Restriction map for
pF1KA0890
(Circular) (Six-base) MAPSORT of: pF1KA0890.seq Check: 9646 from: 1 to: 6695
pF1KA0890 6695 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 332 940 3286 3733 332
Size: 608 2346 447 3294
Fragments arranged by size:
3294 2346 608 447
Acc65I G'GTAC_C
Cuts at: 3711 3711
Size: 6695
AclI AA'CG_TT
Cuts at: 6535 6535
Size: 6695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 2404 2823 3541 4520 4952 5699 5967 1063
Size: 1341 419 718 979 432 747 268 1791
Fragments arranged by size:
1791 1341 979 747 718 432 419 268
AfeI AGC'GCT
Cuts at: 1528 1900 1528
Size: 372 6323
AflIII A'CryG_T
Cuts at: 4047 5151 6128 4047
Size: 1104 977 4614
Fragments arranged by size:
4614 1104 977
AgeI A'CCGG_T
Cuts at: 5083 5083
Size: 6695
AhdI GACnn_n'nnGTC
Cuts at: 729 729
Size: 6695
AleI CACnn'nnGTG
Cuts at: 192 3434 192
Size: 3242 3453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2111 3289 3638 2111
Size: 1178 349 5168
Fragments arranged by size:
5168 1178 349
AlwNI CAG_nnn'CTG
Cuts at: 1006 1356 2013 2727 4000 5567 1006
Size: 350 657 714 1273 1567 2134
Fragments arranged by size:
2134 1567 1273 714 657 350
ApaI G_GGCC'C
Cuts at: 144 1633 1701 3540 144
Size: 1489 68 1839 3299
Fragments arranged by size:
3299 1839 1489 68
ApaLI G'TGCA_C
Cuts at: 337 2218 3388 3427 5465 337
Size: 1881 1170 39 2038 1567
Fragments arranged by size:
2038 1881 1567 1170 39
ApoI r'AATT_y
Cuts at: 3699 6048 6304 3699
Size: 2349 256 4090
Fragments arranged by size:
4090 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
AvaI C'yCGr_G
Cuts at: 227 1159 1688 2828 2978 3715 227
Size: 932 529 1140 150 737 3207
Fragments arranged by size:
3207 1140 932 737 529 150
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1902 1935 3489 3522 1902
Size: 33 1554 33 5075
Fragments arranged by size:
5075 1554 33 33
BamHI G'GATC_C
Cuts at: 2117 3720 2117
Size: 1603 5092
BanI G'GyrC_C
Cuts at: 922 989 1155 1549 1938 2021 2027 3446 3711
Size: 67 166 394 389 83 6 1419 265
Cuts at: 3711 4125 4373 4408 922
Size: 414 248 35 3209
Fragments arranged by size:
3209 1419 414 394 389 265 248 166
83 67 35 6
BanII G_rGCy'C
Cuts at: 144 300 822 1166 1633 1701 3540 3709 4739
Size: 156 522 344 467 68 1839 169 1030
Cuts at: 4739 144
Size: 2100
Fragments arranged by size:
2100 1839 1030 522 467 344 169 156
68
BbeI G_GCGC'C
Cuts at: 993 3450 4129 4377 993
Size: 2457 679 248 3311
Fragments arranged by size:
3311 2457 679 248
BbsI GAAGACnn'nnnn_
Cuts at: 1501 2392 2602 3357 1501
Size: 891 210 755 4839
Fragments arranged by size:
4839 891 755 210
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 408 442 3348 3382 3562 3596 408
Size: 34 2906 34 180 34 3507
Fragments arranged by size:
3507 2906 180 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1732 4593 5354 6695 1732
Size: 2861 761 1341 1732
Fragments arranged by size:
2861 1732 1341 761
BclI T'GATC_A
Cuts at: 4215 4215
Size: 6695
BglI GCCn_nnn'nGGC
Cuts at: 817 1389 1760 2139 2456 2471 817
Size: 572 371 379 317 15 5041
Fragments arranged by size:
5041 572 379 371 317 15
BglII A'GATC_T
Cuts at: 4210 6247 4210
Size: 2037 4658
BlpI GC'TnA_GC
Cuts at: 434 3807 434
Size: 3373 3322
Bme1580I G_kGCm'C
Cuts at: 144 341 925 1160 1319 1554 1633 1701 2222
Size: 197 584 235 159 235 79 68 521
Cuts at: 2222 3392 3431 3540 4320 4413 5469 144
Size: 1170 39 109 780 93 1056 1370
Fragments arranged by size:
1370 1170 1056 780 584 521 235 235
197 159 109 93 79 68 39
BmgBI CAC'GTC
Cuts at: 1773 1803 3426 1773
Size: 30 1623 5042
Fragments arranged by size:
5042 1623 30
BmrI ACTGGGnnnn_n'
Cuts at: 822 1636 1951 3111 3907 3988 4323 6467 822
Size: 814 315 1160 796 81 335 2144 1050
Fragments arranged by size:
2144 1160 1050 814 796 335 315 81
BmtI G_CTAG'C
Cuts at: 535 3400 535
Size: 2865 3830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1920 3516 5062 1920
Size: 1596 1546 3553
Fragments arranged by size:
3553 1596 1546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 1777 2155 2252 2425 2527 2656 3013 3511
Size: 234 378 97 173 102 129 357 498
Cuts at: 3511 1543
Size: 4727
Fragments arranged by size:
4727 498 378 357 234 173 129 102
97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 756 1816 2575 3871 4750 5242 5540 756
Size: 1060 759 1296 879 492 298 1911
Fragments arranged by size:
1911 1296 1060 879 759 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1120 1177 6399 1120
Size: 57 5222 1416
Fragments arranged by size:
5222 1416 57
BsaAI yAC'GTr
Cuts at: 336 4048 336
Size: 3712 2983
BsaBI GATnn'nnATC
Cuts at: 6 4209 6246 6
Size: 4203 2037 455
Fragments arranged by size:
4203 2037 455
BsaHI Gr'CG_yC
Cuts at: 570 990 3447 4126 4374 5076 6572 570
Size: 420 2457 679 248 702 1496 693
Fragments arranged by size:
2457 1496 702 693 679 420 248
BsaWI w'CCGG_w
Cuts at: 573 3884 4405 5083 5357 5504 5915 573
Size: 3311 521 678 274 147 411 1353
Fragments arranged by size:
3311 1353 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2398 2428 2599 2629 2959 2989 2398
Size: 30 171 30 330 30 6104
Fragments arranged by size:
6104 330 171 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 691 700 1348 2962 3151 3154 3157 3369 3619
Size: 9 648 1614 189 3 3 212 250
Cuts at: 3619 691
Size: 3767
Fragments arranged by size:
3767 1614 648 250 212 189 9 3
3
BseYI C'CCAG_C
Cuts at: 143 740 1119 1130 1403 1511 1750 2942 3016
Size: 597 379 11 273 108 239 1192 74
Cuts at: 3016 3592 4120 4878 5455 5951 143
Size: 576 528 758 577 496 887
Fragments arranged by size:
1192 887 758 597 577 576 528 496
379 273 239 108 74 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 1695 1999 2071 2179 2344 2779 3084 3262
Size: 1337 304 72 108 165 435 305 178
Cuts at: 3262 3566 358
Size: 304 3487
Fragments arranged by size:
3487 1337 435 305 304 304 178 165
108 72
BsiEI CG_ry'CG
Cuts at: 104 4237 4283 5491 104
Size: 4133 46 1208 1308
Fragments arranged by size:
4133 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 341 1874 1970 2222 3392 3431 3611 3709 4487
Size: 1533 96 252 1170 39 180 98 778
Cuts at: 4487 4677 5469 341
Size: 190 792 1567
Fragments arranged by size:
1567 1533 1170 792 778 252 190 180
98 96 39
BsmI GAATG_Cn'
Cuts at: 2053 2053
Size: 6695
BsmBI CGTCTCn'nnnn_
Cuts at: 1348 1348
Size: 6695
Bsp1286I G_dGCh'C
Cuts at: 144 300 341 822 925 1160 1166 1319 1554
Size: 156 41 481 103 235 6 153 235
Cuts at: 1554 1633 1701 1874 1970 2222 3392 3431 3540
Size: 79 68 173 96 252 1170 39 109
Cuts at: 3540 3611 3709 4320 4413 4487 4677 4739 5469
Size: 71 98 611 93 74 190 62 730
Cuts at: 5469 144
Size: 1370
Fragments arranged by size:
1370 1170 730 611 481 252 235 235
190 173 156 153 109 103 98 96
93 79 74 71 68 62 41 39
6
BspEI T'CCGG_A
Cuts at: 573 5915 573
Size: 5342 1353
BspHI T'CATG_A
Cuts at: 1874 5871 1874
Size: 3997 2698
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2021 3011 3488 3745 3959 4261 4642 2021
Size: 990 477 257 214 302 381 4074
Fragments arranged by size:
4074 990 477 381 302 257 214
BsrBI CCG'CTC
Cuts at: 50 438 656 1797 3558 4987 5041 6525 6690
Size: 388 218 1141 1761 1429 54 1484 165
Cuts at: 6690 50
Size: 55
Fragments arranged by size:
1761 1484 1429 1141 388 218 165 55
54
BsrDI GCAATG_nn'
Cuts at: 1566 2303 4607 1566
Size: 737 2304 3654
Fragments arranged by size:
3654 2304 737
BsrFI r'CCGG_y
Cuts at: 1383 2018 2024 2501 2938 3169 4693 4874 5083
Size: 635 6 477 437 231 1524 181 209
Cuts at: 5083 1383
Size: 2995
Fragments arranged by size:
2995 1524 635 477 437 231 209 181
6
BssSI C'ACGA_G
Cuts at: 2191 4966 5324 2191
Size: 2775 358 3562
Fragments arranged by size:
3562 2775 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4072 4072
Size: 6695
BstBI TT'CG_AA
Cuts at: 5056 5056
Size: 6695
BstEII G'GTnAC_C
Cuts at: 3210 3210
Size: 6695
BstXI CCAn_nnnn'nTGG
Cuts at: 396 2625 2772 396
Size: 2229 147 4319
Fragments arranged by size:
4319 2229 147
BstYI r'GATC_y
Cuts at: 1 2117 2537 3720 4187 4210 4545 4791 5803
Size: 2116 420 1183 467 23 335 246 1012
Cuts at: 5803 5889 5901 6247 1
Size: 86 12 346 449
Fragments arranged by size:
2116 1183 1012 467 449 420 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 3033 3033
Size: 6695
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4645 4826 4989 4645
Size: 181 163 6351
Fragments arranged by size:
6351 181 163
BtsI GCAGTG_nn'
Cuts at: 370 2654 2913 3938 370
Size: 2284 259 1025 3127
Fragments arranged by size:
3127 2284 1025 259
ClaI AT'CG_AT
Cuts at: 428 2093 428
Size: 1665 5030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2286 2321 2286
Size: 35 6660
DraI TTT'AAA
Cuts at: 3694 3694
Size: 6695
DraIII CAC_nnn'GTG
Cuts at: 1461 1461
Size: 6695
DrdI GACnn_nn'nnGTC
Cuts at: 328 936 2394 4401 5259 328
Size: 608 1458 2007 858 1764
Fragments arranged by size:
2007 1764 1458 858 608
EaeI y'GGCC_r
Cuts at: 854 1988 2294 2756 3167 4280 4454 4845 4872
Size: 1134 306 462 411 1113 174 391 27
Cuts at: 4872 6217 854
Size: 1345 1332
Fragments arranged by size:
1345 1332 1134 1113 462 411 391 306
174 27
EagI C'GGCC_G
Cuts at: 4280 4280
Size: 6695
EarI CTCTTCn'nnn_
Cuts at: 288 668 686 1039 1711 4718 4928 288
Size: 380 18 353 672 3007 210 2055
Fragments arranged by size:
3007 2055 672 380 353 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3102 4903 5213 5359 6505 3102
Size: 1801 310 146 1146 3292
Fragments arranged by size:
3292 1801 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3707 3707
Size: 6695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1543 1777 2155 2252 2404 2425 2527 2656
Size: 480 234 378 97 152 21 102 129
Cuts at: 2656 2823 3013 3511 3541 4520 4952 5699 5967
Size: 167 190 498 30 979 432 747 268
Cuts at: 5967 1063
Size: 1791
Fragments arranged by size:
1791 979 747 498 480 432 378 268
234 190 167 152 129 102 97 30
21
EcoNI CCTnn'n_nnAGG
Cuts at: 209 1298 1831 2173 2617 3190 209
Size: 1089 533 342 444 573 3714
Fragments arranged by size:
3714 1089 573 533 444 342
EcoO109I rG'GnC_Cy
Cuts at: 240 490 637 645 1279 1398 1630 1697 1698
Size: 250 147 8 634 119 232 67 1
Cuts at: 1698 1836 2178 2832 3537 3649 3834 240
Size: 138 342 654 705 112 185 3101
Fragments arranged by size:
3101 705 654 634 342 250 232 185
147 138 119 112 67 8 1
EcoRI G'AATT_C
Cuts at: 3699 3699
Size: 6695
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2307 4714 2307
Size: 2407 4288
FseI GG_CCGG'CC
Cuts at: 3173 3173
Size: 6695
FspI TGC'GCA
Cuts at: 2052 2281 2545 3568 3673 4476 2052
Size: 229 264 1023 105 803 4271
Fragments arranged by size:
4271 1023 803 264 229 105
FspAI rTGC'GCAy
Cuts at: 2052 2052
Size: 6695
HaeII r_GCGC'y
Cuts at: 993 1137 1530 1902 2169 2445 2727 3450 4129
Size: 144 393 372 267 276 282 723 679
Cuts at: 4129 4377 5399 6378 993
Size: 248 1022 979 1310
Fragments arranged by size:
1310 1022 979 723 679 393 372 282
276 267 248 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 206 210 238 242 715 747 1370 1402 2599
Size: 4 28 4 473 32 623 32 1197
Cuts at: 2599 2631 2959 2991 4537 4567 4569 4599 206
Size: 32 328 32 1546 30 2 30 2302
Fragments arranged by size:
2302 1546 1197 623 473 328 32 32
32 32 30 30 28 4 4 2
HincII GTy'rAC
Cuts at: 201 3287 3734 201
Size: 3086 447 3162
Fragments arranged by size:
3162 3086 447
KasI G'GCGC_C
Cuts at: 989 3446 4125 4373 989
Size: 2457 679 248 3311
Fragments arranged by size:
3311 2457 679 248
KpnI G_GTAC'C
Cuts at: 3715 3715
Size: 6695
MluI A'CGCG_T
Cuts at: 6128 6128
Size: 6695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1304 1649 2249 5366 5550 1304
Size: 345 600 3117 184 2449
Fragments arranged by size:
3117 2449 600 345 184
MscI TGG'CCA
Cuts at: 856 1990 2296 2758 4456 856
Size: 1134 306 462 1698 3095
Fragments arranged by size:
3095 1698 1134 462 306
MslI CAynn'nnrTG
Cuts at: 192 344 1153 1936 2093 2233 3193 3434 4684
Size: 152 809 783 157 140 960 241 1250
Cuts at: 4684 4811 192
Size: 127 2076
Fragments arranged by size:
2076 1250 960 809 783 241 157 152
140 127
MspA1I CmG'CkG
Cuts at: 451 465 563 609 1515 1556 1611 1845 2462
Size: 14 98 46 906 41 55 234 617
Cuts at: 2462 2697 2903 3618 3682 3806 4120 4480 5493
Size: 235 206 715 64 124 314 360 1013
Cuts at: 5493 5738 451
Size: 245 1408
Fragments arranged by size:
1408 1013 906 715 617 360 314 245
235 234 206 124 98 64 55 46
41 14
NaeI GCC'GGC
Cuts at: 2503 2940 3171 4876 2503
Size: 437 231 1705 4322
Fragments arranged by size:
4322 1705 437 231
NarI GG'CG_CC
Cuts at: 990 3447 4126 4374 990
Size: 2457 679 248 3311
Fragments arranged by size:
3311 2457 679 248
NgoMIV G'CCGG_C
Cuts at: 2501 2938 3169 4874 2501
Size: 437 231 1705 4322
Fragments arranged by size:
4322 1705 437 231
NheI G'CTAG_C
Cuts at: 531 3396 531
Size: 2865 3830
NspI r_CATG'y
Cuts at: 316 2190 2344 2589 3748 4248 5155 316
Size: 1874 154 245 1159 500 907 1856
Fragments arranged by size:
1874 1856 1159 907 500 245 154
PasI CC'CwG_GG
Cuts at: 2181 2715 3540 2181
Size: 534 825 5336
Fragments arranged by size:
5336 825 534
PciI A'CATG_T
Cuts at: 5151 5151
Size: 6695
PflMI CCAn_nnn'nTGG
Cuts at: 918 1626 918
Size: 708 5987
PfoI T'CCnGG_A
Cuts at: 2171 2672 3371 2171
Size: 501 699 5495
Fragments arranged by size:
5495 699 501
PmeI GTTT'AAAC
Cuts at: 3694 3694
Size: 6695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5834 5866 5834
Size: 32 6663
PpuMI rG'GwC_Cy
Cuts at: 240 490 637 2832 3649 240
Size: 250 147 2195 817 3286
Fragments arranged by size:
3286 2195 817 250 147
PspOMI G'GGCC_C
Cuts at: 140 1629 1697 3536 140
Size: 1489 68 1839 3299
Fragments arranged by size:
3299 1839 1489 68
PstI C_TGCA'G
Cuts at: 450 456 1244 1742 1844 3742 4427 450
Size: 6 788 498 102 1898 685 2718
Fragments arranged by size:
2718 1898 788 685 498 102 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6695
PvuII CAG'CTG
Cuts at: 451 465 563 609 1515 3618 3682 4120 4480
Size: 14 98 46 906 2103 64 438 360
Cuts at: 4480 451
Size: 2666
Fragments arranged by size:
2666 2103 906 438 360 98 64 46
14
RsrII CG'GwC_CG
Cuts at: 4890 4890
Size: 6695
SacI G_AGCT'C
Cuts at: 3709 3709
Size: 6695
SalI G'TCGA_C
Cuts at: 3285 3732 3285
Size: 447 6248
SanDI GG'GwC_CC
Cuts at: 2832 2832
Size: 6695
SapI GCTCTTCn'nnn_
Cuts at: 288 686 1039 1711 4718 4928 288
Size: 398 353 672 3007 210 2055
Fragments arranged by size:
3007 2055 672 398 353 210
SbfI CC_TGCA'GG
Cuts at: 3742 3742
Size: 6695
ScaI AGT'ACT
Cuts at: 6212 6212
Size: 6695
SexAI A'CCwGG_T
Cuts at: 2109 2561 2109
Size: 452 6243
SfcI C'TryA_G
Cuts at: 33 446 452 1240 1738 1840 2851 3738 4423
Size: 413 6 788 498 102 1011 887 685
Cuts at: 4423 5416 5607 33
Size: 993 191 1121
Fragments arranged by size:
1121 1011 993 887 788 685 498 413
191 102 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 2456 2456
Size: 6695
SfoI GGC'GCC
Cuts at: 991 3448 4127 4375 991
Size: 2457 679 248 3311
Fragments arranged by size:
3311 2457 679 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6695
SmaI CCC'GGG
Cuts at: 2830 2980 3717 2830
Size: 150 737 5808
Fragments arranged by size:
5808 737 150
SmlI C'TyrA_G
Cuts at: 227 771 1831 2554 3850 4765 5257 5519 227
Size: 544 1060 723 1296 915 492 262 1403
Fragments arranged by size:
1403 1296 1060 915 723 544 492 262
SpeI A'CTAG_T
Cuts at: 67 327 67
Size: 260 6435
SphI G_CATG'C
Cuts at: 2190 3748 4248 2190
Size: 1558 500 4637
Fragments arranged by size:
4637 1558 500
StuI AGG'CCT
Cuts at: 3073 3073
Size: 6695
StyI C'CwwG_G
Cuts at: 857 1025 1262 2390 2618 2759 3829 4183 857
Size: 168 237 1128 228 141 1070 354 3369
Fragments arranged by size:
3369 1128 1070 354 237 228 168 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 204 366 1111 1552 1588 2126 2813 3030 3430
Size: 162 745 441 36 538 687 217 400
Cuts at: 3430 4278 4411 4870 5079 204
Size: 848 133 459 209 1820
Fragments arranged by size:
1820 848 745 687 538 459 441 400
217 209 162 133 36
TatI w'GTAC_w
Cuts at: 3128 6210 3128
Size: 3082 3613
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2398 2430 6212 6244 2398
Size: 32 3782 32 2849
Fragments arranged by size:
3782 2849 32 32
Tth111I GACn'n_nGTC
Cuts at: 4492 4492
Size: 6695
XbaI T'CTAG_A
Cuts at: 3726 3726
Size: 6695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 849 1098 4190 114
Size: 735 249 3092 2619
Fragments arranged by size:
3092 2619 735 249
XhoI C'TCGA_G
Cuts at: 227 227
Size: 6695
XmaI C'CCGG_G
Cuts at: 2828 2978 3715 2828
Size: 150 737 5808
Fragments arranged by size:
5808 737 150
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI AscI AvrII BbvCI BfrBI
Bpu10I BsiWI BsrGI BssHII BstZ17I Bsu36I EcoRV HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI