Restriction map for
pF1KA0944
(Circular) (Six-base) MAPSORT of: pF1KA0944.seq Check: 5398 from: 1 to: 15185
pF1KA0944 15185 bp
With 182 enzymes: SgfI *
June 20, 2008 10:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4315 8107 11311 4315
Size: 3792 3204 8189
Fragments arranged by size:
8189 3792 3204
AatII G_ACGT'C
Cuts at: 1403 1403
Size: 15185
AccI GT'mk_AC
Cuts at: 6478 11557 12223 6478
Size: 5079 666 9440
Fragments arranged by size:
9440 5079 666
Acc65I G'GTAC_C
Cuts at: 2258 4470 8962 11423 12201 2258
Size: 2212 4492 2461 778 5242
Fragments arranged by size:
5242 4492 2461 2212 778
AclI AA'CG_TT
Cuts at: 372 15025 372
Size: 14653 532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2182 2227 2351 3010 4459 4672 5132 7117 7643
Size: 45 124 659 1449 213 460 1985 526
Cuts at: 7643 8588 9856 10235 10552 11515 13010 13442 14189
Size: 945 1268 379 317 963 1495 432 747
Cuts at: 14189 14457 2182
Size: 268 2910
Fragments arranged by size:
2910 1985 1495 1449 1268 963 945 747
659 526 460 432 379 317 268 213
124 45
AflII C'TTAA_G
Cuts at: 7257 8620 7257
Size: 1363 13822
AflIII A'CryG_T
Cuts at: 666 3192 3742 4375 5372 6827 7484 7703 9292
Size: 2526 550 633 997 1455 657 219 1589
Cuts at: 9292 10964 12537 13641 14618 666
Size: 1672 1573 1104 977 1233
Fragments arranged by size:
2526 1672 1589 1573 1455 1233 1104 997
977 657 633 550 219
AgeI A'CCGG_T
Cuts at: 11764 13573 11764
Size: 1809 13376
AhdI GACnn_n'nnGTC
Cuts at: 3105 3105
Size: 15185
AleI CACnn'nnGTG
Cuts at: 3379 3775 6712 3379
Size: 396 2937 11852
Fragments arranged by size:
11852 2937 396
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 530 2342 4877 7528 530
Size: 1812 2535 2651 8187
Fragments arranged by size:
8187 2651 2535 1812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 15153
AlwNI CAG_nnn'CTG
Cuts at: 1285 2666 4330 6046 6788 9450 10382 11326 11971
Size: 1381 1664 1716 742 2662 932 944 645
Cuts at: 11971 12490 14057 1285
Size: 519 1567 2413
Fragments arranged by size:
2662 2413 1716 1664 1567 1381 944 932
742 645 519
ApaI G_GGCC'C
Cuts at: 8320 10514 8320
Size: 2194 12991
ApaLI G'TGCA_C
Cuts at: 10388 13955 10388
Size: 3567 11618
ApoI r'AATT_y
Cuts at: 857 1003 1682 1873 2102 2221 2431 2854 2912
Size: 146 679 191 229 119 210 423 58
Cuts at: 2912 3427 4201 4682 4723 4848 5446 5491 5710
Size: 515 774 481 41 125 598 45 219
Cuts at: 5710 6069 6526 6538 6812 6998 7082 9837 9854
Size: 359 457 12 274 186 84 2755 17
Cuts at: 9854 10303 10444 10686 10828 10888 11089 11115 11600
Size: 449 141 242 142 60 201 26 485
Cuts at: 11600 11683 11951 12090 12189 14538 14794 857
Size: 83 268 139 99 2349 256 1248
Fragments arranged by size:
2755 2349 1248 774 679 598 515 485
481 457 449 423 359 274 268 256
242 229 219 210 201 191 186 146
142 141 139 125 119 99 84 83
60 58 45 41 26 17 12
AseI AT'TA_AT
Cuts at: 20 1774 4319 5989 7804 9998 20
Size: 1754 2545 1670 1815 2194 5207
Fragments arranged by size:
5207 2545 2194 1815 1754 1670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 15185
AvaI C'yCGr_G
Cuts at: 797 6257 12205 797
Size: 5460 5948 3777
Fragments arranged by size:
5948 5460 3777
AvrII C'CTAG_G
Cuts at: 4003 10899 4003
Size: 6896 8289
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7578 7611 9191 9224 7578
Size: 33 1580 33 13539
Fragments arranged by size:
13539 1580 33 33
BamHI G'GATC_C
Cuts at: 7091 12210 7091
Size: 5119 10066
BanI G'GyrC_C
Cuts at: 216 1563 2258 4470 8119 8962 11423 11767 12201
Size: 1347 695 2212 3649 843 2461 344 434
Cuts at: 12201 12615 12863 12898 216
Size: 414 248 35 2503
Fragments arranged by size:
3649 2503 2461 2212 1347 843 695 434
414 344 248 35
BanII G_rGCy'C
Cuts at: 3083 3263 4059 6276 8320 9782 10514 12199 13229
Size: 180 796 2217 2044 1462 732 1685 1030
Cuts at: 13229 3083
Size: 5039
Fragments arranged by size:
5039 2217 2044 1685 1462 1030 796 732
180
BbeI G_GCGC'C
Cuts at: 12619 12867 12619
Size: 248 14937
BbsI GAAGACnn'nnnn_
Cuts at: 693 826 4114 7284 9557 11503 11715 11846 693
Size: 133 3288 3170 2273 1946 212 131 4032
Fragments arranged by size:
4032 3288 3170 2273 1946 212 133 131
BciVI GTATCCnnnnn_n'
Cuts at: 3675 6939 9307 13083 13844 15185 3675
Size: 3264 2368 3776 761 1341 3675
Fragments arranged by size:
3776 3675 3264 2368 1341 761
BclI T'GATC_A
Cuts at: 3956 7494 11169 12705 3956
Size: 3538 3675 1536 6436
Fragments arranged by size:
6436 3675 3538 1536
BfrBI ATG'CAT
Cuts at: 1024 2566 7549 8243 1024
Size: 1542 4983 694 7966
Fragments arranged by size:
7966 4983 1542 694
BglI GCCn_nnn'nGGC
Cuts at: 267 7177 267
Size: 6910 8275
BglII A'GATC_T
Cuts at: 2238 4696 10935 11562 12700 14737 2238
Size: 2458 6239 627 1138 2037 2686
Fragments arranged by size:
6239 2686 2458 2037 1138 627
BlpI GC'TnA_GC
Cuts at: 1622 2207 12297 1622
Size: 585 10090 4510
Fragments arranged by size:
10090 4510 585
Bme1580I G_kGCm'C
Cuts at: 8320 10392 10514 11772 12810 12903 13959 8320
Size: 2072 122 1258 1038 93 1056 9546
Fragments arranged by size:
9546 2072 1258 1056 1038 122 93
BmgBI CAC'GTC
Cuts at: 10327 10327
Size: 15185
BmrI ACTGGGnnnn_n'
Cuts at: 2676 2891 3636 4112 5182 6056 8831 11064 12397
Size: 215 745 476 1070 874 2775 2233 1333
Cuts at: 12397 12478 12813 14957 2676
Size: 81 335 2144 2904
Fragments arranged by size:
2904 2775 2233 2144 1333 1070 874 745
476 335 215 81
BmtI G_CTAG'C
Cuts at: 2795 7967 2795
Size: 5172 10013
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3871 13552 3871
Size: 9681 5504
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2538 2557 3080 3097 3605 4997 5141 5403
Size: 508 19 523 17 508 1392 144 262
Cuts at: 5403 6374 6602 7618 8375 8812 10436 11702 2030
Size: 971 228 1016 757 437 1624 1266 5513
Fragments arranged by size:
5513 1624 1392 1266 1016 971 757 523
508 508 437 262 228 144 19 17
Bpu10I CC'TnA_GC
Cuts at: 3916 3916
Size: 15185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 612 3367 3745 5662 7892 9865 10290 10627 11121
Size: 2755 378 1917 2230 1973 425 337 494
Cuts at: 11121 12361 13240 13732 14030 612
Size: 1240 879 492 298 1767
Fragments arranged by size:
2755 2230 1973 1917 1767 1240 879 494
492 425 378 337 298
BsaI GGTCTCn'nnnn_
Cuts at: 5379 14889 5379
Size: 9510 5675
BsaAI yAC'GTr
Cuts at: 669 3065 4559 12538 669
Size: 2396 1494 7979 3316
Fragments arranged by size:
7979 3316 2396 1494
BsaBI GATnn'nnATC
Cuts at: 6 4712 5699 12699 14736 6
Size: 4706 987 7000 2037 455
Fragments arranged by size:
7000 4706 2037 987 455
BsaHI Gr'CG_yC
Cuts at: 1400 12616 12864 13566 15062 1400
Size: 11216 248 702 1496 1523
Fragments arranged by size:
11216 1523 1496 702 248
BsaWI w'CCGG_w
Cuts at: 5546 8842 10794 11764 12374 12895 13573 13847 13994
Size: 3296 1952 970 610 521 678 274 147
Cuts at: 13994 14405 5546
Size: 411 6326
Fragments arranged by size:
6326 3296 1952 970 678 610 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2662 2692 3464 3494 3520 3550 3809 3839 8963
Size: 30 772 30 26 30 259 30 5124
Cuts at: 8963 8993 9279 9309 10943 10973 11027 11057 2662
Size: 30 286 30 1634 30 54 30 6790
Fragments arranged by size:
6790 5124 1634 772 286 259 54 30
30 30 30 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1898 2718 4091 5906 6407 10636 11433 11705 12074
Size: 820 1373 1815 501 4229 797 272 369
Cuts at: 12074 1898
Size: 5009
Fragments arranged by size:
5009 4229 1815 1373 820 797 501 369
272
BseYI C'CCAG_C
Cuts at: 4411 4642 7360 8319 11214 11771 12610 13368 13945
Size: 231 2718 959 2895 557 839 758 577
Cuts at: 13945 14441 4411
Size: 496 5155
Fragments arranged by size:
5155 2895 2718 959 839 758 577 557
496 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 1187 8153 1132
Size: 55 6966 8164
Fragments arranged by size:
8164 6966 55
BsiEI CG_ry'CG
Cuts at: 104 566 8477 12727 12773 13981 104
Size: 462 7911 4250 46 1208 1308
Fragments arranged by size:
7911 4250 1308 1208 462 46
BsiHKAI G_wGCw'C
Cuts at: 1577 3263 3822 4083 4983 7073 10392 10409 12199
Size: 1686 559 261 900 2090 3319 17 1790
Cuts at: 12199 12977 13167 13959 1577
Size: 778 190 792 2803
Fragments arranged by size:
3319 2803 2090 1790 1686 900 792 778
559 261 190 17
BsmI GAATG_Cn'
Cuts at: 927 3227 4864 9060 9440 10330 11029 11078 11244
Size: 2300 1637 4196 380 890 699 49 166
Cuts at: 11244 927
Size: 4868
Fragments arranged by size:
4868 4196 2300 1637 890 699 380 166
49
Bsp1286I G_dGCh'C
Cuts at: 1577 3083 3263 3822 4059 4083 4983 6276 7073
Size: 1506 180 559 237 24 900 1293 797
Cuts at: 7073 8320 9782 10392 10409 10514 11772 12199 12810
Size: 1247 1462 610 17 105 1258 427 611
Cuts at: 12810 12903 12977 13167 13229 13959 1577
Size: 93 74 190 62 730 2803
Fragments arranged by size:
2803 1506 1462 1293 1258 1247 900 797
730 611 610 559 427 237 190 180
105 93 74 62 24 17
BspEI T'CCGG_A
Cuts at: 8842 10794 14405 8842
Size: 1952 3611 9622
Fragments arranged by size:
9622 3611 1952
BspHI T'CATG_A
Cuts at: 1055 1316 1403 2550 6735 6843 8364 9416 14361
Size: 261 87 1147 4185 108 1521 1052 4945
Cuts at: 14361 1055
Size: 1879
Fragments arranged by size:
4945 4185 1879 1521 1147 1052 261 108
87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4315 5427 8107 11311 12235 12449 12751 13132 4315
Size: 1112 2680 3204 924 214 302 381 6368
Fragments arranged by size:
6368 3204 2680 1112 924 381 302 214
BsrBI CCG'CTC
Cuts at: 50 9990 12054 13477 13531 15015 15180 50
Size: 9940 2064 1423 54 1484 165 55
Fragments arranged by size:
9940 2064 1484 1423 165 55 54
BsrDI GCAATG_nn'
Cuts at: 932 2579 4469 6602 7497 8510 10514 11701 13097
Size: 1647 1890 2133 895 1013 2004 1187 1396
Cuts at: 13097 932
Size: 3020
Fragments arranged by size:
3020 2133 2004 1890 1647 1396 1187 1013
895
BsrFI r'CCGG_y
Cuts at: 9920 11060 11764 13183 13364 13573 9920
Size: 1140 704 1419 181 209 11532
Fragments arranged by size:
11532 1419 1140 704 209 181
BsrGI T'GTAC_A
Cuts at: 5689 8807 10066 5689
Size: 3118 1259 10808
Fragments arranged by size:
10808 3118 1259
BssSI C'ACGA_G
Cuts at: 13456 13814 13456
Size: 358 14827
BstAPI GCAn_nnn'nTGC
Cuts at: 12562 12562
Size: 15185
BstBI TT'CG_AA
Cuts at: 6984 8988 13546 6984
Size: 2004 4558 8623
Fragments arranged by size:
8623 4558 2004
BstEII G'GTnAC_C
Cuts at: 2692 2692
Size: 15185
BstXI CCAn_nnnn'nTGG
Cuts at: 872 942 2086 4128 9639 9708 10465 11612 11726
Size: 70 1144 2042 5511 69 757 1147 114
Cuts at: 11726 872
Size: 4331
Fragments arranged by size:
5511 4331 2042 1147 1144 757 114 70
69
BstYI r'GATC_y
Cuts at: 1 792 2238 2962 3369 4696 7091 9081 9237
Size: 791 1446 724 407 1327 2395 1990 156
Cuts at: 9237 10422 10935 11562 12210 12677 12700 13035 13281
Size: 1185 513 627 648 467 23 335 246
Cuts at: 13281 14293 14379 14391 14737 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2395 1990 1446 1327 1185 1012 791 724
648 627 513 467 449 407 346 335
246 156 86 23 12
BstZ17I GTA'TAC
Cuts at: 6479 6479
Size: 15185
Bsu36I CC'TnA_GG
Cuts at: 3119 4085 9428 10823 11864 3119
Size: 966 5343 1395 1041 6440
Fragments arranged by size:
6440 5343 1395 1041 966
BtgI C'CryG_G
Cuts at: 615 5060 5890 8588 10427 615
Size: 4445 830 2698 1839 5373
Fragments arranged by size:
5373 4445 2698 1839 830
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1883 2833 3899 8117 13135 13316 13479 1883
Size: 950 1066 4218 5018 181 163 3589
Fragments arranged by size:
5018 4218 3589 1066 950 181 163
BtsI GCAGTG_nn'
Cuts at: 120 1173 2855 4523 5204 12428 120
Size: 1053 1682 1668 681 7224 2877
Fragments arranged by size:
7224 2877 1682 1668 1053 681
ClaI AT'CG_AT
Cuts at: 5703 5703
Size: 15185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3046 3081 5378 5413 12075 12110 3046
Size: 35 2297 35 6662 35 6121
Fragments arranged by size:
6662 6121 2297 35 35 35
DraI TTT'AAA
Cuts at: 788 975 5042 5775 6080 7247 12184 788
Size: 187 4067 733 305 1167 4937 3789
Fragments arranged by size:
4937 4067 3789 1167 733 305 187
DrdI GACnn_nn'nnGTC
Cuts at: 532 2722 5473 12891 13749 532
Size: 2190 2751 7418 858 1968
Fragments arranged by size:
7418 2751 2190 1968 858
EaeI y'GGCC_r
Cuts at: 131 8147 8474 8642 12016 12078 12770 12944 13335
Size: 8016 327 168 3374 62 692 174 391
Cuts at: 13335 13362 14707 131
Size: 27 1345 609
Fragments arranged by size:
8016 3374 1345 692 609 391 327 174
168 62 27
EagI C'GGCC_G
Cuts at: 8474 12770 8474
Size: 4296 10889
EarI CTCTTCn'nnn_
Cuts at: 2327 3454 5151 7316 8194 8919 9260 9671 12116
Size: 1127 1697 2165 878 725 341 411 2445
Cuts at: 12116 13208 13418 2327
Size: 1092 210 4094
Fragments arranged by size:
4094 2445 2165 1697 1127 1092 878 725
411 341 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 13393 13703 13849 14995 13393
Size: 310 146 1146 13583
Fragments arranged by size:
13583 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3261 12197 3261
Size: 8936 6249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2030 2182 2227 2351 2538 2557 3010 3080 3097
Size: 152 45 124 187 19 453 70 17
Cuts at: 3097 3605 4459 4672 4997 5132 5141 5403 6374
Size: 508 854 213 325 135 9 262 971
Cuts at: 6374 6602 7117 7618 7643 8375 8588 8812 9856
Size: 228 515 501 25 732 213 224 1044
Cuts at: 9856 10235 10436 10552 11515 11702 13010 13442 14189
Size: 379 201 116 963 187 1308 432 747
Cuts at: 14189 14457 2030
Size: 268 2758
Fragments arranged by size:
2758 1308 1044 971 963 854 747 732
515 508 501 453 432 379 325 268
262 228 224 213 213 201 187 187
152 135 124 116 70 45 25 19
17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2266 3122 5834 11994 2266
Size: 856 2712 6160 5457
Fragments arranged by size:
6160 5457 2712 856
EcoO109I rG'GnC_Cy
Cuts at: 1227 5115 6385 6741 8316 10510 12324 1227
Size: 3888 1270 356 1575 2194 1814 4088
Fragments arranged by size:
4088 3888 2194 1814 1575 1270 356
EcoRI G'AATT_C
Cuts at: 5446 10828 12189 5446
Size: 5382 1361 8442
Fragments arranged by size:
8442 5382 1361
EcoRV GAT'ATC
Cuts at: 996 1380 8829 8931 11253 996
Size: 384 7449 102 2322 4928
Fragments arranged by size:
7449 4928 2322 384 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1617 4464 6624 8593 8677 9463 9519 9906 9966
Size: 2847 2160 1969 84 786 56 387 60
Cuts at: 9966 13204 1617
Size: 3238 3598
Fragments arranged by size:
3598 3238 2847 2160 1969 786 387 84
60 56
FspI TGC'GCA
Cuts at: 11074 12966 11074
Size: 1892 13293
HaeII r_GCGC'y
Cuts at: 12619 12867 13889 14868 12619
Size: 248 1022 979 12936
Fragments arranged by size:
12936 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 511 543 2628 2660 2662 2694 3091 3123 3340
Size: 32 2085 32 2 32 397 32 217
Cuts at: 3340 3354 3372 3386 3705 3737 3841 3873 4567
Size: 14 18 14 319 32 104 32 694
Cuts at: 4567 4599 4896 4928 5077 5109 5604 5636 5785
Size: 32 297 32 149 32 495 32 149
Cuts at: 5785 5817 5994 6026 6708 6740 7359 7391 7990
Size: 32 177 32 682 32 619 32 599
Cuts at: 7990 8022 8935 8967 9066 9069 9098 9101 10757
Size: 32 913 32 99 3 29 3 1656
Cuts at: 10757 10771 10776 10789 10803 10808 13027 13057 13059
Size: 14 5 13 14 5 2219 30 2
Cuts at: 13059 13089 511
Size: 30 2607
Fragments arranged by size:
2607 2219 2085 1656 913 694 682 619
599 495 397 319 297 217 177 149
149 104 99 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 29 18 14
14 14 14 13 5 5 3 3
2 2
HincII GTy'rAC
Cuts at: 2037 7587 8706 10640 12169 12224 2037
Size: 5550 1119 1934 1529 55 4998
Fragments arranged by size:
5550 4998 1934 1529 1119 55
HindIII A'AGCT_T
Cuts at: 7713 8221 7713
Size: 508 14677
HpaI GTT'AAC
Cuts at: 7587 7587
Size: 15185
KasI G'GCGC_C
Cuts at: 12615 12863 12615
Size: 248 14937
KpnI G_GTAC'C
Cuts at: 2262 4474 8966 11427 12205 2262
Size: 2212 4492 2461 778 5242
Fragments arranged by size:
5242 4492 2461 2212 778
MfeI C'AATT_G
Cuts at: 1147 4833 9317 1147
Size: 3686 4484 7015
Fragments arranged by size:
7015 4484 3686
MluI A'CGCG_T
Cuts at: 14618 14618
Size: 15185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 402 2273 2906 4160 4911 7113 7913 13856 14040
Size: 1871 633 1254 751 2202 800 5943 184
Cuts at: 14040 402
Size: 1547
Fragments arranged by size:
5943 2202 1871 1547 1254 800 751 633
184
MscI TGG'CCA
Cuts at: 8644 12080 12946 8644
Size: 3436 866 10883
Fragments arranged by size:
10883 3436 866
MslI CAynn'nnrTG
Cuts at: 296 920 1054 1661 2632 3379 3775 6712 8369
Size: 624 134 607 971 747 396 2937 1657
Cuts at: 8369 8503 9706 10519 12162 13174 13301 296
Size: 134 1203 813 1643 1012 127 2180
Fragments arranged by size:
2937 2180 1657 1643 1203 1012 971 813
747 624 607 396 134 134 127
MspA1I CmG'CkG
Cuts at: 177 240 532 1221 2256 3142 4096 4581 7437
Size: 63 292 689 1035 886 954 485 2856
Cuts at: 7437 8473 8566 8805 10811 11280 12296 12610 12970
Size: 1036 93 239 2006 469 1016 314 360
Cuts at: 12970 13983 14228 177
Size: 1013 245 1134
Fragments arranged by size:
2856 2006 1134 1036 1035 1016 1013 954
886 689 485 469 360 314 292 245
239 93 63
NaeI GCC'GGC
Cuts at: 13366 13366
Size: 15185
NarI GG'CG_CC
Cuts at: 12616 12864 12616
Size: 248 14937
NcoI C'CATG_G
Cuts at: 615 5060 5890 8588 10427 615
Size: 4445 830 2698 1839 5373
Fragments arranged by size:
5373 4445 2698 1839 830
NdeI CA'TA_TG
Cuts at: 2562 8514 2562
Size: 5952 9233
NgoMIV G'CCGG_C
Cuts at: 13364 13364
Size: 15185
NheI G'CTAG_C
Cuts at: 2791 7963 2791
Size: 5172 10013
NsiI A_TGCA'T
Cuts at: 1026 2568 7551 8245 1026
Size: 1542 4983 694 7966
Fragments arranged by size:
7966 4983 1542 694
NspI r_CATG'y
Cuts at: 3196 3746 4379 5376 6429 6831 7488 7519 7707
Size: 550 633 997 1053 402 657 31 188
Cuts at: 7707 9296 10968 12238 12738 13645 3196
Size: 1589 1672 1270 500 907 4736
Fragments arranged by size:
4736 1672 1589 1270 1053 997 907 657
633 550 500 402 188 31
PasI CC'CwG_GG
Cuts at: 4402 8967 4402
Size: 4565 10620
PciI A'CATG_T
Cuts at: 3192 3742 4375 5372 6827 7484 7703 9292 10964
Size: 550 633 997 1455 657 219 1589 1672
Cuts at: 10964 13641 3192
Size: 2677 4736
Fragments arranged by size:
4736 2677 1672 1589 1455 997 657 633
550 219
PflMI CCAn_nnn'nTGG
Cuts at: 2666 6397 7604 10592 11472 2666
Size: 3731 1207 2988 880 6379
Fragments arranged by size:
6379 3731 2988 1207 880
PfoI T'CCnGG_A
Cuts at: 7087 10413 11908 7087
Size: 3326 1495 10364
Fragments arranged by size:
10364 3326 1495
PmeI GTTT'AAAC
Cuts at: 12184 12184
Size: 15185
PmlI CAC'GTG
Cuts at: 3065 3065
Size: 15185
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 14324 14356 14324
Size: 32 15153
PpuMI rG'GwC_Cy
Cuts at: 1227 5115 6385 6741 1227
Size: 3888 1270 356 9671
Fragments arranged by size:
9671 3888 1270 356
PshAI GACnn'nnGTC
Cuts at: 4536 4536
Size: 15185
PsiI TTA'TAA
Cuts at: 4390 6445 6524 10309 4390
Size: 2055 79 3785 9266
Fragments arranged by size:
9266 3785 2055 79
PspOMI G'GGCC_C
Cuts at: 8316 10510 8316
Size: 2194 12991
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5751 5783 9242 9274 5751
Size: 32 3459 32 11662
Fragments arranged by size:
11662 3459 32 32
PstI C_TGCA'G
Cuts at: 1226 2307 8357 8565 10709 12232 12917 1226
Size: 1081 6050 208 2144 1523 685 3494
Fragments arranged by size:
6050 3494 2144 1523 1081 685 208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 15185
PvuII CAG'CTG
Cuts at: 177 240 532 1221 3142 4096 4581 8566 8805
Size: 63 292 689 1921 954 485 3985 239
Cuts at: 8805 10811 11280 12610 12970 177
Size: 2006 469 1330 360 2392
Fragments arranged by size:
3985 2392 2006 1921 1330 954 689 485
469 360 292 239 63
RsrII CG'GwC_CG
Cuts at: 13380 13380
Size: 15185
SacI G_AGCT'C
Cuts at: 3263 12199 3263
Size: 8936 6249
SalI G'TCGA_C
Cuts at: 12222 12222
Size: 15185
SapI GCTCTTCn'nnn_
Cuts at: 3454 13208 13418 3454
Size: 9754 210 5221
Fragments arranged by size:
9754 5221 210
SbfI CC_TGCA'GG
Cuts at: 12232 12232
Size: 15185
ScaI AGT'ACT
Cuts at: 486 4274 10703 14702 486
Size: 3788 6429 3999 969
Fragments arranged by size:
6429 3999 3788 969
SexAI A'CCwGG_T
Cuts at: 8699 9802 8699
Size: 1103 14082
SfcI C'TryA_G
Cuts at: 33 575 1222 2303 2445 4139 6137 6954 7730
Size: 542 647 1081 142 1694 1998 817 776
Cuts at: 7730 8009 8353 8468 8561 8880 10705 11558 12228
Size: 279 344 115 93 319 1825 853 670
Cuts at: 12228 12913 13906 14097 33
Size: 685 993 191 1121
Fragments arranged by size:
1998 1825 1694 1121 1081 993 853 817
776 685 670 647 542 344 319 279
191 142 115 93
SfoI GGC'GCC
Cuts at: 12617 12865 12617
Size: 248 14937
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 15185
SmaI CCC'GGG
Cuts at: 12207 12207
Size: 15185
SmlI C'TyrA_G
Cuts at: 591 797 3346 3724 5641 7257 7907 8620 9880
Size: 206 2549 378 1917 1616 650 713 1260
Cuts at: 9880 10269 10606 11136 12340 13255 13747 14009 591
Size: 389 337 530 1204 915 492 262 1767
Fragments arranged by size:
2549 1917 1767 1616 1260 1204 915 713
650 530 492 389 378 337 262 206
SnaBI TAC'GTA
Cuts at: 4559 4559
Size: 15185
SpeI A'CTAG_T
Cuts at: 67 4954 6354 67
Size: 4887 1400 8898
Fragments arranged by size:
8898 4887 1400
SphI G_CATG'C
Cuts at: 12238 12738 12238
Size: 500 14685
SspI AAT'ATT
Cuts at: 1119 2803 3286 3879 6026 6159 6441 8132 8385
Size: 1684 483 593 2147 133 282 1691 253
Cuts at: 8385 9000 10911 11523 1119
Size: 615 1911 612 4781
Fragments arranged by size:
4781 2147 1911 1691 1684 615 612 593
483 282 253 133
StuI AGG'CCT
Cuts at: 8753 9637 8753
Size: 884 14301
StyI C'CwwG_G
Cuts at: 615 871 1128 4003 4071 4121 5060 5890 8588
Size: 256 257 2875 68 50 939 830 2698
Cuts at: 8588 8754 9632 9638 10361 10427 10506 10899 12128
Size: 166 878 6 723 66 79 393 1229
Cuts at: 12128 12319 12673 615
Size: 191 354 3127
Fragments arranged by size:
3127 2875 2698 1229 939 878 830 723
393 354 257 256 191 166 79 68
66 50 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2780 5394 6022 11062 12768 12901 13360 13569 2780
Size: 2614 628 5040 1706 133 459 209 4396
Fragments arranged by size:
5040 4396 2614 1706 628 459 209 133
TatI w'GTAC_w
Cuts at: 253 484 948 1718 3690 4272 5689 8807 9289
Size: 231 464 770 1972 582 1417 3118 482
Cuts at: 9289 10066 10701 11457 14700 253
Size: 777 635 756 3243 738
Fragments arranged by size:
3243 3118 1972 1417 777 770 756 738
635 582 482 464 231
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3809 3841 5804 5836 8963 8995 9320 9352 9446
Size: 32 1963 32 3127 32 325 32 94
Cuts at: 9446 9478 11027 11059 11741 11773 14702 14734 3809
Size: 32 1549 32 682 32 2929 32 4260
Fragments arranged by size:
4260 3127 2929 1963 1549 682 325 94
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 9166 12982 9166
Size: 3816 11369
XbaI T'CTAG_A
Cuts at: 6490 6543 12216 6490
Size: 53 5673 9459
Fragments arranged by size:
9459 5673 53
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3522 3580 5165 7923 12680 3522
Size: 58 1585 2758 4757 6027
Fragments arranged by size:
6027 4757 2758 1585 58
XhoI C'TCGA_G
Cuts at: 797 797
Size: 15185
XmaI C'CCGG_G
Cuts at: 12205 12205
Size: 15185
XmnI GAAnn'nnTTC
Cuts at: 700 4761 6839 8463 9254 11665 700
Size: 4061 2078 1624 791 2411 4220
Fragments arranged by size:
4220 4061 2411 2078 1624 791
ZraI GAC'GTC
Cuts at: 1401 1401
Size: 15185
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BbvCI BcgI BsiWI BsmBI BssHII DraIII
FseI FspAI NotI NruI PacI PspXI SacII SanDI
SfiI SgrAI SrfI SwaI