93 74 190
Cuts at: 4785 4847 5577 1512
Size: 62 730 2738
Fragments arranged by size:
2738 1347 730 686 611 190 163 109
93 74 62
BspEI T'CCGG_A
Cuts at: 6023 6023
Size: 6803
BspHI T'CATG_A
Cuts at: 5979 5979
Size: 6803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3853 4067 4369 4750 3853
Size: 214 302 381 5906
Fragments arranged by size:
5906 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5095 5149 6633 6798 50
Size: 5045 54 1484 165 55
Fragments arranged by size:
5045 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 906 1079 1364 2501 4715 906
Size: 173 285 1137 2214 2994
Fragments arranged by size:
2994 2214 1137 285 173
BsrFI r'CCGG_y
Cuts at: 4801 4982 5191 4801
Size: 181 209 6413
Fragments arranged by size:
6413 209 181
BsrGI T'GTAC_A
Cuts at: 1329 3374 3565 1329
Size: 2045 191 4567
Fragments arranged by size:
4567 2045 191
BssSI C'ACGA_G
Cuts at: 5074 5432 5074
Size: 358 6445
BstAPI GCAn_nnn'nTGC
Cuts at: 487 1078 1206 2740 4180 487
Size: 591 128 1534 1440 3110
Fragments arranged by size:
3110 1534 1440 591 128
BstBI TT'CG_AA
Cuts at: 5164 5164
Size: 6803
BstEII G'GTnAC_C
Cuts at: 2018 2018
Size: 6803
BstXI CCAn_nnnn'nTGG
Cuts at: 141 228 141
Size: 87 6716
BstYI r'GATC_y
Cuts at: 1 744 1806 2302 3234 3828 4295 4318 4653
Size: 743 1062 496 932 594 467 23 335
Cuts at: 4653 4899 5911 5997 6009 6355 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1062 1012 932 743 594 496 467 449
346 335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 968 968
Size: 6803
BtgI C'CryG_G
Cuts at: 107 3010 107
Size: 2903 3900
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4753 4934 5097 4753
Size: 181 163 6459
Fragments arranged by size:
6459 181 163
BtsI GCAGTG_nn'
Cuts at: 683 4046 683
Size: 3363 3440
ClaI AT'CG_AT
Cuts at: 1592 1592
Size: 6803
DraI TTT'AAA
Cuts at: 1257 3321 3802 1257
Size: 2064 481 4258
Fragments arranged by size:
4258 2064 481
DrdI GACnn_nn'nnGTC
Cuts at: 4509 5367 4509
Size: 858 5945
EaeI y'GGCC_r
Cuts at: 705 2240 4388 4562 4953 4980 6325 705
Size: 1535 2148 174 391 27 1345 1183
Fragments arranged by size:
2148 1535 1345 1183 391 174 27
EagI C'GGCC_G
Cuts at: 4388 4388
Size: 6803
EarI CTCTTCn'nnn_
Cuts at: 1262 1427 3171 3673 4826 5036 1262
Size: 165 1744 502 1153 210 3029
Fragments arranged by size:
3029 1744 1153 502 210 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5011 5321 5467 6613 5011
Size: 310 146 1146 5201
Fragments arranged by size:
5201 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2196 2359 3815 2196
Size: 163 1456 5184
Fragments arranged by size:
5184 1456 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 446 1375 1658 1739 1927 4628 5060 5807 6075
Size: 929 283 81 188 2701 432 747 268
Cuts at: 6075 446
Size: 1174
Fragments arranged by size:
2701 1174 929 747 432 283 268 188
81
EcoO109I rG'GnC_Cy
Cuts at: 1508 1509 3942 1508
Size: 1 2433 4369
Fragments arranged by size:
4369 2433 1
EcoRI G'AATT_C
Cuts at: 1216 3697 3807 1216
Size: 2481 110 4212
Fragments arranged by size:
4212 2481 110
EcoRV GAT'ATC
Cuts at: 3032 3411 3032
Size: 379 6424
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 427 625 1744 3042 4822 427
Size: 198 1119 1298 1780 2408
Fragments arranged by size:
2408 1780 1298 1119 198
FspI TGC'GCA
Cuts at: 2437 4584 2437
Size: 2147 4656
HaeII r_GCGC'y
Cuts at: 119 4237 4485 5507 6486 119
Size: 4118 248 1022 979 436
Fragments arranged by size:
4118 1022 979 436 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 775 807 1218 1250 1585 1617 2294 2326 3395
Size: 32 411 32 335 32 677 32 1069
Cuts at: 3395 3427 3751 3783 4645 4675 4677 4707 775
Size: 32 324 32 862 30 2 30 2871
Fragments arranged by size:
2871 1069 862 677 411 335 324 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 659 794 1481 1985 2812 3842 659
Size: 135 687 504 827 1030 3620
Fragments arranged by size:
3620 1030 827 687 504 135
HindIII A'AGCT_T
Cuts at: 2696 2696
Size: 6803
HpaI GTT'AAC
Cuts at: 1481 1985 1481
Size: 504 6299
KasI G'GCGC_C
Cuts at: 4233 4481 4233
Size: 248 6555
KpnI G_GTAC'C
Cuts at: 3823 3823
Size: 6803
MfeI C'AATT_G
Cuts at: 365 365
Size: 6803
MluI A'CGCG_T
Cuts at: 6236 6236
Size: 6803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 209 1126 1671 2651 3512 5474 5658 209
Size: 917 545 980 861 1962 184 1354
Fragments arranged by size:
1962 1354 980 917 861 545 184
MscI TGG'CCA
Cuts at: 707 2242 4564 707
Size: 1535 2322 2946
Fragments arranged by size:
2946 2322 1535
MslI CAynn'nnrTG
Cuts at: 388 2758 3366 4792 4919 388
Size: 2370 608 1426 127 2272
Fragments arranged by size:
2370 2272 1426 608 127
MspA1I CmG'CkG
Cuts at: 1165 1843 2167 2554 2731 3914 4228 4588 5601
Size: 678 324 387 177 1183 314 360 1013
Cuts at: 5601 5846 1165
Size: 245 2122
Fragments arranged by size:
2122 1183 1013 678 387 360 324 314
245 177
NaeI GCC'GGC
Cuts at: 4984 4984
Size: 6803
NarI GG'CG_CC
Cuts at: 4234 4482 4234
Size: 248 6555
NcoI C'CATG_G
Cuts at: 107 107
Size: 6803
NdeI CA'TA_TG
Cuts at: 2889 3045 2889
Size: 156 6647
NgoMIV G'CCGG_C
Cuts at: 4982 4982
Size: 6803
NsiI A_TGCA'T
Cuts at: 2228 2745 2228
Size: 517 6286
NspI r_CATG'y
Cuts at: 393 1479 2230 2442 2537 3566 3856 4356 5263
Size: 1086 751 212 95 1029 290 500 907
Cuts at: 5263 393
Size: 1933
Fragments arranged by size:
1933 1086 1029 907 751 500 290 212
95
PciI A'CATG_T
Cuts at: 1475 3562 5259 1475
Size: 2087 1697 3019
Fragments arranged by size:
3019 2087 1697
PflMI CCAn_nnn'nTGG
Cuts at: 2391 2391
Size: 6803
PmeI GTTT'AAAC
Cuts at: 3802 3802
Size: 6803
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2045 2077 5942 5974 2045
Size: 32 3865 32 2874
Fragments arranged by size:
3865 2874 32 32
PshAI GACnn'nnGTC
Cuts at: 3392 3392
Size: 6803
PsiI TTA'TAA
Cuts at: 1000 1040 3295 1000
Size: 40 2255 4508
Fragments arranged by size:
4508 2255 40
PspOMI G'GGCC_C
Cuts at: 1508 1508
Size: 6803
PspXI vC'TCGA_Gb
Cuts at: 2528 2528
Size: 6803
PstI C_TGCA'G
Cuts at: 212 3527 3850 4535 212
Size: 3315 323 685 2480
Fragments arranged by size:
3315 2480 685 323
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6803
PvuII CAG'CTG
Cuts at: 1165 1843 2167 2554 2731 4228 4588 1165
Size: 678 324 387 177 1497 360 3380
Fragments arranged by size:
3380 1497 678 387 360 324 177
RsrII CG'GwC_CG
Cuts at: 4998 4998
Size: 6803
SacI G_AGCT'C
Cuts at: 2198 2361 3817 2198
Size: 163 1456 5184
Fragments arranged by size:
5184 1456 163
SalI G'TCGA_C
Cuts at: 3840 3840
Size: 6803
SapI GCTCTTCn'nnn_
Cuts at: 1427 4826 5036 1427
Size: 3399 210 3194
Fragments arranged by size:
3399 3194 210
SbfI CC_TGCA'GG
Cuts at: 3850 3850
Size: 6803
ScaI AGT'ACT
Cuts at: 928 3521 6320 928
Size: 2593 2799 1411
Fragments arranged by size:
2799 2593 1411
SexAI A'CCwGG_T
Cuts at: 1978 1978
Size: 6803
SfcI C'TryA_G
Cuts at: 33 208 1184 1988 2148 2555 2597 3523 3846
Size: 175 976 804 160 407 42 926 323
Cuts at: 3846 4531 5524 5715 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 976 926 804 685 407 323
191 175 160 42
SfoI GGC'GCC
Cuts at: 4235 4483 4235
Size: 248 6555
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6803
SmaI CCC'GGG
Cuts at: 3825 3825
Size: 6803
SmlI C'TyrA_G
Cuts at: 865 1637 2528 2589 2658 3016 3958 4873 5365
Size: 772 891 61 69 358 942 915 492
Cuts at: 5365 5627 865
Size: 262 2041
Fragments arranged by size:
2041 942 915 891 772 492 358 262
69 61
SnaBI TAC'GTA
Cuts at: 1614 1614
Size: 6803
SpeI A'CTAG_T
Cuts at: 67 2806 3793 67
Size: 2739 987 3077
Fragments arranged by size:
3077 2739 987
SphI G_CATG'C
Cuts at: 2442 2537 3856 4356 2442
Size: 95 1319 500 4889
Fragments arranged by size:
4889 1319 500 95
SspI AAT'ATT
Cuts at: 1005 1005
Size: 6803
StuI AGG'CCT
Cuts at: 2043 2043
Size: 6803
StyI C'CwwG_G
Cuts at: 107 569 1905 3185 3937 4291 107
Size: 462 1336 1280 752 354 2619
Fragments arranged by size:
2619 1336 1280 752 462 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3551 4386 4519 4978 5187 3551
Size: 835 133 459 209 5167
Fragments arranged by size:
5167 835 459 209 133
TatI w'GTAC_w
Cuts at: 926 1329 1530 3374 3519 3565 6318 926
Size: 403 201 1844 145 46 2753 1411
Fragments arranged by size:
2753 1844 1411 403 201 145 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6320 6352 6320
Size: 32 6771
Tth111I GACn'n_nGTC
Cuts at: 4600 4600
Size: 6803
XbaI T'CTAG_A
Cuts at: 2187 3384 3834 2187
Size: 1197 450 5156
Fragments arranged by size:
5156 1197 450
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4298 4298
Size: 6803
XhoI C'TCGA_G
Cuts at: 2528 2528
Size: 6803
XmaI C'CCGG_G
Cuts at: 3823 3823
Size: 6803
XmnI GAAnn'nnTTC
Cuts at: 410 1208 1724 2060 410
Size: 798 516 336 5153
Fragments arranged by size:
5153 798 516 336
ZraI GAC'GTC
Cuts at: 517 517
Size: 6803
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AscI AvrII BaeI BbvCI
BcgI BglI BmtI BsiWI BsmI BssHII BstZ17I CspCI
DraIII EcoNI FseI FspAI NheI NotI NruI PacI
PasI PfoI PmlI PpuMI PsrI SacII SanDI SfiI
SgrAI SrfI SwaI