Restriction map for
pF1KA0905
(Circular) (Six-base) MAPSORT of: pF1KA0905.seq Check: 8906 from: 1 to: 6773
pF1KA0905 6773 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 370 1909 370
Size: 1539 5234
AccI GT'mk_AC
Cuts at: 3811 3811
Size: 6773
Acc65I G'GTAC_C
Cuts at: 311 2709 3789 311
Size: 2398 1080 3295
Fragments arranged by size:
3295 2398 1080
AclI AA'CG_TT
Cuts at: 3552 6613 3552
Size: 3061 3712
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 1426 1733 2242 2984 3212 3488 3684 4598
Size: 150 307 509 742 228 276 196 914
Cuts at: 4598 5030 5777 6045 1276
Size: 432 747 268 2004
Fragments arranged by size:
2004 914 747 742 509 432 307 276
268 228 196 150
AfeI AGC'GCT
Cuts at: 3493 3493
Size: 6773
AflIII A'CryG_T
Cuts at: 4125 5229 6206 4125
Size: 1104 977 4692
Fragments arranged by size:
4692 1104 977
AgeI A'CCGG_T
Cuts at: 815 5161 815
Size: 4346 2427
AhdI GACnn_n'nnGTC
Cuts at: 3201 3201
Size: 6773
AleI CACnn'nnGTG
Cuts at: 1020 1999 1020
Size: 979 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 224 2193 2819 224
Size: 1969 626 4178
Fragments arranged by size:
4178 1969 626
AlwNI CAG_nnn'CTG
Cuts at: 925 2265 2560 4078 5645 925
Size: 1340 295 1518 1567 2053
Fragments arranged by size:
2053 1567 1518 1340 295
ApaLI G'TGCA_C
Cuts at: 5543 5543
Size: 6773
ApoI r'AATT_y
Cuts at: 1214 1510 1762 2098 2510 3777 6126 6382 1214
Size: 296 252 336 412 1267 2349 256 1605
Fragments arranged by size:
2349 1605 1267 412 336 296 256 252
AseI AT'TA_AT
Cuts at: 20 20
Size: 6773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6773
AvaI C'yCGr_G
Cuts at: 3793 3793
Size: 6773
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2693 2726 2693
Size: 33 6740
BamHI G'GATC_C
Cuts at: 3798 3798
Size: 6773
BanI G'GyrC_C
Cuts at: 311 2709 3364 3789 4203 4451 4486 311
Size: 2398 655 425 414 248 35 2598
Fragments arranged by size:
2598 2398 655 425 414 248 35
BanII G_rGCy'C
Cuts at: 154 1705 2988 3378 3787 4817 154
Size: 1551 1283 390 409 1030 2110
Fragments arranged by size:
2110 1551 1283 1030 409 390
BbeI G_GCGC'C
Cuts at: 4207 4455 4207
Size: 248 6525
BbsI GAAGACnn'nnnn_
Cuts at: 3248 3248
Size: 6773
BbvCI CC'TCA_GC
Cuts at: 1423 2792 1423
Size: 1369 5404
BciVI GTATCCnnnnn_n'
Cuts at: 1582 2765 4671 5432 6773 1582
Size: 1183 1906 761 1341 1582
Fragments arranged by size:
1906 1582 1341 1183 761
BclI T'GATC_A
Cuts at: 4293 4293
Size: 6773
BfrBI ATG'CAT
Cuts at: 750 2643 3672 750
Size: 1893 1029 3851
Fragments arranged by size:
3851 1893 1029
BglI GCCn_nnn'nGGC
Cuts at: 142 1654 3373 142
Size: 1512 1719 3542
Fragments arranged by size:
3542 1719 1512
BglII A'GATC_T
Cuts at: 1602 4288 6325 1602
Size: 2686 2037 2050
Fragments arranged by size:
2686 2050 2037
BlpI GC'TnA_GC
Cuts at: 197 1232 1361 3885 197
Size: 1035 129 2524 3085
Fragments arranged by size:
3085 2524 1035 129
Bme1580I G_kGCm'C
Cuts at: 3369 4398 4491 5547 3369
Size: 1029 93 1056 4595
Fragments arranged by size:
4595 1056 1029 93
BmrI ACTGGGnnnn_n'
Cuts at: 472 668 2876 3195 3985 4066 4401 6545 472
Size: 196 2208 319 790 81 335 2144 700
Fragments arranged by size:
2208 2144 790 700 335 319 196 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2170 5140 2170
Size: 2970 3803
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 452 1799 2672 2901 2975 3086 3218 386
Size: 66 1347 873 229 74 111 132 3941
Fragments arranged by size:
3941 1347 873 229 132 111 74 66
Bpu10I CC'TnA_GC
Cuts at: 1423 2384 2792 1423
Size: 961 408 5404
Fragments arranged by size:
5404 961 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 2150 2216 2294 3949 4828 5320 5618 256
Size: 1894 66 78 1655 879 492 298 1411
Fragments arranged by size:
1894 1655 1411 879 492 298 78 66
BsaI GGTCTCn'nnnn_
Cuts at: 3700 6477 3700
Size: 2777 3996
BsaAI yAC'GTr
Cuts at: 4126 4126
Size: 6773
BsaBI GATnn'nnATC
Cuts at: 6 4287 6324 6
Size: 4281 2037 455
Fragments arranged by size:
4281 2037 455
BsaHI Gr'CG_yC
Cuts at: 4204 4452 5154 6650 4204
Size: 248 702 1496 4327
Fragments arranged by size:
4327 1496 702 248
BsaWI w'CCGG_w
Cuts at: 815 3962 4483 5161 5435 5582 5993 815
Size: 3147 521 678 274 147 411 1595
Fragments arranged by size:
3147 1595 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1637 1667 2471 2501 2516 2546 3550 3580 1637
Size: 30 804 30 15 30 1004 30 4830
Fragments arranged by size:
4830 1004 804 30 30 30 30 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 288 1711 2798 2930 288
Size: 1423 1087 132 4131
Fragments arranged by size:
4131 1423 1087 132
BseYI C'CCAG_C
Cuts at: 2747 3759 4198 4956 5533 6029 2747
Size: 1012 439 758 577 496 3491
Fragments arranged by size:
3491 1012 758 577 496 439
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2210 3424 1474
Size: 736 1214 4823
Fragments arranged by size:
4823 1214 736
BsiEI CG_ry'CG
Cuts at: 104 4315 4361 5569 104
Size: 4211 46 1208 1308
Fragments arranged by size:
4211 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1736 2475 2988 3455 3787 4565 4755 5547 1736
Size: 739 513 467 332 778 190 792 2962
Fragments arranged by size:
2962 792 778 739 513 467 332 190
BsiWI C'GTAC_G
Cuts at: 2516 2516
Size: 6773
BsmI GAATG_Cn'
Cuts at: 231 752 1346 231
Size: 521 594 5658
Fragments arranged by size:
5658 594 521
Bsp1286I G_dGCh'C
Cuts at: 154 1705 1736 2475 2988 3369 3378 3455 3787
Size: 1551 31 739 513 381 9 77 332
Cuts at: 3787 4398 4491 4565 4755 4817 5547 154
Size: 611 93 74 190 62 730 1380
Fragments arranged by size:
1551 1380 739 730 611 513 381 332
190 93 77 74 62 31 9
BspEI T'CCGG_A
Cuts at: 5993 5993
Size: 6773
BspHI T'CATG_A
Cuts at: 3179 5949 3179
Size: 2770 4003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 370 1909 3823 4037 4339 4720 370
Size: 1539 1914 214 302 381 2423
Fragments arranged by size:
2423 1914 1539 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5065 5119 6603 6768 50
Size: 5015 54 1484 165 55
Fragments arranged by size:
5015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 626 2786 3623 4685 626
Size: 2160 837 1062 2714
Fragments arranged by size:
2714 2160 1062 837
BsrFI r'CCGG_y
Cuts at: 815 4771 4952 5161 815
Size: 3956 181 209 2427
Fragments arranged by size:
3956 2427 209 181
BssSI C'ACGA_G
Cuts at: 1562 5044 5402 1562
Size: 3482 358 2933
Fragments arranged by size:
3482 2933 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1452 3411 4150 1452
Size: 1959 739 4075
Fragments arranged by size:
4075 1959 739
BstBI TT'CG_AA
Cuts at: 1278 5134 1278
Size: 3856 2917
BstEII G'GTnAC_C
Cuts at: 818 3193 818
Size: 2375 4398
BstXI CCAn_nnnn'nTGG
Cuts at: 1908 2376 3759 1908
Size: 468 1383 4922
Fragments arranged by size:
4922 1383 468
BstYI r'GATC_y
Cuts at: 1 558 696 840 921 1602 2262 3480 3798
Size: 557 138 144 81 681 660 1218 318
Cuts at: 3798 4265 4288 4623 4869 5881 5967 5979 6325
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6325 1
Size: 449
Fragments arranged by size:
1218 1012 681 660 557 467 449 346
335 318 246 144 138 86 81 23
12
BtgI C'CryG_G
Cuts at: 2315 2607 3297 2315
Size: 292 690 5791
Fragments arranged by size:
5791 690 292
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4723 4904 5067 4723
Size: 181 163 6429
Fragments arranged by size:
6429 181 163
BtsI GCAGTG_nn'
Cuts at: 1994 2006 3010 3034 4016 1994
Size: 12 1004 24 982 4751
Fragments arranged by size:
4751 1004 982 24 12
DraI TTT'AAA
Cuts at: 3772 3772
Size: 6773
DraIII CAC_nnn'GTG
Cuts at: 868 1019 1386 1687 868
Size: 151 367 301 5954
Fragments arranged by size:
5954 367 301 151
DrdI GACnn_nn'nnGTC
Cuts at: 4479 5337 4479
Size: 858 5915
EaeI y'GGCC_r
Cuts at: 678 1892 1910 2562 3285 4358 4532 4923 4950
Size: 1214 18 652 723 1073 174 391 27
Cuts at: 4950 6295 678
Size: 1345 1156
Fragments arranged by size:
1345 1214 1156 1073 723 652 391 174
27 18
EagI C'GGCC_G
Cuts at: 4358 4358
Size: 6773
EarI CTCTTCn'nnn_
Cuts at: 306 1709 2910 4796 5006 306
Size: 1403 1201 1886 210 2073
Fragments arranged by size:
2073 1886 1403 1201 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2990 4981 5291 5437 6583 2990
Size: 1991 310 146 1146 3180
Fragments arranged by size:
3180 1991 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2986 3785 2986
Size: 799 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 452 1276 1426 1733 1799 2242 2672 2901
Size: 66 824 150 307 66 443 430 229
Cuts at: 2901 2975 2984 3086 3212 3218 3488 3684 4598
Size: 74 9 102 126 6 270 196 914
Cuts at: 4598 5030 5777 6045 386
Size: 432 747 268 1114
Fragments arranged by size:
1114 914 824 747 443 432 430 307
270 268 229 196 150 126 102 74
66 66 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2489 2489
Size: 6773
EcoO109I rG'GnC_Cy
Cuts at: 331 3076 3912 331
Size: 2745 836 3192
Fragments arranged by size:
3192 2745 836
EcoRI G'AATT_C
Cuts at: 3777 3777
Size: 6773
EcoRV GAT'ATC
Cuts at: 618 618
Size: 6773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1563 2264 3272 4792 1563
Size: 701 1008 1520 3544
Fragments arranged by size:
3544 1520 1008 701
FspI TGC'GCA
Cuts at: 4554 4554
Size: 6773
HaeII r_GCGC'y
Cuts at: 3495 4207 4455 5477 6456 3495
Size: 712 248 1022 979 3812
Fragments arranged by size:
3812 1022 979 712 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 265 297 825 857 1916 1948 2214 2246 3187
Size: 32 528 32 1059 32 266 32 941
Cuts at: 3187 3219 4615 4645 4647 4677 265
Size: 32 1396 30 2 30 2361
Fragments arranged by size:
2361 1396 1059 941 528 266 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1146 1866 3812 1146
Size: 720 1946 4107
Fragments arranged by size:
4107 1946 720
HindIII A'AGCT_T
Cuts at: 1150 1150
Size: 6773
KasI G'GCGC_C
Cuts at: 4203 4451 4203
Size: 248 6525
KpnI G_GTAC'C
Cuts at: 315 2713 3793 315
Size: 2398 1080 3295
Fragments arranged by size:
3295 2398 1080
MfeI C'AATT_G
Cuts at: 202 902 202
Size: 700 6073
MluI A'CGCG_T
Cuts at: 6206 6206
Size: 6773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2200 3070 5444 5628 2200
Size: 870 2374 184 3345
Fragments arranged by size:
3345 2374 870 184
MscI TGG'CCA
Cuts at: 1912 2564 3287 4534 1912
Size: 652 723 1247 4151
Fragments arranged by size:
4151 1247 723 652
MslI CAynn'nnrTG
Cuts at: 776 1020 1999 2648 4762 4889 776
Size: 244 979 649 2114 127 2660
Fragments arranged by size:
2660 2114 979 649 244 127
MspA1I CmG'CkG
Cuts at: 623 1069 2880 3884 4198 4558 5571 5816 623
Size: 446 1811 1004 314 360 1013 245 1580
Fragments arranged by size:
1811 1580 1013 1004 446 360 314 245
NaeI GCC'GGC
Cuts at: 4954 4954
Size: 6773
NarI GG'CG_CC
Cuts at: 4204 4452 4204
Size: 248 6525
NcoI C'CATG_G
Cuts at: 2315 2607 3297 2315
Size: 292 690 5791
Fragments arranged by size:
5791 690 292
NdeI CA'TA_TG
Cuts at: 553 2696 553
Size: 2143 4630
NgoMIV G'CCGG_C
Cuts at: 4952 4952
Size: 6773
NsiI A_TGCA'T
Cuts at: 752 2645 3674 752
Size: 1893 1029 3851
Fragments arranged by size:
3851 1893 1029
NspI r_CATG'y
Cuts at: 2174 2219 3333 3403 3826 4326 5233 2174
Size: 45 1114 70 423 500 907 3714
Fragments arranged by size:
3714 1114 907 500 423 70 45
PasI CC'CwG_GG
Cuts at: 3370 3370
Size: 6773
PciI A'CATG_T
Cuts at: 5229 5229
Size: 6773
PflMI CCAn_nnn'nTGG
Cuts at: 692 2560 692
Size: 1868 4905
PfoI T'CCnGG_A
Cuts at: 872 3018 872
Size: 2146 4627
PmeI GTTT'AAAC
Cuts at: 3772 3772
Size: 6773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 180 212 3063 3095 5912 5944 180
Size: 32 2851 32 2817 32 1009
Fragments arranged by size:
2851 2817 1009 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3076 3076
Size: 6773
PsiI TTA'TAA
Cuts at: 338 338
Size: 6773
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3015 3047 3015
Size: 32 6741
PstI C_TGCA'G
Cuts at: 2001 2073 2162 2403 3218 3820 4505 2001
Size: 72 89 241 815 602 685 4269
Fragments arranged by size:
4269 815 685 602 241 89 72
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6773
PvuII CAG'CTG
Cuts at: 623 1069 2880 4198 4558 623
Size: 446 1811 1318 360 2838
Fragments arranged by size:
2838 1811 1318 446 360
RsrII CG'GwC_CG
Cuts at: 4968 4968
Size: 6773
SacI G_AGCT'C
Cuts at: 2988 3787 2988
Size: 799 5974
SalI G'TCGA_C
Cuts at: 3810 3810
Size: 6773
SapI GCTCTTCn'nnn_
Cuts at: 1709 4796 5006 1709
Size: 3087 210 3476
Fragments arranged by size:
3476 3087 210
SbfI CC_TGCA'GG
Cuts at: 3820 3820
Size: 6773
ScaI AGT'ACT
Cuts at: 2328 6290 2328
Size: 3962 2811
SfcI C'TryA_G
Cuts at: 33 1997 2069 2158 2330 2399 2486 3214 3816
Size: 1964 72 89 172 69 87 728 602
Cuts at: 3816 4501 5494 5685 33
Size: 685 993 191 1121
Fragments arranged by size:
1964 1121 993 728 685 602 191 172
89 87 72 69
SfoI GGC'GCC
Cuts at: 4205 4453 4205
Size: 248 6525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6773
SmaI CCC'GGG
Cuts at: 3795 3795
Size: 6773
SmlI C'TyrA_G
Cuts at: 235 2165 2231 2309 3928 4843 5335 5597 235
Size: 1930 66 78 1619 915 492 262 1411
Fragments arranged by size:
1930 1619 1411 915 492 262 78 66
SpeI A'CTAG_T
Cuts at: 67 2323 67
Size: 2256 4517
SphI G_CATG'C
Cuts at: 3333 3826 4326 3333
Size: 493 500 5780
Fragments arranged by size:
5780 500 493
SspI AAT'ATT
Cuts at: 399 2599 3357 399
Size: 2200 758 3815
Fragments arranged by size:
3815 2200 758
StuI AGG'CCT
Cuts at: 1284 2547 2902 1284
Size: 1263 355 5155
Fragments arranged by size:
5155 1263 355
StyI C'CwwG_G
Cuts at: 269 488 2315 2405 2537 2607 3297 3907 4261
Size: 219 1827 90 132 70 690 610 354
Cuts at: 4261 269
Size: 2781
Fragments arranged by size:
2781 1827 690 610 354 219 132 90
70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2719 3181 3751 3760 4356 4489 4948 5157 2719
Size: 462 570 9 596 133 459 209 4335
Fragments arranged by size:
4335 596 570 462 459 209 133 9
TatI w'GTAC_w
Cuts at: 2326 6288 2326
Size: 3962 2811
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 1637 1669 6290 6322 854
Size: 32 751 32 4621 32 1305
Fragments arranged by size:
4621 1305 751 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4570 4570
Size: 6773
XbaI T'CTAG_A
Cuts at: 3804 3804
Size: 6773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 894 3295 3552 4268 894
Size: 2401 257 716 3399
Fragments arranged by size:
3399 2401 716 257
XmaI C'CCGG_G
Cuts at: 3793 3793
Size: 6773
XmnI GAAnn'nnTTC
Cuts at: 502 1344 502
Size: 842 5931
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AloI ApaI AscI AvrII BcgI BmgBI
BmtI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
FseI FspAI HpaI NheI NotI NruI PacI PmlI
PshAI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI