Restriction map for
pF1KA0925
(Circular) (Six-base) MAPSORT of: pF1KA0925.seq Check: 816 from: 1 to: 4538
pF1KA0925 4538 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 152 1576 152
Size: 1424 3114
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 478 2363 2795 3542 3810 478
Size: 1885 432 747 268 1206
Fragments arranged by size:
1885 1206 747 432 268
AfeI AGC'GCT
Cuts at: 558 558
Size: 4538
AflIII A'CryG_T
Cuts at: 419 1890 2994 3971 419
Size: 1471 1104 977 986
Fragments arranged by size:
1471 1104 986 977
AgeI A'CCGG_T
Cuts at: 2926 2926
Size: 4538
AleI CACnn'nnGTG
Cuts at: 227 424 634 227
Size: 197 210 4131
Fragments arranged by size:
4131 210 197
AlwNI CAG_nnn'CTG
Cuts at: 1843 3410 1843
Size: 1567 2971
ApaLI G'TGCA_C
Cuts at: 211 3308 211
Size: 3097 1441
ApoI r'AATT_y
Cuts at: 1542 3891 4147 1542
Size: 2349 256 1933
Fragments arranged by size:
2349 1933 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4538
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 445 1558 445
Size: 1113 3425
AvrII C'CTAG_G
Cuts at: 1056 1056
Size: 4538
BamHI G'GATC_C
Cuts at: 191 1563 191
Size: 1372 3166
BanI G'GyrC_C
Cuts at: 527 1554 1968 2216 2251 527
Size: 1027 414 248 35 2814
Fragments arranged by size:
2814 1027 414 248 35
BanII G_rGCy'C
Cuts at: 719 902 1148 1406 1552 2582 719
Size: 183 246 258 146 1030 2675
Fragments arranged by size:
2675 1030 258 246 183 146
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BbsI GAAGACnn'nnnn_
Cuts at: 1196 1196
Size: 4538
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3197 4538 2436
Size: 761 1341 2436
Fragments arranged by size:
2436 1341 761
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglI GCCn_nnn'nGGC
Cuts at: 353 353
Size: 4538
BglII A'GATC_T
Cuts at: 2053 4090 2053
Size: 2037 2501
BlpI GC'TnA_GC
Cuts at: 1650 1650
Size: 4538
Bme1580I G_kGCm'C
Cuts at: 215 500 1166 2163 2256 3312 215
Size: 285 666 997 93 1056 1441
Fragments arranged by size:
1441 1056 997 666 285 93
BmgBI CAC'GTC
Cuts at: 422 422
Size: 4538
BmrI ACTGGGnnnn_n'
Cuts at: 1750 1831 2166 4310 1750
Size: 81 335 2144 1978
Fragments arranged by size:
2144 1978 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 738 882 1050 1167 2905 738
Size: 144 168 117 1738 2371
Fragments arranged by size:
2371 1738 168 144 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 505 804 1474 322
Size: 183 299 670 3386
Fragments arranged by size:
3386 670 299 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1114 1714 2593 3085 3383 1114
Size: 600 879 492 298 2269
Fragments arranged by size:
2269 879 600 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 822 4242 822
Size: 3420 1118
BsaAI yAC'GTr
Cuts at: 686 1891 686
Size: 1205 3333
BsaBI GATnn'nnATC
Cuts at: 6 196 2052 4089 6
Size: 190 1856 2037 455
Fragments arranged by size:
2037 1856 455 190
BsaHI Gr'CG_yC
Cuts at: 117 1969 2217 2919 4415 117
Size: 1852 248 702 1496 240
Fragments arranged by size:
1852 1496 702 248 240
BsaWI w'CCGG_w
Cuts at: 143 977 1727 2248 2926 3200 3347 3758 143
Size: 834 750 521 678 274 147 411 923
Fragments arranged by size:
923 834 750 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 118 148 406 436 118
Size: 30 258 30 4220
Fragments arranged by size:
4220 258 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 793 1468 793
Size: 675 3863
BseYI C'CCAG_C
Cuts at: 1038 1245 1963 2721 3298 3794 1038
Size: 207 718 758 577 496 1782
Fragments arranged by size:
1782 758 718 577 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 475
Size: 4538
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 179 215 412 1406 1552 2330 2520 3312 179
Size: 36 197 994 146 778 190 792 1405
Fragments arranged by size:
1405 994 792 778 197 190 146 36
BsmI GAATG_Cn'
Cuts at: 1478 1478
Size: 4538
Bsp1286I G_dGCh'C
Cuts at: 179 215 412 500 719 902 1148 1166 1406
Size: 36 197 88 219 183 246 18 240
Cuts at: 1406 1552 2163 2256 2330 2520 2582 3312 179
Size: 146 611 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 611 246 240 219 197 190
183 146 93 88 74 62 36 18
BspEI T'CCGG_A
Cuts at: 143 3758 143
Size: 3615 923
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 722 1034 2830 2884 4368 4533 50
Size: 672 312 1796 54 1484 165 55
Fragments arranged by size:
1796 1484 672 312 165 55 54
BsrDI GCAATG_nn'
Cuts at: 363 398 2450 363
Size: 35 2052 2451
Fragments arranged by size:
2451 2052 35
BsrFI r'CCGG_y
Cuts at: 503 606 2536 2717 2926 503
Size: 103 1930 181 209 2115
Fragments arranged by size:
2115 1930 209 181 103
BssSI C'ACGA_G
Cuts at: 405 2809 3167 405
Size: 2404 358 1776
Fragments arranged by size:
2404 1776 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 1410 2899 1410
Size: 1489 3049
BstYI r'GATC_y
Cuts at: 1 191 431 440 1263 1563 2030 2053 2388
Size: 190 240 9 823 300 467 23 335
Cuts at: 2388 2634 3646 3732 3744 4090 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 823 467 449 346 335 300 246
240 190 86 23 12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 177 1781 177
Size: 1604 2934
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DraIII CAC_nnn'GTG
Cuts at: 686 765 686
Size: 79 4459
DrdI GACnn_nn'nnGTC
Cuts at: 574 2244 3102 574
Size: 1670 858 2010
Fragments arranged by size:
2010 1670 858
EaeI y'GGCC_r
Cuts at: 164 251 345 726 2123 2297 2688 2715 4060
Size: 87 94 381 1397 174 391 27 1345
Cuts at: 4060 164
Size: 642
Fragments arranged by size:
1397 1345 642 391 381 174 94 87
27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 878 1336 2561 2771 878
Size: 458 1225 210 2645
Fragments arranged by size:
2645 1225 458 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3056 3202 4348 2746
Size: 310 146 1146 2936
Fragments arranged by size:
2936 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1404 1550 1404
Size: 146 4392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 478 505 804 1474 2363 2795 3542 3810
Size: 156 27 299 670 889 432 747 268
Cuts at: 3810 322
Size: 1050
Fragments arranged by size:
1050 889 747 670 432 299 268 156
27
EcoO109I rG'GnC_Cy
Cuts at: 576 861 1134 1677 576
Size: 285 273 543 3437
Fragments arranged by size:
3437 543 285 273
EcoRI G'AATT_C
Cuts at: 1542 1542
Size: 4538
EcoRV GAT'ATC
Cuts at: 369 369
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4538
FspI TGC'GCA
Cuts at: 1513 2319 1513
Size: 806 3732
HaeII r_GCGC'y
Cuts at: 481 560 1972 2220 3242 4221 481
Size: 79 1412 248 1022 979 798
Fragments arranged by size:
1412 1022 979 798 248 79
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 886 918 2380 2410 2412 2442 406
Size: 32 448 32 1462 30 2 30 2502
Fragments arranged by size:
2502 1462 448 32 32 30 30 2
HincII GTy'rAC
Cuts at: 237 1356 1577 237
Size: 1119 221 3198
Fragments arranged by size:
3198 1119 221
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3209 3393 3209
Size: 184 4354
MscI TGG'CCA
Cuts at: 166 253 2299 166
Size: 87 2046 2405
Fragments arranged by size:
2405 2046 87
MslI CAynn'nnrTG
Cuts at: 227 424 632 634 940 1153 1159 2527 2654
Size: 197 208 2 306 213 6 1368 127
Cuts at: 2654 227
Size: 2111
Fragments arranged by size:
2111 1368 306 213 208 197 127 6
2
MspA1I CmG'CkG
Cuts at: 1494 1649 1963 2323 3336 3581 1494
Size: 155 314 360 1013 245 2451
Fragments arranged by size:
2451 1013 360 314 245 155
NaeI GCC'GGC
Cuts at: 505 2719 505
Size: 2214 2324
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NgoMIV G'CCGG_C
Cuts at: 503 2717 503
Size: 2214 2324
NspI r_CATG'y
Cuts at: 651 945 1591 2091 2998 651
Size: 294 646 500 907 2191
Fragments arranged by size:
2191 907 646 500 294
PciI A'CATG_T
Cuts at: 2994 2994
Size: 4538
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4538
PfoI T'CCnGG_A
Cuts at: 482 482
Size: 4538
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PmlI CAC'GTG
Cuts at: 686 686
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PpuMI rG'GwC_Cy
Cuts at: 576 861 576
Size: 285 4253
PshAI GACnn'nnGTC
Cuts at: 814 814
Size: 4538
PstI C_TGCA'G
Cuts at: 740 1585 2270 740
Size: 845 685 3008
Fragments arranged by size:
3008 845 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 1494 1963 2323 1494
Size: 469 360 3709
Fragments arranged by size:
3709 469 360
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 1406 1552 1406
Size: 146 4392
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 4055 4055
Size: 4538
SfcI C'TryA_G
Cuts at: 33 736 1581 2266 3259 3450 33
Size: 703 845 685 993 191 1121
Fragments arranged by size:
1121 993 845 703 685 191
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 1129 1693 2608 3100 3362 1129
Size: 564 915 492 262 2305
Fragments arranged by size:
2305 915 564 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1591 2091 1591
Size: 500 4038
StuI AGG'CCT
Cuts at: 1055 1055
Size: 4538
StyI C'CwwG_G
Cuts at: 335 1056 1672 2026 335
Size: 721 616 354 2847
Fragments arranged by size:
2847 721 616 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 589 952 1401 2121 2254 2713 2922 589
Size: 363 449 720 133 459 209 2205
Fragments arranged by size:
2205 720 459 449 363 209 133
TatI w'GTAC_w
Cuts at: 4053 4053
Size: 4538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4055 4087 4055
Size: 32 4506
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1077 2033 1077
Size: 956 3582
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
XmnI GAAnn'nnTTC
Cuts at: 382 382
Size: 4538
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI EcoNI FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI