Restriction map for
pF1KA0929
(Circular) (Six-base) MAPSORT of: pF1KA0929.seq Check: 4340 from: 1 to: 13982
pF1KA0929 13982 bp
With 182 enzymes: SgfI *
November 17, 2015 09:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3250 6727 3250
Size: 3477 10505
AatII G_ACGT'C
Cuts at: 2184 2184
Size: 13982
AccI GT'mk_AC
Cuts at: 5084 7519 11020 5084
Size: 2435 3501 8046
Fragments arranged by size:
8046 3501 2435
Acc65I G'GTAC_C
Cuts at: 10998 10998
Size: 13982
AclI AA'CG_TT
Cuts at: 10823 13822 10823
Size: 2999 10983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 1067 1088 2474 2719 3470 4004 5411 5782
Size: 690 21 1386 245 751 534 1407 371
Cuts at: 5782 8438 8887 10043 10510 10628 11807 12239 12986
Size: 2656 449 1156 467 118 1179 432 747
Cuts at: 12986 13254 377
Size: 268 1105
Fragments arranged by size:
2656 1407 1386 1179 1156 1105 751 747
690 534 467 449 432 371 268 245
118 21
AfeI AGC'GCT
Cuts at: 1363 2335 9332 1363
Size: 972 6997 6013
Fragments arranged by size:
6997 6013 972
AflIII A'CryG_T
Cuts at: 3047 6010 6440 9588 11334 12438 13415 3047
Size: 2963 430 3148 1746 1104 977 3614
Fragments arranged by size:
3614 3148 2963 1746 1104 977 430
AgeI A'CCGG_T
Cuts at: 7299 9902 12370 7299
Size: 2603 2468 8911
Fragments arranged by size:
8911 2603 2468
AleI CACnn'nnGTG
Cuts at: 2668 6031 8113 2668
Size: 3363 2082 8537
Fragments arranged by size:
8537 3363 2082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 9190 10104 10153 9190
Size: 914 49 13019
Fragments arranged by size:
13019 914 49
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 311 343 2361 2393 3747 3779 311
Size: 32 2018 32 1354 32 10514
Fragments arranged by size:
10514 2018 1354 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 756 931 4849 5242 5332 5389 5419 6750 7666
Size: 175 3918 393 90 57 30 1331 916
Cuts at: 7666 9499 9825 10518 10546 11287 12854 756
Size: 1833 326 693 28 741 1567 1884
Fragments arranged by size:
3918 1884 1833 1567 1331 916 741 693
393 326 175 90 57 30 28
ApaI G_GGCC'C
Cuts at: 6040 8051 8999 9384 10015 10619 6040
Size: 2011 948 385 631 604 9403
Fragments arranged by size:
9403 2011 948 631 604 385
ApaLI G'TGCA_C
Cuts at: 6740 9871 12752 6740
Size: 3131 2881 7970
Fragments arranged by size:
7970 3131 2881
ApoI r'AATT_y
Cuts at: 121 1057 1066 1246 1276 3113 3907 3978 4273
Size: 936 9 180 30 1837 794 71 295
Cuts at: 4273 4401 7212 7881 8521 10414 10986 13335 13591
Size: 128 2811 669 640 1893 572 2349 256
Cuts at: 13591 121
Size: 512
Fragments arranged by size:
2811 2349 1893 1837 936 794 669 640
572 512 295 256 180 128 71 30
9
AseI AT'TA_AT
Cuts at: 20 20
Size: 13982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 13982
AvaI C'yCGr_G
Cuts at: 503 659 1940 2015 2627 5197 5558 8719 8991
Size: 156 1281 75 612 2570 361 3161 272
Cuts at: 8991 9076 9344 9451 9485 11002 503
Size: 85 268 107 34 1517 3483
Fragments arranged by size:
3483 3161 2570 1517 1281 612 361 272
268 156 107 85 75 34
AvrII C'CTAG_G
Cuts at: 592 3820 592
Size: 3228 10754
BamHI G'GATC_C
Cuts at: 3804 7448 11007 3804
Size: 3644 3559 6779
Fragments arranged by size:
6779 3644 3559
BanI G'GyrC_C
Cuts at: 4116 4977 5684 6977 9639 10998 11412 11660 11695
Size: 861 707 1293 2662 1359 414 248 35
Cuts at: 11695 4116
Size: 6403
Fragments arranged by size:
6403 2662 1359 1293 861 707 414 248
35
BanII G_rGCy'C
Cuts at: 530 600 977 4754 5753 6040 6064 7465 7515
Size: 70 377 3777 999 287 24 1401 50
Cuts at: 7515 8008 8051 8389 8726 8999 9351 9384 10015
Size: 493 43 338 337 273 352 33 631
Cuts at: 10015 10034 10619 10996 12026 530
Size: 19 585 377 1030 2486
Fragments arranged by size:
3777 2486 1401 1030 999 631 585 493
377 377 352 338 337 287 273 70
50 43 33 24 19
BbeI G_GCGC'C
Cuts at: 4981 5688 6981 11416 11664 4981
Size: 707 1293 4435 248 7299
Fragments arranged by size:
7299 4435 1293 707 248
BbsI GAAGACnn'nnnn_
Cuts at: 2480 3371 3382 4226 4957 5005 6443 8458 2480
Size: 891 11 844 731 48 1438 2015 8004
Fragments arranged by size:
8004 2015 1438 891 844 731 48 11
BbvCI CC'TCA_GC
Cuts at: 3073 6083 6530 6858 9662 10046 3073
Size: 3010 447 328 2804 384 7009
Fragments arranged by size:
7009 3010 2804 447 384 328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1577 1611 3453 3487 1577
Size: 34 1842 34 12072
Fragments arranged by size:
12072 1842 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9799 11880 12641 13982 9799
Size: 2081 761 1341 9799
Fragments arranged by size:
9799 2081 1341 761
BclI T'GATC_A
Cuts at: 3345 11502 3345
Size: 8157 5825
BglI GCCn_nnn'nGGC
Cuts at: 6556 9655 10804 6556
Size: 3099 1149 9734
Fragments arranged by size:
9734 3099 1149
BglII A'GATC_T
Cuts at: 4098 6220 6720 10865 11497 13534 4098
Size: 2122 500 4145 632 2037 4546
Fragments arranged by size:
4546 4145 2122 2037 632 500
BlpI GC'TnA_GC
Cuts at: 5333 11094 5333
Size: 5761 8221
Bme1580I G_kGCm'C
Cuts at: 6040 6744 7268 8051 8134 8999 9179 9384 9875
Size: 704 524 783 83 865 180 205 491
Cuts at: 9875 10015 10251 10619 11607 11700 12756 6040
Size: 140 236 368 988 93 1056 7266
Fragments arranged by size:
7266 1056 988 865 783 704 524 491
368 236 205 180 140 93 83
BmgBI CAC'GTC
Cuts at: 3048 6013 6441 3048
Size: 2965 428 10589
Fragments arranged by size:
10589 2965 428
BmrI ACTGGGnnnn_n'
Cuts at: 614 723 939 3256 3764 6384 6468 9166 9174
Size: 109 216 2317 508 2620 84 2698 8
Cuts at: 9174 9296 9800 11194 11275 11610 13754 614
Size: 122 504 1394 81 335 2144 842
Fragments arranged by size:
2698 2620 2317 2144 1394 842 508 504
335 216 122 109 84 81 8
BmtI G_CTAG'C
Cuts at: 764 1448 764
Size: 684 13298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 12349 12349
Size: 13982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 884 1883 2216 2938 5396 6216 6517 6725
Size: 80 999 333 722 2458 820 301 208
Cuts at: 6725 6821 7514 7560 7733 7802 8096 9174 9802
Size: 96 693 46 173 69 294 1078 628
Cuts at: 9802 9861 10669 804
Size: 59 808 4117
Fragments arranged by size:
4117 2458 1078 999 820 808 722 693
628 333 301 294 208 173 96 80
69 59 46
Bpu10I CC'TnA_GC
Cuts at: 2882 3073 6083 6530 6858 7189 8388 9253 9662
Size: 191 3010 447 328 331 1199 865 409
Cuts at: 9662 10007 10046 10223 2882
Size: 345 39 177 6641
Fragments arranged by size:
6641 3010 1199 865 447 409 345 331
328 191 177 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 1486 2860 3201 3614 5524 5893 7055 7301
Size: 1359 1374 341 413 1910 369 1162 246
Cuts at: 7301 8582 10127 10513 10753 11158 12037 12529 12827
Size: 1281 1545 386 240 405 879 492 298
Cuts at: 12827 127
Size: 1282
Fragments arranged by size:
1910 1545 1374 1359 1282 1281 1162 879
492 413 405 386 369 341 298 246
240
BsaI GGTCTCn'nnnn_
Cuts at: 1690 2123 2745 3848 8884 10458 13686 1690
Size: 433 622 1103 5036 1574 3228 1986
Fragments arranged by size:
5036 3228 1986 1574 1103 622 433
BsaAI yAC'GTr
Cuts at: 4231 10857 11335 4231
Size: 6626 478 6878
Fragments arranged by size:
6878 6626 478
BsaBI GATnn'nnATC
Cuts at: 6 3803 11496 13533 6
Size: 3797 7693 2037 455
Fragments arranged by size:
7693 3797 2037 455
BsaHI Gr'CG_yC
Cuts at: 2181 4978 5318 5685 6978 11413 11661 12363 13859
Size: 2797 340 367 1293 4435 248 702 1496
Cuts at: 13859 2181
Size: 2304
Fragments arranged by size:
4435 2797 2304 1496 1293 702 367 340
248
BsaWI w'CCGG_w
Cuts at: 2267 2379 7299 9902 11171 11692 12370 12644 12791
Size: 112 4920 2603 1269 521 678 274 147
Cuts at: 12791 13202 2267
Size: 411 3047
Fragments arranged by size:
4920 3047 2603 1269 678 521 411 274
147 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 537 567 1361 1391 7512 7542 7558 7588 7731
Size: 30 794 30 6121 30 16 30 143
Cuts at: 7731 7761 8753 8783 10822 10852 537
Size: 30 992 30 2039 30 3667
Fragments arranged by size:
6121 3667 2039 992 794 143 30 30
30 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 737 2154 2256 3641 5546 5887 5890 6100 6103
Size: 1417 102 1385 1905 341 3 210 3
Cuts at: 6103 7154 7157 7324 7516 7904 9050 9332 9469
Size: 1051 3 167 192 388 1146 282 137
Cuts at: 9469 10007 10034 737
Size: 538 27 4685
Fragments arranged by size:
4685 1905 1417 1385 1146 1051 538 388
341 282 210 192 167 137 102 27
3 3 3
BseYI C'CCAG_C
Cuts at: 1506 3223 5252 5382 6684 7289 7369 8044 8956
Size: 1717 2029 130 1302 605 80 675 912
Cuts at: 8956 8998 9006 9109 9362 9414 9440 9938 10073
Size: 42 8 103 253 52 26 498 135
Cuts at: 10073 10100 10197 10378 11407 12165 12742 13238 1506
Size: 27 97 181 1029 758 577 496 2250
Fragments arranged by size:
2250 2029 1717 1302 1029 912 758 675
605 577 498 496 253 181 135 130
103 97 80 52 42 27 26 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 917 1108 3681 6587 8195 8216 9413 9523 9526
Size: 191 2573 2906 1608 21 1197 110 3
Cuts at: 9526 9883 10064 10075 10570 917
Size: 357 181 11 495 4329
Fragments arranged by size:
4329 2906 2573 1608 1197 495 357 191
181 110 21 11 3
BsiEI CG_ry'CG
Cuts at: 104 5932 8370 11524 11570 12778 104
Size: 5828 2438 3154 46 1208 1308
Fragments arranged by size:
5828 3154 2438 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4754 6064 6744 8038 8432 9875 10996 11774 11964
Size: 1310 680 1294 394 1443 1121 778 190
Cuts at: 11964 12756 4754
Size: 792 5980
Fragments arranged by size:
5980 1443 1310 1294 1121 792 778 680
394 190
BsmI GAATG_Cn'
Cuts at: 7335 7986 8145 8187 7335
Size: 651 159 42 13130
Fragments arranged by size:
13130 651 159 42
BsmBI CGTCTCn'nnnn_
Cuts at: 2207 2394 5025 7968 10569 2207
Size: 187 2631 2943 2601 5620
Fragments arranged by size:
5620 2943 2631 2601 187
Bsp1286I G_dGCh'C
Cuts at: 530 600 977 4754 5753 6040 6064 6744 7268
Size: 70 377 3777 999 287 24 680 524
Cuts at: 7268 7465 7515 8008 8038 8051 8134 8389 8432
Size: 197 50 493 30 13 83 255 43
Cuts at: 8432 8726 8999 9179 9351 9384 9875 10015 10034
Size: 294 273 180 172 33 491 140 19
Cuts at: 10034 10251 10619 10996 11607 11700 11774 11964 12026
Size: 217 368 377 611 93 74 190 62
Cuts at: 12026 12756 530
Size: 730 1756
Fragments arranged by size:
3777 1756 999 730 680 611 524 493
491 377 377 368 294 287 273 255
217 197 190 180 172 140 93 83
74 70 62 50 43 33 30 24
19 13
BspEI T'CCGG_A
Cuts at: 2379 13202 2379
Size: 10823 3159
BspHI T'CATG_A
Cuts at: 573 10955 13158 573
Size: 10382 2203 1397
Fragments arranged by size:
10382 2203 1397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3250 3324 5058 5096 5904 6727 10215 10799 11032
Size: 74 1734 38 808 823 3488 584 233
Cuts at: 11032 11246 11548 11929 3250
Size: 214 302 381 5303
Fragments arranged by size:
5303 3488 1734 823 808 584 381 302
233 214 74 38
BsrBI CCG'CTC
Cuts at: 50 510 687 5735 12274 12328 13812 13977 50
Size: 460 177 5048 6539 54 1484 165 55
Fragments arranged by size:
6539 5048 1484 460 177 165 55 54
BsrDI GCAATG_nn'
Cuts at: 7655 7823 8516 10964 11894 7655
Size: 168 693 2448 930 9743
Fragments arranged by size:
9743 2448 930 693 168
BsrFI r'CCGG_y
Cuts at: 5855 6183 6862 7299 8907 9902 11980 12161 12370
Size: 328 679 437 1608 995 2078 181 209
Cuts at: 12370 5855
Size: 7467
Fragments arranged by size:
7467 2078 1608 995 679 437 328 209
181
BssSI C'ACGA_G
Cuts at: 3610 4355 12253 12611 3610
Size: 745 7898 358 4981
Fragments arranged by size:
7898 4981 745 358
BstAPI GCAn_nnn'nTGC
Cuts at: 931 5038 8182 8284 8614 9870 10518 11359 931
Size: 4107 3144 102 330 1256 648 841 3554
Fragments arranged by size:
4107 3554 3144 1256 841 648 330 102
BstBI TT'CG_AA
Cuts at: 1561 1893 4080 4407 12343 1561
Size: 332 2187 327 7936 3200
Fragments arranged by size:
7936 3200 2187 332 327
BstEII G'GTnAC_C
Cuts at: 713 5158 5719 7359 9849 713
Size: 4445 561 1640 2490 4846
Fragments arranged by size:
4846 4445 2490 1640 561
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1366 6033 6489 8511 114
Size: 1252 4667 456 2022 5585
Fragments arranged by size:
5585 4667 2022 1252 456
BstYI r'GATC_y
Cuts at: 1 498 814 2913 3804 4098 4661 5232 6220
Size: 497 316 2099 891 294 563 571 988
Cuts at: 6220 6720 7448 8492 10865 11007 11474 11497 11832
Size: 500 728 1044 2373 142 467 23 335
Cuts at: 11832 12078 13090 13176 13188 13534 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
2373 2099 1044 1012 988 891 728 571
563 500 497 467 449 346 335 316
294 246 142 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1913 5918 6017 7585 1913
Size: 4005 99 1568 8310
Fragments arranged by size:
8310 4005 1568 99
BtgI C'CryG_G
Cuts at: 5714 6065 6506 7507 8123 9559 9620 9855 10469
Size: 351 441 1001 616 1436 61 235 614
Cuts at: 10469 10973 5714
Size: 504 8723
Fragments arranged by size:
8723 1436 1001 616 614 504 441 351
235 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6501 8558 11932 12113 12276 6501
Size: 2057 3374 181 163 8207
Fragments arranged by size:
8207 3374 2057 181 163
BtsI GCAGTG_nn'
Cuts at: 613 864 873 1773 5321 5486 6248 6906 7396
Size: 251 9 900 3548 165 762 658 490
Cuts at: 7396 8183 8204 8285 11225 613
Size: 787 21 81 2940 3370
Fragments arranged by size:
3548 3370 2940 900 787 762 658 490
251 165 81 21 9
ClaI AT'CG_AT
Cuts at: 3473 3473
Size: 13982
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 7638 7673 7638
Size: 35 13947
DraI TTT'AAA
Cuts at: 2963 3905 10981 2963
Size: 942 7076 5964
Fragments arranged by size:
7076 5964 942
DraIII CAC_nnn'GTG
Cuts at: 6918 9391 6918
Size: 2473 11509
DrdI GACnn_nn'nnGTC
Cuts at: 10315 11688 12546 10315
Size: 1373 858 11751
Fragments arranged by size:
11751 1373 858
EaeI y'GGCC_r
Cuts at: 3953 6635 8246 8501 8624 8909 9322 10116 10728
Size: 2682 1611 255 123 285 413 794 612
Cuts at: 10728 11567 11741 12132 12159 13504 3953
Size: 839 174 391 27 1345 4431
Fragments arranged by size:
4431 2682 1611 1345 839 794 612 413
391 285 255 174 123 27
EagI C'GGCC_G
Cuts at: 11567 11567
Size: 13982
EarI CTCTTCn'nnn_
Cuts at: 1103 1827 3890 4353 4532 4988 5506 5864 7541
Size: 724 2063 463 179 456 518 358 1677
Cuts at: 7541 10328 12005 12215 1103
Size: 2787 1677 210 2870
Fragments arranged by size:
2870 2787 2063 1677 1677 724 518 463
456 358 210 179
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 12190 12500 12646 13792 12190
Size: 310 146 1146 12380
Fragments arranged by size:
12380 1146 310 146
EcoICRI GAG'CTC
Cuts at: 4752 6062 10994 4752
Size: 1310 4932 7740
Fragments arranged by size:
7740 4932 1310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 377 804 884 1067 1088 1883 2216 2474 2719
Size: 427 80 183 21 795 333 258 245
Cuts at: 2719 2938 3470 4004 5396 5411 5782 6216 6517
Size: 219 532 534 1392 15 371 434 301
Cuts at: 6517 6725 6821 7514 7560 7733 7802 8096 8438
Size: 208 96 693 46 173 69 294 342
Cuts at: 8438 8887 9174 9802 9861 10043 10510 10628 10669
Size: 449 287 628 59 182 467 118 41
Cuts at: 10669 11807 12239 12986 13254 377
Size: 1138 432 747 268 1105
Fragments arranged by size:
1392 1138 1105 795 747 693 628 534
532 467 449 434 432 427 371 342
333 301 294 287 268 258 245 219
208 183 182 173 118 96 80 69
59 46 41 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 7690 9265 10022 7690
Size: 1575 757 11650
Fragments arranged by size:
11650 1575 757
EcoO109I rG'GnC_Cy
Cuts at: 785 5201 5939 6037 6573 7695 7993 8068 8593
Size: 4416 738 98 536 1122 298 75 525
Cuts at: 8593 8898 8995 9380 9381 9657 10011 10012 10166
Size: 305 97 385 1 276 354 1 154
Cuts at: 10166 10515 10615 10616 11121 785
Size: 349 100 1 505 3646
Fragments arranged by size:
4416 3646 1122 738 536 525 505 385
354 349 305 298 276 154 100 98
97 75 1 1 1
EcoRI G'AATT_C
Cuts at: 3978 7212 10414 10986 3978
Size: 3234 3202 572 6974
Fragments arranged by size:
6974 3234 3202 572
EcoRV GAT'ATC
Cuts at: 639 3693 639
Size: 3054 10928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1063 1198 2707 2872 3133 4342 5636 6973 7649
Size: 135 1509 165 261 1209 1294 1337 676
Cuts at: 7649 7914 10794 12001 1063
Size: 265 2880 1207 3044
Fragments arranged by size:
3044 2880 1509 1337 1294 1209 1207 676
265 261 165 135
FspI TGC'GCA
Cuts at: 11763 11763
Size: 13982
HaeII r_GCGC'y
Cuts at: 1365 2337 2946 4981 5688 6751 6981 8480 9133
Size: 972 609 2035 707 1063 230 1499 653
Cuts at: 9133 9334 11416 11664 12686 13665 1365
Size: 201 2082 248 1022 979 1682
Fragments arranged by size:
2082 2035 1682 1499 1063 1022 979 972
707 653 609 248 230 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 537 569 1104 1136 3037 3069 3587
Size: 32 156 32 535 32 1901 32 518
Cuts at: 3587 3619 4372 4404 6307 6339 6367 6399 6775
Size: 32 753 32 1903 32 28 32 376
Cuts at: 6775 6807 7201 7233 7558 7590 8352 8371 8384
Size: 32 394 32 325 32 762 19 13
Cuts at: 8384 8403 8689 8721 9990 10022 11824 11854 11856
Size: 19 286 32 1269 32 1802 30 2
Cuts at: 11856 11886 349
Size: 30 2445
Fragments arranged by size:
2445 1903 1901 1802 1269 762 753 535
518 394 376 325 286 156 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 28 19 19
13 2
HincII GTy'rAC
Cuts at: 4434 7566 7713 8294 8979 11021 4434
Size: 3132 147 581 685 2042 7395
Fragments arranged by size:
7395 3132 2042 685 581 147
HindIII A'AGCT_T
Cuts at: 2689 4319 5575 8860 2689
Size: 1630 1256 3285 7811
Fragments arranged by size:
7811 3285 1630 1256
KasI G'GCGC_C
Cuts at: 4977 5684 6977 11412 11660 4977
Size: 707 1293 4435 248 7299
Fragments arranged by size:
7299 4435 1293 707 248
KpnI G_GTAC'C
Cuts at: 11002 11002
Size: 13982
MluI A'CGCG_T
Cuts at: 13415 13415
Size: 13982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3801 5215 7085 7273 10961 12653 12837 3801
Size: 1414 1870 188 3688 1692 184 4946
Fragments arranged by size:
4946 3688 1870 1692 1414 188 184
MscI TGG'CCA
Cuts at: 3955 8626 11743 3955
Size: 4671 3117 6194
Fragments arranged by size:
6194 4671 3117
MslI CAynn'nnrTG
Cuts at: 112 2668 3797 4759 6031 8113 8356 9151 9172
Size: 2556 1129 962 1272 2082 243 795 21
Cuts at: 9172 9587 11971 12098 112
Size: 415 2384 127 1996
Fragments arranged by size:
2556 2384 2082 1996 1272 1129 962 795
415 243 127 21
MspA1I CmG'CkG
Cuts at: 800 1687 1761 3330 5073 5476 5812 5956 8167
Size: 887 74 1569 1743 403 336 144 2211
Cuts at: 8167 8212 8469 8823 9037 9354 9418 9622 9881
Size: 45 257 354 214 317 64 204 259
Cuts at: 9881 10003 10201 10543 10662 11093 11407 11767 12780
Size: 122 198 342 119 431 314 360 1013
Cuts at: 12780 13025 800
Size: 245 1757
Fragments arranged by size:
2211 1757 1743 1569 1013 887 431 403
360 354 342 336 317 314 259 257
245 214 204 198 144 122 119 74
64 45
NaeI GCC'GGC
Cuts at: 5857 6864 8909 12163 5857
Size: 1007 2045 3254 7676
Fragments arranged by size:
7676 3254 2045 1007
NarI GG'CG_CC
Cuts at: 4978 5685 6978 11413 11661 4978
Size: 707 1293 4435 248 7299
Fragments arranged by size:
7299 4435 1293 707 248
NcoI C'CATG_G
Cuts at: 5714 6065 9855 5714
Size: 351 3790 9841
Fragments arranged by size:
9841 3790 351
NgoMIV G'CCGG_C
Cuts at: 5855 6862 8907 12161 5855
Size: 1007 2045 3254 7676
Fragments arranged by size:
7676 3254 2045 1007
NheI G'CTAG_C
Cuts at: 760 1444 760
Size: 684 13298
NruI TCG'CGA
Cuts at: 602 602
Size: 13982
NspI r_CATG'y
Cuts at: 1197 1527 9592 11035 11535 12442 1197
Size: 330 8065 1443 500 907 2737
Fragments arranged by size:
8065 2737 1443 907 500 330
PasI CC'CwG_GG
Cuts at: 6290 8344 8757 8784 9267 9614 6290
Size: 2054 413 27 483 347 10658
Fragments arranged by size:
10658 2054 483 413 347 27
PciI A'CATG_T
Cuts at: 9588 12438 9588
Size: 2850 11132
PflMI CCAn_nnn'nTGG
Cuts at: 10508 10508
Size: 13982
PfoI T'CCnGG_A
Cuts at: 2193 6702 6800 9988 2193
Size: 4509 98 3188 6187
Fragments arranged by size:
6187 4509 3188 98
PmeI GTTT'AAAC
Cuts at: 10981 10981
Size: 13982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13121 13153 13121
Size: 32 13950
PpuMI rG'GwC_Cy
Cuts at: 785 5939 8898 10166 785
Size: 5154 2959 1268 4601
Fragments arranged by size:
5154 4601 2959 1268
PshAI GACnn'nnGTC
Cuts at: 8369 8896 9598 8369
Size: 527 702 12753
Fragments arranged by size:
12753 702 527
PspOMI G'GGCC_C
Cuts at: 6036 8047 8995 9380 10011 10615 6036
Size: 2011 948 385 631 604 9403
Fragments arranged by size:
9403 2011 948 631 604 385
PspXI vC'TCGA_Gb
Cuts at: 1940 2627 9485 1940
Size: 687 6858 6437
Fragments arranged by size:
6858 6437 687
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1391 1423 4261 4293 1391
Size: 32 2838 32 11080
Fragments arranged by size:
11080 2838 32 32
PstI C_TGCA'G
Cuts at: 924 2895 3335 5328 5481 5640 5811 5901 5955
Size: 1971 440 1993 153 159 171 90 54
Cuts at: 5955 6303 6755 7416 8610 9165 9359 10040 10796
Size: 348 452 661 1194 555 194 681 756
Cuts at: 10796 10865 11029 11714 924
Size: 69 164 685 3192
Fragments arranged by size:
3192 1993 1971 1194 756 685 681 661
555 452 440 348 194 171 164 159
153 90 69 54
PvuI CG_AT'CG
Cuts at: 104 8370 104
Size: 8266 5716
PvuII CAG'CTG
Cuts at: 1687 1761 3330 5073 5476 5956 8823 9037 9418
Size: 74 1569 1743 403 480 2867 214 381
Cuts at: 9418 10003 10201 10543 10662 11407 11767 1687
Size: 585 198 342 119 745 360 3902
Fragments arranged by size:
3902 2867 1743 1569 745 585 480 403
381 360 342 214 198 119 74
RsrII CG'GwC_CG
Cuts at: 12177 12177
Size: 13982
SacI G_AGCT'C
Cuts at: 4754 6064 10996 4754
Size: 1310 4932 7740
Fragments arranged by size:
7740 4932 1310
SacII CC_GC'GG
Cuts at: 9623 9623
Size: 13982
SalI G'TCGA_C
Cuts at: 11019 11019
Size: 13982
SanDI GG'GwC_CC
Cuts at: 10166 10166
Size: 13982
SapI GCTCTTCn'nnn_
Cuts at: 4532 7541 12005 12215 4532
Size: 3009 4464 210 6299
Fragments arranged by size:
6299 4464 3009 210
SbfI CC_TGCA'GG
Cuts at: 8610 10796 11029 8610
Size: 2186 233 11563
Fragments arranged by size:
11563 2186 233
ScaI AGT'ACT
Cuts at: 9117 13499 9117
Size: 4382 9600
SexAI A'CCwGG_T
Cuts at: 4107 4107
Size: 13982
SfcI C'TryA_G
Cuts at: 33 492 642 920 1022 2280 2891 3331 5324
Size: 459 150 278 102 1258 611 440 1993
Cuts at: 5324 5477 5636 5807 5897 5951 6299 6751 6818
Size: 153 159 171 90 54 348 452 67
Cuts at: 6818 7037 7412 8606 8969 9161 9312 9355 10036
Size: 219 375 1194 363 192 151 43 681
Cuts at: 10036 10792 10861 11025 11710 12703 12894 33
Size: 756 69 164 685 993 191 1121
Fragments arranged by size:
1993 1258 1194 1121 993 756 685 681
611 459 452 440 375 363 348 278
219 192 191 171 164 159 153 151
150 102 90 69 67 54 43
SfoI GGC'GCC
Cuts at: 4979 5686 6979 11414 11662 4979
Size: 707 1293 4435 248 7299
Fragments arranged by size:
7299 4435 1293 707 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 13982
SgrAI Cr'CCGG_yG
Cuts at: 5855 7299 5855
Size: 1444 12538
SmaI CCC'GGG
Cuts at: 661 5199 9078 11004 661
Size: 4538 3879 1926 3639
Fragments arranged by size:
4538 3879 3639 1926
SmlI C'TyrA_G
Cuts at: 142 503 1501 1940 2627 2875 3216 3629 5539
Size: 361 998 439 687 248 341 413 1910
Cuts at: 5539 5908 7070 7316 8597 9485 10142 10528 10768
Size: 369 1162 246 1281 888 657 386 240
Cuts at: 10768 11137 12052 12544 12806 142
Size: 369 915 492 262 1318
Fragments arranged by size:
1910 1318 1281 1162 998 915 888 687
657 492 439 413 386 369 369 361
341 262 248 246 240
SpeI A'CTAG_T
Cuts at: 67 9516 67
Size: 9449 4533
SphI G_CATG'C
Cuts at: 1197 1527 11035 11535 1197
Size: 330 9508 500 3644
Fragments arranged by size:
9508 3644 500 330
StuI AGG'CCT
Cuts at: 5841 6535 6834 7555 5841
Size: 694 299 721 12268
Fragments arranged by size:
12268 721 694 299
StyI C'CwwG_G
Cuts at: 592 1256 1977 3820 5714 5888 5935 5970 6065
Size: 664 721 1843 1894 174 47 35 95
Cuts at: 6065 6074 6878 7898 8708 8894 9094 9855 11116
Size: 9 804 1020 810 186 200 761 1261
Cuts at: 11116 11470 592
Size: 354 3104
Fragments arranged by size:
3104 1894 1843 1261 1020 810 804 761
721 664 354 200 186 174 95 47
35 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 701 951 4176 5707 6487 7383 8357 8470 8644
Size: 250 3225 1531 780 896 974 113 174
Cuts at: 8644 8770 8797 9215 9253 10096 10249 10580 10774
Size: 126 27 418 38 843 153 331 194
Cuts at: 10774 11565 11698 12157 12366 701
Size: 791 133 459 209 2317
Fragments arranged by size:
3225 2317 1531 974 896 843 791 780
459 418 331 250 209 194 174 153
133 126 113 38 27
TatI w'GTAC_w
Cuts at: 7107 7177 9115 13497 7107
Size: 70 1938 4382 7592
Fragments arranged by size:
7592 4382 1938 70
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1361 1393 1701 1733 8332 8364 8753 8785 9942
Size: 32 308 32 6599 32 389 32 1157
Cuts at: 9942 9974 10441 10473 13499 13531 1361
Size: 32 467 32 3026 32 1812
Fragments arranged by size:
6599 3026 1812 1157 467 389 308 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2704 8872 9246 10761 11779 2704
Size: 6168 374 1515 1018 4907
Fragments arranged by size:
6168 4907 1515 1018 374
XbaI T'CTAG_A
Cuts at: 733 772 805 3055 4620 4923 11013 733
Size: 39 33 2250 1565 303 6090 3702
Fragments arranged by size:
6090 3702 2250 1565 303 39 33
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 282 5781 5921 6030 6240 6373 9153 10564 11477
Size: 5499 140 109 210 133 2780 1411 913
Cuts at: 11477 282
Size: 2787
Fragments arranged by size:
5499 2787 2780 1411 913 210 140 133
109
XhoI C'TCGA_G
Cuts at: 503 1940 2627 9485 503
Size: 1437 687 6858 5000
Fragments arranged by size:
6858 5000 1437 687
XmaI C'CCGG_G
Cuts at: 659 5197 9076 11002 659
Size: 4538 3879 1926 3639
Fragments arranged by size:
4538 3879 3639 1926
XmnI GAAnn'nnTTC
Cuts at: 9332 9332
Size: 13982
ZraI GAC'GTC
Cuts at: 2182 2182
Size: 13982
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AhdI AscI BaeI BfrBI BsiWI BsrGI BssHII
BstZ17I FseI FspAI HpaI MfeI NdeI NotI NsiI
PacI PmlI PsiI SfiI SnaBI SrfI SspI SwaI