Restriction map for
pF1KA0850
(Circular) (Six-base) MAPSORT of: pF1KA0850.seq Check: 3458 from: 1 to: 5039
pF1KA0850 5039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1216 1216
Size: 5039
AccI GT'mk_AC
Cuts at: 2077 2077
Size: 5039
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 4879 4879
Size: 5039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 463 623 971 2864 3296 4043 4311 199
Size: 264 160 348 1893 432 747 268 927
Fragments arranged by size:
1893 927 747 432 348 268 264 160
AflII C'TTAA_G
Cuts at: 619 619
Size: 5039
AflIII A'CryG_T
Cuts at: 2391 3495 4472 2391
Size: 1104 977 2958
Fragments arranged by size:
2958 1104 977
AgeI A'CCGG_T
Cuts at: 3427 3427
Size: 5039
AhdI GACnn_n'nnGTC
Cuts at: 217 217
Size: 5039
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1757 1789 1757
Size: 32 5007
AlwNI CAG_nnn'CTG
Cuts at: 352 2344 3911 352
Size: 1992 1567 1480
Fragments arranged by size:
1992 1567 1480
ApaLI G'TGCA_C
Cuts at: 3809 3809
Size: 5039
ApoI r'AATT_y
Cuts at: 144 314 328 522 596 1960 2043 4392 4648
Size: 170 14 194 74 1364 83 2349 256
Cuts at: 4648 144
Size: 535
Fragments arranged by size:
2349 1364 535 256 194 170 83 74
14
AseI AT'TA_AT
Cuts at: 20 20
Size: 5039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 2059 2059
Size: 5039
BamHI G'GATC_C
Cuts at: 2064 2064
Size: 5039
BanI G'GyrC_C
Cuts at: 2055 2469 2717 2752 2055
Size: 414 248 35 4342
Fragments arranged by size:
4342 414 248 35
BanII G_rGCy'C
Cuts at: 1084 1326 2011 2053 3083 1084
Size: 242 685 42 1030 3040
Fragments arranged by size:
3040 1030 685 242 42
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbsI GAAGACnn'nnnn_
Cuts at: 149 1243 149
Size: 1094 3945
BbvCI CC'TCA_GC
Cuts at: 830 830
Size: 5039
BciVI GTATCCnnnnn_n'
Cuts at: 2937 3698 5039 2937
Size: 761 1341 2937
Fragments arranged by size:
2937 1341 761
BclI T'GATC_A
Cuts at: 789 2559 789
Size: 1770 3269
BglII A'GATC_T
Cuts at: 2554 4591 2554
Size: 2037 3002
BlpI GC'TnA_GC
Cuts at: 2151 2151
Size: 5039
Bme1580I G_kGCm'C
Cuts at: 1596 2664 2757 3813 1596
Size: 1068 93 1056 2822
Fragments arranged by size:
2822 1068 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 717 2251 2332 2667 4811 717
Size: 1534 81 335 2144 945
Fragments arranged by size:
2144 1534 945 335 81
BmtI G_CTAG'C
Cuts at: 259 259
Size: 5039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 1784
Size: 5039
Bpu10I CC'TnA_GC
Cuts at: 830 962 830
Size: 132 4907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2011 2215 3094 3586 3884 2011
Size: 204 879 492 298 3166
Fragments arranged by size:
3166 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 4743 4743
Size: 5039
BsaAI yAC'GTr
Cuts at: 886 2392 886
Size: 1506 3533
BsaBI GATnn'nnATC
Cuts at: 6 302 341 2553 4590 6
Size: 296 39 2212 2037 455
Fragments arranged by size:
2212 2037 455 296 39
BsaHI Gr'CG_yC
Cuts at: 2470 2718 3420 4916 2470
Size: 248 702 1496 2593
Fragments arranged by size:
2593 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2228 2749 3427 3701 3848 4259 2228
Size: 521 678 274 147 411 3008
Fragments arranged by size:
3008 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 574 604 1080 1110 1751 1757 1781 1787 574
Size: 30 476 30 641 6 24 6 3826
Fragments arranged by size:
3826 641 476 30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 622 625 1772 622
Size: 3 1147 3889
Fragments arranged by size:
3889 1147 3
BseYI C'CCAG_C
Cuts at: 673 1903 2464 3222 3799 4295 673
Size: 1230 561 758 577 496 1417
Fragments arranged by size:
1417 1230 758 577 561 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 889 1685 733
Size: 156 796 4087
Fragments arranged by size:
4087 796 156
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3835 104
Size: 2477 46 1208 1308
Fragments arranged by size:
2477 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1084 1488 2053 2831 3021 3813 1084
Size: 404 565 778 190 792 2310
Fragments arranged by size:
2310 792 778 565 404 190
BsmI GAATG_Cn'
Cuts at: 1270 1270
Size: 5039
Bsp1286I G_dGCh'C
Cuts at: 1084 1326 1488 1596 2011 2053 2664 2757 2831
Size: 242 162 108 415 42 611 93 74
Cuts at: 2831 3021 3083 3813 1084
Size: 190 62 730 2310
Fragments arranged by size:
2310 730 611 415 242 190 162 108
93 74 62 42
BspEI T'CCGG_A
Cuts at: 4259 4259
Size: 5039
BspHI T'CATG_A
Cuts at: 4215 4215
Size: 5039
BspMI ACCTGCnnnn'nnnn_
Cuts at: 453 1216 2089 2303 2605 2986 453
Size: 763 873 214 302 381 2506
Fragments arranged by size:
2506 873 763 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3331 3385 4869 5034 50
Size: 3281 54 1484 165 55
Fragments arranged by size:
3281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 684 1905 1962 2951 684
Size: 1221 57 989 2772
Fragments arranged by size:
2772 1221 989 57
BsrFI r'CCGG_y
Cuts at: 3037 3218 3427 3037
Size: 181 209 4649
Fragments arranged by size:
4649 209 181
BsrGI T'GTAC_A
Cuts at: 1649 1649
Size: 5039
BssSI C'ACGA_G
Cuts at: 3310 3668 3310
Size: 358 4681
BstAPI GCAn_nnn'nTGC
Cuts at: 2416 2416
Size: 5039
BstBI TT'CG_AA
Cuts at: 1254 3400 1254
Size: 2146 2893
BstXI CCAn_nnnn'nTGG
Cuts at: 1746 1746
Size: 5039
BstYI r'GATC_y
Cuts at: 1 2064 2531 2554 2889 3135 4147 4233 4245
Size: 2063 467 23 335 246 1012 86 12
Cuts at: 4245 4591 1
Size: 346 449
Fragments arranged by size:
2063 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 181 181
Size: 5039
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 257 380 849 1943 2282 257
Size: 123 469 1094 339 3014
Fragments arranged by size:
3014 1094 469 339 123
DraI TTT'AAA
Cuts at: 2038 2038
Size: 5039
DraIII CAC_nnn'GTG
Cuts at: 886 886
Size: 5039
DrdI GACnn_nn'nnGTC
Cuts at: 2745 3603 2745
Size: 858 4181
EaeI y'GGCC_r
Cuts at: 192 225 894 1380 2624 2798 3189 3216 4561
Size: 33 669 486 1244 174 391 27 1345
Cuts at: 4561 192
Size: 670
Fragments arranged by size:
1345 1244 670 669 486 391 174 33
27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 599 743 755 939 3062 3272 599
Size: 144 12 184 2123 210 2366
Fragments arranged by size:
2366 2123 210 184 144 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3247 3557 3703 4849 3247
Size: 310 146 1146 3437
Fragments arranged by size:
3437 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 2051 1082
Size: 969 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 463 623 971 1784 2864 3296 4043 4311
Size: 264 160 348 813 1080 432 747 268
Cuts at: 4311 199
Size: 927
Fragments arranged by size:
1080 927 813 747 432 348 268 264
160
EcoO109I rG'GnC_Cy
Cuts at: 2178 2178
Size: 5039
EcoRI G'AATT_C
Cuts at: 2043 2043
Size: 5039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5039
FspI TGC'GCA
Cuts at: 2820 2820
Size: 5039
HaeII r_GCGC'y
Cuts at: 2473 2721 3743 4722 2473
Size: 248 1022 979 2790
Fragments arranged by size:
2790 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 203 235 1276 1308 2881 2911 2913 2943 203
Size: 32 1041 32 1573 30 2 30 2299
Fragments arranged by size:
2299 1573 1041 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2078 2078
Size: 5039
HindIII A'AGCT_T
Cuts at: 622 1119 622
Size: 497 4542
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 620 1873 3710 3894 620
Size: 1253 1837 184 1765
Fragments arranged by size:
1837 1765 1253 184
MscI TGG'CCA
Cuts at: 194 227 896 1382 2800 194
Size: 33 669 486 1418 2433
Fragments arranged by size:
2433 1418 669 486 33
MslI CAynn'nnrTG
Cuts at: 1388 1744 3028 3155 1388
Size: 356 1284 127 3272
Fragments arranged by size:
3272 1284 356 127
MspA1I CmG'CkG
Cuts at: 506 787 2150 2464 2824 3837 4082 506
Size: 281 1363 314 360 1013 245 1463
Fragments arranged by size:
1463 1363 1013 360 314 281 245
NaeI GCC'GGC
Cuts at: 3220 3220
Size: 5039
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NdeI CA'TA_TG
Cuts at: 1527 1527
Size: 5039
NgoMIV G'CCGG_C
Cuts at: 3218 3218
Size: 5039
NheI G'CTAG_C
Cuts at: 255 255
Size: 5039
NspI r_CATG'y
Cuts at: 2092 2592 3499 2092
Size: 500 907 3632
Fragments arranged by size:
3632 907 500
PciI A'CATG_T
Cuts at: 3495 3495
Size: 5039
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PmlI CAC'GTG
Cuts at: 886 886
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1757 1789 4178 4210 1757
Size: 32 2389 32 2586
Fragments arranged by size:
2586 2389 32 32
PshAI GACnn'nnGTC
Cuts at: 1617 1617
Size: 5039
PstI C_TGCA'G
Cuts at: 268 432 1227 1626 2086 2771 268
Size: 164 795 399 460 685 2536
Fragments arranged by size:
2536 795 685 460 399 164
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 506 787 2464 2824 506
Size: 281 1677 360 2721
Fragments arranged by size:
2721 1677 360 281
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 1084 2053 1084
Size: 969 4070
SalI G'TCGA_C
Cuts at: 2076 2076
Size: 5039
SapI GCTCTTCn'nnn_
Cuts at: 743 3062 3272 743
Size: 2319 210 2510
Fragments arranged by size:
2510 2319 210
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 1095 4556 1095
Size: 3461 1578
SfcI C'TryA_G
Cuts at: 33 264 428 947 1223 1622 1916 2082 2767
Size: 231 164 519 276 399 294 166 685
Cuts at: 2767 3760 3951 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 519 399 294 276 231
191 166 164
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 619 1990 2194 3109 3601 3863 619
Size: 1371 204 915 492 262 1795
Fragments arranged by size:
1795 1371 915 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5039
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
StyI C'CwwG_G
Cuts at: 628 1891 2173 2527 628
Size: 1263 282 354 3140
Fragments arranged by size:
3140 1263 354 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 699 1752 2622 2755 3214 3423 699
Size: 1053 870 133 459 209 2315
Fragments arranged by size:
2315 1053 870 459 209 133
TatI w'GTAC_w
Cuts at: 776 951 1093 1341 1649 4554 776
Size: 175 142 248 308 2905 1261
Fragments arranged by size:
2905 1261 308 248 175 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1575 1607 1751 1783 4556 4588 1575
Size: 32 144 32 2773 32 2026
Fragments arranged by size:
2773 2026 144 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 487 2070 487
Size: 1583 3456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1830 1935 2534 1830
Size: 105 599 4335
Fragments arranged by size:
4335 599 105
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
XmnI GAAnn'nnTTC
Cuts at: 764 1250 764
Size: 486 4553
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NheI NspI PciI PmeI PmlI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AleI AlfI ApaI AscI AvrII BaeI
BcgI BfrBI BglI BmgBI BsiWI BsmBI BssHII BstEII
BstZ17I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HpaI MfeI NcoI NotI NruI NsiI PacI PasI
PflMI PfoI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI