Restriction map for
pF1KA0943
(Circular) (Six-base) MAPSORT of: pF1KA0943.seq Check: 4396 from: 1 to: 4292
pF1KA0943 4292 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AatII G_ACGT'C
Cuts at: 1199 1199
Size: 4292
AccI GT'mk_AC
Cuts at: 1330 1330
Size: 4292
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 583 856 1103 2117 2549 2902 3564 417
Size: 166 273 247 1014 432 353 662 1145
Fragments arranged by size:
1145 1014 662 432 353 273 247 166
AflIII A'CryG_T
Cuts at: 1644 3444 3725 1644
Size: 1800 281 2211
Fragments arranged by size:
2211 1800 281
AleI CACnn'nnGTG
Cuts at: 628 628
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 561 864 1597 3035 561
Size: 303 733 1438 1818
Fragments arranged by size:
1818 1438 733 303
ApaI G_GGCC'C
Cuts at: 542 542
Size: 4292
ApaLI G'TGCA_C
Cuts at: 3130 3130
Size: 4292
ApoI r'AATT_y
Cuts at: 1296 3645 3901 1296
Size: 2349 256 1687
Fragments arranged by size:
2349 1687 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 1312 1312
Size: 4292
AvrII C'CTAG_G
Cuts at: 381 381
Size: 4292
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 377 679 1103 1138 1308 1722 1970 2005 377
Size: 302 424 35 170 414 248 35 2664
Fragments arranged by size:
2664 424 414 302 248 170 35 35
BanII G_rGCy'C
Cuts at: 182 542 595 803 929 971 1306 2336 182
Size: 360 53 208 126 42 335 1030 2138
Fragments arranged by size:
2138 1030 360 335 208 126 53 42
BbeI G_GCGC'C
Cuts at: 683 1726 1974 683
Size: 1043 248 3001
Fragments arranged by size:
3001 1043 248
BbsI GAAGACnn'nnnn_
Cuts at: 570 1271 570
Size: 701 3591
BbvCI CC'TCA_GC
Cuts at: 737 737
Size: 4292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1017 1051 1017
Size: 34 4258
BciVI GTATCCnnnnn_n'
Cuts at: 182 2190 3246 4292 182
Size: 2008 1056 1046 182
Fragments arranged by size:
2008 1056 1046 182
BclI T'GATC_A
Cuts at: 1812 1812
Size: 4292
BglII A'GATC_T
Cuts at: 230 1807 3844 230
Size: 1577 2037 678
Fragments arranged by size:
2037 1577 678
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 542 1143 1917 2010 3134 542
Size: 601 774 93 1124 1700
Fragments arranged by size:
1700 1124 774 601 93
BmrI ACTGGGnnnn_n'
Cuts at: 202 852 1504 1585 1920 4064 202
Size: 650 652 81 335 2144 430
Fragments arranged by size:
2144 652 650 430 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2659 2659
Size: 4292
Bpu10I CC'TnA_GC
Cuts at: 737 737
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 3061 3359 1468
Size: 879 714 298 2401
Fragments arranged by size:
2401 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 163 759 3996 163
Size: 596 3237 459
Fragments arranged by size:
3237 596 459
BsaAI yAC'GTr
Cuts at: 587 825 1645 587
Size: 238 820 3234
Fragments arranged by size:
3234 820 238
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 680 869 1196 1723 1971 2673 4169 680
Size: 189 327 527 248 702 1496 803
Fragments arranged by size:
1496 803 702 527 327 248 189
BsaWI w'CCGG_w
Cuts at: 140 1481 2002 2680 3091 3238 3512 140
Size: 1341 521 678 411 147 274 920
Fragments arranged by size:
1341 920 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 725 755 725
Size: 30 4262
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 937 937
Size: 4292
BseYI C'CCAG_C
Cuts at: 320 1717 2475 3140 3548 320
Size: 1397 758 665 408 1064
Fragments arranged by size:
1397 1064 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 675 695 675
Size: 20 4272
BsiEI CG_ry'CG
Cuts at: 104 708 1834 1880 3110 104
Size: 604 1126 46 1230 1286
Fragments arranged by size:
1286 1230 1126 604 46
BsiHKAI G_wGCw'C
Cuts at: 595 929 1306 2084 2274 3134 595
Size: 334 377 778 190 860 1753
Fragments arranged by size:
1753 860 778 377 334 190
BsmI GAATG_Cn'
Cuts at: 448 448
Size: 4292
BsmBI CGTCTCn'nnnn_
Cuts at: 823 823
Size: 4292
Bsp1286I G_dGCh'C
Cuts at: 182 542 595 803 929 971 1143 1306 1917
Size: 360 53 208 126 42 172 163 611
Cuts at: 1917 2010 2084 2274 2336 3134 182
Size: 93 74 190 62 798 1340
Fragments arranged by size:
1340 798 611 360 208 190 172 163
126 93 74 62 53 42
BspHI T'CATG_A
Cuts at: 848 2724 848
Size: 1876 2416
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1342 1556 1858 2239 1342
Size: 214 302 381 3395
Fragments arranged by size:
3395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 608 620 2204 608
Size: 12 1584 2696
Fragments arranged by size:
2696 1584 12
BsrFI r'CCGG_y
Cuts at: 374 707 2290 2471 374
Size: 333 1583 181 2195
Fragments arranged by size:
2195 1583 333 181
BssSI C'ACGA_G
Cuts at: 2563 3271 2563
Size: 708 3584
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 2653 2653
Size: 4292
BstEII G'GTnAC_C
Cuts at: 741 741
Size: 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 1062 1062
Size: 4292
BstYI r'GATC_y
Cuts at: 1 230 1317 1784 1807 2142 2388 2694 2706
Size: 229 1087 467 23 335 246 306 12
Cuts at: 2706 2792 3844 1
Size: 86 1052 449
Fragments arranged by size:
1087 1052 467 449 335 306 246 229
86 23 12
BtgI C'CryG_G
Cuts at: 107 760 962 107
Size: 653 202 3437
Fragments arranged by size:
3437 653 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1020 1041 2242 2423 2586 1020
Size: 21 1201 181 163 2726
Fragments arranged by size:
2726 1201 181 163 21
BtsI GCAGTG_nn'
Cuts at: 683 711 840 1005 1535 683
Size: 28 129 165 530 3440
Fragments arranged by size:
3440 530 165 129 28
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1998 3342 1998
Size: 1344 2948
EaeI y'GGCC_r
Cuts at: 1877 2051 2442 2469 3814 1877
Size: 174 391 27 1345 2355
Fragments arranged by size:
2355 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 406 2315 2525 406
Size: 1909 210 2173
Fragments arranged by size:
2173 1909 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2500 3242 3388 4102 2500
Size: 742 146 714 2690
Fragments arranged by size:
2690 742 714 146
EcoICRI GAG'CTC
Cuts at: 593 927 1304 593
Size: 334 377 3581
Fragments arranged by size:
3581 377 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 417 583 856 1103 2117 2549 2902 3564 417
Size: 166 273 247 1014 432 353 662 1145
Fragments arranged by size:
1145 1014 662 432 353 273 247 166
EcoO109I rG'GnC_Cy
Cuts at: 301 558 1431 301
Size: 257 873 3162
Fragments arranged by size:
3162 873 257
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 861 2311 861
Size: 1450 2842
FspI TGC'GCA
Cuts at: 2073 2073
Size: 4292
HaeII r_GCGC'y
Cuts at: 683 896 1726 1974 3204 3975 683
Size: 213 830 248 1230 771 1000
Fragments arranged by size:
1230 1000 830 771 248 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 232 252 264 335 367 725 757 2134
Size: 12 20 12 71 32 358 32 1377
Cuts at: 2134 2164 2166 2196 220
Size: 30 2 30 2316
Fragments arranged by size:
2316 1377 358 71 32 32 30 30
20 12 12 2
HincII GTy'rAC
Cuts at: 1331 1331
Size: 4292
HindIII A'AGCT_T
Cuts at: 568 568
Size: 4292
KasI G'GCGC_C
Cuts at: 679 1722 1970 679
Size: 1043 248 3001
Fragments arranged by size:
3001 1043 248
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 728 3051 3235 728
Size: 2323 184 1785
Fragments arranged by size:
2323 1785 184
MscI TGG'CCA
Cuts at: 2053 2053
Size: 4292
MslI CAynn'nnrTG
Cuts at: 613 628 1060 2281 2408 613
Size: 15 432 1221 127 2497
Fragments arranged by size:
2497 1221 432 127 15
MspA1I CmG'CkG
Cuts at: 1403 1717 2077 2861 3106 1403
Size: 314 360 784 245 2589
Fragments arranged by size:
2589 784 360 314 245
NaeI GCC'GGC
Cuts at: 376 2473 376
Size: 2097 2195
NarI GG'CG_CC
Cuts at: 680 1723 1971 680
Size: 1043 248 3001
Fragments arranged by size:
3001 1043 248
NcoI C'CATG_G
Cuts at: 107 760 107
Size: 653 3639
NgoMIV G'CCGG_C
Cuts at: 374 2471 374
Size: 2097 2195
NspI r_CATG'y
Cuts at: 333 1345 1845 3448 333
Size: 1012 500 1603 1177
Fragments arranged by size:
1603 1177 1012 500
PasI CC'CwG_GG
Cuts at: 864 864
Size: 4292
PciI A'CATG_T
Cuts at: 3444 3444
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 289 366 864 289
Size: 77 498 3717
Fragments arranged by size:
3717 498 77
PfoI T'CCnGG_A
Cuts at: 989 989
Size: 4292
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2738 2770 2738
Size: 32 4260
PpuMI rG'GwC_Cy
Cuts at: 558 558
Size: 4292
PspOMI G'GGCC_C
Cuts at: 538 538
Size: 4292
PstI C_TGCA'G
Cuts at: 699 1339 2024 699
Size: 640 685 2967
Fragments arranged by size:
2967 685 640
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 1717 2077 1717
Size: 360 3932
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 595 929 1306 595
Size: 334 377 3581
Fragments arranged by size:
3581 377 334
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 695 1335 2020 2988 3179 33
Size: 662 640 685 968 191 1146
Fragments arranged by size:
1146 968 685 662 640 191
SfoI GGC'GCC
Cuts at: 681 1724 1972 681
Size: 1043 248 3001
Fragments arranged by size:
3001 1043 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 1314 1314
Size: 4292
SmlI C'TyrA_G
Cuts at: 1447 2362 3076 3338 1447
Size: 915 714 262 2401
Fragments arranged by size:
2401 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 333 1345 1845 333
Size: 1012 500 2780
Fragments arranged by size:
2780 1012 500
StuI AGG'CCT
Cuts at: 820 820
Size: 4292
StyI C'CwwG_G
Cuts at: 107 381 596 760 1426 1780 107
Size: 274 215 164 666 354 2619
Fragments arranged by size:
2619 666 354 274 215 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1875 2008 2467 2676 1875
Size: 133 459 209 3491
Fragments arranged by size:
3491 459 209 133
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 675 707 3809 3841 675
Size: 32 3102 32 1126
Fragments arranged by size:
3102 1126 32 32
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 250 1323 250
Size: 1073 3219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1155 1787 1155
Size: 632 3660
XmaI C'CCGG_G
Cuts at: 1312 1312
Size: 4292
XmnI GAAnn'nnTTC
Cuts at: 161 161
Size: 4292
ZraI GAC'GTC
Cuts at: 1197 1197
Size: 4292
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AgeI AhdI AlfI AloI AscI
BaeI BfrBI BglI BmgBI BmtI BpmI BsiWI BspEI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI