Restriction map for
pF1KA0938
(Circular) (Six-base) MAPSORT of: pF1KA0938.seq Check: 2143 from: 1 to: 8639
pF1KA0938 8639 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 8639
AatII G_ACGT'C
Cuts at: 4356 4356
Size: 8639
AccI GT'mk_AC
Cuts at: 813 949 5677 813
Size: 136 4728 3775
Fragments arranged by size:
4728 3775 136
Acc65I G'GTAC_C
Cuts at: 907 5655 907
Size: 4748 3891
AclI AA'CG_TT
Cuts at: 8479 8479
Size: 8639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 491 542 844 1535 1706 2905 3272 3496
Size: 334 51 302 691 171 1199 367 224
Cuts at: 3496 3821 3907 5293 5398 6464 6896 7249 7911
Size: 325 86 1386 105 1066 432 353 662
Cuts at: 7911 157
Size: 885
Fragments arranged by size:
1386 1199 1066 885 691 662 432 367
353 334 325 302 224 171 105 86
51
AflII C'TTAA_G
Cuts at: 2580 2580
Size: 8639
AflIII A'CryG_T
Cuts at: 482 992 1037 5991 7791 8072 482
Size: 510 45 4954 1800 281 1049
Fragments arranged by size:
4954 1800 1049 510 281 45
AgeI A'CCGG_T
Cuts at: 4028 4028
Size: 8639
AhdI GACnn_n'nnGTC
Cuts at: 4758 4758
Size: 8639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 134 134
Size: 8639
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1306 1338 1306
Size: 32 8607
AlwNI CAG_nnn'CTG
Cuts at: 342 515 2288 2802 3862 4045 4159 5944 7382
Size: 173 1773 514 1060 183 114 1785 1438
Cuts at: 7382 342
Size: 1599
Fragments arranged by size:
1785 1773 1599 1438 1060 514 183 173
114
ApaI G_GGCC'C
Cuts at: 3047 3047
Size: 8639
ApaLI G'TGCA_C
Cuts at: 1631 2294 7477 1631
Size: 663 5183 2793
Fragments arranged by size:
5183 2793 663
ApoI r'AATT_y
Cuts at: 690 1892 3018 3486 5643 7992 8248 690
Size: 1202 1126 468 2157 2349 256 1081
Fragments arranged by size:
2349 2157 1202 1126 1081 468 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8639
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8639
AvaI C'yCGr_G
Cuts at: 936 1193 1522 1645 2172 3040 5556 5659 936
Size: 257 329 123 527 868 2516 103 3916
Fragments arranged by size:
3916 2516 868 527 329 257 123 103
BamHI G'GATC_C
Cuts at: 2317 4297 5664 2317
Size: 1980 1367 5292
Fragments arranged by size:
5292 1980 1367
BanI G'GyrC_C
Cuts at: 907 1602 2365 5655 6069 6317 6352 907
Size: 695 763 3290 414 248 35 3194
Fragments arranged by size:
3290 3194 763 695 414 248 35
BanII G_rGCy'C
Cuts at: 2725 3013 3047 3172 3899 4411 4835 4876 5380
Size: 288 34 125 727 512 424 41 504
Cuts at: 5380 5404 5653 6683 2725
Size: 24 249 1030 4681
Fragments arranged by size:
4681 1030 727 512 504 424 288 249
125 41 34 24
BbeI G_GCGC'C
Cuts at: 1606 6073 6321 1606
Size: 4467 248 3924
Fragments arranged by size:
4467 3924 248
BbsI GAAGACnn'nnnn_
Cuts at: 413 641 706 1034 1498 1523 3567 5140 5299
Size: 228 65 328 464 25 2044 1573 159
Cuts at: 5299 5606 413
Size: 307 3446
Fragments arranged by size:
3446 2044 1573 464 328 307 228 159
65 25
BbvCI CC'TCA_GC
Cuts at: 1378 2746 3863 3943 1378
Size: 1368 1117 80 6074
Fragments arranged by size:
6074 1368 1117 80
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 8605
BciVI GTATCCnnnnn_n'
Cuts at: 2336 2676 4483 6537 7593 8639 2336
Size: 340 1807 2054 1056 1046 2336
Fragments arranged by size:
2336 2054 1807 1056 1046 340
BclI T'GATC_A
Cuts at: 1841 3963 6159 1841
Size: 2122 2196 4321
Fragments arranged by size:
4321 2196 2122
BglI GCCn_nnn'nGGC
Cuts at: 1611 2066 2756 3502 4068 1611
Size: 455 690 746 566 6182
Fragments arranged by size:
6182 746 690 566 455
BglII A'GATC_T
Cuts at: 1714 2827 3546 6154 8191 1714
Size: 1113 719 2608 2037 2162
Fragments arranged by size:
2608 2162 2037 1113 719
BlpI GC'TnA_GC
Cuts at: 360 2518 3807 4058 5379 5751 360
Size: 2158 1289 251 1321 372 3248
Fragments arranged by size:
3248 2158 1321 1289 372 251
Bme1580I G_kGCm'C
Cuts at: 1635 2298 3047 6264 6357 7481 1635
Size: 663 749 3217 93 1124 2793
Fragments arranged by size:
3217 2793 1124 749 663 93
BmgBI CAC'GTC
Cuts at: 5138 5138
Size: 8639
BmrI ACTGGGnnnn_n'
Cuts at: 1894 4831 5851 5932 6267 8411 1894
Size: 2937 1020 81 335 2144 2122
Fragments arranged by size:
2937 2144 2122 1020 335 81
BmtI G_CTAG'C
Cuts at: 4427 4427
Size: 8639
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 321 1416 2849 4849 7006 321
Size: 1095 1433 2000 2157 1954
Fragments arranged by size:
2157 2000 1954 1433 1095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 766 852 1593 2491 2583 2933 3157 3955 5308
Size: 86 741 898 92 350 224 798 1353
Cuts at: 5308 766
Size: 4097
Fragments arranged by size:
4097 1353 898 798 741 350 224 92
86
Bpu10I CC'TnA_GC
Cuts at: 1378 2746 3381 3863 3943 1378
Size: 1368 635 482 80 6074
Fragments arranged by size:
6074 1368 635 482 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 702 4071 5815 6694 7408 7706 702
Size: 3369 1744 879 714 298 1635
Fragments arranged by size:
3369 1744 1635 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 219 1279 1408 2308 2641 8343 219
Size: 1060 129 900 333 5702 515
Fragments arranged by size:
5702 1060 900 515 333 129
BsaAI yAC'GTr
Cuts at: 5992 5992
Size: 8639
BsaBI GATnn'nnATC
Cuts at: 6 6153 8190 6
Size: 6147 2037 455
Fragments arranged by size:
6147 2037 455
BsaHI Gr'CG_yC
Cuts at: 1603 4353 6070 6318 7020 8516 1603
Size: 2750 1717 248 702 1496 1726
Fragments arranged by size:
2750 1726 1717 1496 702 248
BsaWI w'CCGG_w
Cuts at: 137 2485 3986 4028 5828 6349 7027 7438 7585
Size: 2348 1501 42 1800 521 678 411 147
Cuts at: 7585 7859 137
Size: 274 917
Fragments arranged by size:
2348 1800 1501 917 678 521 411 274
147 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 472 502 2464 2494 3702 3732 472
Size: 30 1962 30 1208 30 5379
Fragments arranged by size:
5379 1962 1208 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 237 541 586 2078 4089 237
Size: 304 45 1492 2011 4787
Fragments arranged by size:
4787 2011 1492 304 45
BseYI C'CCAG_C
Cuts at: 382 515 591 984 1244 2119 2434 2604 3046
Size: 133 76 393 260 875 315 170 442
Cuts at: 3046 3072 3110 5403 6064 6822 7487 7895 382
Size: 26 38 2293 661 758 665 408 1126
Fragments arranged by size:
2293 1126 875 758 665 661 442 408
393 315 260 170 133 76 38 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 2378 4827 5433 355
Size: 2023 2449 606 3561
Fragments arranged by size:
3561 2449 2023 606
BsiEI CG_ry'CG
Cuts at: 104 6181 6227 7457 104
Size: 6077 46 1230 1286
Fragments arranged by size:
6077 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1635 2298 2725 3519 3899 4411 4835 5380 5563
Size: 663 427 794 380 512 424 545 183
Cuts at: 5563 5653 6431 6621 7481 1635
Size: 90 778 190 860 2793
Fragments arranged by size:
2793 860 794 778 663 545 512 427
424 380 190 183 90
BsmI GAATG_Cn'
Cuts at: 2051 3567 3856 2051
Size: 1516 289 6834
Fragments arranged by size:
6834 1516 289
BsmBI CGTCTCn'nnnn_
Cuts at: 4360 4360
Size: 8639
Bsp1286I G_dGCh'C
Cuts at: 1635 2298 2725 3013 3047 3172 3519 3899 4411
Size: 663 427 288 34 125 347 380 512
Cuts at: 4411 4835 4876 5380 5404 5563 5653 6264 6357
Size: 424 41 504 24 159 90 611 93
Cuts at: 6357 6431 6621 6683 7481 1635
Size: 74 190 62 798 2793
Fragments arranged by size:
2793 798 663 611 512 504 427 424
380 347 288 190 159 125 93 90
74 62 41 34 24
BspEI T'CCGG_A
Cuts at: 137 137
Size: 8639
BspHI T'CATG_A
Cuts at: 2807 7071 2807
Size: 4264 4375
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2365 5689 5903 6205 6586 2365
Size: 3324 214 302 381 4418
Fragments arranged by size:
4418 3324 381 302 214
BsrBI CCG'CTC
Cuts at: 50 300 6931 6985 8469 8634 50
Size: 250 6631 54 1484 165 55
Fragments arranged by size:
6631 1484 250 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4418 6551 4418
Size: 2133 6506
BsrFI r'CCGG_y
Cuts at: 4028 6637 6818 4028
Size: 2609 181 5849
Fragments arranged by size:
5849 2609 181
BssSI C'ACGA_G
Cuts at: 2638 2718 2788 3149 6910 7618 2638
Size: 80 70 361 3761 708 3659
Fragments arranged by size:
3761 3659 708 361 80 70
BstAPI GCAn_nnn'nTGC
Cuts at: 6016 6016
Size: 8639
BstBI TT'CG_AA
Cuts at: 2352 5055 7000 2352
Size: 2703 1945 3991
Fragments arranged by size:
3991 2703 1945
BstXI CCAn_nnnn'nTGG
Cuts at: 3118 3135 3793 3118
Size: 17 658 7964
Fragments arranged by size:
7964 658 17
BstYI r'GATC_y
Cuts at: 1 1714 2074 2317 2827 3546 3968 4297 4450
Size: 1713 360 243 510 719 422 329 153
Cuts at: 4450 5253 5340 5508 5664 6131 6154 6489 6735
Size: 803 87 168 156 467 23 335 246
Cuts at: 6735 7041 7053 7139 8191 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1713 1052 803 719 510 467 449 422
360 335 329 306 246 243 168 156
153 87 86 23 12
BstZ17I GTA'TAC
Cuts at: 950 950
Size: 8639
Bsu36I CC'TnA_GG
Cuts at: 1430 1767 5393 1430
Size: 337 3626 4676
Fragments arranged by size:
4676 3626 337
BtgI C'CryG_G
Cuts at: 230 1467 5219 5332 5371 230
Size: 1237 3752 113 39 3498
Fragments arranged by size:
3752 3498 1237 113 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6589 6770 6933 6589
Size: 181 163 8295
Fragments arranged by size:
8295 181 163
BtsI GCAGTG_nn'
Cuts at: 909 1032 1275 1746 1857 2511 3630 4227 4815
Size: 123 243 471 111 654 1119 597 588
Cuts at: 4815 5300 5882 909
Size: 485 582 3666
Fragments arranged by size:
3666 1119 654 597 588 582 485 471
243 123 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2358 2393 4380 4415 2358
Size: 35 1987 35 6582
Fragments arranged by size:
6582 1987 35 35
DraI TTT'AAA
Cuts at: 5638 5638
Size: 8639
DraIII CAC_nnn'GTG
Cuts at: 1224 1224
Size: 8639
DrdI GACnn_nn'nnGTC
Cuts at: 6345 7689 6345
Size: 1344 7295
EaeI y'GGCC_r
Cuts at: 2058 3396 4069 4215 6224 6398 6789 6816 8161
Size: 1338 673 146 2009 174 391 27 1345
Cuts at: 8161 2058
Size: 2536
Fragments arranged by size:
2536 2009 1345 1338 673 391 174 146
27
EagI C'GGCC_G
Cuts at: 6224 6224
Size: 8639
EarI CTCTTCn'nnn_
Cuts at: 426 518 692 1572 2545 2873 3090 3210 3737
Size: 92 174 880 973 328 217 120 527
Cuts at: 3737 3883 4107 6662 6872 426
Size: 146 224 2555 210 2193
Fragments arranged by size:
2555 2193 973 880 527 328 224 217
210 174 146 120 92
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6847 7589 7735 8449 6847
Size: 742 146 714 7037
Fragments arranged by size:
7037 742 714 146
EcoICRI GAG'CTC
Cuts at: 2723 3897 4409 4833 5378 5651 2723
Size: 1174 512 424 545 273 5711
Fragments arranged by size:
5711 1174 545 512 424 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 491 542 766 844 852 1535 1593 1706
Size: 334 51 224 78 8 683 58 113
Cuts at: 1706 2491 2583 2905 2933 3157 3272 3496 3821
Size: 785 92 322 28 224 115 224 325
Cuts at: 3821 3907 3955 5293 5308 5398 6464 6896 7249
Size: 86 48 1338 15 90 1066 432 353
Cuts at: 7249 7911 157
Size: 662 885
Fragments arranged by size:
1338 1066 885 785 683 662 432 353
334 325 322 224 224 224 115 113
92 90 86 78 58 51 48 28
15 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2060 2060
Size: 8639
EcoO109I rG'GnC_Cy
Cuts at: 3939 5778 3939
Size: 1839 6800
EcoRI G'AATT_C
Cuts at: 3486 5643 3486
Size: 2157 6482
EcoRV GAT'ATC
Cuts at: 1083 4887 5050 1083
Size: 3804 163 4672
Fragments arranged by size:
4672 3804 163
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1736 2261 6658 1736
Size: 525 4397 3717
Fragments arranged by size:
4397 3717 525
FspI TGC'GCA
Cuts at: 238 6420 238
Size: 6182 2457
FspAI rTGC'GCAy
Cuts at: 238 238
Size: 8639
HaeII r_GCGC'y
Cuts at: 306 1606 2172 2288 6073 6321 7551 8322 306
Size: 1300 566 116 3785 248 1230 771 623
Fragments arranged by size:
3785 1300 1230 771 623 566 248 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 451 483 546 578 691 723 862
Size: 32 13 32 63 32 113 32 139
Cuts at: 862 894 959 991 1257 1289 2323 2355 2464
Size: 32 65 32 266 32 1034 32 109
Cuts at: 2464 2496 2819 2851 3625 3657 3790 3822 4261
Size: 32 323 32 774 32 133 32 439
Cuts at: 4261 4293 4608 4640 4744 4776 5127 5159 5289
Size: 32 315 32 104 32 351 32 130
Cuts at: 5289 5321 6481 6511 6513 6543 406
Size: 32 1160 30 2 30 2502
Fragments arranged by size:
2502 1160 1034 774 439 351 323 315
266 139 133 130 113 109 104 65
63 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 30 30 13 2
HincII GTy'rAC
Cuts at: 814 2190 5678 814
Size: 1376 3488 3775
Fragments arranged by size:
3775 3488 1376
HindIII A'AGCT_T
Cuts at: 1541 1667 2577 1541
Size: 126 910 7603
Fragments arranged by size:
7603 910 126
KasI G'GCGC_C
Cuts at: 1602 6069 6317 1602
Size: 4467 248 3924
Fragments arranged by size:
4467 3924 248
KpnI G_GTAC'C
Cuts at: 911 5659 911
Size: 4748 3891
MfeI C'AATT_G
Cuts at: 4733 4905 4733
Size: 172 8467
MluI A'CGCG_T
Cuts at: 8072 8072
Size: 8639
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 269 1367 2123 4479 7398 7582 269
Size: 1098 756 2356 2919 184 1326
Fragments arranged by size:
2919 2356 1326 1098 756 184
MscI TGG'CCA
Cuts at: 6400 6400
Size: 8639
MslI CAynn'nnrTG
Cuts at: 566 823 1472 3469 4211 4519 5370 6628 6755
Size: 257 649 1997 742 308 851 1258 127
Cuts at: 6755 566
Size: 2450
Fragments arranged by size:
2450 1997 1258 851 742 649 308 257
127
MspA1I CmG'CkG
Cuts at: 114 1244 1377 2980 3250 3424 3885 5418 5750
Size: 1130 133 1603 270 174 461 1533 332
Cuts at: 5750 6064 6424 7208 7453 114
Size: 314 360 784 245 1300
Fragments arranged by size:
1603 1533 1300 1130 784 461 360 332
314 270 245 174 133
NaeI GCC'GGC
Cuts at: 6820 6820
Size: 8639
NarI GG'CG_CC
Cuts at: 1603 6070 6318 1603
Size: 4467 248 3924
Fragments arranged by size:
4467 3924 248
NcoI C'CATG_G
Cuts at: 1467 5219 5332 5371 1467
Size: 3752 113 39 4735
Fragments arranged by size:
4735 3752 113 39
NgoMIV G'CCGG_C
Cuts at: 6818 6818
Size: 8639
NheI G'CTAG_C
Cuts at: 4423 4423
Size: 8639
NspI r_CATG'y
Cuts at: 851 996 1041 1587 1987 5692 6192 7795 851
Size: 145 45 546 400 3705 500 1603 1695
Fragments arranged by size:
3705 1695 1603 546 500 400 145 45
PasI CC'CwG_GG
Cuts at: 882 882
Size: 8639
PciI A'CATG_T
Cuts at: 992 1037 7791 992
Size: 45 6754 1840
Fragments arranged by size:
6754 1840 45
PflMI CCAn_nnn'nTGG
Cuts at: 830 830
Size: 8639
PfoI T'CCnGG_A
Cuts at: 1570 3971 1570
Size: 2401 6238
PmeI GTTT'AAAC
Cuts at: 5638 5638
Size: 8639
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7085 7117 7085
Size: 32 8607
PsiI TTA'TAA
Cuts at: 2575 2575
Size: 8639
PspOMI G'GGCC_C
Cuts at: 3043 3043
Size: 8639
PspXI vC'TCGA_Gb
Cuts at: 936 2172 5556 936
Size: 1236 3384 4019
Fragments arranged by size:
4019 3384 1236
PstI C_TGCA'G
Cuts at: 2673 2979 3884 5686 6371 2673
Size: 306 905 1802 685 4941
Fragments arranged by size:
4941 1802 905 685 306
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8639
PvuII CAG'CTG
Cuts at: 1244 1377 2980 3250 3885 5418 6064 6424 1244
Size: 133 1603 270 635 1533 646 360 3459
Fragments arranged by size:
3459 1603 1533 646 635 360 270 133
RsrII CG'GwC_CG
Cuts at: 6834 6834
Size: 8639
SacI G_AGCT'C
Cuts at: 2725 3899 4411 4835 5380 5653 2725
Size: 1174 512 424 545 273 5711
Fragments arranged by size:
5711 1174 545 512 424 273
SalI G'TCGA_C
Cuts at: 812 5676 812
Size: 4864 3775
SapI GCTCTTCn'nnn_
Cuts at: 3210 3737 3883 6662 6872 3210
Size: 527 146 2779 210 4977
Fragments arranged by size:
4977 2779 527 210 146
SbfI CC_TGCA'GG
Cuts at: 5686 5686
Size: 8639
ScaI AGT'ACT
Cuts at: 8156 8156
Size: 8639
SfcI C'TryA_G
Cuts at: 33 2564 2669 2975 3245 3880 5682 6367 7335
Size: 2531 105 306 270 635 1802 685 968
Cuts at: 7335 7526 33
Size: 191 1146
Fragments arranged by size:
2531 1802 1146 968 685 635 306 270
191 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 2066 2066
Size: 8639
SfoI GGC'GCC
Cuts at: 1604 6071 6319 1604
Size: 4467 248 3924
Fragments arranged by size:
4467 3924 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8639
SmaI CCC'GGG
Cuts at: 1195 1647 3042 5661 1195
Size: 452 1395 2619 4173
Fragments arranged by size:
4173 2619 1395 452
SmlI C'TyrA_G
Cuts at: 681 936 2172 2580 4086 5556 5794 6709 7423
Size: 255 1236 408 1506 1470 238 915 714
Cuts at: 7423 7685 681
Size: 262 1635
Fragments arranged by size:
1635 1506 1470 1236 915 714 408 262
255 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8639
SphI G_CATG'C
Cuts at: 5692 6192 5692
Size: 500 8139
StyI C'CwwG_G
Cuts at: 266 1086 1467 1593 2369 4398 5219 5332 5371
Size: 820 381 126 776 2029 821 113 39
Cuts at: 5371 5773 6127 266
Size: 402 354 2778
Fragments arranged by size:
2778 2029 821 820 776 402 381 354
126 113 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 583 874 1048 2358 4662 5343 6222 6355 6814
Size: 291 174 1310 2304 681 879 133 459
Cuts at: 6814 7023 583
Size: 209 2199
Fragments arranged by size:
2304 2199 1310 879 681 459 291 209
174 133
TatI w'GTAC_w
Cuts at: 8154 8154
Size: 8639
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3702 3734 8156 8188 3702
Size: 32 4422 32 4153
Fragments arranged by size:
4422 4153 32 32
Tth111I GACn'n_nGTC
Cuts at: 705 1497 5107 6436 705
Size: 792 3610 1329 2908
Fragments arranged by size:
3610 2908 1329 792
XbaI T'CTAG_A
Cuts at: 600 4974 5236 5670 600
Size: 4374 262 434 3569
Fragments arranged by size:
4374 3569 434 262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1474 3616 4746 6134 1474
Size: 2142 1130 1388 3979
Fragments arranged by size:
3979 2142 1388 1130
XhoI C'TCGA_G
Cuts at: 936 2172 5556 936
Size: 1236 3384 4019
Fragments arranged by size:
4019 3384 1236
XmaI C'CCGG_G
Cuts at: 1193 1645 3040 5659 1193
Size: 452 1395 2619 4173
Fragments arranged by size:
4173 2619 1395 452
XmnI GAAnn'nnTTC
Cuts at: 3194 3194
Size: 8639
ZraI GAC'GTC
Cuts at: 4354 4354
Size: 8639
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PciI PflMI PfoI PmeI PpiI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AleI AscI AvrII BaeI BfrBI BsiWI BsrGI
BssHII BstEII ClaI FseI HpaI NdeI NotI NruI
NsiI PacI PmlI PpuMI PshAI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI