Restriction map for
pF1KA0931
(Circular) (Six-base) MAPSORT of: pF1KA0931.seq Check: 4217 from: 1 to: 6881
pF1KA0931 6881 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AccI GT'mk_AC
Cuts at: 2060 3413 3919 2060
Size: 1353 506 5022
Fragments arranged by size:
5022 1353 506
Acc65I G'GTAC_C
Cuts at: 640 3897 640
Size: 3257 3624
AclI AA'CG_TT
Cuts at: 6721 6721
Size: 6881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1030 1211 1230 1822 2643 4706 5138 5491
Size: 627 181 19 592 821 2063 432 353
Cuts at: 5491 6153 403
Size: 662 1131
Fragments arranged by size:
2063 1131 821 662 627 592 432 353
181 19
AflIII A'CryG_T
Cuts at: 4233 6033 6314 4233
Size: 1800 281 4800
Fragments arranged by size:
4800 1800 281
AleI CACnn'nnGTG
Cuts at: 1435 3685 1435
Size: 2250 4631
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1326 2637 3571 1326
Size: 1311 934 4636
Fragments arranged by size:
4636 1311 934
AlwNI CAG_nnn'CTG
Cuts at: 2893 3061 4186 5624 2893
Size: 168 1125 1438 4150
Fragments arranged by size:
4150 1438 1125 168
ApaLI G'TGCA_C
Cuts at: 2946 5719 2946
Size: 2773 4108
ApoI r'AATT_y
Cuts at: 997 1229 1253 1736 3885 6234 6490 997
Size: 232 24 483 2149 2349 256 1388
Fragments arranged by size:
2349 2149 1388 483 256 232 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 6881
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6881
AvaI C'yCGr_G
Cuts at: 161 889 1822 3814 3901 161
Size: 728 933 1992 87 3141
Fragments arranged by size:
3141 1992 933 728 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2425 2458 2425
Size: 33 6848
BamHI G'GATC_C
Cuts at: 2789 3518 3906 2789
Size: 729 388 5764
Fragments arranged by size:
5764 729 388
BanI G'GyrC_C
Cuts at: 640 3897 4311 4559 4594 640
Size: 3257 414 248 35 2927
Fragments arranged by size:
3257 2927 414 248 35
BanII G_rGCy'C
Cuts at: 771 1088 2387 2524 3047 3827 3851 3895 4925
Size: 317 1299 137 523 780 24 44 1030
Cuts at: 4925 771
Size: 2727
Fragments arranged by size:
2727 1299 1030 780 523 317 137 44
24
BbeI G_GCGC'C
Cuts at: 4315 4563 4315
Size: 248 6633
BbsI GAAGACnn'nnnn_
Cuts at: 2649 3531 2649
Size: 882 5999
BbvCI CC'TCA_GC
Cuts at: 1522 1522
Size: 6881
BciVI GTATCCnnnnn_n'
Cuts at: 4779 5835 6881 4779
Size: 1056 1046 4779
Fragments arranged by size:
4779 1056 1046
BclI T'GATC_A
Cuts at: 2208 4401 2208
Size: 2193 4688
BglI GCCn_nnn'nGGC
Cuts at: 3252 3252
Size: 6881
BglII A'GATC_T
Cuts at: 1067 4396 6433 1067
Size: 3329 2037 1515
Fragments arranged by size:
3329 2037 1515
BlpI GC'TnA_GC
Cuts at: 617 759 3993 617
Size: 142 3234 3505
Fragments arranged by size:
3505 3234 142
Bme1580I G_kGCm'C
Cuts at: 2645 2950 3647 3692 4506 4599 5723 2645
Size: 305 697 45 814 93 1124 3803
Fragments arranged by size:
3803 1124 814 697 305 93 45
BmrI ACTGGGnnnn_n'
Cuts at: 1674 4093 4174 4509 6653 1674
Size: 2419 81 335 2144 1902
Fragments arranged by size:
2419 2144 1902 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5248 5248
Size: 6881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 1432 2277 2686 3306 3463 362
Size: 1070 845 409 620 157 3780
Fragments arranged by size:
3780 1070 845 620 409 157
Bpu10I CC'TnA_GC
Cuts at: 902 1522 3101 3821 902
Size: 620 1579 720 3962
Fragments arranged by size:
3962 1579 720 620
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1770 4057 4936 5650 5948 1770
Size: 2287 879 714 298 2703
Fragments arranged by size:
2703 2287 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 374 2363 6585 374
Size: 1989 4222 670
Fragments arranged by size:
4222 1989 670
BsaAI yAC'GTr
Cuts at: 1437 2606 2913 4234 1437
Size: 1169 307 1321 4084
Fragments arranged by size:
4084 1321 1169 307
BsaBI GATnn'nnATC
Cuts at: 6 4395 6432 6
Size: 4389 2037 455
Fragments arranged by size:
4389 2037 455
BsaHI Gr'CG_yC
Cuts at: 4312 4560 5262 6758 4312
Size: 248 702 1496 4435
Fragments arranged by size:
4435 1496 702 248
BsaWI w'CCGG_w
Cuts at: 4070 4591 5269 5680 5827 6101 4070
Size: 521 678 411 147 274 4850
Fragments arranged by size:
4850 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 371 401 371
Size: 30 6851
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 246 261 264 267 493 907 2395 2514 2695
Size: 15 3 3 226 414 1488 119 181
Cuts at: 2695 3871 246
Size: 1176 3256
Fragments arranged by size:
3256 1488 1176 414 226 181 119 15
3 3
BseYI C'CCAG_C
Cuts at: 447 599 851 1609 1770 2763 3214 4306 5064
Size: 152 252 758 161 993 451 1092 758
Cuts at: 5064 5729 6137 447
Size: 665 408 1191
Fragments arranged by size:
1191 1092 993 758 758 665 451 408
252 161 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1969 3009 1969
Size: 1040 5841
BsiEI CG_ry'CG
Cuts at: 104 4423 4469 5699 104
Size: 4319 46 1230 1286
Fragments arranged by size:
4319 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1088 1814 2387 2950 3895 4673 4863 5723 1088
Size: 726 573 563 945 778 190 860 2246
Fragments arranged by size:
2246 945 860 778 726 573 563 190
Bsp1286I G_dGCh'C
Cuts at: 771 1088 1814 2387 2524 2645 2950 3047 3647
Size: 317 726 573 137 121 305 97 600
Cuts at: 3647 3692 3827 3851 3895 4506 4599 4673 4863
Size: 45 135 24 44 611 93 74 190
Cuts at: 4863 4925 5723 771
Size: 62 798 1929
Fragments arranged by size:
1929 798 726 611 600 573 317 305
190 137 135 121 97 93 74 62
45 44 24
BspHI T'CATG_A
Cuts at: 1110 3759 5313 1110
Size: 2649 1554 2678
Fragments arranged by size:
2678 2649 1554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 1652 2755 3931 4145 4447 4828 652
Size: 1000 1103 1176 214 302 381 2705
Fragments arranged by size:
2705 1176 1103 1000 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 3252 5173 5227 6711 6876 50
Size: 2480 722 1921 54 1484 165 55
Fragments arranged by size:
2480 1921 1484 722 165 55 54
BsrDI GCAATG_nn'
Cuts at: 123 4793 123
Size: 4670 2211
BsrFI r'CCGG_y
Cuts at: 3809 4879 5060 3809
Size: 1070 181 5630
Fragments arranged by size:
5630 1070 181
BsrGI T'GTAC_A
Cuts at: 2770 2770
Size: 6881
BssSI C'ACGA_G
Cuts at: 876 1854 5152 5860 876
Size: 978 3298 708 1897
Fragments arranged by size:
3298 1897 978 708
BstAPI GCAn_nnn'nTGC
Cuts at: 696 4258 696
Size: 3562 3319
BstBI TT'CG_AA
Cuts at: 5242 5242
Size: 6881
BstEII G'GTnAC_C
Cuts at: 2742 2742
Size: 6881
BstXI CCAn_nnnn'nTGG
Cuts at: 3729 3729
Size: 6881
BstYI r'GATC_y
Cuts at: 1 870 1067 1440 1470 1629 1833 2048 2789
Size: 869 197 373 30 159 204 215 741
Cuts at: 2789 3438 3518 3906 4373 4396 4731 4977 5283
Size: 649 80 388 467 23 335 246 306
Cuts at: 5283 5295 5381 6433 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 869 741 649 467 449 388 373
335 306 246 215 204 197 159 86
80 30 23 12
Bsu36I CC'TnA_GG
Cuts at: 1259 1259
Size: 6881
BtgI C'CryG_G
Cuts at: 866 1394 2262 866
Size: 528 868 5485
Fragments arranged by size:
5485 868 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 834 3004 4831 5012 5175 834
Size: 2170 1827 181 163 2540
Fragments arranged by size:
2540 2170 1827 181 163
BtsI GCAGTG_nn'
Cuts at: 230 1595 2407 2555 3132 3156 3183 3191 3227
Size: 1365 812 148 577 24 27 8 36
Cuts at: 3227 3420 4124 230
Size: 193 704 2987
Fragments arranged by size:
2987 1365 812 704 577 193 148 36
27 24 8
DraI TTT'AAA
Cuts at: 3880 3880
Size: 6881
DraIII CAC_nnn'GTG
Cuts at: 1540 1540
Size: 6881
DrdI GACnn_nn'nnGTC
Cuts at: 4587 5931 4587
Size: 1344 5537
EaeI y'GGCC_r
Cuts at: 806 2508 3677 4466 4640 5031 5058 6403 806
Size: 1702 1169 789 174 391 27 1345 1284
Fragments arranged by size:
1702 1345 1284 1169 789 391 174 27
EagI C'GGCC_G
Cuts at: 4466 4466
Size: 6881
EarI CTCTTCn'nnn_
Cuts at: 264 270 285 345 422 916 1879 2423 3827
Size: 6 15 60 77 494 963 544 1404
Cuts at: 3827 4904 5114 264
Size: 1077 210 2031
Fragments arranged by size:
2031 1404 1077 963 544 494 210 77
60 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1527 2536 5089 5831 5977 6691 1527
Size: 1009 2553 742 146 714 1717
Fragments arranged by size:
2553 1717 1009 742 714 146
EcoICRI GAG'CTC
Cuts at: 1086 2385 3893 1086
Size: 1299 1508 4074
Fragments arranged by size:
4074 1508 1299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 403 1030 1211 1230 1432 1822 2277 2643
Size: 41 627 181 19 202 390 455 366
Cuts at: 2643 2686 3306 3463 4706 5138 5491 6153 362
Size: 43 620 157 1243 432 353 662 1090
Fragments arranged by size:
1243 1090 662 627 620 455 432 390
366 353 202 181 157 43 41 19
EcoNI CCTnn'n_nnAGG
Cuts at: 892 2668 892
Size: 1776 5105
EcoO109I rG'GnC_Cy
Cuts at: 1014 2673 3058 3219 4020 1014
Size: 1659 385 161 801 3875
Fragments arranged by size:
3875 1659 801 385 161
EcoRI G'AATT_C
Cuts at: 1736 3885 1736
Size: 2149 4732
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4900 4900
Size: 6881
FspI TGC'GCA
Cuts at: 469 4662 469
Size: 4193 2688
FspAI rTGC'GCAy
Cuts at: 469 469
Size: 6881
HaeII r_GCGC'y
Cuts at: 624 3258 3300 4315 4563 5793 6564 624
Size: 2634 42 1015 248 1230 771 941
Fragments arranged by size:
2634 1230 1015 941 771 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 371 403 424 456 1462 1494 1695 1727 4723
Size: 32 21 32 1006 32 201 32 2996
Cuts at: 4723 4753 4755 4785 371
Size: 30 2 30 2467
Fragments arranged by size:
2996 2467 1006 201 32 32 32 32
30 30 21 2
HincII GTy'rAC
Cuts at: 2249 2444 3920 2249
Size: 195 1476 5210
Fragments arranged by size:
5210 1476 195
HindIII A'AGCT_T
Cuts at: 1942 1942
Size: 6881
KasI G'GCGC_C
Cuts at: 4311 4559 4311
Size: 248 6633
KpnI G_GTAC'C
Cuts at: 644 3901 644
Size: 3257 3624
MfeI C'AATT_G
Cuts at: 1912 1912
Size: 6881
MluI A'CGCG_T
Cuts at: 6314 6314
Size: 6881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1598 1822 2030 5640 5824 1598
Size: 224 208 3610 184 2655
Fragments arranged by size:
3610 2655 224 208 184
MscI TGG'CCA
Cuts at: 2510 3679 4642 2510
Size: 1169 963 4749
Fragments arranged by size:
4749 1169 963
MslI CAynn'nnrTG
Cuts at: 1435 1781 3673 3685 3727 4870 4997 1435
Size: 346 1892 12 42 1143 127 3319
Fragments arranged by size:
3319 1892 1143 346 127 42 12
MspA1I CmG'CkG
Cuts at: 603 1521 2823 2932 3992 4306 4666 5450 5695
Size: 918 1302 109 1060 314 360 784 245
Cuts at: 5695 603
Size: 1789
Fragments arranged by size:
1789 1302 1060 918 784 360 314 245
109
NaeI GCC'GGC
Cuts at: 3811 5062 3811
Size: 1251 5630
NarI GG'CG_CC
Cuts at: 4312 4560 4312
Size: 248 6633
NcoI C'CATG_G
Cuts at: 1394 2262 1394
Size: 868 6013
NgoMIV G'CCGG_C
Cuts at: 3809 5060 3809
Size: 1251 5630
NspI r_CATG'y
Cuts at: 1366 2361 3499 3552 3934 4434 6037 1366
Size: 995 1138 53 382 500 1603 2210
Fragments arranged by size:
2210 1603 1138 995 500 382 53
PciI A'CATG_T
Cuts at: 6033 6033
Size: 6881
PflMI CCAn_nnn'nTGG
Cuts at: 2268 2517 2268
Size: 249 6632
PmeI GTTT'AAAC
Cuts at: 3880 3880
Size: 6881
PmlI CAC'GTG
Cuts at: 1437 2606 1437
Size: 1169 5712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5327 5359 5327
Size: 32 6849
PpuMI rG'GwC_Cy
Cuts at: 2673 3058 2673
Size: 385 6496
PspXI vC'TCGA_Gb
Cuts at: 889 1822 3814 889
Size: 933 1992 3956
Fragments arranged by size:
3956 1992 933
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 141 173 141
Size: 32 6849
PstI C_TGCA'G
Cuts at: 2039 2402 3262 3430 3928 4613 2039
Size: 363 860 168 498 685 4307
Fragments arranged by size:
4307 860 685 498 363 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6881
PvuII CAG'CTG
Cuts at: 603 1521 2932 4306 4666 603
Size: 918 1411 1374 360 2818
Fragments arranged by size:
2818 1411 1374 918 360
RsrII CG'GwC_CG
Cuts at: 3842 5076 3842
Size: 1234 5647
SacI G_AGCT'C
Cuts at: 1088 2387 3895 1088
Size: 1299 1508 4074
Fragments arranged by size:
4074 1508 1299
SalI G'TCGA_C
Cuts at: 3918 3918
Size: 6881
SapI GCTCTTCn'nnn_
Cuts at: 4904 5114 4904
Size: 210 6671
SbfI CC_TGCA'GG
Cuts at: 3928 3928
Size: 6881
ScaI AGT'ACT
Cuts at: 6398 6398
Size: 6881
SexAI A'CCwGG_T
Cuts at: 383 383
Size: 6881
SfcI C'TryA_G
Cuts at: 33 311 915 2035 2237 2398 2588 3258 3426
Size: 278 604 1120 202 161 190 670 168
Cuts at: 3426 3924 4609 5577 5768 33
Size: 498 685 968 191 1146
Fragments arranged by size:
1146 1120 968 685 670 604 498 278
202 191 190 168 161
SfoI GGC'GCC
Cuts at: 4313 4561 4313
Size: 248 6633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6881
SmaI CCC'GGG
Cuts at: 3903 3903
Size: 6881
SmlI C'TyrA_G
Cuts at: 161 889 1749 1822 3814 4036 4951 5665 5927
Size: 728 860 73 1992 222 915 714 262
Cuts at: 5927 161
Size: 1115
Fragments arranged by size:
1992 1115 915 860 728 714 262 222
73
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6881
SphI G_CATG'C
Cuts at: 3552 3934 4434 3552
Size: 382 500 5999
Fragments arranged by size:
5999 500 382
SspI AAT'ATT
Cuts at: 3009 3009
Size: 6881
StuI AGG'CCT
Cuts at: 980 1885 980
Size: 905 5976
StyI C'CwwG_G
Cuts at: 839 1223 1394 1880 2262 2785 4015 4369 839
Size: 384 171 486 382 523 1230 354 3351
Fragments arranged by size:
3351 1230 523 486 384 382 354 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1447 2387 3284 3291 3859 4464 4597 5056 5265
Size: 940 897 7 568 605 133 459 209
Cuts at: 5265 1447
Size: 3063
Fragments arranged by size:
3063 940 897 605 568 459 209 133
7
TatI w'GTAC_w
Cuts at: 2770 6396 2770
Size: 3626 3255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 862 894 1710 1742 2547 2579 2797 2829 6398
Size: 32 816 32 805 32 218 32 3569
Cuts at: 6398 6430 862
Size: 32 1313
Fragments arranged by size:
3569 1313 816 805 218 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 385 4678 385
Size: 4293 2588
XbaI T'CTAG_A
Cuts at: 3912 3912
Size: 6881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1036 4376 1036
Size: 3340 3541
XhoI C'TCGA_G
Cuts at: 161 889 1822 3814 161
Size: 728 933 1992 3228
Fragments arranged by size:
3228 1992 933 728
XmaI C'CCGG_G
Cuts at: 3901 3901
Size: 6881
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI
PpuMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AloI ApaI
AscI AvrII BcgI BfrBI BmgBI BmtI BsiWI BsmI
BsmBI BspEI BssHII BstZ17I ClaI CspCI EcoRV FseI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PshAI PsiI PspOMI SacII SanDI SfiI SgrAI
SnaBI SrfI SwaI XmnI ZraI