Restriction map for
pF1KA0942
(Circular) (Six-base) MAPSORT of: pF1KA0942.seq Check: 812 from: 1 to: 4652
pF1KA0942 4652 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 1394 1394
Size: 4652
AccI GT'mk_AC
Cuts at: 1690 1690
Size: 4652
Acc65I G'GTAC_C
Cuts at: 235 1668 235
Size: 1433 3219
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 979 1037 1321 1342 1540 2477 2909 3262
Size: 588 58 284 21 198 937 432 353
Cuts at: 3262 3924 391
Size: 662 1119
Fragments arranged by size:
1119 937 662 588 432 353 284 198
58 21
AflIII A'CryG_T
Cuts at: 1094 1589 2004 3804 4085 1094
Size: 495 415 1800 281 1661
Fragments arranged by size:
1800 1661 495 415 281
AhdI GACnn_n'nnGTC
Cuts at: 797 797
Size: 4652
AlwNI CAG_nnn'CTG
Cuts at: 1957 3395 1957
Size: 1438 3214
ApaLI G'TGCA_C
Cuts at: 1045 3490 1045
Size: 2445 2207
ApoI r'AATT_y
Cuts at: 197 340 887 1656 4005 4261 197
Size: 143 547 769 2349 256 588
Fragments arranged by size:
2349 769 588 547 256 143
AseI AT'TA_AT
Cuts at: 20 20
Size: 4652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 1533 1672 1533
Size: 139 4513
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4652
BanI G'GyrC_C
Cuts at: 235 1668 2082 2330 2365 235
Size: 1433 414 248 35 2522
Fragments arranged by size:
2522 1433 414 248 35
BanII G_rGCy'C
Cuts at: 115 1150 1666 2696 115
Size: 1035 516 1030 2071
Fragments arranged by size:
2071 1035 1030 516
BbeI G_GCGC'C
Cuts at: 2086 2334 2086
Size: 248 4404
BbsI GAAGACnn'nnnn_
Cuts at: 919 919
Size: 4652
BciVI GTATCCnnnnn_n'
Cuts at: 1548 2550 3606 4652 1548
Size: 1002 1056 1046 1548
Fragments arranged by size:
1548 1056 1046 1002
BclI T'GATC_A
Cuts at: 2172 2172
Size: 4652
BglII A'GATC_T
Cuts at: 2167 4204 2167
Size: 2037 2615
BlpI GC'TnA_GC
Cuts at: 1764 1764
Size: 4652
Bme1580I G_kGCm'C
Cuts at: 1049 2277 2370 3494 1049
Size: 1228 93 1124 2207
Fragments arranged by size:
2207 1228 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1864 1945 2280 4424 1864
Size: 81 335 2144 2092
Fragments arranged by size:
2144 2092 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1371 3019 1371
Size: 1648 3004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1140 1444 1140
Size: 304 4348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 1450 1828 2707 3421 3719 437
Size: 1013 378 879 714 298 1370
Fragments arranged by size:
1370 1013 879 714 378 298
BsaI GGTCTCn'nnnn_
Cuts at: 205 4356 205
Size: 4151 501
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 2166 4203 6
Size: 2160 2037 455
Fragments arranged by size:
2160 2037 455
BsaHI Gr'CG_yC
Cuts at: 1391 2083 2331 3033 4529 1391
Size: 692 248 702 1496 1514
Fragments arranged by size:
1514 1496 702 692 248
BsaWI w'CCGG_w
Cuts at: 1841 2362 3040 3451 3598 3872 1841
Size: 521 678 411 147 274 2621
Fragments arranged by size:
2621 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 778 1306 778
Size: 528 4124
BseYI C'CCAG_C
Cuts at: 145 2077 2835 3500 3908 145
Size: 1932 758 665 408 889
Fragments arranged by size:
1932 889 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1190
Size: 4652
BsiEI CG_ry'CG
Cuts at: 104 2194 2240 3470 104
Size: 2090 46 1230 1286
Fragments arranged by size:
2090 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 193 1049 1352 1666 2444 2634 3494 193
