Restriction map for
pF1KA0853
(Circular) (Six-base) MAPSORT of: pF1KA0853.seq Check: 508 from: 1 to: 8117
pF1KA0853 8117 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4519 4519
Size: 8117
AccI GT'mk_AC
Cuts at: 1054 2102 4982 5155 1054
Size: 1048 2880 173 4016
Fragments arranged by size:
4016 2880 1048 173
Acc65I G'GTAC_C
Cuts at: 5133 5133
Size: 8117
AclI AA'CG_TT
Cuts at: 7957 7957
Size: 8117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 776 2957 3086 3884 4881 5081 5942 6374
Size: 511 2181 129 798 997 200 861 432
Cuts at: 6374 6727 7389 265
Size: 353 662 993
Fragments arranged by size:
2181 997 993 861 798 662 511 432
353 200 129
AfeI AGC'GCT
Cuts at: 2581 2581
Size: 8117
AflIII A'CryG_T
Cuts at: 274 4750 5469 7269 7550 274
Size: 4476 719 1800 281 841
Fragments arranged by size:
4476 1800 841 719 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2793 2825 2793
Size: 32 8085
AlwNI CAG_nnn'CTG
Cuts at: 3305 4723 5100 5422 6860 3305
Size: 1418 377 322 1438 4562
Fragments arranged by size:
4562 1438 1418 377 322
ApaLI G'TGCA_C
Cuts at: 2906 6955 2906
Size: 4049 4068
ApoI r'AATT_y
Cuts at: 855 2990 4520 4666 5121 7470 7726 855
Size: 2135 1530 146 455 2349 256 1246
Fragments arranged by size:
2349 2135 1530 1246 455 256 146
AseI AT'TA_AT
Cuts at: 20 575 637 20
Size: 555 62 7500
Fragments arranged by size:
7500 555 62
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8117
AvaI C'yCGr_G
Cuts at: 1463 3682 4325 5137 1463
Size: 2219 643 812 4443
Fragments arranged by size:
4443 2219 812 643
BamHI G'GATC_C
Cuts at: 5142 5142
Size: 8117
BanI G'GyrC_C
Cuts at: 4945 5133 5547 5795 5830 4945
Size: 188 414 248 35 7232
Fragments arranged by size:
7232 414 248 188 35
BanII G_rGCy'C
Cuts at: 407 1432 2964 4815 5131 6161 407
Size: 1025 1532 1851 316 1030 2363
Fragments arranged by size:
2363 1851 1532 1030 1025 316
BbeI G_GCGC'C
Cuts at: 5551 5799 5551
Size: 248 7869
BbsI GAAGACnn'nnnn_
Cuts at: 180 253 488 782 820 1986 2486 2762 3575
Size: 73 235 294 38 1166 500 276 813
Cuts at: 3575 3715 4849 4999 5032 180
Size: 140 1134 150 33 3265
Fragments arranged by size:
3265 1166 1134 813 500 294 276 235
150 140 73 38 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3663 3697 3663
Size: 34 8083
BciVI GTATCCnnnnn_n'
Cuts at: 1659 4480 5098 6015 7071 8117 1659
Size: 2821 618 917 1056 1046 1659
Fragments arranged by size:
2821 1659 1056 1046 917 618
BclI T'GATC_A
Cuts at: 1311 3861 5637 1311
Size: 2550 1776 3791
Fragments arranged by size:
3791 2550 1776
BglII A'GATC_T
Cuts at: 3973 4365 5632 7669 3973
Size: 392 1267 2037 4421
Fragments arranged by size:
4421 2037 1267 392
BlpI GC'TnA_GC
Cuts at: 5229 5229
Size: 8117
Bme1580I G_kGCm'C
Cuts at: 2910 4948 5742 5835 6959 2910
Size: 2038 794 93 1124 4068
Fragments arranged by size:
4068 2038 1124 794 93
BmgBI CAC'GTC
Cuts at: 393 393
Size: 8117
BmrI ACTGGGnnnn_n'
Cuts at: 173 3878 5329 5410 5745 7889 173
Size: 3705 1451 81 335 2144 401
Fragments arranged by size:
3705 2144 1451 401 335 81
BmtI G_CTAG'C
Cuts at: 800 800
Size: 8117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 450 6484 450
Size: 6034 2083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 1509 3929 4499 4697 5027 494
Size: 1015 2420 570 198 330 3584
Fragments arranged by size:
3584 2420 1015 570 330 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1380 1527 1824 2089 2702 2718 2821 2880 3786
Size: 147 297 265 613 16 103 59 906
Cuts at: 3786 5293 6172 6886 7184 1380
Size: 1507 879 714 298 2313
Fragments arranged by size:
2313 1507 906 879 714 613 298 297
265 147 103 59 16
BsaI GGTCTCn'nnnn_
Cuts at: 1433 1988 2288 2294 7821 1433
Size: 555 300 6 5527 1729
Fragments arranged by size:
5527 1729 555 300 6
BsaAI yAC'GTr
Cuts at: 892 3624 5470 892
Size: 2732 1846 3539
Fragments arranged by size:
3539 2732 1846
BsaBI GATnn'nnATC
Cuts at: 6 1652 1670 1928 2540 4364 5631 7668 6
Size: 1646 18 258 612 1824 1267 2037 455
Fragments arranged by size:
2037 1824 1646 1267 612 455 258 18
BsaHI Gr'CG_yC
Cuts at: 1344 3616 5548 5796 6498 7994 1344
Size: 2272 1932 248 702 1496 1467
Fragments arranged by size:
2272 1932 1496 1467 702 248
BsaWI w'CCGG_w
Cuts at: 2643 5306 5827 6505 6916 7063 7337 2643
Size: 2663 521 678 411 147 274 3423
Fragments arranged by size:
3423 2663 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1182 1212 3375 3405 3687 3717 4956 4986 1182
Size: 30 2163 30 282 30 1239 30 4313
Fragments arranged by size:
4313 2163 1239 282 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 439 1142 2794 3383 3404 3684 4065 4579 439
Size: 703 1652 589 21 280 381 514 3977
Fragments arranged by size:
3977 1652 703 589 514 381 280 21
BseYI C'CCAG_C
Cuts at: 202 1062 1892 2602 3173 5542 6300 6965 7373
Size: 860 830 710 571 2369 758 665 408
Cuts at: 7373 202
Size: 946
Fragments arranged by size:
2369 946 860 830 758 710 665 571
408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4399 4640 3775
Size: 624 241 7252
Fragments arranged by size:
7252 624 241
BsiEI CG_ry'CG
Cuts at: 104 2310 5659 5705 6935 104
Size: 2206 3349 46 1230 1286
Fragments arranged by size:
3349 2206 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1432 2910 2964 4974 5131 5909 6099 6959 1432
Size: 1478 54 2010 157 778 190 860 2590
Fragments arranged by size:
2590 2010 1478 860 778 190 157 54
BsiWI C'GTAC_G
Cuts at: 1726 1726
Size: 8117
BsmI GAATG_Cn'
Cuts at: 2906 3295 2906
Size: 389 7728
BsmBI CGTCTCn'nnnn_
Cuts at: 1253 3607 1253
Size: 2354 5763
Bsp1286I G_dGCh'C
Cuts at: 407 1432 2910 2964 4815 4948 4974 5131 5742
Size: 1025 1478 54 1851 133 26 157 611
Cuts at: 5742 5835 5909 6099 6161 6959 407
Size: 93 74 190 62 798 1565
Fragments arranged by size:
1851 1565 1478 1025 798 611 190 157
133 93 74 62 54 26
BspEI T'CCGG_A
Cuts at: 2643 2643
Size: 8117
BspHI T'CATG_A
Cuts at: 591 1314 1335 1884 2964 6549 591
Size: 723 21 549 1080 3585 2159
Fragments arranged by size:
3585 2159 1080 723 549 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4519 4708 5167 5381 5683 6064 4519
Size: 189 459 214 302 381 6572
Fragments arranged by size:
6572 459 381 302 214 189
BsrBI CCG'CTC
Cuts at: 50 2172 2316 6409 6463 7947 8112 50
Size: 2122 144 4093 54 1484 165 55
Fragments arranged by size:
4093 2122 1484 165 144 55 54
BsrDI GCAATG_nn'
Cuts at: 3252 6029 3252
Size: 2777 5340
BsrFI r'CCGG_y
Cuts at: 2627 6115 6296 2627
Size: 3488 181 4448
Fragments arranged by size:
4448 3488 181
BssSI C'ACGA_G
Cuts at: 302 1610 3596 4640 4965 6388 7096 302
Size: 1308 1986 1044 325 1423 708 1323
Fragments arranged by size:
1986 1423 1323 1308 1044 708 325
BstAPI GCAn_nnn'nTGC
Cuts at: 3463 4534 5494 3463
Size: 1071 960 6086
Fragments arranged by size:
6086 1071 960
BstBI TT'CG_AA
Cuts at: 4917 6478 4917
Size: 1561 6556
BstEII G'GTnAC_C
Cuts at: 335 335
Size: 8117
BstXI CCAn_nnnn'nTGG
Cuts at: 3757 3757
Size: 8117
BstYI r'GATC_y
Cuts at: 1 357 2525 3973 4308 4365 4372 4578 5142
Size: 356 2168 1448 335 57 7 206 564
Cuts at: 5142 5609 5632 5967 6213 6519 6531 6617 7669
Size: 467 23 335 246 306 12 86 1052
Cuts at: 7669 1
Size: 449
Fragments arranged by size:
2168 1448 1052 564 467 449 356 335
335 306 246 206 86 57 23 12
7
BtgI C'CryG_G
Cuts at: 3199 3199
Size: 8117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1264 3631 6067 6248 6411 1264
Size: 2367 2436 181 163 2970
Fragments arranged by size:
2970 2436 2367 181 163
BtsI GCAGTG_nn'
Cuts at: 3437 5360 3437
Size: 1923 6194
ClaI AT'CG_AT
Cuts at: 4017 4017
Size: 8117
DraI TTT'AAA
Cuts at: 5116 5116
Size: 8117
DraIII CAC_nnn'GTG
Cuts at: 304 304
Size: 8117
DrdI GACnn_nn'nnGTC
Cuts at: 5823 7167 5823
Size: 1344 6773
EaeI y'GGCC_r
Cuts at: 1695 4099 5702 5876 6267 6294 7639 1695
Size: 2404 1603 174 391 27 1345 2173
Fragments arranged by size:
2404 2173 1603 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5702 5702
Size: 8117
EarI CTCTTCn'nnn_
Cuts at: 661 1437 2079 2091 2363 2516 3410 6140 6350
Size: 776 642 12 272 153 894 2730 210
Cuts at: 6350 661
Size: 2428
Fragments arranged by size:
2730 2428 894 776 642 272 210 153
12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6325 7067 7213 7927 6325
Size: 742 146 714 6515
Fragments arranged by size:
6515 742 714 146
EcoICRI GAG'CTC
Cuts at: 1430 2962 5129 1430
Size: 1532 2167 4418
Fragments arranged by size:
4418 2167 1532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 494 776 1509 2957 3086 3884 3929 4499
Size: 229 282 733 1448 129 798 45 570
Cuts at: 4499 4697 4881 5027 5081 5942 6374 6727 7389
Size: 198 184 146 54 861 432 353 662
Cuts at: 7389 265
Size: 993
Fragments arranged by size:
1448 993 861 798 733 662 570 432
353 282 229 198 184 146 129 54
45
EcoO109I rG'GnC_Cy
Cuts at: 5256 5256
Size: 8117
EcoRI G'AATT_C
Cuts at: 5121 5121
Size: 8117
EcoRV GAT'ATC
Cuts at: 927 1930 927
Size: 1003 7114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1073 1187 1982 3914 6136 1073
Size: 114 795 1932 2222 3054
Fragments arranged by size:
3054 2222 1932 795 114
FspI TGC'GCA
Cuts at: 5898 5898
Size: 8117
HaeII r_GCGC'y
Cuts at: 387 2583 2628 2820 5551 5799 7029 7800 387
Size: 2196 45 192 2731 248 1230 771 704
Fragments arranged by size:
2731 2196 1230 771 704 248 192 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 415 447 1182 1214 1296 1328 1414
Size: 32 232 32 735 32 82 32 86
Cuts at: 1414 1446 1572 1588 1604 1620 2510 2542 2940
Size: 32 126 16 16 16 890 32 398
Cuts at: 2940 2972 3046 3078 3835 3867 4357 4389 4423
Size: 32 74 32 757 32 490 32 34
Cuts at: 4423 4455 4563 4595 5959 5989 5991 6021 151
Size: 32 108 32 1364 30 2 30 2247
Fragments arranged by size:
2247 1364 890 757 735 490 398 232
126 108 86 82 74 34 32 32
32 32 32 32 32 32 32 32
32 32 30 30 16 16 16 2
HincII GTy'rAC
Cuts at: 5156 5156
Size: 8117
HindIII A'AGCT_T
Cuts at: 4958 4958
Size: 8117
KasI G'GCGC_C
Cuts at: 5547 5795 5547
Size: 248 7869
KpnI G_GTAC'C
Cuts at: 5137 5137
Size: 8117
MluI A'CGCG_T
Cuts at: 7550 7550
Size: 8117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 821 3012 5115 6876 7060 821
Size: 2191 2103 1761 184 1878
Fragments arranged by size:
2191 2103 1878 1761 184
MscI TGG'CCA
Cuts at: 4101 5878 4101
Size: 1777 6340
MslI CAynn'nnrTG
Cuts at: 1313 1448 3755 6106 6233 1313
Size: 135 2307 2351 127 3197
Fragments arranged by size:
3197 2351 2307 135 127
MspA1I CmG'CkG
Cuts at: 1188 1892 5228 5542 5902 6686 6931 1188
Size: 704 3336 314 360 784 245 2374
Fragments arranged by size:
3336 2374 784 704 360 314 245
NaeI GCC'GGC
Cuts at: 2629 6298 2629
Size: 3669 4448
NarI GG'CG_CC
Cuts at: 5548 5796 5548
Size: 248 7869
NgoMIV G'CCGG_C
Cuts at: 2627 6296 2627
Size: 3669 4448
NheI G'CTAG_C
Cuts at: 796 796
Size: 8117
NspI r_CATG'y
Cuts at: 278 1536 4754 5170 5670 7273 278
Size: 1258 3218 416 500 1603 1122
Fragments arranged by size:
3218 1603 1258 1122 500 416
PciI A'CATG_T
Cuts at: 274 4750 7269 274
Size: 4476 2519 1122
Fragments arranged by size:
4476 2519 1122
PflMI CCAn_nnn'nTGG
Cuts at: 393 2551 393
Size: 2158 5959
PfoI T'CCnGG_A
Cuts at: 1684 2885 5043 1684
Size: 1201 2158 4758
Fragments arranged by size:
4758 2158 1201
PmeI GTTT'AAAC
Cuts at: 5116 5116
Size: 8117
PmlI CAC'GTG
Cuts at: 3624 3624
Size: 8117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 6563 6595 2819
Size: 32 3712 32 4341
Fragments arranged by size:
4341 3712 32 32
PstI C_TGCA'G
Cuts at: 825 5164 5849 825
Size: 4339 685 3093
Fragments arranged by size:
4339 3093 685
PvuI CG_AT'CG
Cuts at: 104 2310 104
Size: 2206 5911
PvuII CAG'CTG
Cuts at: 1892 5542 5902 1892
Size: 3650 360 4107
Fragments arranged by size:
4107 3650 360
RsrII CG'GwC_CG
Cuts at: 6312 6312
Size: 8117
SacI G_AGCT'C
Cuts at: 1432 2964 5131 1432
Size: 1532 2167 4418
Fragments arranged by size:
4418 2167 1532
SalI G'TCGA_C
Cuts at: 5154 5154
Size: 8117
SapI GCTCTTCn'nnn_
Cuts at: 1437 6140 6350 1437
Size: 4703 210 3204
Fragments arranged by size:
4703 3204 210
SbfI CC_TGCA'GG
Cuts at: 5164 5164
Size: 8117
ScaI AGT'ACT
Cuts at: 3788 3894 7634 3788
Size: 106 3740 4271
Fragments arranged by size:
4271 3740 106
SfcI C'TryA_G
Cuts at: 33 350 821 1626 2583 2855 3629 5160 5845
Size: 317 471 805 957 272 774 1531 685
Cuts at: 5845 6813 7004 33
Size: 968 191 1146
Fragments arranged by size:
1531 1146 968 957 805 774 685 471
317 272 191
SfoI GGC'GCC
Cuts at: 5549 5797 5549
Size: 248 7869
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8117
SgrAI Cr'CCGG_yG
Cuts at: 2627 2627
Size: 8117
SmaI CCC'GGG
Cuts at: 5139 5139
Size: 8117
SmlI C'TyrA_G
Cuts at: 1395 1463 1542 1839 2068 2697 2717 2836 2895
Size: 68 79 297 229 629 20 119 59
Cuts at: 2895 3801 5272 6187 6901 7163 1395
Size: 906 1471 915 714 262 2349
Fragments arranged by size:
2349 1471 915 906 714 629 297 262
229 119 79 68 59 20
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8117
SphI G_CATG'C
Cuts at: 5170 5670 5170
Size: 500 7617
StyI C'CwwG_G
Cuts at: 1048 4340 5251 5605 1048
Size: 3292 911 354 3560
Fragments arranged by size:
3560 3292 911 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1520 1592 1796 2312 4094 5700 5833 6292 6501
Size: 72 204 516 1782 1606 133 459 209
Cuts at: 6501 1520
Size: 3136
Fragments arranged by size:
3136 1782 1606 516 459 209 204 133
72
TatI w'GTAC_w
Cuts at: 214 3786 3892 7632 214
Size: 3572 106 3740 699
Fragments arranged by size:
3740 3572 699 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1093 1125 4956 4988 7634 7666 1093
Size: 32 3831 32 2646 32 1544
Fragments arranged by size:
3831 2646 1544 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5914 5914
Size: 8117
XbaI T'CTAG_A
Cuts at: 1093 1435 3264 4311 5148 1093
Size: 342 1829 1047 837 4062
Fragments arranged by size:
4062 1829 1047 837 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3973 5612 3973
Size: 1639 6478
XhoI C'TCGA_G
Cuts at: 1463 1463
Size: 8117
XmaI C'CCGG_G
Cuts at: 5137 5137
Size: 8117
XmnI GAAnn'nnTTC
Cuts at: 1170 3894 3939 4994 1170
Size: 2724 45 1055 4293
Fragments arranged by size:
4293 2724 1055 45
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AgeI AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI Bpu10I BsrGI BssHII
BstZ17I Bsu36I CspCI EcoNI FseI FspAI HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SnaBI SrfI SspI StuI SwaI ZraI