FASTA searches a protein or DNA sequence data bank 36.3.4 Apr, 2011 Please cite: W.R. Pearson & D.J. Lipman PNAS (1988) 85:2444-2448 Query: pF1KA0872, 547 aa 1>>>pF1KA0872 547 - 547 aa - 547 aa Library: /omim/omim.rfq.tfa 60827320 residues in 85289 sequences Statistics: Expectation_n fit: rho(ln(x))= 9.0679+/-0.000319; mu= 1.9826+/- 0.020 mean_var=178.3162+/-35.718, 0's: 0 Z-trim(121.9): 9 B-trim: 50 in 1/58 Lambda= 0.096046 statistics sampled from 39284 (39293) to 39284 sequences Algorithm: FASTA (3.7 Nov 2010) [optimized] Parameters: BL50 matrix (15:-5), open/ext: -10/-2 ktup: 2, E-join: 1 (0.774), E-opt: 0.2 (0.461), width: 16 Scan time: 12.770 The best scores are: opt bits E(85289) NP_055755 (OMIM: 608954) vacuolar fusion protein M ( 547) 3632 515.2 2.1e-145 NP_001273569 (OMIM: 608954) vacuolar fusion protei ( 401) 2564 367.2 5.5e-101 NP_001273568 (OMIM: 608954) vacuolar fusion protei ( 438) 2562 366.9 7.2e-101 XP_011532462 (OMIM: 611464) PREDICTED: vacuolar fu ( 555) 946 143.0 2.3e-33 XP_006713408 (OMIM: 611464) PREDICTED: vacuolar fu ( 555) 946 143.0 2.3e-33 NP_115731 (OMIM: 611464) vacuolar fusion protein M ( 652) 946 143.1 2.6e-33 NP_001135973 (OMIM: 611464) vacuolar fusion protei ( 490) 473 77.5 1.1e-13 >>NP_055755 (OMIM: 608954) vacuolar fusion protein MON1 (547 aa) initn: 3632 init1: 3632 opt: 3632 Z-score: 2731.6 bits: 515.2 E(85289): 2.1e-145 Smith-Waterman score: 3632; 100.0% identity (100.0% similar) in 547 aa overlap (1-547:1-547) 10 20 30 40 50 60 pF1KA0 MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEETGSKDKDQPPSP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEETGSKDKDQPPSP 10 20 30 40 50 60 70 80 90 100 110 120 pF1KA0 SPPPQSEALSSTSRLWSPAAPENSPTCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 SPPPQSEALSSTSRLWSPAAPENSPTCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGK 70 80 90 100 110 120 130 140 150 160 170 180 pF1KA0 PIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 PIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTS 130 140 150 160 170 180 190 200 210 220 230 240 pF1KA0 QSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 QSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDP 190 200 210 220 230 240 250 260 270 280 290 300 pF1KA0 GALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 GALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLD 250 260 270 280 290 300 310 320 330 340 350 360 pF1KA0 PADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 PADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAF 310 320 330 340 350 360 370 380 390 400 410 420 pF1KA0 HAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 HAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDI 370 380 390 400 410 420 430 440 450 460 470 480 pF1KA0 PDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 PDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLA 430 440 450 460 470 480 490 500 510 520 530 540 pF1KA0 WVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_055 WVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAH 490 500 510 520 530 540 pF1KA0 NGLFTGL ::::::: NP_055 NGLFTGL >>NP_001273569 (OMIM: 608954) vacuolar fusion protein MO (401 aa) initn: 2564 init1: 2564 opt: 2564 Z-score: 1933.9 bits: 367.2 E(85289): 5.5e-101 Smith-Waterman score: 2564; 99.2% identity (99.7% similar) in 392 aa overlap (156-547:10-401) 130 140 150 160 170 180 pF1KA0 YGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTSQSAAQ . .::::::::::::::::::::::::::: NP_001 MVSGQLRFGVKTEDHKLVFLQQGPLLLVAMSRTSQSAAQ 10 20 30 190 200 210 220 230 240 pF1KA0 LRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 LRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLL 40 50 60 70 80 90 250 260 270 280 290 300 pF1KA0 GAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 GAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQ 100 110 120 130 140 150 310 320 330 340 350 360 pF1KA0 LLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 LLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAFHAMAA 160 170 180 190 200 210 370 380 390 400 410 420 pF1KA0 CRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 CRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHR 220 230 240 250 260 270 430 440 450 460 470 480 pF1KA0 QLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 QLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSK 280 290 300 310 320 330 490 500 510 520 530 540 pF1KA0 FELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 FELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFT 340 350 360 370 380 390 pF1KA0 GL :: NP_001 GL 400 >>NP_001273568 (OMIM: 608954) vacuolar fusion protein MO (438 aa) initn: 2886 init1: 2562 opt: 2562 Z-score: 1931.9 bits: 366.9 E(85289): 7.2e-101 Smith-Waterman score: 2672; 80.1% identity (80.1% similar) in 547 aa overlap (1-547:1-438) 10 20 30 40 50 60 pF1KA0 MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEETGSKDKDQPPSP ::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 MEVGGDTAAPAPGGAEDLEDTQFPSEEAREGGGVHAVPPDPEDEGLEET----------- 10 20 30 40 70 80 90 100 110 120 pF1KA0 SPPPQSEALSSTSRLWSPAAPENSPTCSPESSSGGQGGDPSDEEWRSQRKHVFVLSEAGK NP_001 ------------------------------------------------------------ 130 140 150 160 170 180 pF1KA0 PIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTS :::::::::::::::::::::: NP_001 --------------------------------------EDHKLVFLQQGPLLLVAMSRTS 50 60 70 190 200 210 220 230 240 pF1KA0 QSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 QSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDP 80 90 100 110 120 130 250 260 270 280 290 300 pF1KA0 GALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 GALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLD 140 150 160 170 180 190 310 320 330 340 350 360 pF1KA0 PADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PADLQLLLDWVGAPAFAAGEAWAPVCLPRFNPDGFFYAYVARLDAMPVCLLLLGTQREAF 200 210 220 230 240 250 370 380 390 400 410 420 pF1KA0 HAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 HAMAACRRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDI 260 270 280 290 300 310 430 440 450 460 470 480 pF1KA0 PDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 PDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLA 320 330 340 350 360 370 490 500 510 520 530 540 pF1KA0 WVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: NP_001 WVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAH 380 390 400 410 420 430 pF1KA0 NGLFTGL ::::::: NP_001 NGLFTGL >>XP_011532462 (OMIM: 611464) PREDICTED: vacuolar fusion (555 aa) initn: 1262 init1: 548 opt: 946 Z-score: 720.1 bits: 143.0 E(85289): 2.3e-33 Smith-Waterman score: 1390; 46.3% identity (70.6% similar) in 521 aa overlap (16-527:58-555) 10 20 30 40 pF1KA0 MEVGGDTAAPAPGGAEDL---EDTQFPSEEAREGGGVHAVPPDPE ::: :: .. .:: ... :: : XP_011 RAESPTPGMAQGMEPGAGQEGAMFVHARSYEDLTESEDGAASGDSHKEG--TRGPPPLPT 30 40 50 60 70 80 50 60 70 80 90 100 pF1KA0 D-EGLEETGSKDKDQPPSPSPPPQSEALSSTSRLW-SPAAPENSPTCSPESSSGGQGGDP : . . . :. . : . . : : : ..:. : : . . :. : : :: XP_011 DMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWLEPPGAVGRPATEPPRE-GTTEGDE 90 100 110 120 130 140 110 120 130 140 150 pF1KA0 SD--EEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYA : : :: ..::::::::::::.:::::: ::::.:::::.:::::... .:::.:.: XP_011 EDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHA 150 160 170 180 190 200 160 170 180 190 200 210 pF1KA0 EDHKLVFLQQGPLLLVAMSRTSQSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDL . .:.::....::.:::..:: ::: .: ::: .. ::.: :: :....:: .:::::: XP_011 DGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDL 210 220 230 240 250 260 220 230 240 250 260 270 pF1KA0 RRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVL ::::.:::: : ::. : .:: ..:.::.::.::: .::...: :.. : .:..:.: XP_011 RRLLSGSERITDNLLQLMARDP-SFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSIL 270 280 290 300 310 320 280 290 300 310 320 330 pF1KA0 AVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPA-FAAGEAWAPVCLPRFNPDGFFY . ..:.. ..... . : : ::.::.. ... . : ::::.:::::.:: :::. XP_011 LARNQLVALVRRKDQF----LHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFH 330 340 350 360 370 340 350 360 370 380 390 pF1KA0 AYVARLDA-MPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSAS :... :. .::::..:.:: : :.. ::: .. .. :: :: :: XP_011 AHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALR-------- 380 390 400 410 420 430 400 410 420 430 440 450 pF1KA0 APAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLH .: ::: :: : ::::::: .. :::::.::::. :::..:: ::. :: XP_011 TPYYSVAQVGIPDLRHFLYK-------SKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLH 440 450 460 470 480 460 470 480 490 500 510 pF1KA0 ARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEE .: :..::::. ::... .:.::::::. :::: : ::: :::.:. .. ::.::..::: XP_011 SRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEE 490 500 510 520 530 540 520 530 540 pF1KA0 DRLFIRYPPKYSTPPATSTDQAAHNGLFTGL ::::: : : XP_011 DRLFILTPLTY 550 >>XP_006713408 (OMIM: 611464) PREDICTED: vacuolar fusion (555 aa) initn: 1262 init1: 548 opt: 946 Z-score: 720.1 bits: 143.0 E(85289): 2.3e-33 Smith-Waterman score: 1390; 46.3% identity (70.6% similar) in 521 aa overlap (16-527:58-555) 10 20 30 40 pF1KA0 MEVGGDTAAPAPGGAEDL---EDTQFPSEEAREGGGVHAVPPDPE ::: :: .. .:: ... :: : XP_006 RAESPTPGMAQGMEPGAGQEGAMFVHARSYEDLTESEDGAASGDSHKEG--TRGPPPLPT 30 40 50 60 70 80 50 60 70 80 90 100 pF1KA0 D-EGLEETGSKDKDQPPSPSPPPQSEALSSTSRLW-SPAAPENSPTCSPESSSGGQGGDP : . . . :. . : . . : : : ..:. : : . . :. : : :: XP_006 DMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWLEPPGAVGRPATEPPRE-GTTEGDE 90 100 110 120 130 140 110 120 130 140 150 pF1KA0 SD--EEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYA : : :: ..::::::::::::.:::::: ::::.:::::.:::::... .:::.:.: XP_006 EDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHA 150 160 170 180 190 200 160 170 180 190 200 210 pF1KA0 EDHKLVFLQQGPLLLVAMSRTSQSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDL . .:.::....::.:::..:: ::: .: ::: .. ::.: :: :....:: .:::::: XP_006 DGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDL 210 220 230 240 250 260 220 230 240 250 260 270 pF1KA0 RRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVL ::::.:::: : ::. : .:: ..:.::.::.::: .::...: :.. : .:..:.: XP_006 RRLLSGSERITDNLLQLMARDP-SFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSIL 270 280 290 300 310 320 280 290 300 310 320 330 pF1KA0 AVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPA-FAAGEAWAPVCLPRFNPDGFFY . ..:.. ..... . : : ::.::.. ... . : ::::.:::::.:: :::. XP_006 LARNQLVALVRRKDQF----LHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFH 330 340 350 360 370 340 350 360 370 380 390 pF1KA0 AYVARLDA-MPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSAS :... :. .::::..:.:: : :.. ::: .. .. :: :: :: XP_006 AHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALR-------- 380 390 400 410 420 430 400 410 420 430 440 450 pF1KA0 APAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLH .: ::: :: : ::::::: .. :::::.::::. :::..:: ::. :: XP_006 TPYYSVAQVGIPDLRHFLYK-------SKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLH 440 450 460 470 480 460 470 480 490 500 510 pF1KA0 ARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEE .: :..::::. ::... .:.::::::. :::: : ::: :::.:. .. ::.::..::: XP_006 SRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEE 490 500 510 520 530 540 520 530 540 pF1KA0 DRLFIRYPPKYSTPPATSTDQAAHNGLFTGL ::::: : : XP_006 DRLFILTPLTY 550 >>NP_115731 (OMIM: 611464) vacuolar fusion protein MON1 (652 aa) initn: 1262 init1: 548 opt: 946 Z-score: 719.0 bits: 143.1 E(85289): 2.6e-33 Smith-Waterman score: 1390; 46.3% identity (70.6% similar) in 521 aa overlap (16-527:155-652) 10 20 30 40 pF1KA0 MEVGGDTAAPAPGGAEDL---EDTQFPSEEAREGGGVHAVPPDPE ::: :: .. .:: ... :: : NP_115 RAESPTPGMAQGMEPGAGQEGAMFVHARSYEDLTESEDGAASGDSHKEG--TRGPPPLPT 130 140 150 160 170 180 50 60 70 80 90 100 pF1KA0 D-EGLEETGSKDKDQPPSPSPPPQSEALSSTSRLW-SPAAPENSPTCSPESSSGGQGGDP : . . . :. . : . . : : : ..:. : : . . :. : : :: NP_115 DMRQISQDFSELSTQLTGVARDLQEEMLPGSSEDWLEPPGAVGRPATEPPRE-GTTEGDE 190 200 210 220 230 240 110 120 130 140 150 pF1KA0 SD--EEWRSQRKHVFVLSEAGKPIYSRYGSVEALSATMGVMTALVSFVQSAGDAIRAIYA : : :: ..::::::::::::.:::::: ::::.:::::.:::::... .:::.:.: NP_115 EDATEAWRLHQKHVFVLSEAGKPVYSRYGSEEALSSTMGVMVALVSFLEADKNAIRSIHA 250 260 270 280 290 300 160 170 180 190 200 210 pF1KA0 EDHKLVFLQQGPLLLVAMSRTSQSAAQLRGELLAVHAQIVSTLTRASVARIFAHKQNYDL . .:.::....::.:::..:: ::: .: ::: .. ::.: :: :....:: .:::::: NP_115 DGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDL 310 320 330 340 350 360 220 230 240 250 260 270 pF1KA0 RRLLAGSERTLDRLLDSMEQDPGALLLGAVRCVPLARPLRDALGALLRRCTAPGLALSVL ::::.:::: : ::. : .:: ..:.::.::.::: .::...: :.. : .:..:.: NP_115 RRLLSGSERITDNLLQLMARDP-SFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSIL 370 380 390 400 410 420 280 290 300 310 320 330 pF1KA0 AVGGRLITAAQERNVLAECRLDPADLQLLLDWVGAPA-FAAGEAWAPVCLPRFNPDGFFY . ..:.. ..... . : : ::.::.. ... . : ::::.:::::.:: :::. NP_115 LARNQLVALVRRKDQF----LHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFH 430 440 450 460 470 340 350 360 370 380 390 pF1KA0 AYVARLDA-MPVCLLLLGTQREAFHAMAACRRLVEDGMHALGAMRALGEAASFSNASSAS :... :. .::::..:.:: : :.. ::: .. .. :: :: :: NP_115 AHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALR-------- 480 490 500 510 520 400 410 420 430 440 450 pF1KA0 APAYSVQAVGAPGLRHFLYKPLDIPDHHRQLPQFTSPELEAPYSREEERQRLSDLYHRLH .: ::: :: : ::::::: .. :::::.::::. :::..:: ::. :: NP_115 TPYYSVAQVGIPDLRHFLYK-------SKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLH 530 540 550 560 570 580 460 470 480 490 500 510 pF1KA0 ARLHSTSRPLRLIYHVAEKETLLAWVTSKFELYTCLSPLVTKAGAILVVTKLLRWVKKEE .: :..::::. ::... .:.::::::. :::: : ::: :::.:. .. ::.::..::: NP_115 SRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEE 590 600 610 620 630 640 520 530 540 pF1KA0 DRLFIRYPPKYSTPPATSTDQAAHNGLFTGL ::::: : : NP_115 DRLFILTPLTY 650 >>NP_001135973 (OMIM: 611464) vacuolar fusion protein MO (490 aa) initn: 964 init1: 400 opt: 473 Z-score: 366.7 bits: 77.5 E(85289): 1.1e-13 Smith-Waterman score: 1073; 47.4% identity (73.6% similar) in 371 aa overlap (159-527:140-490) 130 140 150 160 170 180 pF1KA0 VEALSATMGVMTALVSFVQSAGDAIRAIYAEDHKLVFLQQGPLLLVAMSRTSQSAAQLRG . .:.::....::.:::..:: ::: .: NP_001 CLDGTLTPSDGQSMERAESPTPGMAQGMEPDGYKVVFVRRSPLVLVAVARTRQSAQELAQ 110 120 130 140 150 160 190 200 210 220 230 240 pF1KA0 ELLAVHAQIVSTLTRASVARIFAHKQNYDLRRLLAGSERTLDRLLDSMEQDPGALLLGAV ::: .. ::.: :: :....:: .::::::::::.:::: : ::. : .:: ..:.::. NP_001 ELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLLSGSERITDNLLQLMARDP-SFLMGAA 170 180 190 200 210 220 250 260 270 280 290 300 pF1KA0 RCVPLARPLRDALGALLRRCTAPGLALSVLAVGGRLITAAQERNVLAECRLDPADLQLLL ::.::: .::...: :.. : .:..:.: . ..:.. ..... . : : ::.::. NP_001 RCLPLAAAVRDTVSASLQQARARSLVFSILLARNQLVALVRRKDQF----LHPIDLHLLF 230 240 250 260 270 280 310 320 330 340 350 360 pF1KA0 DWVGAPA-FAAGEAWAPVCLPRFNPDGFFYAYVARLDA-MPVCLLLLGTQREAFHAMAAC . ... . : ::::.:::::.:: :::.:... :. .::::..:.:: : :.. : NP_001 NLISSSSSFREGEAWTPVCLPKFNAAGFFHAHISYLEPDTDLCLLLVSTDREDFFAVSDC 290 300 310 320 330 340 370 380 390 400 410 420 pF1KA0 RRLVEDGMHALGAMRALGEAASFSNASSASAPAYSVQAVGAPGLRHFLYKPLDIPDHHRQ :: .. .. :: :: :: .: ::: :: : ::::::: .. NP_001 RRRFQERLRKRGAHLALREALR--------TPYYSVAQVGIPDLRHFLYKS-------KS 350 360 370 380 430 440 450 460 470 480 pF1KA0 LPQFTSPELEAPYSREEERQRLSDLYHRLHARLHSTSRPLRLIYHVAEKETLLAWVTSKF :::::.::::. :::..:: ::. ::.: :..::::. ::... .:.::::::. : NP_001 SGLFTSPEIEAPYTSEEEQERLLGLYQYLHSRAHNASRPLKTIYYTGPNENLLAWVTGAF 390 400 410 420 430 440 490 500 510 520 530 540 pF1KA0 ELYTCLSPLVTKAGAILVVTKLLRWVKKEEDRLFIRYPPKYSTPPATSTDQAAHNGLFTG ::: : ::: :::.:. .. ::.::..:::::::: : : NP_001 ELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFILTPLTY 450 460 470 480 490 pF1KA0 L 547 residues in 1 query sequences 60827320 residues in 85289 library sequences Tcomplib [36.3.4 Apr, 2011] (8 proc) start: Fri Nov 4 01:05:42 2016 done: Fri Nov 4 01:05:44 2016 Total Scan time: 12.770 Total Display time: 0.050 Function used was FASTA [36.3.4 Apr, 2011]