Restriction map for
pF1KA0933
(Circular) (Six-base) MAPSORT of: pF1KA0933.seq Check: 1462 from: 1 to: 9854
pF1KA0933 9854 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 3122 4281 4765 2381
Size: 741 1159 484 7470
Fragments arranged by size:
7470 1159 741 484
AccI GT'mk_AC
Cuts at: 2402 5114 6892 2402
Size: 2712 1778 5364
Fragments arranged by size:
5364 2712 1778
Acc65I G'GTAC_C
Cuts at: 6870 6870
Size: 9854
AclI AA'CG_TT
Cuts at: 9694 9694
Size: 9854
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 235 1210 1879 2509 3125 4300 5585 6051
Size: 89 975 669 630 616 1175 1285 466
Cuts at: 6051 7679 8111 8858 9126 146
Size: 1628 432 747 268 874
Fragments arranged by size:
1628 1285 1175 975 874 747 669 630
616 466 432 268 89
AfeI AGC'GCT
Cuts at: 3427 4156 6431 3427
Size: 729 2275 6850
Fragments arranged by size:
6850 2275 729
AflII C'TTAA_G
Cuts at: 1665 1665
Size: 9854
AflIII A'CryG_T
Cuts at: 974 1699 3326 3866 7206 8310 9287 974
Size: 725 1627 540 3340 1104 977 1541
Fragments arranged by size:
3340 1627 1541 1104 977 725 540
AgeI A'CCGG_T
Cuts at: 8242 8242
Size: 9854
AhdI GACnn_n'nnGTC
Cuts at: 1077 1752 4721 1077
Size: 675 2969 6210
Fragments arranged by size:
6210 2969 675
AleI CACnn'nnGTG
Cuts at: 3136 3136
Size: 9854
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1661 2450 4207 1661
Size: 789 1757 7308
Fragments arranged by size:
7308 1757 789
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1758 1790 2936 2968 3316 3348 1758
Size: 32 1146 32 348 32 8264
Fragments arranged by size:
8264 1146 348 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 522 1048 2118 2193 2586 3366 4296 5023 5817
Size: 526 1070 75 393 780 930 727 794
Cuts at: 5817 7159 8726 522
Size: 1342 1567 1650
Fragments arranged by size:
1650 1567 1342 1070 930 794 780 727
526 393 75
ApaI G_GGCC'C
Cuts at: 4191 4329 6511 4191
Size: 138 2182 7534
Fragments arranged by size:
7534 2182 138
ApaLI G'TGCA_C
Cuts at: 3389 5126 8624 3389
Size: 1737 3498 4619
Fragments arranged by size:
4619 3498 1737
ApoI r'AATT_y
Cuts at: 186 1059 1254 1344 1404 3724 4967 6415 6467
Size: 873 195 90 60 2320 1243 1448 52
Cuts at: 6467 6569 6637 6858 9207 9463 186
Size: 102 68 221 2349 256 577
Fragments arranged by size:
2349 2320 1448 1243 873 577 256 221
195 102 90 68 60 52
AseI AT'TA_AT
Cuts at: 20 20
Size: 9854
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 9854
AvaI C'yCGr_G
Cuts at: 198 1770 1928 2920 3082 3178 4361 6874 198
Size: 1572 158 992 162 96 1183 2513 3178
Fragments arranged by size:
3178 2513 1572 1183 992 162 158 96
AvrII C'CTAG_G
Cuts at: 4905 4905
Size: 9854
BamHI G'GATC_C
Cuts at: 111 4921 6879 111
Size: 4810 1958 3086
Fragments arranged by size:
4810 3086 1958
BanI G'GyrC_C
Cuts at: 2872 3259 6870 7284 7532 7567 2872
Size: 387 3611 414 248 35 5159
Fragments arranged by size:
5159 3611 414 387 248 35
BanII G_rGCy'C
Cuts at: 131 583 1541 1886 1907 2159 2629 3179 3560
Size: 452 958 345 21 252 470 550 381
Cuts at: 3560 4191 4329 4380 5824 6511 6868 7898 131
Size: 631 138 51 1444 687 357 1030 2087
Fragments arranged by size:
2087 1444 1030 958 687 631 550 470
452 381 357 345 252 138 51 21
BbeI G_GCGC'C
Cuts at: 3263 7288 7536 3263
Size: 4025 248 5581
Fragments arranged by size:
5581 4025 248
BbsI GAAGACnn'nnnn_
Cuts at: 702 996 1076 1548 1702 2249 2609 4040 4438
Size: 294 80 472 154 547 360 1431 398
Cuts at: 4438 5606 6057 702
Size: 1168 451 4499
Fragments arranged by size:
4499 1431 1168 547 472 451 398 360
294 154 80
BbvCI CC'TCA_GC
Cuts at: 1649 5570 1649
Size: 3921 5933
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2065 2099 2065
Size: 34 9820
BciVI GTATCCnnnnn_n'
Cuts at: 3750 5901 7752 8513 9854 3750
Size: 2151 1851 761 1341 3750
Fragments arranged by size:
3750 2151 1851 1341 761
BclI T'GATC_A
Cuts at: 4010 6081 7374 4010
Size: 2071 1293 6490
Fragments arranged by size:
6490 2071 1293
BfrBI ATG'CAT
Cuts at: 1000 1183 4500 6815 1000
Size: 183 3317 2315 4039
Fragments arranged by size:
4039 3317 2315 183
BglI GCCn_nnn'nGGC
Cuts at: 285 3157 3439 3619 285
Size: 2872 282 180 6520
Fragments arranged by size:
6520 2872 282 180
BglII A'GATC_T
Cuts at: 587 919 4244 6279 7369 9406 587
Size: 332 3325 2035 1090 2037 1035
Fragments arranged by size:
3325 2037 2035 1090 1035 332
BlpI GC'TnA_GC
Cuts at: 1714 3205 3367 5512 5810 5818 6966 1714
Size: 1491 162 2145 298 8 1148 4602
Fragments arranged by size:
4602 2145 1491 1148 298 162 8
Bme1580I G_kGCm'C
Cuts at: 3083 3393 3874 4191 4329 5130 6511 7479 7572
Size: 310 481 317 138 801 1381 968 93
Cuts at: 7572 8628 3083
Size: 1056 4309
Fragments arranged by size:
4309 1381 1056 968 801 481 317 310
138 93
BmgBI CAC'GTC
Cuts at: 1164 1700 2928 3329 3984 1164
Size: 536 1228 401 655 7034
Fragments arranged by size:
7034 1228 655 536 401
BmrI ACTGGGnnnn_n'
Cuts at: 329 527 1599 2574 5269 7066 7147 7482 9626
Size: 198 1072 975 2695 1797 81 335 2144
Cuts at: 9626 329
Size: 557
Fragments arranged by size:
2695 2144 1797 1072 975 557 335 198
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1943 3117 3546 3985 8221 1943
Size: 1174 429 439 4236 3576
Fragments arranged by size:
4236 3576 1174 439 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2518 2669 3516 3544 3715 3832 3910 4269 4664
Size: 151 847 28 171 117 78 359 395
Cuts at: 4664 5877 5893 2518
Size: 1213 16 6479
Fragments arranged by size:
6479 1213 847 395 359 171 151 117
78 28 16
Bpu10I CC'TnA_GC
Cuts at: 1649 3913 5570 1649
Size: 2264 1657 5933
Fragments arranged by size:
5933 2264 1657
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 1938 2986 4812 6000 7030 7909 8401 8699
Size: 712 1048 1826 1188 1030 879 492 298
Cuts at: 8699 1226
Size: 2381
Fragments arranged by size:
2381 1826 1188 1048 1030 879 712 492
298
BsaI GGTCTCn'nnnn_
Cuts at: 1536 2225 2413 3957 4038 4458 4884 6246 9558
Size: 689 188 1544 81 420 426 1362 3312
Cuts at: 9558 1536
Size: 1832
Fragments arranged by size:
3312 1832 1544 1362 689 426 420 188
81
BsaAI yAC'GTr
Cuts at: 3231 4416 7207 3231
Size: 1185 2791 5878
Fragments arranged by size:
5878 2791 1185
BsaBI GATnn'nnATC
Cuts at: 6 2723 7368 9405 6
Size: 2717 4645 2037 455
Fragments arranged by size:
4645 2717 2037 455
BsaHI Gr'CG_yC
Cuts at: 3260 4132 7285 7533 8235 9731 3260
Size: 872 3153 248 702 1496 3383
Fragments arranged by size:
3383 3153 1496 872 702 248
BsaWI w'CCGG_w
Cuts at: 1899 7043 7564 8242 8516 8663 9074 1899
Size: 5144 521 678 274 147 411 2679
Fragments arranged by size:
5144 2679 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 742 772 2658 2688 2803 2833 2936 2966 3316
Size: 30 1886 30 115 30 103 30 350
Cuts at: 3316 3346 3772 3802 5385 5415 5566 5596 742
Size: 30 426 30 1583 30 151 30 5000
Fragments arranged by size:
5000 1886 1583 426 350 151 115 103
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 280 904 2092 3330 3556 3787 4228 4348 4891
Size: 624 1188 1238 226 231 441 120 543
Cuts at: 4891 5587 6197 6547 280
Size: 696 610 350 3587
Fragments arranged by size:
3587 1238 1188 696 624 610 543 441
350 231 226 120
BseYI C'CCAG_C
Cuts at: 765 2263 3469 5054 5099 5791 7279 8037 8614
Size: 1498 1206 1585 45 692 1488 758 577
Cuts at: 8614 9110 765
Size: 496 1509
Fragments arranged by size:
1585 1509 1498 1488 1206 758 692 577
496 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1657 2847 4006 4438 5143 1657
Size: 1190 1159 432 705 6368
Fragments arranged by size:
6368 1190 1159 705 432
BsiEI CG_ry'CG
Cuts at: 104 747 3020 7396 7442 8650 104
Size: 643 2273 4376 46 1208 1308
Fragments arranged by size:
4376 2273 1308 1208 643 46
BsiHKAI G_wGCw'C
Cuts at: 131 1541 1625 1715 1907 1916 2629 2954 3113
Size: 1410 84 90 192 9 713 325 159
Cuts at: 3113 3281 3393 3560 3707 4259 5130 5824 6868
Size: 168 112 167 147 552 871 694 1044
Cuts at: 6868 7646 7836 8628 131
Size: 778 190 792 1357
Fragments arranged by size:
1410 1357 1044 871 792 778 713 694
552 325 192 190 168 167 159 147
112 90 84 9
BsmI GAATG_Cn'
Cuts at: 935 1474 4527 4803 6553 6817 935
Size: 539 3053 276 1750 264 3972
Fragments arranged by size:
3972 3053 1750 539 276 264
Bsp1286I G_dGCh'C
Cuts at: 131 583 1541 1625 1715 1886 1907 1916 2159
Size: 452 958 84 90 171 21 9 243
Cuts at: 2159 2629 2954 3083 3113 3179 3281 3393 3560
Size: 470 325 129 30 66 102 112 167
Cuts at: 3560 3707 3874 4191 4259 4329 4380 5130 5824
Size: 147 167 317 68 70 51 750 694
Cuts at: 5824 6511 6868 7479 7572 7646 7836 7898 8628
Size: 687 357 611 93 74 190 62 730
Cuts at: 8628 131
Size: 1357
Fragments arranged by size:
1357 958 750 730 694 687 611 470
452 357 325 317 243 190 171 167
167 147 129 112 102 93 90 84
74 70 68 66 62 51 30 21
9
BspEI T'CCGG_A
Cuts at: 9074 9074
Size: 9854
BspHI T'CATG_A
Cuts at: 2498 5955 6695 9030 2498
Size: 3457 740 2335 3322
Fragments arranged by size:
3457 3322 2335 740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2381 3122 3365 3656 3978 4281 4765 5444 6904
Size: 741 243 291 322 303 484 679 1460
Cuts at: 6904 7118 7420 7801 2381
Size: 214 302 381 4434
Fragments arranged by size:
4434 1460 741 679 484 381 322 303
302 291 243 214
BsrBI CCG'CTC
Cuts at: 50 3098 8146 8200 9684 9849 50
Size: 3048 5048 54 1484 165 55
Fragments arranged by size:
5048 3048 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2678 2702 5477 5612 7766 2678
Size: 24 2775 135 2154 4766
Fragments arranged by size:
4766 2775 2154 135 24
BsrFI r'CCGG_y
Cuts at: 474 2461 5482 7852 8033 8242 474
Size: 1987 3021 2370 181 209 2086
Fragments arranged by size:
3021 2370 2086 1987 209 181
BsrGI T'GTAC_A
Cuts at: 422 4139 5331 5633 422
Size: 3717 1192 302 4643
Fragments arranged by size:
4643 3717 1192 302
BssSI C'ACGA_G
Cuts at: 2213 4143 8125 8483 2213
Size: 1930 3982 358 3584
Fragments arranged by size:
3982 3584 1930 358
BstAPI GCAn_nnn'nTGC
Cuts at: 522 3639 4263 4416 4533 6684 7231 522
Size: 3117 624 153 117 2151 547 3145
Fragments arranged by size:
3145 3117 2151 624 547 153 117
BstBI TT'CG_AA
Cuts at: 8215 8215
Size: 9854
BstXI CCAn_nnnn'nTGG
Cuts at: 402 2559 3402 402
Size: 2157 843 6854
Fragments arranged by size:
6854 2157 843
BstYI r'GATC_y
Cuts at: 1 111 213 587 919 1107 2786 4244 4921
Size: 110 102 374 332 188 1679 1458 677
Cuts at: 4921 6279 6791 6879 7346 7369 7704 7950 8962
Size: 1358 512 88 467 23 335 246 1012
Cuts at: 8962 9048 9060 9406 1
Size: 86 12 346 449
Fragments arranged by size:
1679 1458 1358 1012 677 512 467 449
374 346 335 332 246 188 110 102
88 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 262 1616 3089 3719 5543 262
Size: 1354 1473 630 1824 4573
Fragments arranged by size:
4573 1824 1473 1354 630
BtgI C'CryG_G
Cuts at: 107 1526 3255 3401 4179 4766 4844 107
Size: 1419 1729 146 778 587 78 5117
Fragments arranged by size:
5117 1729 1419 778 587 146 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 7804 7985 8148 7804
Size: 181 163 9510
Fragments arranged by size:
9510 181 163
BtsI GCAGTG_nn'
Cuts at: 511 1826 2994 3083 5039 7097 511
Size: 1315 1168 89 1956 2058 3268
Fragments arranged by size:
3268 2058 1956 1315 1168 89
DraI TTT'AAA
Cuts at: 381 4941 6853 381
Size: 4560 1912 3382
Fragments arranged by size:
4560 3382 1912
DraIII CAC_nnn'GTG
Cuts at: 5166 5166
Size: 9854
DrdI GACnn_nn'nnGTC
Cuts at: 3988 6772 7560 8418 3988
Size: 2784 788 858 5424
Fragments arranged by size:
5424 2784 858 788
EaeI y'GGCC_r
Cuts at: 404 677 744 2543 3017 3306 3825 4040 4166
Size: 273 67 1799 474 289 519 215 126
Cuts at: 4166 6229 7439 7613 8004 8031 9376 404
Size: 2063 1210 174 391 27 1345 882
Fragments arranged by size:
2063 1799 1345 1210 882 519 474 391
289 273 215 174 126 67 27
EagI C'GGCC_G
Cuts at: 744 3017 7439 744
Size: 2273 4422 3159
Fragments arranged by size:
4422 3159 2273
EarI CTCTTCn'nnn_
Cuts at: 113 165 254 569 2086 2411 2449 3101 3209
Size: 52 89 315 1517 325 38 652 108
Cuts at: 3209 3371 4814 4932 5176 5493 6625 7877 8087
Size: 162 1443 118 244 317 1132 1252 210
Cuts at: 8087 113
Size: 1880
Fragments arranged by size:
1880 1517 1443 1252 1132 652 325 317
315 244 210 162 118 108 89 52
38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 226 682 3562 4354 5610 8062 8372 8518 9664
Size: 456 2880 792 1256 2452 310 146 1146
Cuts at: 9664 226
Size: 416
Fragments arranged by size:
2880 2452 1256 1146 792 456 416 310
146
EcoICRI GAG'CTC
Cuts at: 129 1539 1905 2627 3558 5822 6866 129
Size: 1410 366 722 931 2264 1044 3117
Fragments arranged by size:
3117 2264 1410 1044 931 722 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 146 235 1210 1879 2509 2518 2669 3125 3516
Size: 89 975 669 630 9 151 456 391
Cuts at: 3516 3544 3715 3832 3910 4269 4300 4664 5585
Size: 28 171 117 78 359 31 364 921
Cuts at: 5585 5877 5893 6051 7679 8111 8858 9126 146
Size: 292 16 158 1628 432 747 268 874
Fragments arranged by size:
1628 975 921 874 747 669 630 456
432 391 364 359 292 268 171 158
151 117 89 78 31 28 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 574 2410 2826 3004 4195 4210 4662 5167 5662
Size: 1836 416 178 1191 15 452 505 495
Cuts at: 5662 574
Size: 4766
Fragments arranged by size:
4766 1836 1191 505 495 452 416 178
15
EcoO109I rG'GnC_Cy
Cuts at: 1237 1277 2831 3564 3672 3816 3858 4187 4188
Size: 40 1554 733 108 144 42 329 1
Cuts at: 4188 5577 5868 6507 6508 6993 1237
Size: 1389 291 639 1 485 4098
Fragments arranged by size:
4098 1554 1389 733 639 485 329 291
144 108 42 40 1 1
EcoRI G'AATT_C
Cuts at: 3724 6569 6637 6858 3724
Size: 2845 68 221 6720
Fragments arranged by size:
6720 2845 221 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 176 7873 176
Size: 7697 2157
FspI TGC'GCA
Cuts at: 934 3075 7635 934
Size: 2141 4560 3153
Fragments arranged by size:
4560 3153 2141
FspAI rTGC'GCAy
Cuts at: 934 934
Size: 9854
HaeII r_GCGC'y
Cuts at: 2593 3263 3429 4158 4406 5810 6051 6433 7288
Size: 670 166 729 248 1404 241 382 855
Cuts at: 7288 7536 8558 9537 2593
Size: 248 1022 979 2910
Fragments arranged by size:
2910 1404 1022 979 855 729 670 382
248 248 241 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 205 237 275 307 911 943 1063 1095 1125
Size: 32 38 32 604 32 120 32 30
Cuts at: 1125 1157 1404 1436 1738 1770 2236 2268 2658
Size: 32 247 32 302 32 466 32 390
Cuts at: 2658 2690 2757 2789 3286 3313 3318 3345 3724
Size: 32 67 32 497 27 5 27 379
Cuts at: 3724 3748 3756 3780 4707 4739 4983 5015 5200
Size: 24 8 24 927 32 244 32 185
Cuts at: 5200 5232 6114 6146 7696 7726 7728 7758 205
Size: 32 882 32 1550 30 2 30 2301
Fragments arranged by size:
2301 1550 927 882 604 497 466 390
379 302 247 244 185 120 67 38
32 32 32 32 32 32 32 32
32 32 32 32 32 32 30 30
30 27 27 24 24 8 5 2
HincII GTy'rAC
Cuts at: 4839 5015 5115 6893 4839
Size: 176 100 1778 7800
Fragments arranged by size:
7800 1778 176 100
HindIII A'AGCT_T
Cuts at: 3459 3459
Size: 9854
HpaI GTT'AAC
Cuts at: 4839 4839
Size: 9854
KasI G'GCGC_C
Cuts at: 3259 7284 7532 3259
Size: 4025 248 5581
Fragments arranged by size:
5581 4025 248
KpnI G_GTAC'C
Cuts at: 6874 6874
Size: 9854
MluI A'CGCG_T
Cuts at: 9287 9287
Size: 9854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 617 794 3815 6120 6503 8525 8709 617
Size: 177 3021 2305 383 2022 184 1762
Fragments arranged by size:
3021 2305 2022 1762 383 184 177
MscI TGG'CCA
Cuts at: 406 679 2545 3827 4042 4168 6231 7615 406
Size: 273 1866 1282 215 126 2063 1384 2645
Fragments arranged by size:
2645 2063 1866 1384 1282 273 215 126
MslI CAynn'nnrTG
Cuts at: 2170 3136 3400 5236 7843 7970 2170
Size: 966 264 1836 2607 127 4054
Fragments arranged by size:
4054 2607 1836 966 264 127
MspA1I CmG'CkG
Cuts at: 428 1763 2046 2451 3192 3597 4029 4263 5020
Size: 1335 283 405 741 405 432 234 757
Cuts at: 5020 5507 5814 6732 6965 7279 7639 8652 8897
Size: 487 307 918 233 314 360 1013 245
Cuts at: 8897 428
Size: 1385
Fragments arranged by size:
1385 1335 1013 918 757 741 487 432
405 405 360 314 307 283 245 234
233
NaeI GCC'GGC
Cuts at: 5484 8035 5484
Size: 2551 7303
NarI GG'CG_CC
Cuts at: 3260 7285 7533 3260
Size: 4025 248 5581
Fragments arranged by size:
5581 4025 248
NcoI C'CATG_G
Cuts at: 107 3401 4766 4844 107
Size: 3294 1365 78 5117
Fragments arranged by size:
5117 3294 1365 78
NgoMIV G'CCGG_C
Cuts at: 5482 8033 5482
Size: 2551 7303
NsiI A_TGCA'T
Cuts at: 1002 1185 4502 6817 1002
Size: 183 3317 2315 4039
Fragments arranged by size:
4039 3317 2315 183
NspI r_CATG'y
Cuts at: 765 978 1690 1977 3074 4500 5379 5991 6907
Size: 213 712 287 1097 1426 879 612 916
Cuts at: 6907 7407 8314 765
Size: 500 907 2305
Fragments arranged by size:
2305 1426 1097 916 907 879 712 612
500 287 213
PasI CC'CwG_GG
Cuts at: 2892 4440 4554 2892
Size: 1548 114 8192
Fragments arranged by size:
8192 1548 114
PciI A'CATG_T
Cuts at: 974 8310 974
Size: 7336 2518
PflMI CCAn_nnn'nTGG
Cuts at: 2541 2586 2541
Size: 45 9809
PfoI T'CCnGG_A
Cuts at: 3742 3887 5681 5870 6344 3742
Size: 145 1794 189 474 7252
Fragments arranged by size:
7252 1794 474 189 145
PmeI GTTT'AAAC
Cuts at: 6853 6853
Size: 9854
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 591 623 2936 2968 3316 3348 3610 3642 8993
Size: 32 2313 32 348 32 262 32 5351
Cuts at: 8993 9025 591
Size: 32 1420
Fragments arranged by size:
5351 2313 1420 348 262 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2831 3672 3858 5577 5868 2831
Size: 841 186 1719 291 6817
Fragments arranged by size:
6817 1719 841 291 186
PshAI GACnn'nnGTC
Cuts at: 3856 3856
Size: 9854
PspOMI G'GGCC_C
Cuts at: 4187 4325 6507 4187
Size: 138 2182 7534
Fragments arranged by size:
7534 2182 138
PspXI vC'TCGA_Gb
Cuts at: 198 1770 1928 198
Size: 1572 158 8124
Fragments arranged by size:
8124 1572 158
PstI C_TGCA'G
Cuts at: 518 542 1297 2088 2660 2969 3294 3362 3653
Size: 24 755 791 572 309 325 68 291
Cuts at: 3653 4091 4268 4292 4412 4478 4776 5144 5441
Size: 438 177 24 120 66 298 368 297
Cuts at: 5441 5598 6901 7586 518
Size: 157 1303 685 2786
Fragments arranged by size:
2786 1303 791 755 685 572 438 368
325 309 298 297 291 177 157 120
68 66 24 24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 9854
PvuII CAG'CTG
Cuts at: 428 2046 2451 4029 5507 5814 6732 7279 7639
Size: 1618 405 1578 1478 307 918 547 360
Cuts at: 7639 428
Size: 2643
Fragments arranged by size:
2643 1618 1578 1478 918 547 405 360
307
RsrII CG'GwC_CG
Cuts at: 8049 8049
Size: 9854
SacI G_AGCT'C
Cuts at: 131 1541 1907 2629 3560 5824 6868 131
Size: 1410 366 722 931 2264 1044 3117
Fragments arranged by size:
3117 2264 1410 1044 931 722 366
SalI G'TCGA_C
Cuts at: 5113 6891 5113
Size: 1778 8076
SapI GCTCTTCn'nnn_
Cuts at: 113 254 2449 3101 3209 4814 5493 7877 8087
Size: 141 2195 652 108 1605 679 2384 210
Cuts at: 8087 113
Size: 1880
Fragments arranged by size:
2384 2195 1880 1605 679 652 210 141
108
SbfI CC_TGCA'GG
Cuts at: 2088 3362 5441 6901 2088
Size: 1274 2079 1460 5041
Fragments arranged by size:
5041 2079 1460 1274
ScaI AGT'ACT
Cuts at: 502 9371 502
Size: 8869 985
SexAI A'CCwGG_T
Cuts at: 5162 5162
Size: 9854
SfcI C'TryA_G
Cuts at: 33 514 538 1293 2084 2656 2965 3290 3358
Size: 481 24 755 791 572 309 325 68
Cuts at: 3358 3649 4087 4264 4288 4408 4474 4772 5140
Size: 291 438 177 24 120 66 298 368
Cuts at: 5140 5437 5594 6897 7582 8575 8766 33
Size: 297 157 1303 685 993 191 1121
Fragments arranged by size:
1303 1121 993 791 755 685 572 481
438 368 325 309 298 297 291 191
177 157 120 68 66 24 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3619 3619
Size: 9854
SfoI GGC'GCC
Cuts at: 3261 7286 7534 3261
Size: 4025 248 5581
Fragments arranged by size:
5581 4025 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 9854
SmaI CCC'GGG
Cuts at: 4363 6876 4363
Size: 2513 7341
SmlI C'TyrA_G
Cuts at: 198 1241 1665 1770 1917 1928 3001 4791 6015
Size: 1043 424 105 147 11 1073 1790 1224
Cuts at: 6015 7009 7924 8416 8678 198
Size: 994 915 492 262 1374
Fragments arranged by size:
1790 1374 1224 1073 1043 994 915 492
424 262 147 105 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 9854
SphI G_CATG'C
Cuts at: 1690 1977 3074 4500 5379 6907 7407 1690
Size: 287 1097 1426 879 1528 500 4137
Fragments arranged by size:
4137 1528 1426 1097 879 500 287
StuI AGG'CCT
Cuts at: 544 1663 2353 3622 5548 544
Size: 1119 690 1269 1926 4850
Fragments arranged by size:
4850 1926 1269 1119 690
StyI C'CwwG_G
Cuts at: 107 407 1103 1305 2790 3401 4766 4844 4905
Size: 300 696 202 1485 611 1365 78 61
Cuts at: 4905 5492 6787 6988 7342 107
Size: 587 1295 201 354 2619
Fragments arranged by size:
2619 1485 1365 1295 696 611 587 354
300 202 201 78 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 967 1348 2437 3408 4500 7437 7570 8029 8238
Size: 381 1089 971 1092 2937 133 459 209
Cuts at: 8238 967
Size: 2583
Fragments arranged by size:
2937 2583 1092 1089 971 459 381 209
133
TatI w'GTAC_w
Cuts at: 422 500 758 2040 4139 5331 5633 9369 422
Size: 78 258 1282 2099 1192 302 3736 907
Fragments arranged by size:
3736 2099 1282 1192 907 302 258 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2803 2835 6350 6382 9371 9403 2803
Size: 32 3515 32 2989 32 3254
Fragments arranged by size:
3515 3254 2989 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2052 4609 7651 2052
Size: 2557 3042 4255
Fragments arranged by size:
4255 3042 2557
XbaI T'CTAG_A
Cuts at: 4701 5553 6573 6885 4701
Size: 852 1020 312 7670
Fragments arranged by size:
7670 1020 852 312
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3483 3750 7349 3483
Size: 267 3599 5988
Fragments arranged by size:
5988 3599 267
XhoI C'TCGA_G
Cuts at: 198 1770 1928 198
Size: 1572 158 8124
Fragments arranged by size:
8124 1572 158
XmaI C'CCGG_G
Cuts at: 4361 6874 4361
Size: 2513 7341
XmnI GAAnn'nnTTC
Cuts at: 502 854 6010 6251 502
Size: 352 5156 241 4105
Fragments arranged by size:
5156 4105 352 241
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AscI BaeI BmtI BsiWI BsmBI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI MfeI NdeI NheI
NotI NruI PacI PmlI PsiI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI SwaI ZraI