Restriction map for
pF1KA0847
(Circular) (Six-base) MAPSORT of: pF1KA0847.seq Check: 1676 from: 1 to: 6845
pF1KA0847 6845 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 638 638
Size: 6845
AccI GT'mk_AC
Cuts at: 474 3883 474
Size: 3409 3436
Acc65I G'GTAC_C
Cuts at: 3861 3861
Size: 6845
AclI AA'CG_TT
Cuts at: 6685 6685
Size: 6845
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 1334 1774 1877 1979 2132 2176 2287 2459
Size: 1060 440 103 102 153 44 111 172
Cuts at: 2459 2645 3236 4670 5102 5455 6117 274
Size: 186 591 1434 432 353 662 1002
Fragments arranged by size:
1434 1060 1002 662 591 440 432 353
186 172 153 111 103 102 44
AflIII A'CryG_T
Cuts at: 571 749 2327 2691 4197 5997 6278 571
Size: 178 1578 364 1506 1800 281 1138
Fragments arranged by size:
1800 1578 1506 1138 364 281 178
AhdI GACnn_n'nnGTC
Cuts at: 735 735
Size: 6845
AlwNI CAG_nnn'CTG
Cuts at: 1495 2995 4150 5588 1495
Size: 1500 1155 1438 2752
Fragments arranged by size:
2752 1500 1438 1155
ApaLI G'TGCA_C
Cuts at: 5683 5683
Size: 6845
ApoI r'AATT_y
Cuts at: 209 868 1295 2413 2666 3263 3446 3849 6198
Size: 659 427 1118 253 597 183 403 2349
Cuts at: 6198 6454 209
Size: 256 600
Fragments arranged by size:
2349 1118 659 600 597 427 403 256
253 183
AseI AT'TA_AT
Cuts at: 20 20
Size: 6845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6845
AvaI C'yCGr_G
Cuts at: 434 647 3865 434
Size: 213 3218 3414
Fragments arranged by size:
3414 3218 213
AvrII C'CTAG_G
Cuts at: 3399 3399
Size: 6845
BamHI G'GATC_C
Cuts at: 3760 3870 3760
Size: 110 6735
BanI G'GyrC_C
Cuts at: 2607 2884 3861 4275 4523 4558 2607
Size: 277 977 414 248 35 4894
Fragments arranged by size:
4894 977 414 277 248 35
BanII G_rGCy'C
Cuts at: 1732 2207 3859 4889 1732
Size: 475 1652 1030 3688
Fragments arranged by size:
3688 1652 1030 475
BbeI G_GCGC'C
Cuts at: 4279 4527 4279
Size: 248 6597
BbsI GAAGACnn'nnnn_
Cuts at: 726 1009 1339 2774 726
Size: 283 330 1435 4797
Fragments arranged by size:
4797 1435 330 283
BciVI GTATCCnnnnn_n'
Cuts at: 4743 5799 6845 4743
Size: 1056 1046 4743
Fragments arranged by size:
4743 1056 1046
BclI T'GATC_A
Cuts at: 1926 2388 4365 1926
Size: 462 1977 4406
Fragments arranged by size:
4406 1977 462
BfrBI ATG'CAT
Cuts at: 685 685
Size: 6845
BglII A'GATC_T
Cuts at: 414 2187 2510 4360 6397 414
Size: 1773 323 1850 2037 862
Fragments arranged by size:
2037 1850 1773 862 323
BlpI GC'TnA_GC
Cuts at: 1162 3957 1162
Size: 2795 4050
Bme1580I G_kGCm'C
Cuts at: 2889 4470 4563 5687 2889
Size: 1581 93 1124 4047
Fragments arranged by size:
4047 1581 1124 93
BmgBI CAC'GTC
Cuts at: 3596 3596
Size: 6845
BmrI ACTGGGnnnn_n'
Cuts at: 1005 3240 3554 4057 4138 4473 6617 1005
Size: 2235 314 503 81 335 2144 1233
Fragments arranged by size:
2235 2144 1233 503 335 314 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2532 2982 3500 5212 2532
Size: 450 518 1712 4165
Fragments arranged by size:
4165 1712 518 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 848 1133 1498 1540 1885 1894 2008 2051
Size: 44 285 365 42 345 9 114 43
Cuts at: 2051 2972 3386 3690 804
Size: 921 414 304 3959
Fragments arranged by size:
3959 921 414 365 345 304 285 114
44 43 42 9
Bpu10I CC'TnA_GC
Cuts at: 1679 1726 1679
Size: 47 6798
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1528 1871 2345 3078 4021 4900 5614 5912 1528
Size: 343 474 733 943 879 714 298 2461
Fragments arranged by size:
2461 943 879 733 714 474 343 298
BsaI GGTCTCn'nnnn_
Cuts at: 1504 2783 6549 1504
Size: 1279 3766 1800
Fragments arranged by size:
3766 1800 1279
BsaAI yAC'GTr
Cuts at: 4198 4198
Size: 6845
BsaBI GATnn'nnATC
Cuts at: 6 4359 6396 6
Size: 4353 2037 455
Fragments arranged by size:
4353 2037 455
BsaHI Gr'CG_yC
Cuts at: 635 2059 4276 4524 5226 6722 635
Size: 1424 2217 248 702 1496 758
Fragments arranged by size:
2217 1496 1424 758 702 248
BsaWI w'CCGG_w
Cuts at: 4034 4555 5233 5644 5791 6065 4034
Size: 521 678 411 147 274 4814
Fragments arranged by size:
4814 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2519 2549 3415 3445 3546 3576 2519
Size: 30 866 30 101 30 5788
Fragments arranged by size:
5788 866 101 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 1333 1864 2505 3001 3133 3169 3497 3560
Size: 1199 531 641 496 132 36 328 63
Cuts at: 3560 3657 3693 134
Size: 97 36 3286
Fragments arranged by size:
3286 1199 641 531 496 328 132 97
63 36 36
BseYI C'CCAG_C
Cuts at: 468 3682 3739 4270 5028 5693 6101 468
Size: 3214 57 531 758 665 408 1212
Fragments arranged by size:
3214 1212 758 665 531 408 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1565 1565
Size: 6845
BsiEI CG_ry'CG
Cuts at: 104 4387 4433 5663 104
Size: 4283 46 1230 1286
Fragments arranged by size:
4283 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1732 3167 3481 3859 4637 4827 5687 1732
Size: 1435 314 378 778 190 860 2890
Fragments arranged by size:
2890 1435 860 778 378 314 190
BsmI GAATG_Cn'
Cuts at: 500 500
Size: 6845
BsmBI CGTCTCn'nnnn_
Cuts at: 3323 3323
Size: 6845
Bsp1286I G_dGCh'C
Cuts at: 1732 2207 2889 3167 3481 3859 4470 4563 4637
Size: 475 682 278 314 378 611 93 74
Cuts at: 4637 4827 4889 5687 1732
Size: 190 62 798 2890
Fragments arranged by size:
2890 798 682 611 475 378 314 278
190 93 74 62
BspHI T'CATG_A
Cuts at: 2322 2759 5277 2322
Size: 437 2518 3890
Fragments arranged by size:
3890 2518 437
BspMI ACCTGCnnnn'nnnn_
Cuts at: 649 1522 3895 4109 4411 4792 649
Size: 873 2373 214 302 381 2702
Fragments arranged by size:
2702 2373 873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1388 5137 5191 6675 6840 50
Size: 1338 3749 54 1484 165 55
Fragments arranged by size:
3749 1484 1338 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1710 3729 4757 1710
Size: 2019 1028 3798
Fragments arranged by size:
3798 2019 1028
BsrFI r'CCGG_y
Cuts at: 4843 5024 4843
Size: 181 6664
BssSI C'ACGA_G
Cuts at: 3357 5116 5824 3357
Size: 1759 708 4378
Fragments arranged by size:
4378 1759 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1969 4222 1969
Size: 2253 4592
BstBI TT'CG_AA
Cuts at: 5206 5206
Size: 6845
BstEII G'GTnAC_C
Cuts at: 1370 1370
Size: 6845
BstXI CCAn_nnnn'nTGG
Cuts at: 1342 3281 1342
Size: 1939 4906
BstYI r'GATC_y
Cuts at: 1 414 1216 2187 2510 3460 3502 3694 3760
Size: 413 802 971 323 950 42 192 66
Cuts at: 3760 3870 4337 4360 4695 4941 5247 5259 5345
Size: 110 467 23 335 246 306 12 86
Cuts at: 5345 6397 1
Size: 1052 449
Fragments arranged by size:
1052 971 950 802 467 449 413 335
323 306 246 192 110 86 66 42
23 12
Bsu36I CC'TnA_GG
Cuts at: 1947 1947
Size: 6845
BtgI C'CryG_G
Cuts at: 1329 2198 1329
Size: 869 5976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1181 4795 4976 5139 1181
Size: 3614 181 163 2887
Fragments arranged by size:
3614 2887 181 163
BtsI GCAGTG_nn'
Cuts at: 4088 4088
Size: 6845
ClaI AT'CG_AT
Cuts at: 369 369
Size: 6845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 609 644 609
Size: 35 6810
DraI TTT'AAA
Cuts at: 1934 3844 1934
Size: 1910 4935
DrdI GACnn_nn'nnGTC
Cuts at: 1232 4551 5895 1232
Size: 3319 1344 2182
Fragments arranged by size:
3319 2182 1344
EaeI y'GGCC_r
Cuts at: 1332 3334 4430 4604 4995 5022 6367 1332
Size: 2002 1096 174 391 27 1345 1810
Fragments arranged by size:
2002 1810 1345 1096 391 174 27
EagI C'GGCC_G
Cuts at: 4430 4430
Size: 6845
EarI CTCTTCn'nnn_
Cuts at: 900 1253 1310 2435 3805 4868 5078 900
Size: 353 57 1125 1370 1063 210 2667
Fragments arranged by size:
2667 1370 1125 1063 353 210 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5053 5795 5941 6655 5053
Size: 742 146 714 5243
Fragments arranged by size:
5243 742 714 146
EcoICRI GAG'CTC
Cuts at: 1730 3857 1730
Size: 2127 4718
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 804 848 1133 1334 1498 1540 1774 1877
Size: 530 44 285 201 164 42 234 103
Cuts at: 1877 1885 1894 1979 2008 2051 2132 2176 2287
Size: 8 9 85 29 43 81 44 111
Cuts at: 2287 2459 2645 2972 3236 3386 3690 4670 5102
Size: 172 186 327 264 150 304 980 432
Cuts at: 5102 5455 6117 274
Size: 353 662 1002
Fragments arranged by size:
1002 980 662 530 432 353 327 304
285 264 234 201 186 172 164 150
111 103 85 81 44 44 43 42
29 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2276 3705 3708 2276
Size: 1429 3 5413
Fragments arranged by size:
5413 1429 3
EcoO109I rG'GnC_Cy
Cuts at: 1785 1909 1978 2092 3713 3984 1785
Size: 124 69 114 1621 271 4646
Fragments arranged by size:
4646 1621 271 124 114 69
EcoRI G'AATT_C
Cuts at: 868 3849 868
Size: 2981 3864
EcoRV GAT'ATC
Cuts at: 135 2741 135
Size: 2606 4239
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1657 2113 4864 1657
Size: 456 2751 3638
Fragments arranged by size:
3638 2751 456
FspI TGC'GCA
Cuts at: 4626 4626
Size: 6845
HaeII r_GCGC'y
Cuts at: 4279 4527 5757 6528 4279
Size: 248 1230 771 4596
Fragments arranged by size:
4596 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 721 753 877 909 2502 2534 2816
Size: 32 338 32 124 32 1593 32 282
Cuts at: 2816 2848 3380 3412 3415 3447 4687 4717 4719
Size: 32 532 32 3 32 1240 30 2
Cuts at: 4719 4749 351
Size: 30 2447
Fragments arranged by size:
2447 1593 1240 532 338 282 124 32
32 32 32 32 32 32 30 30
3 2
HincII GTy'rAC
Cuts at: 1722 2144 3288 3884 1722
Size: 422 1144 596 4683
Fragments arranged by size:
4683 1144 596 422
HindIII A'AGCT_T
Cuts at: 1288 2154 2641 3046 1288
Size: 866 487 405 5087
Fragments arranged by size:
5087 866 487 405
HpaI GTT'AAC
Cuts at: 1722 1722
Size: 6845
KasI G'GCGC_C
Cuts at: 4275 4523 4275
Size: 248 6597
KpnI G_GTAC'C
Cuts at: 3865 3865
Size: 6845
MfeI C'AATT_G
Cuts at: 1082 1082
Size: 6845
MluI A'CGCG_T
Cuts at: 6278 6278
Size: 6845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 126 382 2634 3440 5604 5788 126
Size: 256 2252 806 2164 184 1183
Fragments arranged by size:
2252 2164 1183 806 256 184
MscI TGG'CCA
Cuts at: 1334 4606 1334
Size: 3272 3573
MslI CAynn'nnrTG
Cuts at: 754 1628 4834 4961 754
Size: 874 3206 127 2638
Fragments arranged by size:
3206 2638 874 127
MspA1I CmG'CkG
Cuts at: 777 3214 3956 4270 4630 5414 5659 777
Size: 2437 742 314 360 784 245 1963
Fragments arranged by size:
2437 1963 784 742 360 314 245
NaeI GCC'GGC
Cuts at: 5026 5026
Size: 6845
NarI GG'CG_CC
Cuts at: 4276 4524 4276
Size: 248 6597
NcoI C'CATG_G
Cuts at: 1329 2198 1329
Size: 869 5976
NgoMIV G'CCGG_C
Cuts at: 5024 5024
Size: 6845
NsiI A_TGCA'T
Cuts at: 687 687
Size: 6845
NspI r_CATG'y
Cuts at: 231 575 591 753 2331 2695 3898 4398 6001
Size: 344 16 162 1578 364 1203 500 1603
Cuts at: 6001 231
Size: 1075
Fragments arranged by size:
1603 1578 1203 1075 500 364 344 162
16
PasI CC'CwG_GG
Cuts at: 2428 2428
Size: 6845
PciI A'CATG_T
Cuts at: 571 749 2327 2691 5997 571
Size: 178 1578 364 3306 1419
Fragments arranged by size:
3306 1578 1419 364 178
PflMI CCAn_nnn'nTGG
Cuts at: 1366 1760 1366
Size: 394 6451
PfoI T'CCnGG_A
Cuts at: 1871 3498 3706 1871
Size: 1627 208 5010
Fragments arranged by size:
5010 1627 208
PmeI GTTT'AAAC
Cuts at: 3844 3844
Size: 6845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 659 691 5291 5323 659
Size: 32 4600 32 2181
Fragments arranged by size:
4600 2181 32 32
PpuMI rG'GwC_Cy
Cuts at: 1785 1909 1978 2092 3713 1785
Size: 124 69 114 1621 4917
Fragments arranged by size:
4917 1621 124 114 69
PspXI vC'TCGA_Gb
Cuts at: 647 647
Size: 6845
PstI C_TGCA'G
Cuts at: 1974 3892 4577 1974
Size: 1918 685 4242
Fragments arranged by size:
4242 1918 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6845
PvuII CAG'CTG
Cuts at: 777 3214 4270 4630 777
Size: 2437 1056 360 2992
Fragments arranged by size:
2992 2437 1056 360
RsrII CG'GwC_CG
Cuts at: 5040 5040
Size: 6845
SacI G_AGCT'C
Cuts at: 1732 3859 1732
Size: 2127 4718
SalI G'TCGA_C
Cuts at: 3882 3882
Size: 6845
SapI GCTCTTCn'nnn_
Cuts at: 4868 5078 4868
Size: 210 6635
SbfI CC_TGCA'GG
Cuts at: 3892 3892
Size: 6845
ScaI AGT'ACT
Cuts at: 1862 6362 1862
Size: 4500 2345
SfcI C'TryA_G
Cuts at: 33 1814 1970 3888 4573 5541 5732 33
Size: 1781 156 1918 685 968 191 1146
Fragments arranged by size:
1918 1781 1146 968 685 191 156
SfoI GGC'GCC
Cuts at: 4277 4525 4277
Size: 248 6597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6845
SmaI CCC'GGG
Cuts at: 3867 3867
Size: 6845
SmlI C'TyrA_G
Cuts at: 647 1507 1886 2360 3093 4000 4915 5629 5891
Size: 860 379 474 733 907 915 714 262
Cuts at: 5891 647
Size: 1601
Fragments arranged by size:
1601 915 907 860 733 714 474 379
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6845
SphI G_CATG'C
Cuts at: 3898 4398 3898
Size: 500 6345
StuI AGG'CCT
Cuts at: 3363 3363
Size: 6845
StyI C'CwwG_G
Cuts at: 221 1329 1365 1912 2198 2227 3081 3268 3399
Size: 1108 36 547 286 29 854 187 131
Cuts at: 3399 3804 3979 4333 221
Size: 405 175 354 2733
Fragments arranged by size:
2733 1108 854 547 405 354 286 187
175 131 36 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1752 3249 3369 3543 4428 4561 5020 5229 1752
Size: 1497 120 174 885 133 459 209 3368
Fragments arranged by size:
3368 1497 885 459 209 174 133 120
TatI w'GTAC_w
Cuts at: 568 1860 6360 568
Size: 1292 4500 1053
Fragments arranged by size:
4500 1292 1053
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 140 172 3546 3578 6362 6394 140
Size: 32 3374 32 2784 32 591
Fragments arranged by size:
3374 2784 591 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3040 4642 3040
Size: 1602 5243
XbaI T'CTAG_A
Cuts at: 882 3250 3876 882
Size: 2368 626 3851
Fragments arranged by size:
3851 2368 626
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 2070 4340 465
Size: 1605 2270 2970
Fragments arranged by size:
2970 2270 1605
XhoI C'TCGA_G
Cuts at: 647 647
Size: 6845
XmaI C'CCGG_G
Cuts at: 3865 3865
Size: 6845
XmnI GAAnn'nnTTC
Cuts at: 500 1559 1640 1839 1862 2855 500
Size: 1059 81 199 23 993 4490
Fragments arranged by size:
4490 1059 993 199 81 23
ZraI GAC'GTC
Cuts at: 636 636
Size: 6845
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BglI BmtI BsiWI BspEI
BsrGI BssHII BstZ17I DraIII FseI FspAI NdeI NheI
NotI NruI PacI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI