Restriction map for
pF1KA0882
(Circular) (Six-base) MAPSORT of: pF1KA0882.seq Check: 1324 from: 1 to: 6911
pF1KA0882 6911 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 329 719 2411 3949 329
Size: 390 1692 1538 3291
Fragments arranged by size:
3291 1692 1538 390
Acc65I G'GTAC_C
Cuts at: 250 3927 250
Size: 3677 3234
AclI AA'CG_TT
Cuts at: 6751 6751
Size: 6911
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1913 4736 5168 5915 6183 1913
Size: 2823 432 747 268 2641
Fragments arranged by size:
2823 2641 747 432 268
AflIII A'CryG_T
Cuts at: 3003 3221 4263 5367 6344 3003
Size: 218 1042 1104 977 3570
Fragments arranged by size:
3570 1104 1042 977 218
AgeI A'CCGG_T
Cuts at: 5299 5299
Size: 6911
AleI CACnn'nnGTG
Cuts at: 448 1930 448
Size: 1482 5429
AlwNI CAG_nnn'CTG
Cuts at: 1494 2499 2517 2950 3252 3756 4216 5783 1494
Size: 1005 18 433 302 504 460 1567 2622
Fragments arranged by size:
2622 1567 1005 504 460 433 302 18
ApaLI G'TGCA_C
Cuts at: 995 2030 5681 995
Size: 1035 3651 2225
Fragments arranged by size:
3651 2225 1035
ApoI r'AATT_y
Cuts at: 547 2446 3093 3915 6264 6520 547
Size: 1899 647 822 2349 256 938
Fragments arranged by size:
2349 1899 938 822 647 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6911
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6911
AvaI C'yCGr_G
Cuts at: 281 1522 3527 3931 281
Size: 1241 2005 404 3261
Fragments arranged by size:
3261 2005 1241 404
BamHI G'GATC_C
Cuts at: 3936 3936
Size: 6911
BanI G'GyrC_C
Cuts at: 250 1696 3927 4341 4589 4624 250
Size: 1446 2231 414 248 35 2537
Fragments arranged by size:
2537 2231 1446 414 248 35
BanII G_rGCy'C
Cuts at: 1429 1679 3443 3925 4955 1429
Size: 250 1764 482 1030 3385
Fragments arranged by size:
3385 1764 1030 482 250
BbeI G_GCGC'C
Cuts at: 4345 4593 4345
Size: 248 6663
BbsI GAAGACnn'nnnn_
Cuts at: 1742 3239 1742
Size: 1497 5414
BbvCI CC'TCA_GC
Cuts at: 649 649
Size: 6911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2691 2725 3297 3331 2691
Size: 34 572 34 6271
Fragments arranged by size:
6271 572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2517 4809 5570 6911 2517
Size: 2292 761 1341 2517
Fragments arranged by size:
2517 2292 1341 761
BclI T'GATC_A
Cuts at: 767 2931 4431 767
Size: 2164 1500 3247
Fragments arranged by size:
3247 2164 1500
BglI GCCn_nnn'nGGC
Cuts at: 206 3374 3457 206
Size: 3168 83 3660
Fragments arranged by size:
3660 3168 83
BglII A'GATC_T
Cuts at: 4426 6463 4426
Size: 2037 4874
BlpI GC'TnA_GC
Cuts at: 2380 4023 2380
Size: 1643 5268
Bme1580I G_kGCm'C
Cuts at: 999 2034 3694 4536 4629 5685 999
Size: 1035 1660 842 93 1056 2225
Fragments arranged by size:
2225 1660 1056 1035 842 93
BmgBI CAC'GTC
Cuts at: 2910 2910
Size: 6911
BmrI ACTGGGnnnn_n'
Cuts at: 387 1202 2646 3741 4123 4204 4539 6683 387
Size: 815 1444 1095 382 81 335 2144 615
Fragments arranged by size:
2144 1444 1095 815 615 382 335 81
BmtI G_CTAG'C
Cuts at: 2065 2065
Size: 6911
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 2859 2943 3495 3537 5278 1395
Size: 1464 84 552 42 1741 3028
Fragments arranged by size:
3028 1741 1464 552 84 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 299 1179 1389 2539 3116 3310 3532 258
Size: 41 880 210 1150 577 194 222 3637
Fragments arranged by size:
3637 1150 880 577 222 210 194 41
Bpu10I CC'TnA_GC
Cuts at: 649 2918 649
Size: 2269 4642
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 754 4087 4966 5458 5756 409
Size: 345 3333 879 492 298 1564
Fragments arranged by size:
3333 1564 879 492 345 298
BsaI GGTCTCn'nnnn_
Cuts at: 820 1523 6615 820
Size: 703 5092 1116
Fragments arranged by size:
5092 1116 703
BsaAI yAC'GTr
Cuts at: 3423 4264 3423
Size: 841 6070
BsaBI GATnn'nnATC
Cuts at: 6 4425 6462 6
Size: 4419 2037 455
Fragments arranged by size:
4419 2037 455
BsaHI Gr'CG_yC
Cuts at: 4342 4590 5292 6788 4342
Size: 248 702 1496 4465
Fragments arranged by size:
4465 1496 702 248
BsaWI w'CCGG_w
Cuts at: 710 3845 4100 4621 5299 5573 5720 6131 710
Size: 3135 255 521 678 274 147 411 1490
Fragments arranged by size:
3135 1490 678 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 115 145 1627 1657 2337 2356 2367 2386 3571
Size: 30 1482 30 680 19 11 19 1185
Cuts at: 3571 3601 115
Size: 30 3425
Fragments arranged by size:
3425 1482 1185 680 30 30 30 19
19 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 1144 1392 1540 1795 1969 2071 3510 3537
Size: 1005 248 148 255 174 102 1439 27
Cuts at: 3537 3643 139
Size: 106 3407
Fragments arranged by size:
3407 1439 1005 255 248 174 148 106
102 27
BseYI C'CCAG_C
Cuts at: 1387 1821 2674 3323 4336 5094 5671 6167 1387
Size: 434 853 649 1013 758 577 496 2131
Fragments arranged by size:
2131 1013 853 758 649 577 496 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1303 1835 2198 3059 3625 1029
Size: 274 532 363 861 566 4315
Fragments arranged by size:
4315 861 566 532 363 274
BsiEI CG_ry'CG
Cuts at: 104 4453 4499 5707 104
Size: 4349 46 1208 1308
Fragments arranged by size:
4349 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 781 999 1205 1429 1679 2034 3392 3715 3925
Size: 218 206 224 250 355 1358 323 210
Cuts at: 3925 4703 4893 5685 781
Size: 778 190 792 2007
Fragments arranged by size:
2007 1358 792 778 355 323 250 224
218 210 206 190
BsmI GAATG_Cn'
Cuts at: 747 3162 747
Size: 2415 4496
BsmBI CGTCTCn'nnnn_
Cuts at: 1404 1404
Size: 6911
Bsp1286I G_dGCh'C
Cuts at: 781 999 1205 1429 1679 2034 3392 3443 3694
Size: 218 206 224 250 355 1358 51 251
Cuts at: 3694 3715 3925 4536 4629 4703 4893 4955 5685
Size: 21 210 611 93 74 190 62 730
Cuts at: 5685 781
Size: 2007
Fragments arranged by size:
2007 1358 730 611 355 251 250 224
218 210 206 190 93 74 62 51
21
BspEI T'CCGG_A
Cuts at: 3845 6131 3845
Size: 2286 4625
BspHI T'CATG_A
Cuts at: 6087 6087
Size: 6911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3961 4175 4477 4858 3961
Size: 214 302 381 6014
Fragments arranged by size:
6014 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1158 1805 3347 3683 5203 5257 6741 6906
Size: 1108 647 1542 336 1520 54 1484 165
Cuts at: 6906 50
Size: 55
Fragments arranged by size:
1542 1520 1484 1108 647 336 165 55
54
BsrDI GCAATG_nn'
Cuts at: 473 1844 4823 473
Size: 1371 2979 2561
Fragments arranged by size:
2979 2561 1371
BsrFI r'CCGG_y
Cuts at: 205 2663 3447 3616 4909 5090 5299 205
Size: 2458 784 169 1293 181 209 1817
Fragments arranged by size:
2458 1817 1293 784 209 181 169
BsrGI T'GTAC_A
Cuts at: 2897 2998 2897
Size: 101 6810
BssSI C'ACGA_G
Cuts at: 1422 2066 3625 5182 5540 1422
Size: 644 1559 1557 358 2793
Fragments arranged by size:
2793 1559 1557 644 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2913 4288 2913
Size: 1375 5536
BstBI TT'CG_AA
Cuts at: 1884 5272 1884
Size: 3388 3523
BstXI CCAn_nnnn'nTGG
Cuts at: 2025 2025
Size: 6911
BstYI r'GATC_y
Cuts at: 1 1289 1604 3936 4403 4426 4761 5007 6019
Size: 1288 315 2332 467 23 335 246 1012
Cuts at: 6019 6105 6117 6463 1
Size: 86 12 346 449
Fragments arranged by size:
2332 1288 1012 467 449 346 335 315
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2405 2405
Size: 6911
BtgI C'CryG_G
Cuts at: 1754 1775 2865 3263 1754
Size: 21 1090 398 5402
Fragments arranged by size:
5402 1090 398 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 224 324 2399 4861 5042 5205 224
Size: 100 2075 2462 181 163 1930
Fragments arranged by size:
2462 2075 1930 181 163 100
BtsI GCAGTG_nn'
Cuts at: 2347 3278 3360 3642 3700 4154 2347
Size: 931 82 282 58 454 5104
Fragments arranged by size:
5104 931 454 282 82 58
ClaI AT'CG_AT
Cuts at: 903 903
Size: 6911
DraI TTT'AAA
Cuts at: 531 3910 531
Size: 3379 3532
DrdI GACnn_nn'nnGTC
Cuts at: 325 2047 4617 5475 325
Size: 1722 2570 858 1761
Fragments arranged by size:
2570 1761 1722 858
EaeI y'GGCC_r
Cuts at: 137 1032 1544 3449 3684 3818 3872 3893 4496
Size: 895 512 1905 235 134 54 21 603
Cuts at: 4496 4670 5061 5088 6433 137
Size: 174 391 27 1345 615
Fragments arranged by size:
1905 1345 895 615 603 512 391 235
174 134 54 27 21
EagI C'GGCC_G
Cuts at: 4496 4496
Size: 6911
EarI CTCTTCn'nnn_
Cuts at: 572 2741 3079 3397 4934 5144 572
Size: 2169 338 318 1537 210 2339
Fragments arranged by size:
2339 2169 1537 338 318 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 242 5119 5429 5575 6721 242
Size: 4877 310 146 1146 432
Fragments arranged by size:
4877 1146 432 310 146
EcoICRI GAG'CTC
Cuts at: 1427 1677 3923 1427
Size: 250 2246 4415
Fragments arranged by size:
4415 2246 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 258 299 1179 1389 1913 2539 3116 3310 3532
Size: 41 880 210 524 626 577 194 222
Cuts at: 3532 4736 5168 5915 6183 258
Size: 1204 432 747 268 986
Fragments arranged by size:
1204 986 880 747 626 577 524 432
268 222 210 194 41
EcoO109I rG'GnC_Cy
Cuts at: 624 2871 3539 4050 624
Size: 2247 668 511 3485
Fragments arranged by size:
3485 2247 668 511
EcoRI G'AATT_C
Cuts at: 3915 3915
Size: 6911
EcoRV GAT'ATC
Cuts at: 438 438
Size: 6911
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1599 4930 1599
Size: 3331 3580
FspI TGC'GCA
Cuts at: 4692 4692
Size: 6911
HaeII r_GCGC'y
Cuts at: 281 1449 4345 4593 5615 6594 281
Size: 1168 2896 248 1022 979 598
Fragments arranged by size:
2896 1168 1022 979 598 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1491 1523 2060 2092 3571 3603 4753 4783 4785
Size: 32 537 32 1479 32 1150 30 2
Cuts at: 4785 4815 1491
Size: 30 3587
Fragments arranged by size:
3587 1479 1150 537 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 591 2193 3950 591
Size: 1602 1757 3552
Fragments arranged by size:
3552 1757 1602
HindIII A'AGCT_T
Cuts at: 1961 1961
Size: 6911
KasI G'GCGC_C
Cuts at: 4341 4589 4341
Size: 248 6663
KpnI G_GTAC'C
Cuts at: 254 3931 254
Size: 3677 3234
MfeI C'AATT_G
Cuts at: 1172 1172
Size: 6911
MluI A'CGCG_T
Cuts at: 6344 6344
Size: 6911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 248 619 2991 5582 5766 248
Size: 371 2372 2591 184 1393
Fragments arranged by size:
2591 2372 1393 371 184
MscI TGG'CCA
Cuts at: 139 1034 1546 3820 4672 139
Size: 895 512 2274 852 2378
Fragments arranged by size:
2378 2274 895 852 512
MslI CAynn'nnrTG
Cuts at: 112 448 862 1930 3847 4900 5027 112
Size: 336 414 1068 1917 1053 127 1996
Fragments arranged by size:
1996 1917 1068 1053 414 336 127
MspA1I CmG'CkG
Cuts at: 189 2085 2630 4022 4336 4696 5709 5954 189
Size: 1896 545 1392 314 360 1013 245 1146
Fragments arranged by size:
1896 1392 1146 1013 545 360 314 245
NaeI GCC'GGC
Cuts at: 207 3449 3618 5092 207
Size: 3242 169 1474 2026
Fragments arranged by size:
3242 2026 1474 169
NarI GG'CG_CC
Cuts at: 4342 4590 4342
Size: 248 6663
NcoI C'CATG_G
Cuts at: 1754 2865 1754
Size: 1111 5800
NgoMIV G'CCGG_C
Cuts at: 205 3447 3616 5090 205
Size: 3242 169 1474 2026
Fragments arranged by size:
3242 2026 1474 169
NheI G'CTAG_C
Cuts at: 2061 2061
Size: 6911
NspI r_CATG'y
Cuts at: 1614 1668 1998 2100 2756 2864 3007 3225 3964
Size: 54 330 102 656 108 143 218 739
Cuts at: 3964 4464 5371 1614
Size: 500 907 3154
Fragments arranged by size:
3154 907 739 656 500 330 218 143
108 102 54
PciI A'CATG_T
Cuts at: 3003 3221 5367 3003
Size: 218 2146 4547
Fragments arranged by size:
4547 2146 218
PflMI CCAn_nnn'nTGG
Cuts at: 2517 2517
Size: 6911
PfoI T'CCnGG_A
Cuts at: 355 1655 2831 355
Size: 1300 1176 4435
Fragments arranged by size:
4435 1300 1176
PmeI GTTT'AAAC
Cuts at: 3910 3910
Size: 6911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6050 6082 6050
Size: 32 6879
PpuMI rG'GwC_Cy
Cuts at: 624 2871 624
Size: 2247 4664
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3208 3240 3208
Size: 32 6879
PstI C_TGCA'G
Cuts at: 188 3958 4643 188
Size: 3770 685 2456
Fragments arranged by size:
3770 2456 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6911
PvuII CAG'CTG
Cuts at: 2085 2630 4336 4696 2085
Size: 545 1706 360 4300
Fragments arranged by size:
4300 1706 545 360
RsrII CG'GwC_CG
Cuts at: 5106 5106
Size: 6911
SacI G_AGCT'C
Cuts at: 1429 1679 3925 1429
Size: 250 2246 4415
Fragments arranged by size:
4415 2246 250
SalI G'TCGA_C
Cuts at: 3948 3948
Size: 6911
SapI GCTCTTCn'nnn_
Cuts at: 2741 3397 4934 5144 2741
Size: 656 1537 210 4508
Fragments arranged by size:
4508 1537 656 210
SbfI CC_TGCA'GG
Cuts at: 3958 3958
Size: 6911
ScaI AGT'ACT
Cuts at: 2971 3793 6428 2971
Size: 822 2635 3454
Fragments arranged by size:
3454 2635 822
SfcI C'TryA_G
Cuts at: 33 184 3954 4639 5632 5823 33
Size: 151 3770 685 993 191 1121
Fragments arranged by size:
3770 1121 993 685 191 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 3457 3457
Size: 6911
SfoI GGC'GCC
Cuts at: 4343 4591 4343
Size: 248 6663
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6911
SgrAI Cr'CCGG_yG
Cuts at: 205 205
Size: 6911
SmaI CCC'GGG
Cuts at: 283 3529 3933 283
Size: 3246 404 3261
Fragments arranged by size:
3261 3246 404
SmlI C'TyrA_G
Cuts at: 388 733 4066 4981 5473 5735 388
Size: 345 3333 915 492 262 1564
Fragments arranged by size:
3333 1564 915 492 345 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6911
SphI G_CATG'C
Cuts at: 1668 1998 2100 2864 3964 4464 1668
Size: 330 102 764 1100 500 4115
Fragments arranged by size:
4115 1100 764 500 330 102
SspI AAT'ATT
Cuts at: 1920 1920
Size: 6911
StyI C'CwwG_G
Cuts at: 1754 2043 2746 2865 3483 3575 4045 4399 1754
Size: 289 703 119 618 92 470 354 4266
Fragments arranged by size:
4266 703 618 470 354 289 119 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 102 1835 2017 4494 4627 5086 5295 102
Size: 1733 182 2477 133 459 209 1718
Fragments arranged by size:
2477 1733 1718 459 209 182 133
TatI w'GTAC_w
Cuts at: 1376 2897 2969 2998 3791 6426 1376
Size: 1521 72 29 793 2635 1861
Fragments arranged by size:
2635 1861 1521 793 72 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2204 2236 2337 2356 2369 2388 3816 3848 6428
Size: 32 101 19 13 19 1428 32 2580
Cuts at: 6428 6460 2204
Size: 32 2655
Fragments arranged by size:
2655 2580 1428 101 32 32 32 19
19 13
Tth111I GACn'n_nGTC
Cuts at: 2074 3667 4708 2074
Size: 1593 1041 4277
Fragments arranged by size:
4277 1593 1041
XbaI T'CTAG_A
Cuts at: 3942 3942
Size: 6911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 208 2304 2583 3285 4406 208
Size: 2096 279 702 1121 2713
Fragments arranged by size:
2713 2096 1121 702 279
XmaI C'CCGG_G
Cuts at: 281 3527 3931 281
Size: 3246 404 3261
Fragments arranged by size:
3261 3246 404
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PfoI PmeI PpiI PpuMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BsiWI BssHII BstEII BstZ17I
CspCI DraIII EcoNI FseI FspAI HpaI NdeI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PspXI SacII SanDI SexAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI