Restriction map for
pF1KA0866
(Circular) (Six-base) MAPSORT of: pF1KA0866.seq Check: 7296 from: 1 to: 9029
pF1KA0866 9029 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 506 2634 3876 5667 506
Size: 2128 1242 1791 3868
Fragments arranged by size:
3868 2128 1791 1242
AatII G_ACGT'C
Cuts at: 863 863
Size: 9029
AccI GT'mk_AC
Cuts at: 524 4111 6067 524
Size: 3587 1956 3486
Fragments arranged by size:
3587 3486 1956
Acc65I G'GTAC_C
Cuts at: 6045 6045
Size: 9029
AclI AA'CG_TT
Cuts at: 8869 8869
Size: 9029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3457 3664 4366 4984 5659 5680 6854 7286 8033
Size: 207 702 618 675 21 1174 432 747
Cuts at: 8033 8301 3457
Size: 268 4185
Fragments arranged by size:
4185 1174 747 702 675 618 432 268
207 21
AflII C'TTAA_G
Cuts at: 2731 2731
Size: 9029
AflIII A'CryG_T
Cuts at: 527 4106 6381 7485 8462 527
Size: 3579 2275 1104 977 1094
Fragments arranged by size:
3579 2275 1104 1094 977
AgeI A'CCGG_T
Cuts at: 7417 7417
Size: 9029
AhdI GACnn_n'nnGTC
Cuts at: 1215 4038 1215
Size: 2823 6206
AleI CACnn'nnGTG
Cuts at: 1423 2626 1423
Size: 1203 7826
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2233 4271 5830 2233
Size: 2038 1559 5432
Fragments arranged by size:
5432 2038 1559
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 805 837 1711 1743 3098 3130 805
Size: 32 874 32 1355 32 6704
Fragments arranged by size:
6704 1355 874 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 690 2544 2824 2865 2964 3012 3264 3792 3897
Size: 1854 280 41 99 48 252 528 105
Cuts at: 3897 4074 4191 4392 6334 7901 690
Size: 177 117 201 1942 1567 1818
Fragments arranged by size:
1942 1854 1818 1567 528 280 252 201
177 117 105 99 48 41
ApaI G_GGCC'C
Cuts at: 3930 4581 4716 3930
Size: 651 135 8243
Fragments arranged by size:
8243 651 135
ApaLI G'TGCA_C
Cuts at: 1767 7799 1767
Size: 6032 2997
ApoI r'AATT_y
Cuts at: 838 4462 6033 8382 8638 838
Size: 3624 1571 2349 256 1229
Fragments arranged by size:
3624 2349 1571 1229 256
AseI AT'TA_AT
Cuts at: 20 1268 20
Size: 1248 7781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9029
AvaI C'yCGr_G
Cuts at: 3929 4574 4612 4853 5230 5476 5987 6049 3929
Size: 645 38 241 377 246 511 62 6909
Fragments arranged by size:
6909 645 511 377 246 241 62 38
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 717 750 717
Size: 33 8996
BamHI G'GATC_C
Cuts at: 1475 6054 1475
Size: 4579 4450
BanI G'GyrC_C
Cuts at: 6045 6459 6707 6742 6045
Size: 414 248 35 8332
Fragments arranged by size:
8332 414 248 35
BanII G_rGCy'C
Cuts at: 1670 2020 3350 3611 3731 3930 3941 4581 4716
Size: 350 1330 261 120 199 11 640 135
Cuts at: 4716 5214 5498 6043 7073 1670
Size: 498 284 545 1030 3626
Fragments arranged by size:
3626 1330 1030 640 545 498 350 284
261 199 135 120 11
BbeI G_GCGC'C
Cuts at: 6463 6711 6463
Size: 248 8781
BbsI GAAGACnn'nnnn_
Cuts at: 1179 2259 2701 2918 3581 4655 4747 4931 1179
Size: 1080 442 217 663 1074 92 184 5277
Fragments arranged by size:
5277 1080 1074 663 442 217 184 92
BbvCI CC'TCA_GC
Cuts at: 2825 3035 4529 2825
Size: 210 1494 7325
Fragments arranged by size:
7325 1494 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 837 871 837
Size: 34 8995
BciVI GTATCCnnnnn_n'
Cuts at: 1406 6927 7688 9029 1406
Size: 5521 761 1341 1406
Fragments arranged by size:
5521 1406 1341 761
BclI T'GATC_A
Cuts at: 2357 3081 4467 6549 2357
Size: 724 1386 2082 4837
Fragments arranged by size:
4837 2082 1386 724
BglI GCCn_nnn'nGGC
Cuts at: 700 2246 2488 2518 700
Size: 1546 242 30 7211
Fragments arranged by size:
7211 1546 242 30
BglII A'GATC_T
Cuts at: 1499 5117 6544 8581 1499
Size: 3618 1427 2037 1947
Fragments arranged by size:
3618 2037 1947 1427
BlpI GC'TnA_GC
Cuts at: 5413 6141 5413
Size: 728 8301
Bme1580I G_kGCm'C
Cuts at: 1055 1771 3930 4581 4716 4947 5466 6654 6747
Size: 716 2159 651 135 231 519 1188 93
Cuts at: 6747 7803 1055
Size: 1056 2281
Fragments arranged by size:
2281 2159 1188 1056 716 651 519 231
135 93
BmgBI CAC'GTC
Cuts at: 389 1971 4035 4674 389
Size: 1582 2064 639 4744
Fragments arranged by size:
4744 2064 1582 639
BmrI ACTGGGnnnn_n'
Cuts at: 172 204 6241 6322 6657 8801 172
Size: 32 6037 81 335 2144 400
Fragments arranged by size:
6037 2144 400 335 81 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1530 7396 1530
Size: 5866 3163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 802 995 1609 1676 2215 2887 2920 3004
Size: 134 193 614 67 539 672 33 84
Cuts at: 3004 3076 3286 3307 3313 3460 3475 3571 3814
Size: 72 210 21 6 147 15 96 243
Cuts at: 3814 3898 4042 4150 4213 4717 4738 4780 5014
Size: 84 144 108 63 504 21 42 234
Cuts at: 5014 5091 5272 5329 5350 5362 5584 5785 5954
Size: 77 181 57 21 12 222 201 169
Cuts at: 5954 668
Size: 3743
Fragments arranged by size:
3743 672 614 539 504 243 234 222
210 201 193 181 169 147 144 134
108 96 84 84 77 72 67 63
57 42 33 21 21 21 15 12
6
Bpu10I CC'TnA_GC
Cuts at: 2825 3035 3610 3683 4529 5890 2825
Size: 210 575 73 846 1361 5964
Fragments arranged by size:
5964 1361 846 575 210 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1798 2572 3040 3139 3334 3763 4807 5737 5863
Size: 774 468 99 195 429 1044 930 126
Cuts at: 5863 6205 7084 7576 7874 1798
Size: 342 879 492 298 2953
Fragments arranged by size:
2953 1044 930 879 774 492 468 429
342 298 195 126 99
BsaI GGTCTCn'nnnn_
Cuts at: 886 3527 4714 5899 8733 886
Size: 2641 1187 1185 2834 1182
Fragments arranged by size:
2834 2641 1187 1185 1182
BsaAI yAC'GTr
Cuts at: 455 6382 455
Size: 5927 3102
BsaBI GATnn'nnATC
Cuts at: 6 6543 8580 6
Size: 6537 2037 455
Fragments arranged by size:
6537 2037 455
BsaHI Gr'CG_yC
Cuts at: 860 1216 2426 4039 4787 6460 6708 7410 8906
Size: 356 1210 1613 748 1673 248 702 1496
Cuts at: 8906 860
Size: 983
Fragments arranged by size:
1673 1613 1496 1210 983 748 702 356
248
BsaWI w'CCGG_w
Cuts at: 593 2258 5498 6218 6739 7417 7691 7838 8249
Size: 1665 3240 720 521 678 274 147 411
Cuts at: 8249 593
Size: 1373
Fragments arranged by size:
3240 1665 1373 720 678 521 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 373 388 403 418 607 637 805 835 1750
Size: 15 15 15 189 30 168 30 915
Cuts at: 1750 1780 3098 3128 3886 3916 373
Size: 30 1318 30 758 30 5486
Fragments arranged by size:
5486 1318 915 758 189 168 30 30
30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 277 696 813 1141 1144 1292 1612 1926 2458
Size: 419 117 328 3 148 320 314 532
Cuts at: 2458 2461 2674 2677 2782 2842 2884 2917 2920
Size: 3 213 3 105 60 42 33 3
Cuts at: 2920 3001 3004 3136 3388 3391 3559 3739 4147
Size: 81 3 132 252 3 168 180 408
Cuts at: 4147 4150 4183 4348 4498 4747 4885 4893 5266
Size: 3 33 165 150 249 138 8 373
Cuts at: 5266 5347 5350 5359 5362 5602 5782 5791 5794
Size: 81 3 9 3 240 180 9 3
Cuts at: 5794 5986 277
Size: 192 3320
Fragments arranged by size:
3320 532 419 408 373 328 320 314
252 249 240 213 192 180 180 168
165 150 148 138 132 117 105 81
81 60 42 33 33 9 9 8
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 1059 1849 2026 2088 2954 4052 4322 5324 5786
Size: 790 177 62 866 1098 270 1002 462
Cuts at: 5786 6454 7212 7789 8285 1059
Size: 668 758 577 496 1803
Fragments arranged by size:
1803 1098 1002 866 790 758 668 577
496 462 270 177 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3910 3930 3910
Size: 20 9009
BsiEI CG_ry'CG
Cuts at: 104 1681 4346 5379 6571 6617 7825 104
Size: 1577 2665 1033 1192 46 1208 1308
Fragments arranged by size:
2665 1577 1308 1208 1192 1033 46
BsiHKAI G_wGCw'C
Cuts at: 1670 1771 2020 3350 3611 3731 3941 5498 6043
Size: 101 249 1330 261 120 210 1557 545
Cuts at: 6043 6821 7011 7803 1670
Size: 778 190 792 2896
Fragments arranged by size:
2896 1557 1330 792 778 545 261 249
210 190 120 101
BsmI GAATG_Cn'
Cuts at: 1726 2228 2313 2348 1726
Size: 502 85 35 8407
Fragments arranged by size:
8407 502 85 35
BsmBI CGTCTCn'nnnn_
Cuts at: 929 3658 4906 5984 929
Size: 2729 1248 1078 3974
Fragments arranged by size:
3974 2729 1248 1078
Bsp1286I G_dGCh'C
Cuts at: 1055 1670 1771 2020 3350 3611 3731 3930 3941
Size: 615 101 249 1330 261 120 199 11
Cuts at: 3941 4581 4716 4947 5214 5466 5498 6043 6654
Size: 640 135 231 267 252 32 545 611
Cuts at: 6654 6747 6821 7011 7073 7803 1055
Size: 93 74 190 62 730 2281
Fragments arranged by size:
2281 1330 730 640 615 611 545 267
261 252 249 231 199 190 135 120
101 93 74 62 32 11
BspEI T'CCGG_A
Cuts at: 5498 8249 5498
Size: 2751 6278
BspHI T'CATG_A
Cuts at: 2354 8205 2354
Size: 5851 3178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 506 2634 2786 3876 5583 5667 6079 6293 6595
Size: 2128 152 1090 1707 84 412 214 302
Cuts at: 6595 6976 506
Size: 381 2559
Fragments arranged by size:
2559 2128 1707 1090 412 381 302 214
152 84
BsrBI CCG'CTC
Cuts at: 50 429 2706 3498 3924 4140 4617 5481 7321
Size: 379 2277 792 426 216 477 864 1840
Cuts at: 7321 7375 8859 9024 50
Size: 54 1484 165 55
Fragments arranged by size:
2277 1840 1484 864 792 477 426 379
216 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2217 5739 6941 2217
Size: 3522 1202 4305
Fragments arranged by size:
4305 3522 1202
BsrFI r'CCGG_y
Cuts at: 2240 7027 7208 7417 2240
Size: 4787 181 209 3852
Fragments arranged by size:
4787 3852 209 181
BsrGI T'GTAC_A
Cuts at: 530 2075 530
Size: 1545 7484
BssSI C'ACGA_G
Cuts at: 550 2155 3310 4432 4903 5512 7300 7658 550
Size: 1605 1155 1122 471 609 1788 358 1921
Fragments arranged by size:
1921 1788 1605 1155 1122 609 471 358
BstAPI GCAn_nnn'nTGC
Cuts at: 555 3012 3897 6406 555
Size: 2457 885 2509 3178
Fragments arranged by size:
3178 2509 2457 885
BstBI TT'CG_AA
Cuts at: 4835 7390 4835
Size: 2555 6474
BstXI CCAn_nnnn'nTGG
Cuts at: 1849 1893 4023 4035 4266 5160 1849
Size: 44 2130 12 231 894 5718
Fragments arranged by size:
5718 2130 894 231 44 12
BstYI r'GATC_y
Cuts at: 1 1288 1475 1499 2294 3065 3443 4842 5117
Size: 1287 187 24 795 771 378 1399 275
Cuts at: 5117 6054 6521 6544 6879 7125 8137 8223 8235
Size: 937 467 23 335 246 1012 86 12
Cuts at: 8235 8581 1
Size: 346 449
Fragments arranged by size:
1399 1287 1012 937 795 771 467 449
378 346 335 275 246 187 86 24
23 12
Bsu36I CC'TnA_GG
Cuts at: 421 421
Size: 9029
BtgI C'CryG_G
Cuts at: 107 695 1094 1103 1739 4265 4778 5837 5855
Size: 588 399 9 636 2526 513 1059 18
Cuts at: 5855 107
Size: 3281
Fragments arranged by size:
3281 2526 1059 636 588 513 399 18
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 561 2506 3929 5538 6979 7160 7323 561
Size: 1945 1423 1609 1441 181 163 2267
Fragments arranged by size:
2267 1945 1609 1441 1423 181 163
BtsI GCAGTG_nn'
Cuts at: 2611 2635 6272 2611
Size: 24 3637 5368
Fragments arranged by size:
5368 3637 24
DraI TTT'AAA
Cuts at: 6028 6028
Size: 9029
DrdI GACnn_nn'nnGTC
Cuts at: 6735 7593 6735
Size: 858 8171
EaeI y'GGCC_r
Cuts at: 692 1379 1952 2000 2510 2849 3362 3395 3815
Size: 687 573 48 510 339 513 33 420
Cuts at: 3815 4025 4343 5258 5426 5624 5699 6614 6788
Size: 210 318 915 168 198 75 915 174
Cuts at: 6788 7179 7206 8551 692
Size: 391 27 1345 1170
Fragments arranged by size:
1345 1170 915 915 687 573 513 510
420 391 339 318 210 198 174 168
75 48 33 27
EagI C'GGCC_G
Cuts at: 4343 6614 4343
Size: 2271 6758
EarI CTCTTCn'nnn_
Cuts at: 472 538 1570 2155 3152 3239 3244 3656 4274
Size: 66 1032 585 997 87 5 412 618
Cuts at: 4274 4285 4503 4580 4748 4870 7052 7262 472
Size: 11 218 77 168 122 2182 210 2239
Fragments arranged by size:
2239 2182 1032 997 618 585 412 218
210 168 122 87 77 66 11 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 394 2269 3949 7237 7547 7693 8839 394
Size: 1875 1680 3288 310 146 1146 584
Fragments arranged by size:
3288 1875 1680 1146 584 310 146
EcoICRI GAG'CTC
Cuts at: 1668 2018 3348 3609 3729 3939 5496 6041 1668
Size: 350 1330 261 120 210 1557 545 4656
Fragments arranged by size:
4656 1557 1330 545 350 261 210 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 802 995 1609 1676 2215 2887 2920 3004
Size: 134 193 614 67 539 672 33 84
Cuts at: 3004 3076 3286 3307 3313 3457 3460 3475 3571
Size: 72 210 21 6 144 3 15 96
Cuts at: 3571 3664 3814 3898 4042 4150 4213 4366 4717
Size: 93 150 84 144 108 63 153 351
Cuts at: 4717 4738 4780 4984 5014 5091 5272 5329 5350
Size: 21 42 204 30 77 181 57 21
Cuts at: 5350 5362 5584 5659 5680 5785 5954 6854 7286
Size: 12 222 75 21 105 169 900 432
Cuts at: 7286 8033 8301 668
Size: 747 268 1396
Fragments arranged by size:
1396 900 747 672 614 539 432 351
268 222 210 204 193 181 169 153
150 144 144 134 108 105 96 93
84 84 77 75 72 67 63 57
42 33 30 21 21 21 21 15
12 6 3
EcoNI CCTnn'n_nnAGG
Cuts at: 1591 1963 2443 2782 2902 3478 3541 3838 4585
Size: 372 480 339 120 576 63 297 747
Cuts at: 4585 5233 1591
Size: 648 5387
Fragments arranged by size:
5387 747 648 576 480 372 339 297
120 63
EcoO109I rG'GnC_Cy
Cuts at: 219 3483 3555 3651 3831 4560 4578 4713 4980
Size: 3264 72 96 180 729 18 135 267
Cuts at: 4980 5190 6168 219
Size: 210 978 3080
Fragments arranged by size:
3264 3080 978 729 267 210 180 135
96 72 18
EcoRI G'AATT_C
Cuts at: 6033 6033
Size: 9029
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 165 255 5685 7048 165
Size: 90 5430 1363 2146
Fragments arranged by size:
5430 2146 1363 90
FspI TGC'GCA
Cuts at: 6810 6810
Size: 9029
HaeII r_GCGC'y
Cuts at: 1401 2033 4200 4822 5304 5563 6463 6711 7733
Size: 632 2167 622 482 259 900 248 1022
Cuts at: 7733 8712 1401
Size: 979 1718
Fragments arranged by size:
2167 1718 1022 979 900 632 622 482
259 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 546 578 607 639 808 840 1123 1155 1201
Size: 32 29 32 169 32 283 32 46
Cuts at: 1201 1233 1280 1312 1484 1516 2149 2181 3475
Size: 32 47 32 172 32 633 32 1294
Cuts at: 3475 3507 3655 3687 3886 3918 4024 4056 5102
Size: 32 148 32 199 32 106 32 1046
Cuts at: 5102 5134 5767 5799 6871 6901 6903 6933 546
Size: 32 633 32 1072 30 2 30 2642
Fragments arranged by size:
2642 1294 1072 1046 633 633 283 199
172 169 148 106 47 46 32 32
32 32 32 32 32 32 32 32
32 32 32 32 30 30 29 2
HincII GTy'rAC
Cuts at: 483 525 4809 6068 483
Size: 42 4284 1259 3444
Fragments arranged by size:
4284 3444 1259 42
KasI G'GCGC_C
Cuts at: 6459 6707 6459
Size: 248 8781
KpnI G_GTAC'C
Cuts at: 6049 6049
Size: 9029
MluI A'CGCG_T
Cuts at: 8462 8462
Size: 9029
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1964 4253 4463 7700 7884 1964
Size: 2289 210 3237 184 3109
Fragments arranged by size:
3237 3109 2289 210 184
MscI TGG'CCA
Cuts at: 1381 2002 2512 3364 3397 4027 5260 5428 6790
Size: 621 510 852 33 630 1233 168 1362
Cuts at: 6790 1381
Size: 3620
Fragments arranged by size:
3620 1362 1233 852 630 621 510 168
33
MslI CAynn'nnrTG
Cuts at: 1423 2353 2557 2626 3988 4907 7018 7145 1423
Size: 930 204 69 1362 919 2111 127 3307
Fragments arranged by size:
3307 2111 1362 930 919 204 127 69
MspA1I CmG'CkG
Cuts at: 1237 1409 1527 1902 2073 2088 2202 2544 2760
Size: 172 118 375 171 15 114 342 216
Cuts at: 2760 2793 2982 3360 4128 4161 4425 4749 4954
Size: 33 189 378 768 33 264 324 205
Cuts at: 4954 5203 5328 5763 5820 6140 6454 6814 7827
Size: 249 125 435 57 320 314 360 1013
Cuts at: 7827 8072 1237
Size: 245 2194
Fragments arranged by size:
2194 1013 768 435 378 375 360 342
324 320 314 264 249 245 216 205
189 172 171 125 118 114 57 33
33 15
NaeI GCC'GGC
Cuts at: 2242 7210 2242
Size: 4968 4061
NarI GG'CG_CC
Cuts at: 6460 6708 6460
Size: 248 8781
NcoI C'CATG_G
Cuts at: 107 1103 5855 107
Size: 996 4752 3281
Fragments arranged by size:
4752 3281 996
NdeI CA'TA_TG
Cuts at: 1392 1392
Size: 9029
NgoMIV G'CCGG_C
Cuts at: 2240 7208 2240
Size: 4968 4061
NotI GC'GGCC_GC
Cuts at: 4343 4343
Size: 9029
NspI r_CATG'y
Cuts at: 531 603 2055 4815 6082 6582 7489 531
Size: 72 1452 2760 1267 500 907 2071
Fragments arranged by size:
2760 2071 1452 1267 907 500 72
PciI A'CATG_T
Cuts at: 527 7485 527
Size: 6958 2071
PflMI CCAn_nnn'nTGG
Cuts at: 1260 4695 5517 1260
Size: 3435 822 4772
Fragments arranged by size:
4772 3435 822
PfoI T'CCnGG_A
Cuts at: 1085 1478 1586 2270 3446 3476 3689 4058 5288
Size: 393 108 684 1176 30 213 369 1230
Cuts at: 5288 1085
Size: 4826
Fragments arranged by size:
4826 1230 1176 684 393 369 213 108
30
PmeI GTTT'AAAC
Cuts at: 6028 6028
Size: 9029
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 805 837 3098 3130 8168 8200 805
Size: 32 2261 32 5038 32 1634
Fragments arranged by size:
5038 2261 1634 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 219 3483 3831 4980 5190 219
Size: 3264 348 1149 210 4058
Fragments arranged by size:
4058 3264 1149 348 210
PspOMI G'GGCC_C
Cuts at: 3926 4577 4712 3926
Size: 651 135 8243
Fragments arranged by size:
8243 651 135
PspXI vC'TCGA_Gb
Cuts at: 4612 5476 4612
Size: 864 8165
PstI C_TGCA'G
Cuts at: 1172 1559 2645 2693 2798 3386 3902 3965 4250
Size: 387 1086 48 105 588 516 63 285
Cuts at: 4250 4892 4959 5060 5678 5819 6076 6761 1172
Size: 642 67 101 618 141 257 685 3440
Fragments arranged by size:
3440 1086 685 642 618 588 516 387
285 257 141 105 101 67 63 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9029
PvuII CAG'CTG
Cuts at: 1237 1527 2073 2088 2202 2544 2760 2793 2982
Size: 290 546 15 114 342 216 33 189
Cuts at: 2982 3360 4128 4161 4749 4954 5328 5763 6454
Size: 378 768 33 588 205 374 435 691
Cuts at: 6454 6814 1237
Size: 360 3452
Fragments arranged by size:
3452 768 691 588 546 435 378 374
360 342 290 216 205 189 114 33
33 15
RsrII CG'GwC_CG
Cuts at: 7224 7224
Size: 9029
SacI G_AGCT'C
Cuts at: 1670 2020 3350 3611 3731 3941 5498 6043 1670
Size: 350 1330 261 120 210 1557 545 4656
Fragments arranged by size:
4656 1557 1330 545 350 261 210 120
SalI G'TCGA_C
Cuts at: 523 6066 523
Size: 5543 3486
SanDI GG'GwC_CC
Cuts at: 219 219
Size: 9029
SapI GCTCTTCn'nnn_
Cuts at: 1570 3244 4748 7052 7262 1570
Size: 1674 1504 2304 210 3337
Fragments arranged by size:
3337 2304 1674 1504 210
SbfI CC_TGCA'GG
Cuts at: 6076 6076
Size: 9029
ScaI AGT'ACT
Cuts at: 8546 8546
Size: 9029
SexAI A'CCwGG_T
Cuts at: 4400 4652 4400
Size: 252 8777
SfcI C'TryA_G
Cuts at: 33 1168 1367 1555 1651 2641 2689 2782 2794
Size: 1135 199 188 96 990 48 93 12
Cuts at: 2794 2929 3382 3898 3961 4246 4888 4955 5056
Size: 135 453 516 63 285 642 67 101
Cuts at: 5056 5674 5815 6072 6757 7750 7941 33
Size: 618 141 257 685 993 191 1121
Fragments arranged by size:
1135 1121 993 990 685 642 618 516
453 285 257 199 191 188 141 135
101 96 93 67 63 48 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 700 700
Size: 9029
SfoI GGC'GCC
Cuts at: 6461 6709 6461
Size: 248 8781
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9029
SmaI CCC'GGG
Cuts at: 3931 4855 6051 3931
Size: 924 1196 6909
Fragments arranged by size:
6909 1196 924
SmlI C'TyrA_G
Cuts at: 1813 2587 2731 3019 3118 3349 3742 4612 4822
Size: 774 144 288 99 231 393 870 210
Cuts at: 4822 5230 5476 5752 5878 5987 6184 7099 7591
Size: 408 246 276 126 109 197 915 492
Cuts at: 7591 7853 1813
Size: 262 2989
Fragments arranged by size:
2989 915 870 774 492 408 393 288
276 262 246 231 210 197 144 126
109 99
SnaBI TAC'GTA
Cuts at: 455 455
Size: 9029
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9029
SphI G_CATG'C
Cuts at: 603 6082 6582 603
Size: 5479 500 3050
Fragments arranged by size:
5479 3050 500
SrfI GCCC'GGGC
Cuts at: 3931 3931
Size: 9029
StuI AGG'CCT
Cuts at: 2344 4591 2344
Size: 2247 6782
StyI C'CwwG_G
Cuts at: 107 362 980 1103 1382 2675 2699 3761 4028
Size: 255 618 123 279 1293 24 1062 267
Cuts at: 4028 4556 4592 4664 5855 6011 6163 6517 107
Size: 528 36 72 1191 156 152 354 2619
Fragments arranged by size:
2619 1293 1191 1062 618 528 354 279
267 255 156 152 123 72 36 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 366 1152 1695 2779 4081 4336 6612 6745 7204
Size: 786 543 1084 1302 255 2276 133 459
Cuts at: 7204 7413 366
Size: 209 1982
Fragments arranged by size:
2276 1982 1302 1084 786 543 459 255
209 133
TatI w'GTAC_w
Cuts at: 530 2075 5708 8544 530
Size: 1545 3633 2836 1015
Fragments arranged by size:
3633 2836 1545 1015
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 1256 1288 1750 1782 8546 8578 373
Size: 32 851 32 462 32 6764 32 824
Fragments arranged by size:
6764 851 824 462 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3669 3949 4654 5380 6826 3669
Size: 280 705 726 1446 5872
Fragments arranged by size:
5872 1446 726 705 280
XbaI T'CTAG_A
Cuts at: 5923 6060 5923
Size: 137 8892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1110 1149 1584 2325 3852 4188 4671 4713 4797
Size: 39 435 741 1527 336 483 42 84
Cuts at: 4797 5559 6524 1110
Size: 762 965 3615
Fragments arranged by size:
3615 1527 965 762 741 483 435 336
84 42 39
XhoI C'TCGA_G
Cuts at: 4612 5230 5476 5987 4612
Size: 618 246 511 7654
Fragments arranged by size:
7654 618 511 246
XmaI C'CCGG_G
Cuts at: 3929 4853 6049 3929
Size: 924 1196 6909
Fragments arranged by size:
6909 1196 924
XmnI GAAnn'nnTTC
Cuts at: 1516 2228 4294 1516
Size: 712 2066 6251
Fragments arranged by size:
6251 2066 712
ZraI GAC'GTC
Cuts at: 861 861
Size: 9029
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NotI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BmtI BsiWI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NruI NsiI PacI PasI PmlI
PshAI PsiI PsrI SacII SgrAI SspI SwaI