Restriction map for
pF1KA0869
(Circular) (Six-base) MAPSORT of: pF1KA0869.seq Check: 6628 from: 1 to: 6452
pF1KA0869 6452 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1341 1341
Size: 6452
AatII G_ACGT'C
Cuts at: 3446 3446
Size: 6452
AccI GT'mk_AC
Cuts at: 173 2437 3490 173
Size: 2264 1053 3135
Fragments arranged by size:
3135 2264 1053
Acc65I G'GTAC_C
Cuts at: 2750 3468 2750
Size: 718 5734
AclI AA'CG_TT
Cuts at: 6292 6292
Size: 6452
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 649 712 1081 1180 1466 2021 2125 3163
Size: 234 63 369 99 286 555 104 1038
Cuts at: 3163 3274 4277 4709 5062 5724 415
Size: 111 1003 432 353 662 1143
Fragments arranged by size:
1143 1038 1003 662 555 432 369 353
286 234 111 104 99 63
AflII C'TTAA_G
Cuts at: 2337 2337
Size: 6452
AflIII A'CryG_T
Cuts at: 2921 3804 5604 5885 2921
Size: 883 1800 281 3488
Fragments arranged by size:
3488 1800 883 281
AhdI GACnn_n'nnGTC
Cuts at: 867 885 2547 3007 867
Size: 18 1662 460 4312
Fragments arranged by size:
4312 1662 460 18
AleI CACnn'nnGTG
Cuts at: 2278 2812 3281 2278
Size: 534 469 5449
Fragments arranged by size:
5449 534 469
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 518 518
Size: 6452
AlwNI CAG_nnn'CTG
Cuts at: 468 498 690 939 1404 1668 1990 2028 2044
Size: 30 192 249 465 264 322 38 16
Cuts at: 2044 2530 2619 3757 5195 468
Size: 486 89 1138 1438 1725
Fragments arranged by size:
1725 1438 1138 486 465 322 264 249
192 89 38 30 16
ApaLI G'TGCA_C
Cuts at: 431 3328 5290 431
Size: 2897 1962 1593
Fragments arranged by size:
2897 1962 1593
ApoI r'AATT_y
Cuts at: 2090 3456 5805 6061 2090
Size: 1366 2349 256 2481
Fragments arranged by size:
2481 2349 1366 256
AseI AT'TA_AT
Cuts at: 20 2140 20
Size: 2120 4332
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6452
AvaI C'yCGr_G
Cuts at: 359 614 966 1259 1590 3472 359
Size: 255 352 293 331 1882 3339
Fragments arranged by size:
3339 1882 352 331 293 255
BamHI G'GATC_C
Cuts at: 354 1426 3477 354
Size: 1072 2051 3329
Fragments arranged by size:
3329 2051 1072
BanI G'GyrC_C
Cuts at: 1684 2392 2750 2839 3468 3882 4130 4165 1684
Size: 708 358 89 629 414 248 35 3971
Fragments arranged by size:
3971 708 629 414 358 248 89 35
BanII G_rGCy'C
Cuts at: 529 952 1450 2495 3152 3466 4496 529
Size: 423 498 1045 657 314 1030 2485
Fragments arranged by size:
2485 1045 1030 657 498 423 314
BbeI G_GCGC'C
Cuts at: 2396 3886 4134 2396
Size: 1490 248 4714
Fragments arranged by size:
4714 1490 248
BbsI GAAGACnn'nnnn_
Cuts at: 1817 2338 2630 1817
Size: 521 292 5639
Fragments arranged by size:
5639 521 292
BbvCI CC'TCA_GC
Cuts at: 3238 3238
Size: 6452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6418
BciVI GTATCCnnnnn_n'
Cuts at: 2313 4350 5406 6452 2313
Size: 2037 1056 1046 2313
Fragments arranged by size:
2313 2037 1056 1046
BclI T'GATC_A
Cuts at: 3972 3972
Size: 6452
BglI GCCn_nnn'nGGC
Cuts at: 421 2390 421
Size: 1969 4483
BglII A'GATC_T
Cuts at: 443 816 3131 3967 6004 443
Size: 373 2315 836 2037 891
Fragments arranged by size:
2315 2037 891 836 373
BlpI GC'TnA_GC
Cuts at: 3564 3564
Size: 6452
Bme1580I G_kGCm'C
Cuts at: 435 2842 3332 4077 4170 5294 435
Size: 2407 490 745 93 1124 1593
Fragments arranged by size:
2407 1593 1124 745 490 93
BmgBI CAC'GTC
Cuts at: 1436 1436
Size: 6452
BmrI ACTGGGnnnn_n'
Cuts at: 2038 3664 3745 4080 6224 2038
Size: 1626 81 335 2144 2266
Fragments arranged by size:
2266 2144 1626 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1847 2456 2748 4819 1847
Size: 609 292 2071 3480
Fragments arranged by size:
3480 2071 609 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 634 925 1237 1354 1585 1900 2012 2602
Size: 144 291 312 117 231 315 112 590
Cuts at: 2602 2652 3016 3100 3268 490
Size: 50 364 84 168 3674
Fragments arranged by size:
3674 590 364 315 312 291 231 168
144 117 112 84 50
Bpu10I CC'TnA_GC
Cuts at: 722 749 3238 722
Size: 27 2489 3936
Fragments arranged by size:
3936 2489 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 1371 2479 3136 3628 4507 5221 5519 780
Size: 591 1108 657 492 879 714 298 1713
Fragments arranged by size:
1713 1108 879 714 657 591 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 549 875 2638 2770 2782 6156 549
Size: 326 1763 132 12 3374 845
Fragments arranged by size:
3374 1763 845 326 132 12
BsaAI yAC'GTr
Cuts at: 2922 3805 2922
Size: 883 5569
BsaBI GATnn'nnATC
Cuts at: 6 1135 3418 3966 6003 6
Size: 1129 2283 548 2037 455
Fragments arranged by size:
2283 2037 1129 548 455
BsaHI Gr'CG_yC
Cuts at: 2264 2393 3443 3883 4131 4833 6329 2264
Size: 129 1050 440 248 702 1496 2387
Fragments arranged by size:
2387 1496 1050 702 440 248 129
BsaWI w'CCGG_w
Cuts at: 2246 2828 3011 3641 4162 4840 5251 5398 5672
Size: 582 183 630 521 678 411 147 274
Cuts at: 5672 2246
Size: 3026
Fragments arranged by size:
3026 678 630 582 521 411 274 183
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2931 2961 2931
Size: 30 6422
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 1165 1464 1719 2656 2692 2695 2698 2707
Size: 1005 299 255 937 36 3 3 9
Cuts at: 2707 2710 2713 160
Size: 3 3 3899
Fragments arranged by size:
3899 1005 937 299 255 36 9 3
3 3 3
BseYI C'CCAG_C
Cuts at: 140 522 1370 1908 2754 3423 3877 4635 5300
Size: 382 848 538 846 669 454 758 665
Cuts at: 5300 5708 140
Size: 408 884
Fragments arranged by size:
884 848 846 758 669 665 538 454
408 382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 2041 1348
Size: 693 5759
BsiEI CG_ry'CG
Cuts at: 104 118 3994 4040 5270 104
Size: 14 3876 46 1230 1286
Fragments arranged by size:
3876 1286 1230 46 14
BsiHKAI G_wGCw'C
Cuts at: 435 952 2495 2875 3152 3278 3332 3466 4244
Size: 517 1543 380 277 126 54 134 778
Cuts at: 4244 4434 5294 435
Size: 190 860 1593
Fragments arranged by size:
1593 1543 860 778 517 380 277 190
134 126 54
Bsp1286I G_dGCh'C
Cuts at: 435 529 952 1450 2495 2842 2875 3152 3278
Size: 94 423 498 1045 347 33 277 126
Cuts at: 3278 3332 3466 4077 4170 4244 4434 4496 5294
Size: 54 134 611 93 74 190 62 798
Cuts at: 5294 435
Size: 1593
Fragments arranged by size:
1593 1045 798 611 498 423 347 277
190 134 126 94 93 74 62 54
33
BspEI T'CCGG_A
Cuts at: 2246 3011 2246
Size: 765 5687
BspHI T'CATG_A
Cuts at: 4884 4884
Size: 6452
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1256 1341 2245 3098 3502 3716 4018 4399 1256
Size: 85 904 853 404 214 302 381 3309
Fragments arranged by size:
3309 904 853 404 381 302 214 85
BsrBI CCG'CTC
Cuts at: 50 240 2885 3271 4744 4798 6282 6447 50
Size: 190 2645 386 1473 54 1484 165 55
Fragments arranged by size:
2645 1484 1473 386 190 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4364 4364
Size: 6452
BsrFI r'CCGG_y
Cuts at: 1124 1576 1874 2420 2927 4450 4631 1124
Size: 452 298 546 507 1523 181 2945
Fragments arranged by size:
2945 1523 546 507 452 298 181
BssHII G'CGCG_C
Cuts at: 151 151
Size: 6452
BssSI C'ACGA_G
Cuts at: 655 1612 4723 5431 655
Size: 957 3111 708 1676
Fragments arranged by size:
3111 1676 957 708
BstAPI GCAn_nnn'nTGC
Cuts at: 660 1668 2080 2237 3829 660
Size: 1008 412 157 1592 3283
Fragments arranged by size:
3283 1592 1008 412 157
BstBI TT'CG_AA
Cuts at: 2985 4813 2985
Size: 1828 4624
BstXI CCAn_nnnn'nTGG
Cuts at: 1939 1939
Size: 6452
BstYI r'GATC_y
Cuts at: 1 354 443 581 816 1064 1426 3131 3477
Size: 353 89 138 235 248 362 1705 346
Cuts at: 3477 3944 3967 4302 4548 4854 4866 4952 6004
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6004 1
Size: 449
Fragments arranged by size:
1705 1052 467 449 362 353 346 335
306 248 246 235 138 89 86 23
12
Bsu36I CC'TnA_GG
Cuts at: 1410 2523 2822 1410
Size: 1113 299 5040
Fragments arranged by size:
5040 1113 299
BtgI C'CryG_G
Cuts at: 217 3180 217
Size: 2963 3489
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4402 4583 4746 4402
Size: 181 163 6108
Fragments arranged by size:
6108 181 163
BtsI GCAGTG_nn'
Cuts at: 602 2081 2579 3695 602
Size: 1479 498 1116 3359
Fragments arranged by size:
3359 1479 1116 498
ClaI AT'CG_AT
Cuts at: 701 701
Size: 6452
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 330 365 657 692 3188 3223 330
Size: 35 292 35 2496 35 3559
Fragments arranged by size:
3559 2496 292 35 35 35
DraI TTT'AAA
Cuts at: 3451 3451
Size: 6452
DrdI GACnn_nn'nnGTC
Cuts at: 169 4158 5502 169
Size: 3989 1344 1119
Fragments arranged by size:
3989 1344 1119
EaeI y'GGCC_r
Cuts at: 115 1073 1625 1653 4037 4211 4602 4629 5974
Size: 958 552 28 2384 174 391 27 1345
Cuts at: 5974 115
Size: 593
Fragments arranged by size:
2384 1345 958 593 552 391 174 28
27
EagI C'GGCC_G
Cuts at: 115 4037 115
Size: 3922 2530
EarI CTCTTCn'nnn_
Cuts at: 281 625 1028 2372 2581 2669 2702 4475 4685
Size: 344 403 1344 209 88 33 1773 210
Cuts at: 4685 281
Size: 2048
Fragments arranged by size:
2048 1773 1344 403 344 210 209 88
33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2425 3217 4660 5402 5548 6262 2425
Size: 792 1443 742 146 714 2615
Fragments arranged by size:
2615 1443 792 742 714 146
EcoICRI GAG'CTC
Cuts at: 950 2493 3150 3464 950
Size: 1543 657 314 3938
Fragments arranged by size:
3938 1543 657 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 415 490 634 649 712 925 1081 1180 1237
Size: 75 144 15 63 213 156 99 57
Cuts at: 1237 1354 1466 1585 1900 2012 2021 2125 2602
Size: 117 112 119 315 112 9 104 477
Cuts at: 2602 2652 3016 3100 3163 3268 3274 4277 4709
Size: 50 364 84 63 105 6 1003 432
Cuts at: 4709 5062 5724 415
Size: 353 662 1143
Fragments arranged by size:
1143 1003 662 477 432 364 353 315
213 156 144 119 117 112 112 105
104 99 84 75 63 63 57 50
15 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 133 2410 2522 133
Size: 2277 112 4063
Fragments arranged by size:
4063 2277 112
EcoO109I rG'GnC_Cy
Cuts at: 1245 2527 2905 3591 1245
Size: 1282 378 686 4106
Fragments arranged by size:
4106 1282 686 378
EcoRI G'AATT_C
Cuts at: 2090 3456 2090
Size: 1366 5086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 867 4471 867
Size: 3604 2848
FspI TGC'GCA
Cuts at: 4233 4233
Size: 6452
HaeII r_GCGC'y
Cuts at: 468 1282 1862 2396 3886 4134 5364 6135 468
Size: 814 580 534 1490 248 1230 771 785
Fragments arranged by size:
1490 1230 814 785 771 580 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 339 371 853 871 885 903 934 966 2241
Size: 32 482 18 14 18 31 32 1275
Cuts at: 2241 2273 2333 2365 2533 2565 2993 3025 4294
Size: 32 60 32 168 32 428 32 1269
Cuts at: 4294 4324 4326 4356 339
Size: 30 2 30 2435
Fragments arranged by size:
2435 1275 1269 482 428 168 60 32
32 32 32 32 32 31 30 30
18 18 14 2
HincII GTy'rAC
Cuts at: 621 2438 3491 621
Size: 1817 1053 3582
Fragments arranged by size:
3582 1817 1053
HindIII A'AGCT_T
Cuts at: 972 1078 972
Size: 106 6346
KasI G'GCGC_C
Cuts at: 2392 3882 4130 2392
Size: 1490 248 4714
Fragments arranged by size:
4714 1490 248
KpnI G_GTAC'C
Cuts at: 2754 3472 2754
Size: 718 5734
MfeI C'AATT_G
Cuts at: 3118 3118
Size: 6452
MluI A'CGCG_T
Cuts at: 5885 5885
Size: 6452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 2540 5211 5395 288
Size: 2252 2671 184 1345
Fragments arranged by size:
2671 2252 1345 184
MscI TGG'CCA
Cuts at: 1075 1627 4213 1075
Size: 552 2586 3314
Fragments arranged by size:
3314 2586 552
MslI CAynn'nnrTG
Cuts at: 1616 2278 2812 3281 4441 4568 1616
Size: 662 534 469 1160 127 3500
Fragments arranged by size:
3500 1160 662 534 469 127
MspA1I CmG'CkG
Cuts at: 206 219 1071 1215 1374 1524 1908 2151 2630
Size: 13 852 144 159 150 384 243 479
Cuts at: 2630 3162 3316 3563 3877 4237 5021 5266 206
Size: 532 154 247 314 360 784 245 1392
Fragments arranged by size:
1392 852 784 532 479 384 360 314
247 245 243 159 154 150 144 13
NaeI GCC'GGC
Cuts at: 2929 4633 2929
Size: 1704 4748
NarI GG'CG_CC
Cuts at: 2393 3883 4131 2393
Size: 1490 248 4714
Fragments arranged by size:
4714 1490 248
NdeI CA'TA_TG
Cuts at: 294 389 294
Size: 95 6357
NgoMIV G'CCGG_C
Cuts at: 2927 4631 2927
Size: 1704 4748
NspI r_CATG'y
Cuts at: 505 1398 3505 4005 5608 505
Size: 893 2107 500 1603 1349
Fragments arranged by size:
2107 1603 1349 893 500
PasI CC'CwG_GG
Cuts at: 1197 1932 2850 1197
Size: 735 918 4799
Fragments arranged by size:
4799 918 735
PciI A'CATG_T
Cuts at: 5604 5604
Size: 6452
PflMI CCAn_nnn'nTGG
Cuts at: 206 307 206
Size: 101 6351
PfoI T'CCnGG_A
Cuts at: 1562 1562
Size: 6452
PmeI GTTT'AAAC
Cuts at: 3451 3451
Size: 6452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 626 649 658 681 4898 4930 626
Size: 23 9 23 4217 32 2148
Fragments arranged by size:
4217 2148 32 23 23 9
PpuMI rG'GwC_Cy
Cuts at: 1245 2905 1245
Size: 1660 4792
PspXI vC'TCGA_Gb
Cuts at: 359 359
Size: 6452
PstI C_TGCA'G
Cuts at: 665 1253 1541 1664 3095 3438 3499 4184 665
Size: 588 288 123 1431 343 61 685 2933
Fragments arranged by size:
2933 1431 685 588 343 288 123 61
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6452
PvuII CAG'CTG
Cuts at: 206 1071 1215 1374 1524 1908 2151 2630 3162
Size: 865 144 159 150 384 243 479 532
Cuts at: 3162 3316 3877 4237 206
Size: 154 561 360 2421
Fragments arranged by size:
2421 865 561 532 479 384 360 243
159 154 150 144
RsrII CG'GwC_CG
Cuts at: 4647 4647
Size: 6452
SacI G_AGCT'C
Cuts at: 952 2495 3152 3466 952
Size: 1543 657 314 3938
Fragments arranged by size:
3938 1543 657 314
SacII CC_GC'GG
Cuts at: 220 220
Size: 6452
SalI G'TCGA_C
Cuts at: 2436 3489 2436
Size: 1053 5399
SapI GCTCTTCn'nnn_
Cuts at: 4475 4685 4475
Size: 210 6242
SbfI CC_TGCA'GG
Cuts at: 1253 1664 3095 3499 1253
Size: 411 1431 404 4206
Fragments arranged by size:
4206 1431 411 404
ScaI AGT'ACT
Cuts at: 5969 5969
Size: 6452
SexAI A'CCwGG_T
Cuts at: 404 449 404
Size: 45 6407
SfcI C'TryA_G
Cuts at: 33 661 1249 1537 1660 3091 3434 3495 4180
Size: 628 588 288 123 1431 343 61 685
Cuts at: 4180 5148 5339 33
Size: 968 191 1146
Fragments arranged by size:
1431 1146 968 685 628 588 343 288
191 123 61
SfoI GGC'GCC
Cuts at: 2394 3884 4132 2394
Size: 1490 248 4714
Fragments arranged by size:
4714 1490 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6452
SmaI CCC'GGG
Cuts at: 616 1261 3474 616
Size: 645 2213 3594
Fragments arranged by size:
3594 2213 645
SmlI C'TyrA_G
Cuts at: 359 759 1386 2337 2494 3151 3607 4522 5236
Size: 400 627 951 157 657 456 915 714
Cuts at: 5236 5498 359
Size: 262 1313
Fragments arranged by size:
1313 951 915 714 657 627 456 400
262 157
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6452
SphI G_CATG'C
Cuts at: 505 1398 3505 4005 505
Size: 893 2107 500 2952
Fragments arranged by size:
2952 2107 893 500
SspI AAT'ATT
Cuts at: 2138 2138
Size: 6452
StuI AGG'CCT
Cuts at: 1093 1093
Size: 6452
StyI C'CwwG_G
Cuts at: 1799 1848 3320 3586 3940 1799
Size: 49 1472 266 354 4311
Fragments arranged by size:
4311 1472 354 266 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1282 2020 2068 3021 3281 4035 4168 4627 4836
Size: 738 48 953 260 754 133 459 209
Cuts at: 4836 1282
Size: 2898
Fragments arranged by size:
2898 953 754 738 459 260 209 133
48
TatI w'GTAC_w
Cuts at: 363 5967 363
Size: 5604 848
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 5969 6001 2931
Size: 32 3006 32 3382
Fragments arranged by size:
3382 3006 32 32
Tth111I GACn'n_nGTC
Cuts at: 4249 4249
Size: 6452
XbaI T'CTAG_A
Cuts at: 3483 3483
Size: 6452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1350 3947 1350
Size: 2597 3855
XhoI C'TCGA_G
Cuts at: 359 359
Size: 6452
XmaI C'CCGG_G
Cuts at: 614 1259 3472 614
Size: 645 2213 3594
Fragments arranged by size:
3594 2213 645
ZraI GAC'GTC
Cuts at: 3444 3444
Size: 6452
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AgeI AloI ApaI AscI AvrII BaeI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BstEII BstZ17I DraIII
EcoRV FseI FspAI HpaI NcoI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PsrI SanDI
SfiI SgrAI SnaBI SrfI SwaI XmnI