Restriction map for
pF1KA0917
(Circular) (Six-base) MAPSORT of: pF1KA0917.seq Check: 5014 from: 1 to: 5039
pF1KA0917 5039 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 374 2077 374
Size: 1703 3336
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 4879 4879
Size: 5039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 983 2864 3296 3649 4311 413
Size: 570 1881 432 353 662 1141
Fragments arranged by size:
1881 1141 662 570 432 353
AflIII A'CryG_T
Cuts at: 2391 4191 4472 2391
Size: 1800 281 2958
Fragments arranged by size:
2958 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1441 2344 3782 1441
Size: 903 1438 2698
Fragments arranged by size:
2698 1438 903
ApaLI G'TGCA_C
Cuts at: 949 3877 949
Size: 2928 2111
ApoI r'AATT_y
Cuts at: 189 456 1078 2043 4392 4648 189
Size: 267 622 965 2349 256 580
Fragments arranged by size:
2349 965 622 580 267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5039
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 2059 2059
Size: 5039
BamHI G'GATC_C
Cuts at: 2064 2064
Size: 5039
BanI G'GyrC_C
Cuts at: 2055 2469 2717 2752 2055
Size: 414 248 35 4342
Fragments arranged by size:
4342 414 248 35
BanII G_rGCy'C
Cuts at: 2053 3083 2053
Size: 1030 4009
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbvCI CC'TCA_GC
Cuts at: 1617 1617
Size: 5039
BciVI GTATCCnnnnn_n'
Cuts at: 2937 3993 5039 2937
Size: 1056 1046 2937
Fragments arranged by size:
2937 1056 1046
BclI T'GATC_A
Cuts at: 2559 2559
Size: 5039
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5039
BglII A'GATC_T
Cuts at: 2554 4591 2554
Size: 2037 3002
BlpI GC'TnA_GC
Cuts at: 1247 2151 1247
Size: 904 4135
Bme1580I G_kGCm'C
Cuts at: 953 2664 2757 3881 953
Size: 1711 93 1124 2111
Fragments arranged by size:
2111 1711 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2251 2332 2667 4811 2251
Size: 81 335 2144 2479
Fragments arranged by size:
2479 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1001 1439 1001
Size: 438 4601
Bpu10I CC'TnA_GC
Cuts at: 1617 1617
Size: 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 2215 3094 3808 4106 1306
Size: 909 879 714 298 2239
Fragments arranged by size:
2239 909 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4743 4743
Size: 5039
BsaAI yAC'GTr
Cuts at: 2392 2392
Size: 5039
BsaBI GATnn'nnATC
Cuts at: 6 347 2553 4590 6
Size: 341 2206 2037 455
Fragments arranged by size:
2206 2037 455 341
BsaHI Gr'CG_yC
Cuts at: 2470 2718 3420 4916 2470
Size: 248 702 1496 2593
Fragments arranged by size:
2593 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1068 2228 2749 3427 3838 3985 4259 1068
Size: 1160 521 678 411 147 274 1848
Fragments arranged by size:
1848 1160 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 1765 1795 215
Size: 30 1520 30 3459
Fragments arranged by size:
3459 1520 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 269 1913 269
Size: 1644 3395
BseYI C'CCAG_C
Cuts at: 2464 3222 3887 4295 2464
Size: 758 665 408 3208
Fragments arranged by size:
3208 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 5039
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3857 104
Size: 2477 46 1230 1286
Fragments arranged by size:
2477 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 953 1158 2053 2831 3021 3881 953
Size: 205 895 778 190 860 2111
Fragments arranged by size:
2111 895 860 778 205 190
Bsp1286I G_dGCh'C
Cuts at: 953 1158 2053 2664 2757 2831 3021 3083 3881
Size: 205 895 611 93 74 190 62 798
Cuts at: 3881 953
Size: 2111
Fragments arranged by size:
2111 895 798 611 205 190 93 74
62
BspHI T'CATG_A
Cuts at: 1676 3471 1676
Size: 1795 3244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2089 2303 2605 2986 2089
Size: 214 302 381 4142
Fragments arranged by size:
4142 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3331 3385 4869 5034 50
Size: 3281 54 1484 165 55
Fragments arranged by size:
3281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1842 2951 1842
Size: 1109 3930
BsrFI r'CCGG_y
Cuts at: 3037 3218 3037
Size: 181 4858
BssSI C'ACGA_G
Cuts at: 1670 3310 4018 1670
Size: 1640 708 2691
Fragments arranged by size:
2691 1640 708
BstAPI GCAn_nnn'nTGC
Cuts at: 520 2416 520
Size: 1896 3143
BstBI TT'CG_AA
Cuts at: 426 3400 426
Size: 2974 2065
BstYI r'GATC_y
Cuts at: 1 348 420 2064 2531 2554 2889 3135 3441
Size: 347 72 1644 467 23 335 246 306
Cuts at: 3441 3453 3539 4591 1
Size: 12 86 1052 449
Fragments arranged by size:
1644 1052 467 449 347 335 306 246
86 72 23 12
BstZ17I GTA'TAC
Cuts at: 375 375
Size: 5039
BtgI C'CryG_G
Cuts at: 107 107
Size: 5039
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 779 1325 1983 2282 779
Size: 546 658 299 3536
Fragments arranged by size:
3536 658 546 299
DraI TTT'AAA
Cuts at: 455 974 2038 455
Size: 519 1064 3456
Fragments arranged by size:
3456 1064 519
DrdI GACnn_nn'nnGTC
Cuts at: 2745 4089 2745
Size: 1344 3695
EaeI y'GGCC_r
Cuts at: 261 852 1625 2624 2798 3189 3216 4561 261
Size: 591 773 999 174 391 27 1345 739
Fragments arranged by size:
1345 999 773 739 591 391 174 27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 703 1424 3062 3272 703
Size: 721 1638 210 2470
Fragments arranged by size:
2470 1638 721 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3247 3989 4135 4849 3247
Size: 742 146 714 3437
Fragments arranged by size:
3437 742 714 146
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 983 1001 1439 2864 3296 3649 4311 413
Size: 570 18 438 1425 432 353 662 1141
Fragments arranged by size:
1425 1141 662 570 438 432 353 18
EcoNI CCTnn'n_nnAGG
Cuts at: 1445 1445
Size: 5039
EcoO109I rG'GnC_Cy
Cuts at: 872 2178 872
Size: 1306 3733
EcoRI G'AATT_C
Cuts at: 2043 2043
Size: 5039
EcoRV GAT'ATC
Cuts at: 648 648
Size: 5039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 5039
FspI TGC'GCA
Cuts at: 2820 2820
Size: 5039
HaeII r_GCGC'y
Cuts at: 2473 2721 3951 4722 2473
Size: 248 1230 771 2790
Fragments arranged by size:
2790 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 259 291 1417 1449 1765 1797 2881 2911 2913
Size: 32 1126 32 316 32 1084 30 2
Cuts at: 2913 2943 259
Size: 30 2355
Fragments arranged by size:
2355 1126 1084 316 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 690 891 1932 2078 690
Size: 201 1041 146 3651
Fragments arranged by size:
3651 1041 201 146
HindIII A'AGCT_T
Cuts at: 1541 1541
Size: 5039
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3798 3982 3798
Size: 184 4855
MscI TGG'CCA
Cuts at: 263 854 2800 263
Size: 591 1946 2502
Fragments arranged by size:
2502 1946 591
MslI CAynn'nnrTG
Cuts at: 661 1225 1316 3028 3155 661
Size: 564 91 1712 127 2545
Fragments arranged by size:
2545 1712 564 127 91
MspA1I CmG'CkG
Cuts at: 1021 1261 2150 2464 2824 3608 3853 1021
Size: 240 889 314 360 784 245 2207
Fragments arranged by size:
2207 889 784 360 314 245 240
NaeI GCC'GGC
Cuts at: 3220 3220
Size: 5039
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NcoI C'CATG_G
Cuts at: 107 107
Size: 5039
NgoMIV G'CCGG_C
Cuts at: 3218 3218
Size: 5039
NspI r_CATG'y
Cuts at: 2092 2592 4195 2092
Size: 500 1603 2936
Fragments arranged by size:
2936 1603 500
PciI A'CATG_T
Cuts at: 4191 4191
Size: 5039
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 3485
Size: 32 5007
PstI C_TGCA'G
Cuts at: 372 1978 2086 2771 372
Size: 1606 108 685 2640
Fragments arranged by size:
2640 1606 685 108
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 1021 1261 2464 2824 1021
Size: 240 1203 360 3236
Fragments arranged by size:
3236 1203 360 240
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5039
SalI G'TCGA_C
Cuts at: 2076 2076
Size: 5039
SapI GCTCTTCn'nnn_
Cuts at: 3062 3272 3062
Size: 210 4829
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 4556 4556
Size: 5039
SexAI A'CCwGG_T
Cuts at: 1718 1718
Size: 5039
SfcI C'TryA_G
Cuts at: 33 368 683 1803 1974 2082 2767 3735 3926
Size: 335 315 1120 171 108 685 968 191
Cuts at: 3926 33
Size: 1146
Fragments arranged by size:
1146 1120 968 685 335 315 191 171
108
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 1285 2194 3109 3823 4085 1285
Size: 909 915 714 262 2239
Fragments arranged by size:
2239 915 909 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5039
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
SspI AAT'ATT
Cuts at: 402 402
Size: 5039
StyI C'CwwG_G
Cuts at: 107 539 2173 2527 107
Size: 432 1634 354 2619
Fragments arranged by size:
2619 1634 432 354
SwaI ATTT'AAAT
Cuts at: 455 455
Size: 5039
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 709 2622 2755 3214 3423 709
Size: 1913 133 459 209 2325
Fragments arranged by size:
2325 1913 459 209 133
TatI w'GTAC_w
Cuts at: 957 4554 957
Size: 3597 1442
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4556 4588 4556
Size: 32 5007
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 1422 2070 1422
Size: 648 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1653 1887 2534 1653
Size: 234 647 4158
Fragments arranged by size:
4158 647 234
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
XmnI GAAnn'nnTTC
Cuts at: 1127 1127
Size: 5039
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstEII BstXI Bsu36I ClaI CspCI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI XhoI ZraI