Restriction map for
pF1KA0914
(Circular) (Six-base) MAPSORT of: pF1KA0914.seq Check: 4703 from: 1 to: 5120
pF1KA0914 5120 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1131 1605 1131
Size: 474 4646
AccI GT'mk_AC
Cuts at: 2158 2158
Size: 5120
Acc65I G'GTAC_C
Cuts at: 1640 2136 1640
Size: 496 4624
AclI AA'CG_TT
Cuts at: 4960 4960
Size: 5120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1331 2045 2945 3377 4124 4392 1331
Size: 714 900 432 747 268 2059
Fragments arranged by size:
2059 900 747 714 432 268
AflIII A'CryG_T
Cuts at: 2472 3576 4553 2472
Size: 1104 977 3039
Fragments arranged by size:
3039 1104 977
AgeI A'CCGG_T
Cuts at: 3508 3508
Size: 5120
AleI CACnn'nnGTG
Cuts at: 1030 1030
Size: 5120
AlwNI CAG_nnn'CTG
Cuts at: 310 951 2425 3992 310
Size: 641 1474 1567 1438
Fragments arranged by size:
1567 1474 1438 641
ApaLI G'TGCA_C
Cuts at: 1766 3890 1766
Size: 2124 2996
ApoI r'AATT_y
Cuts at: 1270 2124 4473 4729 1270
Size: 854 2349 256 1661
Fragments arranged by size:
2349 1661 854 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5120
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
AvaI C'yCGr_G
Cuts at: 1667 2140 1667
Size: 473 4647
BamHI G'GATC_C
Cuts at: 2145 2145
Size: 5120
BanI G'GyrC_C
Cuts at: 932 1640 2136 2550 2798 2833 932
Size: 708 496 414 248 35 3219
Fragments arranged by size:
3219 708 496 414 248 35
BanII G_rGCy'C
Cuts at: 712 2134 3164 712
Size: 1422 1030 2668
Fragments arranged by size:
2668 1422 1030
BbeI G_GCGC'C
Cuts at: 936 2554 2802 936
Size: 1618 248 3254
Fragments arranged by size:
3254 1618 248
BbsI GAAGACnn'nnnn_
Cuts at: 965 1718 1970 2012 965
Size: 753 252 42 4073
Fragments arranged by size:
4073 753 252 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1747 1781 1747
Size: 34 5086
BciVI GTATCCnnnnn_n'
Cuts at: 3018 3779 5120 3018
Size: 761 1341 3018
Fragments arranged by size:
3018 1341 761
BclI T'GATC_A
Cuts at: 2640 2640
Size: 5120
BglII A'GATC_T
Cuts at: 2635 4672 2635
Size: 2037 3083
BlpI GC'TnA_GC
Cuts at: 2232 2232
Size: 5120
Bme1580I G_kGCm'C
Cuts at: 812 1770 2745 2838 3894 812
Size: 958 975 93 1056 2038
Fragments arranged by size:
2038 1056 975 958 93
BmrI ACTGGGnnnn_n'
Cuts at: 807 858 1424 2332 2413 2748 4892 807
Size: 51 566 908 81 335 2144 1035
Fragments arranged by size:
2144 1035 908 566 335 81 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3487 3487
Size: 5120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 530 1453 1894 2092 302
Size: 228 923 441 198 3330
Fragments arranged by size:
3330 923 441 228 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1399 2296 3175 3667 3965 1399
Size: 897 879 492 298 2554
Fragments arranged by size:
2554 897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4824 4824
Size: 5120
BsaAI yAC'GTr
Cuts at: 2473 2473
Size: 5120
BsaBI GATnn'nnATC
Cuts at: 6 2634 4671 6
Size: 2628 2037 455
Fragments arranged by size:
2628 2037 455
BsaHI Gr'CG_yC
Cuts at: 933 2551 2799 3501 4997 933
Size: 1618 248 702 1496 1056
Fragments arranged by size:
1618 1496 1056 702 248
BsaWI w'CCGG_w
Cuts at: 214 1166 1236 1274 2309 2830 3508 3782 3929
Size: 952 70 38 1035 521 678 274 147
Cuts at: 3929 4340 214
Size: 411 994
Fragments arranged by size:
1035 994 952 678 521 411 274 147
70 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1880 1910 2051 2081 1880
Size: 30 141 30 4919
Fragments arranged by size:
4919 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 802 864 971 1071 1714 802
Size: 62 107 100 643 4208
Fragments arranged by size:
4208 643 107 100 62
BseYI C'CCAG_C
Cuts at: 736 928 1127 2545 3303 3880 4376 736
Size: 192 199 1418 758 577 496 1480
Fragments arranged by size:
1480 1418 758 577 496 199 192
BsiEI CG_ry'CG
Cuts at: 104 1594 2662 2708 3916 104
Size: 1490 1068 46 1208 1308
Fragments arranged by size:
1490 1308 1208 1068 46
BsiHKAI G_wGCw'C
Cuts at: 183 1770 2081 2134 2912 3102 3894 183
Size: 1587 311 53 778 190 792 1409
Fragments arranged by size:
1587 1409 792 778 311 190 53
BsmI GAATG_Cn'
Cuts at: 1580 1580
Size: 5120
Bsp1286I G_dGCh'C
Cuts at: 183 712 812 1770 2081 2134 2745 2838 2912
Size: 529 100 958 311 53 611 93 74
Cuts at: 2912 3102 3164 3894 183
Size: 190 62 730 1409
Fragments arranged by size:
1409 958 730 611 529 311 190 100
93 74 62 53
BspEI T'CCGG_A
Cuts at: 1166 1236 1274 4340 1166
Size: 70 38 3066 1946
Fragments arranged by size:
3066 1946 70 38
BspHI T'CATG_A
Cuts at: 564 618 1064 4296 564
Size: 54 446 3232 1388
Fragments arranged by size:
3232 1388 446 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1131 1605 2170 2384 2686 3067 1131
Size: 474 565 214 302 381 3184
Fragments arranged by size:
3184 565 474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1483 3412 3466 4950 5115 50
Size: 1433 1929 54 1484 165 55
Fragments arranged by size:
1929 1484 1433 165 55 54
BsrDI GCAATG_nn'
Cuts at: 207 1728 3032 207
Size: 1521 1304 2295
Fragments arranged by size:
2295 1521 1304
BsrFI r'CCGG_y
Cuts at: 775 1593 1643 3118 3299 3508 775
Size: 818 50 1475 181 209 2387
Fragments arranged by size:
2387 1475 818 209 181 50
BssSI C'ACGA_G
Cuts at: 3391 3749 3391
Size: 358 4762
BstAPI GCAn_nnn'nTGC
Cuts at: 331 2497 331
Size: 2166 2954
BstBI TT'CG_AA
Cuts at: 1187 1790 1941 3481 1187
Size: 603 151 1540 2826
Fragments arranged by size:
2826 1540 603 151
BstEII G'GTnAC_C
Cuts at: 778 778
Size: 5120
BstXI CCAn_nnnn'nTGG
Cuts at: 2112 2112
Size: 5120
BstYI r'GATC_y
Cuts at: 1 722 1163 1658 2145 2612 2635 2970 3216
Size: 721 441 495 487 467 23 335 246
Cuts at: 3216 4228 4314 4326 4672 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 721 495 487 467 449 441 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1499 1499
Size: 5120
BtgI C'CryG_G
Cuts at: 107 2111 107
Size: 2004 3116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3070 3251 3414 3070
Size: 181 163 4776
Fragments arranged by size:
4776 181 163
BtsI GCAGTG_nn'
Cuts at: 2363 2363
Size: 5120
DraI TTT'AAA
Cuts at: 2119 2119
Size: 5120
DrdI GACnn_nn'nnGTC
Cuts at: 1741 2826 3684 1741
Size: 1085 858 3177
Fragments arranged by size:
3177 1085 858
EaeI y'GGCC_r
Cuts at: 1591 2705 2879 3270 3297 4642 1591
Size: 1114 174 391 27 1345 2069
Fragments arranged by size:
2069 1345 1114 391 174 27
EagI C'GGCC_G
Cuts at: 1591 2705 1591
Size: 1114 4006
EarI CTCTTCn'nnn_
Cuts at: 848 1187 1307 1691 3143 3353 848
Size: 339 120 384 1452 210 2615
Fragments arranged by size:
2615 1452 384 339 210 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3328 3638 3784 4930 3328
Size: 310 146 1146 3518
Fragments arranged by size:
3518 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2132 2132
Size: 5120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 530 1331 1453 1894 2045 2092 2945 3377
Size: 228 801 122 441 151 47 853 432
Cuts at: 3377 4124 4392 302
Size: 747 268 1030
Fragments arranged by size:
1030 853 801 747 441 432 268 228
151 122 47
EcoO109I rG'GnC_Cy
Cuts at: 1091 1113 1317 2259 1091
Size: 22 204 942 3952
Fragments arranged by size:
3952 942 204 22
EcoRI G'AATT_C
Cuts at: 2124 2124
Size: 5120
EcoRV GAT'ATC
Cuts at: 1307 1307
Size: 5120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1752 3139 1752
Size: 1387 3733
FspI TGC'GCA
Cuts at: 2901 2901
Size: 5120
HaeII r_GCGC'y
Cuts at: 354 936 2554 2802 3824 4803 354
Size: 582 1618 248 1022 979 671
Fragments arranged by size:
1618 1022 979 671 582 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 292 324 1690 1722 2092 2124 2962 2992 2994
Size: 32 1366 32 370 32 838 30 2
Cuts at: 2994 3024 292
Size: 30 2388
Fragments arranged by size:
2388 1366 838 370 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2159 2159
Size: 5120
KasI G'GCGC_C
Cuts at: 932 2550 2798 932
Size: 1618 248 3254
Fragments arranged by size:
3254 1618 248
KpnI G_GTAC'C
Cuts at: 1644 2140 1644
Size: 496 4624
MluI A'CGCG_T
Cuts at: 4553 4553
Size: 5120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 797 3791 3975 797
Size: 2994 184 1942
Fragments arranged by size:
2994 1942 184
MscI TGG'CCA
Cuts at: 2881 2881
Size: 5120
MslI CAynn'nnrTG
Cuts at: 446 1030 1842 2017 3109 3236 446
Size: 584 812 175 1092 127 2330
Fragments arranged by size:
2330 1092 812 584 175 127
MspA1I CmG'CkG
Cuts at: 247 885 948 2231 2545 2905 3918 4163 247
Size: 638 63 1283 314 360 1013 245 1204
Fragments arranged by size:
1283 1204 1013 638 360 314 245 63
NaeI GCC'GGC
Cuts at: 3301 3301
Size: 5120
NarI GG'CG_CC
Cuts at: 933 2551 2799 933
Size: 1618 248 3254
Fragments arranged by size:
3254 1618 248
NcoI C'CATG_G
Cuts at: 107 2111 107
Size: 2004 3116
NgoMIV G'CCGG_C
Cuts at: 3299 3299
Size: 5120
NspI r_CATG'y
Cuts at: 2173 2673 3580 2173
Size: 500 907 3713
Fragments arranged by size:
3713 907 500
PciI A'CATG_T
Cuts at: 3576 3576
Size: 5120
PfoI T'CCnGG_A
Cuts at: 725 1469 1898 1910 2069 725
Size: 744 429 12 159 3776
Fragments arranged by size:
3776 744 429 159 12
PmeI GTTT'AAAC
Cuts at: 2119 2119
Size: 5120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 883 915 1353 1385 4259 4291 883
Size: 32 438 32 2874 32 1712
Fragments arranged by size:
2874 1712 438 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1091 1113 1317 1091
Size: 22 204 4894
Fragments arranged by size:
4894 204 22
PshAI GACnn'nnGTC
Cuts at: 159 159
Size: 5120
PstI C_TGCA'G
Cuts at: 1556 2167 2852 1556
Size: 611 685 3824
Fragments arranged by size:
3824 685 611
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5120
PvuII CAG'CTG
Cuts at: 247 948 2545 2905 247
Size: 701 1597 360 2462
Fragments arranged by size:
2462 1597 701 360
RsrII CG'GwC_CG
Cuts at: 3315 3315
Size: 5120
SacI G_AGCT'C
Cuts at: 2134 2134
Size: 5120
SalI G'TCGA_C
Cuts at: 2157 2157
Size: 5120
SapI GCTCTTCn'nnn_
Cuts at: 848 3143 3353 848
Size: 2295 210 2615
Fragments arranged by size:
2615 2295 210
SbfI CC_TGCA'GG
Cuts at: 2167 2167
Size: 5120
ScaI AGT'ACT
Cuts at: 546 4637 546
Size: 4091 1029
SfcI C'TryA_G
Cuts at: 33 248 1552 2163 2848 3841 4032 33
Size: 215 1304 611 685 993 191 1121
Fragments arranged by size:
1304 1121 993 685 611 215 191
SfoI GGC'GCC
Cuts at: 934 2552 2800 934
Size: 1618 248 3254
Fragments arranged by size:
3254 1618 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5120
SmaI CCC'GGG
Cuts at: 2142 2142
Size: 5120
SmlI C'TyrA_G
Cuts at: 1378 2275 3190 3682 3944 1378
Size: 897 915 492 262 2554
Fragments arranged by size:
2554 915 897 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5120
SphI G_CATG'C
Cuts at: 2173 2673 2173
Size: 500 4620
StyI C'CwwG_G
Cuts at: 107 365 2111 2254 2608 107
Size: 258 1746 143 354 2619
Fragments arranged by size:
2619 1746 354 258 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2703 2836 3295 3504 2703
Size: 133 459 209 4319
Fragments arranged by size:
4319 459 209 133
TatI w'GTAC_w
Cuts at: 544 1202 4635 544
Size: 658 3433 1029
Fragments arranged by size:
3433 1029 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4637 4669 4637
Size: 32 5088
Tth111I GACn'n_nGTC
Cuts at: 2917 2917
Size: 5120
XbaI T'CTAG_A
Cuts at: 2151 2151
Size: 5120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2615 2615
Size: 5120
XmaI C'CCGG_G
Cuts at: 2140 2140
Size: 5120
XmnI GAAnn'nnTTC
Cuts at: 1454 1580 1454
Size: 126 4994
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI