Restriction map for
pF1KA0913
(Circular) (Six-base) MAPSORT of: pF1KA0913.seq Check: 6356 from: 1 to: 8501
pF1KA0913 8501 bp
With 182 enzymes: SgfI *
August 18, 2008 14:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 345 1894 4282 4342 4559 4982 5246 345
Size: 1549 2388 60 217 423 264 3600
Fragments arranged by size:
3600 2388 1549 423 264 217 60
AccI GT'mk_AC
Cuts at: 5539 5539
Size: 8501
Acc65I G'GTAC_C
Cuts at: 301 5517 301
Size: 5216 3285
AclI AA'CG_TT
Cuts at: 8341 8341
Size: 8501
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1486 1832 2311 4259 6326 6758 7111 7773
Size: 457 346 479 1948 2067 432 353 662
Cuts at: 7773 1029
Size: 1757
Fragments arranged by size:
2067 1948 1757 662 479 457 432 353
346
AfeI AGC'GCT
Cuts at: 145 5272 145
Size: 5127 3374
AflII C'TTAA_G
Cuts at: 3019 3483 3019
Size: 464 8037
AflIII A'CryG_T
Cuts at: 2953 5853 7653 7934 2953
Size: 2900 1800 281 3520
Fragments arranged by size:
3520 2900 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 4940 4940
Size: 8501
AleI CACnn'nnGTG
Cuts at: 575 1295 4450 4729 4810 5240 575
Size: 720 3155 279 81 430 3836
Fragments arranged by size:
3836 3155 720 430 279 81
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 827 827
Size: 8501
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 2455
Size: 32 8469
AlwNI CAG_nnn'CTG
Cuts at: 409 1093 2062 2167 2406 2893 3078 3187 3196
Size: 684 969 105 239 487 185 109 9
Cuts at: 3196 3223 4402 5211 5806 7244 409
Size: 27 1179 809 595 1438 1666
Fragments arranged by size:
1666 1438 1179 969 809 684 595 487
239 185 109 105 27 9
ApaI G_GGCC'C
Cuts at: 260 944 1635 1641 3148 3165 3516 4149 4396
Size: 684 691 6 1507 17 351 633 247
Cuts at: 4396 260
Size: 4365
Fragments arranged by size:
4365 1507 691 684 633 351 247 17
6
ApaLI G'TGCA_C
Cuts at: 5128 7339 5128
Size: 2211 6290
ApoI r'AATT_y
Cuts at: 5505 7854 8110 5505
Size: 2349 256 5896
Fragments arranged by size:
5896 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8501
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8501
AvaI C'yCGr_G
Cuts at: 1634 1688 3629 3673 4485 4924 5473 5521 1634
Size: 54 1941 44 812 439 549 48 4614
Fragments arranged by size:
4614 1941 812 549 439 54 48 44
AvrII C'CTAG_G
Cuts at: 4677 4677
Size: 8501
BamHI G'GATC_C
Cuts at: 5526 5526
Size: 8501
BanI G'GyrC_C
Cuts at: 301 588 1427 1657 2717 4004 4452 4755 4861
Size: 287 839 230 1060 1287 448 303 106
Cuts at: 4861 5517 5931 6179 6214 301
Size: 656 414 248 35 2588
Fragments arranged by size:
2588 1287 1060 839 656 448 414 303
287 248 230 106 35
BanII G_rGCy'C
Cuts at: 260 762 836 924 944 1635 1641 1900 2038
Size: 502 74 88 20 691 6 259 138
Cuts at: 2038 3148 3165 3516 3583 3630 4149 4396 4686
Size: 1110 17 351 67 47 519 247 290
Cuts at: 4686 5170 5515 6545 260
Size: 484 345 1030 2216
Fragments arranged by size:
2216 1110 1030 691 519 502 484 351
345 290 259 247 138 88 74 67
47 20 17 6
BbeI G_GCGC'C
Cuts at: 1431 5935 6183 1431
Size: 4504 248 3749
Fragments arranged by size:
4504 3749 248
BbsI GAAGACnn'nnnn_
Cuts at: 878 1035 1064 1510 1943 2914 3289 3643 3751
Size: 157 29 446 433 971 375 354 108
Cuts at: 3751 4620 878
Size: 869 4759
Fragments arranged by size:
4759 971 869 446 433 375 354 157
108 29
BbvCI CC'TCA_GC
Cuts at: 839 945 1281 2339 839
Size: 106 336 1058 7001
Fragments arranged by size:
7001 1058 336 106
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 687 721 2263 2297 3664 3698 3732 3766 687
Size: 34 1542 34 1367 34 34 34 5422
Fragments arranged by size:
5422 1542 1367 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2481 6399 7455 8501 2481
Size: 3918 1056 1046 2481
Fragments arranged by size:
3918 2481 1056 1046
BclI T'GATC_A
Cuts at: 2990 3755 6021 2990
Size: 765 2266 5470
Fragments arranged by size:
5470 2266 765
BfrBI ATG'CAT
Cuts at: 4120 4120
Size: 8501
BglI GCCn_nnn'nGGC
Cuts at: 1650 2075 3160 4198 1650
Size: 425 1085 1038 5953
Fragments arranged by size:
5953 1085 1038 425
BglII A'GATC_T
Cuts at: 3026 6016 8053 3026
Size: 2990 2037 3474
Fragments arranged by size:
3474 2990 2037
BlpI GC'TnA_GC
Cuts at: 860 1159 1899 1927 5164 5613 860
Size: 299 740 28 3237 449 3748
Fragments arranged by size:
3748 3237 740 449 299 28
Bme1580I G_kGCm'C
Cuts at: 260 944 1310 1635 1641 3148 3165 3453 3516
Size: 684 366 325 6 1507 17 288 63
Cuts at: 3516 4007 4149 4396 4457 4640 5031 5132 5247
Size: 491 142 247 61 183 391 101 115
Cuts at: 5247 5367 6126 6219 7343 260
Size: 120 759 93 1124 1418
Fragments arranged by size:
1507 1418 1124 759 684 491 391 366
325 288 247 183 142 120 115 101
93 63 61 17 6
BmgBI CAC'GTC
Cuts at: 705 2956 705
Size: 2251 6250
BmrI ACTGGGnnnn_n'
Cuts at: 757 885 1572 1879 4507 5713 5794 6129 8273
Size: 128 687 307 2628 1206 81 335 2144
Cuts at: 8273 757
Size: 985
Fragments arranged by size:
2628 2144 1206 985 687 335 307 128
81
BmtI G_CTAG'C
Cuts at: 2413 2413
Size: 8501
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 165 5237 6868 165
Size: 5072 1631 1798
Fragments arranged by size:
5072 1798 1631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 1699 1969 2594 2798 3576 5029 1531
Size: 168 270 625 204 778 1453 5003
Fragments arranged by size:
5003 1453 778 625 270 204 168
Bpu10I CC'TnA_GC
Cuts at: 410 839 945 1281 2339 2509 4096 4217 410
Size: 429 106 336 1058 170 1587 121 4694
Fragments arranged by size:
4694 1587 1058 429 336 170 121 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2413 2659 4839 5413 5677 6556 7270 7568 2413
Size: 246 2180 574 264 879 714 298 3346
Fragments arranged by size:
3346 2180 879 714 574 298 264 246
BsaI GGTCTCn'nnnn_
Cuts at: 3131 3486 5472 8205 3131
Size: 355 1986 2733 3427
Fragments arranged by size:
3427 2733 1986 355
BsaAI yAC'GTr
Cuts at: 1276 3913 5127 5854 1276
Size: 2637 1214 727 3923
Fragments arranged by size:
3923 2637 1214 727
BsaBI GATnn'nnATC
Cuts at: 6 6015 8052 6
Size: 6009 2037 455
Fragments arranged by size:
6009 2037 455
BsaHI Gr'CG_yC
Cuts at: 1145 1428 5386 5932 6180 6882 8378 1145
Size: 283 3958 546 248 702 1496 1268
Fragments arranged by size:
3958 1496 1268 702 546 283 248
BsaWI w'CCGG_w
Cuts at: 924 5357 5690 6211 6889 7300 7447 7721 924
Size: 4433 333 521 678 411 147 274 1704
Fragments arranged by size:
4433 1704 678 521 411 333 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 103 133 2311 2341 5038 5068 103
Size: 30 2178 30 2697 30 3536
Fragments arranged by size:
3536 2697 2178 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 190 2194 2281 2881 3573 3638 4697 4942 4945
Size: 2004 87 600 692 65 1059 245 3
Cuts at: 4945 190
Size: 3746
Fragments arranged by size:
3746 2004 1059 692 600 245 87 65
3
BseYI C'CCAG_C
Cuts at: 682 1421 1628 2227 2260 2284 3223 3509 3995
Size: 739 207 599 33 24 939 286 486
Cuts at: 3995 4363 4581 4639 4891 5016 5094 5112 5926
Size: 368 218 58 252 125 78 18 814
Cuts at: 5926 6684 7349 7757 682
Size: 758 665 408 1426
Fragments arranged by size:
1426 939 814 758 739 665 599 486
408 368 286 252 218 207 125 78
58 33 24 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 681 997 1394 3704 3823 3865 4174 4507 4951
Size: 316 397 2310 119 42 309 333 444
Cuts at: 4951 5287 681
Size: 336 3895
Fragments arranged by size:
3895 2310 444 397 336 333 316 309
119 42
BsiEI CG_ry'CG
Cuts at: 104 6043 6089 7319 104
Size: 5939 46 1230 1286
Fragments arranged by size:
5939 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 836 924 2273 2558 2814 5132 5515 6293 6483
Size: 88 1349 285 256 2318 383 778 190
Cuts at: 6483 7343 836
Size: 860 1994
Fragments arranged by size:
2318 1994 1349 860 778 383 285 256
190 88
BsiWI C'GTAC_G
Cuts at: 1272 1272
Size: 8501
BsmI GAATG_Cn'
Cuts at: 314 2217 3997 4672 5003 5355 314
Size: 1903 1780 675 331 352 3460
Fragments arranged by size:
3460 1903 1780 675 352 331
BsmBI CGTCTCn'nnnn_
Cuts at: 871 2386 5191 871
Size: 1515 2805 4181
Fragments arranged by size:
4181 2805 1515
Bsp1286I G_dGCh'C
Cuts at: 260 762 836 924 944 1310 1635 1641 1900
Size: 502 74 88 20 366 325 6 259
Cuts at: 1900 2038 2273 2558 2814 3148 3165 3453 3516
Size: 138 235 285 256 334 17 288 63
Cuts at: 3516 3583 3630 4007 4149 4396 4457 4640 4686
Size: 67 47 377 142 247 61 183 46
Cuts at: 4686 5031 5132 5170 5247 5367 5515 6126 6219
Size: 345 101 38 77 120 148 611 93
Cuts at: 6219 6293 6483 6545 7343 260
Size: 74 190 62 798 1418
Fragments arranged by size:
1418 798 611 502 377 366 345 334
325 288 285 259 256 247 235 190
183 148 142 138 120 101 93 88
77 74 74 67 63 62 61 47
46 38 20 17 6
BspEI T'CCGG_A
Cuts at: 924 5357 924
Size: 4433 4068
BspHI T'CATG_A
Cuts at: 6933 6933
Size: 8501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 345 1163 1894 3644 3845 4282 4342 4559 4982
Size: 818 731 1750 201 437 60 217 423
Cuts at: 4982 5246 5551 5765 6067 6448 345
Size: 264 305 214 302 381 2398
Fragments arranged by size:
2398 1750 818 731 437 423 381 305
302 264 217 214 201 60
BsrBI CCG'CTC
Cuts at: 50 1515 1667 4907 5060 5221 6793 6847 8331
Size: 1465 152 3240 153 161 1572 54 1484
Cuts at: 8331 8496 50
Size: 165 55
Fragments arranged by size:
3240 1572 1484 1465 165 161 153 152
55 54
BsrDI GCAATG_nn'
Cuts at: 553 1203 2142 6413 553
Size: 650 939 4271 2641
Fragments arranged by size:
4271 2641 939 650
BsrFI r'CCGG_y
Cuts at: 1694 4407 6499 6680 1694
Size: 2713 2092 181 3515
Fragments arranged by size:
3515 2713 2092 181
BssSI C'ACGA_G
Cuts at: 2475 6772 7480 2475
Size: 4297 708 3496
Fragments arranged by size:
4297 3496 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1348 2167 2890 2893 3196 4974 5878 1348
Size: 819 723 3 303 1778 904 3971
Fragments arranged by size:
3971 1778 904 819 723 303 3
BstBI TT'CG_AA
Cuts at: 6862 6862
Size: 8501
BstEII G'GTnAC_C
Cuts at: 2968 2968
Size: 8501
BstXI CCAn_nnnn'nTGG
Cuts at: 1869 2628 4516 4812 4899 1869
Size: 759 1888 296 87 5471
Fragments arranged by size:
5471 1888 759 296 87
BstYI r'GATC_y
Cuts at: 1 845 2403 2681 3026 4136 5526 5993 6016
Size: 844 1558 278 345 1110 1390 467 23
Cuts at: 6016 6351 6597 6903 6915 7001 8053 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1558 1390 1110 1052 844 467 449 345
335 306 278 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 3180 3807 4019 4379 3180
Size: 627 212 360 7302
Fragments arranged by size:
7302 627 360 212
BtgI C'CryG_G
Cuts at: 107 1076 3953 4751 5390 107
Size: 969 2877 798 639 3218
Fragments arranged by size:
3218 2877 969 798 639
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6451 6632 6795 6451
Size: 181 163 8157
Fragments arranged by size:
8157 181 163
BtsI GCAGTG_nn'
Cuts at: 300 501 1009 1302 1462 1890 2373 3453 4162
Size: 201 508 293 160 428 483 1080 709
Cuts at: 4162 4208 4436 4532 4724 4798 4830 4867 5744
Size: 46 228 96 192 74 32 37 877
Cuts at: 5744 300
Size: 3057
Fragments arranged by size:
3057 1080 877 709 508 483 428 293
228 201 192 160 96 74 46 37
32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 338 373 1005 1040 338
Size: 35 632 35 7799
Fragments arranged by size:
7799 632 35 35
DraI TTT'AAA
Cuts at: 5500 5500
Size: 8501
DraIII CAC_nnn'GTG
Cuts at: 708 1385 1873 2752 4660 708
Size: 677 488 879 1908 4549
Fragments arranged by size:
4549 1908 879 677 488
DrdI GACnn_nn'nnGTC
Cuts at: 1375 6207 7551 1375
Size: 4832 1344 2325
Fragments arranged by size:
4832 2325 1344
EaeI y'GGCC_r
Cuts at: 485 1394 1534 1651 1961 2783 3431 4506 5480
Size: 909 140 117 310 822 648 1075 974
Cuts at: 5480 6086 6260 6651 6678 8023 485
Size: 606 174 391 27 1345 963
Fragments arranged by size:
1345 1075 974 963 909 822 648 606
391 310 174 140 117 27
EagI C'GGCC_G
Cuts at: 6086 6086
Size: 8501
EarI CTCTTCn'nnn_
Cuts at: 452 532 727 1525 1562 2234 3567 4202 6524
Size: 80 195 798 37 672 1333 635 2322
Cuts at: 6524 6734 452
Size: 210 2219
Fragments arranged by size:
2322 2219 1333 798 672 635 210 195
80 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 299 1925 6709 7451 7597 8311 299
Size: 1626 4784 742 146 714 489
Fragments arranged by size:
4784 1626 742 714 489 146
EcoICRI GAG'CTC
Cuts at: 834 922 5513 834
Size: 88 4591 3822
Fragments arranged by size:
4591 3822 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1029 1486 1531 1699 1832 1969 2311 2594 2798
Size: 457 45 168 133 137 342 283 204
Cuts at: 2798 3576 4259 5029 6326 6758 7111 7773 1029
Size: 778 683 770 1297 432 353 662 1757
Fragments arranged by size:
1757 1297 778 770 683 662 457 432
353 342 283 204 168 137 133 45
EcoNI CCTnn'n_nnAGG
Cuts at: 611 2512 611
Size: 1901 6600
EcoO109I rG'GnC_Cy
Cuts at: 256 549 617 940 941 1638 1674 1773 1792
Size: 293 68 323 1 697 36 99 19
Cuts at: 1792 2343 3144 3161 3162 3193 3232 3395 3513
Size: 551 801 17 1 31 39 163 118
Cuts at: 3513 3823 4146 4392 5640 256
Size: 310 323 246 1248 3117
Fragments arranged by size:
3117 1248 801 697 551 323 323 310
293 246 163 118 99 68 39 36
31 19 17 1 1
EcoRI G'AATT_C
Cuts at: 5505 5505
Size: 8501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2538 3045 6520 2538
Size: 507 3475 4519
Fragments arranged by size:
4519 3475 507
FspI TGC'GCA
Cuts at: 2354 6282 2354
Size: 3928 4573
FspAI rTGC'GCAy
Cuts at: 2354 2354
Size: 8501
HaeII r_GCGC'y
Cuts at: 147 1431 5274 5375 5935 6183 7413 8184 147
Size: 1284 3843 101 560 248 1230 771 464
Fragments arranged by size:
3843 1284 1230 771 560 464 248 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 311 343 591 623 749 763 781 795 1042
Size: 32 248 32 126 14 18 14 247
Cuts at: 1042 1074 4012 4030 4044 4062 4926 4958 5004
Size: 32 2938 18 14 18 864 32 46
Cuts at: 5004 5036 6343 6373 6375 6405 311
Size: 32 1307 30 2 30 2407
Fragments arranged by size:
2938 2407 1307 864 248 247 126 46
32 32 32 32 32 30 30 18
18 18 14 14 14 2
HincII GTy'rAC
Cuts at: 896 2531 5540 896
Size: 1635 3009 3857
Fragments arranged by size:
3857 3009 1635
HindIII A'AGCT_T
Cuts at: 3016 3016
Size: 8501
KasI G'GCGC_C
Cuts at: 1427 5931 6179 1427
Size: 4504 248 3749
Fragments arranged by size:
4504 3749 248
KpnI G_GTAC'C
Cuts at: 305 5521 305
Size: 5216 3285
MluI A'CGCG_T
Cuts at: 7934 7934
Size: 8501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1193 2154 4163 4974 7260 7444 1193
Size: 961 2009 811 2286 184 2250
Fragments arranged by size:
2286 2250 2009 961 811 184
MscI TGG'CCA
Cuts at: 487 2785 3433 4508 5482 6262 487
Size: 2298 648 1075 974 780 2726
Fragments arranged by size:
2726 2298 1075 974 780 648
MslI CAynn'nnrTG
Cuts at: 575 1295 1352 2354 3427 4115 4201 4450 4465
Size: 720 57 1002 1073 688 86 249 15
Cuts at: 4465 4729 4810 4976 5240 5395 6490 6617 575
Size: 264 81 166 264 155 1095 127 2459
Fragments arranged by size:
2459 1095 1073 1002 720 688 264 264
249 166 155 127 86 81 57 15
MspA1I CmG'CkG
Cuts at: 253 647 668 801 1093 1425 1449 1540 1601
Size: 394 21 133 292 332 24 91 61
Cuts at: 1601 1723 1788 1810 3718 4143 4423 5208 5463
Size: 122 65 22 1908 425 280 785 255
Cuts at: 5463 5612 5926 6286 7070 7315 253
Size: 149 314 360 784 245 1439
Fragments arranged by size:
1908 1439 785 784 425 394 360 332
314 292 280 255 245 149 133 122
91 65 61 24 22 21
NaeI GCC'GGC
Cuts at: 6682 6682
Size: 8501
NarI GG'CG_CC
Cuts at: 1428 5932 6180 1428
Size: 4504 248 3749
Fragments arranged by size:
4504 3749 248
NcoI C'CATG_G
Cuts at: 107 3953 4751 5390 107
Size: 3846 798 639 3218
Fragments arranged by size:
3846 3218 798 639
NgoMIV G'CCGG_C
Cuts at: 6680 6680
Size: 8501
NheI G'CTAG_C
Cuts at: 2409 2409
Size: 8501
NsiI A_TGCA'T
Cuts at: 4122 4122
Size: 8501
NspI r_CATG'y
Cuts at: 537 1109 1365 2353 2586 4614 4981 5292 5554
Size: 572 256 988 233 2028 367 311 262
Cuts at: 5554 6054 7657 537
Size: 500 1603 1381
Fragments arranged by size:
2028 1603 1381 988 572 500 367 311
262 256 233
PasI CC'CwG_GG
Cuts at: 1995 3157 3576 4396 1995
Size: 1162 419 820 6100
Fragments arranged by size:
6100 1162 820 419
PciI A'CATG_T
Cuts at: 7653 7653
Size: 8501
PflMI CCAn_nnn'nTGG
Cuts at: 708 1585 2565 3524 3954 5262 708
Size: 877 980 959 430 1308 3947
Fragments arranged by size:
3947 1308 980 959 877 430
PfoI T'CCnGG_A
Cuts at: 869 3934 4491 869
Size: 3065 557 4879
Fragments arranged by size:
4879 3065 557
PmeI GTTT'AAAC
Cuts at: 5500 5500
Size: 8501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2921 2953 6947 6979 2921
Size: 32 3994 32 4443
Fragments arranged by size:
4443 3994 32 32
PpuMI rG'GwC_Cy
Cuts at: 549 1773 1792 3395 3823 549
Size: 1224 19 1603 428 5227
Fragments arranged by size:
5227 1603 1224 428 19
PspOMI G'GGCC_C
Cuts at: 256 940 1631 1637 3144 3161 3512 4145 4392
Size: 684 691 6 1507 17 351 633 247
Cuts at: 4392 256
Size: 4365
Fragments arranged by size:
4365 1507 691 684 633 351 247 17
6
PstI C_TGCA'G
Cuts at: 667 747 2172 3192 4293 5183 5207 5548 6233
Size: 80 1425 1020 1101 890 24 341 685
Cuts at: 6233 667
Size: 2935
Fragments arranged by size:
2935 1425 1101 1020 890 685 341 80
24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8501
PvuII CAG'CTG
Cuts at: 668 801 1449 1723 1810 3718 5926 6286 668
Size: 133 648 274 87 1908 2208 360 2883
Fragments arranged by size:
2883 2208 1908 648 360 274 133 87
RsrII CG'GwC_CG
Cuts at: 165 6696 165
Size: 6531 1970
SacI G_AGCT'C
Cuts at: 836 924 5515 836
Size: 88 4591 3822
Fragments arranged by size:
4591 3822 88
SalI G'TCGA_C
Cuts at: 5538 5538
Size: 8501
SanDI GG'GwC_CC
Cuts at: 1773 1792 3395 1773
Size: 19 1603 6879
Fragments arranged by size:
6879 1603 19
SapI GCTCTTCn'nnn_
Cuts at: 532 1525 3567 6524 6734 532
Size: 993 2042 2957 210 2299
Fragments arranged by size:
2957 2299 2042 993 210
SbfI CC_TGCA'GG
Cuts at: 5548 5548
Size: 8501
ScaI AGT'ACT
Cuts at: 963 8018 963
Size: 7055 1446
SfcI C'TryA_G
Cuts at: 33 418 581 663 743 787 1596 2120 2168
Size: 385 163 82 80 44 809 524 48
Cuts at: 2168 2361 2599 3188 4289 4329 4540 4557 5179
Size: 193 238 589 1101 40 211 17 622
Cuts at: 5179 5203 5419 5544 6229 7197 7388 33
Size: 24 216 125 685 968 191 1146
Fragments arranged by size:
1146 1101 968 809 685 622 589 524
385 238 216 211 193 191 163 125
82 80 48 44 40 24 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 2075 2075
Size: 8501
SfoI GGC'GCC
Cuts at: 1429 5933 6181 1429
Size: 4504 248 3749
Fragments arranged by size:
4504 3749 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8501
SmaI CCC'GGG
Cuts at: 1636 1690 3631 4487 5523 1636
Size: 54 1941 856 1036 4614
Fragments arranged by size:
4614 1941 1036 856 54
SmlI C'TyrA_G
Cuts at: 2428 2674 3019 3483 4818 5428 5473 5656 6571
Size: 246 345 464 1335 610 45 183 915
Cuts at: 6571 7285 7547 2428
Size: 714 262 3382
Fragments arranged by size:
3382 1335 915 714 610 464 345 262
246 183 45
SnaBI TAC'GTA
Cuts at: 1276 1276
Size: 8501
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8501
SphI G_CATG'C
Cuts at: 537 2353 4981 5554 6054 537
Size: 1816 2628 573 500 2984
Fragments arranged by size:
2984 2628 1816 573 500
SrfI GCCC'GGGC
Cuts at: 1636 3631 1636
Size: 1995 6506
StuI AGG'CCT
Cuts at: 1549 1573 2374 2607 2626 4192 1549
Size: 24 801 233 19 1566 5858
Fragments arranged by size:
5858 1566 801 233 24 19
StyI C'CwwG_G
Cuts at: 107 1733 1862 2450 2591 2621 2627 2897 3322
Size: 1626 129 588 141 30 6 270 425
Cuts at: 3322 3873 3953 4677 4751 4857 5153 5390 5447
Size: 551 80 724 74 106 296 237 57
Cuts at: 5447 5635 5989 107
Size: 188 354 2619
Fragments arranged by size:
2619 1626 724 588 551 425 354 296
270 237 188 141 129 106 80 74
57 30 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 715 1753 1861 2481 3062 4595 4773 6084 6217
Size: 1038 108 620 581 1533 178 1311 133
Cuts at: 6217 6676 6885 715
Size: 459 209 2331
Fragments arranged by size:
2331 1533 1311 1038 620 581 459 209
178 133 108
TatI w'GTAC_w
Cuts at: 961 4767 5294 8016 961
Size: 3806 527 2722 1446
Fragments arranged by size:
3806 2722 1446 527
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2736 2768 4755 4787 8018 8050 2736
Size: 32 1987 32 3231 32 3187
Fragments arranged by size:
3231 3187 1987 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 6298 6298
Size: 8501
XbaI T'CTAG_A
Cuts at: 5532 5532
Size: 8501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1145 2224 2837 5996 1145
Size: 1079 613 3159 3650
Fragments arranged by size:
3650 3159 1079 613
XhoI C'TCGA_G
Cuts at: 5473 5473
Size: 8501
XmaI C'CCGG_G
Cuts at: 1634 1688 3629 4485 5521 1634
Size: 54 1941 856 1036 4614
Fragments arranged by size:
4614 1941 1036 856 54
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AgeI AscI BaeI BsrGI BssHII BstZ17I ClaI
EcoRV FseI HpaI MfeI NdeI NotI NruI PacI
PmlI PshAI PsiI PspXI PsrI SacII SexAI SgrAI
SspI SwaI XmnI ZraI