Restriction map for
pF1KA0879
(Circular) (Six-base) MAPSORT of: pF1KA0879.seq Check: 4783 from: 1 to: 4472
pF1KA0879 4472 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 1510 1510
Size: 4472
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 250 466 719 1414 2297 2729 3476 3744
Size: 52 216 253 695 883 432 747 268
Cuts at: 3744 198
Size: 926
Fragments arranged by size:
926 883 747 695 432 268 253 216
52
AflIII A'CryG_T
Cuts at: 1824 2928 3905 1824
Size: 1104 977 2391
Fragments arranged by size:
2391 1104 977
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AhdI GACnn_n'nnGTC
Cuts at: 1274 1274
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 913 1777 3344 913
Size: 864 1567 2041
Fragments arranged by size:
2041 1567 864
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 241 258 1037 1476 3825 4081 241
Size: 17 779 439 2349 256 632
Fragments arranged by size:
2349 779 632 439 256 17
AseI AT'TA_AT
Cuts at: 20 1313 20
Size: 1293 3179
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 1492 1492
Size: 4472
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 512 545 512
Size: 33 4439
BamHI G'GATC_C
Cuts at: 432 1497 432
Size: 1065 3407
BanI G'GyrC_C
Cuts at: 1488 1902 2150 2185 1488
Size: 414 248 35 3775
Fragments arranged by size:
3775 414 248 35
BanII G_rGCy'C
Cuts at: 907 1486 2516 907
Size: 579 1030 2863
Fragments arranged by size:
2863 1030 579
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 1004 1004
Size: 4472
BciVI GTATCCnnnnn_n'
Cuts at: 210 2370 3131 4472 210
Size: 2160 761 1341 210
Fragments arranged by size:
2160 1341 761 210
BclI T'GATC_A
Cuts at: 803 816 1992 803
Size: 13 1176 3283
Fragments arranged by size:
3283 1176 13
BfrBI ATG'CAT
Cuts at: 1143 1143
Size: 4472
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 825 901 1684 1765 2100 4244 825
Size: 76 783 81 335 2144 1053
Fragments arranged by size:
2144 1053 783 335 81 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 238
Size: 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1648 2527 3019 3317 754
Size: 894 879 492 298 1909
Fragments arranged by size:
1909 894 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 1217 1339 1451 1986 4023 6
Size: 1211 122 112 535 2037 455
Fragments arranged by size:
2037 1211 535 455 122 112
BsaHI Gr'CG_yC
Cuts at: 1903 2151 2853 4349 1903
Size: 248 702 1496 2026
Fragments arranged by size:
2026 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 564 564
Size: 4472
BseYI C'CCAG_C
Cuts at: 1897 2655 3232 3728 1897
Size: 758 577 496 2641
Fragments arranged by size:
2641 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1486 2264 2454 3246 1486
Size: 778 190 792 2712
Fragments arranged by size:
2712 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 907 1486 2097 2190 2264 2454 2516 3246 907
Size: 579 611 93 74 190 62 730 2133
Fragments arranged by size:
2133 730 611 579 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1176 1522 1736 2038 2419 1176
Size: 346 214 302 381 3229
Fragments arranged by size:
3229 381 346 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4472
BstBI TT'CG_AA
Cuts at: 628 2833 628
Size: 2205 2267
BstEII G'GTnAC_C
Cuts at: 468 1108 468
Size: 640 3832
BstXI CCAn_nnnn'nTGG
Cuts at: 1053 1053
Size: 4472
BstYI r'GATC_y
Cuts at: 1 432 1497 1964 1987 2322 2568 3580 3666
Size: 431 1065 467 23 335 246 1012 86
Cuts at: 3666 3678 4024 1
Size: 12 346 449
Fragments arranged by size:
1065 1012 467 449 431 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 363 1113 1161 363
Size: 750 48 3674
Fragments arranged by size:
3674 750 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1314 2422 2603 2766 1314
Size: 1108 181 163 3020
Fragments arranged by size:
3020 1108 181 163
BtsI GCAGTG_nn'
Cuts at: 1715 1715
Size: 4472
ClaI AT'CG_AT
Cuts at: 872 872
Size: 4472
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 681 2057 2231 2622 2649 3994 681
Size: 1376 174 391 27 1345 1159
Fragments arranged by size:
1376 1345 1159 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 171 555 2495 2705 171
Size: 384 1940 210 1938
Fragments arranged by size:
1940 1938 384 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 238 250 466 719 1414 2297 2729 3476
Size: 40 12 216 253 695 883 432 747
Cuts at: 3476 3744 198
Size: 268 926
Fragments arranged by size:
926 883 747 695 432 268 253 216
40 12
EcoO109I rG'GnC_Cy
Cuts at: 1268 1611 1268
Size: 343 4129
EcoRI G'AATT_C
Cuts at: 1037 1476 1037
Size: 439 4033
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 182 426 2491 182
Size: 244 2065 2163
Fragments arranged by size:
2163 2065 244
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 154 186 532 564 1260 1292 2314 2344 2346
Size: 32 346 32 696 32 1022 30 2
Cuts at: 2346 2376 154
Size: 30 2250
Fragments arranged by size:
2250 1022 696 346 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1299 1361 1511 1299
Size: 62 150 4260
Fragments arranged by size:
4260 150 62
HpaI GTT'AAC
Cuts at: 1361 1361
Size: 4472
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MfeI C'AATT_G
Cuts at: 1076 1076
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3143 3327 3143
Size: 184 4288
MscI TGG'CCA
Cuts at: 683 2233 683
Size: 1550 2922
MslI CAynn'nnrTG
Cuts at: 980 1118 2461 2588 980
Size: 138 1343 127 2864
Fragments arranged by size:
2864 1343 138 127
MspA1I CmG'CkG
Cuts at: 1583 1897 2257 3270 3515 1583
Size: 314 360 1013 245 2540
Fragments arranged by size:
2540 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NcoI C'CATG_G
Cuts at: 363 1113 363
Size: 750 3722
NdeI CA'TA_TG
Cuts at: 977 977
Size: 4472
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NruI TCG'CGA
Cuts at: 1333 1333
Size: 4472
NsiI A_TGCA'T
Cuts at: 1145 1145
Size: 4472
NspI r_CATG'y
Cuts at: 292 1376 1525 2025 2932 292
Size: 1084 149 500 907 1832
Fragments arranged by size:
1832 1084 907 500 149
PciI A'CATG_T
Cuts at: 2928 2928
Size: 4472
PflMI CCAn_nnn'nTGG
Cuts at: 1239 1239
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PpuMI rG'GwC_Cy
Cuts at: 1268 1268
Size: 4472
PsiI TTA'TAA
Cuts at: 145 950 145
Size: 805 3667
PstI C_TGCA'G
Cuts at: 1519 2204 1519
Size: 685 3787
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 1897 2257 1897
Size: 360 4112
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 330 966 1515 2200 3193 3384 33
Size: 297 636 549 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 549 297 191
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 769 1627 2542 3034 3296 769
Size: 858 915 492 262 1945
Fragments arranged by size:
1945 915 858 492 262
SpeI A'CTAG_T
Cuts at: 67 195 67
Size: 128 4344
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
SspI AAT'ATT
Cuts at: 606 606
Size: 4472
StyI C'CwwG_G
Cuts at: 363 1113 1606 1960 363
Size: 750 493 354 2875
Fragments arranged by size:
2875 750 493 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 456 2055 2188 2647 2856 456
Size: 1599 133 459 209 2072
Fragments arranged by size:
2072 1599 459 209 133
TatI w'GTAC_w
Cuts at: 3987 3987
Size: 4472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4440
Tth111I GACn'n_nGTC
Cuts at: 757 2269 757
Size: 1512 2960
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1967 1967
Size: 4472
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
XmnI GAAnn'nnTTC
Cuts at: 1001 1001
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
NheI NotI PacI PasI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI