Restriction map for
pF1KA0864
(Circular) (Six-base) MAPSORT of: pF1KA0864.seq Check: 3551 from: 1 to: 6227
pF1KA0864 6227 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2055 2055
Size: 6227
AccI GT'mk_AC
Cuts at: 3265 3265
Size: 6227
Acc65I G'GTAC_C
Cuts at: 3243 3243
Size: 6227
AclI AA'CG_TT
Cuts at: 6067 6067
Size: 6227
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 965 2117 3178 4052 4484 4837 5499 965
Size: 1152 1061 874 432 353 662 1693
Fragments arranged by size:
1693 1152 1061 874 662 432 353
AfeI AGC'GCT
Cuts at: 1891 1891
Size: 6227
AflIII A'CryG_T
Cuts at: 3579 5379 5660 3579
Size: 1800 281 4146
Fragments arranged by size:
4146 1800 281
AgeI A'CCGG_T
Cuts at: 299 299
Size: 6227
AhdI GACnn_n'nnGTC
Cuts at: 1233 1233
Size: 6227
AleI CACnn'nnGTG
Cuts at: 421 421
Size: 6227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2555 2587 2555
Size: 32 6195
AlwNI CAG_nnn'CTG
Cuts at: 957 1375 1770 1927 2535 2628 2649 2700 2853
Size: 418 395 157 608 93 21 51 153
Cuts at: 2853 3116 3532 4970 957
Size: 263 416 1438 2214
Fragments arranged by size:
2214 1438 608 418 416 395 263 157
153 93 51 21
ApaI G_GGCC'C
Cuts at: 1082 1082
Size: 6227
ApaLI G'TGCA_C
Cuts at: 295 414 1552 1862 5065 295
Size: 119 1138 310 3203 1457
Fragments arranged by size:
3203 1457 1138 310 119
ApoI r'AATT_y
Cuts at: 139 1269 3231 5580 5836 139
Size: 1130 1962 2349 256 530
Fragments arranged by size:
2349 1962 1130 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6227
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
AvaI C'yCGr_G
Cuts at: 1632 2143 3247 1632
Size: 511 1104 4612
Fragments arranged by size:
4612 1104 511
AvrII C'CTAG_G
Cuts at: 1132 1132
Size: 6227
BamHI G'GATC_C
Cuts at: 1529 3252 1529
Size: 1723 4504
BanI G'GyrC_C
Cuts at: 394 2067 2525 3243 3657 3905 3940 394
Size: 1673 458 718 414 248 35 2681
Fragments arranged by size:
2681 1673 718 458 414 248 35
BanII G_rGCy'C
Cuts at: 599 1082 1247 1820 1966 2681 2951 3241 4271
Size: 483 165 573 146 715 270 290 1030
Cuts at: 4271 599
Size: 2555
Fragments arranged by size:
2555 1030 715 573 483 290 270 165
146
BbeI G_GCGC'C
Cuts at: 2529 3661 3909 2529
Size: 1132 248 4847
Fragments arranged by size:
4847 1132 248
BbsI GAAGACnn'nnnn_
Cuts at: 928 2084 928
Size: 1156 5071
BbvCI CC'TCA_GC
Cuts at: 1376 2218 1376
Size: 842 5385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 286 320 1875 1909 286
Size: 34 1555 34 4604
Fragments arranged by size:
4604 1555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1448 4125 5181 6227 1448
Size: 2677 1056 1046 1448
Fragments arranged by size:
2677 1448 1056 1046
BclI T'GATC_A
Cuts at: 3747 3747
Size: 6227
BglI GCCn_nnn'nGGC
Cuts at: 112 446 631 2617 112
Size: 334 185 1986 3722
Fragments arranged by size:
3722 1986 334 185
BglII A'GATC_T
Cuts at: 2328 3742 5779 2328
Size: 1414 2037 2776
Fragments arranged by size:
2776 2037 1414
BlpI GC'TnA_GC
Cuts at: 2950 3339 2950
Size: 389 5838
Bme1580I G_kGCm'C
Cuts at: 299 397 418 1082 1556 1866 2072 3852 3945
Size: 98 21 664 474 310 206 1780 93
Cuts at: 3945 5069 299
Size: 1124 1457
Fragments arranged by size:
1780 1457 1124 664 474 310 206 98
93 21
BmrI ACTGGGnnnn_n'
Cuts at: 286 674 1749 2759 2783 3034 3439 3520 3855
Size: 388 1075 1010 24 251 405 81 335
Cuts at: 3855 5999 286
Size: 2144 514
Fragments arranged by size:
2144 1075 1010 514 405 388 335 251
81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 788 812 1740 2151 2592 2604 2886 4594 788
Size: 24 928 411 441 12 282 1708 2421
Fragments arranged by size:
2421 1708 928 441 411 282 24 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 553 937 1870 2176 2209 2317 2488 2557
Size: 302 384 933 306 33 108 171 69
Cuts at: 2557 2587 2623 2650 251
Size: 30 36 27 3828
Fragments arranged by size:
3828 933 384 306 302 171 108 69
36 33 30 27
Bpu10I CC'TnA_GC
Cuts at: 1376 1679 1775 2218 2966 1376
Size: 303 96 443 748 4637
Fragments arranged by size:
4637 748 443 303 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2860 2989 3403 4282 4996 5294 2860
Size: 129 414 879 714 298 3793
Fragments arranged by size:
3793 879 714 414 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 308 499 1057 1618 2032 5931 308
Size: 191 558 561 414 3899 604
Fragments arranged by size:
3899 604 561 558 414 191
BsaAI yAC'GTr
Cuts at: 1551 2007 2262 2276 3580 1551
Size: 456 255 14 1304 4198
Fragments arranged by size:
4198 1304 456 255 14
BsaBI GATnn'nnATC
Cuts at: 6 3741 5778 6
Size: 3735 2037 455
Fragments arranged by size:
3735 2037 455
BsaHI Gr'CG_yC
Cuts at: 475 1181 1255 1907 2526 3658 3906 4608 6104
Size: 706 74 652 619 1132 248 702 1496
Cuts at: 6104 475
Size: 598
Fragments arranged by size:
1496 1132 706 702 652 619 598 248
74
BsaWI w'CCGG_w
Cuts at: 299 1958 3058 3416 3937 4615 5026 5173 5447
Size: 1659 1100 358 521 678 411 147 274
Cuts at: 5447 299
Size: 1079
Fragments arranged by size:
1659 1100 1079 678 521 411 358 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 1471 1501 2101 2131 2533 2563 115
Size: 30 1326 30 600 30 402 30 3779
Fragments arranged by size:
3779 1326 600 402 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 859 1160 1393 1704 1891 2410 2554 859
Size: 301 233 311 187 519 144 4532
Fragments arranged by size:
4532 519 311 301 233 187 144
BseYI C'CCAG_C
Cuts at: 781 979 985 1164 1662 3652 4410 5075 5483
Size: 198 6 179 498 1990 758 665 408
Cuts at: 5483 781
Size: 1525
Fragments arranged by size:
1990 1525 758 665 498 408 198 179
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 153 2575 153
Size: 2422 3805
BsiEI CG_ry'CG
Cuts at: 104 867 3769 3815 5045 104
Size: 763 2902 46 1230 1286
Fragments arranged by size:
2902 1286 1230 763 46
BsiHKAI G_wGCw'C
Cuts at: 299 344 418 1058 1556 1866 2681 2951 3241
Size: 45 74 640 498 310 815 270 290
Cuts at: 3241 4019 4209 5069 299
Size: 778 190 860 1457
Fragments arranged by size:
1457 860 815 778 640 498 310 290
270 190 74 45
BsmI GAATG_Cn'
Cuts at: 2613 2613
Size: 6227
Bsp1286I G_dGCh'C
Cuts at: 299 344 397 418 599 1058 1082 1247 1556
Size: 45 53 21 181 459 24 165 309
Cuts at: 1556 1820 1866 1966 2072 2681 2951 3241 3852
Size: 264 46 100 106 609 270 290 611
Cuts at: 3852 3945 4019 4209 4271 5069 299
Size: 93 74 190 62 798 1457
Fragments arranged by size:
1457 798 611 609 459 309 290 270
264 190 181 165 106 100 93 74
62 53 46 45 24 21
BspEI T'CCGG_A
Cuts at: 3058 3058
Size: 6227
BspHI T'CATG_A
Cuts at: 1541 4659 1541
Size: 3118 3109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1271 1636 2055 2978 3277 3491 3793 4174 1271
Size: 365 419 923 299 214 302 381 3324
Fragments arranged by size:
3324 923 419 381 365 302 299 214
BsrBI CCG'CTC
Cuts at: 50 902 969 1707 1724 1782 1854 2460 2640
Size: 852 67 738 17 58 72 606 180
Cuts at: 2640 4519 4573 6057 6222 50
Size: 1879 54 1484 165 55
Fragments arranged by size:
1879 1484 852 738 606 180 165 72
67 58 55 54 17
BsrDI GCAATG_nn'
Cuts at: 2303 4139 2303
Size: 1836 4391
BsrFI r'CCGG_y
Cuts at: 299 2111 2198 4225 4406 299
Size: 1812 87 2027 181 2120
Fragments arranged by size:
2120 2027 1812 181 87
BssSI C'ACGA_G
Cuts at: 1813 2125 4498 5206 1813
Size: 312 2373 708 2834
Fragments arranged by size:
2834 2373 708 312
BstAPI GCAn_nnn'nTGC
Cuts at: 2553 2649 3604 2553
Size: 96 955 5176
Fragments arranged by size:
5176 955 96
BstBI TT'CG_AA
Cuts at: 4588 4588
Size: 6227
BstEII G'GTnAC_C
Cuts at: 2107 2777 2107
Size: 670 5557
BstXI CCAn_nnnn'nTGG
Cuts at: 751 1074 1224 751
Size: 323 150 5754
Fragments arranged by size:
5754 323 150
BstYI r'GATC_y
Cuts at: 1 1529 2328 3112 3252 3719 3742 4077 4323
Size: 1528 799 784 140 467 23 335 246
Cuts at: 4323 4629 4641 4727 5779 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1528 1052 799 784 467 449 335 306
246 140 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 389 1829 389
Size: 1440 4787
BtgI C'CryG_G
Cuts at: 1238 3050 1238
Size: 1812 4415
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2058 2940 4177 4358 4521 2058
Size: 882 1237 181 163 3764
Fragments arranged by size:
3764 1237 882 181 163
BtsI GCAGTG_nn'
Cuts at: 1079 1562 2662 2896 3470 1079
Size: 483 1100 234 574 3836
Fragments arranged by size:
3836 1100 574 483 234
DraI TTT'AAA
Cuts at: 3226 3226
Size: 6227
DrdI GACnn_nn'nnGTC
Cuts at: 3933 5277 3933
Size: 1344 4883
EaeI y'GGCC_r
Cuts at: 879 1308 1499 2405 3812 3986 4377 4404 5749
Size: 429 191 906 1407 174 391 27 1345
Cuts at: 5749 879
Size: 1357
Fragments arranged by size:
1407 1357 1345 906 429 391 191 174
27
EagI C'GGCC_G
Cuts at: 3812 3812
Size: 6227
EarI CTCTTCn'nnn_
Cuts at: 836 1114 1206 1681 2051 2366 2467 4250 4460
Size: 278 92 475 370 315 101 1783 210
Cuts at: 4460 836
Size: 2603
Fragments arranged by size:
2603 1783 475 370 315 278 210 101
92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 517 1536 1849 4435 5177 5323 6037 517
Size: 1019 313 2586 742 146 714 707
Fragments arranged by size:
2586 1019 742 714 707 313 146
EcoICRI GAG'CTC
Cuts at: 2679 2949 3239 2679
Size: 270 290 5667
Fragments arranged by size:
5667 290 270
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 251 553 937 965 1870 2117 2176 2209 2317
Size: 302 384 28 905 247 59 33 108
Cuts at: 2317 2488 2557 2587 2623 2650 3178 4052 4484
Size: 171 69 30 36 27 528 874 432
Cuts at: 4484 4837 5499 251
Size: 353 662 979
Fragments arranged by size:
979 905 874 662 528 432 384 353
302 247 171 108 69 59 36 33
30 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 226 388 1632 1774 2392 2788 2857 226
Size: 162 1244 142 618 396 69 3596
Fragments arranged by size:
3596 1244 618 396 162 142 69
EcoO109I rG'GnC_Cy
Cuts at: 219 1007 1078 1079 1767 1798 2517 2571 2646
Size: 788 71 1 688 31 719 54 75
Cuts at: 2646 3366 219
Size: 720 3080
Fragments arranged by size:
3080 788 720 719 688 75 71 54
31 1
EcoRI G'AATT_C
Cuts at: 3231 3231
Size: 6227
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1161 4246 1161
Size: 3085 3142
FspI TGC'GCA
Cuts at: 1945 4008 1945
Size: 2063 4164
HaeII r_GCGC'y
Cuts at: 854 1884 1893 2249 2529 2629 3661 3909 5139
Size: 1030 9 356 280 100 1032 248 1230
Cuts at: 5139 5910 854
Size: 771 1171
Fragments arranged by size:
1230 1171 1032 1030 771 356 280 248
100 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 188 372 404 1219 1251 2031 2063 2101
Size: 32 184 32 815 32 780 32 38
Cuts at: 2101 2133 2313 2345 2533 2565 3104 3136 4069
Size: 32 180 32 188 32 539 32 933
Cuts at: 4069 4099 4101 4131 156
Size: 30 2 30 2252
Fragments arranged by size:
2252 933 815 780 539 188 184 180
38 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2502 3266 2502
Size: 764 5463
KasI G'GCGC_C
Cuts at: 2525 3657 3905 2525
Size: 1132 248 4847
Fragments arranged by size:
4847 1132 248
KpnI G_GTAC'C
Cuts at: 3247 3247
Size: 6227
MluI A'CGCG_T
Cuts at: 5660 5660
Size: 6227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4986 5170 4986
Size: 184 6043
MscI TGG'CCA
Cuts at: 3988 3988
Size: 6227
MslI CAynn'nnrTG
Cuts at: 403 421 1951 4216 4343 403
Size: 18 1530 2265 127 2287
Fragments arranged by size:
2287 2265 1530 127 18
MspA1I CmG'CkG
Cuts at: 957 1613 2022 2217 2349 2404 2481 2559 3338
Size: 656 409 195 132 55 77 78 779
Cuts at: 3338 3652 4012 4796 5041 957
Size: 314 360 784 245 2143
Fragments arranged by size:
2143 784 779 656 409 360 314 245
195 132 78 77 55
NaeI GCC'GGC
Cuts at: 4408 4408
Size: 6227
NarI GG'CG_CC
Cuts at: 2526 3658 3906 2526
Size: 1132 248 4847
Fragments arranged by size:
4847 1132 248
NgoMIV G'CCGG_C
Cuts at: 4406 4406
Size: 6227
NruI TCG'CGA
Cuts at: 2959 2959
Size: 6227
NspI r_CATG'y
Cuts at: 1421 3280 3780 5383 1421
Size: 1859 500 1603 2265
Fragments arranged by size:
2265 1859 1603 500
PasI CC'CwG_GG
Cuts at: 1966 2229 1966
Size: 263 5964
PciI A'CATG_T
Cuts at: 5379 5379
Size: 6227
PflMI CCAn_nnn'nTGG
Cuts at: 716 2535 2628 716
Size: 1819 93 4315
Fragments arranged by size:
4315 1819 93
PfoI T'CCnGG_A
Cuts at: 2051 2051
Size: 6227
PmeI GTTT'AAAC
Cuts at: 3226 3226
Size: 6227
PmlI CAC'GTG
Cuts at: 1551 2007 2276 1551
Size: 456 269 5502
Fragments arranged by size:
5502 456 269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 4673
Size: 32 6195
PpuMI rG'GwC_Cy
Cuts at: 219 1007 2571 219
Size: 788 1564 3875
Fragments arranged by size:
3875 1564 788
PspOMI G'GGCC_C
Cuts at: 1078 1078
Size: 6227
PstI C_TGCA'G
Cuts at: 2210 2270 2549 2721 2891 3274 3959 2210
Size: 60 279 172 170 383 685 4478
Fragments arranged by size:
4478 685 383 279 172 170 60
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6227
PvuII CAG'CTG
Cuts at: 957 1613 2217 3652 4012 957
Size: 656 604 1435 360 3172
Fragments arranged by size:
3172 1435 656 604 360
RsrII CG'GwC_CG
Cuts at: 4422 4422
Size: 6227
SacI G_AGCT'C
Cuts at: 2681 2951 3241 2681
Size: 270 290 5667
Fragments arranged by size:
5667 290 270
SalI G'TCGA_C
Cuts at: 3264 3264
Size: 6227
SapI GCTCTTCn'nnn_
Cuts at: 1206 2467 4250 4460 1206
Size: 1261 1783 210 2973
Fragments arranged by size:
2973 1783 1261 210
SbfI CC_TGCA'GG
Cuts at: 2210 3274 2210
Size: 1064 5163
ScaI AGT'ACT
Cuts at: 2587 5744 2587
Size: 3157 3070
SfcI C'TryA_G
Cuts at: 33 1359 2206 2266 2545 2717 2887 3270 3955
Size: 1326 847 60 279 172 170 383 685
Cuts at: 3955 4923 5114 33
Size: 968 191 1146
Fragments arranged by size:
1326 1146 968 847 685 383 279 191
172 170 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 446 446
Size: 6227
SfoI GGC'GCC
Cuts at: 2527 3659 3907 2527
Size: 1132 248 4847
Fragments arranged by size:
4847 1132 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6227
SmaI CCC'GGG
Cuts at: 3249 3249
Size: 6227
SmlI C'TyrA_G
Cuts at: 2143 2875 3004 3382 4297 5011 5273 2143
Size: 732 129 378 915 714 262 3097
Fragments arranged by size:
3097 915 732 714 378 262 129
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6227
SphI G_CATG'C
Cuts at: 3280 3780 3280
Size: 500 5727
StuI AGG'CCT
Cuts at: 1033 1638 2176 3167 1033
Size: 605 538 991 4093
Fragments arranged by size:
4093 991 605 538
StyI C'CwwG_G
Cuts at: 119 509 626 737 1034 1132 3361 3715 119
Size: 390 117 111 297 98 2229 354 2631
Fragments arranged by size:
2631 2229 390 354 297 117 111 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 515 1825 3810 3943 4402 4611 292
Size: 223 1310 1985 133 459 209 1908
Fragments arranged by size:
1985 1908 1310 459 223 209 133
TatI w'GTAC_w
Cuts at: 2585 2927 5742 2585
Size: 342 2815 3070
Fragments arranged by size:
3070 2815 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 115 147 369 401 991 1023 1297 1329 1422
Size: 32 222 32 590 32 274 32 93
Cuts at: 1422 1454 5744 5776 115
Size: 32 4290 32 566
Fragments arranged by size:
4290 590 566 274 222 93 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4024 4024
Size: 6227
XbaI T'CTAG_A
Cuts at: 3258 3258
Size: 6227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3722 3722
Size: 6227
XhoI C'TCGA_G
Cuts at: 2143 2143
Size: 6227
XmaI C'CCGG_G
Cuts at: 3247 3247
Size: 6227
XmnI GAAnn'nnTTC
Cuts at: 181 181
Size: 6227
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AscI BaeI BfrBI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NsiI PacI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SgrAI SnaBI SrfI SspI SwaI
ZraI