Size: 856 303 314 778 190 860 1351
Fragments arranged by size:
1351 860 856 778 314 303 190
BsmI GAATG_Cn'
Cuts at: 430 1460 430
Size: 1030 3622
Bsp1286I G_dGCh'C
Cuts at: 115 193 1049 1150 1352 1666 2277 2370 2444
Size: 78 856 101 202 314 611 93 74
Cuts at: 2444 2634 2696 3494 115
Size: 190 62 798 1273
Fragments arranged by size:
1273 856 798 611 314 202 190 101
93 78 74 62
BspHI T'CATG_A
Cuts at: 840 3084 840
Size: 2244 2408
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1702 1916 2218 2599 1702
Size: 214 302 381 3755
Fragments arranged by size:
3755 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1355 2944 2998 4482 4647 50
Size: 1305 1589 54 1484 165 55
Fragments arranged by size:
1589 1484 1305 165 55 54
BsrDI GCAATG_nn'
Cuts at: 147 252 515 537 620 2564 147
Size: 105 263 22 83 1944 2235
Fragments arranged by size:
2235 1944 263 105 83 22
BsrFI r'CCGG_y
Cuts at: 2650 2831 2650
Size: 181 4471
BsrGI T'GTAC_A
Cuts at: 683 860 683
Size: 177 4475
BssSI C'ACGA_G
Cuts at: 2923 3631 2923
Size: 708 3944
BstAPI GCAn_nnn'nTGC
Cuts at: 2029 2029
Size: 4652
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 267 592 1341 267
Size: 325 749 3578
Fragments arranged by size:
3578 749 325
BstYI r'GATC_y
Cuts at: 1 666 1677 2144 2167 2502 2748 3054 3066
Size: 665 1011 467 23 335 246 306 12
Cuts at: 3066 3152 4204 1
Size: 86 1052 449
Fragments arranged by size:
1052 1011 665 467 449 335 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1070 107
Size: 963 3689
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2602 2783 2946 2602
Size: 181 163 4308
Fragments arranged by size:
4308 181 163
BtsI GCAGTG_nn'
Cuts at: 1568 1895 1568
Size: 327 4325
DraI TTT'AAA
Cuts at: 426 1651 426
Size: 1225 3427
DrdI GACnn_nn'nnGTC
Cuts at: 1123 1375 2358 3702 1123
Size: 252 983 1344 2073
Fragments arranged by size:
2073 1344 983 252
EaeI y'GGCC_r
Cuts at: 269 582 1343 2237 2411 2802 2829 4174 269
Size: 313 761 894 174 391 27 1345 747
Fragments arranged by size:
1345 894 761 747 391 313 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4652
EarI CTCTTCn'nnn_
Cuts at: 120 725 1013 2675 2885 120
Size: 605 288 1662 210 1887
Fragments arranged by size:
1887 1662 605 288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2860 3602 3748 4462 2860
Size: 742 146 714 3050
Fragments arranged by size:
3050 742 714 146
EcoICRI GAG'CTC
Cuts at: 1664 1664
Size: 4652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 391 979 1037 1140 1321 1342 1444 1540 2477
Size: 588 58 103 181 21 102 96 937
Cuts at: 2477 2909 3262 3924 391
Size: 432 353 662 1119
Fragments arranged by size:
1119 937 662 588 432 353 181 103
102 96 58 21
EcoO109I rG'GnC_Cy
Cuts at: 1791 1791
Size: 4652
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4652
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4652
HaeII r_GCGC'y
Cuts at: 2086 2334 3564 4335 2086
Size: 248 1230 771 2403
Fragments arranged by size:
2403 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 387 419 594 626 783 815 1590 1622 2494
Size: 32 175 32 157 32 775 32 872
Cuts at: 2494 2524 2526 2556 387
Size: 30 2 30 2483
Fragments arranged by size:
2483 872 775 175 157 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4652
HindIII A'AGCT_T
Cuts at: 706 706
Size: 4652
KasI G'GCGC_C
Cuts at: 2082 2330 2082
Size: 248 4404
KpnI G_GTAC'C
Cuts at: 239 1672 239
Size: 1433 3219
MfeI C'AATT_G
Cuts at: 1182 1182
Size: 4652
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3411 3595 3411
Size: 184 4468
MscI TGG'CCA
Cuts at: 271 584 2413 271
Size: 313 1829 2510
Fragments arranged by size:
2510 1829 313
MslI CAynn'nnrTG
Cuts at: 2641 2768 2641
Size: 127 4525
MspA1I CmG'CkG
Cuts at: 288 1195 1763 2077 2437 3221 3466 288
Size: 907 568 314 360 784 245 1474
Fragments arranged by size:
1474 907 784 568 360 314 245
NaeI GCC'GGC
Cuts at: 2833 2833
Size: 4652
NarI GG'CG_CC
Cuts at: 2083 2331 2083
Size: 248 4404
NcoI C'CATG_G
Cuts at: 107 107
Size: 4652
NgoMIV G'CCGG_C
Cuts at: 2831 2831
Size: 4652
NspI r_CATG'y
Cuts at: 1705 2205 3808 1705
Size: 500 1603 2549
Fragments arranged by size:
2549 1603 500
PciI A'CATG_T
Cuts at: 3804 3804
Size: 4652
PflMI CCAn_nnn'nTGG
Cuts at: 1056 1422 1056
Size: 366 4286
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 3098
Size: 32 4620
PspXI vC'TCGA_Gb
Cuts at: 1533 1533
Size: 4652
PstI C_TGCA'G
Cuts at: 1515 1699 2384 1515
Size: 184 685 3783
Fragments arranged by size:
3783 685 184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 288 1195 2077 2437 288
Size: 907 882 360 2503
Fragments arranged by size:
2503 907 882 360
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4652
SacI G_AGCT'C
Cuts at: 1666 1666
Size: 4652
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 120 2675 2885 120
Size: 2555 210 1887
Fragments arranged by size:
2555 1887 210
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4652
ScaI AGT'ACT
Cuts at: 759 813 4169 759
Size: 54 3356 1242
Fragments arranged by size:
3356 1242 54
SfcI C'TryA_G
Cuts at: 33 1511 1695 2380 3348 3539 33
Size: 1478 184 685 968 191 1146
Fragments arranged by size:
1478 1146 968 685 191 184
SfoI GGC'GCC
Cuts at: 2084 2332 2084
Size: 248 4404
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 1674 1674
Size: 4652
SmlI C'TyrA_G
Cuts at: 452 1465 1533 1807 2722 3436 3698 452
Size: 1013 68 274 915 714 262 1406
Fragments arranged by size:
1406 1013 915 714 274 262 68
SpeI A'CTAG_T
Cuts at: 67 351 67
Size: 284 4368
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
SspI AAT'ATT
Cuts at: 591 591
Size: 4652
StuI AGG'CCT
Cuts at: 1009 1009
Size: 4652
StyI C'CwwG_G
Cuts at: 107 324 662 1064 1385 1786 2140 107
Size: 217 338 402 321 401 354 2619
Fragments arranged by size:
2619 402 401 354 338 321 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2235 2368 2827 3036 2235
Size: 133 459 209 3851
Fragments arranged by size:
3851 459 209 133
TatI w'GTAC_w
Cuts at: 683 757 811 860 954 1400 4167 683
Size: 74 54 49 94 446 2767 1168
Fragments arranged by size:
2767 1168 446 94 74 54 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4620
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 246 2147 246
Size: 1901 2751
XhoI C'TCGA_G
Cuts at: 1533 1533
Size: 4652
XmaI C'CCGG_G
Cuts at: 1672 1672
Size: 4652
ZraI GAC'GTC
Cuts at: 1392 1392
Size: 4652
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsiWI BsmBI BspEI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XmnI