Restriction map for
pF1KA0896
(Circular) (Six-base) MAPSORT of: pF1KA0896.seq Check: 1665 from: 1 to: 11507
pF1KA0896 11507 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1564 1564
Size: 11507
AatII G_ACGT'C
Cuts at: 115 412 115
Size: 297 11210
AccI GT'mk_AC
Cuts at: 2325 2772 3114 4214 5534 6210 7508 8076 8545
Size: 447 342 1100 1320 676 1298 568 469
Cuts at: 8545 2325
Size: 5287
Fragments arranged by size:
5287 1320 1298 1100 676 568 469 447
342
Acc65I G'GTAC_C
Cuts at: 1699 2557 3592 5374 8523 1699
Size: 858 1035 1782 3149 4683
Fragments arranged by size:
4683 3149 1782 1035 858
AclI AA'CG_TT
Cuts at: 11347 11347
Size: 11507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 718 944 1904 2407 4055 5114 5675 6263 7265
Size: 226 960 503 1648 1059 561 588 1002
Cuts at: 7265 9332 9764 10511 10779 718
Size: 2067 432 747 268 1446
Fragments arranged by size:
2067 1648 1446 1059 1002 960 747 588
561 503 432 268 226
AflIII A'CryG_T
Cuts at: 1954 7761 8859 9963 10940 1954
Size: 5807 1098 1104 977 2521
Fragments arranged by size:
5807 2521 1104 1098 977
AgeI A'CCGG_T
Cuts at: 3271 9895 3271
Size: 6624 4883
AhdI GACnn_n'nnGTC
Cuts at: 8422 8422
Size: 11507
AleI CACnn'nnGTG
Cuts at: 890 2852 890
Size: 1962 9545
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 6302 7823 8421 6302
Size: 1521 598 9388
Fragments arranged by size:
9388 1521 598
AlwNI CAG_nnn'CTG
Cuts at: 457 655 2203 2829 4966 5047 5407 5554 6154
Size: 198 1548 626 2137 81 360 147 600
Cuts at: 6154 6730 7243 7876 8812 10379 457
Size: 576 513 633 936 1567 1585
Fragments arranged by size:
2137 1585 1567 1548 936 633 626 600
576 513 360 198 147 81
ApaLI G'TGCA_C
Cuts at: 10277 10277
Size: 11507
ApoI r'AATT_y
Cuts at: 1216 2149 5736 6778 8097 8487 8511 10860 11116
Size: 933 3587 1042 1319 390 24 2349 256
Cuts at: 11116 1216
Size: 1607
Fragments arranged by size:
3587 2349 1607 1319 1042 933 390 256
24
AseI AT'TA_AT
Cuts at: 20 20
Size: 11507
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 11507
AvaI C'yCGr_G
Cuts at: 393 560 581 989 8527 393
Size: 167 21 408 7538 3373
Fragments arranged by size:
7538 3373 408 167 21
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1691 1724 4307 4340 1691
Size: 33 2583 33 8858
Fragments arranged by size:
8858 2583 33 33
BamHI G'GATC_C
Cuts at: 532 2601 3150 3207 8532 532
Size: 2069 549 57 5325 3507
Fragments arranged by size:
5325 3507 2069 549 57
BanI G'GyrC_C
Cuts at: 145 1699 2131 2557 3592 5374 6674 7069 8523
Size: 1554 432 426 1035 1782 1300 395 1454
Cuts at: 8523 8937 9185 9220 145
Size: 414 248 35 2432
Fragments arranged by size:
2432 1782 1554 1454 1300 1035 432 426
414 395 248 35
BanII G_rGCy'C
Cuts at: 561 1236 8521 9551 561
Size: 675 7285 1030 2517
Fragments arranged by size:
7285 2517 1030 675
BbeI G_GCGC'C
Cuts at: 6678 8941 9189 6678
Size: 2263 248 8996
Fragments arranged by size:
8996 2263 248
BbsI GAAGACnn'nnnn_
Cuts at: 932 2395 4934 7209 932
Size: 1463 2539 2275 5230
Fragments arranged by size:
5230 2539 2275 1463
BbvCI CC'TCA_GC
Cuts at: 4895 4895
Size: 11507
BciVI GTATCCnnnnn_n'
Cuts at: 6003 7060 7498 9405 10166 11507 6003
Size: 1057 438 1907 761 1341 6003
Fragments arranged by size:
6003 1907 1341 1057 761 438
BclI T'GATC_A
Cuts at: 4683 6261 8174 9027 4683
Size: 1578 1913 853 7163
Fragments arranged by size:
7163 1913 1578 853
BfrBI ATG'CAT
Cuts at: 1966 1966
Size: 11507
BglII A'GATC_T
Cuts at: 1837 6852 8286 9022 11059 1837
Size: 5015 1434 736 2037 2285
Fragments arranged by size:
5015 2285 2037 1434 736
BlpI GC'TnA_GC
Cuts at: 1562 1889 5818 7385 8619 1562
Size: 327 3929 1567 1234 4450
Fragments arranged by size:
4450 3929 1567 1234 327
Bme1580I G_kGCm'C
Cuts at: 148 1548 9132 9225 10281 148
Size: 1400 7584 93 1056 1374
Fragments arranged by size:
7584 1400 1374 1056 93
BmgBI CAC'GTC
Cuts at: 5806 5806
Size: 11507
BmrI ACTGGGnnnn_n'
Cuts at: 6064 8719 8800 9135 11279 6064
Size: 2655 81 335 2144 6292
Fragments arranged by size:
6292 2655 2144 335 81
BmtI G_CTAG'C
Cuts at: 1874 5423 5780 1874
Size: 3549 357 7601
Fragments arranged by size:
7601 3549 357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1035 3946 6874 9874 1035
Size: 2911 2928 3000 2668
Fragments arranged by size:
3000 2928 2911 2668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 530 575 860 1760 1928 2148 3146 3385
Size: 8 45 285 900 168 220 998 239
Cuts at: 3385 3680 3733 4683 4865 522
Size: 295 53 950 182 7164
Fragments arranged by size:
7164 998 950 900 295 285 239 220
182 168 53 45 8
Bpu10I CC'TnA_GC
Cuts at: 4895 7268 4895
Size: 2373 9134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 1734 2812 3528 5103 8683 9562 10054 10352
Size: 1421 1078 716 1575 3580 879 492 298
Cuts at: 10352 313
Size: 1468
Fragments arranged by size:
3580 1575 1468 1421 1078 879 716 492
298
BsaI GGTCTCn'nnnn_
Cuts at: 2066 5161 5891 7109 11211 2066
Size: 3095 730 1218 4102 2362
Fragments arranged by size:
4102 3095 2362 1218 730
BsaAI yAC'GTr
Cuts at: 1060 1955 8860 1060
Size: 895 6905 3707
Fragments arranged by size:
6905 3707 895
BsaBI GATnn'nnATC
Cuts at: 6 2861 6845 6851 9021 11058 6
Size: 2855 3984 6 2170 2037 455
Fragments arranged by size:
3984 2855 2170 2037 455 6
BsaHI Gr'CG_yC
Cuts at: 112 409 6675 8938 9186 9888 11384 112
Size: 297 6266 2263 248 702 1496 235
Fragments arranged by size:
6266 2263 1496 702 297 248 235
BsaWI w'CCGG_w
Cuts at: 3271 8696 9217 9895 10169 10316 10727 3271
Size: 5425 521 678 274 147 411 4051
Fragments arranged by size:
5425 4051 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 3706 3736 7140 7143 7170 7173 7931 7961 3706
Size: 30 3404 3 27 3 758 30 7252
Fragments arranged by size:
7252 3404 758 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 682 5864 7121 7161 7164 7503 682
Size: 5182 1257 40 3 339 4686
Fragments arranged by size:
5182 4686 1257 339 40 3
BseYI C'CCAG_C
Cuts at: 635 1765 4082 5270 7380 8932 9690 10267 10763
Size: 1130 2317 1188 2110 1552 758 577 496
Cuts at: 10763 635
Size: 1379
Fragments arranged by size:
2317 2110 1552 1379 1188 1130 758 577
496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2006 4574 5828 6527 2006
Size: 2568 1254 699 6986
Fragments arranged by size:
6986 2568 1254 699
BsiEI CG_ry'CG
Cuts at: 104 9049 9095 10303 104
Size: 8945 46 1208 1308
Fragments arranged by size:
8945 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 561 1487 3671 3929 8521 9299 9489 10281 561
Size: 926 2184 258 4592 778 190 792 1787
Fragments arranged by size:
4592 2184 1787 926 792 778 258 190
BsmI GAATG_Cn'
Cuts at: 2652 3363 4122 5901 2652
Size: 711 759 1779 8258
Fragments arranged by size:
8258 1779 759 711
BsmBI CGTCTCn'nnnn_
Cuts at: 3078 7865 3078
Size: 4787 6720
Bsp1286I G_dGCh'C
Cuts at: 148 561 1236 1487 1548 3671 3929 8521 9132
Size: 413 675 251 61 2123 258 4592 611
Cuts at: 9132 9225 9299 9489 9551 10281 148
Size: 93 74 190 62 730 1374
Fragments arranged by size:
4592 2123 1374 730 675 611 413 258
251 190 93 74 62 61
BspEI T'CCGG_A
Cuts at: 10727 10727
Size: 11507
BspHI T'CATG_A
Cuts at: 3762 4056 4860 6531 10683 3762
Size: 294 804 1671 4152 4586
Fragments arranged by size:
4586 4152 1671 804 294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1564 2247 7159 8557 8771 9073 9454 1564
Size: 683 4912 1398 214 302 381 3617
Fragments arranged by size:
4912 3617 1398 683 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1521 9799 9853 11337 11502 50
Size: 1471 8278 54 1484 165 55
Fragments arranged by size:
8278 1484 1471 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1536 2471 2990 4260 4504 5993 7001 7334 7901
Size: 935 519 1270 244 1489 1008 333 567
Cuts at: 7901 8069 9419 1536
Size: 168 1350 3624
Fragments arranged by size:
3624 1489 1350 1270 1008 935 567 519
333 244 168
BsrFI r'CCGG_y
Cuts at: 572 3271 6455 9505 9686 9895 572
Size: 2699 3184 3050 181 209 2184
Fragments arranged by size:
3184 3050 2699 2184 209 181
BsrGI T'GTAC_A
Cuts at: 4279 6927 7320 4279
Size: 2648 393 8466
Fragments arranged by size:
8466 2648 393
BssSI C'ACGA_G
Cuts at: 1343 5846 7316 9778 10136 1343
Size: 4503 1470 2462 358 2714
Fragments arranged by size:
4503 2714 2462 1470 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5047 5407 8884 5047
Size: 360 3477 7670
Fragments arranged by size:
7670 3477 360
BstBI TT'CG_AA
Cuts at: 9868 9868
Size: 11507
BstEII G'GTnAC_C
Cuts at: 943 943
Size: 11507
BstXI CCAn_nnnn'nTGG
Cuts at: 5221 6255 5221
Size: 1034 10473
BstYI r'GATC_y
Cuts at: 1 532 846 1837 2601 2616 3150 3207 3250
Size: 531 314 991 764 15 534 57 43
Cuts at: 3250 6300 6852 7494 8286 8532 8999 9022 9357
Size: 3050 552 642 792 246 467 23 335
Cuts at: 9357 9603 10615 10701 10713 11059 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3050 1012 991 792 764 642 552 534
531 467 449 346 335 314 246 246
86 57 43 23 15 12
BstZ17I GTA'TAC
Cuts at: 2326 4215 5535 6211 2326
Size: 1889 1320 676 7622
Fragments arranged by size:
7622 1889 1320 676
Bsu36I CC'TnA_GG
Cuts at: 489 489
Size: 11507
BtgI C'CryG_G
Cuts at: 439 7442 7545 439
Size: 7003 103 4401
Fragments arranged by size:
7003 4401 103
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 7274 9457 9638 9801 3217
Size: 4057 2183 181 163 4923
Fragments arranged by size:
4923 4057 2183 181 163
BtsI GCAGTG_nn'
Cuts at: 1195 1487 1989 2062 2357 2513 3884 4641 5036
Size: 292 502 73 295 156 1371 757 395
Cuts at: 5036 5079 5257 6978 8317 8750 1195
Size: 43 178 1721 1339 433 3952
Fragments arranged by size:
3952 1721 1371 1339 757 502 433 395
295 292 178 156 73 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2253 2288 6257 6292 2253
Size: 35 3969 35 7468
Fragments arranged by size:
7468 3969 35 35
DraI TTT'AAA
Cuts at: 209 8506 209
Size: 8297 3210
DrdI GACnn_nn'nnGTC
Cuts at: 9213 10071 9213
Size: 858 10649
EaeI y'GGCC_r
Cuts at: 2573 3474 6156 6309 9092 9266 9657 9684 11029
Size: 901 2682 153 2783 174 391 27 1345
Cuts at: 11029 2573
Size: 3051
Fragments arranged by size:
3051 2783 2682 1345 901 391 174 153
27
EagI C'GGCC_G
Cuts at: 9092 9092
Size: 11507
EarI CTCTTCn'nnn_
Cuts at: 2027 2045 2069 2924 4199 4811 4844 5726 5742
Size: 18 24 855 1275 612 33 882 16
Cuts at: 5742 6146 9530 9740 2027
Size: 404 3384 210 3794
Fragments arranged by size:
3794 3384 1275 882 855 612 404 210
33 24 18 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 9715 10025 10171 11317 9715
Size: 310 146 1146 9905
Fragments arranged by size:
9905 1146 310 146
EcoICRI GAG'CTC
Cuts at: 559 8519 559
Size: 7960 3547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 530 575 718 860 944 1760 1904 1928
Size: 8 45 143 142 84 816 144 24
Cuts at: 1928 2148 2407 3146 3385 3680 3733 4055 4683
Size: 220 259 739 239 295 53 322 628
Cuts at: 4683 4865 5114 5675 6263 7265 9332 9764 10511
Size: 182 249 561 588 1002 2067 432 747
Cuts at: 10511 10779 522
Size: 268 1250
Fragments arranged by size:
2067 1250 1002 816 747 739 628 588
561 432 322 295 268 259 249 239
220 182 144 143 142 84 53 45
24 8
EcoNI CCTnn'n_nnAGG
Cuts at: 540 540
Size: 11507
EcoO109I rG'GnC_Cy
Cuts at: 1930 3310 7226 8646 1930
Size: 1380 3916 1420 4791
Fragments arranged by size:
4791 3916 1420 1380
EcoRI G'AATT_C
Cuts at: 5736 8511 5736
Size: 2775 8732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1291 4183 5626 6131 7795 9526 1291
Size: 2892 1443 505 1664 1731 3272
Fragments arranged by size:
3272 2892 1731 1664 1443 505
FspI TGC'GCA
Cuts at: 9288 9288
Size: 11507
HaeII r_GCGC'y
Cuts at: 5825 6678 7545 8941 9189 10211 11190 5825
Size: 853 867 1396 248 1022 979 6142
Fragments arranged by size:
6142 1396 1022 979 867 853 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 595 627 729 761 838 870 1069 1101 1707
Size: 32 102 32 77 32 199 32 606
Cuts at: 1707 1739 1954 1986 2044 2076 2399 2431 4566
Size: 32 215 32 58 32 323 32 2135
Cuts at: 4566 4598 5092 5124 5917 5949 6121 6153 6926
Size: 32 494 32 793 32 172 32 773
Cuts at: 6926 6958 7296 7328 8058 8090 8408 8440 9349
Size: 32 338 32 730 32 318 32 909
Cuts at: 9349 9379 9381 9411 595
Size: 30 2 30 2691
Fragments arranged by size:
2691 2135 909 793 773 730 606 494
338 323 318 215 199 172 102 77
58 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1364 2015 2773 8546 1364
Size: 651 758 5773 4325
Fragments arranged by size:
5773 4325 758 651
HindIII A'AGCT_T
Cuts at: 1848 2317 5608 8221 1848
Size: 469 3291 2613 5134
Fragments arranged by size:
5134 3291 2613 469
HpaI GTT'AAC
Cuts at: 1364 1364
Size: 11507
KasI G'GCGC_C
Cuts at: 6674 8937 9185 6674
Size: 2263 248 8996
Fragments arranged by size:
8996 2263 248
KpnI G_GTAC'C
Cuts at: 1703 2561 3596 5378 8527 1703
Size: 858 1035 1782 3149 4683
Fragments arranged by size:
4683 3149 1782 1035 858
MfeI C'AATT_G
Cuts at: 1973 4475 7794 7913 1973
Size: 2502 3319 119 5567
Fragments arranged by size:
5567 3319 2502 119
MluI A'CGCG_T
Cuts at: 10940 10940
Size: 11507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2926 4609 6201 6427 6699 7349 10178 10362 2926
Size: 1683 1592 226 272 650 2829 184 4071
Fragments arranged by size:
4071 2829 1683 1592 650 272 226 184
MscI TGG'CCA
Cuts at: 2575 6158 9268 2575
Size: 3583 3110 4814
Fragments arranged by size:
4814 3583 3110
MslI CAynn'nnrTG
Cuts at: 890 1541 1953 2852 2882 3574 4862 5219 5894
Size: 651 412 899 30 692 1288 357 675
Cuts at: 5894 5906 6086 7360 9496 9623 890
Size: 12 180 1274 2136 127 2774
Fragments arranged by size:
2774 2136 1288 1274 899 692 675 651
412 357 180 127 30 12
MspA1I CmG'CkG
Cuts at: 138 500 553 1566 1820 3103 3244 3466 4966
Size: 362 53 1013 254 1283 141 222 1500
Cuts at: 4966 5332 5398 5407 5554 7389 8618 8932 9292
Size: 366 66 9 147 1835 1229 314 360
Cuts at: 9292 10305 10550 138
Size: 1013 245 1095
Fragments arranged by size:
1835 1500 1283 1229 1095 1013 1013 366
362 360 314 254 245 222 147 141
66 53 9
NaeI GCC'GGC
Cuts at: 574 9688 574
Size: 9114 2393
NarI GG'CG_CC
Cuts at: 6675 8938 9186 6675
Size: 2263 248 8996
Fragments arranged by size:
8996 2263 248
NcoI C'CATG_G
Cuts at: 439 7442 7545 439
Size: 7003 103 4401
Fragments arranged by size:
7003 4401 103
NdeI CA'TA_TG
Cuts at: 5952 5952
Size: 11507
NgoMIV G'CCGG_C
Cuts at: 572 9686 572
Size: 9114 2393
NheI G'CTAG_C
Cuts at: 1870 5419 5776 1870
Size: 3549 357 7601
Fragments arranged by size:
7601 3549 357
NsiI A_TGCA'T
Cuts at: 1968 1968
Size: 11507
NspI r_CATG'y
Cuts at: 2881 5947 6450 7365 7765 8062 8560 9060 9967
Size: 3066 503 915 400 297 498 500 907
Cuts at: 9967 2881
Size: 4421
Fragments arranged by size:
4421 3066 915 907 503 500 498 400
297
PciI A'CATG_T
Cuts at: 7761 9963 7761
Size: 2202 9305
PflMI CCAn_nnn'nTGG
Cuts at: 126 7657 126
Size: 7531 3976
PfoI T'CCnGG_A
Cuts at: 2154 5700 7490 2154
Size: 3546 1790 6171
Fragments arranged by size:
6171 3546 1790
PmeI GTTT'AAAC
Cuts at: 8506 8506
Size: 11507
PmlI CAC'GTG
Cuts at: 1955 1955
Size: 11507
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 408 10646 10678 376
Size: 32 10238 32 1205
Fragments arranged by size:
10238 1205 32 32
PpuMI rG'GwC_Cy
Cuts at: 1930 3310 1930
Size: 1380 10127
PshAI GACnn'nnGTC
Cuts at: 2010 2010
Size: 11507
PspXI vC'TCGA_Gb
Cuts at: 581 581
Size: 11507
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8039 8071 8039
Size: 32 11475
PstI C_TGCA'G
Cuts at: 2225 2834 5043 5397 5403 6150 8231 8554 9239
Size: 609 2209 354 6 747 2081 323 685
Cuts at: 9239 2225
Size: 4493
Fragments arranged by size:
4493 2209 2081 747 685 609 354 323
6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 11507
PvuII CAG'CTG
Cuts at: 553 1566 1820 3103 3466 4966 5398 5407 5554
Size: 1013 254 1283 363 1500 432 9 147
Cuts at: 5554 7389 8932 9292 553
Size: 1835 1543 360 2768
Fragments arranged by size:
2768 1835 1543 1500 1283 1013 432 363
360 254 147 9
RsrII CG'GwC_CG
Cuts at: 9702 9702
Size: 11507
SacI G_AGCT'C
Cuts at: 561 8521 561
Size: 7960 3547
SalI G'TCGA_C
Cuts at: 2771 8544 2771
Size: 5773 5734
SapI GCTCTTCn'nnn_
Cuts at: 2045 4199 9530 9740 2045
Size: 2154 5331 210 3812
Fragments arranged by size:
5331 3812 2154 210
SbfI CC_TGCA'GG
Cuts at: 2225 8554 2225
Size: 6329 5178
ScaI AGT'ACT
Cuts at: 4611 11024 4611
Size: 6413 5094
SfcI C'TryA_G
Cuts at: 33 1412 1418 1821 2221 2429 2717 2821 2830
Size: 1379 6 403 400 208 288 104 9
Cuts at: 2830 3329 4105 5039 5393 5399 6146 8227 8550
Size: 499 776 934 354 6 747 2081 323
Cuts at: 8550 9235 10228 10419 33
Size: 685 993 191 1121
Fragments arranged by size:
2081 1379 1121 993 934 776 747 685
499 403 400 354 323 288 208 191
104 9 6 6
SfoI GGC'GCC
Cuts at: 6676 8939 9187 6676
Size: 2263 248 8996
Fragments arranged by size:
8996 2263 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 11507
SmaI CCC'GGG
Cuts at: 8529 8529
Size: 11507
SmlI C'TyrA_G
Cuts at: 292 560 581 989 1713 2791 3543 5118 8662
Size: 268 21 408 724 1078 752 1575 3544
Cuts at: 8662 9577 10069 10331 292
Size: 915 492 262 1468
Fragments arranged by size:
3544 1575 1468 1078 915 752 724 492
408 268 262 21
SpeI A'CTAG_T
Cuts at: 67 4320 67
Size: 4253 7254
SphI G_CATG'C
Cuts at: 2881 8560 9060 2881
Size: 5679 500 5328
Fragments arranged by size:
5679 5328 500
SspI AAT'ATT
Cuts at: 2345 2871 2345
Size: 526 10981
StuI AGG'CCT
Cuts at: 417 2767 7656 417
Size: 2350 4889 4268
Fragments arranged by size:
4889 4268 2350
StyI C'CwwG_G
Cuts at: 439 2481 3341 3431 6201 6254 7442 7545 7651
Size: 2042 860 90 2770 53 1188 103 106
Cuts at: 7651 8641 8995 439
Size: 990 354 2951
Fragments arranged by size:
2951 2770 2042 1188 990 860 354 106
103 90 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 505 902 1434 2635 5284 6194 6742 7087 9090
Size: 397 532 1201 2649 910 548 345 2003
Cuts at: 9090 9223 9682 9891 505
Size: 133 459 209 2121
Fragments arranged by size:
2649 2121 2003 1201 910 548 532 459
397 345 209 133
TatI w'GTAC_w
Cuts at: 327 3945 4279 4609 5290 5305 5383 6927 7320
Size: 3618 334 330 681 15 78 1544 393
Cuts at: 7320 11022 327
Size: 3702 812
Fragments arranged by size:
3702 3618 1544 812 681 393 334 330
78 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1278 1310 2633 2665 3706 3738 4516 4548 7140
Size: 32 1323 32 1041 32 778 32 2592
Cuts at: 7140 7172 11024 11056 1278
Size: 32 3852 32 1729
Fragments arranged by size:
3852 2592 1729 1323 1041 778 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 7718 9304 7718
Size: 1586 9921
XbaI T'CTAG_A
Cuts at: 7041 8538 7041
Size: 1497 10010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 123 2568 2583 2854 9002 123
Size: 2445 15 271 6148 2628
Fragments arranged by size:
6148 2628 2445 271 15
XhoI C'TCGA_G
Cuts at: 560 581 989 560
Size: 21 408 11078
Fragments arranged by size:
11078 408 21
XmaI C'CCGG_G
Cuts at: 8527 8527
Size: 11507
XmnI GAAnn'nnTTC
Cuts at: 7687 7955 7687
Size: 268 11239
ZraI GAC'GTC
Cuts at: 113 410 113
Size: 297 11210
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AloI ApaI AscI AvrII BcgI BglI
BsiWI BssHII ClaI DraIII EcoRV FseI FspAI NotI
NruI PacI PasI PsiI PspOMI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI