Restriction map for
pF1KA0901
(Circular) (Six-base) MAPSORT of: pF1KA0901.seq Check: 8384 from: 1 to: 6758
pF1KA0901 6758 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1220 2408 1220
Size: 1188 5570
AccI GT'mk_AC
Cuts at: 3560 3796 3560
Size: 236 6522
Acc65I G'GTAC_C
Cuts at: 1614 3774 1614
Size: 2160 4598
AclI AA'CG_TT
Cuts at: 6598 6598
Size: 6758
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 988 1304 1358 4583 5015 5762 6030 368
Size: 620 316 54 3225 432 747 268 1096
Fragments arranged by size:
3225 1096 747 620 432 316 268 54
AflIII A'CryG_T
Cuts at: 2081 3750 4110 5214 6191 2081
Size: 1669 360 1104 977 2648
Fragments arranged by size:
2648 1669 1104 977 360
AgeI A'CCGG_T
Cuts at: 5146 5146
Size: 6758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 587 619 634 666 587
Size: 32 15 32 6679
Fragments arranged by size:
6679 32 32 15
AlwNI CAG_nnn'CTG
Cuts at: 676 1708 2597 4063 5630 676
Size: 1032 889 1466 1567 1804
Fragments arranged by size:
1804 1567 1466 1032 889
ApaI G_GGCC'C
Cuts at: 2231 2556 2231
Size: 325 6433
ApaLI G'TGCA_C
Cuts at: 868 1841 5528 868
Size: 973 3687 2098
Fragments arranged by size:
3687 2098 973
ApoI r'AATT_y
Cuts at: 394 3606 3762 6111 6367 394
Size: 3212 156 2349 256 785
Fragments arranged by size:
3212 2349 785 256 156
AseI AT'TA_AT
Cuts at: 20 20
Size: 6758
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
AvaI C'yCGr_G
Cuts at: 1102 1775 3180 3778 1102
Size: 673 1405 598 4082
Fragments arranged by size:
4082 1405 673 598
AvrII C'CTAG_G
Cuts at: 2689 2689
Size: 6758
BamHI G'GATC_C
Cuts at: 2033 2346 3783 2033
Size: 313 1437 5008
Fragments arranged by size:
5008 1437 313
BanI G'GyrC_C
Cuts at: 1348 1614 2137 2164 2670 3151 3774 4188 4436
Size: 266 523 27 506 481 623 414 248
Cuts at: 4436 4471 1348
Size: 35 3635
Fragments arranged by size:
3635 623 523 506 481 414 266 248
35 27
BanII G_rGCy'C
Cuts at: 1406 1505 2231 2556 3772 4802 1406
Size: 99 726 325 1216 1030 3362
Fragments arranged by size:
3362 1216 1030 726 325 99
BbeI G_GCGC'C
Cuts at: 4192 4440 4192
Size: 248 6510
BbsI GAAGACnn'nnnn_
Cuts at: 308 2633 308
Size: 2325 4433
BbvCI CC'TCA_GC
Cuts at: 1115 3628 1115
Size: 2513 4245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2160 2194 3363 3397 2160
Size: 34 1169 34 5521
Fragments arranged by size:
5521 1169 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4656 5417 6758 4656
Size: 761 1341 4656
Fragments arranged by size:
4656 1341 761
BclI T'GATC_A
Cuts at: 2130 3405 4278 2130
Size: 1275 873 4610
Fragments arranged by size:
4610 1275 873
BglI GCCn_nnn'nGGC
Cuts at: 664 1186 1705 2180 2866 664
Size: 522 519 475 686 4556
Fragments arranged by size:
4556 686 522 519 475
BglII A'GATC_T
Cuts at: 4273 6310 4273
Size: 2037 4721
BlpI GC'TnA_GC
Cuts at: 1709 2699 3870 1709
Size: 990 1171 4597
Fragments arranged by size:
4597 1171 990
Bme1580I G_kGCm'C
Cuts at: 872 1353 1368 1845 2197 2231 2556 3154 4383
Size: 481 15 477 352 34 325 598 1229
Cuts at: 4383 4476 5532 872
Size: 93 1056 2098
Fragments arranged by size:
2098 1229 1056 598 481 477 352 325
93 34 15
BmgBI CAC'GTC
Cuts at: 1083 3492 1083
Size: 2409 4349
BmrI ACTGGGnnnn_n'
Cuts at: 162 570 905 1820 2093 3239 3970 4051 4386
Size: 408 335 915 273 1146 731 81 335
Cuts at: 4386 6530 162
Size: 2144 390
Fragments arranged by size:
2144 1146 915 731 408 390 335 335
273 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5125 5125
Size: 6758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 1273 1357 1669 2628 2768 3128 3371 171
Size: 1102 84 312 959 140 360 243 3558
Fragments arranged by size:
3558 1102 959 360 312 243 140 84
Bpu10I CC'TnA_GC
Cuts at: 434 1115 3628 434
Size: 681 2513 3564
Fragments arranged by size:
3564 2513 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 1420 3934 4813 5305 5603 361
Size: 1059 2514 879 492 298 1516
Fragments arranged by size:
2514 1516 1059 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1313 1429 2322 2501 2566 6462 1313
Size: 116 893 179 65 3896 1609
Fragments arranged by size:
3896 1609 893 179 116 65
BsaAI yAC'GTr
Cuts at: 798 1740 4111 798
Size: 942 2371 3445
Fragments arranged by size:
3445 2371 942
BsaBI GATnn'nnATC
Cuts at: 6 4272 6309 6
Size: 4266 2037 455
Fragments arranged by size:
4266 2037 455
BsaHI Gr'CG_yC
Cuts at: 1289 4189 4437 5139 6635 1289
Size: 2900 248 702 1496 1412
Fragments arranged by size:
2900 1496 1412 702 248
BsaWI w'CCGG_w
Cuts at: 716 839 2949 3947 4468 5146 5420 5567 5978
Size: 123 2110 998 521 678 274 147 411
Cuts at: 5978 716
Size: 1496
Fragments arranged by size:
2110 1496 998 678 521 411 274 147
123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 634 664 1792 1822 634
Size: 30 1128 30 5570
Fragments arranged by size:
5570 1128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1474 1489 1498 1666 2941 2983 2996 3067 3080
Size: 15 9 168 1275 42 13 71 13
Cuts at: 3080 3151 3161 3340 1474
Size: 71 10 179 4892
Fragments arranged by size:
4892 1275 179 168 71 71 42 15
13 13 10 9
BseYI C'CCAG_C
Cuts at: 758 1199 1619 1656 2352 2907 3033 4183 4941
Size: 441 420 37 696 555 126 1150 758
Cuts at: 4941 5518 6014 758
Size: 577 496 1502
Fragments arranged by size:
1502 1150 758 696 577 555 496 441
420 126 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1063 1768 2104 2321 2472 3238 1063
Size: 705 336 217 151 766 4583
Fragments arranged by size:
4583 766 705 336 217 151
BsiEI CG_ry'CG
Cuts at: 104 2184 4300 4346 5554 104
Size: 2080 2116 46 1208 1308
Fragments arranged by size:
2116 2080 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 872 1389 1514 1845 1889 3187 3772 4550 4740
Size: 517 125 331 44 1298 585 778 190
Cuts at: 4740 5532 872
Size: 792 2098
Fragments arranged by size:
2098 1298 792 778 585 517 331 190
125 44
Bsp1286I G_dGCh'C
Cuts at: 872 1353 1368 1389 1406 1505 1514 1845 1889
Size: 481 15 21 17 99 9 331 44
Cuts at: 1889 2197 2231 2556 3154 3187 3772 4383 4476
Size: 308 34 325 598 33 585 611 93
Cuts at: 4476 4550 4740 4802 5532 872
Size: 74 190 62 730 2098
Fragments arranged by size:
2098 730 611 598 585 481 331 325
308 190 99 93 74 62 44 34
33 21 17 15 9
BspEI T'CCGG_A
Cuts at: 716 839 2949 5978 716
Size: 123 2110 3029 1496
Fragments arranged by size:
3029 2110 1496 123
BspHI T'CATG_A
Cuts at: 2615 5934 2615
Size: 3319 3439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1220 2408 3483 3808 4022 4324 4705 1220
Size: 1188 1075 325 214 302 381 3273
Fragments arranged by size:
3273 1188 1075 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 233 438 2703 5050 5104 6588 6753 50
Size: 183 205 2265 2347 54 1484 165 55
Fragments arranged by size:
2347 2265 1484 205 183 165 55 54
BsrDI GCAATG_nn'
Cuts at: 299 399 1064 2282 4670 299
Size: 100 665 1218 2388 2387
Fragments arranged by size:
2388 2387 1218 665 100
BsrFI r'CCGG_y
Cuts at: 118 4756 4937 5146 118
Size: 4638 181 209 1730
Fragments arranged by size:
4638 1730 209 181
BssSI C'ACGA_G
Cuts at: 2714 5029 5387 2714
Size: 2315 358 4085
Fragments arranged by size:
4085 2315 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1086 1849 4135 1086
Size: 763 2286 3709
Fragments arranged by size:
3709 2286 763
BstBI TT'CG_AA
Cuts at: 199 5119 199
Size: 4920 1838
BstEII G'GTnAC_C
Cuts at: 2658 2658
Size: 6758
BstXI CCAn_nnnn'nTGG
Cuts at: 2068 3440 3505 3601 2068
Size: 1372 65 96 5225
Fragments arranged by size:
5225 1372 96 65
BstYI r'GATC_y
Cuts at: 1 330 713 2033 2346 3388 3783 4250 4273
Size: 329 383 1320 313 1042 395 467 23
Cuts at: 4273 4608 4854 5866 5952 5964 6310 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1320 1042 1012 467 449 395 383 346
335 329 313 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 179 669 682 1610 2375 179
Size: 490 13 928 765 4562
Fragments arranged by size:
4562 928 765 490 13
BtgI C'CryG_G
Cuts at: 873 1439 2061 2150 2477 2533 3101 3600 873
Size: 566 622 89 327 56 568 499 4031
Fragments arranged by size:
4031 622 568 566 499 327 89 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2251 2572 3693 4708 4889 5052 2251
Size: 321 1121 1015 181 163 3957
Fragments arranged by size:
3957 1121 1015 321 181 163
BtsI GCAGTG_nn'
Cuts at: 1581 3433 4001 1581
Size: 1852 568 4338
Fragments arranged by size:
4338 1852 568
DraI TTT'AAA
Cuts at: 3757 3757
Size: 6758
DraIII CAC_nnn'GTG
Cuts at: 1348 1348
Size: 6758
DrdI GACnn_nn'nnGTC
Cuts at: 190 913 4464 5322 190
Size: 723 3551 858 1626
Fragments arranged by size:
3551 1626 858 723
EaeI y'GGCC_r
Cuts at: 120 285 728 999 1175 1528 2181 2421 3582
Size: 165 443 271 176 353 653 240 1161
Cuts at: 3582 4343 4517 4908 4935 6280 120
Size: 761 174 391 27 1345 598
Fragments arranged by size:
1345 1161 761 653 598 443 391 353
271 240 176 174 165 27
EagI C'GGCC_G
Cuts at: 2181 4343 2181
Size: 2162 4596
EarI CTCTTCn'nnn_
Cuts at: 518 2768 4781 4991 518
Size: 2250 2013 210 2285
Fragments arranged by size:
2285 2250 2013 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4966 5276 5422 6568 4966
Size: 310 146 1146 5156
Fragments arranged by size:
5156 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3770 3770
Size: 6758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 368 988 1273 1304 1357 1358 1669 2628
Size: 197 620 285 31 53 1 311 959
Cuts at: 2628 2768 3128 3371 4583 5015 5762 6030 171
Size: 140 360 243 1212 432 747 268 899
Fragments arranged by size:
1212 959 899 747 620 432 360 311
285 268 243 197 140 53 31 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1876 2824 3479 1876
Size: 948 655 5155
Fragments arranged by size:
5155 948 655
EcoO109I rG'GnC_Cy
Cuts at: 430 474 846 906 1158 1492 2228 2552 2553
Size: 44 372 60 252 334 736 324 1
Cuts at: 2553 2638 3269 3299 3315 3396 3897 430
Size: 85 631 30 16 81 501 3291
Fragments arranged by size:
3291 736 631 501 372 334 324 252
85 81 60 44 30 16 1
EcoRI G'AATT_C
Cuts at: 394 3762 394
Size: 3368 3390
EcoRV GAT'ATC
Cuts at: 3239 3239
Size: 6758
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4777 4777
Size: 6758
FspI TGC'GCA
Cuts at: 4539 4539
Size: 6758
HaeII r_GCGC'y
Cuts at: 1674 1862 4192 4440 5462 6441 1674
Size: 188 2330 248 1022 979 1991
Fragments arranged by size:
2330 1991 1022 979 248 188
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1004 1036 1233 1265 2821 2853 3153 3185 3246
Size: 32 197 32 1556 32 300 32 61
Cuts at: 3246 3278 4600 4630 4632 4662 1004
Size: 32 1322 30 2 30 3100
Fragments arranged by size:
3100 1556 1322 300 197 61 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2211 3797 2211
Size: 1586 5172
KasI G'GCGC_C
Cuts at: 4188 4436 4188
Size: 248 6510
KpnI G_GTAC'C
Cuts at: 1618 3778 1618
Size: 2160 4598
MluI A'CGCG_T
Cuts at: 6191 6191
Size: 6758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 1823 3618 5429 5613 356
Size: 1467 1795 1811 184 1501
Fragments arranged by size:
1811 1795 1501 1467 184
MscI TGG'CCA
Cuts at: 730 1530 3584 4519 730
Size: 800 2054 935 2969
Fragments arranged by size:
2969 2054 935 800
MslI CAynn'nnrTG
Cuts at: 1024 2066 2126 4747 4874 1024
Size: 1042 60 2621 127 2908
Fragments arranged by size:
2908 2621 1042 127 60
MspA1I CmG'CkG
Cuts at: 1119 2323 2352 2809 3349 3621 3869 4183 4543
Size: 1204 29 457 540 272 248 314 360
Cuts at: 4543 5556 5801 1119
Size: 1013 245 2076
Fragments arranged by size:
2076 1204 1013 540 457 360 314 272
248 245 29
NaeI GCC'GGC
Cuts at: 4939 4939
Size: 6758
NarI GG'CG_CC
Cuts at: 4189 4437 4189
Size: 248 6510
NcoI C'CATG_G
Cuts at: 2150 2477 3101 3600 2150
Size: 327 624 499 5308
Fragments arranged by size:
5308 624 499 327
NgoMIV G'CCGG_C
Cuts at: 4937 4937
Size: 6758
NspI r_CATG'y
Cuts at: 789 2026 2085 3591 3811 4311 5218 789
Size: 1237 59 1506 220 500 907 2329
Fragments arranged by size:
2329 1506 1237 907 500 220 59
PasI CC'CwG_GG
Cuts at: 1496 1496
Size: 6758
PciI A'CATG_T
Cuts at: 2081 5214 2081
Size: 3133 3625
PflMI CCAn_nnn'nTGG
Cuts at: 294 3456 294
Size: 3162 3596
PfoI T'CCnGG_A
Cuts at: 422 467 744 422
Size: 45 277 6436
Fragments arranged by size:
6436 277 45
PmeI GTTT'AAAC
Cuts at: 3757 3757
Size: 6758
PmlI CAC'GTG
Cuts at: 798 798
Size: 6758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 666 5897 5929 634
Size: 32 5231 32 1463
Fragments arranged by size:
5231 1463 32 32
PpuMI rG'GwC_Cy
Cuts at: 846 906 1158 1492 2638 3269 846
Size: 60 252 334 1146 631 4335
Fragments arranged by size:
4335 1146 631 334 252 60
PspOMI G'GGCC_C
Cuts at: 2227 2552 2227
Size: 325 6433
PspXI vC'TCGA_Gb
Cuts at: 1102 3180 1102
Size: 2078 4680
PstI C_TGCA'G
Cuts at: 3480 3805 4490 3480
Size: 325 685 5748
Fragments arranged by size:
5748 685 325
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6758
PvuII CAG'CTG
Cuts at: 1119 2323 2809 3349 4183 4543 1119
Size: 1204 486 540 834 360 3334
Fragments arranged by size:
3334 1204 834 540 486 360
RsrII CG'GwC_CG
Cuts at: 4953 4953
Size: 6758
SacI G_AGCT'C
Cuts at: 3772 3772
Size: 6758
SalI G'TCGA_C
Cuts at: 3795 3795
Size: 6758
SanDI GG'GwC_CC
Cuts at: 1158 1158
Size: 6758
SapI GCTCTTCn'nnn_
Cuts at: 518 4781 4991 518
Size: 4263 210 2285
Fragments arranged by size:
4263 2285 210
SbfI CC_TGCA'GG
Cuts at: 3805 3805
Size: 6758
ScaI AGT'ACT
Cuts at: 6275 6275
Size: 6758
SexAI A'CCwGG_T
Cuts at: 1037 1344 1037
Size: 307 6451
SfcI C'TryA_G
Cuts at: 33 1537 3059 3476 3801 4486 5479 5670 33
Size: 1504 1522 417 325 685 993 191 1121
Fragments arranged by size:
1522 1504 1121 993 685 417 325 191
SfoI GGC'GCC
Cuts at: 4190 4438 4190
Size: 248 6510
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6758
SmaI CCC'GGG
Cuts at: 1777 3780 1777
Size: 2003 4755
SmlI C'TyrA_G
Cuts at: 340 1102 1399 3180 3913 4828 5320 5582 340
Size: 762 297 1781 733 915 492 262 1516
Fragments arranged by size:
1781 1516 915 762 733 492 297 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6758
SphI G_CATG'C
Cuts at: 2026 3811 4311 2026
Size: 1785 500 4473
Fragments arranged by size:
4473 1785 500
SspI AAT'ATT
Cuts at: 210 210
Size: 6758
StuI AGG'CCT
Cuts at: 741 973 3485 3670 741
Size: 232 2512 185 3829
Fragments arranged by size:
3829 2512 232 185
StyI C'CwwG_G
Cuts at: 1002 1008 1030 1163 2150 2477 2502 2689 3101
Size: 6 22 133 987 327 25 187 412
Cuts at: 3101 3272 3600 3892 4246 1002
Size: 171 328 292 354 3514
Fragments arranged by size:
3514 987 412 354 328 327 292 187
171 133 25 22 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2227 2410 3525 3761 4341 4474 4933 5142 2227
Size: 183 1115 236 580 133 459 209 3843
Fragments arranged by size:
3843 1115 580 459 236 209 183 133
TatI w'GTAC_w
Cuts at: 578 3247 6273 578
Size: 2669 3026 1063
Fragments arranged by size:
3026 2669 1063
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6275 6307 6275
Size: 32 6726
Tth111I GACn'n_nGTC
Cuts at: 3129 4555 3129
Size: 1426 5332
XbaI T'CTAG_A
Cuts at: 3789 3789
Size: 6758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1170 1523 2014 2065 2652 3607 3724 4253 1170
Size: 353 491 51 587 955 117 529 3675
Fragments arranged by size:
3675 955 587 529 491 353 117 51
XhoI C'TCGA_G
Cuts at: 1102 3180 1102
Size: 2078 4680
XmaI C'CCGG_G
Cuts at: 1775 3778 1775
Size: 2003 4755
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI BaeI
BfrBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
ClaI CspCI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PshAI PsiI PsrI
SacII SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI