Restriction map for
pF1KA0930
(Circular) (Six-base) MAPSORT of: pF1KA0930.seq Check: 5583 from: 1 to: 4352
pF1KA0930 4352 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 919 1390 919
Size: 471 3881
Acc65I G'GTAC_C
Cuts at: 1368 1368
Size: 4352
AclI AA'CG_TT
Cuts at: 4192 4192
Size: 4352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 968 981 1246 2177 2609 2962 3624 968
Size: 13 265 931 432 353 662 1696
Fragments arranged by size:
1696 931 662 432 353 265 13
AflIII A'CryG_T
Cuts at: 502 722 1704 3504 3785 502
Size: 220 982 1800 281 1069
Fragments arranged by size:
1800 1069 982 281 220
AlwNI CAG_nnn'CTG
Cuts at: 471 1657 3095 471
Size: 1186 1438 1728
Fragments arranged by size:
1728 1438 1186
ApaI G_GGCC'C
Cuts at: 877 877
Size: 4352
ApaLI G'TGCA_C
Cuts at: 498 3190 498
Size: 2692 1660
ApoI r'AATT_y
Cuts at: 1356 3705 3961 1356
Size: 2349 256 1747
Fragments arranged by size:
2349 1747 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4352
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
AvaI C'yCGr_G
Cuts at: 1372 1372
Size: 4352
BamHI G'GATC_C
Cuts at: 1377 1377
Size: 4352
BanI G'GyrC_C
Cuts at: 1065 1368 1782 2030 2065 1065
Size: 303 414 248 35 3352
Fragments arranged by size:
3352 414 303 248 35
BanII G_rGCy'C
Cuts at: 270 751 877 1366 2396 270
Size: 481 126 489 1030 2226
Fragments arranged by size:
2226 1030 489 481 126
BbeI G_GCGC'C
Cuts at: 1786 2034 1786
Size: 248 4104
BbsI GAAGACnn'nnnn_
Cuts at: 216 216
Size: 4352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 316 350 316
Size: 34 4318
BciVI GTATCCnnnnn_n'
Cuts at: 2250 3306 4352 2250
Size: 1056 1046 2250
Fragments arranged by size:
2250 1056 1046
BclI T'GATC_A
Cuts at: 1872 1872
Size: 4352
BglI GCCn_nnn'nGGC
Cuts at: 159 811 881 901 159
Size: 652 70 20 3610
Fragments arranged by size:
3610 652 70 20
BglII A'GATC_T
Cuts at: 1867 3904 1867
Size: 2037 2315
BlpI GC'TnA_GC
Cuts at: 356 1464 356
Size: 1108 3244
Bme1580I G_kGCm'C
Cuts at: 502 877 1070 1198 1977 2070 3194 502
Size: 375 193 128 779 93 1124 1660
Fragments arranged by size:
1660 1124 779 375 193 128 93
BmrI ACTGGGnnnn_n'
Cuts at: 441 560 1564 1645 1980 4124 441
Size: 119 1004 81 335 2144 669
Fragments arranged by size:
2144 1004 669 335 119 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 4352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 942
Size: 4352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 754 1039 1528 2407 3121 3419 754
Size: 285 489 879 714 298 1687
Fragments arranged by size:
1687 879 714 489 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 410 1184 4056 410
Size: 774 2872 706
Fragments arranged by size:
2872 774 706
BsaAI yAC'GTr
Cuts at: 505 1705 505
Size: 1200 3152
BsaBI GATnn'nnATC
Cuts at: 6 1866 3903 6
Size: 1860 2037 455
Fragments arranged by size:
2037 1860 455
BsaHI Gr'CG_yC
Cuts at: 1783 2031 2733 4229 1783
Size: 248 702 1496 1906
Fragments arranged by size:
1906 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1076 1541 2062 2740 3151 3298 3572 1076
Size: 465 521 678 411 147 274 1856
Fragments arranged by size:
1856 678 521 465 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 671 701 671
Size: 30 4322
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 610 661 1135 451
Size: 159 51 474 3668
Fragments arranged by size:
3668 474 159 51
BseYI C'CCAG_C
Cuts at: 963 1777 2535 3200 3608 963
Size: 814 758 665 408 1707
Fragments arranged by size:
1707 814 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 356 1141 356
Size: 785 3567
BsiEI CG_ry'CG
Cuts at: 104 1894 1940 3170 104
Size: 1790 46 1230 1286
Fragments arranged by size:
1790 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 502 1366 2144 2334 3194 502
Size: 864 778 190 860 1660
Fragments arranged by size:
1660 864 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 270 502 751 877 1070 1198 1366 1977 2070
Size: 232 249 126 193 128 168 611 93
Cuts at: 2070 2144 2334 2396 3194 270
Size: 74 190 62 798 1428
Fragments arranged by size:
1428 798 611 249 232 193 190 168
128 126 93 74 62
BspHI T'CATG_A
Cuts at: 629 2784 629
Size: 2155 2197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1181 1402 1616 1918 2299 1181
Size: 221 214 302 381 3234
Fragments arranged by size:
3234 381 302 221 214
BsrBI CCG'CTC
Cuts at: 50 984 2644 2698 4182 4347 50
Size: 934 1660 54 1484 165 55
Fragments arranged by size:
1660 1484 934 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2264 2264
Size: 4352
BsrFI r'CCGG_y
Cuts at: 383 1028 2350 2531 383
Size: 645 1322 181 2204
Fragments arranged by size:
2204 1322 645 181
BssSI C'ACGA_G
Cuts at: 979 2623 3331 979
Size: 1644 708 2000
Fragments arranged by size:
2000 1644 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1729 1729
Size: 4352
BstBI TT'CG_AA
Cuts at: 2713 2713
Size: 4352
BstEII G'GTnAC_C
Cuts at: 483 987 483
Size: 504 3848
BstYI r'GATC_y
Cuts at: 1 1377 1844 1867 2202 2448 2754 2766 2852
Size: 1376 467 23 335 246 306 12 86
Cuts at: 2852 3904 1
Size: 1052 449
Fragments arranged by size:
1376 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 362 362
Size: 4352
BtgI C'CryG_G
Cuts at: 578 578
Size: 4352
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2302 2483 2646 2302
Size: 181 163 4008
Fragments arranged by size:
4008 181 163
BtsI GCAGTG_nn'
Cuts at: 1595 1595
Size: 4352
DraI TTT'AAA
Cuts at: 1351 1351
Size: 4352
DrdI GACnn_nn'nnGTC
Cuts at: 220 2058 3402 220
Size: 1838 1344 1170
Fragments arranged by size:
1838 1344 1170
EaeI y'GGCC_r
Cuts at: 131 160 800 1106 1937 2111 2502 2529 3874
Size: 29 640 306 831 174 391 27 1345
Cuts at: 3874 131
Size: 609
Fragments arranged by size:
1345 831 640 609 391 306 174 29
27
EagI C'GGCC_G
Cuts at: 1937 1937
Size: 4352
EarI CTCTTCn'nnn_
Cuts at: 944 2375 2585 944
Size: 1431 210 2711
Fragments arranged by size:
2711 1431 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2560 3302 3448 4162 2560
Size: 742 146 714 2750
Fragments arranged by size:
2750 742 714 146
EcoICRI GAG'CTC
Cuts at: 1364 1364
Size: 4352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 968 981 1246 2177 2609 2962 3624 942
Size: 26 13 265 931 432 353 662 1670
Fragments arranged by size:
1670 931 662 432 353 265 26 13
EcoO109I rG'GnC_Cy
Cuts at: 873 874 1217 1491 873
Size: 1 343 274 3734
Fragments arranged by size:
3734 343 274 1
EcoRI G'AATT_C
Cuts at: 1356 1356
Size: 4352
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 4352
FspI TGC'GCA
Cuts at: 865 2133 865
Size: 1268 3084
FspAI rTGC'GCAy
Cuts at: 865 865
Size: 4352
HaeII r_GCGC'y
Cuts at: 769 1786 2034 3264 4035 769
Size: 1017 248 1230 771 1086
Fragments arranged by size:
1230 1086 1017 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 671 703 2194 2224 2226 2256 671
Size: 32 1491 30 2 30 2767
Fragments arranged by size:
2767 1491 32 30 30 2
HincII GTy'rAC
Cuts at: 1391 1391
Size: 4352
KasI G'GCGC_C
Cuts at: 1782 2030 1782
Size: 248 4104
KpnI G_GTAC'C
Cuts at: 1372 1372
Size: 4352
MluI A'CGCG_T
Cuts at: 3785 3785
Size: 4352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3111 3295 3111
Size: 184 4168
MscI TGG'CCA
Cuts at: 133 2113 133
Size: 1980 2372
MslI CAynn'nnrTG
Cuts at: 1339 2341 2468 1339
Size: 1002 127 3223
Fragments arranged by size:
3223 1002 127
MspA1I CmG'CkG
Cuts at: 355 1290 1463 1777 2137 2921 3166 355
Size: 935 173 314 360 784 245 1541
Fragments arranged by size:
1541 935 784 360 314 245 173
NaeI GCC'GGC
Cuts at: 2533 2533
Size: 4352
NarI GG'CG_CC
Cuts at: 1783 2031 1783
Size: 248 4104
NcoI C'CATG_G
Cuts at: 578 578
Size: 4352
NgoMIV G'CCGG_C
Cuts at: 2531 2531
Size: 4352
NspI r_CATG'y
Cuts at: 288 1405 1905 3508 288
Size: 1117 500 1603 1132
Fragments arranged by size:
1603 1132 1117 500
PasI CC'CwG_GG
Cuts at: 879 879
Size: 4352
PciI A'CATG_T
Cuts at: 3504 3504
Size: 4352
PfoI T'CCnGG_A
Cuts at: 1130 1313 1130
Size: 183 4169
PmeI GTTT'AAAC
Cuts at: 1351 1351
Size: 4352
PmlI CAC'GTG
Cuts at: 505 505
Size: 4352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 637 669 2798 2830 637
Size: 32 2129 32 2159
Fragments arranged by size:
2159 2129 32 32
PpuMI rG'GwC_Cy
Cuts at: 1217 1217
Size: 4352
PspOMI G'GGCC_C
Cuts at: 873 873
Size: 4352
PstI C_TGCA'G
Cuts at: 198 467 1399 2084 198
Size: 269 932 685 2466
Fragments arranged by size:
2466 932 685 269
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4352
PvuII CAG'CTG
Cuts at: 355 1777 2137 355
Size: 1422 360 2570
Fragments arranged by size:
2570 1422 360
RsrII CG'GwC_CG
Cuts at: 2547 2547
Size: 4352
SacI G_AGCT'C
Cuts at: 1366 1366
Size: 4352
SalI G'TCGA_C
Cuts at: 1389 1389
Size: 4352
SapI GCTCTTCn'nnn_
Cuts at: 2375 2585 2375
Size: 210 4142
SbfI CC_TGCA'GG
Cuts at: 1399 1399
Size: 4352
ScaI AGT'ACT
Cuts at: 3869 3869
Size: 4352
SfcI C'TryA_G
Cuts at: 33 194 463 920 1395 2080 3048 3239 33
Size: 161 269 457 475 685 968 191 1146
Fragments arranged by size:
1146 968 685 475 457 269 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 159 159
Size: 4352
SfoI GGC'GCC
Cuts at: 1784 2032 1784
Size: 248 4104
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4352
SmaI CCC'GGG
Cuts at: 1374 1374
Size: 4352
SmlI C'TyrA_G
Cuts at: 769 1054 1507 2422 3136 3398 769
Size: 285 453 915 714 262 1723
Fragments arranged by size:
1723 915 714 453 285 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4352
SphI G_CATG'C
Cuts at: 1405 1905 1405
Size: 500 3852
StuI AGG'CCT
Cuts at: 410 410
Size: 4352
StyI C'CwwG_G
Cuts at: 134 578 1486 1840 134
Size: 444 908 354 2646
Fragments arranged by size:
2646 908 444 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1172 1935 2068 2527 2736 1172
Size: 763 133 459 209 2788
Fragments arranged by size:
2788 763 459 209 133
TatI w'GTAC_w
Cuts at: 842 3867 842
Size: 3025 1327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3869 3901 3869
Size: 32 4320
Tth111I GACn'n_nGTC
Cuts at: 2149 2149
Size: 4352
XbaI T'CTAG_A
Cuts at: 1383 1383
Size: 4352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 252 1847 252
Size: 1595 2757
XmaI C'CCGG_G
Cuts at: 1372 1372
Size: 4352
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BssHII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PflMI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI