Restriction map for
pF1KA0840
(Circular) (Six-base) MAPSORT of: pF1KA0840.seq Check: 7291 from: 1 to: 4586
pF1KA0840 4586 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1624 1624
Size: 4586
Acc65I G'GTAC_C
Cuts at: 1166 1602 1166
Size: 436 4150
AclI AA'CG_TT
Cuts at: 4426 4426
Size: 4586
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2411 2843 3196 3858 2411
Size: 432 353 662 3139
Fragments arranged by size:
3139 662 432 353
AfeI AGC'GCT
Cuts at: 1546 1546
Size: 4586
AflIII A'CryG_T
Cuts at: 656 1938 3738 4019 656
Size: 1282 1800 281 1223
Fragments arranged by size:
1800 1282 1223 281
AhdI GACnn_n'nnGTC
Cuts at: 760 760
Size: 4586
AleI CACnn'nnGTG
Cuts at: 1201 1201
Size: 4586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1255 1255
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 638 692 1106 1482 1891 3329 638
Size: 54 414 376 409 1438 1895
Fragments arranged by size:
1895 1438 414 409 376 54
ApaLI G'TGCA_C
Cuts at: 370 3424 370
Size: 3054 1532
ApoI r'AATT_y
Cuts at: 1590 3939 4195 1590
Size: 2349 256 1981
Fragments arranged by size:
2349 1981 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 156 878 1606 156
Size: 722 728 3136
Fragments arranged by size:
3136 728 722
BamHI G'GATC_C
Cuts at: 1611 1611
Size: 4586
BanI G'GyrC_C
Cuts at: 1166 1602 2016 2264 2299 1166
Size: 436 414 248 35 3453
Fragments arranged by size:
3453 436 414 248 35
BanII G_rGCy'C
Cuts at: 325 1600 2630 325
Size: 1275 1030 2281
Fragments arranged by size:
2281 1275 1030
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 239 239
Size: 4586
BciVI GTATCCnnnnn_n'
Cuts at: 1362 2484 3540 4586 1362
Size: 1122 1056 1046 1362
Fragments arranged by size:
1362 1122 1056 1046
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BglI GCCn_nnn'nGGC
Cuts at: 587 587
Size: 4586
BglII A'GATC_T
Cuts at: 479 2101 4138 479
Size: 1622 2037 927
Fragments arranged by size:
2037 1622 927
BlpI GC'TnA_GC
Cuts at: 1093 1405 1698 1093
Size: 312 293 3981
Fragments arranged by size:
3981 312 293
Bme1580I G_kGCm'C
Cuts at: 374 2211 2304 3428 374
Size: 1837 93 1124 1532
Fragments arranged by size:
1837 1532 1124 93
BmgBI CAC'GTC
Cuts at: 174 1203 174
Size: 1029 3557
BmrI ACTGGGnnnn_n'
Cuts at: 726 1798 1879 2214 4358 726
Size: 1072 81 335 2144 954
Fragments arranged by size:
2144 1072 954 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 588 847 982 1171 1387 1459 405
Size: 183 259 135 189 216 72 3532
Fragments arranged by size:
3532 259 216 189 183 135 72
Bpu10I CC'TnA_GC
Cuts at: 244 1171 244
Size: 927 3659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 601 1381 1396 1762 2641 3355 3653 601
Size: 780 15 366 879 714 298 1534
Fragments arranged by size:
1534 879 780 714 366 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 586 1509 4290 586
Size: 923 2781 882
Fragments arranged by size:
2781 923 882
BsaAI yAC'GTr
Cuts at: 1218 1939 1218
Size: 721 3865
BsaBI GATnn'nnATC
Cuts at: 6 925 2100 4137 6
Size: 919 1175 2037 455
Fragments arranged by size:
2037 1175 919 455
BsaHI Gr'CG_yC
Cuts at: 2017 2265 2967 4463 2017
Size: 248 702 1496 2140
Fragments arranged by size:
2140 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3385 3532 3806 1775
Size: 521 678 411 147 274 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 281 311 766 796 1063 1093 281
Size: 30 455 30 267 30 3774
Fragments arranged by size:
3774 455 267 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 324
Size: 4586
BseYI C'CCAG_C
Cuts at: 268 273 2011 2769 3434 3842 268
Size: 5 1738 758 665 408 1012
Fragments arranged by size:
1738 1012 758 665 408 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 961 981 1293 496
Size: 465 20 312 3789
Fragments arranged by size:
3789 465 312 20
BsiEI CG_ry'CG
Cuts at: 104 1191 2128 2174 3404 104
Size: 1087 937 46 1230 1286
Fragments arranged by size:
1286 1230 1087 937 46
BsiHKAI G_wGCw'C
Cuts at: 265 374 833 1562 1600 2378 2568 3428 265
Size: 109 459 729 38 778 190 860 1423
Fragments arranged by size:
1423 860 778 729 459 190 109 38
BsmBI CGTCTCn'nnnn_
Cuts at: 349 349
Size: 4586
Bsp1286I G_dGCh'C
Cuts at: 265 325 374 833 1562 1600 2211 2304 2378
Size: 60 49 459 729 38 611 93 74
Cuts at: 2378 2568 2630 3428 265
Size: 190 62 798 1423
Fragments arranged by size:
1423 798 729 611 459 190 93 74
62 60 49 38
BspHI T'CATG_A
Cuts at: 3018 3018
Size: 4586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 871 1028 1636 1850 2152 2533 871
Size: 157 608 214 302 381 2924
Fragments arranged by size:
2924 608 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 405 2878 2932 4416 4581 50
Size: 355 2473 54 1484 165 55
Fragments arranged by size:
2473 1484 355 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2498 2498
Size: 4586
BsrFI r'CCGG_y
Cuts at: 452 1432 2584 2765 452
Size: 980 1152 181 2273
Fragments arranged by size:
2273 1152 980 181
BsrGI T'GTAC_A
Cuts at: 719 719
Size: 4586
BssHII G'CGCG_C
Cuts at: 518 518
Size: 4586
BssSI C'ACGA_G
Cuts at: 2857 3565 2857
Size: 708 3878
BstAPI GCAn_nnn'nTGC
Cuts at: 638 1547 1963 638
Size: 909 416 3261
Fragments arranged by size:
3261 909 416
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstEII G'GTnAC_C
Cuts at: 1194 1194
Size: 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 603 603
Size: 4586
BstYI r'GATC_y
Cuts at: 1 479 1611 2078 2101 2436 2682 2988 3000
Size: 478 1132 467 23 335 246 306 12
Cuts at: 3000 3086 4138 1
Size: 86 1052 449
Fragments arranged by size:
1132 1052 478 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 365 470 559 1281 1508 107
Size: 258 105 89 722 227 3185
Fragments arranged by size:
3185 722 258 227 105 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 711 969 1161 2536 2717 2880 711
Size: 258 192 1375 181 163 2417
Fragments arranged by size:
2417 1375 258 192 181 163
BtsI GCAGTG_nn'
Cuts at: 144 993 1182 1536 1829 144
Size: 849 189 354 293 2901
Fragments arranged by size:
2901 849 354 293 189
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DraIII CAC_nnn'GTG
Cuts at: 1287 1287
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 1501 2292 3636 1501
Size: 791 1344 2451
Fragments arranged by size:
2451 1344 791
EaeI y'GGCC_r
Cuts at: 362 1188 1220 1434 1454 2171 2345 2736 2763
Size: 826 32 214 20 717 174 391 27
Cuts at: 2763 4108 362
Size: 1345 840
Fragments arranged by size:
1345 840 826 717 391 214 174 32
27 20
EagI C'GGCC_G
Cuts at: 1188 2171 1188
Size: 983 3603
EarI CTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 568 1024 2794 3536 3682 4396 568
Size: 456 1770 742 146 714 758
Fragments arranged by size:
1770 758 742 714 456 146
EcoICRI GAG'CTC
Cuts at: 1598 1598
Size: 4586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 588 847 982 1171 1387 1459 2411 2843
Size: 183 259 135 189 216 72 952 432
Cuts at: 2843 3196 3858 405
Size: 353 662 1133
Fragments arranged by size:
1133 952 662 432 353 259 216 189
183 135 72
EcoO109I rG'GnC_Cy
Cuts at: 555 1515 1725 555
Size: 960 210 3416
Fragments arranged by size:
3416 960 210
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
EcoRV GAT'ATC
Cuts at: 1332 1332
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4586
FspI TGC'GCA
Cuts at: 253 2367 253
Size: 2114 2472
FspAI rTGC'GCAy
Cuts at: 253 253
Size: 4586
HaeII r_GCGC'y
Cuts at: 1548 2020 2268 3498 4269 1548
Size: 472 248 1230 771 1865
Fragments arranged by size:
1865 1230 771 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 746 778 793 825 2428 2458 2460 2490 746
Size: 32 15 32 1603 30 2 30 2842
Fragments arranged by size:
2842 1603 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 1625 1625
Size: 4586
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1170 1606 1170
Size: 436 4150
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 263 809 1379 3345 3529 263
Size: 546 570 1966 184 1320
Fragments arranged by size:
1966 1320 570 546 184
MscI TGG'CCA
Cuts at: 364 1222 2347 364
Size: 858 1125 2603
Fragments arranged by size:
2603 1125 858
MslI CAynn'nnrTG
Cuts at: 601 836 1201 2575 2702 601
Size: 235 365 1374 127 2485
Fragments arranged by size:
2485 1374 365 235 127
MspA1I CmG'CkG
Cuts at: 507 515 536 561 1028 1697 2011 2371 3155
Size: 8 21 25 467 669 314 360 784
Cuts at: 3155 3400 507
Size: 245 1693
Fragments arranged by size:
1693 784 669 467 360 314 245 25
21 8
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NcoI C'CATG_G
Cuts at: 107 365 470 107
Size: 258 105 4223
Fragments arranged by size:
4223 258 105
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NspI r_CATG'y
Cuts at: 252 1639 2139 3742 252
Size: 1387 500 1603 1096
Fragments arranged by size:
1603 1387 1096 500
PciI A'CATG_T
Cuts at: 3738 3738
Size: 4586
PflMI CCAn_nnn'nTGG
Cuts at: 471 1287 471
Size: 816 3770
PfoI T'CCnGG_A
Cuts at: 458 1490 458
Size: 1032 3554
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3032 3064 3032
Size: 32 4554
PpuMI rG'GwC_Cy
Cuts at: 555 555
Size: 4586
PspXI vC'TCGA_Gb
Cuts at: 156 156
Size: 4586
PstI C_TGCA'G
Cuts at: 697 1234 1633 2318 697
Size: 537 399 685 2965
Fragments arranged by size:
2965 685 537 399
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 507 2011 2371 507
Size: 1504 360 2722
Fragments arranged by size:
2722 1504 360
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 1600 1600
Size: 4586
SacII CC_GC'GG
Cuts at: 562 562
Size: 4586
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SanDI GG'GwC_CC
Cuts at: 555 555
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 2609 2819 2609
Size: 210 4376
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 1228 4103 1228
Size: 2875 1711
SexAI A'CCwGG_T
Cuts at: 1061 1061
Size: 4586
SfcI C'TryA_G
Cuts at: 33 693 1230 1629 2314 3282 3473 33
Size: 660 537 399 685 968 191 1146
Fragments arranged by size:
1146 968 685 660 537 399 191
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 880 1608 880
Size: 728 3858
SmlI C'TyrA_G
Cuts at: 156 616 1396 1411 1741 2656 3370 3632 156
Size: 460 780 15 330 915 714 262 1110
Fragments arranged by size:
1110 915 780 714 460 330 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 252 1639 2139 252
Size: 1387 500 2699
Fragments arranged by size:
2699 1387 500
StuI AGG'CCT
Cuts at: 886 974 1052 886
Size: 88 78 4420
Fragments arranged by size:
4420 88 78
StyI C'CwwG_G
Cuts at: 107 365 470 1720 2074 107
Size: 258 105 1250 354 2619
Fragments arranged by size:
2619 1250 354 258 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 725 1024 1057 2169 2302 2761 2970 725
Size: 299 33 1112 133 459 209 2341
Fragments arranged by size:
2341 1112 459 299 209 133 33
TatI w'GTAC_w
Cuts at: 184 719 1226 4101 184
Size: 535 507 2875 669
Fragments arranged by size:
2875 669 535 507
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 281 313 4103 4135 281
Size: 32 3790 32 732
Fragments arranged by size:
3790 732 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 933 2081 933
Size: 1148 3438
XhoI C'TCGA_G
Cuts at: 156 156
Size: 4586
XmaI C'CCGG_G
Cuts at: 878 1606 878
Size: 728 3858
XmnI GAAnn'nnTTC
Cuts at: 1387 1387
Size: 4586
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsiWI BsmI BspEI
BstZ17I Bsu36I ClaI CspCI EcoNI FseI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PsrI SfiI SgrAI SnaBI
SrfI SspI SwaI ZraI