Restriction map for
pF1KA0926
(Circular) (Six-base) MAPSORT of: pF1KA0926.seq Check: 9623 from: 1 to: 7400
pF1KA0926 7400 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1134 1134
Size: 7400
AccI GT'mk_AC
Cuts at: 1761 4438 1761
Size: 2677 4723
Acc65I G'GTAC_C
Cuts at: 4416 4416
Size: 7400
AclI AA'CG_TT
Cuts at: 2293 7240 2293
Size: 4947 2453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 190 1191 2392 2557 2899 3042 4339 5225
Size: 15 1001 1201 165 342 143 1297 886
Cuts at: 5225 5657 6404 6672 175
Size: 432 747 268 903
Fragments arranged by size:
1297 1201 1001 903 886 747 432 342
268 165 143 15
AflIII A'CryG_T
Cuts at: 2119 4752 5856 6833 2119
Size: 2633 1104 977 2686
Fragments arranged by size:
2686 2633 1104 977
AgeI A'CCGG_T
Cuts at: 3264 5788 3264
Size: 2524 4876
AhdI GACnn_n'nnGTC
Cuts at: 606 606
Size: 7400
AleI CACnn'nnGTG
Cuts at: 451 649 3805 451
Size: 198 3156 4046
Fragments arranged by size:
4046 3156 198
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1127 1127
Size: 7400
AlwNI CAG_nnn'CTG
Cuts at: 186 1302 2235 2706 2772 2802 2847 3018 3513
Size: 1116 933 471 66 30 45 171 495
Cuts at: 3513 4110 4197 4705 6272 186
Size: 597 87 508 1567 1314
Fragments arranged by size:
1567 1314 1116 933 597 508 495 471
171 87 66 45 30
ApaI G_GGCC'C
Cuts at: 1653 3027 3352 1653
Size: 1374 325 5701
Fragments arranged by size:
5701 1374 325
ApaLI G'TGCA_C
Cuts at: 489 2367 3598 6170 489
Size: 1878 1231 2572 1719
Fragments arranged by size:
2572 1878 1719 1231
ApoI r'AATT_y
Cuts at: 746 937 2383 3490 4404 6753 7009 746
Size: 191 1446 1107 914 2349 256 1137
Fragments arranged by size:
2349 1446 1137 1107 914 256 191
AseI AT'TA_AT
Cuts at: 20 20
Size: 7400
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7400
AvaI C'yCGr_G
Cuts at: 1456 4148 4420 1456
Size: 2692 272 4436
Fragments arranged by size:
4436 2692 272
BamHI G'GATC_C
Cuts at: 467 667 4425 467
Size: 200 3758 3442
Fragments arranged by size:
3758 3442 200
BanI G'GyrC_C
Cuts at: 4416 4830 5078 5113 4416
Size: 414 248 35 6703
Fragments arranged by size:
6703 414 248 35
BanII G_rGCy'C
Cuts at: 217 502 1256 1385 1646 1653 2360 2449 2945
Size: 285 754 129 261 7 707 89 496
Cuts at: 2945 3027 3352 3902 4414 5444 217
Size: 82 325 550 512 1030 2173
Fragments arranged by size:
2173 1030 754 707 550 512 496 325
285 261 129 89 82 7
BbeI G_GCGC'C
Cuts at: 4834 5082 4834
Size: 248 7152
BbsI GAAGACnn'nnnn_
Cuts at: 253 1191 3929 253
Size: 938 2738 3724
Fragments arranged by size:
3724 2738 938
BbvCI CC'TCA_GC
Cuts at: 343 677 1835 2660 3044 343
Size: 334 1158 825 384 4699
Fragments arranged by size:
4699 1158 825 384 334
BciVI GTATCCnnnnn_n'
Cuts at: 1068 3096 5298 6059 7400 1068
Size: 2028 2202 761 1341 1068
Fragments arranged by size:
2202 2028 1341 1068 761
BclI T'GATC_A
Cuts at: 1472 4920 1472
Size: 3448 3952
BglI GCCn_nnn'nGGC
Cuts at: 1282 1317 1282
Size: 35 7365
BglII A'GATC_T
Cuts at: 3215 4035 4915 6952 3215
Size: 820 880 2037 3663
Fragments arranged by size:
3663 2037 880 820
BlpI GC'TnA_GC
Cuts at: 236 1250 1790 2566 3575 4198 4512 236
Size: 1014 540 776 1009 623 314 3124
Fragments arranged by size:
3124 1014 1009 776 623 540 314
Bme1580I G_kGCm'C
Cuts at: 493 1653 1667 2371 3027 3352 3602 5025 5118
Size: 1160 14 704 656 325 250 1423 93
Cuts at: 5118 6174 493
Size: 1056 1719
Fragments arranged by size:
1719 1423 1160 1056 704 656 325 250
93 14
BmgBI CAC'GTC
Cuts at: 2828 3207 2828
Size: 379 7021
BmrI ACTGGGnnnn_n'
Cuts at: 1221 2473 4612 4693 5028 7172 1221
Size: 1252 2139 81 335 2144 1449
Fragments arranged by size:
2144 2139 1449 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 285 910 2627 3264 5767 285
Size: 625 1717 637 2503 1918
Fragments arranged by size:
2503 1918 1717 637 625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 570 1416 2275 2644 2768 3049 3602 3709
Size: 378 846 859 369 124 281 553 107
Cuts at: 3709 3916 3938 4049 192
Size: 207 22 111 3543
Fragments arranged by size:
3543 859 846 553 378 369 281 207
124 111 107 22
Bpu10I CC'TnA_GC
Cuts at: 343 677 1835 2660 2722 2944 3044 3182 343
Size: 334 1158 825 62 222 100 138 4561
Fragments arranged by size:
4561 1158 825 334 222 138 100 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2503 3121 3284 4290 4576 5455 5947 6245 2503
Size: 618 163 1006 286 879 492 298 3658
Fragments arranged by size:
3658 1006 879 618 492 298 286 163
BsaI GGTCTCn'nnnn_
Cuts at: 431 1808 2620 3454 4318 7104 431
Size: 1377 812 834 864 2786 727
Fragments arranged by size:
2786 1377 864 834 812 727
BsaAI yAC'GTr
Cuts at: 2397 4753 2397
Size: 2356 5044
BsaBI GATnn'nnATC
Cuts at: 6 4914 6951 6
Size: 4908 2037 455
Fragments arranged by size:
4908 2037 455
BsaHI Gr'CG_yC
Cuts at: 543 2607 4831 5079 5781 7277 543
Size: 2064 2224 248 702 1496 666
Fragments arranged by size:
2224 2064 1496 702 666 248
BsaWI w'CCGG_w
Cuts at: 3264 4286 4589 5110 5788 6062 6209 6620 3264
Size: 1022 303 521 678 274 147 411 4044
Fragments arranged by size:
4044 1022 678 521 411 303 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1263 1269 1293 1299 1263
Size: 6 24 6 7364
Fragments arranged by size:
7364 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 178 562 1036 3688 178
Size: 384 474 2652 3890
Fragments arranged by size:
3890 2652 474 384
BseYI C'CCAG_C
Cuts at: 430 654 662 682 1425 1437 1805 2872 3736
Size: 224 8 20 743 12 368 1067 864
Cuts at: 3736 4243 4825 5583 6160 6656 430
Size: 507 582 758 577 496 1174
Fragments arranged by size:
1174 1067 864 758 743 582 577 507
496 368 224 20 12 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1375 1671 4096 1375
Size: 296 2425 4679
Fragments arranged by size:
4679 2425 296
BsiEI CG_ry'CG
Cuts at: 104 4942 4988 6196 104
Size: 4838 46 1208 1308
Fragments arranged by size:
4838 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 217 493 1256 1385 1646 1946 2360 2371 2738
Size: 276 763 129 261 300 414 11 367
Cuts at: 2738 3602 3902 4414 5192 5382 6174 217
Size: 864 300 512 778 190 792 1443
Fragments arranged by size:
1443 864 792 778 763 512 414 367
300 300 276 261 190 129 11
BsmBI CGTCTCn'nnnn_
Cuts at: 2598 2598
Size: 7400
Bsp1286I G_dGCh'C
Cuts at: 217 493 502 1256 1385 1646 1653 1667 1946
Size: 276 9 754 129 261 7 14 279
Cuts at: 1946 2360 2371 2449 2738 2945 3027 3352 3602
Size: 414 11 78 289 207 82 325 250
Cuts at: 3602 3902 4414 5025 5118 5192 5382 5444 6174
Size: 300 512 611 93 74 190 62 730
Cuts at: 6174 217
Size: 1443
Fragments arranged by size:
1443 754 730 611 512 414 325 300
289 279 276 261 250 207 190 129
93 82 78 74 62 14 11 9
7
BspEI T'CCGG_A
Cuts at: 6620 6620
Size: 7400
BspHI T'CATG_A
Cuts at: 6576 6576
Size: 7400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1134 2900 3317 4450 4664 4966 5347 1134
Size: 1766 417 1133 214 302 381 3187
Fragments arranged by size:
3187 1766 1133 417 381 302 214
BsrBI CCG'CTC
Cuts at: 50 235 1317 5692 5746 7230 7395 50
Size: 185 1082 4375 54 1484 165 55
Fragments arranged by size:
4375 1484 1082 185 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2009 5312 2009
Size: 3303 4097
BsrFI r'CCGG_y
Cuts at: 480 1410 2183 3264 5398 5579 5788 480
Size: 930 773 1081 2134 181 209 2092
Fragments arranged by size:
2134 2092 1081 930 773 209 181
BssSI C'ACGA_G
Cuts at: 4236 5671 6029 4236
Size: 1435 358 5607
Fragments arranged by size:
5607 1435 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2235 2375 4110 4777 2235
Size: 140 1735 667 4858
Fragments arranged by size:
4858 1735 667 140
BstBI TT'CG_AA
Cuts at: 2321 5761 2321
Size: 3440 3960
BstEII G'GTnAC_C
Cuts at: 2523 3474 2523
Size: 951 6449
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 7400
BstYI r'GATC_y
Cuts at: 1 467 667 984 1298 3215 4035 4425 4892
Size: 466 200 317 314 1917 820 390 467
Cuts at: 4892 4915 5250 5496 6508 6594 6606 6952 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1917 1012 820 467 466 449 390 346
335 317 314 246 200 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1879 3836 1879
Size: 1957 5443
BtgI C'CryG_G
Cuts at: 107 805 868 2453 3353 107
Size: 698 63 1585 900 4154
Fragments arranged by size:
4154 1585 900 698 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5350 5531 5694 5350
Size: 181 163 7056
Fragments arranged by size:
7056 181 163
BtsI GCAGTG_nn'
Cuts at: 458 656 1420 2178 2218 2265 2585 4643 458
Size: 198 764 758 40 47 320 2058 3215
Fragments arranged by size:
3215 2058 764 758 320 198 47 40
DraI TTT'AAA
Cuts at: 3669 4399 3669
Size: 730 6670
DraIII CAC_nnn'GTG
Cuts at: 3615 4119 3615
Size: 504 6896
DrdI GACnn_nn'nnGTC
Cuts at: 5106 5964 5106
Size: 858 6542
EaeI y'GGCC_r
Cuts at: 1136 3951 4985 5159 5550 5577 6922 1136
Size: 2815 1034 174 391 27 1345 1614
Fragments arranged by size:
2815 1614 1345 1034 391 174 27
EagI C'GGCC_G
Cuts at: 4985 4985
Size: 7400
EarI CTCTTCn'nnn_
Cuts at: 222 590 1330 2512 2578 5423 5633 222
Size: 368 740 1182 66 2845 210 1989
Fragments arranged by size:
2845 1989 1182 740 368 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 131 1429 5608 5918 6064 7210 131
Size: 1298 4179 310 146 1146 321
Fragments arranged by size:
4179 1298 1146 321 310 146
EcoICRI GAG'CTC
Cuts at: 215 1254 1383 1644 2358 3900 4412 215
Size: 1039 129 261 714 1542 512 3203
Fragments arranged by size:
3203 1542 1039 714 512 261 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 175 190 192 570 1191 1416 2275 2392 2557
Size: 15 2 378 621 225 859 117 165
Cuts at: 2557 2644 2768 2899 3042 3049 3602 3709 3916
Size: 87 124 131 143 7 553 107 207
Cuts at: 3916 3938 4049 4339 5225 5657 6404 6672 175
Size: 22 111 290 886 432 747 268 903
Fragments arranged by size:
903 886 859 747 621 553 432 378
290 268 225 207 165 143 131 124
117 111 107 87 22 15 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 157 1774 3031 157
Size: 1617 1257 4526
Fragments arranged by size:
4526 1617 1257
EcoO109I rG'GnC_Cy
Cuts at: 309 716 800 1539 1650 1801 2217 2475 2673
Size: 407 84 739 111 151 416 258 198
Cuts at: 2673 2844 3023 3024 3096 3306 3348 3349 3358
Size: 171 179 1 72 210 42 1 9
Cuts at: 3358 3553 4539 309
Size: 195 986 3170
Fragments arranged by size:
3170 986 739 416 407 258 210 198
195 179 171 151 111 84 72 42
9 1 1
EcoRI G'AATT_C
Cuts at: 3490 4404 3490
Size: 914 6486
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5419 5419
Size: 7400
FspI TGC'GCA
Cuts at: 5181 5181
Size: 7400
HaeII r_GCGC'y
Cuts at: 4834 5082 6104 7083 4834
Size: 248 1022 979 5151
Fragments arranged by size:
5151 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 251 592 624 724 756 1263 1295 1464
Size: 32 341 32 100 32 507 32 169
Cuts at: 1464 1476 1496 1508 1793 1807 1825 1839 2836
Size: 12 20 12 285 14 18 14 997
Cuts at: 2836 2868 3953 3985 4317 4349 5242 5272 5274
Size: 32 1085 32 332 32 893 30 2
Cuts at: 5274 5304 219
Size: 30 2315
Fragments arranged by size:
2315 1085 997 893 507 341 332 285
169 100 32 32 32 32 32 32
32 30 30 20 18 14 14 12
12 2
HincII GTy'rAC
Cuts at: 1130 3381 4439 1130
Size: 2251 1058 4091
Fragments arranged by size:
4091 2251 1058
HindIII A'AGCT_T
Cuts at: 2404 2404
Size: 7400
KasI G'GCGC_C
Cuts at: 4830 5078 4830
Size: 248 7152
KpnI G_GTAC'C
Cuts at: 4420 4420
Size: 7400
MfeI C'AATT_G
Cuts at: 3224 3224
Size: 7400
MluI A'CGCG_T
Cuts at: 6833 6833
Size: 7400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 6071 6255 6071
Size: 184 7216
MscI TGG'CCA
Cuts at: 1138 3953 5161 1138
Size: 2815 1208 3377
Fragments arranged by size:
3377 2815 1208
MslI CAynn'nnrTG
Cuts at: 262 451 649 3805 5389 5516 262
Size: 189 198 3156 1584 127 2146
Fragments arranged by size:
3156 2146 1584 198 189 127
MspA1I CmG'CkG
Cuts at: 300 525 548 814 948 1173 1211 2232 2412
Size: 225 23 266 134 225 38 1021 180
Cuts at: 2412 2659 2808 2816 3048 3434 3539 3930 4140
Size: 247 149 8 232 386 105 391 210
Cuts at: 4140 4511 4825 5185 6198 6443 300
Size: 371 314 360 1013 245 1257
Fragments arranged by size:
1257 1021 1013 391 386 371 360 314
266 247 245 232 225 225 210 180
149 134 105 38 23 8
NaeI GCC'GGC
Cuts at: 482 1412 2185 5581 482
Size: 930 773 3396 2301
Fragments arranged by size:
3396 2301 930 773
NarI GG'CG_CC
Cuts at: 4831 5079 4831
Size: 248 7152
NcoI C'CATG_G
Cuts at: 107 805 868 2453 107
Size: 698 63 1585 5054
Fragments arranged by size:
5054 1585 698 63
NdeI CA'TA_TG
Cuts at: 2085 2085
Size: 7400
NgoMIV G'CCGG_C
Cuts at: 480 1410 2183 5579 480
Size: 930 773 3396 2301
Fragments arranged by size:
3396 2301 930 773
NspI r_CATG'y
Cuts at: 1198 2337 4453 4953 5860 1198
Size: 1139 2116 500 907 2738
Fragments arranged by size:
2738 2116 1139 907 500
PasI CC'CwG_GG
Cuts at: 495 1667 495
Size: 1172 6228
PciI A'CATG_T
Cuts at: 5856 5856
Size: 7400
PflMI CCAn_nnn'nTGG
Cuts at: 2847 3513 2847
Size: 666 6734
PfoI T'CCnGG_A
Cuts at: 208 2621 3200 3686 208
Size: 2413 579 486 3922
Fragments arranged by size:
3922 2413 579 486
PmeI GTTT'AAAC
Cuts at: 4399 4399
Size: 7400
PmlI CAC'GTG
Cuts at: 2397 2397
Size: 7400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 882 914 3884 3916 6539 6571 882
Size: 32 2970 32 2623 32 1711
Fragments arranged by size:
2970 2623 1711 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 309 716 1539 1801 2217 2475 2673 2844 3306
Size: 407 823 262 416 258 198 171 462
Cuts at: 3306 309
Size: 4403
Fragments arranged by size:
4403 823 462 416 407 262 258 198
171
PspOMI G'GGCC_C
Cuts at: 1649 3023 3348 1649
Size: 1374 325 5701
Fragments arranged by size:
5701 1374 325
PstI C_TGCA'G
Cuts at: 1106 1216 1499 2231 2243 2580 2807 2897 4447
Size: 110 283 732 12 337 227 90 1550
Cuts at: 4447 5132 1106
Size: 685 3374
Fragments arranged by size:
3374 1550 732 685 337 283 227 110
90 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7400
PvuII CAG'CTG
Cuts at: 525 948 1173 1211 2232 2412 2659 2808 2816
Size: 423 225 38 1021 180 247 149 8
Cuts at: 2816 3048 3539 3930 4140 4825 5185 525
Size: 232 491 391 210 685 360 2740
Fragments arranged by size:
2740 1021 685 491 423 391 360 247
232 225 210 180 149 38 8
RsrII CG'GwC_CG
Cuts at: 5595 5595
Size: 7400
SacI G_AGCT'C
Cuts at: 217 1256 1385 1646 2360 3902 4414 217
Size: 1039 129 261 714 1542 512 3203
Fragments arranged by size:
3203 1542 1039 714 512 261 129
SalI G'TCGA_C
Cuts at: 4437 4437
Size: 7400
SanDI GG'GwC_CC
Cuts at: 716 1801 2217 2475 716
Size: 1085 416 258 5641
Fragments arranged by size:
5641 1085 416 258
SapI GCTCTTCn'nnn_
Cuts at: 222 590 1330 5423 5633 222
Size: 368 740 4093 210 1989
Fragments arranged by size:
4093 1989 740 368 210
SbfI CC_TGCA'GG
Cuts at: 4447 4447
Size: 7400
ScaI AGT'ACT
Cuts at: 6917 6917
Size: 7400
SexAI A'CCwGG_T
Cuts at: 275 275
Size: 7400
SfcI C'TryA_G
Cuts at: 33 399 850 1102 1212 1495 1578 1962 2227
Size: 366 451 252 110 283 83 384 265
Cuts at: 2227 2239 2576 2794 2803 2893 3416 4443 5128
Size: 12 337 218 9 90 523 1027 685
Cuts at: 5128 6121 6312 33
Size: 993 191 1121
Fragments arranged by size:
1121 1027 993 685 523 451 384 366
337 283 265 252 218 191 110 90
83 12 9
SfoI GGC'GCC
Cuts at: 4832 5080 4832
Size: 248 7152
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7400
SmaI CCC'GGG
Cuts at: 4422 4422
Size: 7400
SmlI C'TyrA_G
Cuts at: 2518 3136 3299 4269 4555 5470 5962 6224 2518
Size: 618 163 970 286 915 492 262 3694
Fragments arranged by size:
3694 970 915 618 492 286 262 163
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7400
SphI G_CATG'C
Cuts at: 4453 4953 4453
Size: 500 6900
SspI AAT'ATT
Cuts at: 1573 1573
Size: 7400
StuI AGG'CCT
Cuts at: 1070 4018 1070
Size: 2948 4452
StyI C'CwwG_G
Cuts at: 107 805 868 1232 2453 3624 3749 4019 4534
Size: 698 63 364 1221 1171 125 270 515
Cuts at: 4534 4888 107
Size: 354 2619
Fragments arranged by size:
2619 1221 1171 698 515 364 354 270
125 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 508 642 708 881 1659 2725 3505 4983 5116
Size: 134 66 173 778 1066 780 1478 133
Cuts at: 5116 5575 5784 508
Size: 459 209 2124
Fragments arranged by size:
2124 1478 1066 780 778 459 209 173
134 133 66
TatI w'GTAC_w
Cuts at: 6915 6915
Size: 7400
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1269 1301 3060 3092 3262 3294 4276 4308 6917
Size: 32 1759 32 170 32 982 32 2609
Cuts at: 6917 6949 1269
Size: 32 1720
Fragments arranged by size:
2609 1759 1720 982 170 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 5197 5197
Size: 7400
XbaI T'CTAG_A
Cuts at: 4431 4431
Size: 7400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 651 3360 3536 4895 651
Size: 2709 176 1359 3156
Fragments arranged by size:
3156 2709 1359 176
XmaI C'CCGG_G
Cuts at: 4420 4420
Size: 7400
XmnI GAAnn'nnTTC
Cuts at: 175 4261 175
Size: 4086 3314
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI AscI AvrII BaeI BcgI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI NheI NotI NruI
NsiI PacI PshAI PsiI PspXI PsrI SacII SfiI
SgrAI SnaBI SrfI SwaI XhoI ZraI