Restriction map for
pF1KA0877
(Circular) (Six-base) MAPSORT of: pF1KA0877.seq Check: 2544 from: 1 to: 4769
pF1KA0877 4769 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 904 1502 1807 904
Size: 598 305 3866
Fragments arranged by size:
3866 598 305
Acc65I G'GTAC_C
Cuts at: 1785 1785
Size: 4769
AclI AA'CG_TT
Cuts at: 4609 4609
Size: 4769
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2594 3026 3379 4041 388
Size: 2206 432 353 662 1116
Fragments arranged by size:
2206 1116 662 432 353
AflIII A'CryG_T
Cuts at: 1056 1172 2121 3921 4202 1056
Size: 116 949 1800 281 1623
Fragments arranged by size:
1800 1623 949 281 116
AleI CACnn'nnGTG
Cuts at: 1173 1175 1173
Size: 2 4767
AlwNI CAG_nnn'CTG
Cuts at: 2074 3512 2074
Size: 1438 3331
ApaLI G'TGCA_C
Cuts at: 3607 3607
Size: 4769
ApoI r'AATT_y
Cuts at: 818 868 1631 1773 4122 4378 818
Size: 50 763 142 2349 256 1209
Fragments arranged by size:
2349 1209 763 256 142 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4769
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
AvaI C'yCGr_G
Cuts at: 1789 1789
Size: 4769
BamHI G'GATC_C
Cuts at: 1794 1794
Size: 4769
BanI G'GyrC_C
Cuts at: 1399 1785 2199 2447 2482 1399
Size: 386 414 248 35 3686
Fragments arranged by size:
3686 414 386 248 35
BanII G_rGCy'C
Cuts at: 1783 2813 1783
Size: 1030 3739
BbeI G_GCGC'C
Cuts at: 2203 2451 2203
Size: 248 4521
BbsI GAAGACnn'nnnn_
Cuts at: 1475 1475
Size: 4769
BciVI GTATCCnnnnn_n'
Cuts at: 2667 3723 4769 2667
Size: 1056 1046 2667
Fragments arranged by size:
2667 1056 1046
BclI T'GATC_A
Cuts at: 1077 2289 1077
Size: 1212 3557
BfrBI ATG'CAT
Cuts at: 1102 1102
Size: 4769
BglII A'GATC_T
Cuts at: 2284 4321 2284
Size: 2037 2732
BlpI GC'TnA_GC
Cuts at: 1881 1881
Size: 4769
Bme1580I G_kGCm'C
Cuts at: 2394 2487 3611 2394
Size: 93 1124 3552
Fragments arranged by size:
3552 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1299 1981 2062 2397 4541 1299
Size: 682 81 335 2144 1527
Fragments arranged by size:
2144 1527 682 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 181 3136 181
Size: 2955 1814
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1185 1500 1945 2824 3538 3836 1185
Size: 315 445 879 714 298 2118
Fragments arranged by size:
2118 879 714 445 315 298
BsaI GGTCTCn'nnnn_
Cuts at: 4473 4473
Size: 4769
BsaAI yAC'GTr
Cuts at: 2122 2122
Size: 4769
BsaBI GATnn'nnATC
Cuts at: 6 2283 4320 6
Size: 2277 2037 455
Fragments arranged by size:
2277 2037 455
BsaHI Gr'CG_yC
Cuts at: 2200 2448 3150 4646 2200
Size: 248 702 1496 2323
Fragments arranged by size:
2323 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1958 2479 3157 3568 3715 3989 1958
Size: 521 678 411 147 274 2738
Fragments arranged by size:
2738 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 290 320 353 383 1076 1106 290
Size: 30 33 30 693 30 3953
Fragments arranged by size:
3953 693 33 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 170 170
Size: 4769
BseYI C'CCAG_C
Cuts at: 1660 2194 2952 3617 4025 1660
Size: 534 758 665 408 2404
Fragments arranged by size:
2404 758 665 534 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 1418 932
Size: 486 4283
BsiEI CG_ry'CG
Cuts at: 104 2311 2357 3587 104
Size: 2207 46 1230 1286
Fragments arranged by size:
2207 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1783 2561 2751 3611 1783
Size: 778 190 860 2941
Fragments arranged by size:
2941 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 1783 2394 2487 2561 2751 2813 3611 1783
Size: 611 93 74 190 62 798 2941
Fragments arranged by size:
2941 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1819 2033 2335 2716 1819
Size: 214 302 381 3872
Fragments arranged by size:
3872 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3061 3115 4599 4764 50
Size: 3011 54 1484 165 55
Fragments arranged by size:
3011 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 716 1102 1277 2681 716
Size: 386 175 1404 2804
Fragments arranged by size:
2804 1404 386 175
BsrFI r'CCGG_y
Cuts at: 2767 2948 2767
Size: 181 4588
BssSI C'ACGA_G
Cuts at: 3040 3748 3040
Size: 708 4061
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 2146 1393
Size: 753 4016
BstBI TT'CG_AA
Cuts at: 3130 3130
Size: 4769
BstEII G'GTnAC_C
Cuts at: 147 147
Size: 4769
BstYI r'GATC_y
Cuts at: 1 198 1603 1647 1794 2261 2284 2619 2865
Size: 197 1405 44 147 467 23 335 246
Cuts at: 2865 3171 3183 3269 4321 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1405 1052 467 449 335 306 246 197
147 86 44 23 12
BstZ17I GTA'TAC
Cuts at: 905 1503 905
Size: 598 4171
BtgI C'CryG_G
Cuts at: 107 107
Size: 4769
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2719 2900 3063 2719
Size: 181 163 4425
Fragments arranged by size:
4425 181 163
BtsI GCAGTG_nn'
Cuts at: 1394 2012 1394
Size: 618 4151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1288 1323 1288
Size: 35 4734
DraI TTT'AAA
Cuts at: 236 1768 236
Size: 1532 3237
DrdI GACnn_nn'nnGTC
Cuts at: 2475 3819 2475
Size: 1344 3425
EaeI y'GGCC_r
Cuts at: 2354 2528 2919 2946 4291 2354
Size: 174 391 27 1345 2832
Fragments arranged by size:
2832 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2354 2354
Size: 4769
EarI CTCTTCn'nnn_
Cuts at: 1162 1577 2792 3002 1162
Size: 415 1215 210 2929
Fragments arranged by size:
2929 1215 415 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2977 3719 3865 4579 2977
Size: 742 146 714 3167
Fragments arranged by size:
3167 742 714 146
EcoICRI GAG'CTC
Cuts at: 1781 1781
Size: 4769
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 2594 3026 3379 4041 388
Size: 2206 432 353 662 1116
Fragments arranged by size:
2206 1116 662 432 353
EcoO109I rG'GnC_Cy
Cuts at: 1908 1908
Size: 4769
EcoRI G'AATT_C
Cuts at: 1773 1773
Size: 4769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1366 2788 1366
Size: 1422 3347
FspI TGC'GCA
Cuts at: 2550 2550
Size: 4769
HaeII r_GCGC'y
Cuts at: 2203 2451 3681 4452 2203
Size: 248 1230 771 2520
Fragments arranged by size:
2520 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 351 383 1139 1171 2611 2641 2643 2673 351
Size: 32 756 32 1440 30 2 30 2447
Fragments arranged by size:
2447 1440 756 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1808 1808
Size: 4769
HindIII A'AGCT_T
Cuts at: 378 456 378
Size: 78 4691
KasI G'GCGC_C
Cuts at: 2199 2447 2199
Size: 248 4521
KpnI G_GTAC'C
Cuts at: 1789 1789
Size: 4769
MfeI C'AATT_G
Cuts at: 1108 1108
Size: 4769
MluI A'CGCG_T
Cuts at: 4202 4202
Size: 4769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 121 1498 3528 3712 121
Size: 1377 2030 184 1178
Fragments arranged by size:
2030 1377 1178 184
MscI TGG'CCA
Cuts at: 2530 2530
Size: 4769
MslI CAynn'nnrTG
Cuts at: 1173 1175 1241 2758 2885 1173
Size: 2 66 1517 127 3057
Fragments arranged by size:
3057 1517 127 66 2
MspA1I CmG'CkG
Cuts at: 916 1880 2194 2554 3338 3583 916
Size: 964 314 360 784 245 2102
Fragments arranged by size:
2102 964 784 360 314 245
NaeI GCC'GGC
Cuts at: 2950 2950
Size: 4769
NarI GG'CG_CC
Cuts at: 2200 2448 2200
Size: 248 4521
NcoI C'CATG_G
Cuts at: 107 107
Size: 4769
NgoMIV G'CCGG_C
Cuts at: 2948 2948
Size: 4769
NsiI A_TGCA'T
Cuts at: 1104 1104
Size: 4769
NspI r_CATG'y
Cuts at: 1060 1176 1822 2322 3925 1060
Size: 116 646 500 1603 1904
Fragments arranged by size:
1904 1603 646 500 116
PciI A'CATG_T
Cuts at: 1056 1172 3921 1056
Size: 116 2749 1904
Fragments arranged by size:
2749 1904 116
PmeI GTTT'AAAC
Cuts at: 1768 1768
Size: 4769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3215 3247 3215
Size: 32 4737
PsiI TTA'TAA
Cuts at: 881 881
Size: 4769
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1720 1752 1720
Size: 32 4737
PstI C_TGCA'G
Cuts at: 1816 2501 1816
Size: 685 4084
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4769
PvuII CAG'CTG
Cuts at: 2194 2554 2194
Size: 360 4409
RsrII CG'GwC_CG
Cuts at: 2964 2964
Size: 4769
SacI G_AGCT'C
Cuts at: 1783 1783
Size: 4769
SalI G'TCGA_C
Cuts at: 1806 1806
Size: 4769
SapI GCTCTTCn'nnn_
Cuts at: 2792 3002 2792
Size: 210 4559
SbfI CC_TGCA'GG
Cuts at: 1816 1816
Size: 4769
ScaI AGT'ACT
Cuts at: 1374 4286 1374
Size: 2912 1857
SfcI C'TryA_G
Cuts at: 33 1812 2497 3465 3656 33
Size: 1779 685 968 191 1146
Fragments arranged by size:
1779 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2201 2449 2201
Size: 248 4521
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
SmaI CCC'GGG
Cuts at: 1791 1791
Size: 4769
SmlI C'TyrA_G
Cuts at: 1200 1479 1924 2839 3553 3815 1200
Size: 279 445 915 714 262 2154
Fragments arranged by size:
2154 915 714 445 279 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4769
SphI G_CATG'C
Cuts at: 1822 2322 1822
Size: 500 4269
SspI AAT'ATT
Cuts at: 1314 1314
Size: 4769
StuI AGG'CCT
Cuts at: 302 302
Size: 4769
StyI C'CwwG_G
Cuts at: 107 499 1128 1903 2257 107
Size: 392 629 775 354 2619
Fragments arranged by size:
2619 775 629 392 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2352 2485 2944 3153 2352
Size: 133 459 209 3968
Fragments arranged by size:
3968 459 209 133
TatI w'GTAC_w
Cuts at: 519 1233 1372 4284 519
Size: 714 139 2912 1004
Fragments arranged by size:
2912 1004 714 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 353 385 4286 4318 353
Size: 32 3901 32 804
Fragments arranged by size:
3901 804 32 32
Tth111I GACn'n_nGTC
Cuts at: 167 2566 167
Size: 2399 2370
XbaI T'CTAG_A
Cuts at: 1578 1800 1578
Size: 222 4547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1078 1410 2264 1078
Size: 332 854 3583
Fragments arranged by size:
3583 854 332
XmaI C'CCGG_G
Cuts at: 1789 1789
Size: 4769
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PmeI PpiI PsiI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BsiWI BsmI BsmBI BspEI BsrGI
BssHII BstXI Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI