Restriction map for
pF1KA0872
(Circular) (Six-base) MAPSORT of: pF1KA0872.seq Check: 7554 from: 1 to: 4754
pF1KA0872 4754 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1594 1594
Size: 4754
AccI GT'mk_AC
Cuts at: 954 1792 954
Size: 838 3916
Acc65I G'GTAC_C
Cuts at: 1770 1770
Size: 4754
AclI AA'CG_TT
Cuts at: 4594 4594
Size: 4754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 3011 3364 4026 2579
Size: 432 353 662 3307
Fragments arranged by size:
3307 662 432 353
AflIII A'CryG_T
Cuts at: 364 712 2106 3906 4187 364
Size: 348 1394 1800 281 931
Fragments arranged by size:
1800 1394 931 348 281
AleI CACnn'nnGTG
Cuts at: 1080 1080
Size: 4754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1098 1098
Size: 4754
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 157 189 157
Size: 32 4722
AlwNI CAG_nnn'CTG
Cuts at: 349 919 1026 1449 2059 3497 349
Size: 570 107 423 610 1438 1606
Fragments arranged by size:
1606 1438 610 570 423 107
ApaI G_GGCC'C
Cuts at: 616 616
Size: 4754
ApaLI G'TGCA_C
Cuts at: 685 1043 3592 685
Size: 358 2549 1847
Fragments arranged by size:
2549 1847 358
ApoI r'AATT_y
Cuts at: 1561 1758 4107 4363 1561
Size: 197 2349 256 1952
Fragments arranged by size:
2349 1952 256 197
AseI AT'TA_AT
Cuts at: 20 20
Size: 4754
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
AvaI C'yCGr_G
Cuts at: 142 1774 142
Size: 1632 3122
BamHI G'GATC_C
Cuts at: 225 256 1779 225
Size: 31 1523 3200
Fragments arranged by size:
3200 1523 31
BanI G'GyrC_C
Cuts at: 844 1074 1168 1208 1240 1326 1770 2184 2432
Size: 230 94 40 32 86 444 414 248
Cuts at: 2432 2467 844
Size: 35 3131
Fragments arranged by size:
3131 444 414 248 230 94 86 40
35 32
BanII G_rGCy'C
Cuts at: 616 834 1571 1768 2798 616
Size: 218 737 197 1030 2572
Fragments arranged by size:
2572 1030 737 218 197
BbeI G_GCGC'C
Cuts at: 1212 1330 2188 2436 1212
Size: 118 858 248 3530
Fragments arranged by size:
3530 858 248 118
BbsI GAAGACnn'nnnn_
Cuts at: 239 239
Size: 4754
BbvCI CC'TCA_GC
Cuts at: 581 1582 581
Size: 1001 3753
BciVI GTATCCnnnnn_n'
Cuts at: 2652 3708 4754 2652
Size: 1056 1046 2652
Fragments arranged by size:
2652 1056 1046
BclI T'GATC_A
Cuts at: 2274 2274
Size: 4754
BfrBI ATG'CAT
Cuts at: 1230 1230
Size: 4754
BglI GCCn_nnn'nGGC
Cuts at: 1057 1126 1057
Size: 69 4685
BglII A'GATC_T
Cuts at: 2269 4306 2269
Size: 2037 2717
BlpI GC'TnA_GC
Cuts at: 1866 1866
Size: 4754
Bme1580I G_kGCm'C
Cuts at: 616 689 863 1047 1077 1254 2379 2472 3596
Size: 73 174 184 30 177 1125 93 1124
Cuts at: 3596 616
Size: 1774
Fragments arranged by size:
1774 1125 1124 184 177 174 93 73
30
BmrI ACTGGGnnnn_n'
Cuts at: 173 294 401 1044 1386 1966 2047 2382 4526
Size: 121 107 643 342 580 81 335 2144
Cuts at: 4526 173
Size: 401
Fragments arranged by size:
2144 643 580 401 342 335 121 107
81
BmtI G_CTAG'C
Cuts at: 682 682
Size: 4754
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 399 853 3121 399
Size: 454 2268 2032
Fragments arranged by size:
2268 2032 454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 354 407 265
Size: 89 53 4612
Fragments arranged by size:
4612 89 53
Bpu10I CC'TnA_GC
Cuts at: 581 1406 1582 581
Size: 825 176 3753
Fragments arranged by size:
3753 825 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1930 2809 3523 3821 1930
Size: 879 714 298 2863
Fragments arranged by size:
2863 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4458 4458
Size: 4754
BsaAI yAC'GTr
Cuts at: 715 1125 1521 2107 715
Size: 410 396 586 3362
Fragments arranged by size:
3362 586 410 396
BsaBI GATnn'nnATC
Cuts at: 6 2268 4305 6
Size: 2262 2037 455
Fragments arranged by size:
2262 2037 455
BsaHI Gr'CG_yC
Cuts at: 1209 1327 2185 2433 3135 4631 1209
Size: 118 858 248 702 1496 1332
Fragments arranged by size:
1496 1332 858 702 248 118
BsaWI w'CCGG_w
Cuts at: 1943 2464 3142 3553 3700 3974 1943
Size: 521 678 411 147 274 2723
Fragments arranged by size:
2723 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 487 517 1477 1507 487
Size: 30 960 30 3734
Fragments arranged by size:
3734 960 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 134 430 889 1447 1450 1666 134
Size: 296 459 558 3 216 3222
Fragments arranged by size:
3222 558 459 296 216 3
BseYI C'CCAG_C
Cuts at: 280 659 1008 1637 2179 2937 3602 4010 280
Size: 379 349 629 542 758 665 408 1024
Fragments arranged by size:
1024 758 665 629 542 408 379 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1333 565
Size: 768 3986
BsiEI CG_ry'CG
Cuts at: 104 954 2296 2342 3572 104
Size: 850 1342 46 1230 1286
Fragments arranged by size:
1342 1286 1230 850 46
BsiHKAI G_wGCw'C
Cuts at: 689 706 1047 1299 1571 1768 2546 2736 3596
Size: 17 341 252 272 197 778 190 860
Cuts at: 3596 689
Size: 1847
Fragments arranged by size:
1847 860 778 341 272 252 197 190
17
BsmBI CGTCTCn'nnnn_
Cuts at: 875 1486 875
Size: 611 4143
Bsp1286I G_dGCh'C
Cuts at: 616 689 706 834 863 1047 1077 1254 1299
Size: 73 17 128 29 184 30 177 45
Cuts at: 1299 1571 1768 2379 2472 2546 2736 2798 3596
Size: 272 197 611 93 74 190 62 798
Cuts at: 3596 616
Size: 1774
Fragments arranged by size:
1774 798 611 272 197 190 184 177
128 93 74 73 62 45 30 29
17
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4754
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1025 1585 1594 1804 2018 2320 2701 1025
Size: 560 9 210 214 302 381 3078
Fragments arranged by size:
3078 560 381 302 214 210 9
BsrBI CCG'CTC
Cuts at: 50 978 1440 3046 3100 4584 4749 50
Size: 928 462 1606 54 1484 165 55
Fragments arranged by size:
1606 1484 928 462 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2666 2666
Size: 4754
BsrFI r'CCGG_y
Cuts at: 998 1205 1658 2752 2933 998
Size: 207 453 1094 181 2819
Fragments arranged by size:
2819 1094 453 207 181
BssSI C'ACGA_G
Cuts at: 625 3025 3733 625
Size: 2400 708 1646
Fragments arranged by size:
2400 1646 708
BstAPI GCAn_nnn'nTGC
Cuts at: 892 1026 2131 892
Size: 134 1105 3515
Fragments arranged by size:
3515 1105 134
BstBI TT'CG_AA
Cuts at: 3115 3115
Size: 4754
BstEII G'GTnAC_C
Cuts at: 1551 1593 1551
Size: 42 4712
BstYI r'GATC_y
Cuts at: 1 225 256 1779 2246 2269 2604 2850 3156
Size: 224 31 1523 467 23 335 246 306
Cuts at: 3156 3168 3254 4306 1
Size: 12 86 1052 449
Fragments arranged by size:
1523 1052 467 449 335 306 246 224
86 31 23 12
BtgI C'CryG_G
Cuts at: 107 512 1193 107
Size: 405 681 3668
Fragments arranged by size:
3668 681 405
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2704 2885 3048 2704
Size: 181 163 4410
Fragments arranged by size:
4410 181 163
BtsI GCAGTG_nn'
Cuts at: 125 1997 125
Size: 1872 2882
DraI TTT'AAA
Cuts at: 1753 1753
Size: 4754
DraIII CAC_nnn'GTG
Cuts at: 892 892
Size: 4754
DrdI GACnn_nn'nnGTC
Cuts at: 648 2460 3804 648
Size: 1812 1344 1598
Fragments arranged by size:
1812 1598 1344
EaeI y'GGCC_r
Cuts at: 629 989 1196 2339 2513 2904 2931 4276 629
Size: 360 207 1143 174 391 27 1345 1107
Fragments arranged by size:
1345 1143 1107 391 360 207 174 27
EagI C'GGCC_G
Cuts at: 2339 2339
Size: 4754
EarI CTCTTCn'nnn_
Cuts at: 1669 2777 2987 1669
Size: 1108 210 3436
Fragments arranged by size:
3436 1108 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 751 2962 3704 3850 4564 751
Size: 2211 742 146 714 941
Fragments arranged by size:
2211 941 742 714 146
EcoICRI GAG'CTC
Cuts at: 1569 1766 1569
Size: 197 4557
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 354 407 2579 3011 3364 4026 265
Size: 89 53 2172 432 353 662 993
Fragments arranged by size:
2172 993 662 432 353 89 53
EcoNI CCTnn'n_nnAGG
Cuts at: 607 1258 607
Size: 651 4103
EcoO109I rG'GnC_Cy
Cuts at: 240 309 402 612 822 1416 1893 240
Size: 69 93 210 210 594 477 3101
Fragments arranged by size:
3101 594 477 210 210 93 69
EcoRI G'AATT_C
Cuts at: 1758 1758
Size: 4754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4754
FspI TGC'GCA
Cuts at: 2535 2535
Size: 4754
HaeII r_GCGC'y
Cuts at: 502 919 931 1212 1330 2188 2436 3666 4437
Size: 417 12 281 118 858 248 1230 771
Cuts at: 4437 502
Size: 819
Fragments arranged by size:
1230 858 819 771 417 281 248 118
12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 298 330 487 519 548 580 2596 2626 2628
Size: 32 157 32 29 32 2016 30 2
Cuts at: 2628 2658 298
Size: 30 2394
Fragments arranged by size:
2394 2016 157 32 32 32 30 30
29 2
HincII GTy'rAC
Cuts at: 955 1793 955
Size: 838 3916
KasI G'GCGC_C
Cuts at: 1208 1326 2184 2432 1208
Size: 118 858 248 3530
Fragments arranged by size:
3530 858 248 118
KpnI G_GTAC'C
Cuts at: 1774 1774
Size: 4754
MluI A'CGCG_T
Cuts at: 4187 4187
Size: 4754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 96 927 1433 3513 3697 96
Size: 831 506 2080 184 1153
Fragments arranged by size:
2080 1153 831 506 184
MscI TGG'CCA
Cuts at: 631 2515 631
Size: 1884 2870
MslI CAynn'nnrTG
Cuts at: 1080 2743 2870 1080
Size: 1663 127 2964
Fragments arranged by size:
2964 1663 127
MspA1I CmG'CkG
Cuts at: 432 663 876 1012 1359 1446 1865 2179 2539
Size: 231 213 136 347 87 419 314 360
Cuts at: 2539 3323 3568 432
Size: 784 245 1618
Fragments arranged by size:
1618 784 419 360 347 314 245 231
213 136 87
NaeI GCC'GGC
Cuts at: 1000 1207 2935 1000
Size: 207 1728 2819
Fragments arranged by size:
2819 1728 207
NarI GG'CG_CC
Cuts at: 1209 1327 2185 2433 1209
Size: 118 858 248 3530
Fragments arranged by size:
3530 858 248 118
NcoI C'CATG_G
Cuts at: 107 512 1193 107
Size: 405 681 3668
Fragments arranged by size:
3668 681 405
NgoMIV G'CCGG_C
Cuts at: 998 1205 2933 998
Size: 207 1728 2819
Fragments arranged by size:
2819 1728 207
NheI G'CTAG_C
Cuts at: 678 678
Size: 4754
NsiI A_TGCA'T
Cuts at: 1232 1232
Size: 4754
NspI r_CATG'y
Cuts at: 368 442 1234 1477 1807 2307 3910 368
Size: 74 792 243 330 500 1603 1212
Fragments arranged by size:
1603 1212 792 500 330 243 74
PciI A'CATG_T
Cuts at: 364 3906 364
Size: 3542 1212
PflMI CCAn_nnn'nTGG
Cuts at: 594 1194 594
Size: 600 4154
PmeI GTTT'AAAC
Cuts at: 1753 1753
Size: 4754
PmlI CAC'GTG
Cuts at: 715 1521 715
Size: 806 3948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4722
PpuMI rG'GwC_Cy
Cuts at: 402 822 402
Size: 420 4334
PsiI TTA'TAA
Cuts at: 961 961
Size: 4754
PspOMI G'GGCC_C
Cuts at: 612 612
Size: 4754
PstI C_TGCA'G
Cuts at: 345 1022 1801 2486 345
Size: 677 779 685 2613
Fragments arranged by size:
2613 779 685 677
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4754
PvuII CAG'CTG
Cuts at: 663 1012 2179 2539 663
Size: 349 1167 360 2878
Fragments arranged by size:
2878 1167 360 349
RsrII CG'GwC_CG
Cuts at: 2949 2949
Size: 4754
SacI G_AGCT'C
Cuts at: 1571 1768 1571
Size: 197 4557
SalI G'TCGA_C
Cuts at: 953 1791 953
Size: 838 3916
SanDI GG'GwC_CC
Cuts at: 402 402
Size: 4754
SapI GCTCTTCn'nnn_
Cuts at: 1669 2777 2987 1669
Size: 1108 210 3436
Fragments arranged by size:
3436 1108 210
SbfI CC_TGCA'GG
Cuts at: 1801 1801
Size: 4754
ScaI AGT'ACT
Cuts at: 1687 4271 1687
Size: 2584 2170
SfcI C'TryA_G
Cuts at: 33 341 1018 1305 1422 1797 2482 3450 3641
Size: 308 677 287 117 375 685 968 191
Cuts at: 3641 33
Size: 1146
Fragments arranged by size:
1146 968 685 677 375 308 287 191
117
SfoI GGC'GCC
Cuts at: 1210 1328 2186 2434 1210
Size: 118 858 248 3530
Fragments arranged by size:
3530 858 248 118
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
SmaI CCC'GGG
Cuts at: 144 1776 144
Size: 1632 3122
SmlI C'TyrA_G
Cuts at: 1909 2824 3538 3800 1909
Size: 915 714 262 2863
Fragments arranged by size:
2863 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4754
SphI G_CATG'C
Cuts at: 1234 1477 1807 2307 1234
Size: 243 330 500 3681
Fragments arranged by size:
3681 500 330 243
StyI C'CwwG_G
Cuts at: 107 260 512 1193 1236 1254 1598 1888 2242
Size: 153 252 681 43 18 344 290 354
Cuts at: 2242 107
Size: 2619
Fragments arranged by size:
2619 681 354 344 290 252 153 43
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 505 812 832 1026 1030 1186 1539 1629
Size: 211 307 20 194 4 156 353 90
Cuts at: 1629 1695 2337 2470 2929 3138 294
Size: 66 642 133 459 209 1910
Fragments arranged by size:
1910 642 459 353 307 211 209 194
156 133 90 66 20 4
TatI w'GTAC_w
Cuts at: 1685 4269 1685
Size: 2584 2170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1477 1509 4271 4303 1477
Size: 32 2762 32 1928
Fragments arranged by size:
2762 1928 32 32
Tth111I GACn'n_nGTC
Cuts at: 2551 2551
Size: 4754
XbaI T'CTAG_A
Cuts at: 1785 1785
Size: 4754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2249 2249
Size: 4754
XmaI C'CCGG_G
Cuts at: 142 1774 142
Size: 1632 3122
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NsiI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AscI AvrII BaeI
BcgI BmgBI BsiWI BsmI BspEI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI PacI PasI PfoI
PshAI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI