Restriction map for
pF1KA0937
(Circular) (Six-base) MAPSORT of: pF1KA0937.seq Check: 8788 from: 1 to: 4970
pF1KA0937 4970 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 864 864
Size: 4970
AccI GT'mk_AC
Cuts at: 1436 2008 1436
Size: 572 4398
Acc65I G'GTAC_C
Cuts at: 1986 1986
Size: 4970
AclI AA'CG_TT
Cuts at: 4810 4810
Size: 4970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1759 2795 3227 3974 4242 877
Size: 882 1036 432 747 268 1605
Fragments arranged by size:
1605 1036 882 747 432 268
AflIII A'CryG_T
Cuts at: 2322 3426 4403 2322
Size: 1104 977 2889
Fragments arranged by size:
2889 1104 977
AgeI A'CCGG_T
Cuts at: 3358 3358
Size: 4970
AhdI GACnn_n'nnGTC
Cuts at: 288 288
Size: 4970
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 256 784 256
Size: 528 4442
AlwNI CAG_nnn'CTG
Cuts at: 811 1005 2275 3842 811
Size: 194 1270 1567 1939
Fragments arranged by size:
1939 1567 1270 194
ApaI G_GGCC'C
Cuts at: 740 1381 740
Size: 641 4329
ApaLI G'TGCA_C
Cuts at: 3740 3740
Size: 4970
ApoI r'AATT_y
Cuts at: 1974 4323 4579 1974
Size: 2349 256 2365
Fragments arranged by size:
2365 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4970
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
AvaI C'yCGr_G
Cuts at: 1022 1682 1990 1022
Size: 660 308 4002
Fragments arranged by size:
4002 660 308
BamHI G'GATC_C
Cuts at: 1115 1995 1115
Size: 880 4090
BanI G'GyrC_C
Cuts at: 572 838 1313 1534 1786 1986 2400 2648 2683
Size: 266 475 221 252 200 414 248 35
Cuts at: 2683 572
Size: 2859
Fragments arranged by size:
2859 475 414 266 252 248 221 200
35
BanII G_rGCy'C
Cuts at: 740 925 1381 1984 3014 740
Size: 185 456 603 1030 2696
Fragments arranged by size:
2696 1030 603 456 185
BbeI G_GCGC'C
Cuts at: 576 2404 2652 576
Size: 1828 248 2894
Fragments arranged by size:
2894 1828 248
BbsI GAAGACnn'nnnn_
Cuts at: 865 1534 865
Size: 669 4301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4936
BciVI GTATCCnnnnn_n'
Cuts at: 2868 3629 4970 2868
Size: 761 1341 2868
Fragments arranged by size:
2868 1341 761
BclI T'GATC_A
Cuts at: 2490 2490
Size: 4970
BglII A'GATC_T
Cuts at: 2485 4522 2485
Size: 2037 2933
BlpI GC'TnA_GC
Cuts at: 2082 2082
Size: 4970
Bme1580I G_kGCm'C
Cuts at: 740 1381 2595 2688 3744 740
Size: 641 1214 93 1056 1966
Fragments arranged by size:
1966 1214 1056 641 93
BmgBI CAC'GTC
Cuts at: 1434 1434
Size: 4970
BmrI ACTGGGnnnn_n'
Cuts at: 325 678 743 917 1011 1051 1121 2182 2263
Size: 353 65 174 94 40 70 1061 81
Cuts at: 2263 2598 4742 325
Size: 335 2144 553
Fragments arranged by size:
2144 1061 553 353 335 174 94 81
70 65 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1872 3337 1872
Size: 1465 3505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 879 1529 1776 800
Size: 79 650 247 3994
Fragments arranged by size:
3994 650 247 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2146 3025 3517 3815 2146
Size: 879 492 298 3301
Fragments arranged by size:
3301 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4674 4674
Size: 4970
BsaAI yAC'GTr
Cuts at: 507 2323 507
Size: 1816 3154
BsaBI GATnn'nnATC
Cuts at: 6 2484 4521 6
Size: 2478 2037 455
Fragments arranged by size:
2478 2037 455
BsaHI Gr'CG_yC
Cuts at: 165 415 573 2401 2649 3351 4847 165
Size: 250 158 1828 248 702 1496 288
Fragments arranged by size:
1828 1496 702 288 250 248 158
BsaWI w'CCGG_w
Cuts at: 881 958 1610 1647 1710 2159 2680 3358 3632
Size: 77 652 37 63 449 521 678 274
Cuts at: 3632 3779 4190 881
Size: 147 411 1661
Fragments arranged by size:
1661 678 652 521 449 411 274 147
77 63 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 391 421 1527 1557 340
Size: 30 21 30 1106 30 3753
Fragments arranged by size:
3753 1106 30 30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 348 348
Size: 4970
BseYI C'CCAG_C
Cuts at: 177 243 548 2395 3153 3730 4226 177
Size: 66 305 1847 758 577 496 921
Fragments arranged by size:
1847 921 758 577 496 305 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1148 1317 1148
Size: 169 4801
BsiEI CG_ry'CG
Cuts at: 104 125 159 2512 2558 3766 104
Size: 21 34 2353 46 1208 1308
Fragments arranged by size:
2353 1308 1208 46 34 21
BsiHKAI G_wGCw'C
Cuts at: 155 1984 2762 2952 3744 155
Size: 1829 778 190 792 1381
Fragments arranged by size:
1829 1381 792 778 190
BsmI GAATG_Cn'
Cuts at: 1637 1637
Size: 4970
BsmBI CGTCTCn'nnnn_
Cuts at: 268 268
Size: 4970
Bsp1286I G_dGCh'C
Cuts at: 155 740 925 1381 1984 2595 2688 2762 2952
Size: 585 185 456 603 611 93 74 190
Cuts at: 2952 3014 3744 155
Size: 62 730 1381
Fragments arranged by size:
1381 730 611 603 585 456 190 185
93 74 62
BspEI T'CCGG_A
Cuts at: 881 1610 1710 4190 881
Size: 729 100 2480 1661
Fragments arranged by size:
2480 1661 729 100
BspHI T'CATG_A
Cuts at: 1121 4146 1121
Size: 3025 1945
BspMI ACCTGCnnnn'nnnn_
Cuts at: 293 864 1004 1513 2020 2234 2536 2917 293
Size: 571 140 509 507 214 302 381 2346
Fragments arranged by size:
2346 571 509 507 381 302 214 140
BsrBI CCG'CTC
Cuts at: 50 3262 3316 4800 4965 50
Size: 3212 54 1484 165 55
Fragments arranged by size:
3212 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1010 2882 1010
Size: 1872 3098
BsrFI r'CCGG_y
Cuts at: 214 569 2968 3149 3358 214
Size: 355 2399 181 209 1826
Fragments arranged by size:
2399 1826 355 209 181
BsrGI T'GTAC_A
Cuts at: 1463 1463
Size: 4970
BssSI C'ACGA_G
Cuts at: 1750 3241 3599 1750
Size: 1491 358 3121
Fragments arranged by size:
3121 1491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2347 2347
Size: 4970
BstBI TT'CG_AA
Cuts at: 3331 3331
Size: 4970
BstXI CCAn_nnnn'nTGG
Cuts at: 114 825 1344 1896 114
Size: 711 519 552 3188
Fragments arranged by size:
3188 711 552 519
BstYI r'GATC_y
Cuts at: 1 1115 1995 2462 2485 2820 3066 4078 4164
Size: 1114 880 467 23 335 246 1012 86
Cuts at: 4164 4176 4522 1
Size: 12 346 449
Fragments arranged by size:
1114 1012 880 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 614 1365 614
Size: 751 4219
BtgI C'CryG_G
Cuts at: 125 524 593 716 917 1872 125
Size: 399 69 123 201 955 3223
Fragments arranged by size:
3223 955 399 201 123 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 854 2920 3101 3264 854
Size: 2066 181 163 2560
Fragments arranged by size:
2560 2066 181 163
BtsI GCAGTG_nn'
Cuts at: 799 1501 1946 2213 799
Size: 702 445 267 3556
Fragments arranged by size:
3556 702 445 267
DraI TTT'AAA
Cuts at: 1969 1969
Size: 4970
DrdI GACnn_nn'nnGTC
Cuts at: 2676 3534 2676
Size: 858 4112
EaeI y'GGCC_r
Cuts at: 122 156 628 974 1007 1428 1587 1869 2555
Size: 34 472 346 33 421 159 282 686
Cuts at: 2555 2729 3120 3147 4492 122
Size: 174 391 27 1345 600
Fragments arranged by size:
1345 686 600 472 421 391 346 282
174 159 34 33 27
EagI C'GGCC_G
Cuts at: 122 156 2555 122
Size: 34 2399 2537
Fragments arranged by size:
2537 2399 34
EarI CTCTTCn'nnn_
Cuts at: 1663 2993 3203 1663
Size: 1330 210 3430
Fragments arranged by size:
3430 1330 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 295 316 556 3178 3488 3634 4780 295
Size: 21 240 2622 310 146 1146 485
Fragments arranged by size:
2622 1146 485 310 240 146 21
EcoICRI GAG'CTC
Cuts at: 1982 1982
Size: 4970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 800 877 879 1529 1759 1776 2795 3227 3974
Size: 77 2 650 230 17 1019 432 747
Cuts at: 3974 4242 800
Size: 268 1528
Fragments arranged by size:
1528 1019 747 650 432 268 230 77
17 2
EcoO109I rG'GnC_Cy
Cuts at: 605 737 943 1044 1377 2109 605
Size: 132 206 101 333 732 3466
Fragments arranged by size:
3466 732 333 206 132 101
EcoRI G'AATT_C
Cuts at: 1974 1974
Size: 4970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2989 2989
Size: 4970
FspI TGC'GCA
Cuts at: 2751 2751
Size: 4970
HaeII r_GCGC'y
Cuts at: 555 576 1682 2404 2652 3674 4653 555
Size: 21 1106 722 248 1022 979 872
Fragments arranged by size:
1106 1022 979 872 722 248 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 343 375 391 423 525 557 2812
Size: 32 37 32 16 32 102 32 2255
Cuts at: 2812 2842 2844 2874 274
Size: 30 2 30 2370
Fragments arranged by size:
2370 2255 102 37 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 1089 2009 1089
Size: 920 4050
KasI G'GCGC_C
Cuts at: 572 2400 2648 572
Size: 1828 248 2894
Fragments arranged by size:
2894 1828 248
KpnI G_GTAC'C
Cuts at: 1990 1990
Size: 4970
MluI A'CGCG_T
Cuts at: 4403 4403
Size: 4970
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1250 1525 3641 3825 1250
Size: 275 2116 184 2395
Fragments arranged by size:
2395 2116 275 184
MscI TGG'CCA
Cuts at: 630 976 1009 1589 1871 2731 630
Size: 346 33 580 282 860 2869
Fragments arranged by size:
2869 860 580 346 282 33
MslI CAynn'nnrTG
Cuts at: 430 455 1342 2959 3086 430
Size: 25 887 1617 127 2314
Fragments arranged by size:
2314 1617 887 127 25
MspA1I CmG'CkG
Cuts at: 161 181 747 1132 2081 2395 2755 3768 4013
Size: 20 566 385 949 314 360 1013 245
Cuts at: 4013 161
Size: 1118
Fragments arranged by size:
1118 1013 949 566 385 360 314 245
20
NaeI GCC'GGC
Cuts at: 216 571 3151 216
Size: 355 2580 2035
Fragments arranged by size:
2580 2035 355
NarI GG'CG_CC
Cuts at: 573 2401 2649 573
Size: 1828 248 2894
Fragments arranged by size:
2894 1828 248
NcoI C'CATG_G
Cuts at: 524 593 917 1872 524
Size: 69 324 955 3622
Fragments arranged by size:
3622 955 324 69
NgoMIV G'CCGG_C
Cuts at: 214 569 3149 214
Size: 355 2580 2035
Fragments arranged by size:
2580 2035 355
NspI r_CATG'y
Cuts at: 454 771 903 2023 2523 3430 454
Size: 317 132 1120 500 907 1994
Fragments arranged by size:
1994 1120 907 500 317 132
PasI CC'CwG_GG
Cuts at: 367 640 1216 1926 367
Size: 273 576 710 3411
Fragments arranged by size:
3411 710 576 273
PciI A'CATG_T
Cuts at: 3426 3426
Size: 4970
PflMI CCAn_nnn'nTGG
Cuts at: 811 811
Size: 4970
PfoI T'CCnGG_A
Cuts at: 409 777 409
Size: 368 4602
PmeI GTTT'AAAC
Cuts at: 1969 1969
Size: 4970
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 4109
Size: 32 4938
PpuMI rG'GwC_Cy
Cuts at: 605 943 1044 605
Size: 338 101 4531
Fragments arranged by size:
4531 338 101
PshAI GACnn'nnGTC
Cuts at: 260 260
Size: 4970
PspOMI G'GGCC_C
Cuts at: 736 1377 736
Size: 641 4329
PstI C_TGCA'G
Cuts at: 290 1001 2017 2702 290
Size: 711 1016 685 2558
Fragments arranged by size:
2558 1016 711 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4970
PvuII CAG'CTG
Cuts at: 747 2395 2755 747
Size: 1648 360 2962
Fragments arranged by size:
2962 1648 360
RsrII CG'GwC_CG
Cuts at: 3165 3165
Size: 4970
SacI G_AGCT'C
Cuts at: 1984 1984
Size: 4970
SalI G'TCGA_C
Cuts at: 2007 2007
Size: 4970
SanDI GG'GwC_CC
Cuts at: 1044 1044
Size: 4970
SapI GCTCTTCn'nnn_
Cuts at: 2993 3203 2993
Size: 210 4760
SbfI CC_TGCA'GG
Cuts at: 2017 2017
Size: 4970
ScaI AGT'ACT
Cuts at: 4487 4487
Size: 4970
SexAI A'CCwGG_T
Cuts at: 1403 1403
Size: 4970
SfcI C'TryA_G
Cuts at: 33 171 286 742 997 1300 1620 2013 2698
Size: 138 115 456 255 303 320 393 685
Cuts at: 2698 3691 3882 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 456 393 320 303 255
191 138 115
SfoI GGC'GCC
Cuts at: 574 2402 2650 574
Size: 1828 248 2894
Fragments arranged by size:
2894 1828 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4970
SgrAI Cr'CCGG_yG
Cuts at: 569 569
Size: 4970
SmaI CCC'GGG
Cuts at: 1024 1992 1024
Size: 968 4002
SmlI C'TyrA_G
Cuts at: 2125 3040 3532 3794 2125
Size: 915 492 262 3301
Fragments arranged by size:
3301 915 492 262
SnaBI TAC'GTA
Cuts at: 507 507
Size: 4970
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4970
SphI G_CATG'C
Cuts at: 454 903 2023 2523 454
Size: 449 1120 500 2901
Fragments arranged by size:
2901 1120 500 449
StyI C'CwwG_G
Cuts at: 524 593 917 1097 1872 2104 2458 524
Size: 69 324 180 775 232 354 3036
Fragments arranged by size:
3036 775 354 324 232 180 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1193 1331 1552 1607 2553 2686 3145 3354 1193
Size: 138 221 55 946 133 459 209 2809
Fragments arranged by size:
2809 946 459 221 209 138 133 55
TatI w'GTAC_w
Cuts at: 1463 4485 1463
Size: 3022 1948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1527 1559 4487 4519 1527
Size: 32 2928 32 1978
Fragments arranged by size:
2928 1978 32 32
Tth111I GACn'n_nGTC
Cuts at: 1816 2767 1816
Size: 951 4019
XbaI T'CTAG_A
Cuts at: 2001 2001
Size: 4970
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1582 2465 1582
Size: 883 4087
XmaI C'CCGG_G
Cuts at: 1022 1990 1022
Size: 968 4002
XmnI GAAnn'nnTTC
Cuts at: 301 1672 301
Size: 1371 3599
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI Bpu10I BsiWI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SfiI SrfI
SspI StuI SwaI XhoI ZraI