Restriction map for
pF1KA0830
(Circular) (Six-base) MAPSORT of: pF1KA0830.seq Check: 9931 from: 1 to: 4613
pF1KA0830 4613 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1197 1197
Size: 4613
AccI GT'mk_AC
Cuts at: 1045 1651 1045
Size: 606 4007
Acc65I G'GTAC_C
Cuts at: 1522 1629 1522
Size: 107 4506
AclI AA'CG_TT
Cuts at: 4453 4453
Size: 4613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1189 1387 2438 2870 3617 3885 853
Size: 336 198 1051 432 747 268 1581
Fragments arranged by size:
1581 1051 747 432 336 268 198
AfeI AGC'GCT
Cuts at: 298 298
Size: 4613
AflIII A'CryG_T
Cuts at: 1965 3069 4046 1965
Size: 1104 977 2532
Fragments arranged by size:
2532 1104 977
AgeI A'CCGG_T
Cuts at: 3001 3001
Size: 4613
AleI CACnn'nnGTG
Cuts at: 466 466
Size: 4613
AlwNI CAG_nnn'CTG
Cuts at: 754 876 1918 3485 754
Size: 122 1042 1567 1882
Fragments arranged by size:
1882 1567 1042 122
ApaLI G'TGCA_C
Cuts at: 3383 3383
Size: 4613
ApoI r'AATT_y
Cuts at: 591 1617 3966 4222 591
Size: 1026 2349 256 982
Fragments arranged by size:
2349 1026 982 256
AseI AT'TA_AT
Cuts at: 20 443 1141 1214 20
Size: 423 698 73 3419
Fragments arranged by size:
3419 698 423 73
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
AvaI C'yCGr_G
Cuts at: 320 827 1633 320
Size: 507 806 3300
Fragments arranged by size:
3300 806 507
BamHI G'GATC_C
Cuts at: 1349 1638 1349
Size: 289 4324
BanI G'GyrC_C
Cuts at: 112 376 1522 1629 2043 2291 2326 112
Size: 264 1146 107 414 248 35 2399
Fragments arranged by size:
2399 1146 414 264 248 107 35
BanII G_rGCy'C
Cuts at: 1249 1341 1627 2657 1249
Size: 92 286 1030 3205
Fragments arranged by size:
3205 1030 286 92
BbeI G_GCGC'C
Cuts at: 380 2047 2295 380
Size: 1667 248 2698
Fragments arranged by size:
2698 1667 248
BbsI GAAGACnn'nnnn_
Cuts at: 695 695
Size: 4613
BbvCI CC'TCA_GC
Cuts at: 893 893
Size: 4613
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 126 160 126
Size: 34 4579
BciVI GTATCCnnnnn_n'
Cuts at: 2511 3272 4613 2511
Size: 761 1341 2511
Fragments arranged by size:
2511 1341 761
BclI T'GATC_A
Cuts at: 2133 2133
Size: 4613
BglI GCCn_nnn'nGGC
Cuts at: 243 1487 243
Size: 1244 3369
BglII A'GATC_T
Cuts at: 269 864 2128 4165 269
Size: 595 1264 2037 717
Fragments arranged by size:
2037 1264 717 595
BlpI GC'TnA_GC
Cuts at: 293 1248 1725 293
Size: 955 477 3181
Fragments arranged by size:
3181 955 477
Bme1580I G_kGCm'C
Cuts at: 115 719 2238 2331 3387 115
Size: 604 1519 93 1056 1341
Fragments arranged by size:
1519 1341 1056 604 93
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1825 1906 2241 4385 1486
Size: 339 81 335 2144 1714
Fragments arranged by size:
2144 1714 339 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1064 2980 1064
Size: 1916 2697
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 1043 812
Size: 231 4382
Bpu10I CC'TnA_GC
Cuts at: 553 893 553
Size: 340 4273
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 1297 1789 2668 3160 3458 451
Size: 846 492 879 492 298 1606
Fragments arranged by size:
1606 879 846 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4317 4317
Size: 4613
BsaAI yAC'GTr
Cuts at: 264 1966 264
Size: 1702 2911
BsaBI GATnn'nnATC
Cuts at: 6 2127 4164 6
Size: 2121 2037 455
Fragments arranged by size:
2121 2037 455
BsaHI Gr'CG_yC
Cuts at: 377 2044 2292 2994 4490 377
Size: 1667 248 702 1496 500
Fragments arranged by size:
1667 1496 702 500 248
BsaWI w'CCGG_w
Cuts at: 1346 1802 2323 3001 3275 3422 3833 1346
Size: 456 521 678 274 147 411 2126
Fragments arranged by size:
2126 678 521 456 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 532 562 532
Size: 30 4583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 448 809 1333 199
Size: 249 361 524 3479
Fragments arranged by size:
3479 524 361 249
BseYI C'CCAG_C
Cuts at: 132 154 800 2038 2796 3373 3869 132
Size: 22 646 1238 758 577 496 876
Fragments arranged by size:
1238 876 758 646 577 496 22
BsiEI CG_ry'CG
Cuts at: 104 254 2155 2201 3409 104
Size: 150 1901 46 1208 1308
Fragments arranged by size:
1901 1308 1208 150 46
BsiHKAI G_wGCw'C
Cuts at: 1313 1627 2405 2595 3387 1313
Size: 314 778 190 792 2539
Fragments arranged by size:
2539 792 778 314 190
BsmI GAATG_Cn'
Cuts at: 1295 1295
Size: 4613
Bsp1286I G_dGCh'C
Cuts at: 115 719 1249 1313 1341 1627 2238 2331 2405
Size: 604 530 64 28 286 611 93 74
Cuts at: 2405 2595 2657 3387 115
Size: 190 62 730 1341
Fragments arranged by size:
1341 730 611 604 530 286 190 93
74 64 62 28
BspEI T'CCGG_A
Cuts at: 1346 3833 1346
Size: 2487 2126
BspHI T'CATG_A
Cuts at: 3789 3789
Size: 4613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1197 1663 1877 2179 2560 1197
Size: 466 214 302 381 3250
Fragments arranged by size:
3250 466 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2905 2959 4443 4608 50
Size: 2855 54 1484 165 55
Fragments arranged by size:
2855 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1109 1469 2525 1109
Size: 360 1056 3197
Fragments arranged by size:
3197 1056 360
BsrFI r'CCGG_y
Cuts at: 170 193 2611 2792 3001 170
Size: 23 2418 181 209 1782
Fragments arranged by size:
2418 1782 209 181 23
BsrGI T'GTAC_A
Cuts at: 1433 1433
Size: 4613
BssHII G'CGCG_C
Cuts at: 118 279 118
Size: 161 4452
BssSI C'ACGA_G
Cuts at: 175 2884 3242 175
Size: 2709 358 1546
Fragments arranged by size:
2709 1546 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1990 1990
Size: 4613
BstBI TT'CG_AA
Cuts at: 2974 2974
Size: 4613
BstYI r'GATC_y
Cuts at: 1 269 864 1349 1638 2105 2128 2463 2709
Size: 268 595 485 289 467 23 335 246
Cuts at: 2709 3721 3807 3819 4165 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 595 485 467 449 346 335 289
268 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 806 1439 107
Size: 699 633 3281
Fragments arranged by size:
3281 699 633
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 402 2563 2744 2907 402
Size: 2161 181 163 2108
Fragments arranged by size:
2161 2108 181 163
BtsI GCAGTG_nn'
Cuts at: 564 687 1498 1511 1856 564
Size: 123 811 13 345 3321
Fragments arranged by size:
3321 811 345 123 13
ClaI AT'CG_AT
Cuts at: 410 410
Size: 4613
DraI TTT'AAA
Cuts at: 1612 1612
Size: 4613
DraIII CAC_nnn'GTG
Cuts at: 676 676
Size: 4613
DrdI GACnn_nn'nnGTC
Cuts at: 2319 3177 2319
Size: 858 3755
EaeI y'GGCC_r
Cuts at: 251 572 1271 1322 1418 2198 2372 2763 2790
Size: 321 699 51 96 780 174 391 27
Cuts at: 2790 4135 251
Size: 1345 729
Fragments arranged by size:
1345 780 729 699 391 321 174 96
51 27
EagI C'GGCC_G
Cuts at: 251 2198 251
Size: 1947 2666
EarI CTCTTCn'nnn_
Cuts at: 148 1277 2636 2846 148
Size: 1129 1359 210 1915
Fragments arranged by size:
1915 1359 1129 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2821 3131 3277 4423 2821
Size: 310 146 1146 3011
Fragments arranged by size:
3011 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1625 1625
Size: 4613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 812 853 1043 1189 1387 2438 2870 3617 3885
Size: 41 190 146 198 1051 432 747 268
Cuts at: 3885 812
Size: 1540
Fragments arranged by size:
1540 1051 747 432 268 198 190 146
41
EcoNI CCTnn'n_nnAGG
Cuts at: 433 794 1540 433
Size: 361 746 3506
Fragments arranged by size:
3506 746 361
EcoO109I rG'GnC_Cy
Cuts at: 230 1752 230
Size: 1522 3091
EcoRI G'AATT_C
Cuts at: 1617 1617
Size: 4613
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 510 2632 510
Size: 2122 2491
FspI TGC'GCA
Cuts at: 2394 2394
Size: 4613
HaeII r_GCGC'y
Cuts at: 300 380 1448 2047 2295 3317 4296 300
Size: 80 1068 599 248 1022 979 617
Fragments arranged by size:
1068 1022 979 617 599 248 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 999 1031 2455 2485 2487 2517 999
Size: 32 1424 30 2 30 3095
Fragments arranged by size:
3095 1424 32 30 30 2
HincII GTy'rAC
Cuts at: 966 1652 966
Size: 686 3927
HindIII A'AGCT_T
Cuts at: 1129 1129
Size: 4613
HpaI GTT'AAC
Cuts at: 966 966
Size: 4613
KasI G'GCGC_C
Cuts at: 376 2043 2291 376
Size: 1667 248 2698
Fragments arranged by size:
2698 1667 248
KpnI G_GTAC'C
Cuts at: 1526 1633 1526
Size: 107 4506
MfeI C'AATT_G
Cuts at: 1454 1454
Size: 4613
MluI A'CGCG_T
Cuts at: 4046 4046
Size: 4613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3284 3468 3284
Size: 184 4429
MscI TGG'CCA
Cuts at: 574 1273 1324 1420 2374 574
Size: 699 51 96 954 2813
Fragments arranged by size:
2813 954 699 96 51
MslI CAynn'nnrTG
Cuts at: 334 466 2602 2729 334
Size: 132 2136 127 2218
Fragments arranged by size:
2218 2136 132 127
MspA1I CmG'CkG
Cuts at: 897 1724 2038 2398 3411 3656 897
Size: 827 314 360 1013 245 1854
Fragments arranged by size:
1854 1013 827 360 314 245
NaeI GCC'GGC
Cuts at: 172 195 2794 172
Size: 23 2599 1991
Fragments arranged by size:
2599 1991 23
NarI GG'CG_CC
Cuts at: 377 2044 2292 377
Size: 1667 248 2698
Fragments arranged by size:
2698 1667 248
NcoI C'CATG_G
Cuts at: 107 806 1439 107
Size: 699 633 3281
Fragments arranged by size:
3281 699 633
NgoMIV G'CCGG_C
Cuts at: 170 193 2792 170
Size: 23 2599 1991
Fragments arranged by size:
2599 1991 23
NspI r_CATG'y
Cuts at: 1666 2166 3073 1666
Size: 500 907 3206
Fragments arranged by size:
3206 907 500
PasI CC'CwG_GG
Cuts at: 1527 1527
Size: 4613
PciI A'CATG_T
Cuts at: 3069 3069
Size: 4613
PflMI CCAn_nnn'nTGG
Cuts at: 676 676
Size: 4613
PfoI T'CCnGG_A
Cuts at: 617 953 974 617
Size: 336 21 4256
Fragments arranged by size:
4256 336 21
PmeI GTTT'AAAC
Cuts at: 1612 1612
Size: 4613
PmlI CAC'GTG
Cuts at: 264 264
Size: 4613
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4581
PpuMI rG'GwC_Cy
Cuts at: 230 230
Size: 4613
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 331 363 977 1009 331
Size: 32 614 32 3935
Fragments arranged by size:
3935 614 32 32
PstI C_TGCA'G
Cuts at: 1660 2345 1660
Size: 685 3928
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4613
PvuII CAG'CTG
Cuts at: 897 2038 2398 897
Size: 1141 360 3112
Fragments arranged by size:
3112 1141 360
RsrII CG'GwC_CG
Cuts at: 2808 2808
Size: 4613
SacI G_AGCT'C
Cuts at: 1627 1627
Size: 4613
SalI G'TCGA_C
Cuts at: 1650 1650
Size: 4613
SanDI GG'GwC_CC
Cuts at: 230 230
Size: 4613
SapI GCTCTTCn'nnn_
Cuts at: 2636 2846 2636
Size: 210 4403
SbfI CC_TGCA'GG
Cuts at: 1660 1660
Size: 4613
ScaI AGT'ACT
Cuts at: 4130 4130
Size: 4613
SfcI C'TryA_G
Cuts at: 33 312 726 1656 2341 3334 3525 33
Size: 279 414 930 685 993 191 1121
Fragments arranged by size:
1121 993 930 685 414 279 191
SfoI GGC'GCC
Cuts at: 378 2045 2293 378
Size: 1667 248 2698
Fragments arranged by size:
2698 1667 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4613
SmaI CCC'GGG
Cuts at: 322 829 1635 322
Size: 507 806 3300
Fragments arranged by size:
3300 806 507
SmlI C'TyrA_G
Cuts at: 430 1312 1768 2683 3175 3437 430
Size: 882 456 915 492 262 1606
Fragments arranged by size:
1606 915 882 492 456 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4613
SphI G_CATG'C
Cuts at: 1666 2166 1666
Size: 500 4113
StyI C'CwwG_G
Cuts at: 107 806 1439 1482 1747 2101 107
Size: 699 633 43 265 354 2619
Fragments arranged by size:
2619 699 633 354 265 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1519 2196 2329 2788 2997 1519
Size: 677 133 459 209 3135
Fragments arranged by size:
3135 677 459 209 133
TatI w'GTAC_w
Cuts at: 338 1433 4128 338
Size: 1095 2695 823
Fragments arranged by size:
2695 1095 823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4130 4162 4130
Size: 32 4581
Tth111I GACn'n_nGTC
Cuts at: 609 2410 609
Size: 1801 2812
XbaI T'CTAG_A
Cuts at: 1644 1644
Size: 4613
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2108 2108
Size: 4613
XmaI C'CCGG_G
Cuts at: 320 827 1633 320
Size: 507 806 3300
Fragments arranged by size:
3300 806 507
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BmgBI BmtI BsgI BsiWI BsmBI BstEII
BstXI BstZ17I Bsu36I CspCI EcoRV FseI FspAI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI