Restriction map for
pF1KA0878
(Circular) (Six-base) MAPSORT of: pF1KA0878.seq Check: 5375 from: 1 to: 4946
pF1KA0878 4946 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1984 1984
Size: 4946
Acc65I G'GTAC_C
Cuts at: 508 1962 508
Size: 1454 3492
AclI AA'CG_TT
Cuts at: 410 4786 410
Size: 4376 570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 833 1055 1779 2771 3203 3950 4218 605
Size: 228 222 724 992 432 747 268 1333
Fragments arranged by size:
1333 992 747 724 432 268 228 222
AflIII A'CryG_T
Cuts at: 2298 3402 4379 2298
Size: 1104 977 2865
Fragments arranged by size:
2865 1104 977
AgeI A'CCGG_T
Cuts at: 3334 3334
Size: 4946
AleI CACnn'nnGTG
Cuts at: 1634 1634
Size: 4946
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 250 250
Size: 4946
AlwNI CAG_nnn'CTG
Cuts at: 2251 3818 2251
Size: 1567 3379
ApaLI G'TGCA_C
Cuts at: 3716 3716
Size: 4946
ApoI r'AATT_y
Cuts at: 477 1382 1810 1950 4299 4555 477
Size: 905 428 140 2349 256 868
Fragments arranged by size:
2349 905 868 428 256 140
AseI AT'TA_AT
Cuts at: 20 1271 20
Size: 1251 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
AvaI C'yCGr_G
Cuts at: 1966 1966
Size: 4946
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1382 1415 1382
Size: 33 4913
BamHI G'GATC_C
Cuts at: 590 1971 590
Size: 1381 3565
BanI G'GyrC_C
Cuts at: 508 1962 2376 2624 2659 508
Size: 1454 414 248 35 2795
Fragments arranged by size:
2795 1454 414 248 35
BanII G_rGCy'C
Cuts at: 1960 2990 1960
Size: 1030 3916
BbeI G_GCGC'C
Cuts at: 2380 2628 2380
Size: 248 4698
BbsI GAAGACnn'nnnn_
Cuts at: 718 1368 718
Size: 650 4296
BciVI GTATCCnnnnn_n'
Cuts at: 2844 3605 4946 2844
Size: 761 1341 2844
Fragments arranged by size:
2844 1341 761
BclI T'GATC_A
Cuts at: 398 2466 398
Size: 2068 2878
BfrBI ATG'CAT
Cuts at: 1247 1247
Size: 4946
BglI GCCn_nnn'nGGC
Cuts at: 174 1411 1565 174
Size: 1237 154 3555
Fragments arranged by size:
3555 1237 154
BglII A'GATC_T
Cuts at: 2461 4498 2461
Size: 2037 2909
BlpI GC'TnA_GC
Cuts at: 2058 2058
Size: 4946
Bme1580I G_kGCm'C
Cuts at: 2571 2664 3720 2571
Size: 93 1056 3797
Fragments arranged by size:
3797 1056 93
BmgBI CAC'GTC
Cuts at: 873 873
Size: 4946
BmrI ACTGGGnnnn_n'
Cuts at: 2158 2239 2574 4718 2158
Size: 81 335 2144 2386
Fragments arranged by size:
2386 2144 335 81
BmtI G_CTAG'C
Cuts at: 1061 1061
Size: 4946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 221 3313 221
Size: 3092 1854
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 2122 3001 3493 3791 1321
Size: 801 879 492 298 2476
Fragments arranged by size:
2476 879 801 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 216 4650 216
Size: 4434 512
BsaAI yAC'GTr
Cuts at: 2299 2299
Size: 4946
BsaBI GATnn'nnATC
Cuts at: 6 2460 4497 6
Size: 2454 2037 455
Fragments arranged by size:
2454 2037 455
BsaHI Gr'CG_yC
Cuts at: 1106 2377 2625 3327 4823 1106
Size: 1271 248 702 1496 1229
Fragments arranged by size:
1496 1271 1229 702 248
BsaWI w'CCGG_w
Cuts at: 264 2135 2656 3334 3608 3755 4166 264
Size: 1871 521 678 274 147 411 1044
Fragments arranged by size:
1871 1044 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1534 1564 1534
Size: 30 4916
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 686 686
Size: 4946
BseYI C'CCAG_C
Cuts at: 129 1560 1655 2371 3129 3706 4202 129
Size: 1431 95 716 758 577 496 873
Fragments arranged by size:
1431 873 758 716 577 496 95
BsiEI CG_ry'CG
Cuts at: 104 169 257 2488 2534 3742 104
Size: 65 88 2231 46 1208 1308
Fragments arranged by size:
2231 1308 1208 88 65 46
BsiHKAI G_wGCw'C
Cuts at: 1960 2738 2928 3720 1960
Size: 778 190 792 3186
Fragments arranged by size:
3186 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1960 2571 2664 2738 2928 2990 3720 1960
Size: 611 93 74 190 62 730 3186
Fragments arranged by size:
3186 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4166 4166
Size: 4946
BspHI T'CATG_A
Cuts at: 4122 4122
Size: 4946
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1721 1996 2210 2512 2893 1721
Size: 275 214 302 381 3774
Fragments arranged by size:
3774 381 302 275 214
BsrBI CCG'CTC
Cuts at: 50 3238 3292 4776 4941 50
Size: 3188 54 1484 165 55
Fragments arranged by size:
3188 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1772 2858 1772
Size: 1086 3860
BsrFI r'CCGG_y
Cuts at: 164 2944 3125 3334 164
Size: 2780 181 209 1776
Fragments arranged by size:
2780 1776 209 181
BsrGI T'GTAC_A
Cuts at: 535 1541 535
Size: 1006 3940
BssSI C'ACGA_G
Cuts at: 3217 3575 3217
Size: 358 4588
BstAPI GCAn_nnn'nTGC
Cuts at: 1668 2323 1668
Size: 655 4291
BstBI TT'CG_AA
Cuts at: 1379 3307 1379
Size: 1928 3018
BstXI CCAn_nnnn'nTGG
Cuts at: 1422 1422
Size: 4946
BstYI r'GATC_y
Cuts at: 1 590 1017 1818 1971 2438 2461 2796 3042
Size: 589 427 801 153 467 23 335 246
Cuts at: 3042 4054 4140 4152 4498 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 801 589 467 449 427 346 335
246 153 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1279 1279
Size: 4946
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2896 3077 3240 2896
Size: 181 163 4602
Fragments arranged by size:
4602 181 163
BtsI GCAGTG_nn'
Cuts at: 2189 2189
Size: 4946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1726 1761 1726
Size: 35 4911
DraI TTT'AAA
Cuts at: 896 1253 1945 896
Size: 357 692 3897
Fragments arranged by size:
3897 692 357
DraIII CAC_nnn'GTG
Cuts at: 123 123
Size: 4946
DrdI GACnn_nn'nnGTC
Cuts at: 328 1521 2652 3510 328
Size: 1193 1131 858 1764
Fragments arranged by size:
1764 1193 1131 858
EaeI y'GGCC_r
Cuts at: 166 245 1861 2531 2705 3096 3123 4468 166
Size: 79 1616 670 174 391 27 1345 644
Fragments arranged by size:
1616 1345 670 644 391 174 79 27
EagI C'GGCC_G
Cuts at: 166 2531 166
Size: 2365 2581
EarI CTCTTCn'nnn_
Cuts at: 518 964 1117 2969 3179 518
Size: 446 153 1852 210 2285
Fragments arranged by size:
2285 1852 446 210 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3154 3464 3610 4756 3154
Size: 310 146 1146 3344
Fragments arranged by size:
3344 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1958 1958
Size: 4946
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 833 1055 1779 2771 3203 3950 4218 605
Size: 228 222 724 992 432 747 268 1333
Fragments arranged by size:
1333 992 747 724 432 268 228 222
EcoO109I rG'GnC_Cy
Cuts at: 2085 2085
Size: 4946
EcoRI G'AATT_C
Cuts at: 1950 1950
Size: 4946
EcoRV GAT'ATC
Cuts at: 1188 1188
Size: 4946
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2965 2965
Size: 4946
FspI TGC'GCA
Cuts at: 2727 2727
Size: 4946
HaeII r_GCGC'y
Cuts at: 130 386 2380 2628 3650 4629 130
Size: 256 1994 248 1022 979 447
Fragments arranged by size:
1994 1022 979 447 256 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2788 2818 2820 2850 2788
Size: 30 2 30 4884
Fragments arranged by size:
4884 30 30 2
HincII GTy'rAC
Cuts at: 1710 1985 1710
Size: 275 4671
KasI G'GCGC_C
Cuts at: 2376 2624 2376
Size: 248 4698
KpnI G_GTAC'C
Cuts at: 512 1966 512
Size: 1454 3492
MluI A'CGCG_T
Cuts at: 4379 4379
Size: 4946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 618 659 3617 3801 618
Size: 41 2958 184 1763
Fragments arranged by size:
2958 1763 184 41
MscI TGG'CCA
Cuts at: 2707 2707
Size: 4946
MslI CAynn'nnrTG
Cuts at: 394 1420 1634 2935 3062 394
Size: 1026 214 1301 127 2278
Fragments arranged by size:
2278 1301 1026 214 127
MspA1I CmG'CkG
Cuts at: 1564 1659 2057 2371 2731 3744 3989 1564
Size: 95 398 314 360 1013 245 2521
Fragments arranged by size:
2521 1013 398 360 314 245 95
NaeI GCC'GGC
Cuts at: 3127 3127
Size: 4946
NarI GG'CG_CC
Cuts at: 2377 2625 2377
Size: 248 4698
NgoMIV G'CCGG_C
Cuts at: 3125 3125
Size: 4946
NheI G'CTAG_C
Cuts at: 1057 1057
Size: 4946
NsiI A_TGCA'T
Cuts at: 1249 1249
Size: 4946
NspI r_CATG'y
Cuts at: 542 1671 1999 2499 3406 542
Size: 1129 328 500 907 2082
Fragments arranged by size:
2082 1129 907 500 328
PciI A'CATG_T
Cuts at: 3402 3402
Size: 4946
PflMI CCAn_nnn'nTGG
Cuts at: 123 867 123
Size: 744 4202
PfoI T'CCnGG_A
Cuts at: 1915 1915
Size: 4946
PmeI GTTT'AAAC
Cuts at: 1945 1945
Size: 4946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4085 4117 4085
Size: 32 4914
PsiI TTA'TAA
Cuts at: 827 884 1325 827
Size: 57 441 4448
Fragments arranged by size:
4448 441 57
PstI C_TGCA'G
Cuts at: 1628 1664 1993 2678 1628
Size: 36 329 685 3896
Fragments arranged by size:
3896 685 329 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4946
PvuII CAG'CTG
Cuts at: 1564 1659 2371 2731 1564
Size: 95 712 360 3779
Fragments arranged by size:
3779 712 360 95
RsrII CG'GwC_CG
Cuts at: 3141 3141
Size: 4946
SacI G_AGCT'C
Cuts at: 1960 1960
Size: 4946
SalI G'TCGA_C
Cuts at: 1983 1983
Size: 4946
SapI GCTCTTCn'nnn_
Cuts at: 2969 3179 2969
Size: 210 4736
SbfI CC_TGCA'GG
Cuts at: 1993 1993
Size: 4946
ScaI AGT'ACT
Cuts at: 4463 4463
Size: 4946
SfcI C'TryA_G
Cuts at: 33 1624 1660 1989 2674 3667 3858 33
Size: 1591 36 329 685 993 191 1121
Fragments arranged by size:
1591 1121 993 685 329 191 36
SfoI GGC'GCC
Cuts at: 2378 2626 2378
Size: 248 4698
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4946
SmaI CCC'GGG
Cuts at: 1968 1968
Size: 4946
SmlI C'TyrA_G
Cuts at: 1336 2101 3016 3508 3770 1336
Size: 765 915 492 262 2512
Fragments arranged by size:
2512 915 765 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4946
SphI G_CATG'C
Cuts at: 1671 1999 2499 1671
Size: 328 500 4118
Fragments arranged by size:
4118 500 328
StyI C'CwwG_G
Cuts at: 2080 2434 2080
Size: 354 4592
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2529 2662 3121 3330 2529
Size: 133 459 209 4145
Fragments arranged by size:
4145 459 209 133
TatI w'GTAC_w
Cuts at: 535 577 1541 1874 4461 535
Size: 42 964 333 2587 1020
Fragments arranged by size:
2587 1020 964 333 42
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4463 4495 4463
Size: 32 4914
Tth111I GACn'n_nGTC
Cuts at: 2743 2743
Size: 4946
XbaI T'CTAG_A
Cuts at: 1977 1977
Size: 4946
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 120 2441 120
Size: 2321 2625
XmaI C'CCGG_G
Cuts at: 1966 1966
Size: 4946
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NsiI
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BbvCI BcgI BpmI Bpu10I BsgI BsiWI BsmI
BsmBI BssHII BstEII BstZ17I BtgI ClaI EcoNI FseI
FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI